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101 results about "Dormancy" patented technology

Dormancy is a period in an organism's life cycle when growth, development, and (in animals) physical activity are temporarily stopped. This minimizes metabolic activity and therefore helps an organism to conserve energy. Dormancy tends to be closely associated with environmental conditions. Organisms can synchronize entry to a dormant phase with their environment through predictive or consequential means. Predictive dormancy occurs when an organism enters a dormant phase before the onset of adverse conditions. For example, photoperiod and decreasing temperature are used by many plants to predict the onset of winter. Consequential dormancy occurs when organisms enter a dormant phase after adverse conditions have arisen. This is commonly found in areas with an unpredictable climate. While very sudden changes in conditions may lead to a high mortality rate among animals relying on consequential dormancy, its use can be advantageous, as organisms remain active longer and are therefore able to make greater use of available resources.

Medlar planting distribution high-precision remote sensing monitoring method based on multi-temporal fusion and spectral feature optimization

The invention belongs to the technical field of remote sensing, and discloses a wolfberry planting distribution high-precision remote sensing monitoring method based on multi-temporal fusion and spectral feature optimization. According to the method, a multi-temporal and multi-spectral satellite image is used as a data source, and preprocessing and multi-temporal image fusion are firstly carried out; the method comprises the following core steps: establishing a time sequence characteristic curve according to a unique phenological period (such as bare soil characteristics in a dormancy period and high vegetation coverage in a rapid growth period) of wolfberry; in a spectral domain, screening out a characteristic spectrum dimension combination with the highest discrimination degree between the wolfberry and other crops through a characteristic wave band optimization algorithm (such as vegetation index difference degree and red edge characteristics); and in combination with an object-oriented classification or deep learning classification model, constructing a space-time coupling classifier, and performing high-precision extraction and distribution mapping on the Chinese wolfberry planting region. The method can effectively solve the problem of confusion classification of Chinese wolfberry and similar ground features (such as other shrubs and orchards), and realizes rapid and accurate monitoring of Chinese wolfberry planting area and spatial distribution.
Owner:INST OF PLANT PROTECTION NINGXIA ACAD OF AGRI & FORESTRY SCI KEY LAB OF NINGXIA PLANT DISEASE & INSECT PESTS CONTROL

Application of knocking down or knocking out osaba8ox2 protein or its coding gene in improving seed panicle germination resistance of rice

PendingCN122278924ABiotechnologyNucleotide
This invention discloses the application of knocking down or knocking out the OsABA8ox2 protein or its encoding gene in improving the panicle germination resistance of rice seeds, belonging to the fields of biotechnology and plant genetic engineering technology. OsABA8ox2 The amino acid sequence of the protein is shown in SEQ ID NO.2, and the nucleotide sequence of the gene is shown in SEQ ID NO.1. This invention utilizes CRISPR / Cas9 technology to directionally edit the target site sequence in this gene, as shown in SEQ ID NO.3, successfully obtaining rice nucleotide sequences as shown in SEQ ID NO.8, SEQ ID NO.9, and SEQ ID NO.10. OsABA8ox2 The mutant, to a certain extent, enhances the dormancy of rice seeds, effectively reducing the germination rate of rice panicles. The gene resources and gene editing technology provided by this invention offer an important technical approach for rapidly improving or creating rice varieties with significantly enhanced panicle germination resistance in agricultural production, demonstrating significant application value in production practice.
Owner:YANGZHOU UNIV +1

Method for improving production efficiency of gastrodia elata f.glauca

The invention belongs to the technical field of edible fungus cultivation, and particularly relates to a method for improving the production efficiency of gastrodia elata f.glauca. The whole cultivation period of the gastrodia elata f.glauca can be effectively shortened, and the cultivation period can be shortened; according to the method, the bacteria retreating phenomenon of the armillaria mellea can be effectively reduced, compared with a traditional cultivation fungus bed, the armillaria mellea is only punched and is not completely opened, and even if part of the armillaria mellea dies in the cultivation process, new armillaria mellea can germinate in the bag to promote growth of gastrodia elata and prevent bacteria retreating; the high-nutrition armillaria mellea stick is prepared, and the formula nutrition of the armillaria mellea stick is suitable for supplementing organic nitrogen lacking in wood and can promote growth of armillaria mellea; when gastrodia elata f.glauca is planted, the biggest risk point is that armillaria mellea cannot be inoculated with wood and gastrodia elata seeds, the gastrodia elata seed dormancy breaking nutrient solution and the wood pretreatment nutrient solution are adopted, the risk can be effectively avoided, and the overall planting risk is reduced.
Owner:GUIZHOU JINCHAN DASHAN BIOTECHNOLOGY CO LTD

