33 results about "Collagen sponge" patented technology

The invention relates to the technical field of biomedical materials, in particular to collagen sponge and a preparation method thereof.The preparation method comprises the following steps that under the condition of 4-8 DEG C, silver-containing turbid liquid is added into a collagen solution, ultrasonic treatment is conducted, magnetic stirring is matched at the same time, and a mixed solution is obtained; performing high-pressure homogenizing and refining treatment on the mixed solution to obtain a homogenized solution; freezing the homogenized liquid at-80 DEG C, and then unfreezing at 4-37 DEG C to obtain pretreated homogenized liquid; and carrying out freeze drying on the pretreated homogeneous liquid to obtain the collagen sponge. According to the preparation method disclosed by the invention, a low-temperature ultrasonic-magnetic composite dispersion method is combined with homogenizing treatment, so that silver salt is uniformly dispersed in a collagen solution on the premise of avoiding thermal denaturation of collagen; and then a freezing-unfreezing pretreatment process is combined, so that the hydrogen-bond interaction between the silver salt uniformly dispersed in the collagen solution and the collagen is enhanced, the binding force between the silver salt and the collagen is improved, and sudden release of the silver salt is avoided.

The present invention relates to an I-type recombinant collagen with high transdermal absorbability, and the use thereof. The human I-type recombinant collagen with high transdermal absorbability of the present invention is composed of multiple repetitions of a short amino acid sequence from natural human I-type collagen as a repeating unit, wherein the short amino acid sequence is as shown in SEQ ID NO: 1, and the number of repetitions is three or more. The human I-type recombinant collagen with high transdermal absorbability of the present invention can be used as a raw material for producing various collagen products, such as an injection, a facial filler, a dressing, cosmetics, a health-care food product, tissue engineering material and a collagen sponge.

The invention discloses a novel absorbable wound dressing and a preparation method thereof. The novel absorbable wound dressing is prepared from collagen, acellular extracellular matrix powder and purified water. The novel absorbable wound dressing with activity is prepared by compounding the acellular extracellular matrix powder and the collagen, the acellular extracellular matrix powder contains various growth factors, various growth factors do not need to be additionally added, and compared with a common collagen sponge dressing, the novel absorbable wound dressing has the better effect of promoting wound healing, and the wound healing effect is better. Meanwhile, collagen is directly adopted as a hemostasis carrier of the acellular extracellular matrix powder and is subjected to freeze-drying forming, seepage of the wound surface can be absorbed, the mechanical strength of the acellular extracellular matrix powder can be enhanced, and therefore the wound can be rapidly and effectively bonded, and the hemostasis effect is achieved. The safety is high; and the manufacturing method is simple.

This invention provides a novel method for preparing a medical collagen sponge, relating to the field of medical sponge technology. The invention uses yeast extract obtained by Soxhlet extraction of brewer's yeast as a raw material, which is mixed and reacted with polyvinyl alcohol and graphene oxide to obtain the novel medical collagen sponge. The medical collagen sponge prepared by this invention exhibits excellent performance and good hemostatic effect. Furthermore, the preparation method is simple, and it degrades postoperatively, demonstrating significant application value in the field of medical materials.

The invention relates to the technical field of biomedical materials, in particular to silver-containing collagen sponge and a preparation method thereof.The preparation method comprises the following steps that S1, silver salt is added into a collagen solution, and a first solution is obtained; s2, glutathione and ethylene diamine tetraacetic acid disodium salt are added into the first solution, and a second solution is obtained; s3, adding sodium alginate and chitosan into the second solution, and homogenizing to obtain a homogenized solution; and S4, carrying out freeze drying on the homogenized liquid to obtain the silver-containing collagen sponge. The preparation method of the silver-containing collagen sponge provided by the invention is simple in process, good in reproducibility and suitable for large-scale production; according to the silver-containing collagen sponge, chelating is stably combined with a microcapsule coating composite system, oxygen is isolated from silver ions, the oxidation rate of the silver ions is remarkably reduced, meanwhile, slow release of silver is achieved, and therefore the antibacterial time efficiency of the silver-containing collagen sponge can be prolonged.

