476 results about "Abortion" patented technology

Porcine circovirus-2 (PCV-2) is a recently identified agent wherein the potential spectrum of PCV-2-associated disease has been expanded by evidence of vertical and sexual transmission and associated reproductive failure in swine populations. PCV-2 was isolated from a litter of aborted piglets from a farm experiencing late term abortions and stillbirths. Severe, diffuse myocarditis was present in one piglet associated with extensive immunohistochemical staining for PCV-2 antigen. Variable amounts of PCV-2 antigen were also present in liver, lung and kidney of multiple fetuses. Inoculation of female pigs with a composition including an immunogen from PCV-2 or an epitope of interest from such an immunogen or with a vector expressing such an immunogen or epitope of interest prior to breeding, such as within the first five weeks of life, or prior to the perinatal period, or repeatedly over a lifetime, or during pregnancy, such as between the 6th and 8th and / or the 10th and 13th weeks of gestation, can prevent myocarditis, abortion and intrauterine infection associated with porcine circovirus-2. In addition, innoculation of male and / or female pigs with the aforementioned compositions can be carried out to prevent transmission of PCV-2 from male to female (or vice versa) during mating. Thus, the invention involves methods and compositions for preventing myocarditis, abortion and intrauterine infection associated with porcine circovirus-2.

Porcine circovirus-2 (PCV-2) is a recently identified agent wherein the potential spectrum of PCV-2-associated disease has been expanded by evidence of vertical and sexual transmission and associated reproductive failure in swine populations. PCV-2 was isolated from a litter of aborted piglets from a farm experiencing late term abortions and stillbirths. Severe, diffuse myocarditis was present in one piglet associated with extensive immunohistochemical staining for PCV-2 antigen. Variable amounts of PCV-2 antigen were also present in liver, lung and kidney of multiple fetuses. Inoculation of female pigs with a composition including an immunogen from PCV-2 or an epitope of interest from such an immunogen or with a vector expressing such an immunogen or epitope of interest prior to breeding, such as within the first five weeks of life, or prior to the perinatal period, or repeatedly over a lifetime, or during pregnancy, such as between the 6and 8and / or the 10and 13weeks of gestation, can lower viral titer, prevent myocarditis, abortion and intrauterine infection associated with porcine circovirus-2. In addition, innoculation of male and / or female pigs with the aforementioned compositions can be carried out to prevent transmission of PCV-2 from male to female (or vice versa) during mating. Thus, the invention involves methods and compositions for preventing myocarditis, abortion and intrauterine infection associated with porcine circovirus-2.

The present invention relates to the use of an immunogenic composition that comprises a porcine circovirus type 2 (PCV2) antigen for treatment of several clinical manifestations (diseases). Preferably, the clinical manifestations are associated with a PCV2 infection. Preferably, they include lymphadenopathy, lymphoid depletion and / or multinucleated / giant histiocytes. Moreover, the clinical symptoms include lymphadenopathy in combination with one or a multiple of the following symptoms in pigs: (1) interstitial pneumonia with interlobular edema, (2) cutaneous pallor or icterus, (3) mottled atrophic livers, (4) gastric ulcers, (5) nephritis and (6) reproductive disorders, e.g. abortion, stillbirths, mummies, etc. Furthermore the clinical symptoms include Pia like lesions, normally known to be associated with Lawsonia intracellularis infections.

Provided is a method for reducing viral load of porcine circovirus type 2 (PCV-2) in a pig by inducing an immune response against PCV-2 through the administration of an immunogenic composition comprising a PCV-2 antigen. A preferred antigen is a vector containing a PCV-2 nucleotide sequence. In a particularly preferred embodiment, the PCV-2 nucleotide sequence is ORF4, ORF13, or ORF4 and ORF13. In some embodiments, the immunogenic composition includes one or more additional pig pathogens.

