285 results about "Heparin sodium" patented technology

The invention relates to the field of molecular diagnosis, in particular to an RPA-Cas12a one-step detection system and application thereof. The invention finds that heparin sodium has cleavage activity for inhibiting LbCas12a, and develops a one-step nucleic acid detection method compatible with RPA amplification and Cas12 detection based on the inhibition effect. The detection limit of the method reaches 0.6 (copy / microliter), and the method can be expanded to other two Cas12 subtype proteins, namely AsCas12a and AapCas12b. In the detection of monkey pox virus, influenza A virus and new coronavirus as examples, compared with quantitative PCR, the detection sensitivity and specificity of the method both reach 95% or above. The CRISPR one-step nucleic acid detection method has the advantages of high sensitivity and specificity, short detection time and the like, and the potential nucleic acid aerosol pollution risk of a traditional two-step nucleic acid detection method is removed.

Disclosed is a Taq DNA polymerase mutant. Compared to a wild-type Taq DNA polymerase, the Taq DNA polymerase mutant has a mutation at one or more of amino acid positions 116, 439, and 468, wherein the amino acid sequence of the wild-type Taq DNA polymerase is set forth in SEQ ID NO: 1. Compared to the wild-type Taq DNA polymerase, the Taq DNA polymerase mutant of the present invention has higher enzyme activity than the wild-type enzyme; both single-site mutants and multi-site mutants show improved tolerance to corn leaf supernatant, mouse serum, and / or heparin sodium, and thus can be used for direct rapid amplification of samples.

The invention provides a process for preparing a high-purity human blood coagulation factor IX from plasma. The process comprises the following steps: acquiring plasma; adding anion gel, adsorbing, and collecting an eluted protein solution; adding heparin sodium, filtering, ultra-filtering and dialyzing to obtain dialyzed protein liquid; adding an S / D solution; adding anion gel for secondary adsorption, and collecting eluted protein liquid II; performing filtration, ultrafiltration and dialysis, and collecting eluted protein liquid after dialysis; filtering is performed; carrying out affinity gel chromatography purification, collecting eluent, and carrying out ultrafiltration and dialysis; filtering, and adding a dialysate containing glycine for dialysis to obtain a human blood coagulation factor IX stock solution; adjusting the titer, and adding heparin sodium to obtain a diluted solution; pre-filtering the diluted liquid to obtain a semi-finished product of the human blood coagulation factor IX; sterilizing, filtering, sub-packaging, carrying out vacuum freeze drying, carrying out dry heat virus inactivation, inspecting and packaging. According to the invention, the production process is simplified, the cost is reduced, the activity of the finally obtained human blood coagulation factor IX is greater than 50IU / ml, and the specific activity is higher than 180IU / mg.

The invention relates to the technical field of odor treatment in heparin sodium production, and discloses an odor treatment device in heparin sodium production and a use method thereof.The odor treatment device comprises a case, the top of the case is in through connection with an air inlet pipe, the top of the case is in through connection with an exhaust pipe, and the inner wall of the air inlet pipe is fixedly connected with a filter layer and a power source connected with a motor; the rotating gears drive the corresponding bidirectional threaded rods to rotate synchronously, the turning threaded blocks drive the driving frames to operate synchronously through the mounting frames, the moving driving frames drive the sliding telescopic plates to stretch out and draw back, and in the process, the turning threaded blocks drive the sliding telescopic plates to stretch out and draw back in a sliding mode along the inner walls of the sliding grooves through the two mounting frames and the driving frames. At the moment, the waste gas entering the output square pipe flows out downwards through a gap between two sliding telescopic plates, the gas outlet position of the waste gas in the output square pipe is changed, and the situation that due to a gas outlet in a single position, partial microbial flora in a filtering layer develops in an unbalanced mode, and the treatment effect of equipment is affected is avoided.

