24 results about "Abortion" patented technology

The invention belongs to the technical field of reproductive medicine and clinical metabolism intervention, and particularly relates to a group of markers for diagnosing unexplained recurrent abortion and application thereof. Aiming at the clinical pain point that unexplained recurrent abortion (URSA) lacks accurate metabolic typing diagnosis indexes and targeted treatment means, based on detection of a large number of clinical samples and verification of cell experiments, direct association between down-regulation of levels of choline related metabolites and trimethylamine oxide (TMAO) of decidua tissues and the RSA is disclosed for the first time; the core effect of TMAO in-situ synthesis of decidua stromal cells (DSC) in regulation and control of the decidualization process is determined; meanwhile, an RSA indication screening system of joint detection of serum creatinine and decidua choline metabolism is constructed, a targeted TMAO targeted intervention scheme is formed in a matched mode, a diagnosis and treatment closed loop of RSA from precise typing to targeted treatment is achieved, and a brand new technical path is provided for clinically solving the URSA diagnosis and treatment problem.

The invention relates to a preparation method of a placenta trophoblast-derived transporter and application of the placenta trophoblast-derived transporter in a medicine for treating spontaneous abortion, which comprises the following steps of: taking out a placenta of a suckling mouse, cutting the placenta into fine tissue blocks, resuspending by using digestive juice, digesting in a water bath kettle at 37 DEG C for 20 minutes, and then stopping digestion by using 1640 culture containing high-temperature heat-inactivated fetal calf serum; diluting and neutralizing collagenase digestive juice according to 5 times of volume; and centrifuging the collagenase digestive juice for multiple times to obtain a precipitate, namely the exosome. And carrying out density gradient centrifugation on the coarse migration body by using Optiprep as a density medium, washing, precipitating and centrifuging by using PBS on an ultra-high-speed centrifuge by using a horizontal rotor to obtain the placental trophoblast source migration body. The invention finds that the generation of the placental trophoblast cell source migration body is related to the physiological process of pregnancy for the first time, and the placental trophoblast cell source migration body can enter the circulatory system and reach the placenta through in-vitro supplementation.

The present application belongs to the field of biological medicine and molecular biology technology, and particularly relates to a marker for predicting the occurrence of repeated implantation failure and application thereof. The present application finds that abnormal lipid peroxidation in uterine epithelium can cause implantation defects, and the lipid peroxidation level of the uterus in patients with repeated implantation failure or repeated spontaneous abortion is enhanced, wherein the content of active aldehyde substance MDA and non-heme iron in serum can be used as a new non-invasive clinical index for predicting RIF patients; further, eliminating uterine lipid ROS to improve endometrial receptivity can be used as a potential new treatment strategy to improve embryo implantation and clinical pregnancy rate, and therefore has good practical application value.

The present application belongs to the technical field of genetic engineering, and relates to application of a tobacco chloroplast psbY gene or a coded protein thereof in regulation of abortion of tobacco. The present application causes shortened tobacco filaments and abnormal pollination by overexpression of the psbY gene, thereby causing abortion of the tobacco. Meanwhile, a breeding method for a tobacco male sterile line is proposed based on the application, that is, a transformation vector containing the tobacco chloroplast psbY gene is transformed into the genome of the tobacco by using an agrobacterium-mediated genetic transformation method, and a tobacco male sterile line variety with overexpression of the psbY gene is obtained. The present application firstly discovers and proves that the tobacco chloroplast protein psbY can be used for regulation of abortion of the tobacco, overexpression of the psbY gene can cause shortened tobacco filaments, the stigma is far higher than the anther, and the tobacco cannot be normally pollinated, thereby causing abortion of the tobacco. This has important theoretical significance and practical value for research on the tobacco male sterility, and creates a new prospect for tobacco breeding.