By exploiting an early release facility that may be provided by certain transactional memory designs, we allow for transaction software constructs that wait on removal (or satisfaction) of a condition that would otherwise result in transaction abort. Absent exploitation of such a such a facility, the act of checking the condition would typically introduce a corresponding location into the read set of the transaction, and a subsequent modification of that location that removed (or satisfied) the condition, would result in abortion of the blocked transaction. By exploiting an early release facility such as described herein, a transaction may release the location (or locations) corresponding the condition, retry, and once the transient condition is removed (or satisfied), complete and commit. In this way, computation effort may be conserved while still employing a conceptually simple and convenient coordination facility.

The invention relates to a Chinese medicinal composition for treating rheumatism. Diagnosis and treatment based on an overall analysis of the illness and the patient's condition is performed on the basis of the basic theory of traditional Chinese medical science, so the Chinese medicinal composition is particularly suitable for treating diseases such as rheumatic pyretic arthralgia, wind-cold damp arthralgia, blockage of phlegm and blood stasis and the like. In addition, the Chinese medicinal composition is also used for treating amenorrhea and fetal abortion (terminal pregnancy). The invention also relates to various pharmaceutically-acceptable formulations of the Chinese medicinal composition and particularly a minium black plaster, a preparation and a preparation method of the Chinese medicinal composition. The Chinese medicinal composition has the characteristics that: the diagnosis and treatment based on an overall analysis of the illness and the patient's condition is performed, the pertinence is high, the convenience is provided for patients who suffer from the rheumatism and the curative effect is definite; and thus, the Chinese medicinal composition is a comprehensive Chinese traditional treatment formula.

This invention provides methods for the amelioration, termination and / or abortion of a seizure by the intrapulmonary administration of a benzodiazepine, for example, midazolam.

The invention provides an application of application of plant alpha-diastase in pollen abortion, and belongs to the technical field of genetic engineering. Through introducing polynucleotide of the plant alpha-diastase, recombinant vector and recombinant vector are structured; a pollen specific promoter drives the plant alpha-diastase to degrade starch in advance during the pollen developmental phase; the pollen sprouting energy cannot be provided, and the pollen sprouting is contained, thus abortion is generated; the transgenetic seedling of transgene pollen abortion is obtained. The plant alpha-diastase can be used as the pollen abortion gene and can be originated from genes of rice endogenesis, millet, broomcorn, barley, wheat and other gramineous plants; the plant alpha-diastase is very good for transgenic plant of pollen abortion. The plant alpha-diastase gene has accurate expression level and can control the spreading of transgenosis; the plant alpha-diastase gene also can keep and breed a male sterility line while leave out the artificial castration step during the hybrid seed production process; the application prospect is wide.

The invention provides application of an MDPCR (multiple digital PCR (polymerase chain reaction)) technology to chromosome aneuploidy screening. Concretely, the invention discloses a method for performing NIPS (non-invas ive prenatal screening) including Down's syndrome, Edward's syndrome, Patau syndrome and other chromosome abnormality diseases on pregnant women by the MDPCR technology. The method has the advantages that early, safe, non-invas, accurate and fast effects are achieved, the method is suitable for large-scale detection and clinic application, and the like; intrauterine infection, abortion and death in the pregnancy middle and later periods of the pregnant women can be avoided; the goals of bearing and rearing better children are achieved.

The invention discloses a hippocampus kelloggi artificial breeding method. According to the method, male and female hippocampuses, which are aged 1-2 and are healthy, intact, large and fat, are selected to be parent hippocampuses and then separately raised inside nursery ponds; after a period of intensive breeding, the gonad of the parent hippocampuses are nearly mature, during the breeding process, artificial heating is necessary, then the parent hippocampuses are polycultured and then spawn at a individual ratio of 1:1; female hippocampuses in the parenting period are intensively bred and protected, the operation is required to be soft and rapid, the out-water duration cannot be too long and the temperature difference should be less than 2-3 DEG C for avoiding abortion of the male hippocampuses; after giving birth, the male hippocampuses need to be removed out immediately for being bred away from hippocampus fry and accordingly are prevented from devouring or harming the hippocampus fry. The hippocampus kelloggi artificial breeding method provides technical support and theoretical foundation for large-scale commercialized hippocampus culture. According to the hippocampus kelloggi artificial breeding method, the breeding cost is reduced by 50-60%, breeding water quality can be easily controlled through the detection of various water quality indicators before hippocampus stocking, further high survival rate and high growth rate of the hippocampuses can be guaranteed.