Rapid dormancy breaking method for sand penis seeds and dormancy breaking sand penis seeds thereof

The invention provides a rapid dormancy breaking method for sand whip seeds and dormancy breaking sand whip seeds, and relates to the technical field of plant seed treatment.The method comprises the following steps that 1, physical initiation treatment is conducted, specifically, the disinfected sand whip seeds are placed in warm water at the temperature of 45-55 DEG C to be soaked for 10-14 h; (2) chemical initiation treatment: soaking the seeds treated in the step (1) in a gibberellin solution with the mass concentration of 0.03%-0.07% for 20-28 hours; (3) biological seed dressing treatment: carrying out seed dressing on the seeds treated in the step (2) and a trichoderma harzianum fungicide, wherein the mass of the fungicide is 0.05-0.15% of the mass of the seeds; according to the method, the dormancy mechanism of the sand penis seeds can be effectively broken, germination of the seeds is promoted, the germination rate of the sand penis seeds treated by the optimized method can reach 87.00% on the 21st day, the germination synchronism of the seeds can be higher, and the problem of irregular natural germination is solved.
Owner:INNER MONGOLIA MENGCAO GRASS IND TECHNOLOGY CO LTD +1

Culture method and application of corn seed embryos

The invention provides a non-dormancy direct germination technology for corn seeds, which can enable immature corn seed embryos to directly germinate and grow into plants without induction treatment of an MS culture medium, disinfection pretreatment of corn ears and the like. The method can accelerate seed germination and effectively shorten the corn growth cycle. According to the non-dormancy direct germination technology for the corn seeds, the germination rate can reach 97%, the seedling rate can reach 97%, and technical support is provided for genetic breeding or physiological research.
Owner:BEIJING JINGXIU YUANQI BIOTECHNOLOGY CO LTD

A SSR molecular marker primer related to leek dormancy and a detection method and application thereof

The application discloses a SSR molecular marker primer related to Chinese chives dormancy and a detection method and application thereof. In view of the problems that traditional Chinese chives dormancy trait identification relies on field morphological observation for 4 months, is inefficient and lacks special molecular markers, 7 specific SSR primers (SSR005 / 170 / 249 / 325 / 423 / 478 / 610) are developed based on cross-species genome resequencing (taking Chinese onion as a reference genome, and the alignment rate is 18.76%-18.89%) for the first time. Through the establishment of a matching detection system: CTAB method for rapid DNA extraction, optimization of the PCR system (20 muL contains 30 ng of template DNA, 0.4 U of Taq enzyme, 0.2 muM of primer, 0.2 mM of Mg 2+ 2.0 mM of dNTPs, 57 DEG C annealing), 8% polyacrylamide gel electrophoresis combined with silver staining development, the dormancy trait can be accurately identified within 7 days. For example, the SSR423 primer has no band at 177 bp, and the non-dormant / dormant germplasm cannot be distinguished. The detection results of 52 germplasms (such as dormancy germplasm 21-1 and non-dormancy germplasm 22-7) are consistent with the field phenotypes at a rate of 100%.
Owner:河北省农林科学院经济作物研究所

