202 results about "Heparin sodium" patented technology

Disclosed is a Taq DNA polymerase mutant. Compared to a wild-type Taq DNA polymerase, the Taq DNA polymerase mutant has a mutation at one or more of amino acid positions 116, 439, and 468, wherein the amino acid sequence of the wild-type Taq DNA polymerase is set forth in SEQ ID NO: 1. Compared to the wild-type Taq DNA polymerase, the Taq DNA polymerase mutant of the present invention has higher enzyme activity than the wild-type enzyme; both single-site mutants and multi-site mutants show improved tolerance to corn leaf supernatant, mouse serum, and / or heparin sodium, and thus can be used for direct rapid amplification of samples.

The invention relates to the technical field of odor treatment in heparin sodium production, and discloses an odor treatment device in heparin sodium production and a use method thereof.The odor treatment device comprises a case, the top of the case is in through connection with an air inlet pipe, the top of the case is in through connection with an exhaust pipe, and the inner wall of the air inlet pipe is fixedly connected with a filter layer and a power source connected with a motor; the rotating gears drive the corresponding bidirectional threaded rods to rotate synchronously, the turning threaded blocks drive the driving frames to operate synchronously through the mounting frames, the moving driving frames drive the sliding telescopic plates to stretch out and draw back, and in the process, the turning threaded blocks drive the sliding telescopic plates to stretch out and draw back in a sliding mode along the inner walls of the sliding grooves through the two mounting frames and the driving frames. At the moment, the waste gas entering the output square pipe flows out downwards through a gap between two sliding telescopic plates, the gas outlet position of the waste gas in the output square pipe is changed, and the situation that due to a gas outlet in a single position, partial microbial flora in a filtering layer develops in an unbalanced mode, and the treatment effect of equipment is affected is avoided.

The utility model discloses a stirring device for heparin sodium production, which comprises a fixing plate, the bottom surface of the fixing plate is fixedly connected with two right-angle plates, the upper surface of the fixing plate is fixedly connected with a stirring tank, the upper surface of the fixing plate is fixedly connected with two clamping plates, and the two clamping plates are fixedly connected with a connecting plate. The side faces, close to each other, of the two clamping plates are fixedly connected with the outer surface of the stirring tank, and an electric push rod is fixedly installed on the upper surface of each clamping plate. The sealing cover plate is supported by the electric push rod, so that a heparin sodium raw material can be conveniently injected into the stirring tank, the joint of the stirring tank and the sealing cover plate is sealed through the matched use of the driving motor, the sealing cover plate and the sealing ring, and the stirring tank is convenient to use through the matched use of the bearing, the spiral lifting blade and the mixing rod. The purpose of fully stirring the heparin sodium raw material is achieved, and the inner wall of the stirring tank can be conveniently cleaned through the cooperation of a cleaning plate and a scraping plate.

The invention provides hydrogel with a wound healing function and a preparation method thereof.The hydrogel is prepared from, by mass, 50-70 parts of basic gel matrix, 20-30 parts of natural medicine extract and 8-10 parts of bioactive ingredients; the natural medicine extract comprises superfine pearl powder, a semen cassiae extract, an aloe extract, a cortex phellodendri extract, a honeysuckle flower extract, a dandelion extract, a centipede extract, a fructus forsythiae extract and a green tea extract; the bioactive components comprise epidermal growth factors, heparin sodium, mussel mucin and nano-silver ions; on the basis of a commercially available compound cortex phellodendri liniment, further research and development are carried out, the superfine pearl powder, the semen cassiae extract, the aloe extract, the green tea extract and other natural medicine components are added, the optimal proportion is selected, and the basic gel matrix, the bioactive components and the like are combined, so that the wound healing rate, the antibacterial rate and the water holding rate of the hydrogel are remarkably improved finally.

The invention discloses blood-activating and stasis-removing massage cream and a preparation method thereof, and belongs to the technical field of daily chemical products. According to the massage cream capable of promoting blood circulation and removing stasis, active substances including caffeine, a ginger root extract, heparin sodium, oldenlandia diffusa, a pseudo-ginseng root extract, a carthamus tinctorius TINCTORIUS extract, a palmata pedunculata PALMATA extract and vanillyl butyl ether are added, and after the massage cream is smeared to a body for massage, the effects of moistening skin, removing stasis and promoting blood microcirculation can be achieved, so that the skin is healthier.