The present invention relates to Chinese medicine composition and its preparation, and is especially one kind of Chinese medicine composition for treating deficiency of both qi and blood, abnormal menstruation and fetal irritability and its preparation process. The Chinese medicine composition is preferably prepared into granule and capsule.

The invention discloses a culture method for triploid grape culture by embryo rescue and early identification of ploidy. The invention aims to cultivate triploid grape by embryo rescue and overcome the problem of seed abortion of hybrid embryo between diploid and tetraploid grape cultivars in conventional breeding. After a hybrid seed is obtained, chromosome count is performed by the combination of flow cytometry and wall degradation hypotonic method to identify the ploidy of the hybrid progeny. The invention increases the breeding efficiency of triploid grape, accelerates the breed selection of triploid grape variety and provides a new approach to the culture of seedless grape variety.

The invention relates to a non-polluted grape culture technique of seedless grape; the main steps are as follows: a first step: conventional management is carried out in prophase; a second step: from 15 days before blooming to 1 week before blooming, 15-20mg / L of GA3, 10-25mg / L of 4-CPA, 1g / L of KH2PO4 and an NO.1 synergist are adopted for dipping fruit cluster; a third step: 10-20 days after blooming, 15-20mg / L of GA3, 25mg / L of 4-CPA, 1g / L of KH2PO4 and an NO.2 synergist are adopted for dipping fruit cluster; other management is operated according to conventions. By carrying out seedless treatment on seedy grape, the method can delay the formation of grape megaspore, affect the normal fertilization process, and promote seed abortion to form parthenocarpic seedless fruit; meanwhile, the method can remarkablely reduce pesticide dose, and ultimately realize the purpose of pollution-free and seedless cultivation, thus having remarkable social and economic benefits.

The invention discloses special dietary seafood for postabortal and postpartum women. The special dietary seafood is prepared by mixing food-borne marine oligopeptide serving as a main raw material, active polysaccharide of a marine organism, dried marine algae powder, nutrient amino acid, oligosaccharide, nutrients, vitamins and mineral substances. The special dietary seafood can be prepared into powdery or granular food which is taken by dissolving in warm boiled water. The special dietary seafood is easy to dissolve, has the maximum biological absorbance and the maximum bioavailability to a human body, provides marine high-grade protein, active factors and nutrient components for the body, supplements various vitamins and mineral substances which are necessary to the human body, contributes to improving the nutritional status of the postabortal and postpartum women, can quickly restore the physique and improve the immunity of the body through the combined action of various factors such as the food-borne marine oligopeptide, bioactive ingredients in the active polysaccharide of the marine organism, the dried marine algae powder, the nutrient amino acid and the like, prevents changes in figure after abortion, keeps smooth skin, and is suitable for the postabortal and postpartum women.

The invention belongs to the field of gene engineering and relates to a serum lncRNA marker for diagnosis of unexplained recurrent spontaneous abortion, a primer group, application and a kit. The serum lncRNA marker comprises first lncRNA with homology being more than 90 percent with a nucleotide sequence as shown in SEQ ID NO:1, second lncRNA with homology being more than 90 percent with a nucleotide sequence as shown in SEQ ID NO:2, third lncRNA with homology being more than 90 percent with a nucleotide sequence as shown in SEQ ID NO:3, fourth lncRNA with homology being more than 90 percent with a nucleotide sequence as shown in SEQ ID NO:4, fifth lncRNA with homology being more than 90 percent with a nucleotide sequence as shown in SEQ ID NO:5 and sixth lncRNA with homology being more than 90 percent with a nucleotide sequence as shown in SEQ ID NO:6. The serum lncRNA marker is a novel biomarker and can greatly improve the sensitivity and the specificity of diagnosis of recurrent spontaneous abortion.

Disclosed is a Chinese compound formulation for nourishing blood, regulating menstruation, stopping bleeding to prevent abortion and preparation method, which is prepared from prepared rehmannia root, Ligusticum wallichii, root of herbaceous peony, Chinese mugwort leafmulberry leaf, Chinese angelica root, licorice root and donkey-hide gelatin, and can be made into preparations with the forms of electuary, granule, tablet or capsule.