A method for shrimp non-aqueous live-keeping transportation

The present application belongs to the field of live transportation of aquatic products, and relates to a method for live transportation of prawns without water. The core of the method is to use a staged treatment strategy and the synergistic effect of dormancy induction liquid and moisture supply liquid. First, the prawns are adapted to the environment by fasting and gradient cooling, then they are soaked in pre-cooled dormancy induction liquid to enter a pseudo-death dormancy state and reduce metabolism. Then, the moisture supply liquid soaked sponge is used as a transportation medium, which can form a protective film on the surface of the prawn body to effectively prevent water loss and provide energy. During transportation, the prawns treated with the moisture supply liquid are placed in a sealed box filled with pure oxygen together with the moist sponge, and the prawns are transported without water at low temperature. After the transportation is completed, the prawns are placed in warm water to quickly recover. The prawn live transportation method without water prolongs the survival time of prawns in a water-free environment, improves the transportation efficiency, is simple to operate, and is suitable for large-scale long-distance transportation of prawns.
Owner:SHANDONG HENGXING FISHERY DEV CO LTD +1

Method for improving germination rate of hemerocallis citrina baroni seeds

The invention relates to the technical field of agricultural biology, in particular to a method for increasing the germination rate of hemerocallis citrina L. seeds, the method for remarkably increasing the germination rate of the hemerocallis citrina L. seeds through combined treatment of gibberellin GA3 and hydrogen peroxide H2O2 is easy and convenient to operate and low in cost, the physiological dormancy of the seeds is broken through gibberellin, embryo cell division is promoted, and the germination rate of the seeds is increased. Meanwhile, hydrogen peroxide is used for moderately oxidizing seed coats, permeability is improved, active oxygen signal molecules are provided for activating germination related enzyme systems, the hydrogen peroxide and the active oxygen signal molecules generate a synergistic interaction effect through a dormancy breaking-permeation promoting-activation triple mechanism, the germination rate is increased in a breakthrough mode, and the germination period is remarkably shortened.
Owner:SHANXI AGRI UNIV

Method for establishing a tissue culture sterile system of peach rootstock dormancy material

The present application relates to the technical field of plant tissue culture, and specifically discloses a method for establishing a tissue culture sterile system of peach rootstock dormancy material, which comprises the following steps: S1, selecting one-year-old branches with leaf buds in the dormancy period, removing flower buds and cleaning; S2, performing low-temperature treatment on the branches, then performing water culture and bud breaking to obtain sprouting branches; S3, performing surface sterilization treatment on the sprouting branches, and then washing with sterile water; S4, peeling the sprouting buds from the branches under sterile conditions, removing the external wrapping tissues of the bud bodies, and exposing the internal tender tissues; S5, performing secondary sterilization treatment on the exposed tender tissues, and then washing with sterile water; and S6, inoculating the bud bodies subjected to the secondary sterilization treatment into a culture medium for culture, and establishing a sterile system; according to the above method, in the process of establishing the sterile system of Zhongtao Kangzhen No. 1 material, the sterilization survival rate of 100 bud heads is as high as 80% or more, and the work efficiency and the utilization efficiency of the material are greatly improved.
Owner:LINYI AGRIFA SEEDS CO LTD

Nectarine tree named ‘TRH4D144WN’

ActiveUSPP37640P2PrunusDormancy
Disclosed is a new variety of Prunus persica named ‘TRH4D144WN’, which requires approximately 500 chilling units of dormancy, produces an acid-sweet, white-fleshed firm clingstone nectarine with a 70-90% medium to dark pink blush that matures in the early mid-season.
Owner:TEXAS A&M UNIVERSITY

Migrant bird type fruit tree aeroponic cultivation method and device

The invention belongs to the technical field of agricultural planting, and provides a migrant bird type fruit tree aeroponic cultivation method and device.The method comprises the steps that a sapling to be migrated is cultivated in the north to form a tree body with the flower bud differentiation capacity; wrapping the root system of the tree body and then putting the tree body into a refrigeration house, so that the tree body performs cold storage and dormancy; transporting the tree body after cold storage and dormancy from the north to the south; and planting the dormant tree bodies in the southern region, so that the tree bodies blossom and bear fruits. The method can be suitable for anti-season, super-early-maturing or peak-shifting production scenes of northern deciduous fruit trees which can break dormancy only through cold stimulation at a low temperature of a certain hours in winter.
Owner:LISHUI UNIV