The invention discloses a Taq DNA polymerase mutant, compared with a wild type Taq DNA polymerase, the Taq DNA polymerase mutant has mutation at one or more of amino acid at the 116th site, amino acid at the 439th site and amino acid at the 468th site, and the amino acid sequence of the wild type Taq DNA polymerase is shown as SEQ ID NO: 1. Compared with a wild type Taq DNA polymerase, the Taq DNA polymerase mutant disclosed by the invention has the advantages that the enzyme activity is higher than that of the wild type Taq DNA polymerase, the tolerance of a single site mutant and a multi-site mutant to corn leaf supernatant, mouse serum and / or heparin sodium is improved, and the Taq DNA polymerase mutant can be used for direct and rapid amplification of samples.

The invention relates to the field of heparin sodium production, in particular to heparin sodium production odor treatment equipment which comprises a supporting bottom plate, and mounting frames are arranged at the two ends of the top of the supporting bottom plate; the device further comprises an aeration deodorization mechanism, the aeration deodorization mechanism comprises at least one aeration deodorization cylinder, and the aeration deodorization cylinder is installed on the installation frame; the aeration assembly is arranged at the bottom of the supporting bottom plate; the stirring rod is arranged in the aeration deodorization cylinder, and a plurality of first stirring blades are mounted on the stirring rod; the biological deodorization mechanism comprises a biological deodorization tank, sealing covers are arranged at the two ends of the biological deodorization tank, rotating shafts are arranged at the centers of the sealing covers, and a plurality of second stirring blades are arranged on the rotating shafts at equal intervals; the activated carbon adsorption mechanism is arranged at the top of the mounting rack, and the odor generated in heparin sodium production can be effectively, conveniently and orderly deodorized, so that production and processing treatment of heparin sodium can be facilitated.

The invention relates to the technical field of medical instruments, in particular to a variable-diameter blood seepage prevention artificial blood vessel based on a multi-layer composite structure, which comprises a composite artificial blood vessel main body, and a bioactive composite layer is arranged on the outer side of a gradient pore polyester woven layer. The main body of the composite artificial blood vessel is composed of more units such as the first artificial blood vessel part, the second artificial blood vessel part, the third artificial blood vessel part and the fourth artificial blood vessel part, and the inner diameter difference between every two adjacent units is 2.0 mm, so that the variable-diameter structure is more suitable for natural blood vessels of people clinically; in addition, the bioactive composite layer comprises type I collagen, heparin sodium and VEGF sustained release microspheres, the collagen / heparin composite layer forms a hydrated gel barrier when in contact with blood, the errhysis volume is reduced to 1 / 16 of that of a traditional product, endothelial cell migration is promoted by utilizing TiOnanotube array layer and VEGF sustained release synergy, and in addition, under the pulsating pressure of 120 mmHg, through 1 million times of cycle tests, the effective rate of the collagen / heparin composite layer is greatly increased. The structural integrity retention rate is larger than 99%, and the mechanical fatigue resistance is high.

The utility model relates to the technical field of crude heparin sodium preparation, and provides an adsorption tank for crude heparin sodium preparation, which comprises an adsorption cylinder, the top of the adsorption cylinder is fixedly connected with a fixing frame, and the top of the fixing frame is fixedly connected with a first motor. Through the arrangement of the first motor, the rotating shaft, the rotating plate, the connecting rods and the clamping plates, when the first motor starts to operate, the rotating shaft drives the rotating plate to rotate, the rotating plate extrudes and pushes the two connecting rods, the two connecting rods drive the two clamping plates to horizontally move oppositely under the limiting action of the sliding grooves, and the clamping plates are clamped. And the clamping plate is separated from the interior of the handle, so that the fixing effect on the connecting plate is relieved, an operator can take down the connecting plate and the stirring blade through the handle, the function of conveniently disassembling and assembling the stirring blade is achieved, the efficiency of disassembling and assembling the stirring blade by the operator is improved, and the labor intensity of the operator is reduced.

