150 results about "Dormancy" patented technology

The invention belongs to the technical field of remote sensing, and discloses a wolfberry planting distribution high-precision remote sensing monitoring method based on multi-temporal fusion and spectral feature optimization. According to the method, a multi-temporal and multi-spectral satellite image is used as a data source, and preprocessing and multi-temporal image fusion are firstly carried out; the method comprises the following core steps: establishing a time sequence characteristic curve according to a unique phenological period (such as bare soil characteristics in a dormancy period and high vegetation coverage in a rapid growth period) of wolfberry; in a spectral domain, screening out a characteristic spectrum dimension combination with the highest discrimination degree between the wolfberry and other crops through a characteristic wave band optimization algorithm (such as vegetation index difference degree and red edge characteristics); and in combination with an object-oriented classification or deep learning classification model, constructing a space-time coupling classifier, and performing high-precision extraction and distribution mapping on the Chinese wolfberry planting region. The method can effectively solve the problem of confusion classification of Chinese wolfberry and similar ground features (such as other shrubs and orchards), and realizes rapid and accurate monitoring of Chinese wolfberry planting area and spatial distribution.

The invention discloses an application and a method for knocking out a poplar LUX1 gene in delaying growth stop or dormancy time under short day, and the poplar LUX1 gene is identified and obtained through amino acid sequence comparison with arabidopsis thaliana LUX. The poplar LUX1 gene is a protein for coding an amino acid sequence as shown in SEQ ID No.1. The LUX1 gene is knocked out through a CRISPR / Cas9 editing system, and the time that the poplar enters the dormancy period due to short sunlight in autumn is delayed, so that the poplar germplasm with long growth period and high biomass is obtained. After the LUX1 gene is knocked out, terminal bud growth stop and stem cambium activity reduction in the seasonal growth process of the poplar can be delayed, so that wood development is promoted. Deferring of the dormancy time under the autumn short-day condition has a great application prospect for prolonging the growth period of the trees so as to improve photosynthetic carbon sequestration and biomass accumulation.

The invention relates to the technical field of facility agriculture, in particular to a green cultivation method for greenhouse water culture of Chinese chives. Through integration of intelligent greenhouse facilities, staged nutrient solution regulation and control, physical-biological-chemical synergistic disease and insect pest prevention and control and an annual production management technology, the problems of continuous cropping obstacles, high pesticide residues, winter dormancy and the like existing in traditional soil culture of Chinese chives are solved. According to the method, annual production of Chinese chives is achieved, the harvesting period of each crop is shortened to 20-30 days, compared with traditional soil culture, the annual yield is increased by 40% or above, the pesticide use amount is reduced by 95%, the pesticide residue percent of pass is 100%, and the product is free of heavy metal pollution, meets the green food standard and is suitable for green transformation and popularization of facility agriculture.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for early identification of the cold resistance of an actinidia chinensis tree body in the dormancy period, and the SNP molecular marker is located at the Chr69297479 position on the No.6 chromosome of the actinidia chinensis and is closely associated with the cold-resistant phenotype median lethal temperature (LT50) of the actinidia chinensis. The method can be used for identifying the tree body dormancy period cold resistance of natural population and hybrid filial generation of actinidia chinensis. The homozygous genotype (CC) of the SNP locus corresponds to a high-cold-resistance individual in the dormancy period of an actinidia chinensis tree body, and the heterozygous genotype (TC) of the SNP locus corresponds to a low-cold-resistance individual. The cold resistance prediction accuracy in the F1-generation hybrid population reaches 94.94%, and the prediction accuracy in the natural population reaches 96.15%. The molecular marker detection method disclosed by the invention is convenient and rapid, has good specificity and high accuracy, is not influenced by climate, environment and the like, can rapidly and effectively evaluate the cold resistance of natural resources and hybrid filial generations of the actinidia chinensis, and provides powerful technical support for breeding cold-resistant varieties of the actinidia chinensis.