Soil remediation equipment utilizing microorganisms

The utility model discloses soil remediation equipment utilizing microorganisms, and belongs to the field of soil remediation. Comprising a rotary cultivator used for turning up soil; the microorganism cultivation tank is used for cultivating microorganisms; the feeding assembly is arranged on the rotary cultivator and used for feeding the microorganisms in the microorganism cultivation tank into soil; the flow switch is arranged on the microorganism cultivation tank, an outlet of the flow switch is connected with a feeding pipe, and the other end of the feeding pipe is connected with the feeding assembly; an inner cavity of the microorganism cultivation tank comprises a dormancy area cavity and a cultivation area cavity, the dormancy area cavity is communicated with the bottom of the cultivation area cavity through a pipeline, dormancy gas is arranged in the dormancy area, a growth lamp strip is arranged in the cultivation area, and a microbial agent inlet communicated with the cultivation area cavity is formed in the microorganism cultivation tank. The soil remediation equipment has the beneficial effect that the soil remediation equipment utilizing the microorganisms is provided.
Owner:DADI WATER ECOLOGICAL MANAGEMENT (ZHEJIANG) CO LTD

Application of fringed iris IjFLCL1 gene in regulation and control of fringed iris growth and ecological dormancy conversion

The invention discloses application of a fringed iris IjFLCL1 gene to regulation and control of fringed iris growth and ecological dormancy conversion. When the fringed iris needs to be kept in an ecological dormancy state, the IjFLCL1 gene is silenced or knocked out; when the fringed iris needs to be inhibited from entering the ecological dormancy state, the IjFLCL1 gene is subjected to overexpression; the nucleotide sequence of a protein coding region of the fringed iris IjFLCL1 gene is as shown in SEQ ID No. 1. According to the invention, a homologous gene function verification method is adopted in a plant for the first time, and it is proved that silence or knockout of the IjFLCL1 gene can maintain fringed fringed iris in an ecological dormancy state. The gene has the highest expression quantity in the spring growth period and the lowest expression quantity in the dormancy induction period. Silence or knockout of the gene can delay removal of ecological dormancy of fringed iris and help plants avoid adverse effects caused by early spring climate fluctuation, so that safety and economic benefits in the cultivation process are improved.
Owner:LISHUI GREENLAND EAST CHINA PHARMACEUTICAL BOTANICAL GARDEN CO LTD +1

Polygonatum kingianum tissue culture medium and application thereof

The invention provides a polygonatum kingianum tissue culture medium and application thereof, and belongs to the technical field of plant tissue culture. The polygonatum kingianum tissue culture medium comprises a seed induction medium, a cluster bud induction medium, a proliferation medium and a rooting medium. The seed induction culture medium can promote seed germination and expansion; the cluster bud induction culture medium can improve the activity of the explant, so that the explant forms a large number of cluster buds in a relatively short time; the proliferation culture medium can induce and generate a large number of healthy buds, and the expansion condition of the rhizomes can be obvious; the rooting culture medium can effectively improve the rooting rate and promote the root system to grow well and uniformly. The polygonatum kingianum tissue culture medium provided by the invention breaks the dormancy mechanism of polygonatum kingianum seeds, shortens the germination time of the polygonatum kingianum seeds, can obtain a large number of cluster buds in a short time, further obtains seedlings, and is beneficial to tissue culture and rapid propagation of the polygonatum kingianum seeds.
Owner:YIBIN UNIV +1

Chinese chive SSR-PCR reaction technical system and application thereof

The invention discloses a leek SSR-PCR reaction system and application thereof, and solves the technical bottlenecks of long period (more than or equal to 4 months), high error rate (more than or equal to 15%) and low flux (less than 10 parts per day) caused by the fact that traditional dormancy character identification depends on field phenotype observation. The system takes a precisely optimized 20 [mu] L reaction system as a core, comprises 30 ng of template DNA, 0.4 U of Taq enzyme, 0.2 [mu] M of primer, 2.0 mM of Mg < 2 + > and 0.2 mM of dNTPs, and realizes stable amplification by combining with a 57 DEG C annealing temperature program; seven SSR molecular markers linked with dormancy characters are synchronously developed, and the SSR423 marker judges dormancy phenotypes through 177bp strip deletion. During application, DNA is extracted through a CTAB method, after amplification of the system, germplasm typing can be completed within 8 hours through polyacrylamide gel electrophoresis detection, 52 germplasm verification shows that the consistency with field phenotypes reaches 100%, and the flux is increased to be larger than 200 parts per day. The technology provides an efficient molecular tool for breeding of leek dormancy-resistant varieties, and annual balanced supply of the market is guaranteed.
Owner:河北省农林科学院经济作物研究所