The utility model discloses a heparin sodium processing device capable of quickly removing impurities, which belongs to the technical field of heparin sodium processing, and adopts the technical scheme that the heparin sodium processing device comprises an impurity removal barrel and a feed port, an impurity removal and discharge mechanism is arranged in the middle of the impurity removal barrel, and a high-temperature impurity removal mechanism is arranged on the side wall of the impurity removal barrel. The impurity removing and discharging mechanism comprises a discharging pipe, a storage tank, a rotating shaft, a motor, a roller, a stirring blade, a filter screen, an impurity discharging cover, a discharging opening, an electric telescopic rod and a blocking plate, and by arranging the impurity removing barrel, the feeding opening, the impurity removing and discharging mechanism and the high-temperature impurity removing mechanism, during use, a heparin sodium raw material enters the impurity removing barrel through the feeding opening; the speed of passing the heparin sodium through the filter screen can be increased through the impurity removing and filtering mechanism, the heparin sodium impurity removing and filtering efficiency is improved, meanwhile, filtered impurities can be rapidly discharged and cleaned, the heparin sodium in the impurity removing barrel can be heated through the high-temperature impurity removing mechanism, and moisture and bacteria in the heparin sodium can be subjected to impurity removing treatment.

The utility model provides a desorption device for extracting heparin sodium, and relates to the technical field of heparin sodium processing equipment, the desorption device comprises a main desorption tank body, a middle mixing rotating shaft and an upper annular pipe, the middle mixing rotating shaft is arranged in the main desorption tank body, the upper annular pipe is fixed on the upper part of the inner side of the main desorption tank body, two sides of the middle mixing rotating shaft are fixedly connected with outer cross frames, and the outer cross frames are fixedly connected with the middle mixing rotating shaft. Outer scraping strips are slidably connected to the outer sides of the outer transverse frames, inner supporting elastic pieces are arranged between the outer transverse frames and the outer scraping strips, inner transverse frames are symmetrically arranged on the two sides of the middle mixing rotating shaft, and a middle connecting shaft is slidably connected to the center of the middle mixing rotating shaft. The inner wall of the main desorption tank body is flushed through the outer cleaning nozzles, residues on the inner wall of the main desorption tank body are cleaned through the outer scraping strips, the cleaning efficiency of the inner wall of the main desorption tank body is improved through double treatment, the cleaning time is shortened, and the problems that a large amount of water needs to be accumulated for cleaning the tank body at a high position, and consequently the cleaning water consumption is large and time is consumed are solved.

The utility model discloses a stirring device for heparin sodium production, which comprises a fixing plate, the bottom surface of the fixing plate is fixedly connected with two right-angle plates, the upper surface of the fixing plate is fixedly connected with a stirring tank, the upper surface of the fixing plate is fixedly connected with two clamping plates, and the two clamping plates are fixedly connected with a connecting plate. The side faces, close to each other, of the two clamping plates are fixedly connected with the outer surface of the stirring tank, and an electric push rod is fixedly installed on the upper surface of each clamping plate. The sealing cover plate is supported by the electric push rod, so that a heparin sodium raw material can be conveniently injected into the stirring tank, the joint of the stirring tank and the sealing cover plate is sealed through the matched use of the driving motor, the sealing cover plate and the sealing ring, and the stirring tank is convenient to use through the matched use of the bearing, the spiral lifting blade and the mixing rod. The purpose of fully stirring the heparin sodium raw material is achieved, and the inner wall of the stirring tank can be conveniently cleaned through the cooperation of a cleaning plate and a scraping plate.

The invention provides hydrogel with a wound healing function and a preparation method thereof.The hydrogel is prepared from, by mass, 50-70 parts of basic gel matrix, 20-30 parts of natural medicine extract and 8-10 parts of bioactive ingredients; the natural medicine extract comprises superfine pearl powder, a semen cassiae extract, an aloe extract, a cortex phellodendri extract, a honeysuckle flower extract, a dandelion extract, a centipede extract, a fructus forsythiae extract and a green tea extract; the bioactive components comprise epidermal growth factors, heparin sodium, mussel mucin and nano-silver ions; on the basis of a commercially available compound cortex phellodendri liniment, further research and development are carried out, the superfine pearl powder, the semen cassiae extract, the aloe extract, the green tea extract and other natural medicine components are added, the optimal proportion is selected, and the basic gel matrix, the bioactive components and the like are combined, so that the wound healing rate, the antibacterial rate and the water holding rate of the hydrogel are remarkably improved finally.

