1511 results about "Salting out" patented technology

The present invention provides a method for comprehensively treating high salt content organic industrial wastewater, and relates to the field of the organic material preparing technique. The method of the invention mainly comprises the following steps: executing acid-alkali adjustment to the organic wastewater until pH=7-9, preheating to 30-60 DEG C; introducing the wastewater into a triple effect evaporator for executing triple effect evaporation, executing salting out to the evaporated wastewater which is condensed to a certain degree so that the solid and liquid are separated; separating the concentrated liquid for continuing the condensation; when the separated liquid satisfies a certain requirement, atomizing into an incinerator for incinerating and discharging according to the standard, and introducing the triple effect evaporation condensation water into a biochemical treating system. The method of the invention can totally dispose and remove the organic matter in the high salt content organic industrial wastewater so that the high salt content organic industrial wastewater can be charged according to the standard. The method of the invention has the advantages of unique method, simple technical process, easy operation, low operation cost, large treating capacity, low regeneration energy consumption, no easy forming of secondary pollution after abandon, better economic and social benefit, and wide application range. The method of the invention can be widely applied for the treating of the high salt content organic wastewaters of garbage penetrating fluid, dye intermediate wastewater, etc.

The invention relates to a clean production process of plateau sulfate type boron-lithium salt lake brine. The process comprises the following steps of: (1) arranging a pre-airing pond, a mirabilite pond, a NaCl pond, a carnallite pond, an epsom salt pond I, a magnesium removing pond, an epsom salt pond II, a boron pond, a lithium pond and an old brine pond; (2) controlling the sodium ion concentration in plateau sulfate type boron-lithium salt lake brine, precipitating mirabilite out in winter to obtain brine A, naturally evaporating the brine A, and salting out to obtain brine B; (3) naturally evaporating the brine B, and precipitating sylvine and carnallite out in sequence to obtain brine C; (4) naturally evaporating the brine C, precipitating an epsom salt out, and performing solid-liquid separation to obtain brine D and a solid A; (5) blending the brine D with mirabilite, removing magnesium to obtain brine E, and naturally evaporating brine E to obtain brine F and a solid B; (6) performing a hydration reaction on brine F, naturally evaporating, and precipitating reservoir water/inderite and brine G out; and (7) evaporating brine G or refrigerating for precipitating lithium sulfate, and processing the lithium sulfate into a corresponding product. The process has the advantages of comprehensive utilization of natural energy, saving in energy and environment friendliness.

The invention provides a preparation method of high-purity collagen protein sponge, and relates to a preparation method of collagen protein sponge. The preparation method of the high-purity collagen protein sponge is used for solving the problems that the collagen protein sponge prepared by using a conventional method is long in production cycle and low in yield and purity and has poor hemostasis performance. The preparation method of the high-purity collagen protein sponge comprises the following steps: step one. pretreating fresh bovine heel tendons; step two. extracting collagen protein; step three. centrifuging; step four. salting out; step five. dissolving; step six. carrying out gradient dialysis; step seven. pre-freezing; step eight. carrying out freeze drying; and step nine. sterilizing. The final product prepared by using the method has a smooth and flat surface and relatively good hemostatic performance and is uniform in pore size distribution. The product has relatively high purity (the total amount of amino acids reaches 97.73%), an obvious hemostatic effect and no abnormal taste, is safe, non-toxic, high in yield and short in time; liquid is clear without impurities; the production cycle is shortened; the whole preparation process is carried out at a room temperature; the biological activity of the collagen protein is maintained; and the application of the high-purity collagen protein sponge in clinical is improved.

The invention discloses a method for separating and purifying DHA (docosahexaenoic acid) and saturated fatty acid from schizochytrium limacinum oil. The method includes: firstly, saponifying, salting out and acidizing the schizochytrium limacinum oil under protection of nitrogen so as to obtain free mixed fatty acid, and separating the fatty acid different in degree of saturation by urea adduction fractionation so as to obtain filtrate and solids after filtration; concentrating and extracting the filtrate so as to obtain polyunsaturated fatty acid rich in DHA and DPA (docosapentenoic acid); and allowing the solids to leach by means of acidolysis, extracting the saturated fatty acid (mainly comprising palmic acid) and recovering urea, wherein the urea is recyclable. The method is performedat a low temperature, oxidization of the unsaturated fatty acid is avoided, biological activity and nutrition of the unsaturated fatty acid are kept intact, and the problem of residual solvent is avoided. Products are high in purity, and the obtained polyunsaturated fatty acid mainly comprises the DHA and the DPA and hardly comprises EPA (eicosapentaenoic acid), the content of the DHA and the DPAis higher than 93%, and the content of the palmic acid of the saturated fatty acid is higher than 82%.