An integrated method for catching, transporting and domesticating a species of poecilia reticulata

PendingCN122271255APoeciliaBarb
This invention relates to the field of endangered aquatic biological resource protection and aquaculture technology, specifically to an integrated method for the harvesting, transportation, and domestication of the wavy-lipped barb, comprising the following steps: S1, behavioral adaptation-based nocturnal dive-fishing and decompression: during the wavy-lipped barb's nocturnal dormancy period, the target fish are gently induced into a pressure-regulating container; the fish are then safely transported to the water surface; S2, multi-stage live-preservation transportation: the fish treated in S1 are first transported via a live-water vessel for rehabilitation at sea, and then smoothly transferred via a live-water truck on land; S3, gradient-adaptive artificial domestication; S4, live germplasm resource preservation. This invention ensures that wild wavy-lipped barbs receive physiological protection at each transfer point, maximizing the maintenance of their internal environmental homeostasis, thereby increasing the overall survival rate to nearly 100% and providing high-quality, healthy parent fish for subsequent artificial breeding.
Owner:HAINAN ACADEMY OF OCEAN & FISHERIES SCI

Rice bud stage salt tolerance identification method and application thereof

The invention provides a rice bud stage salt tolerance identification method and application thereof, and the method comprises the following steps: sterilizing, drying and dormancy breaking treatment are performed on rice seeds in sequence, salt stress treatment is performed, and water soaking treatment is taken as a contrast; germination accelerating and germination, counting the germination rate and calculating the relative salt damage rate, and performing salt tolerance grading according to the relative salt damage rate; the dormancy breaking comprises two stages, the dormancy breaking temperature of the first stage is-4 to-10 DEG C, the dormancy breaking time is 12-24 hours, the dormancy breaking temperature of the second stage is-10 to-20 DEG C, and the dormancy breaking time is 24-36 hours. According to the method, efficient treatment of small-scale or large-scale rice materials can be realized under limited experimental conditions, the reliability and comparability of salt tolerance surface identification results are ensured, and a solid and reliable phenotypic basis is provided for rice salt tolerance research and molecular breeding.
Owner:SOUTH CHINA AGRICULTURAL UNIVERSITY +1

Application of LUX gene in regulating the growth of poplar terminal buds

ActiveCN119685373BDelayed growth cessationshorten the timeBiotechnologyBud
The application belongs to the field of genetic engineering, and particularly relates to application of a LUX gene in regulating growth of a poplar top bud. Through screening and testing, it is found that knocking out the LUX gene can achieve the technical effects of delaying growth stop and dormancy time of a tree top bud under short-day conditions and promoting growth of the tree.
Owner:HUAZHONG AGRI UNIV

A high-temperature germination and drought-rehydration method for indoor incubation of flower buds to improve crocus sativus stigma yield and quality

The application provides a high-temperature germination and drought after rehydration indoor incubation flower bud method capable of improving crocus sativus L. yield and quality. Compared with a traditional two-stage cultivation method, the method initiates a high-temperature cumulative dormancy breaking method during crocus sativus L. indoor cultivation flowering period, not only promotes crocus sativus L. early flowering, but also increases crocus sativus L. flowering number, advances root system development time by two months, significantly prolongs corm vegetative growth period, and improves daughter corm yield. Meanwhile, the method can effectively increase crocus sativus L. stigma length, weight, improve crocus sativus L. glycoside content, enhance color, fragrance and taste intensity, thereby significantly improving crocus sativus L. stigma yield and overall quality. In addition, the method significantly reduces crocus sativus L. corm rot disease occurrence through high-temperature treatment and the bacteriostatic effect of quicklime, and greatly improves comprehensive economic benefits of crocus sativus L. production.
Owner:HUZHOU CENT HOSPITAL

A system and method for propagating and improving the adaptability of native shrub seedlings suitable for high-altitude and arid regions.