The invention relates to an eye repair composition and application thereof. The eye repair composition comprises ecdysterol, a Tibetan kefir fermentation product and heparin sodium. The invention creatively finds that the composition composed of the ecdysterol, the Tibetan kefir fermentation product and the heparin sodium has remarkable effects on the aspects of improving the skin brightness, reducing the content of melanin around eyes and eliminating dark circles on the skin, especially the eye skin, and the ecdysterol, the Tibetan kefir fermentation product and the heparin sodium have a certain synergistic effect on the effects; the eye cream is very suitable for eye care products, mild, non-irritant and good in stability.

The utility model discloses a heparin sodium colored impurity removing device, which belongs to the technical field of heparin sodium, and adopts the technical scheme that the heparin sodium colored impurity removing device comprises a tank body, a mixing mechanism is arranged in the tank body, a separating assembly is arranged on one side of the mixing mechanism, and a reinforcing assembly is arranged in the tank body. The heparin sodium and the buffer solution can be fully mixed and subjected to multi-layer filtration through the mixing mechanism during use, the separation assembly is arranged and can be matched with the mixing mechanism to separate the mixing mechanism during use, the impurity filtering effect is improved, and the reinforcing assembly is arranged, so that the heparin sodium and the buffer solution are fully mixed and subjected to multi-layer filtration. The heparin sodium impurity filtering and removing effect can be improved through the reinforcing assembly in the using process, the feeding port can be expanded through the expanding assembly in the using process, the tank body can be moved more conveniently through the moving assembly in the using process, the tank body can be moved through the fixing assembly in the using process, and the heparin sodium impurity filtering and removing effect can be improved. And the operation of the tank body is more stable.

The utility model discloses a multistage precipitation and filtration device for heparin sodium, which belongs to the technical field of heparin sodium precipitation and filtration, and adopts the technical scheme that the multistage precipitation and filtration device comprises a tank body, a precipitation and filtration mechanism for precipitating the heparin sodium is arranged in the tank body, and a collection component for collecting the filtered heparin sodium is arranged outside the tank body. During use, firstly, the sealing cover is opened, a heparin sodium liquid is placed in the tank body, then the heparin sodium liquid falls into the precipitation frame, then a precipitant is poured into the precipitation frame and reacts with the heparin sodium liquid, then the sealing cover is closed and waits for a period of time, after precipitation is completed, the lead screw is rotated, and the heparin sodium liquid is separated from the precipitation frame. The precipitation frame descends along the lead screw, then the pure heparin sodium liquid is discharged out of the tank body through the filter cartridge and the discharging groove, and precipitates are accumulated in the precipitation frame.

The invention discloses blood-activating and stasis-removing massage cream and a preparation method thereof, and belongs to the technical field of daily chemical products. According to the massage cream capable of promoting blood circulation and removing stasis, active substances including caffeine, a ginger root extract, heparin sodium, oldenlandia diffusa, a pseudo-ginseng root extract, a carthamus tinctorius TINCTORIUS extract, a palmata pedunculata PALMATA extract and vanillyl butyl ether are added, and after the massage cream is smeared to a body for massage, the effects of moistening skin, removing stasis and promoting blood microcirculation can be achieved, so that the skin is healthier.

The invention discloses a Taq DNA polymerase mutant, compared with a wild type Taq DNA polymerase, the Taq DNA polymerase mutant has mutation at one or more of amino acid at the 116th site, amino acid at the 439th site and amino acid at the 468th site, and the amino acid sequence of the wild type Taq DNA polymerase is shown as SEQ ID NO: 1. Compared with a wild type Taq DNA polymerase, the Taq DNA polymerase mutant disclosed by the invention has the advantages that the enzyme activity is higher than that of the wild type Taq DNA polymerase, the tolerance of a single site mutant and a multi-site mutant to corn leaf supernatant, mouse serum and / or heparin sodium is improved, and the Taq DNA polymerase mutant can be used for direct and rapid amplification of samples.