The invention relates to application of arachidonic acid (ARA) in preparation of a medicine for treating sleep disorder related spontaneous abortion. Peripheral blood lipid metabolism sequencing analysis finds that the ARA content in peripheral blood serum of a patient suffering from sleep disorder and repeated pregnancy loss is most obviously reduced compared with that of a patient suffering from normal sleep and normal pregnancy; an enzyme-linked immunosorbent assay test shows that the content of ARA in decidua stromal cells (DSCs) of a patient with sleep disorder and repeated pregnancy loss is remarkably reduced compared with that of a pregnant person with normal sleep; after the endometrial stromal cells (ESCs) are subjected to ARA treatment, the in-vitro decidualization level is increased; the ARA inhibitor can be used for inhibiting the in-vitro deciduation level of the ESCs; compared with a mouse with normal sleep, the content of ARA in peripheral blood and DSCs of a mouse with sleep disorder is remarkably reduced, decidua is poor, and the abortion rate is increased; supplementing ARA can improve decidua disorder and high abortion rate caused by sleep disorder. Therefore, a potential target and a new idea are provided for diagnosis and treatment of repeated pregnancy loss related to sleep disorder.

The invention relates to the field of animal husbandry molecular breeding and precise breeding, in particular to an animal husbandry improved variety breeding twin-fetal cattle system based on gene screening. The system comprises an ovulation potential typing module, a uterine bearing capacity quantification module, a sex compatibility verification module and a decision module. Constructing a combined evaluation model of the double-ovulation basis and the uterus metabolism capability by systematically integrating site specific amplification and gene expression quantification technologies; the core of the method is that a precise reproduction instruction is generated according to full-link data of genotypes and phenotypes; according to the method, a single ovulation rate screening mode is changed, the problem of abortion and weak abortion caused by insufficient intrauterine environment is effectively solved, and the success rate of double-fetal pregnancy is remarkably increased.

The application discloses application of CD52 protein in preparation of a medicine for treating recurrent spontaneous abortion, and belongs to the technical field of biological medicine. It is found that the CD52 protein can target and inhibit abnormal activation of effector T cells at a maternal-fetal interface, reduce release of proinflammatory factors (such as IFN-gamma), thereby reversing immune tolerance imbalance and improving pregnancy outcome. The CD52 protein is applied to the treatment of recurrent spontaneous abortion for the first time, and has the advantages of high targeting and high safety.

The invention discloses an intelligent attribution method for a continuous integration assembly line abortion problem, and the method comprises the steps: collecting a key abortion log, a code change context, an assembly line configuration context and a domain knowledge template through a multi-dimensional data mining pipeline in a structured manner; a two-stage retrieval mechanism is combined with a position-sensitive reverse ranking fusion algorithm to accurately match historical related cases from a hierarchical abortion case knowledge base, and a retrieval enhancement generation technology is utilized to drive a large language model to perform thinking chain reasoning. And dynamically optimizing the attribution result through a confidence evaluation strategy, and reversely updating the audited case features to the knowledge base to form a self-supervision expansion capability. According to the method, fine-grained accurate classification of six types of typical assembly line abortion problems can be realized, a user can be helped to quickly and accurately judge problem types and abortion reasons and provide effective detailed description support, the workload of abortion problem investigation is greatly reduced, and the robustness of a continuous integrated assembly line is effectively guaranteed.

The invention relates to a feed capable of preventing abortion of pregnant horses and a preparation method and application thereof, the feed is composed of corn straw, leguminous forage and non-leguminous forage, the feed contains 0-10% by mass of leguminous forage, and the total amount of the corn straw and the leguminous forage contained in the feed is less than or equal to 50%. The composition, proportion and nutritional ingredients of leguminous forage, non-leguminous forage and corn straw in the pregnant horse feed are scientifically adjusted, the content of conjugated estrogen in pregnant horse urine is remarkably increased, the content of unknown impurities B and C in the pregnant horse urine is remarkably reduced, and the separated and purified conjugated estrogen raw material medicine has the advantages of being high in extraction rate, good in stability and the like. The adverse reactions such as diarrhea, abdominal distension and incapability of lying on the ground of the pregnant horse and the abortion rate of the pregnant horse are obviously reduced.

The application belongs to the technical field of biomarkers, and particularly relates to a marker for unexplained recurrent spontaneous abortion and application thereof. The marker is at least one of LARP1, LSM1 and NCBP2. The application has the advantages that in URSA, the expression of 6 m7G regulatory factors is abnormal: LSM1 and NUDT16 are up-regulated, and IFIT5, LARP1, NCBP2 and NUDT4 are down-regulated (P<0.05). Machine learning determines that LSM1, LARP1 and NCBP2 are high-performance diagnostic biomarkers, and the diagnostic value thereof is verified by qRT-PCR and immunofluorescence in independent samples.