The invention provides a method for improving the quality of expanded cut rolled stem of tobacco by microbial enzyme. The procedures are as follows:1) Preparation of bio-enzyme: (1) Aspergillus niger is activated and cultured by potato cane sugar culture medium and is then transferred into sterilized seed culture medium, and shake cultivation is carried out at the temperature of 28 DEG C for 24 hours with 250r/min<-1>; seed liquid with 10% of inoculation amount is transferred into a sterilized 500ml shake flask (containing 100ml culture liquid for enzyme), and the seed liquid carries out incubation for culturing at the temperature of 25 DEG C for 96 hours and then zymotic liquid is obtained. (2) Extraction of lignin degradation complex enzyme: After being filtered by filter paper, the zymotic liquid is centrifugally separated at the low speed of 1500rpm. The supernatant carries out fractional salting out by (NH4)2SO4 till the saturation is 0.7 to obtain crude enzyme liquid, after the crude enzyme liquid carries out ultrafiltration dialysis, the lignin degradation complex enzyme is obtained. Activities of enzyme components: 350U/L of Lip, 11U/L of MnP and 5.6U/L of Lac; 2) Improvement of the quality of expanded cut rolled stem of tobacco: The expanded cut rolled stem for cigarette is weighed and sprayed evenly by lignin degradation complex enzyme liquid containing 0.2-0.4% of expanded cut rolled stem after being diluted by distilled water at the temperature of 27 DEG C-29 DEG C. The expanded cut rolled stem of tobacco is placed in a sealed container for enzymolysis for 95-97 hours and dried naturally till the moisture content is 12.5%-15%. The result by subjective analysis suggests that the complex enzyme preparation can effectively decrease the lignin content of the expanded cut rolled stem, promote the transformation of aroma matter and improve aroma quality and taste.

A process for preparing the collagen sponge includes such steps as extracting collagen in acetic acid by digestion of pepsinum, filtering, taking supernatant, neutralizing by sodium hydroxide, salting out in sodium chloride solution, centrifugal separation of deposit, dissolving it in acetic acid, dialyzing by acetic acid and then by the solution of bisodium hydrogen phosphate, and freeze drying.

The invention belongs to the technical field of bioengineering, and relates to a method for extracting and salting organic acids out of fermentation liquor. The method is characterized in that: soluble inorganic or organic salt solids or concentrated solution thereof and an extracting agent are added into the organic acid fermentation liquor, stirred for uniform mixing, and stood at room temperature for phase splitting; when two phases are formed by the salting-out and extraction, an upper phase is a solvent phase rich in the organic acids, while a lower phase is a salt phase rich in other components; if the extraction operation is directly performed without pretreating the fermentation liquor, a solid phase layer rich in bacteria and proteins is formed between the two phases; when three phases are formed, the upper phase is the solvent phase, a middle phase is the phase rich in the organic acids and the lower phase is the salt phase rich in the other components; therefore, the aim of extracting and separating the organic acids is fulfilled. The method solves the problems of difficulties in product separation, high cost and the like in the conventional process for separating the organic acids by a fermentation method, is simple, ensures separation time, low cost and high recovery rate, and is an organic acid separation method with industrial application prospect.