The invention discloses a heparin sodium purification method and application thereof, and the purification method comprises the following steps: S1, enzymolysis pretreatment: adding an animal mucous membrane liquid into a reaction kettle, adjusting the pH value, and carrying out enzymolysis at the temperature; s2, ultrasonic-assisted treatment: applying ultrasonic waves to the enzymatic hydrolysate; s3, adding a protective agent, namely adding a monosaccharide or disaccharide protective agent; s4, purification with ion exchange resin: dynamically adsorbing the heparin sodium by using strongly basic anion exchange resin, and eluting; s5, ethanol precipitation and ultrafiltration: ethanol is added, heparin sodium is precipitated, and then micromolecular impurities are removed through ultrafiltration; and S6, freeze-drying and forming: freeze-drying to obtain a finished product of heparin sodium. The invention relates to the technical field of biological medicine. According to the purification method and application of the heparin sodium, efficient separation and purification of the heparin sodium are achieved through the synergistic effect of multiple steps of enzymolysis, ultrasonic-assisted treatment, protective agent stabilization, ion exchange resin purification, ethanol precipitation and the like, the titer and purity of the product are remarkably improved, and the purification method is suitable for industrial production.

The application discloses a processing equipment for crude heparin sodium and belongs to the field of heparin sodium preparation, which comprises a shell, a liquid discharge port arranged at the lower end of the shell, a cover one fixed at the upper end of the shell, a liquid inlet arranged on the cover one, and an adsorption assembly arranged in the shell. The adsorption assembly comprises a plurality of supporting pieces fixed to the inner bottom wall of the shell, a filter cartridge clamped to the upper ends of the supporting pieces, a cavity and a hollow cavity arranged in the filter cartridge, and a cover two fixed to the upper end of the filter cartridge and sealing the cavity. The cavity for placing the resin is arranged in the filter cartridge, the resin is confined in the filter cartridge, direct contact with the high-speed rotating stirring paddle is avoided, and the resin is not damaged by the high-speed rotating stirring paddle. The paddle in the hollow cavity can actively and continuously suck the liquid from the bottom of the filter cartridge, and force the liquid to penetrate the resin bed in the radial direction. The radial flow has a large cross-sectional area, a short path, and a more uniform fluid distribution, and the utilization rate of the resin is higher.

The invention discloses a human blood coagulation factor VIII and a preparation method thereof, and the preparation method comprises the following steps: S1, carrying out refrigerated centrifugation on cryoprecipitate to obtain an extracting solution; s2, mixing the extracting solution and the heparin sodium solution to obtain a dissolving solution; s3, adjusting the pH value of the dissolving solution to 6.2-6.4 by using hydrochloric acid, and controlling the temperature to be 20-30 DEG C to obtain an acid precipitate; s4, mixing the acid precipitate with polyethylene glycol to obtain a polyethylene glycol precipitate; s5, adding the carboxylated ferroferric oxide nano-particles into the polyethylene glycol precipitate, incubating, and carrying out magnetic separation to obtain a first separation solution; s6, performing centrifugal separation on the first separation liquid to obtain a second separation liquid; s7, clarifying and filtering the second separation liquid, and adjusting the pH value to 6.4-7.4 to obtain a clarified liquid; and S8, adding a stabilizer and an inactivator into the clear liquid, and carrying out water bath heat preservation to obtain a human blood coagulation factor VIII stock solution. The method has the effect of improving the recovery activity and purity of the human blood coagulation factor VIII.

The application discloses heparin sodium cream and a preparation method thereof, and belongs to the technical field of pharmaceutical preparation production. The heparin sodium cream comprises the following components: heparin sodium, propylene glycol, hydroxyphenyl ethyl ester, sodium dodecyl sulfate, glycerol monostearate and distearate, octadecanol, liquid paraffin, white vaseline and water. The preparation method comprises the following steps: preparing an oil phase and mixing an aqueous phase; taking part of the mixed aqueous phase and the oil phase to form a water-in-oil type primary emulsion; then adding the remaining mixed aqueous phase into the primary emulsion, inducing phase transition into an oil-in-water type cream under mild conditions, and obtaining the finished product after vacuum defoaming and gradient cooling. The application realizes high-efficiency emulsification under mild conditions below 80 DEG C and 3000 rpm through a controlled phase inversion emulsification process, so that the particle size (D99) of the emulsion droplets is less than 5 microns, and the problem that the active substance heparin sodium loses activity under severe conditions is solved completely, and the stability of the product is improved significantly.