The invention discloses a method for breeding a thermo-photoperiod sensitive dual-purpose genic male sterile Guangxuan S. The method for breeding the thermo-photoperiod sensitive dual-purpose genic male sterile Guangxuan S comprises the following steps: (1) taking Guangzhan 63S as a female parent and Guanghui7849 as a male parent; (2) hybridizing to obtain a hybrid F1, and mixing and harvesting a small amount of F1 seeds; (3) planting F2, selecting 12 low-panicle-enclosure and typical abortion and sterile plants to obtain F3 seeds, and planting F3 in 12 plant rows; (4) selecting three single plant line groups, cutting stubbles, and regenerating to obtain selfing seeds F4; (5) carrying out generation adding to obtain F5; (6) adopting shuttle breeding, namely south-reproduction and north-selection, from a generation F4, and synchronously breeding male sterile lines in a generation F8, wherein a male sterile line generation reaches up to F12 and is named as Guangxuan S. Rice quality of Guang18S obtained by adopting the method disclosed by the invention is as follows: coarse rice rate is 81.39%, milled rice rate is 74.4%, head milled rice rate is 71.3%, chalky kernel percentage is 21%, chalk degree is 1.5%, transparency is 1%, and grain length is 6.5mm.

The invention provides a noninvasive fetus 21, 18 and 13 trisomic syndrome antenatal detection positive quality control product and a preparing method thereof, and belongs to the fetus chromosome aneuploid noninvasive antenatal detection range. The preparing method of the positive quality control product includes the steps that DNAs of abortion groups of fetuses suffering from 21, 18 and 13 trisomic syndromes are broken into DNA fragments with the length ranging 160 bp to 200 bp; the abortion group DNA fragments recycled after breaking are added into mixed plasma in proportion; and the total plasma free DNAs are finally extracted, and the positive quality control products with different concentrations are prepared after attenuation with ultrapure water is carried out. The positive quality control product is a white transparent DNA solution, and has the beneficial effects of being stable, easy to store, convenient and rapid to use, non-toxic and the like; in addition, deviations of detection results of other samples in the same batch can be effectively avoided; and the accuracy and the reliability of the 21, 18 and 13 trisomic syndrome noninvasive antenatal detection are improved.

The present invention relates to a novel method of determining the existence of a normal pregnancy which has a high likelihood of culminating in a term pregnancy, by measuring hyperglycosylated hCG in a pregnant woman and comparing the concentration of measured hyperglycosylated hCG with a predetermined value. A measurement of hyperglycosylated hCG above the predetermined value, for example, about 13 ng / ml, is evidence of a high likelihood of a normal pregnancy. A measurement below the predetermined value is evidence of an abnormal pregnancy (ectopic pregnancy or miscarriage). Further analysis of the patient in the event that the measurement falls below the predetermined value is made by intravaginal or abdominal ultrasound to determine whether or not the pregnancy is ectopic or will likely result in a spontaneous abortion (miscarriage). This point-of-care (POC), over-the-counter (OTC) or professional laboratory application is unexpectedly rapid and highly predictive of pregnancy outcome in women exhibiting symptoms of pregnancy.

The invention discloses a method for identifying embryo abortion associated proteins of distant hybridization of chrysanthemum, and belongs to the field of chrysanthemum breeding and proteomics. The method comprises the steps of planting the small chrysanthemum as a female parent, taking wild chrysanthemum nankingense as a male parent for distant hybridization, cutting off pollinated inflorescences at different times after pollination, sampling ovaries with complete and plump morphological development and ovaries with abnormal and hollow morphological development respectively, immediately quick-freezing in liquid nitrogen after sampling, storing in a refrigerator at -80 DEG C, extracting proteins from samples by a TCA (Trichloroacetic Acid) / acetone precipitation method, quickly identifying differential proteins by an iTRAQ (Isobaric Tags for Relative and Absolute Quantitation) and mass-spectrometric technique, performing bioinformatic analysis, and obtaining the embryo abortion associated protein in the distant hybridization breeding of the chrysanthemum. The method can quickly and accurately identify the embryo differential proteins with different morphologies in different developmental stages during embryonic development of the chrysanthemum for the chrysanthemum and affinis wild species of the chrysanthemum, and a research foundation is provided for solving embryo abortion of the distant hybridization of the chrysanthemum.