According to the preparation method of the moisturizing wound protecting membrane, the wound protecting membrane is of a double-layer structure and comprises a polyurethane outer membrane and a composite membrane composed of collagen sponge and an animal intestine membrane, and the preparation method of the moisturizing wound protecting membrane comprises the following steps that S1, the polyurethane outer membrane is prepared; s2, preparing a film layer; s3, preparing a collagen layer; s4, freeze-drying and molding the collagen layer and the membrane layer in a mold to obtain a collagen membrane; and S5, bonding the obtained polyurethane outer membrane with a collagen membrane to prepare the double-layer wound protecting membrane. The wound protecting membrane provided by the invention adopts a design of tightly combining three layers, the acellular matrix middle layer can be gradually degraded to inhibit scar hyperplasia, the polyurethane outer membrane provides a physical barrier and promotes cell adhesion and wound healing, and the three-dimensional porous collagen sponge bracket accelerates vascularization and cell ingrowth, so that the wound protecting membrane has a good wound healing effect. A novel decellularization technology is integrally adopted to retain biological activity, and an environment beneficial to skin growth is constructed through a double-layer structure.

The present application relates to a kind of composite piezoelectric negative pressure dressings and its preparation method, belong to medical dressing field.The present application includes: step S1: collagen is dissolved in acetic acid to obtain collagen solution, collagen sponge is obtained by freeze-drying process;Step S2: PVDF powder and ZnO nanoparticles are dissolved in organic solvent to obtain PVDF / ZnO solution;Step S3: the injector equipped with PVDF / ZnO solution is placed in electrospinning machine, collagen sponge is fixed on the collection wheel of electrospinning machine, and PVDF / ZnO nanofiber is covered on collagen sponge to form composite piezoelectric dressing by electrospinning method;Step S4: semipermeable membrane is covered on the top layer of composite piezoelectric dressing, while connecting drainage tube, forming negative pressure system, to obtain composite piezoelectric negative pressure dressing.The present application has good biocompatibility.Nanofiber membrane is more sensitive, and controllable negative pressure intensity makes it have excellent stability, and piezoelectric performance is more excellent.

The invention relates to the technical field of medical apparatus and instruments, in particular to a blood vessel puncture plugging device which comprises a plugging piece which is in a form of being attached to a blood vessel wall after being unfolded; one end of the suture is connected with the plugging sheet, and the other end penetrates through the puncture hole and then is arranged on the outer side of the blood vessel wall; the collagen sponge penetrates through the suture and is attached to the outer side of the blood vessel wall; the lock catch is arranged on the suture in a sleeving mode and used for locking the collagen sponge so that the plugging piece can be tightly attached to and cover the puncture position of the blood vessel wall; the face, facing the blood vessel wall, of the plugging piece is provided with micro-needles arranged in a protruding mode, and the micro-needles penetrate into the blood vessel wall when the plugging piece is attached to the blood vessel wall. Compared with the prior art, by arranging the microneedles on the plugging sheet and by means of the mode that the microneedles pierce the inner wall of the blood vessel, mechanical fixing force is provided, sliding of the plugging sheet is prevented, and then the reliability of puncture plugging is improved.

The invention provides oxidized sanguisorba officinalis cross-linked collagen hemostatic sponge and a preparation method thereof.The preparation method comprises the steps that firstly, sanguisorba officinalis extract is oxidized with sodium periodate, dialyzed and freeze-dried into powder, then the powder is dissolved with an acetic acid solution, a collagen solution is dropwise added, dialyzed and freeze-dried, and an oxidized sanguisorba officinalis cross-linked collagen sponge product is obtained; due to the introduction of the oxidized sanguisorba officinalis extract, the blood coagulation effect of collagen is effectively promoted, the endogenous blood coagulation effect of collagen can be remarkably improved, and the collagen has the capacity of inducing cell adhesion, proliferation and growth and meets the use requirements of medical materials.

The invention discloses a shapeable bone repair material capable of being implanted in a positioning manner and a preparation method thereof. The interior of the bone repair material is a core layer made of a gelatin, gallic acid and hydroxyapatite particle composite material, and the exterior of the bone repair material is a porous spongy collagen wrapping layer; the density of the wrapping layer is 20-100mg / cm < 3 >; the density of the core layer is 100-800 mg / cm < 3 >; and the particle size of the hydroxyapatite particles is 0.1-2 mm. The collagen sponge on the outer layer of the material wraps the gelatin / hydroxyapatite composite material, so that the problems of loose structure, weak binding force and displacement and powder falling after implantation of bone meal or collagen bone meal composite materials are solved, stimulation and trauma of bone meal particles to surrounding tissues can be avoided, and the conditions of bone meal discharge, bone grafting part collapse and the like are avoided. And in the self tissue regeneration process, the collagen component is degraded and absorbed, and the hydroxyapatite is fused with the new bone, so that bone regeneration is realized, and the success rate of tooth implantation can be improved.