This invention belongs to the field of native shrub seedling propagation technology, and discloses a system and method for propagating and improving the adaptability of native shrub seedlings suitable for high-altitude and arid regions. The system includes a data processing platform, acquisition equipment, and control equipment. The data processing platform establishes habitat and germplasm archives corresponding to the same germplasm batch and sequentially completes germplasm dormancy treatment, staged seedling management, root culture, mycotoxin-seedling symbiosis, stress substrate integration, and planting feedback. The method generates qualified germplasm records and germination initiation records based on germplasm status and dormancy type. Seedling stage status records serve as the basis for initiating the next stage. Root culture, mycotoxin inoculation, stress pre-adaptation, and substrate transition are performed based on the habitat information of the target restoration plot. After planting, survival rate, greening time, new shoot growth, and overwintering survival rate are recorded in the habitat and germplasm archive to correct parameters for subsequent batches.
Owner:HUADIAN TIBET ENERGY CO LTD BAYU BRANCH +1

Method for transforming and felling low-efficiency forest

The invention discloses a method for transforming and felling a low-efficiency forest, which relates to the field of forest cultivation and comprises the following steps of felling operation, stump treatment, fallen wood treatment and tending management. The felling operation is carried out innovatively by adopting at least one combination mode of a global felling method, a canopy felling method, a lower felling method, a crown diameter felling method and a target diameter-at-breast-height felling method according to forest stand characteristics and operation targets. The cutting strength adopts diameter at breast height; the cutting cycle is set according to the tree age, the wind damage risk, the tree growth height and the forest stand canopy density difference and is carried out in the tree dormancy period. According to the low-efficiency forest transformation felling method, through combined application of multiple targeted felling modes, accurate regulation and control of a forest stand structure are achieved; by adopting the diameter at breast height cutting strength index, the operation design process is simplified, and the operability is improved; measures such as pile cutting treatment and fallen timber on-site scientific stacking are combined, and multiple targets of water and soil conservation, soil fertility maintenance and ecological restoration are effectively considered.
Owner:JIANGSU ACAD OF FORESTRY

A method for controlling aphids on peach trees

ActiveCN120898673BFruit setHoneydew
The application discloses a peach tree aphid control method and belongs to the technical field of peach tree aphid control. The specific control method is as follows: (1) during the dormancy period of the peach tree, the bark of the peach tree is cleaned, and the branches and leaves with insect eggs are cleaned; before budding, a stone sulfur mixture is sprayed on the branch bud axilla, the bark crack and the soil surface under the tree; (2) during the flower bud period of the peach tree, an aphid killer is sprayed once during the red flower bud period; 6-7 days after the flower withering, a honeydew removing agent and an aphid killer are sprayed once; (3) during the growth period of the peach, 30-35 days after fruit setting, a honeydew removing agent and an aphid killer are sprayed once; (4) during the early dormancy period, a yellow plate coated with a sticky insect glue is hung on the sunny side of the peach tree.
Owner:NINGXIA ACADEMY OF AGRICULTURE AND FORESTRY SCIENCES INSTITUTE OF HORTICULTURE (NINGXIA FACILITY AGRICULTURE ENGINEERING TECHNOLOGY RESEARCH CENTER)

Method for promoting efficient germination of agriophyllum squarrosum seeds

The invention discloses a method for promoting efficient germination of agriophyllum squarrosum seeds, and relates to the technical field of plant tissue culture and genetic transformation. According to the invention, mechanical polishing treatment and ethanol disinfection are innovatively combined. The seed coat barrier is destroyed through physical polishing, so that the seed pollution rate is greatly reduced, the germination starting time is shortened to 24 hours, the germination period is compressed to be within 5 days, the synergistic inhibition effect of seed coat physical dormancy and microbial infection on seed germination is effectively broken through, and a key technical support is provided for establishing an agriophyllum squarrosum genetic transformation system.
Owner:NORTHWEST INST OF ECO ENVIRONMENT & RESOURCES CAS