The invention provides a vaginal tightening, cleaning and anti-inflammation traditional Chinese medicine composition for women. The composition is prepared from, by weight, 25-70 parts of common cruculigo rhizome extracts, 20-45 parts of radix angelica sinensis, 15-45 parts of fructus cnidii, 20-50 parts of pericarpium granati, 5-40 parts of saussurea involucrata, 3-15 parts of radix sophora flavescens, 5-15 parts of cortex phellodendri, 5-40 parts of fructus kochiae, 5-10 parts of radix stemonae, 5-55 parts of epimedium, 5-38 parts of cistanche deserticola, 5-10 parts of chlorhexidine acetate, 2-20 parts of ginsenosides, 10-25 parts of tenodera aridifolia stoll and 10-22 parts of ginkgo. The composition has the effects of repairing vaginal mucosae and healing cervical injuries and the functions of repairing elasticity and nourishing and regenerating the pelvic floor muscles and ligaments around the vagina, and can be used in private parts of women and easily absorbed by the skin and the mucosae. The composition can be used for recovering the vaginal tightness and elasticity, and vaginal relaxation, flatus vaginalis and urine leakage phenomena caused by various reasons such as parturition, abortion, frequent sexual activities, aging and premature ovarian failure are alleviated. The traditional Chinese medicine composition is mainly used for contracting and tightening the vaginae of the women and has the function of uterus warming, detoxification, sterilization, anti-inflammation, vagina nourishing and protecting and the like.

The present invention relates to a kit for detecting microorganisms, particularly a kit for detection of abortion chlamydia psittaci for pigs, sheep and cattle detection, and a preparation method and a use method thereof. The kit for detection of abortion chlamydia psittaci of the present invention comprises two pairs of specific primers composed of an outer primer and an inner primer, wherein the sequence of an upstream outer primer F3 is SEQ ID No. 1; the sequence of a downstream outer primer B3 is SEQ ID No. 2; the sequence of an upstream inner primer FIP is SEQ ID No. 3; and the sequence of an downstream inner primer BIP is SEQ ID No. 4. The kit of the invention has high sensitivity and high specificity, is simple and convenient in the detection method, and can be applicable to detection of pigs, sheep and cattle.

The invention discloses a medicine for treating undesirable involution of uterus after childbirth or abortion. The medicine is prepared by the following raw materials by weight: 9 parts of baked ginger, 12 parts of Chinese angelica, 9 parts of ligusticum wallichii, 12 parts of rhizoma corydalis, 6 parts of fructus evodiae, 15 parts of raw pollen typhae, 15 parts of fried lucid ganoderma, 15 parts of fried radix paeoniae alba, 12 parts of rhizoma cyperi, 9 parts of fried fennel, 10 parts of cinnamon, 20 parts of astragalus membranaceus, 15 parts of fried bighead atractylodes rhizome and 6 parts of liquorice. According to pilot study, the medicine for treating undesirable involution of uterus after childbirth or abortion positively promotes uterine contraction and reduces bleeding, causes no side effect or stimulation on uterine contraction, does not damage hepatorenal function and is easy to use, low in cost and capable of being accepted by patients.

The invention provides a restructing adenovirus of 2-type pig-ring virus and constructing method thereof, and the preventing appliances of pigs' relevant diseases caused by PCV2, including: augment and clone of PCV2 ORF2 genes; construction of recombinant shuttle vectors containing ORF2 genes; construction of recombinant adenovirus plasmid vectors; acquirement of recombinant adenovirus. Said recombinant adenovirus could determinately grow inside the bodies and continueously express ORF2 proteins to stimulate the organisms which generate neutralizing antibodies for ORF2 proteins, and offer endurance protection to pigs with promising future in the prevention and cure of PCV2, which could prevent efficiently poor quality of male-pig sperms, abortion, dead fetus, mummy and low production of female-pigs to increase the uniformity of pigs greatly.

