Method for simultaneously detecting a plurality of mycotoxins in sesame paste

A technology for mycotoxins and sesame paste, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of not being able to detect all mycotoxins, the complex matrix of sesame paste, and the simultaneous detection of sesame paste, etc., to save detection costs and achieve high sensitivity , good repeatability
CN104535664AActive Publication Date: 2015-04-22PEOPLES REPUBLIC OF CHINA BEIJING ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Applications(China)
Current Assignee / Owner
PEOPLES REPUBLIC OF CHINA BEIJING ENTRY EXIT INSPECTION & QUARANTINE BUREAU
Publication Date
2015-04-22

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Abstract

The invention relates to a method for detecting a plurality of mycotoxins in a sesame paste, and particularly relates to a method which carries out pre-treatment by an optimized dispersive solid-phase extraction method (QuEChERS), and simultaneously detects 26 mycotoxins in sesame paste by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS / MS). The method particularly comprises: extracting a sample twice with acid-containing acetonitrile-aqueous solutions with different concentrations, adding magnesium sulfate and sodium chloride into the sample extract for salting out, performing centrifugation, adding n-hexane into the obtained supernatant, performing vortex oscillation and degreasing, purifying the product with C18 and magnesium sulfate, performing vacuum concentration, dissolving the residues again with methanol and water in order to obtain a sample analytic solution, and detecting the solution by a multi-reaction monitoring mode of ultra-high performance liquid chromatography-tandem mass spectrometry. The method improves the detection efficiency, saves detection cost, is high in sensitivity, high in repeatability, and high in adding standard recovery rate, and can be extended to detection of other samples with a high grease content.
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Description

technical field

[0001] The invention relates to a method for detecting various mycotoxins in sesame paste, in particular to a method for pretreatment using dispersive solid-phase extraction (QuEChERS) and simultaneous detection using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS / MS) The method of 26 kinds of mycotoxins in sesame paste belongs to the field of food safety testing. Background technique

[0002] Mycotoxins are toxic metabolites produced by toxin-producing molds under suitable environmental conditions, and have been a huge potential threat to humans, animals and plants since ancient times. In 1960, more than 100,000 turkeys were poisoned and died in the United Kingdom. Studies have found that moldy peanut cake powder imported from Brazil was included in the feed. Feeding rats with this peanut cake powder can induce liver cancer and substances that induce liver cancer It was confirmed to be a metabolite of Aspergillus flavus, nam...

Claims

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