A sea cucumber land-based breeding pond and a sea cucumber breeding method

ActiveCN117717032BRealize temperature regulationReduce the temperatureClimate change adaptationPisciculture and aquariaStichopusWater flow
This invention relates to a land-based sea cucumber culture pond and a method for cultivating sea cucumbers. The land-based culture pond comprises: several culture units, each including a net reef and pond water near the reef for sea cucumber cultivation; and several heat exchange pipes, each including a third pipe laid near the culture unit and cold water within the third pipe. The cold water flowing through the heat exchange pipes lowers the temperature of the pond water near the net reef by 2-5°C. This invention solves the problem of how to regulate the bottom water temperature of the land-based sea cucumber culture pond, thereby improving the survival rate of sea cucumbers during summer dormancy.
Owner:HEBEI ACAD OF MARINE & AQUATIC SCI

Efficient embryo germination culture method capable of breaking through deep dormancy characteristic of tilia amurensis

ActiveCN122030266APlant tissue cultureHorticulture methodsStratification (seeds)Vitamin C
The invention relates to the technical field of plant tissue culture and endangered tree seed breeding, in particular to an efficient embryo germination culture method for breaking through the deep dormancy characteristic of tilia amurensis, and aims at solving the problems that existing tilia amurensis seed breeding is low in germination rate, long in period, high in pollution rate and poor in embryo seedling quality. The method comprises the following steps: selecting robust tilia amurensis seed trees in specific areas such as 129 degrees, 19 '+ / -0.5 degrees and the like of the east longitude, and harvesting seeds with the water content of 45.25%-59.06% from August 29 to September 20; stratification is conducted at the low temperature of 4 DEG C for 20 days, 50 mg / L GA3 is added, soaking is conducted for 12 h, and pretreatment Precisely disinfecting with 75% alcohol and 2% sodium hypochlorite; cutting a 1-2mm notch on the seed coat, and then carrying out antioxidant treatment by using a vitamin C-humic acid solution; inoculating to an optimized MS culture medium containing hormones such as 0.1 mg / L 6-BA, carrying out dark culture at 22 DEG C for 5 days, and then carrying out 2000lx illumination culture at 24 DEG C; according to the method, deep dormancy is efficiently broken, the pollution rate is low, embryo seedlings are robust, and large-scale breeding and population recovery of tilia amurensis can be supported.
Owner:CHANGCHUN FORESTRY SCIENCE RESEARCH INSTITUTE

Ecological crab transport case and using method thereof

The invention relates to the technical field of aquatic living body transportation, in particular to a crab ecological transport case and a using method thereof.The transport case comprises a physical structure device and an environment regulation and control device, and the physical structure device comprises a bionic subdivision inner container, a dry-wet separation and condensate water management device and a stacking locking device; the environment regulation and control device comprises a temperature control device, an intelligent oxygenation and gas purification device, a bionic dormancy spraying device and a humidity control device. The method is suitable for the crabs which have the water-leaving metabolism regulation capacity and can be transported in a water-free keep-alive mode, near-physiological-state transportation of the crabs from the production place to the sales place is achieved, death and loss are reduced, and the vitality and commercial quality of the crabs are kept.
Owner:SHANDONG ACAD OF MARINE SCI (QINGDAO NAT MARINE SCI RES CENT)

Desertification-free cold storage low-temperature after-ripening method for plum-leaf crab seeds

The invention discloses a low-temperature after-ripening method for malus spectabilis seeds in a desertification-free refrigeration house, and relates to the field of low-temperature after-ripening method.The low-temperature after-ripening method comprises the following steps that S1, multi-dimensional fruit grading and targeted repairing are conducted, directional pretreatment and targeted repair are carried out on fruits with different maturity degrees and damage grades; s2, adaptive stepped pre-cooling: according to the maturity and damage grade of the fruits, different pre-cooling strategies are adapted; s3, performing zoning and layering temperature-changing after-ripening; and S4, carrying out integrated connection treatment. According to the method, fruit maturity and micro-damage grading is completed within 2 h after harvesting, directional pretreatment and targeted repair are matched, the situation that dormancy of low-maturity fruits is not thoroughly removed is avoided, the hidden danger of mold breeding caused by damaged fruits is eliminated, meanwhile, by combining after-ripening environment humidity and gas concentration regulation and control, reproduction of penicillium and fusarium is reduced, the seed mildew rate is reduced, and the seed quality is improved. The after-ripening uniformity of various fruits is improved.
Owner:TONGCHUAN ZHAOJIN HAITANG ECOLOGICAL IND CO LTD