This invention provides a platelet-rich plasma (PRP) sustained-release collagen sponge scaffold and its preparation method. The scaffold comprises: a first collagen sponge layer, a porous collagen sponge layer, and a second collagen sponge layer stacked sequentially; the porosity of the first and second collagen sponge layers is no more than 40%, and the pore size is no greater than 20 μm; the porosity of the porous collagen sponge layer is no less than 70%, and the pore size is no less than 50 μm; at least one sustained-release cavity is disposed in the porous collagen sponge layer for loading platelet-rich plasma gel. Through the structural design of the gradient pores of the collagen sponge and the internal sustained-release cavity, the outer layer forms a physical barrier to effectively prevent PRP loss; the inner layer efficiently stores PRP, ensuring the loading of effective components; the high porosity of the inner layer allows the PRP in the sustained-release cavity to slowly permeate outward, achieving long-term sustained release of PRP growth factors; at the same time, the pores of the inner layer can also provide sufficient space for cell migration and proliferation, promoting tissue regeneration.

The application discloses a kind of collagen Pickering emulsion 3D printing-based cartilage regeneration scaffold and preparation method thereof.Collagen is extracted from fresh collagen extraction raw material, and purified collagen sponge is obtained, the collagen sponge is dissolved in acetic acid, and high-concentration collagen solution is obtained, then oil phase is added to the collagen solution, and high-speed emulsification is carried out using high-speed homogenizer, to obtain collagen-based Pickering emulsion, finally, computer digital modeling and low-temperature hydrogel forming equipment are used, and collagen-based Pickering emulsion is used as biomaterial ink to 3D print cartilage regeneration hydrogel scaffold, the printed scaffold is quickly frozen in liquid nitrogen, and vacuum drying is carried out, to obtain cartilage regeneration scaffold.The application develops collagen Pickering emulsion biomaterial ink with excellent printability, and through 3D printing, collagen-based cartilage regeneration scaffold with good quick customization forming property, adjustable pore structure and good biological activity is obtained.

The present invention relates to a coating optimized drug delivery enhancement (COD2E; Dopa-fucoidan / PPL) system composed of dopamine-functionalized fucoidan and poly-lysine to facilitate secretome delivery, wherein the introduction of dopamine enhanced the adhesive strength by at least a factor of seven compared to existing coacervates (fucoidan / PLL), thereby imparting the ability to rapidly (5 minutes) and uniformly coat collagen sponges. The COD2E system was able to encapsulate fibroblast growth factor (FGF2) and extend the half-life of FGF2. In addition, biocompatibility, improved stability, antioxidant properties, and blood compatibility were demonstrated through in vitro and in vivo experiments, and thus the present invention may be useful as a regeneration accelerating agent due to having improved bioactivity and antioxidant effects.

The present disclosure provides a recombinant collagen and a recombinant collagen sponge material. The recombinant collagen comprises: (a) a protein composed of the amino acid sequence represented by SEQ ID NO: 2; and / or (b) a protein which has the same function as (a) and is derived from (a) by substitution, deletion and / or addition of one or more amino acids in SEQ ID NO:2. The recombinant collagen sponge material is obtained by sequential physical cross-linking and chemical cross-linking of the recombinant collagen. The recombinant collagen sponge material according to the present disclosure is capable of hemostasis, wound surface repair, moisture absorption and platelet aggregation, and has high moisture absorption, a significant hemostatic effect and good biocompatibility, assuming great clinical significance in the field of surgery.