The invention relates to the technical field of flower flowering phase regulation and control, and discloses a regulator and a method for promoting secondary flowering of crape myrtle, the regulator comprises 50 parts by weight of benzylaminopurine, 4.6-5 parts by weight of compound sodium nitrophenolate and more than or equal to 100 parts by weight of an amino acid composition; or 18 parts by weight of benzylaminopurine, 18 parts by weight of gibberellin, 4.6-5 parts by weight of compound sodium nitrophenolate and more than or equal to 100 parts by weight of an amino acid composition. The regulator provided by the invention comprehensively acts on multiple aspects of cell division, dormancy breaking, hormone regulation, nutrition supply, stress resistance enhancement and the like to jointly promote secondary blooming of the crape myrtle, increase the blooming amount and prolong the blooming period, and by virtue of multiple aspects of effects of pruning, fertilization, insect killing, plant growth regulator regulation and the like, the yield of the crape myrtle is increased. Secondary blooming of the crape myrtle is promoted, the blooming rate, the blooming duration and the blooming amount of secondary blooming are increased, and the growth vigor of the crape myrtle in the next year is not affected.

The invention discloses an SSR molecular marker primer related to leek dormancy as well as a detection method and application of the SSR molecular marker primer. Aiming at the problems that the traditional dormancy character identification of Chinese chives depends on field morphological observation as long as 4 months, the efficiency is low and special molecular markers are lacked, seven pairs of specific SSR primers (SSR005 / 170 / 249 / 325 / 423 / 478 / 610) are developed based on cross-species genome re-sequencing (taking green Chinese onion as a reference genome, and the comparison rate is 18.76%-18.89%) for the first time. The method comprises the following steps: establishing a matched detection system: rapidly extracting DNA (deoxyribonucleic acid) by a CTAB (cetyltrimethyl ammonium bromide) method, optimizing a PCR (polymerase chain reaction) system (20 mu L of DNA containing a template of 30 ng, 0.4 U of Taq enzyme, 0.2 mu M of primer, 2.0 mM of Mg < 2 + >, 0.2 mM of dNTPs, and annealing at 57 DEG C), and performing 8% polyacrylamide gel electrophoresis combined with silver staining development, thereby accurately identifying the dormancy character within 7 days. For example, the SSR423 primer has non-differentiated non-dormancy / dormancy germplasm at 177bp, and the coincidence rate of the detection result of 52 germplasm (such as dormancy germplasm 21-1 and non-dormancy germplasm 22-7) and the field phenotype reaches 100%.

The invention provides a peach tree artificial dormancy breaking and early forcing cultivation method, and relates to the field of agricultural cultivation. The artificial dormancy-breaking and early-promoting cultivation method for the peach trees comprises the following specific cultivation methods: step 1, selecting high-quality peach tree seedlings, maintaining a low-temperature environment of 4 DEG C or below in August, enabling the accumulated dormancy time to reach 40-50 days, and spraying 5% seaweed extract at the top ends of young sprouts to promote bud activation; 2, in the middle ten days of August, leaves are removed, water is controlled, growth is forcibly terminated, a 5% humic acid solution is sprayed after leaf picking to enhance stress resistance, a sunshade net with the shading rate of 50% is erected at the beginning of September, and the daytime temperature is reduced by 3-5 DEG C; 3, a daytime covering and night uncovering method is adopted, wherein a heat preservation quilt and a straw mat are covered in the daytime for blocking and heating. Under the condition that the climate becomes warm and the requirement for advanced cultivation of the peach trees cannot be met only through the day and night temperature difference, physical cooling is conducted on the tree disks through a physical method, resources can be reused through crushed ice, and the problem that the cost of the physical method is high is solved.