The invention relates to the filed of microalgae utilization engineering, in particular to a production method for fully utilizing oleaginous microalgae. The method is characterized in that the wall of the microalgae is broken by a high-pressure homogenization method, and the solid phase and liquid phase are separated out by an extraction kettle and a horizontal spiral centrifuge, and microalgae powder is made from the solid phase by a spray dryer. An oil phase and a water phase are separated from the liquid phase by an oil-water separator, and the oil phase is microalgae oil. A solid phase and a liquid phase are separated from the water phase by a vacuum concentration kettle, a salting-out kettle and a high-speed centrifugal filter, and microalgae protein is made from the solid phase by a freeze dryer or a spray dryer. A solid phase and a liquid phase are separated from the liquid phase by an alcohol precipitation kettle and the high-speed centrifugal filter, and microalgae polysaccharide is made from the solid phase by the freeze dryer or the spray dryer, and ethanol and microalgae liquid fertilizer are made from the liquid phase by an ethanol recovery tower. The invention is a good production method which requires low investment, has simple operation and can comprehensively use microalgae on a large scale, and can produce semi-finished products with high added value, such as the microalgae oil, the microalgae powder, the microalgae protein, microalgae polysaccharide, the microalgae liquid fertilizer, etc.

The invention relates to a method for preparing fish scale collagen. The method comprises the following steps: fresh fish scales are taken as a material, undergo cleaning, drying and crushing, are soaked in NaOH solution for degreasing, and soaked in hydrochloric acid solution for deashing; the fresh fish scale is added with water of which the weight is 5 to 10 times of that of the fish scales, the pH value is adjusted to between 2 and 4 by acid solution, pepsin of which the weight accounting for the weight of the mixing solution is between 1 and 5 percent is added, the mixing solution undergoes enzymolysis at a low temperature for 4 to 10 hours, and enzyme is killed; enzymatic liquid is filtered twice to obtain extracting solutions, the extracting solutions are mixed, the mixing solution is decolored, sodium chloride of which the weight accounting for the weight of the mixing solution is between 5 and 15 percent is added into the mixing solution for overnight salting out, deposit is separated and collected, and acetic acid of which the weight accounting for the weight of the mixing solution is between 5 and 20 percent is added into the deposit for dissolution to obtain protein solution; the protein solution is dialyzed and desalted to obtain purified collagen liquid; and the collagen liquid is subjected to freeze-drying or spray-drying and micro-crushing to obtain solid particle. The method has the advantages of reasonable process, simple operation, safety, low cost, little loss of nutrient matters of the product, low ash content and high yield and purity of the collagen.

The invention discloses a method for preparing collagen peptide rich in hydroxyproline. The method comprises the following steps: firstly, extracting collagen from anglerfish as a raw material by using an acid liquid, subsequently performing salting-out and dialysis purification, hydrolyzing fishskin collagen by using pepsase and flavourzyme under the condition of ultrasonic wave in different steps, removing the fragrance and the color, performing centrifugal separation, and subsequently freeze-drying so as to obtain the collagen peptide rich in hydroxyproline. By adopting the method, waste of anglerfish skin is effectively utilized, and the collagen peptide rich in hydroxyproline is prepared by hydrolyzing anglerfish skin, so that not only is high nutrition value of the collagen peptide extracted from the anglerfish skin utilized, but also the problems that the waste of the anglerfish skin pollutes the environment and the resource is wasted are solved. The method for preparing the collagen peptide rich in hydroxyproline provided by the invention not only is applicable to a fish skin raw material, but also can be applicable to other raw materials, so that the method has a good application prospect.

The invention relates to an aqueous enzymatic method for extracting rapeseed oil and recovering protein. The aqueous enzymatic method comprises following steps: raw material rapeseed is crushed, immersed in an acid solution at a high temperature (80 to 120 DEG C) for 0.1 to 2h, and grinded to obtain a pulp; the pulp is filtered so as to remove shells; the filtrate is subjected to enzymolysis for 2 to 6h at a temperature of 30 to 60 DEG C by adding 50-500U/g of composite cellulose, then 100-500U/g of protease and a composite enzyme of protease is added, and the mixture is subjected to enzymolysis for another 2 to 6h at a temperature of 30 to 60 DEG C; the mixture is filtered, the obtained residue can be used as forage directly after drying, and the obtained liquid phase is subjected to emulsion breaking, wherein emulsion breaking is realized by three methods; after emulsion breaking, the liquid phase is filtered to obtain liquid content and solid content; the solid content is subjected to spray drying so as to obtain rapeseed protein; and the liquid content is filtered so as to obtain rapeseed oil and a water phase, and the water phase is treated with a membrane so as to obtain soluble protein. The three methods of emulsion breaking comprise: A, a food flocculating agent is added for emulsion breaking; B, concentrated sulfuric acid or sodium hydroxide solution is added to adjust the pH value to the isoelectric point of the liquid phase for emulsion breaking; and C, sodium chloride solid is added for salting out emulsion breaking. Recovery rate and quality of the rapeseed oil and protein obtained by the aqueous enzymatic method are high, and no organic solvent is used in the whole process of the aqueous enzymatic method. Rapeseed is immersed in the acid solution before enzymolysis, so that the generation of antinutritional factors is avoided. Processing equipment required by the aqueous enzymatic method is simple, and cost is low.