Malania oleifera seed germination accelerating method and application thereof

PendingCN120982259ABiocidePlant growth regulatorsStratification (seeds)Gibberellin
The invention discloses a malania oleifera seed germination accelerating method and application thereof, and the method comprises the following steps: (1) applying mechanical force to malania oleifera seeds, so as to generate cracks on seed shells of malania oleifera to conduct the inner space of the seed shells and the outer part of the seed shells; the crack longitudinally extends on the seed shell and penetrates through the top of the seed shell; (2) soaking the seeds treated in the step (1) in an aqueous solution containing a dormancy breaking agent; the dormancy relieving agent comprises gibberellin and a cytokinin analogue; and (3) sowing the seeds treated in the step (2) in a breathable and water-containing culture medium to perform stratification and germination acceleration, and performing temperature control and regular irrigation in the period until the seeds germinate. According to the application, the method is applied to germination acceleration of malania oleifera seeds collected from wild malania oleifera plants in Guangxi Zhuang Autonomous Region and / or Yunnan.
Owner:INSTITUTE OF CHINESE MATERIA MEDICA CHINA ACADEMY OF CHINESE MEDICAL SCIENCES +1

Ice temperature storage method for prolonging storage and fresh-keeping period of figs

The invention relates to an ice-temperature storage method for prolonging the storage and preservation period of figs, and belongs to the technical field of fruit preservation. The method aims at solving the problems that figs rot after being picked at normal temperature for 1 day, are easy to frostbite and wilt after being stored at low temperature and depend on chemical preservatives in the prior art. The invention provides a green physical fresh-keeping scheme which comprises the following steps: selecting 70-80% mature figs, and pre-cooling at 4 + / -2 DEG C for 4-6 hours to remove field heat; randomly sampling and measuring a freezing point, and then setting a storage temperature; placing the pre-cooled fruits in a buffer temperature zone with the temperature difference being greater than or equal to 2 DEG C above the freezing point for treatment for 4-6 hours, and slowly reducing the temperature of the fruits to 0-2 DEG C; and finally, storing for a long time at the temperature of-2 DEG C to-1 DEG C below the freezing point and higher than the freezing point under the humidity of 90 + / -5%. According to the method, the figs enter deep dormancy through precise ice temperature control, maturation and senescence are remarkably delayed, the storage period can be prolonged to 6 days or above, the hardness is kept to be larger than or equal to 9N, the rotting rate is lower than 5%, and the method is suitable for commercialized preservation of the picked figs.
Owner:INST OF AGRO FOOD SCI & TECH CHINESE ACADEMY OF AGRI SCI

Seed treatment nutrition powder for efficient seedling raising of direct sowing of rhizoma bletillae

PendingCN121795456Aisolation inhibitionconducive to survivalBiocidePlant growth regulatorsBiotechnologyBletilla striata
The invention discloses rhizoma bletillae direct seeding efficient seedling culture seed treatment nutrition powder, and relates to the technical field of traditional Chinese medicine seedling culture. The nutrition powder provided by the invention comprises a symbiotic fungus-nutrition synergistic protection system, a plant source dormancy breaking-stress resistance composite system, a moss inhibition-nutrient activation integrated component and a basic nutrition matrix. According to the symbiotic fungus-nutrition synergistic protection system, the activity and functions of fungi are guaranteed through double-fungus mixing, composite carrier embedding and synergist compounding; according to the plant source system, various plant extracts are compounded to replace chemical hormones, and dormancy breaking and stress resistance enhancing are both considered; the integrated component realizes synchronization of moss inhibition and nutrient activation by virtue of carrier loading; the basic substrate is subjected to starch acetylation modification and glucose proportioning, nutritional requirements of different growth stages are met, all systems are subjected to low-temperature spray granulation to form a final product, the direct sowing seedling raising efficiency and seedling quality of rhizoma bletillae can be improved, and the method is suitable for large-scale green seedling raising.
Owner:LONGNAN INST OF AGRI SCI