This invention discloses the application of knocking down or knocking out the OsABA8ox2 protein or its encoding gene in improving the panicle germination resistance of rice seeds, belonging to the fields of biotechnology and plant genetic engineering technology. OsABA8ox2 The amino acid sequence of the protein is shown in SEQ ID NO.2, and the nucleotide sequence of the gene is shown in SEQ ID NO.1. This invention utilizes CRISPR / Cas9 technology to directionally edit the target site sequence in this gene, as shown in SEQ ID NO.3, successfully obtaining rice nucleotide sequences as shown in SEQ ID NO.8, SEQ ID NO.9, and SEQ ID NO.10. OsABA8ox2 The mutant, to a certain extent, enhances the dormancy of rice seeds, effectively reducing the germination rate of rice panicles. The gene resources and gene editing technology provided by this invention offer an important technical approach for rapidly improving or creating rice varieties with significantly enhanced panicle germination resistance in agricultural production, demonstrating significant application value in production practice.

The invention belongs to the technical field of edible fungus cultivation, and particularly relates to a method for improving the production efficiency of gastrodia elata f.glauca. The whole cultivation period of the gastrodia elata f.glauca can be effectively shortened, and the cultivation period can be shortened; according to the method, the bacteria retreating phenomenon of the armillaria mellea can be effectively reduced, compared with a traditional cultivation fungus bed, the armillaria mellea is only punched and is not completely opened, and even if part of the armillaria mellea dies in the cultivation process, new armillaria mellea can germinate in the bag to promote growth of gastrodia elata and prevent bacteria retreating; the high-nutrition armillaria mellea stick is prepared, and the formula nutrition of the armillaria mellea stick is suitable for supplementing organic nitrogen lacking in wood and can promote growth of armillaria mellea; when gastrodia elata f.glauca is planted, the biggest risk point is that armillaria mellea cannot be inoculated with wood and gastrodia elata seeds, the gastrodia elata seed dormancy breaking nutrient solution and the wood pretreatment nutrient solution are adopted, the risk can be effectively avoided, and the overall planting risk is reduced.

The present invention discloses the use of the MYC2 gene of Pansy as a positive regulatory factor in promoting the release of plant ecodormancy, relating to the field of biotechnology. The present invention, for the first time, demonstrates the important role of the MYC2 gene in releasing ecodormancy in Pansy by transiently overexpressing a homologous gene. The invention also confirms that the gene is localized in the cell nucleus and that its expression increases with the release of ecodormancy. Overexpression of the MYC2 gene in Pansy promotes the release of plant ecodormancy and germination.

The invention discloses a method for breaking the dormancy period of paris polyphylla seeds. The method comprises the steps of S1, seed pretreatment, wherein the paris polyphylla seeds are washed and disinfected; s2, soaking treatment: soaking the pretreated paris polyphylla seeds in a gibberellin solution in a dark environment at 22 DEG C; s3, germination accelerating treatment: placing the soaked seeds in a sterile culture dish, alternately placing the seeds in a dark low-temperature environment, and repeatedly accelerating germination of the seeds; S4, rapid propagation: transferring the germinated paris polyphylla seeds subjected to germination accelerating to a basic culture medium, adding NAA and 6BA into the basic culture medium, and meanwhile, cooperating with a light environment, so as to obtain sterile plants. According to the method for breaking the dormancy period of the paris polyphylla seeds, the germination rate is increased, the germination rate of the paris polyphylla seeds is remarkably increased through a gibberellin treatment and cold treatment combined method, and the technical problem that the germination rate of the paris polyphylla seeds is low is solved; the planting period is shortened; the rapid propagation of the paris polyphylla is realized.

The invention provides a greenhouse grape early-maturing-promoting cultivation method which comprises the following cultivation steps that S1, a gobi land parcel is excavated by 1.2 m to form a semi-underground structure, and a wall body is formed by mixing and ramming local gravel, straw and a cement curing agent. Efficient utilization of photo-thermal resources and facility stability are achieved under extreme climate, a degradable root limiting container and a biochar improved matrix are adopted, a root system micro-domain environment is improved, soil degradation is avoided, a multi-parameter sensing and photo-temperature coupling model can accurately regulate and control the dormancy period and the germination process, and the method is suitable for large-scale popularization and application. Meanwhile, active defense of disasters such as sand and dust and cold waves is achieved through an adversity response mechanism, the fruit quality and production benefits are remarkably improved, and a sustainable cultivation scheme with high environmental adaptability and high resource intensification degree is provided for the arid gobi area facility grape industry.