The invention provides application of a reagent for detecting KAT5 or H2A.Zac in preparation of a product for diagnosing placenta-derived diseases, and relates to the technical field of biomedicine. Experimental research of the inventor finds that chromosomes of trophoblasts in placentas of patients with repeated pregnancy loss are unstable, and expression of KAT5 and H2A.Zac is remarkably reduced. Experiments prove that KAT5-mediated H2A. Zac reduction is a main reason for reduction of trophoblast chromosome stability, so that the KAT5-H2A. Zac pathway provides a new action target for diagnosis and treatment of placenta-derived diseases, diagnosis and / or treatment of placenta-derived diseases can be realized, a new treatment strategy is provided for clinical improvement of poor pregnancy outcome, and the application of the KAT5-H2A. Zac pathway in diagnosis and treatment of placenta-derived diseases is developed. Therefore, the health level of female fertility can be improved, and gestation complications such as abortion and fetal growth limitation can be reduced.

The invention relates to the technical field of biology, in particular to application of human umbilical cord mesenchymal stem cells in preparation of drugs for recurrent spontaneous abortion and a pharmaceutical composition, and the human umbilical cord mesenchymal stem cells are used as NK cell migration and activation agents. According to the invention, the blank that the recurrent spontaneous abortion medicines do not have cellular medicines for regulating and controlling the migration and activation of the NK is filled up.

This invention, through lipidomics and clinical sample validation, found that prospective follow-up of pre-pregnancy serum samples from women with a history of recurrent pregnancy loss (RPL) revealed that the pre-pregnancy serum levels of phosphatidylinositol PI (18:1 / 18:1) (i.e., PI 36:2), phosphatidylcholine (29:0-6:0) (i.e., PC 35:0), phosphatidylethanolamine (18:1p-18:2) (i.e., PE 36:3p), triglycerides (28:1-10:4-11:2) (i.e., TG 49:7), triglycerides (14:0-18:2-20:5) (i.e., TG 52:7), and wax esters (22:1) (i.e., WE 22:1) were significantly lower in these women than in those who subsequently achieved successful pregnancies. This confirms that the pre-pregnancy serum levels of these six lipids (PI 36:2, PC 35:0, PE 36:3p, TG 49:7, TG 52:7, WE 22:1) were significantly lower in these women than in those who subsequently achieved successful pregnancies. Decreased levels of 22:1 lipids were significantly associated with the risk of miscarriage in subsequent pregnancies. The area under the curve (AUC) of the predictive model constructed by combining six lipids reached 0.85, demonstrating good predictive efficacy. Based on these findings, this lipid combination can serve as a reliable predictor of miscarriage risk in subsequent pregnancies in RPL patients and can be used to develop diagnostic reagents or kits for preconception risk screening.

The invention belongs to the technical field of biology, and discloses an application of Compound C in inhibition of uterine contraction of sheep, the Compound C significantly reduces expression of autophagy related genes LC3, BECN1, ATG5 and ATG7 by regulating an AMPK / mTOR signal channel, further inhibits autophagy of sheep uterine smooth muscle cells, and realizes targeted inhibition of uterine contraction. The optimal concentration of the Compound C in the preparation is 10 <-6 > mol / L, the administration dosage is 0.01 mg / kg of sheep body weight, the administration mode is intramuscular injection, and the Compound C can be combined with oxytocin to reverse the uterine contraction promoting effect of oxytocin. Cell experiments and animal experiments prove that the Compound C can improve uterine smooth muscle cell ultramicrostructure disorder, reduce autophagosome formation, delay the recovery speed of uterine horn of postpartum sheep, effectively inhibit abnormal uterine contraction and provide a new strategy for pregnancy maintenance and abortion reduction, and the action is milder.