The utility model discloses a heparin sodium extraction inactivation device, relates to the heparin sodium extraction technical field, including inactivation mechanism, tube cover mechanism, cleaning mechanism and other parts, the tube cover mechanism includes input tube, the upper port of input tube is provided with cover groove, the lateral side of cover groove is provided with four card slot in rectangular shape on the cover groove side, and the card slot is connected with the card slot. When an upper cover of the pipe cover mechanism of the device is disassembled, four clamping pieces can be moved out of clamping grooves only by rotating a knob through an assembly, then fixation of the upper cover can be relieved, and therefore disassembly of the upper cover becomes simple, workers can assemble and disassemble the upper cover conveniently, and then heparin sodium raw materials can be put in conveniently; after raw materials are discharged after inactivation, water in the water tank is pumped out through the water pump and fed into the annular pipe, all the nozzles are sprayed to the inner wall of the inactivation tank, and the water continuously washes the inner wall of the inactivation tank, so that the inner wall of the inactivation tank is cleaned, manual cleaning by workers is not needed, meanwhile, the water consumption for cleaning is low, and water is saved.

The invention relates to the field of heparin sodium production, in particular to heparin sodium production odor treatment equipment which comprises a supporting bottom plate, and mounting frames are arranged at the two ends of the top of the supporting bottom plate; the device further comprises an aeration deodorization mechanism, the aeration deodorization mechanism comprises at least one aeration deodorization cylinder, and the aeration deodorization cylinder is installed on the installation frame; the aeration assembly is arranged at the bottom of the supporting bottom plate; the stirring rod is arranged in the aeration deodorization cylinder, and a plurality of first stirring blades are mounted on the stirring rod; the biological deodorization mechanism comprises a biological deodorization tank, sealing covers are arranged at the two ends of the biological deodorization tank, rotating shafts are arranged at the centers of the sealing covers, and a plurality of second stirring blades are arranged on the rotating shafts at equal intervals; the activated carbon adsorption mechanism is arranged at the top of the mounting rack, and the odor generated in heparin sodium production can be effectively, conveniently and orderly deodorized, so that production and processing treatment of heparin sodium can be facilitated.

The invention discloses a PICC (Peripherally Inserted Central Catheter) system for preventing fibrin sheath, which comprises a PICC tube body and a resistance measuring device, the PICC tube body is provided with a resistance detection head which is used for measuring the resistance of blood when the PICC tube body is inserted into a superior vena cava, and the resistance detection head is connected with the resistance measuring device. When the resistance measuring equipment detects that the resistance of the blood detected by the resistance detecting head exceeds an initially set alarm value, it is indicated that a fibrin sheath is formed outside the PICC tube body, the resistance measuring equipment sends out alarm information, and medical staff immediately injects heparin sodium into the PICC tube body to dissolve the fibrin sheath after receiving the alarm information; the characteristic that blood and tissue resistance values are different is utilized, when the resistance values change, the buzzer gives an alarm to remind medical staff that fibrin sheaths are formed in the medical staff, the medical staff intervenes and eliminates the fibrin sheaths as soon as possible, and pain of a patient during tube drawing is relieved by preventing formation of the fibrin sheaths.

The invention provides a peripheral blood mononuclear cell cryopreservation solution, a preparation method and application of the peripheral blood mononuclear cell cryopreservation solution and a peripheral blood mononuclear cell cryopreservation method, and relates to the technical field of cells. The peripheral blood mononuclear cell cryopreservation liquid comprises the following components: a permeable protective agent, a non-permeable protective agent, human serum albumin, a 1640 basal culture medium, glutathione, autologous plasma, vitamin C and heparin sodium, wherein the autologous plasma is plasma of the peripheral blood mononuclear cells. According to the technical scheme, the permeable protective agent and the non-permeable protective agent are combined, and meanwhile, the human serum albumin, the 1640 basic culture medium, the heparin sodium, the glutathione, the autologous plasma and the vitamin C are added, so that a metabolic environment in the cell cryopreservation process is provided, and the motility rate and the proliferation activity of the recovered cells are ensured.

The utility model relates to the technical field of heparin sodium extraction, in particular to casing cleaning and extracting integrated equipment for extracting heparin sodium, which comprises a protective shell, the outer wall of the protective shell is fixedly connected with a first gear motor, and the inner wall of the protective shell is rotatably connected with a threaded rod. The output end of the first gear motor is fixedly connected with one end of a threaded rod, the rod wall of the threaded rod is sleeved with a first sliding block in a threaded mode, the lower end of the first sliding block is fixedly connected with a connecting plate, the lower end of the connecting plate is fixedly connected with an electric push rod, and the output end of the electric push rod is fixedly connected with a scraper. According to the heparin sodium extraction and collection device, large-particle substances on the filter screen plate are collected into the collection tank through the two collection plates, so that the filter screen plate can be effectively prevented from being blocked in the heparin sodium extraction and collection process, the filter screen plate is prevented from being frequently disassembled, the risk of equipment damage is reduced, and the service life of equipment is prolonged.