The invention discloses a production device for extracting sodium heparin by utilizing pig small intestinal mucosa, comprising a feeding roller, a sleeve rod and a base, wherein the sleeve rod comprises an upper sleeve rod and a lower sleeve rod connected to the lower part of the upper sleeve rod, a deep hole 1 cooperating with the upper sleeve rod is provided on the upper part of the base, a deep hole 2 cooperating with the lower sleeve rod is provided at the bottom of the deep hole 1, the deep hole 2 passes through the lower side surface of the base, the upper sleeve rod and the lower sleeve rod are respectively located in the deep hole 1 and the deep hole 2, and an annular scraper is arranged on the outer wall of the upper sleeve rod. The invention can scrape off the mucosa in a long section of the small intestine, and at the same time does not affect the subsequent production of the small intestine as casing during the processing.

The utility model relates to the technical field of heparin sodium, in particular to a heparin sodium production wastewater treatment device, and solves the problems that in the prior art, a filter screen cannot be cleaned, impurities on the filter screen block the filter screen, the impurities cannot be collected into filter cloth, and the filter cloth cannot be detached. The heparin sodium production wastewater treatment device comprises a placement plate, an outer cylinder arranged at the center of the top of the placement plate and a driving assembly arranged at the bottom of the placement plate, an inner cylinder is arranged in the outer cylinder, a stirring structure is arranged in the inner cylinder, one end of the stirring structure penetrates through the inner cylinder and the outer cylinder through a shaft sleeve, and an output shaft of the driving assembly is connected with the end of the stirring structure. Impurities can be conveniently blocked and collected through the multiple filter plates, then the filter plates are taken out from the interior of the through groove, the filter plates can be rapidly cleaned or replaced, and the filter screen is prevented from being blocked.

The invention relates to the technical field of crude heparin sodium processing, in particular to crude heparin sodium impurity removal and purification equipment which comprises a system frame and an impurity removal and purification system erected on the system frame. A primary screening compartment is arranged at the feeding end of the impurity removal system, and double-layer staggered rotary crushing rollers are arranged in the primary screening compartment; the turnover flow guide box is provided with a hydraulic drive spiral flow guide plate; the filtering device comprises upper-layer transverse stainless steel filtering wires and lower-layer longitudinal ceramic filtering wires; the crystallizer is provided with a gradient cooling condensation module. The purification system comprises a centrifugal separator, a preheating guide cylinder and a dryer, the centrifugal separator is in butt joint with the crystallizer through a purification guide-in box, the bottom of the centrifugal separator is connected with the preheating guide cylinder through a heparin sodium guide-out cylinder, a steam jacket and a spiral blade are arranged in the centrifugal separator, the outlet end of the centrifugal separator is connected with the dryer, and a vacuumizing assembly is integrated on the side edge of the dryer. Through integrated design of crushing, crystallization, preheating and intelligent control, efficient impurity removal and purification of the crude heparin sodium are realized, and the product purity and the production efficiency are improved.

The utility model relates to the technical field of wastewater treatment equipment, and discloses a waste liquid treatment device for heparin sodium production, which comprises a treatment pond, a purification box is slidably connected to the inner wall of the treatment pond, a filter plate is detachably connected to the inner wall of the purification box, an air cylinder is mounted outside the treatment pond, and the filter plate is detachably connected to the inner wall of the purification box. A linkage plate is rotationally connected to the inner wall of the purification box, a rotating block is rotationally connected to the inner wall of the linkage plate, the driving end of the air cylinder is fixedly connected to the bottom of the rotating block, a linkage rod is movably connected to the inner wall of the purification box, and the other end of the linkage rod is movably connected to the inner wall of the linkage plate. According to the waste liquid treatment device, the linkage rod ascends and descends to drive the purification box and the filter plate to ascend and descend, so that an operator can conveniently replace purification substances in the purification box, and the device allows purified water to be separated more easily after waste liquid treatment and ensures that the treated water is purer.