The invention provides a rapid dormancy breaking method for sand whip seeds and dormancy breaking sand whip seeds, and relates to the technical field of plant seed treatment.The method comprises the following steps that 1, physical initiation treatment is conducted, specifically, the disinfected sand whip seeds are placed in warm water at the temperature of 45-55 DEG C to be soaked for 10-14 h; (2) chemical initiation treatment: soaking the seeds treated in the step (1) in a gibberellin solution with the mass concentration of 0.03%-0.07% for 20-28 hours; (3) biological seed dressing treatment: carrying out seed dressing on the seeds treated in the step (2) and a trichoderma harzianum fungicide, wherein the mass of the fungicide is 0.05-0.15% of the mass of the seeds; according to the method, the dormancy mechanism of the sand penis seeds can be effectively broken, germination of the seeds is promoted, the germination rate of the sand penis seeds treated by the optimized method can reach 87.00% on the 21st day, the germination synchronism of the seeds can be higher, and the problem of irregular natural germination is solved.

The invention provides a non-dormancy direct germination technology for corn seeds, which can enable immature corn seed embryos to directly germinate and grow into plants without induction treatment of an MS culture medium, disinfection pretreatment of corn ears and the like. The method can accelerate seed germination and effectively shorten the corn growth cycle. According to the non-dormancy direct germination technology for the corn seeds, the germination rate can reach 97%, the seedling rate can reach 97%, and technical support is provided for genetic breeding or physiological research.

The application discloses a SSR molecular marker primer related to Chinese chives dormancy and a detection method and application thereof. In view of the problems that traditional Chinese chives dormancy trait identification relies on field morphological observation for 4 months, is inefficient and lacks special molecular markers, 7 specific SSR primers (SSR005 / 170 / 249 / 325 / 423 / 478 / 610) are developed based on cross-species genome resequencing (taking Chinese onion as a reference genome, and the alignment rate is 18.76%-18.89%) for the first time. Through the establishment of a matching detection system: CTAB method for rapid DNA extraction, optimization of the PCR system (20 muL contains 30 ng of template DNA, 0.4 U of Taq enzyme, 0.2 muM of primer, 0.2 mM of Mg 2+ 2.0 mM of dNTPs, 57 DEG C annealing), 8% polyacrylamide gel electrophoresis combined with silver staining development, the dormancy trait can be accurately identified within 7 days. For example, the SSR423 primer has no band at 177 bp, and the non-dormant / dormant germplasm cannot be distinguished. The detection results of 52 germplasms (such as dormancy germplasm 21-1 and non-dormancy germplasm 22-7) are consistent with the field phenotypes at a rate of 100%.

The invention belongs to the technical field of economic forest cultivation, and particularly relates to a method for improving the germination rate of hovenia acerba seeds and the transplanting survival rate of seedlings. The method comprises the following steps: (1) peeling hovenia acerba seeds; (2) floating and screening in a saturated saline solution; (3) seed disinfection; (4) chemical wall breaking; (5) breaking dormancy by gibberellin; (6) promoting germination by melatonin; (7) seedbed preparation; (8) seedbed sowing; (9) sand covering and film covering; (10) seedling exercising; (11) field transplanting. By means of the method, the germination time of the hovenia acerba seeds can be advanced by 11-14 d, the germination period is shortened by 15-43 d, the germination rate is increased to 80.00% or above, the transplanting survival rate is increased, and time and labor cost are reduced.

The present application belongs to the field of live transportation of aquatic products, and relates to a method for live transportation of prawns without water. The core of the method is to use a staged treatment strategy and the synergistic effect of dormancy induction liquid and moisture supply liquid. First, the prawns are adapted to the environment by fasting and gradient cooling, then they are soaked in pre-cooled dormancy induction liquid to enter a pseudo-death dormancy state and reduce metabolism. Then, the moisture supply liquid soaked sponge is used as a transportation medium, which can form a protective film on the surface of the prawn body to effectively prevent water loss and provide energy. During transportation, the prawns treated with the moisture supply liquid are placed in a sealed box filled with pure oxygen together with the moist sponge, and the prawns are transported without water at low temperature. After the transportation is completed, the prawns are placed in warm water to quickly recover. The prawn live transportation method without water prolongs the survival time of prawns in a water-free environment, improves the transportation efficiency, is simple to operate, and is suitable for large-scale long-distance transportation of prawns.