The invention relates to the technical field of medical instruments, in particular to a variable-diameter blood seepage prevention artificial blood vessel based on a multi-layer composite structure, which comprises a composite artificial blood vessel main body, and a bioactive composite layer is arranged on the outer side of a gradient pore polyester woven layer. The main body of the composite artificial blood vessel is composed of more units such as the first artificial blood vessel part, the second artificial blood vessel part, the third artificial blood vessel part and the fourth artificial blood vessel part, and the inner diameter difference between every two adjacent units is 2.0 mm, so that the variable-diameter structure is more suitable for natural blood vessels of people clinically; in addition, the bioactive composite layer comprises type I collagen, heparin sodium and VEGF sustained release microspheres, the collagen / heparin composite layer forms a hydrated gel barrier when in contact with blood, the errhysis volume is reduced to 1 / 16 of that of a traditional product, endothelial cell migration is promoted by utilizing TiOnanotube array layer and VEGF sustained release synergy, and in addition, under the pulsating pressure of 120 mmHg, through 1 million times of cycle tests, the effective rate of the collagen / heparin composite layer is greatly increased. The structural integrity retention rate is larger than 99%, and the mechanical fatigue resistance is high.

The utility model relates to the technical field of crude heparin sodium preparation, and provides an adsorption tank for crude heparin sodium preparation, which comprises an adsorption cylinder, the top of the adsorption cylinder is fixedly connected with a fixing frame, and the top of the fixing frame is fixedly connected with a first motor. Through the arrangement of the first motor, the rotating shaft, the rotating plate, the connecting rods and the clamping plates, when the first motor starts to operate, the rotating shaft drives the rotating plate to rotate, the rotating plate extrudes and pushes the two connecting rods, the two connecting rods drive the two clamping plates to horizontally move oppositely under the limiting action of the sliding grooves, and the clamping plates are clamped. And the clamping plate is separated from the interior of the handle, so that the fixing effect on the connecting plate is relieved, an operator can take down the connecting plate and the stirring blade through the handle, the function of conveniently disassembling and assembling the stirring blade is achieved, the efficiency of disassembling and assembling the stirring blade by the operator is improved, and the labor intensity of the operator is reduced.

The invention discloses a heparin sodium purification method and application thereof, and the purification method comprises the following steps: S1, enzymolysis pretreatment: adding an animal mucous membrane liquid into a reaction kettle, adjusting the pH value, and carrying out enzymolysis at the temperature; s2, ultrasonic-assisted treatment: applying ultrasonic waves to the enzymatic hydrolysate; s3, adding a protective agent, namely adding a monosaccharide or disaccharide protective agent; s4, purification with ion exchange resin: dynamically adsorbing the heparin sodium by using strongly basic anion exchange resin, and eluting; s5, ethanol precipitation and ultrafiltration: ethanol is added, heparin sodium is precipitated, and then micromolecular impurities are removed through ultrafiltration; and S6, freeze-drying and forming: freeze-drying to obtain a finished product of heparin sodium. The invention relates to the technical field of biological medicine. According to the purification method and application of the heparin sodium, efficient separation and purification of the heparin sodium are achieved through the synergistic effect of multiple steps of enzymolysis, ultrasonic-assisted treatment, protective agent stabilization, ion exchange resin purification, ethanol precipitation and the like, the titer and purity of the product are remarkably improved, and the purification method is suitable for industrial production.

The application discloses a processing equipment for crude heparin sodium and belongs to the field of heparin sodium preparation, which comprises a shell, a liquid discharge port arranged at the lower end of the shell, a cover one fixed at the upper end of the shell, a liquid inlet arranged on the cover one, and an adsorption assembly arranged in the shell. The adsorption assembly comprises a plurality of supporting pieces fixed to the inner bottom wall of the shell, a filter cartridge clamped to the upper ends of the supporting pieces, a cavity and a hollow cavity arranged in the filter cartridge, and a cover two fixed to the upper end of the filter cartridge and sealing the cavity. The cavity for placing the resin is arranged in the filter cartridge, the resin is confined in the filter cartridge, direct contact with the high-speed rotating stirring paddle is avoided, and the resin is not damaged by the high-speed rotating stirring paddle. The paddle in the hollow cavity can actively and continuously suck the liquid from the bottom of the filter cartridge, and force the liquid to penetrate the resin bed in the radial direction. The radial flow has a large cross-sectional area, a short path, and a more uniform fluid distribution, and the utilization rate of the resin is higher.