The invention discloses an application method of a biomarker for auxiliary diagnosis of early spontaneous abortion. The application method comprises the following steps: collecting and preprocessing a sample; an HMGB1 concentration detection process; data analysis and quality control; performing diagnostic analysis and risk prediction; according to the clinical practical application and implementation, early-stage spontaneous abortion risk auxiliary diagnosis is achieved through standardized sample treatment, ELISA detection and multi-level data analysis, risk early warning can be provided before 8 weeks and even 5 weeks of pregnancy, hysteresis caused by current ultrasonic inspection is made up, and in the practical auxiliary diagnosis process, a single value can be seen, and time and labor are saved. Compared with the prior art, the method has the advantages that more attention is paid to the change trend, the prediction sensitivity and accuracy are greatly improved, the method is combined with existing clinical indexes including age and BMI to form a powerful prediction model, the accuracy is improved, a precious time window is provided for early-stage spontaneous abortion, effective risk early warning of early-stage spontaneous abortion is achieved, and the method is easy to apply and popularize clinically.

The invention relates to the technical field of paternity test, in particular to a method and equipment for identifying the parent-child relationship of abortion products and a storage medium, and the method comprises the following steps: firstly, obtaining abortion product samples, suspected parent genomic DNA and pregnant mother genomic DNA, and performing sequencing quality control comparison and typing to obtain respective genetic typing data; judging whether the abortion substance sample is a mixture or not, if the abortion substance sample is the mixture and the proportion of the maternal DNA exceeds a preset value, directly using three-party data to calculate and identify a parent-child relationship through a likelihood rate, and if the proportion does not reach the standard, reconstructing fetal genotyping by combining abortion substance and maternal data; if the sample is not a mixture, firstly confirming that the sample is a fetal tissue, and then obtaining a fetal genotype; and finally, comparing the fetus with the suspected parent gene typing, and obtaining a parent-child relationship identification result according to the mismatch rate. According to the technical scheme, accurate typing of the abortion product sample can be realized, interference of maternal cell residues on an identification result is avoided, and the accuracy of parent-child relationship identification of abortion products is improved.

This invention discloses sTREM1, a biomarker for assisting in the diagnosis of early spontaneous abortion, and its application, belonging to the field of biomedical technology. This invention is the first to discover a significant correlation between serum sTREM1 levels and early spontaneous abortion. In the early spontaneous abortion group and the 5-8 week gestation group, sTREM1 levels were significantly higher than those in the continued pregnancy group. Women experiencing early spontaneous abortion exhibit a unique dynamic pattern of progressively increasing sTREM1 levels, while those in the continued pregnancy show a slight decreasing trend. This invention further constructs a joint predictive model incorporating serum sTREM1 levels at 5-8 weeks of gestation, age ≥35 years, and polycystic ovary syndrome (PCOS), achieving an AUC as high as 0.823, demonstrating good discriminative and generalization abilities. Furthermore, decision curve analysis confirms that within the 10%-40% threshold probability range, the model's net benefit is superior to extreme treatment strategies. This invention is of great significance in the field of early spontaneous abortion risk prediction.

The application relates to a database management method and device, computer equipment, a storage medium and a computer program product. It relates to the field of digital twin technology and can be used in the field of financial technology or other related fields. The method comprises the following steps: in the case that a data access request is received, sending the data access request to a physical database and synchronously sending the data access request to a corresponding twin database of the physical database; in the twin database, performing an abortion operation on a processing request process corresponding to the data access request meeting an abortion execution strategy; and in the case that the running speed is greater than a first speed threshold, synchronously performing the abortion operation to the physical database to control the abortion of the processing request process corresponding to the data access request meeting the abortion execution strategy in the physical database. The method can avoid performance problems in the physical database as much as possible and improves the management effect of the physical database.

The invention relates to a drug abortion gestational sac collecting device which comprises a container, the upper end of the container is open, the container is used for collecting foreign matter discharged by the genital organ of a patient, a separating body is arranged in the container and used for separating the gestational sac and other liquid in the foreign matter, and a baffle is arranged on the side of the container and used for blocking excrements of the anus of the patient. According to the device, the whole process of gestational sac collection is optimized through cooperation of multiple components, and pollution prevention and control, separation efficiency and stability are all considered at the same time. The baffle can physically block excrement pollution, the pollution problem of synchronous diarrhea and pregnancy sac discharge is solved, and the cleanness of the specimen is guaranteed. The separating body efficiently separates the gestational sac from liquid, free-handed rummaging operation is omitted, and an accurate basis is provided for diagnosis and treatment. The support fixes the container and adapts to various toilet stools, loss or pollution caused by displacement is avoided, and the use reliability is improved.