The invention provides a method for 3D differentiation of pluripotent stem cells into definitive endoderm cells by using a bioreactor, a differentiation medium and a differentiation factor A / B are also included, and the medium and the differentiation factor A / B comprise the following components: an MCDB131 medium, 2.44 mM glucose, 29.28 mM sodium bicarbonate, 2% of HSA, 1% of GlutaMAX, 250 [mu] M Vitamin C, 0.002% of ITS-X, 1% w / v of Polyethylene glycol (PEG), 1 mg / ml of Heparin Sodium salt, 100 ng / mL of Activin A and 3 [mu] M of CHIR99021. The method uses the bioreactor to differentiate the pluripotent stem cells into the stereotyped endoderm cells in a 3D suspension manner, is simple to operate and high in differentiation efficiency, provides technical support for scientific research and industrial large-scale production, and has a good application prospect.

The invention relates to the technical field of enzymatic hydrolysis, and particularly to an enzymatic hydrolysis preparation method for low-molecular-weight heparin sodium. The method comprises the following steps: S1.1, pre-treating the sodium heparin and combining it with a protective agent to obtain a heparin complex precipitate; S1.2, performing a first enzymatic hydrolysis on the heparin complex precipitate to obtain a supernatant A and a precipitate; S1.3, adjusting the pH of the precipitate to release highly sulfated fragments to obtain a supernatant B; S1.4, performing a second enzymatic hydrolysis on the supernatant B to obtain a secondary enzymatic hydrolyzate, combining the supernatant A and the secondary enzymatic hydrolyzate, and filtering to obtain a retentate; S1.5, post-treating the retentate to obtain the low-molecular-weight heparin sodium; and using a composite protective agent prepared by covalently coupling citric acid-modified superparamagnetic Fe3O4 nanoparticles with aminocalixarene, accurately regulating the enzymatic hydrolysis path and achieving efficient separation, thereby solving the problems of uneven molecular weight, low yield, and complex purification in traditional processes.

The invention relates to the technical field of heparin sodium, and provides anti-FXa low-molecular heparin sodium as well as a preparation method and application thereof. The anti-FXa low-molecular heparin sodium is extracted from an enoxaparin sodium by-product generated in the process of preparing an enoxaparin sodium crude product from heparin sodium, the weight-average molecular weight of the anti-FXa low-molecular heparin sodium is 1000-2000 Da, and the anti-FXa activity of the anti-FXa low-molecular heparin sodium is 20-30 IU / mg. According to the technical scheme, the problem that the anti-FXa effect of the heparin sodium is poor due to the fact that the molecular weight of the heparin sodium is large in the prior art is solved.

The invention belongs to the technical field of polypeptide preparation and biological pharmacy, and particularly relates to eggshell membrane polypeptide with thrombin inhibition activity and application of the eggshell membrane polypeptide. According to the invention, two polypeptides are separated and purified from an eggshell membrane, and the two polypeptides are a nonapeptide YDGQHGRVS and an octapeptide EVPPSAL respectively. Tests prove that the nonapeptide YDGQHGRVS and the octapeptide EVPPSAL have thrombin inhibition activity equivalent to that of positive control heparin sodium, and discover that the nonapeptide YDGQHGRVS prolongs the activated partial thromboplastin time and the prothrombin time. The nonapeptide YDGQHGRVS and the octapeptide EVPPSAL provided by the invention are derived from eggshell membranes, are natural in source, good in thrombin inhibition activity and high in safety, and have a good application prospect.

The utility model discloses crushing equipment for heparin sodium production, and belongs to the technical field of crushing and cutting devices. Crushing equipment for heparin sodium production comprises an equipment shell and further comprises a first motor fixedly connected to the equipment shell, a second motor fixedly connected to the equipment shell and a third motor fixedly connected to the equipment shell. The rotating shaft is rotationally connected into the equipment shell, and the rotating shaft is fixedly connected with the output end of a first motor; the multiple sets of cutting knives are annularly and fixedly connected to the rotating shaft, and the cutting knives are located below the feeding end of the equipment shell; the telescopic cylinders are fixedly connected into the equipment shell; the pressing plate is connected into the equipment shell in a sliding mode, and the outer diameter of the pressing plate is matched with the inner diameter of the equipment shell; through the arrangement of the pressing plate and the cutting knife, frozen animal tissue can be extruded and fixed to a certain degree, the cutting knife can cut the animal tissue conveniently, the slipping and jumping conditions are avoided, and the frozen animal tissue can be smashed conveniently.