The invention discloses a human blood coagulation factor VIII and a preparation method thereof, and the preparation method comprises the following steps: S1, carrying out refrigerated centrifugation on cryoprecipitate to obtain an extracting solution; s2, mixing the extracting solution and the heparin sodium solution to obtain a dissolving solution; s3, adjusting the pH value of the dissolving solution to 6.2-6.4 by using hydrochloric acid, and controlling the temperature to be 20-30 DEG C to obtain an acid precipitate; s4, mixing the acid precipitate with polyethylene glycol to obtain a polyethylene glycol precipitate; s5, adding the carboxylated ferroferric oxide nano-particles into the polyethylene glycol precipitate, incubating, and carrying out magnetic separation to obtain a first separation solution; s6, performing centrifugal separation on the first separation liquid to obtain a second separation liquid; s7, clarifying and filtering the second separation liquid, and adjusting the pH value to 6.4-7.4 to obtain a clarified liquid; and S8, adding a stabilizer and an inactivator into the clear liquid, and carrying out water bath heat preservation to obtain a human blood coagulation factor VIII stock solution. The method has the effect of improving the recovery activity and purity of the human blood coagulation factor VIII.

The application discloses heparin sodium cream and a preparation method thereof, and belongs to the technical field of pharmaceutical preparation production. The heparin sodium cream comprises the following components: heparin sodium, propylene glycol, hydroxyphenyl ethyl ester, sodium dodecyl sulfate, glycerol monostearate and distearate, octadecanol, liquid paraffin, white vaseline and water. The preparation method comprises the following steps: preparing an oil phase and mixing an aqueous phase; taking part of the mixed aqueous phase and the oil phase to form a water-in-oil type primary emulsion; then adding the remaining mixed aqueous phase into the primary emulsion, inducing phase transition into an oil-in-water type cream under mild conditions, and obtaining the finished product after vacuum defoaming and gradient cooling. The application realizes high-efficiency emulsification under mild conditions below 80 DEG C and 3000 rpm through a controlled phase inversion emulsification process, so that the particle size (D99) of the emulsion droplets is less than 5 microns, and the problem that the active substance heparin sodium loses activity under severe conditions is solved completely, and the stability of the product is improved significantly.

The invention discloses a production device for extracting sodium heparin by utilizing pig small intestinal mucosa, comprising a feeding roller, a sleeve rod and a base, wherein the sleeve rod comprises an upper sleeve rod and a lower sleeve rod connected to the lower part of the upper sleeve rod, a deep hole 1 cooperating with the upper sleeve rod is provided on the upper part of the base, a deep hole 2 cooperating with the lower sleeve rod is provided at the bottom of the deep hole 1, the deep hole 2 passes through the lower side surface of the base, the upper sleeve rod and the lower sleeve rod are respectively located in the deep hole 1 and the deep hole 2, and an annular scraper is arranged on the outer wall of the upper sleeve rod. The invention can scrape off the mucosa in a long section of the small intestine, and at the same time does not affect the subsequent production of the small intestine as casing during the processing.

The utility model relates to the technical field of heparin sodium, in particular to a heparin sodium production wastewater treatment device, and solves the problems that in the prior art, a filter screen cannot be cleaned, impurities on the filter screen block the filter screen, the impurities cannot be collected into filter cloth, and the filter cloth cannot be detached. The heparin sodium production wastewater treatment device comprises a placement plate, an outer cylinder arranged at the center of the top of the placement plate and a driving assembly arranged at the bottom of the placement plate, an inner cylinder is arranged in the outer cylinder, a stirring structure is arranged in the inner cylinder, one end of the stirring structure penetrates through the inner cylinder and the outer cylinder through a shaft sleeve, and an output shaft of the driving assembly is connected with the end of the stirring structure. Impurities can be conveniently blocked and collected through the multiple filter plates, then the filter plates are taken out from the interior of the through groove, the filter plates can be rapidly cleaned or replaced, and the filter screen is prevented from being blocked.