The invention discloses a method for awakening dormancy of phellinus igniarius sporocarp and quickly recovering growth, which comprises the following steps: pretreatment: placing dormant phellinus igniarius sporocarp in an environment with the temperature of 18-20 DEG C, and spraying water on the surface of the phellinus igniarius sporocarp to make the water content of the surface of the phellinus igniarius sporocarp reach 65-75%; a sterile scalpel is used for cutting an aged tissue layer on the surface of the pretreated phellinus igniarius sporocarp, so that a fresh tissue wound surface is exposed outside, the cutting thickness is 0.5 mm-1 mm, and the cutting area accounts for 50%-90% of the surface area of a non-growing area of the phellinus igniarius sporocarp; covering with a film: covering the cut part of the phellinus igniarius sporocarp with a dark film, and standing for 3-6 days; the deep-color film is removed, and cultivation management is conducted on the phellinus igniarius sporocarp according to conventional cultivation conditions. According to the method, the awakening period of phellinus igniarius is shortened, the recovery growth rate and yield of the phellinus igniarius sporocarp are increased, and the infection rate of infectious microbes is reduced.

The invention relates to the technical field of agricultural biology, in particular to a method for increasing the germination rate of hemerocallis citrina L. seeds, the method for remarkably increasing the germination rate of the hemerocallis citrina L. seeds through combined treatment of gibberellin GA3 and hydrogen peroxide H2O2 is easy and convenient to operate and low in cost, the physiological dormancy of the seeds is broken through gibberellin, embryo cell division is promoted, and the germination rate of the seeds is increased. Meanwhile, hydrogen peroxide is used for moderately oxidizing seed coats, permeability is improved, active oxygen signal molecules are provided for activating germination related enzyme systems, the hydrogen peroxide and the active oxygen signal molecules generate a synergistic interaction effect through a dormancy breaking-permeation promoting-activation triple mechanism, the germination rate is increased in a breakthrough mode, and the germination period is remarkably shortened.

The invention discloses a simple and efficient method for breeding seedling potatoes by using potato seeds, through the links of seed treatment optimization, seedbed preparation, sowing key points, post-seedling management, harvesting key points, storage measures and the like, the germination rate and the plant rate of the potato seeds are remarkably improved, disease propagation is reduced, the production cost is reduced, and the method is suitable for large-scale popularization and application. And meanwhile, the quality of the seedling potatoes is improved. It is guaranteed that the seedling potatoes can live the dormancy period healthily, and the soil ecological environment is guaranteed.

The present application relates to the technical field of plant tissue culture, and specifically discloses a method for establishing a tissue culture sterile system of peach rootstock dormancy material, which comprises the following steps: S1, selecting one-year-old branches with leaf buds in the dormancy period, removing flower buds and cleaning; S2, performing low-temperature treatment on the branches, then performing water culture and bud breaking to obtain sprouting branches; S3, performing surface sterilization treatment on the sprouting branches, and then washing with sterile water; S4, peeling the sprouting buds from the branches under sterile conditions, removing the external wrapping tissues of the bud bodies, and exposing the internal tender tissues; S5, performing secondary sterilization treatment on the exposed tender tissues, and then washing with sterile water; and S6, inoculating the bud bodies subjected to the secondary sterilization treatment into a culture medium for culture, and establishing a sterile system; according to the above method, in the process of establishing the sterile system of Zhongtao Kangzhen No. 1 material, the sterilization survival rate of 100 bud heads is as high as 80% or more, and the work efficiency and the utilization efficiency of the material are greatly improved.