The invention relates to a bionic collagen matrix with a density gradient double-layer structure, and belongs to the technical field of medical materials. The collagen matrix takes telopeptide collagen derived from mammalian dermis or achilles tendon as a main component, and comprises a double-layer structure of a compact layer and a loose layer, and the compact layer is smooth in surface and compact in structure, so that cell migration can be blocked, and a certain protection effect is achieved; the loose layer is of a porous structure, cell ingrowth and blood clot stabilization are facilitated, and a support is provided for tissue repair. The preparation method of the biological membrane comprises the following steps: preparing telopeptide-removed collagen gel, preparing collagen sponge, preparing a collagen tissue regeneration membrane, and compounding the collagen tissue regeneration membrane and the collagen sponge into an integrated double-layer structure through bonding, drying and crosslinking. The double-layer structure simultaneously meets the double requirements of barrier protection and tissue regeneration, the process is simple and controllable, and the double-layer structure is suitable for clinical scenes such as oral soft tissue repair, alveolar ridge reconstruction and tooth extraction pit coverage.

The invention discloses collagen sponge and a preparation method and application thereof. The preparation method comprises the steps of pretreatment, acidolysis, enzymolysis, salting-out, filtration, ultrafiltration and concentration, crosslinking, freeze-drying treatment and the like. The preparation process of the collagen sponge provided by the invention is simple to operate, the water absorption capacity of the prepared collagen sponge product can reach 40-60 times of the self weight, the hemostatic effect is good, the mechanical property is good, the requirements of industrial standards are met, and the application range is wide.

This invention discloses a recombinant human collagen cross-linked sponge and its preparation method. The method involves first hydrolyzing a silane coupling agent under alkaline conditions to form a hydrolysate, then adding recombinant human collagen, mixing thoroughly, freeze-drying to form a sponge, and finally heating to cross-link and solidify it, thus obtaining the recombinant human collagen cross-linked sponge. This invention offers a simple preparation method, and the resulting recombinant human collagen cross-linked sponge exhibits excellent mechanical properties, stable biodegradability, and no cytotoxicity, making it a promising candidate for human repair materials.

The application relates to the field of biological medicine, and particularly relates to a cold storage solution and application of a combined traditional Chinese medicine compound biological stent drug delivery system in eardrum repair. The cold storage solution comprises a basic solution and L-alanyl-L-glutamine; the basic solution comprises a STEEN solution. The cold storage solution can promote metabolism and synthesis of glutathione of fat grafts, regulate ROS mitochondrial oxidative stress, reduce cold storage damage of the fat grafts, and improve the cold storage quality of the fat grafts. Meanwhile, the application uses Shengji Yuhong collagen sponge which contains an important classical prescription for promoting repair, forms a functional biological material with supporting and pro-angiogenic effects, forms a linkage mode of "double-stage regulation before and after transplantation", closely combines the actual difficulties of cell damage of fat groups and reduction of repair success rate caused by long eardrum repair time in the clinic, explores a brand-new research mode for improving the eardrum repair scheme, and has far-reaching clinical significance.

The present invention relates to the technical field of medical dressing materials, and more specifically to jellyfish collagen sponges and methods for producing the same. Step S1: Adjust the concentration of a jellyfish collagen raw material with a molecular weight of 80 kDa or more with pure water to obtain a collagen solution with a mass concentration of 0.2 to 0.5%. Step S2: Add ethanol to the jellyfish collagen solution, stir, and let stand. Step S3: Obtain a precipitate by centrifugation or obtain a filter cake by suction filtration. Step S4: Freeze-dry under vacuum to obtain a collagen sponge. 60 The present invention includes step S5, which involves radiation sterilization, packaging, and storage. The collagen sponge produced by this invention has a dense structure, the collagen does not denature, it has strong liquid absorption capacity, and it is resistant to deformation and collapse even when exposed to water. The manufacturing method of this invention avoids technical problems such as the inclusion of impurities due to salting out, low pH precipitation efficiency, or low mechanical strength of the collagen sponge.

The invention relates to a profiled sheet material which has a resorbable porous collagen sponge in which resorbable nanoparticles or microparticles or combinations thereof are dispersed, wherein the nanoparticles or microparticles have at least two different release profiles for the controlled and / or delayed release of pharmaceutical active ingredients. The invention further relates to a method for producing the sheet material and to the use thereof in a method for treating wounds or in a method for local pharmacotherapy.