The utility model relates to the technical field of sausage scraping machines, in particular to a sausage scraping machine for heparin sodium and sausage casing production, which is provided with a sausage scraping mechanism capable of performing multi-model replacement and height adjustment, is simple to operate and high in sausage scraping efficiency, and improves the practicability. Comprising a platform plate, a top frame, a first sliding sleeve, a first sliding rod, a mounting sleeve frame, a mounting block, a bolt, a nut, a casing scraper, a jacking screw sleeve, a jacking screw rod and a jacking screwing handle, the outer side of the top end of the platform plate is connected with the bottom end of the top frame, the inner side of the middle of the top end of the top frame is connected with the outer side of the first sliding sleeve, and the inner wall of the first sliding sleeve is slidably connected with the outer wall of the first sliding rod; the bottom end of the first sliding rod is connected with the middle of the top end of the mounting sleeve frame, the middle of the front side of the first sliding sleeve is communicated with the inner side of a jacking thread sleeve, the inner wall of the jacking thread sleeve is in threaded connection with the outer wall of a jacking screw, the inner side of the mounting sleeve frame and the outer side of the mounting block are detachably inserted, and a bolt synchronously penetrates through the mounting block and the mounting sleeve frame to be in threaded connection through a nut on the extending side. The rear side of the platform plate is connected with a traction mechanism.

This utility model discloses a high-efficiency pretreatment device for heparin sodium raw materials, relating to the technical field of meat product processing equipment. It includes a processing box with an opening and closing door installed on its outer wall. An installation horizontal plate is fixed inside the processing box, and a drive plate is positioned above the installation horizontal plate. Multiple mucosal scrapers are fixed to the bottom of the drive plate. Multiple through holes are drilled through the installation horizontal plate for the mucosal scrapers to pass through. Clamping clamps are installed on the bottom of the installation horizontal plate to the side of the through holes, and are used to clamp the small intestine. Multiple water supply pipes are installed on the inner wall of the processing box, located below the installation horizontal plate. A water outlet pipe is connected to the bottom of the processing box. A support frame is fixed to the inner wall of the processing box, located below the water supply pipes. A filter plate is slidably connected to the top of the support frame. The device integrates washing, separation, and secondary cleaning within the same processing box, allowing for direct mucosal separation and cleaning of the mucosa after small intestine cleaning.

This invention discloses a device for draining heparin sodium precipitation solution, comprising a precipitation tank with an observation port on its side wall, a sealed top with a lid, a rotatably connected fixing seat on the lid, a support tube coaxially threadedly connected to the fixing seat, a second motor fastened to the top of the lid, gears connected to the output end of the second motor and the outer wall of the fixing seat, the gears meshing together; a connecting seat fastened to the bottom of the support tube inside the precipitation tank, a protective mesh cover installed at the bottom of the connecting seat, the end of the support tube extending into the protective mesh cover, and a guide rod penetrating the lid vertically connected to the top of the connecting seat; and a collection tank, the top of the support tube connected to a pump body via a hose, a transport pipe connected to the outlet of the pump body. This invention, through the separation and filtration functions of the protective mesh cover and filter screen, prevents the bottom sediment from being drawn into the collection tank, ensuring the purity of the extracted upper solution.

The invention relates to the field of stirring and mixing, in particular to a stirring device for a heparin sodium purification process, which comprises a stirring cylinder, a driving mechanism is connected to the stirring cylinder, a shaft tube is connected to the driving mechanism, two stirring parts are fixedly connected to the shaft tube, the two stirring parts are symmetrically arranged, each stirring part is of an internal hollow structure, and an opening is formed in one end of each stirring part. The upper end of the stirring part is rotationally connected with a cover plate, a torsional spring is installed at the rotating connection position of the cover plate and the stirring part, the upper end of the cover plate is fixedly connected with a conversion plate, the conversion plate is in an arc shape, a plurality of through grooves are formed in the cover plate, and a hook is fixedly connected to the position close to each through groove. The heparin sodium purification efficiency is further improved.