The invention relates to the field of cross breeding of plants and particularly relates to an interspecific hybrid embryo rescuing and seedling forming method of sweet cherries in southern China and Chinese cherries. The method combines advantages of European sweet cherries and the Chinese cherries, and takes the 'European sweet cherries' as a female parent and the 'Chinese cherries' as a male parent to carry out interspecific distant hybridization. Aiming at the abortion problem of a distant hybrid embryo, the hybrid embryo is subjected to embryo rescuing. A proper sampling period of the embryo rescuing of each hybrid combination, concentrations and ratios of respective exogenous growth regulators for hybrid young embryo germination, subculture propagation and rooting culture, and a proper acclimatization and transplanting method are determined. By applying an interspecific hybrid embryo rescuing system of the sweet cherries in southern China and the Chinese cherries, which is established by the technology, the embryo germination rate reaches 80.00 percent, the multiplication coefficient of subculture hybridized new shoots reaches 5.0, the rooting rate of rooting culture is 90.91percent, the growth vigor of embryo culture seedlings is good and the seedlings grow robustly and have green leaves, and the acclimatization and transplanting survival rate reaches 77.27 percent.

The invention discloses a kit for multiplex real-time quantitative PCR (polymerase chain reaction) detection of chromosome aneuploid and application of the kit. The kit comprises n1 primer pairs used for amplifying multiple genes and non-genetic locus of a target chromosome and related probes and n2 primer pairs used for amplifying multiple genes and non-genetic locus of a reference chromosome and related probes, wherein the target chromosome is a chromosome subject to chromosome quantitative variation diseases, the reference chromosome is one or multiple chromosomes, and n1 and n2 are greater than or equal to 1 respectively. The kit can be used for noninvasive prenatal screening of Down's syndrome, Edward's syndrome, PaDow's syndrome and other chromosome abnormality diseases of pregnant women. The kit has the advantages of early stage, safety, noninvasiveness, accuracy, quickness and suitability for large-scale detection and clinical application, intrauterine infection, abortion and death of fetuses at middle and late stages of pregnancy of women can be avoided, and the objective of bearing and rearing better children is achieved.

The invention relates to a tissue culturing and rapid seedling raising method for Ludisia discolor (Ker-Gaw 1.)A.Rich seeds. The method comprises the following steps: 1. culture material selection; 2, induction culture of protocorm; 3, multiplication culture of the protocorm; 4, differentiation and strong seedling culture. According to the method, the problems of germination difficulty of Ludisia discolor (Ker-Gaw 1.)A.Rich in a natural state as well as inconsistent seed maturity degrees, short service lives of the seeds and large amount of abortion caused by short developmental cycle of fruit are solved, and one method capable of rapidly reproducing Ludisia discolor (Ker-Gaw 1.)A.Rich seedlings is developed. The method has the advantages of convenient-to-obtain materials and high success rate and can be used for saving Ludisia discolor (Ker-Gaw 1.)A.Rich fruit with inconsistent seed development, so that large quantity of the Ludisia discolor (Ker-Gaw 1.)A.Rich seedlings can be produced, and the Ludisia discolor (Ker-Gaw 1.)A.Rich resources are better protected.

The invention discloses an MYB (Myeloblastosis) gene PeMYB103 (Petal Epidermis Myeloblastosis 103) related to Euramerican populus (Populus X euramericana) anther development and an RNAi (Ribose Nucleic Acid interfere) vector thereof. In the invention, an anther specific MYB gene PeMYB103 is obtained from the Euramerican populus for the first time; and a nuclear localization transcription factor consisting of 311 amino acids is encoded by the gene. The homology between the PeMYB103 and an important transcripton AtMYB103 for controlling the development of an Arabidopsis anther is 52%. In the invention, an RNAi vector of the PeMYB103 is constructed and used for reducing or suppressing the expression of the PeMYB103 to obtain a transgenic plant with pollen abortion. The gene disclosed by the invention can be potentially used in creating a male sterile line in a genetic engineering way, in particular breeding a new variety of the populus.