The invention relates to a method for preparing collagen sponge by using oxidized sodium carboxymethyl cellulose. Comprising the following steps: (1) preparing an oxidized sodium carboxymethyl cellulose solution; (2) adding collagen into acetic acid, and dialyzing for 4-5 days to obtain a collagen solution; and (3) adding the oxidized sodium carboxymethyl cellulose solution into the collagen solution, carrying out cross-linking reaction at 0-4 DEG C for 20-30 hours, and freeze-drying to obtain the collagen sponge. OCMC is adopted as a cross-linking agent for preparing the collagen sponge for the first time, the preparation method of the collagen sponge and the optimal adding amount of oxidized sodium carboxymethyl cellulose are determined, and the prepared collagen sponge is higher in mechanical strength, better in water absorption performance and excellent in hemostatic effect in clinical use. The hemolysis rate of the collagen is far lower than 5%, and the collagen has an obvious blood coagulation promoting effect, does not generate cytotoxicity, does not influence the safety of the collagen as a natural biological material, and can be used for preparing a medical material for hemostasis.

The present application relates to the technical field of medical devices, and particularly relates to a blood vessel puncture plugging device, which comprises: a plugging sheet, which is in a shape of adhering to a blood vessel wall after being unfolded; a suture, one end of which is connected with the plugging sheet, and the other end of which is placed outside the blood vessel wall after passing through a puncture hole; a collagen sponge, which passes through the suture and adheres to the outside of the blood vessel wall; and a lock, which is sleeved on the suture and is used for locking the collagen sponge, so that the plugging sheet tightly adheres to and covers the puncture position of the blood vessel wall; wherein one side of the plugging sheet, which faces the blood vessel wall, is provided with micro-needles in a protruding manner, and the micro-needles are inserted into the blood vessel wall when the plugging sheet adheres to the blood vessel wall. By means of the micro-needles inserted into the inner wall of the blood vessel, the present application provides mechanical fixing force compared with the prior art, prevents the slippage of the plugging sheet, and further improves the reliability of puncture plugging.

To provide a collagen sponge excellent in mechanical strength and a production method for the collagen sponge. A collagen sponge including a porous construct having a pore structure, the collagen sponge having a tensile strength of 1 N or more and 5 N or less in every direction including a length direction and a width direction. The collagen sponge may be produced by a production method including the following steps: (1) a step of subjecting a collagen solution obtained by mixing collagen and a solvent to stirring and deaeration treatment; (2) a step of subjecting the collagen solution to freeze-dry treatment; and (3) a step of subjecting a dried collagen product after the freeze-dry treatment to insoluble treatment.

The utility model provides a protective dressing structure for the face of a patient, which belongs to the technical field of medical instruments and comprises a protective dressing and a mask arranged on the protective dressing, and the protective dressing is freely cut into an integral structure. According to the utility model, after the protective dressing is pasted on the face of a patient, the mask is fixed on the protective dressing by aligning the magic male tape and the magic sub tape, and meanwhile, the whole mask is hung and fixed on the ear through the elastic hanging rope, so that the protective dressing is further fixed, and the protective dressing can be more tightly attached to the skin of the face of the patient; meanwhile, the collagen sponge dressing layer and the alginate fiber dressing layer are arranged, so that a large amount of percolate can be quickly absorbed when the wound exudates; and the original protein sponge can be gradually decomposed and absorbed by enzymes of a human body while infection caused by seepage accumulation is prevented, wound healing is accelerated, and a patient can be helped to recover more quickly.

The invention discloses a bacterial cellulose / collagen composite active hernia patch material and an in-situ biosynthesis method thereof. The method comprises the following steps: extracting collagen from a collagen extraction raw material to obtain purified collagen sponge, dissolving the collagen sponge in water to obtain a collagen solution, adding an oil phase into the collagen solution, and emulsifying to obtain a collagen-based Pickering emulsion; and then, by taking the collagen Pickering emulsion as a template, forming the bacterial cellulose / collagen composite active hernia patch material in an acetobacter xylinum in-situ fermentation culture manner. The bacterial cellulose / collagen composite active hernia patch material with a macroporous structure on one side and an original compact structure on the other side is provided, the macroporous structure of collagen provides a channel for migration of cells and transportation of metabolites, and the compact structure of bacterial cellulose keeps the original high mechanical strength of bacterial cellulose; therefore, the material is favorable for promoting hernia repair.