Disclosed is a new variety of Prunus persica named ‘TRH4D144WN’, which requires approximately 500 chilling units of dormancy, produces an acid-sweet, white-fleshed firm clingstone nectarine with a 70-90% medium to dark pink blush that matures in the early mid-season.

The invention belongs to the technical field of agricultural planting, and provides a migrant bird type fruit tree aeroponic cultivation method and device.The method comprises the steps that a sapling to be migrated is cultivated in the north to form a tree body with the flower bud differentiation capacity; wrapping the root system of the tree body and then putting the tree body into a refrigeration house, so that the tree body performs cold storage and dormancy; transporting the tree body after cold storage and dormancy from the north to the south; and planting the dormant tree bodies in the southern region, so that the tree bodies blossom and bear fruits. The method can be suitable for anti-season, super-early-maturing or peak-shifting production scenes of northern deciduous fruit trees which can break dormancy only through cold stimulation at a low temperature of a certain hours in winter.

The invention relates to the technical field of narcissus cultivation, and discloses a narcissus full-year accurate flowering phase control method and system based on environmental factor control, and the method comprises the following specific steps: narcissus dormancy period pretreatment: selecting narcissus seedballs, and carrying out wrapping treatment on the narcissus seedballs; storing the narcissus in a dormancy period: storing the wrapped narcissus seedballs in a freezer, and regularly ventilating; daffodil wake-up treatment and cultivation: taking daffodil seedballs in a dormancy period, transferring the daffodil seedballs into a sunlight glass house, and cultivating the daffodil seedballs by using a culture medium; according to the daffodil full-year accurate flowering phase control method and system based on environmental factor control, daffodil seedballs are selected and subjected to dormancy treatment, when daffodil needs to bloom in a specific period, the daffodil seedballs subjected to dormancy treatment are awakened, and multi-dimensional environmental regulation and control such as temperature, illumination and humidity are fused, so that the flowering phase of daffodil is accurately controlled. Precise control over the flowering period of narcissus can be achieved, and then the requirements of different flowering periods of narcissus are met.

The utility model discloses soil remediation equipment utilizing microorganisms, and belongs to the field of soil remediation. Comprising a rotary cultivator used for turning up soil; the microorganism cultivation tank is used for cultivating microorganisms; the feeding assembly is arranged on the rotary cultivator and used for feeding the microorganisms in the microorganism cultivation tank into soil; the flow switch is arranged on the microorganism cultivation tank, an outlet of the flow switch is connected with a feeding pipe, and the other end of the feeding pipe is connected with the feeding assembly; an inner cavity of the microorganism cultivation tank comprises a dormancy area cavity and a cultivation area cavity, the dormancy area cavity is communicated with the bottom of the cultivation area cavity through a pipeline, dormancy gas is arranged in the dormancy area, a growth lamp strip is arranged in the cultivation area, and a microbial agent inlet communicated with the cultivation area cavity is formed in the microorganism cultivation tank. The soil remediation equipment has the beneficial effect that the soil remediation equipment utilizing the microorganisms is provided.

The invention discloses application of a fringed iris IjFLCL1 gene to regulation and control of fringed iris growth and ecological dormancy conversion. When the fringed iris needs to be kept in an ecological dormancy state, the IjFLCL1 gene is silenced or knocked out; when the fringed iris needs to be inhibited from entering the ecological dormancy state, the IjFLCL1 gene is subjected to overexpression; the nucleotide sequence of a protein coding region of the fringed iris IjFLCL1 gene is as shown in SEQ ID No. 1. According to the invention, a homologous gene function verification method is adopted in a plant for the first time, and it is proved that silence or knockout of the IjFLCL1 gene can maintain fringed fringed iris in an ecological dormancy state. The gene has the highest expression quantity in the spring growth period and the lowest expression quantity in the dormancy induction period. Silence or knockout of the gene can delay removal of ecological dormancy of fringed iris and help plants avoid adverse effects caused by early spring climate fluctuation, so that safety and economic benefits in the cultivation process are improved.