An object of the present invention is to provide an immunoassay of PSA using an agglutination accelerator, which has an agglutination accelerating effect equal to or stronger than the known agglutination accelerator; hardly generates non-specific turbidity; and hardly generates salting out even in a solution with a high salt concentration. The present invention relates to an immunoassay of a prostate-specific antigen comprising performing an antigen-antibody reaction in the presence of a polymer having a monomer unit derived from a monomer represented by the following general formula [2]: (wherein R<1>-R<3 >are each independently a hydrogen atom or an alkyl group optionally having a hydroxyl group; R<4 >is an alkylene group; R<5 >is an alkylene group optionally having a substituent and optionally having an oxygen atom in a chain; R<6 >is a hydrogen atom or a methyl group, and X is an oxygen atom or a -NH- group), and a kit of reagent for an immunoassay comprising a reagent containing an agglutination accelerator for the immunoassay.

The invention discloses a method of extracting non-denaturing natural collagen from the animal skin or / and the tendon, and the method comprises the following steps the raw material chips of the animal skin or / and the tendon in which the lipa and the impurity are removed, and which is soaked and per-processed through acetone is added into a reactor and soaked with acetate solution, stomach cnzyme is added after full soaking, the enzymolysis is performed under the irradiation of the ultrasonic wave, after enzymolysis, enzymolysis liquid is diluted with the acetate solution, the part that is not completely enzyme-decomposed is separated and removed in the enzymolysis liquid, obtained collagen aqueous solution is salted out with 2 mol/L NaCl or (NH4)2SO4 solution, collagen salted object obtained by separating and salting out suspended material is stirred and dialyzed with distilled water, and the product after being dialyzed is frozen out through the freezing dryer, namely, the collogen product is obtained. By adopting the method of the invention to prepare the collogen, the extraction time of the collogen can be greatly saved, the collogen production rate is improved, and the obtained collogen can completely retain the three unique spiral structures, which can be applied to the biological medicine material.

The invention provides an UPLC-MS/MS method for analyzing four categories and 29 types of restricted veterinary drug residues in animal foodstuff. The method is characterized in that an advanced QuEChERS method is adopted for conducting sample preprocessing, a sample is subjected to McIlvaine buffering liquid-acetonitrile extraction, an extracting solution is subjected to acidification and salting out, a small amount of acetonitrile phase is taken, a C18 and NH2 adsorbing agent is utilized for conducting dispersive solid-phase extraction and purification, after purification liquid is subjected to concentration and volume setting, an ultra-high performance liquid chromatography-tandem mass spectrometer (UPLC-MS/MS) is adopted to conduct detection. The problem that it is difficult for tetracycline class veterinary drug to be extracted and purified along with other types of veterinary drugs is solved, meanwhile the sample processing steps are simplified, the UPLC-MS/MS method has the advantages of being efficient, rapid, low in cost, simple and convenient to operate, good in purification effect and high in sensitivity, and the accuracy and precision both meet the requirement of a multi-residue analysis method.

A process for extracting RE from the RE contained phosphorite is disclosed, which features that its immersing liquid is nitric acid, its salting-out agent is ammonium (or sodium) nitrate, its extractant is dimethylheptyl methylphosphonate (P350) or 2-ethylhexyl phosphonate-bis(2-ethylhexyl) ester (P502), its diluent is n-heptane or kerosene, its washing liquid is the solution of sodium (or ammonium) nitrate, its backextractant is the solution of nitric acid or hydrochloric acid, and its preciptant is oxalic acid. Its advantage is high purity (more than 95%) and high output rate (more than 98%) of RE.