The application discloses a biomarker for predicting unexplained recurrent spontaneous abortion and application thereof, and belongs to the technical field of molecular diagnosis and molecular biology. By comparing serum detection of 45 unexplained recurrent spontaneous abortion patients, it is found that the expression level of miR-142-3p in the serum of the unexplained recurrent spontaneous abortion patients is obviously higher than that of normal pregnant women. Compared with the normal pregnant women, the peripheral blood CD4 + CD25 + Treg cell level of the unexplained recurrent spontaneous abortion patients is significantly increased. By detecting the expression level of miR-142-3p, the unexplained recurrent spontaneous abortion patients can be pre-judged, so that the miR-142-3p can be used as an important index for clinically diagnosing and evaluating unexplained recurrent spontaneous abortion.

The invention discloses a drug abortion success rate prediction system and method based on a random forest, particularly relates to the technical field of medical data analysis, and aims to solve the problem that clinical doctors depend on experience to judge the success rate of drug abortion and lack scientific and quantitative prediction tools. The method comprises the following steps: firstly, selecting a target population which requires drug abortion and has no contraindication, and collecting clinical data of the target population; making a binarization coding rule, coding the collected clinical data of the target population item by item, and constructing a feature vector of the target population; based on the feature vector of the target population, predicting whether the drug abortion of the patient succeeds or not through a random forest model; then collecting the drug abortion outcome of the target population; and finally, merging the drug abortion outcome and the clinical data-data model of the patient to obtain a predicted value of the drug abortion outcome of the patient. According to the invention, the evaluation system for the adaptive population of the drug-induced abortion is established, the adaptive population of the drug-induced abortion is defined, and the failure rate of the drug-induced abortion is reduced.

The utility model discloses a simulation teaching aid for female rabbit pregnancy diagnosis practical training, and relates to the field of animal husbandry and veterinary teaching practical training equipment. A simulation teaching aid for female rabbit pregnancy diagnosis practical operation training comprises a diagnosis practical operation teaching aid and is characterized in that a placement cavity is formed in the diagnosis practical operation teaching aid; the inner core is internally provided with a uterus and a fetus and is placed in the placing cavity; according to the utility model, the risk of female rabbit abortion caused by incorrect manipulation or improper force application is avoided, the fetus touch skill can be repeatedly practiced in a safe environment, the operation proficiency is improved, from the perspective of teaching cost, the purchasing of breeding female rabbits is not needed, and complex and high-cost operations such as female rabbit aphrodisiac treatment and artificial insemination in the early stage are not needed, so that the working efficiency is improved. In addition, the simulation teaching aid is not limited by the mating period of the female rabbits, learners can carry out fetal-model practical training anytime and anywhere, and the limitation on time and space when living pregnant female rabbits are used for training is solved.

The invention belongs to the technical field of biological medicines, and particularly relates to application of fructose in prevention and treatment of recurrent abortion. According to systematic in-vivo and in-vitro studies, fructose promotes cholesterol accumulation and steroid hormone generation in trophoblasts, activates placenta syncytization signal channels and improves the expression level of HCG beta, so that placenta function maturation is promoted, the placenta secretion function is enhanced to maintain pregnancy, and the placenta secretion function is improved. The composition plays an important role in promoting placenta development, maintaining pregnancy and preventing and treating recurrent abortion. The discovery reveals the key function of fructose in regulating and controlling human trophoblastic function and maternal-fetal interface formation, and fructose can be further applied to preparation of drugs for promoting placenta development, maintaining pregnancy and preventing and treating recurrent abortion.

The present application relates to the technical field of molecular biology, and particularly relates to a primer group and a kit for detecting multiple pathogens through multiplex amplification targeted sequencing; the primer group comprises primers with nucleotide sequences shown in SQE ID NO. 1-SQE ID NO. 442, which are designed according to highly conserved and specific genomic regions of each pathogen, so as to ensure the specificity of amplification and the coverage of different variant strains; the primer group is suitable for prenatal TORCH screening, detection of pathogenic microorganisms of pregnant women with premature birth indications, screening of pathogenic microorganisms of suspected intrauterine infections, and detection of pathogenic microorganisms of unexplained abortion, and can simultaneously detect pathogenic microorganisms such as viruses, bacteria, fungi and parasites, and has high sensitivity and specificity.