The utility model belongs to the technical field of live fish dormancy transportation, and discloses a temperature control type live fish dormancy transportation device which comprises a transportation vehicle hopper, a water storage pool is fixedly installed on the inner wall of the bottom of the transportation vehicle hopper, a sealing baffle is arranged on the top of the water storage pool, and a plurality of fixing plates are fixedly installed on the left side and the right side of the water storage pool. Mounting frames are fixedly mounted at the four corners of the top of the water storage tank, through the arranged collecting assembly, when live fishes are transported, the live fishes can be placed above a filter plate, excrement of the live fishes can fall to the bottom of the water storage tank through the filter plate, and after transportation is completed, a motor can be controlled to drive a take-up roller to rotate; and after the take-up rollers rotate, the take-up rollers on the two sides can rotate through belt wheels and synchronous belts, after the take-up rollers rotate, a lifting frame can be driven to ascend through a steel wire rope, workers can conveniently take live fishes, and excrement on the inner side can be conveniently cleaned after a filter plate is detached.

The invention provides a polygonatum kingianum tissue culture medium and application thereof, and belongs to the technical field of plant tissue culture. The polygonatum kingianum tissue culture medium comprises a seed induction medium, a cluster bud induction medium, a proliferation medium and a rooting medium. The seed induction culture medium can promote seed germination and expansion; the cluster bud induction culture medium can improve the activity of the explant, so that the explant forms a large number of cluster buds in a relatively short time; the proliferation culture medium can induce and generate a large number of healthy buds, and the expansion condition of the rhizomes can be obvious; the rooting culture medium can effectively improve the rooting rate and promote the root system to grow well and uniformly. The polygonatum kingianum tissue culture medium provided by the invention breaks the dormancy mechanism of polygonatum kingianum seeds, shortens the germination time of the polygonatum kingianum seeds, can obtain a large number of cluster buds in a short time, further obtains seedlings, and is beneficial to tissue culture and rapid propagation of the polygonatum kingianum seeds.

The invention discloses a leek SSR-PCR reaction system and application thereof, and solves the technical bottlenecks of long period (more than or equal to 4 months), high error rate (more than or equal to 15%) and low flux (less than 10 parts per day) caused by the fact that traditional dormancy character identification depends on field phenotype observation. The system takes a precisely optimized 20 [mu] L reaction system as a core, comprises 30 ng of template DNA, 0.4 U of Taq enzyme, 0.2 [mu] M of primer, 2.0 mM of Mg < 2 + > and 0.2 mM of dNTPs, and realizes stable amplification by combining with a 57 DEG C annealing temperature program; seven SSR molecular markers linked with dormancy characters are synchronously developed, and the SSR423 marker judges dormancy phenotypes through 177bp strip deletion. During application, DNA is extracted through a CTAB method, after amplification of the system, germplasm typing can be completed within 8 hours through polyacrylamide gel electrophoresis detection, 52 germplasm verification shows that the consistency with field phenotypes reaches 100%, and the flux is increased to be larger than 200 parts per day. The technology provides an efficient molecular tool for breeding of leek dormancy-resistant varieties, and annual balanced supply of the market is guaranteed.

This invention relates to the field of endangered aquatic biological resource protection and aquaculture technology, specifically to an integrated method for the harvesting, transportation, and domestication of the wavy-lipped barb, comprising the following steps: S1, behavioral adaptation-based nocturnal dive-fishing and decompression: during the wavy-lipped barb's nocturnal dormancy period, the target fish are gently induced into a pressure-regulating container; the fish are then safely transported to the water surface; S2, multi-stage live-preservation transportation: the fish treated in S1 are first transported via a live-water vessel for rehabilitation at sea, and then smoothly transferred via a live-water truck on land; S3, gradient-adaptive artificial domestication; S4, live germplasm resource preservation. This invention ensures that wild wavy-lipped barbs receive physiological protection at each transfer point, maximizing the maintenance of their internal environmental homeostasis, thereby increasing the overall survival rate to nearly 100% and providing high-quality, healthy parent fish for subsequent artificial breeding.