The application belongs to the technical field of crushing, and particularly relates to an intelligent crushing equipment for heparin sodium production, which comprises a supporting structure, a crusher is arranged at one side of the upper end of the supporting structure, a protection mechanism is arranged at the upper end of the crusher, a guide mechanism is arranged at the lower end outlet of the crusher, a conveying mechanism is arranged at one end of the supporting structure, and a grinding mechanism is arranged at the upper end of the conveying mechanism. The equipment is provided with the grinding mechanism, the grinding static disc is connected with the sleeve on the fixing ring through the air cylinder, the air cylinder piston rod can drive the grinding static disc to slide up and down, the gap between the grinding static disc and the grinding dynamic disc is adjusted in real time, the design can accurately control the grinding granularity, the needs of different fine processing stages of heparin sodium raw materials can be met, and excessive grinding or insufficient grinding is avoided; the air cylinder can adjust the pressure of the grinding static disc on the raw materials, cooperates with the rotation of the grinding dynamic disc, realizes the double grinding effect of extrusion and shearing on the raw materials, and improves the grinding efficiency and uniformity.

The invention relates to a nitric oxide donor based on heparin sodium and a preparation method and application thereof, the method comprises the following steps: (1) dissolving heparin sodium in a 2-(N-morpholino) ethanesulfonic acid buffer solution, adding an activator, and carrying out a reaction to obtain a reaction solution A; (2) adding beta-mercaptoethylamine into the reaction solution A, and continuously reacting to obtain a reaction solution B; (3) dialyzing the reaction solution B to obtain a solution C; and (4) adding tert-butyl nitrite into the solution C, and reacting to obtain the heparin sodium-based nitric oxide donor. Wherein in the step (1), the activating agent is 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide or hydrochloride of the 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide. According to the prepared heparin sodium-based nitric oxide donor, the nitric oxide release time is prolonged, and the problem that the half-life period of nitric oxide is short is solved.

The invention discloses a combined method for converting nadroparin calcium into calcium and adjusting molecular weight. The method comprises the following steps: degrading common heparin sodium by using a sodium nitrite solution, then reducing by using sodium borohydride, and finally carrying out alcohol precipitation, adding the obtained precipitate into purified water, stirring and dissolving, and carrying out ultraviolet radiation; adding into an ion exchange resin column, washing by using a 100-500mmol / L calcium chloride solution, eluting by using a 1-5mol / L calcium chloride solution, detecting the molecular weight and distribution, and collecting the eluent; adding hydrogen peroxide into the obtained feed liquid for oxidation, and then carrying out alcohol precipitation; and adding purified water into the obtained precipitate, dissolving, and freeze-drying. Calcium conversion and molecular weight adjustment can be achieved at the same time through only one procedure, the calcium content of the obtained nadroparin calcium meets the standard, and the molecular weight is basically consistent with the reference. The method is simple in process operation, greatly shortens the working procedure time, reduces the solvent, labor and power cost of multi-step reaction, and has remarkable economic benefits.

The invention provides a method for producing heparin sodium through microbial fermentation, relates to the technical field of polysaccharide preparation, and belongs to the IPC classification number C08B37. The method for producing the heparin sodium through microbial fermentation comprises the following steps: mashing porcine small intestine mucosa, performing high-pressure and ultrasonic treatment, inoculating compound microorganisms, performing anaerobic fermentation, performing resin adsorption, elution and alcohol precipitation on fermentation liquor, and collecting precipitates to obtain the heparin sodium. According to the invention, pig small intestine mucosa is mashed, and is subjected to high-pressure and ultrasonic treatment, so that dissolution of heparin sodium and nutritional ingredients is promoted, and the binding force of heparin sodium and other impurities is weakened; the compound microorganisms not only can decompose polysaccharide-protein compounds and intercellular substance components in mucous membranes, but also can degrade protein, fat and other impurity components in the mucous membranes, so that the interference of impurities on the extraction process of the heparin sodium is reduced; the resin can enrich the heparin sodium, so that the heparin sodium is separated from the fermentation liquor. All the steps have a synergistic effect, and the titer and the yield of the heparin sodium are improved.