The invention relates to an extraction method for bioactive collagen. The method comprises: a pretreatment step, i.e. pretreating fresh bovine tendon into fragments for standby use; an acid process crude extraction step, i.e. taking 1 part by weight of fragment, adding 4-8 parts by weight of an acetic acid or citric acid solution and conducting stirring, then carrying out coarse filtration, performing high speed centrifugation on a coarse filtrate, then taking the supernatant for standby use; a step of end group removal by an enzymatic process, i.e. adjusting the pH of the supernatant to 9.0, then adding 0.1-0.3% part by weight of Ficus proteinase to conduct enzymolysis digestion; a gradient salting-out step, i.e. using a precipitating agent to conduct multi-gradient salting-out treatment on the solution subjected to enzymolysis to obtain a collagen dissolved solution; and a dialysis step, i.e. loading the collagen dissolved solution into a dialysis bag, using an acetic acid solution and pure water as outer dialysate in order to conduct dialysis, thus obtaining a collagen stock solution with bioactivity. The method provided by the invention combines two existing collagen extraction processes, i.e. the acid process and the enzymatic process, overcomes the low yield defect of the acid process and the incomplete hydrolysis defect of the enzymatic process, and adopts non-animal derived protease, thus reducing the risk of animal origin.

The invention relates to the field of marine active substance preparation from aquatic animal processing byproduct sources, and aims to provide a simple and quick high-extraction-rate comprehensive utilization technique of marine fish bones. The technique provided by the invention comprises the following steps: processing the raw material, crushing the fish bones, extracting chondroitin sulfate, carrying out enzymolysis on the filtrate to remove proteins, inactivating enzymes at high temperature, precipitating the chondroitin sulfate, removing fat from the filter residues, extracting collagen, salting out the collagen, collecting collagen, dialyzing the collagen, and carrying out freeze-drying and the like. The invention is simple and quick, has the advantage of high extraction rate, and achieves the goal of simultaneously producing chondroitin sulfate and collagen.

The invention discloses a common rapid detection method for various pesticide residues in soybeans. The common rapid detection method is characterized by particularly comprising the following steps: homogenizing and extracting a soybean sample by acetonitrile; carrying out salting-out and centrifuging to obtain a supernatant; purifying by using a solid phase extraction column to obtain a sampling solution; preparing a mixed standard solution and preparing a base material adding standard curve; simultaneously detecting 306 types of pesticide residues in the soybeans by a gas chromatography-tandem mass spectrometer; finally, calculating the concentration. The common rapid detection method has the advantages that the effect of the base material and background interferences are reduced and the anti-interference capability of the method is improved greatly; the analysis sensitivity is improved; the detection linear range is 0-0.2microgram/milliliter; the detection is limited to be 0.01mg/kg; the recycling rate is 60%-120%; the relative standard deviation RSD is less than or equal to 20%; the recycling rate is ideal and the precision degree is good; the common rapid detection method is simple and rapid, is low in background interferences, good in selectivity and high in sensitivity.

The invention relates to a process for preparing amino G acid, which belongs to the technical field of amino K acid production process. The process comprises the steps of sulfonating, salting out by ammoniacal liquor, treating G salt mother liquor, ammoniating G salt ammonium base sulfite, treating dehydrated liquid and the like, ammonium base sulfite is employed as a catalyst for replacing ammonium bisulfite, so that the pressure generated during ammonification process can be effectively reduced, the reaction time can be shortened, and the product yield and content can be increased. The method is characterized in that two streams of waste water are respectively treated, 2-naphthol is hydrolyzed and recovered by G salt mother liquor, neutralized for reaching the standard, and then exhausted; ammonium sulfate-rich effluent is used for producing fertilizer, and the zero discharge is realized. The unit consumption of the raw material 2-naphthol is decreased from 0.75 ton/ton in a traditional process to less than 0.64 ton/ton when the problem of waste water treatment is solved, so that the production cost is reduced, the production safety coefficient is high and no harmful gas is discharged. The process of the invention solves the problems that two streams of waste water are generated during the present production process, the difficulty of waste water treatment is large, the production cost is high and the large harm on environment is caused.