The application provides a performance enhanced collagen sponge and a preparation method thereof. First, a collagen gel is prepared through an improved dialysis process, then the collagen gel is compounded with glycerol to form a collagen solution, then the solution is treated by using an irradiation cross-linking technology, and finally the product is prepared by irradiation sterilization after vacuum freeze-drying. The product of the application is directly dialyzed without acid dissolution, and the subsequent dialysis is performed by using a low-concentration phosphate buffer solution, so that the pH of the dialysis solution can be quickly adjusted to neutral, and the production cycle of the dialysis process is greatly shortened. In addition, by using the low-concentration phosphate buffer solution dialysis combined with the irradiation cross-linking process, the rehydration of the product is fast under the premise of ensuring the mechanical strength of the product, the formation of a 'dry core' in the material is avoided, the cell growth into the material is facilitated, and a certain amount of glycerol is added in the collagen solution before irradiation cross-linking, so that the collagen can be prevented from shrinking and deforming after irradiation cross-linking, and the size change of the product can be reduced.

The utility model discloses a collagen sponge cutting device, which comprises a blade group and a cutting base. The blade group consists of one or more blades arranged parallel and equidistant from each other and fixing devices located at both ends thereof. The cutting base is in an overall shape of a cuboid or a cube and comprises a bottom tray. Above the bottom tray, two knife rests and two enclosures are symmetrically provided respectively. The knife rests and the enclosures together form a groove for placing the collagen sponge. The knife rest is composed of a number of partitions arranged parallel and equidistant from each other. A partition gap for accommodating the blade is formed between adjacent partitions. The structure of the utility model is simple, the operation is convenient, it is firm and durable, and it can cut the collagen sponge in block specification into ultra-thin sheets with regular shapes and uniform thicknesses. While achieving rapid cutting, it will not compress the collagen sponge and can maintain the fluffy texture of the sponge, so that the collagen sponge is easy to be rapidly hydrated and dissolved to prepare a solution / gel for use.

The present invention relates to the technical field of medical dressings, and in particular to, a jellyfish collagen sponge and a preparation method therefor. The preparation method comprises the following steps: S1. adjusting the concentration of a collagen raw material with a molecular weight of 80 kDa or above with pure water to obtain a collagen solution with a mass concentration of 0.2%-0.5%; S2. adding ethanol into the described collagen solution, stirring, and letting to stand; S3. centrifuging to obtain a precipitate or carrying out suction filtration to obtain a filter cake; S4. freeze drying under vacuum to obtain the collagen sponge; and S5. carrying out irradiation sterilization by using Co60, packaging, and warehousing. The collagen sponge prepared in the present invention features dense structure with collagen undenatured, and it exhibits strong liquid absorption capacity and is not prone to deformation and collapse when in contact with water. According to the method of the present invention, the technical problems including impurity introduction and low pH precipitation efficiency associated with salting-out, or low mechanical strength of the collagen sponge and the like can be avoided.

The invention provides a method for preparing recombinant human collagen sponge. The method comprises the step of carrying out concentration and assembly treatment on recombinant human collagen so as to obtain the recombinant human collagen sponge, and the recombinant human collagen does not have terminal peptide. According to the method disclosed by the embodiment of the invention, the recombinant human collagen sponge which has collagen with a complete triple-helix structure, excellent biological activity, low immunogenicity, high liquid absorption performance, high mechanical strength and no animal-derived virus pollution risk can be prepared by the method.

The present application relates to the medical biomaterial and the wound repair technical field, especially collagen dressing and its preparation method, the preparation method includes the following steps: with suspending agent to scolopendra powder is wrapped, obtains A component;Collagen solution and wetting agent are mixed, and B component is obtained;A component and B component are defoamed, mixed, and C component is obtained;The C component is freeze-dried, and collagen sponge is obtained;The collagen sponge is crosslinked at 90~120 DEG C temperature, and collagen dressing is obtained.The present application is through introducing scolopendra powder to give the function of antibacterial, anti-inflammatory, analgesic, anticorrosive, strong healing of dressing;At the same time, through preparation process to improve the physical properties of dressing, strengthen its dressing structure stability and the hydrophilicity and uniformity of scolopendra component, reduce the clinical use safety risk, make it suitable for all kinds of wound, especially infected wound, deep wound and difficult healing wound, meet the clinical treatment demand.