The utility model discloses a salt feeding device for heparin sodium, which relates to the technical field of organic chemical processing equipment, solves the technical problem that quantitative feeding and uniform stirring of raw materials cannot be realized in the prior art, and comprises a main base, and a liquid feeding structure is fixedly mounted on the upper wall surface of the main base. The salt conveying structure comprises a main base, a salt conveying pipe is fixedly installed on the upper wall face of the main base, a main shaft is rotationally installed in the salt conveying pipe, auger blades are fixedly installed on the main shaft, a salt conveying driving structure is arranged on the salt conveying pipe, and a salt storage bin is fixedly installed on the main shaft and the main base. Through the stirring effect of the auger blades, quantitative and uniform conveying of salt materials can be achieved, the salt hydrolysis efficiency is improved, the quality and stability of final products are ensured, the liquid storage pipe can assist in adding a needed solution in the salt hydrolysis process, the salt storage bin can store a large number of raw materials, the number of manual operation times is reduced, and the production efficiency is improved.

The invention relates to the technical field of crude heparin sodium processing, in particular to crude heparin sodium impurity removal and purification equipment which comprises a system frame and an impurity removal and purification system erected on the system frame. A primary screening compartment is arranged at the feeding end of the impurity removal system, and double-layer staggered rotary crushing rollers are arranged in the primary screening compartment; the turnover flow guide box is provided with a hydraulic drive spiral flow guide plate; the filtering device comprises upper-layer transverse stainless steel filtering wires and lower-layer longitudinal ceramic filtering wires; the crystallizer is provided with a gradient cooling condensation module. The purification system comprises a centrifugal separator, a preheating guide cylinder and a dryer, the centrifugal separator is in butt joint with the crystallizer through a purification guide-in box, the bottom of the centrifugal separator is connected with the preheating guide cylinder through a heparin sodium guide-out cylinder, a steam jacket and a spiral blade are arranged in the centrifugal separator, the outlet end of the centrifugal separator is connected with the dryer, and a vacuumizing assembly is integrated on the side edge of the dryer. Through integrated design of crushing, crystallization, preheating and intelligent control, efficient impurity removal and purification of the crude heparin sodium are realized, and the product purity and the production efficiency are improved.

The utility model relates to the technical field of wastewater treatment equipment, and discloses a waste liquid treatment device for heparin sodium production, which comprises a treatment pond, a purification box is slidably connected to the inner wall of the treatment pond, a filter plate is detachably connected to the inner wall of the purification box, an air cylinder is mounted outside the treatment pond, and the filter plate is detachably connected to the inner wall of the purification box. A linkage plate is rotationally connected to the inner wall of the purification box, a rotating block is rotationally connected to the inner wall of the linkage plate, the driving end of the air cylinder is fixedly connected to the bottom of the rotating block, a linkage rod is movably connected to the inner wall of the purification box, and the other end of the linkage rod is movably connected to the inner wall of the linkage plate. According to the waste liquid treatment device, the linkage rod ascends and descends to drive the purification box and the filter plate to ascend and descend, so that an operator can conveniently replace purification substances in the purification box, and the device allows purified water to be separated more easily after waste liquid treatment and ensures that the treated water is purer.

The invention provides a method for 3D differentiation of pluripotent stem cells into definitive endoderm cells by using a bioreactor, a differentiation medium and a differentiation factor A / B are also included, and the medium and the differentiation factor A / B comprise the following components: an MCDB131 medium, 2.44 mM glucose, 29.28 mM sodium bicarbonate, 2% of HSA, 1% of GlutaMAX, 250 [mu] M Vitamin C, 0.002% of ITS-X, 1% w / v of Polyethylene glycol (PEG), 1 mg / ml of Heparin Sodium salt, 100 ng / mL of Activin A and 3 [mu] M of CHIR99021. The method uses the bioreactor to differentiate the pluripotent stem cells into the stereotyped endoderm cells in a 3D suspension manner, is simple to operate and high in differentiation efficiency, provides technical support for scientific research and industrial large-scale production, and has a good application prospect.