745 results about "Solanum" patented technology

The invention relates to a strain of lys-starch bacillus as well its application. The bacterium is separated from the soil on eggplant fields, anning district, Lanzhou, Gansu. The bacterium is identified to be Bacillus amyloliquefaciens. It's preserved in the Chinese typical culture preserving center (CCTCC) with a preserving name of Bacillus amyloliquefaciens I7 and preserving code of M206027. The broth of M206027 and M206027strain, non-cell culture fluid of M206027 strain's broth, and the monomer extracted from the M206027strain, M206027 broth or its non-cell culture fluid can be used in the biological control of peach anthracnose bacterium, pear nigrities artis bacterium, apple brown rot bacterium, Fusarium acuminatum, Fusarium oxysporum, Fusarium semitectum, chilli black rot bacterium, cucumber blight bacterium, tomato early blight bacterium, eggplant early blight bacterium, dry thread Pyrenomycetes, wheat foot rot bacterium, potato thread Pyrenomycetes, potato early blight bacterium, potato late blight bacterium and watermelon downy mildew bacterium. It's easy to get the material of the invention and easy to operate; besides, it can be widely used in full scale production.

The invention discloses Bacillus subtilis, which is named Bacillus subtilis. The Bacillus subtilis was collected in China General Microbiological Culture Collection Center on January 15th, 2009, with a collection number of CGMCC No.2843. The Bacillus subtilis strain disclosed by the invention is screened from tobacco leaf habitats. Experiments prove that the fermentation liquor of the strain has a strong inhibition effect on fungal pathogens such as Alternaria alternate and pepper anthracnose and demonstrates high prevention effect on Alternaria alternate, tobacco black shank, pepper anthracnose, tomato wilt, phytophthora melonis Katsura, verticillium dahlia and the like and has high growth promoting effects on tobacco, pepper, cucumber, tomato and eggplant. Therefore, the Bacillus subtilis disclosed by the invention can be used for preparing biological bactericides for preventing and controlling fungi in tobacco and vegetables and can be used for preparing growth promoting agents for promoting the growth of tobacco or vegetables.

The invention discloses a plantation method for eggplants. The plantation method for the eggplants is scientific and reasonable. Detailed introduction is made on seed selection, seedling cultivation, field planting, field management and the like, the steps are simple, operation is convenient, popularization is easy, a traditional plantation mode for the eggplants is changed, rapid growth of the eggplants is promoted, the disease-resistant ability of the eggplants is improved, the disease occurrence rate of the eggplants is lowered, and therefore the eggplants can be high in quality and yield, and the economic benefit of vegetable growers can be increased.

The present invention relates to methods and compositions for reducing the appearance of dynamic facial wrinkles by administering oral and/or topical compositions comprising therapeutically-effective amounts of extracts of one or more relaxing herbal agents (“RHAs”) alone or in combination with one or more extracts of an edible solanaceous glycoalkaloid-containing plant (“ESGP”) or an edible glycoalkaloid-containing fungus (“EGF”) selected from plant species in the genus Solanum and mushrooms. RHAs are selected from the group consisting of extracts of Q. amara, E. angustifolia, P. oleracea, M. dubia, P. orientalis and A. muscaria and plant species from the genus Valeriana, Passiflora, Verbascum, Scutellaria, Cypripedium, Magnolia, Atriplex and Symphytum. Additionally, the methods of the present invention may comprise exfoliating the skin, preferably with at least one acid protease enzyme.

The invention belongs to the technical field of crop cultivation and especially relates to a cultivation method for eggplant seedlings. The cultivation method comprises the following steps of selecting the variety of the eggplant; pre-processing the seeds; allocating nutrient soil with the characteristics of evenly mixing 4 parts of rich rural land, 3 parts of decomposed wormcast and 3 parts of sieved fine slag, adding 1kg calcium superphosphate into one cubic meter of bed soil, flatly paving 6-10cm thick nutrient soil on a seedbed, and filling and slightly compacting the rest nutrient soil into a seedling-culturing caved plate for standby; mixing 8-10kg 50% thiram wettable powder or 50% carbendazim wettable powder into 3kg rural land, fully mixing and making into drug soil, and evenly scattering the drug soil onto the seedbed; sowing; and performing seedling management. The seedlings cultivated by adopting the nutrient soil and the cultivation method provided by the invention have strong growth vigor, advanced root system, strong disease resistance and high adaptability after transplanting.

The invention discloses a special seedling medium for solanaceous vegetables, and belongs to the technical field of soilless cultivation. The medium comprises following ingredients in volume ratio according to the formulation: 20-35% of vinegar residue, 15-25% of vermiculite, 20-25% of grass carbon, 15-20% of plant ash and 5-10% of perlite, as well as small amount of inorganic fertilizers such as 0-0.5kg/m<3> of ammonium sulfate nitrate, 0-0.3kg/m<3> of potassium chloride and 0-0.3kg/m<3> of monoammonium phosphate. Organic wastes such as the vinegar residue serve as materials; a soilless medium is produced after high-temperature fermentation treatment; and waste is changed into precious resources, and disadvantages are changed into advantages. The special seedling medium for the solanaceous vegetables produced by the invention has the advantages of high nutrient content, porous permeability, moisture and fertility preservation and the like, and can be widely applied to seeding cultivation of the solanaceous vegetables such as hot peppers, eggplants and tomatoes.

The invention discloses application of solanum lycopersicum juice in lactobacillus (Lb.) plantarum ST-III fermentation process. The invention also discloses probiotic drinks obtained through Lb. plantarum ST-III fermentation. The probiotic drinks are obtained by inoculating Lb. plantarum ST-III into the sterilized solanum lycopersicum juice and carrying out fermentation at 25-40 DEG C. The fermentation proliferation effect of Lb. plantarum ST-III in the culture medium is equivalent to that in the MRS culture medium commonly used in the laboratories, but the cost spent in obtaining the same dose of ST-III viable bacteria through fermentation is reduced by 80% in comparison with the cost when the MRS culture medium is used. Thus, Lb. plantarum ST-III can be widely applied to study of Lb. plantarum ST-III and development and industrial production of viable bacteria preparations of Lb. plantarum ST-III. Meanwhile, the Lb. plantarum ST-III fermentation liquor with a high viable count, cultured by taking the non-additive pure solanum lycopersicum juice as the fermentation base material, can be directly drunk as the lactobacillus drink having a health care function.

The invention provides an eggplant seed germination accelerating method comprising seed selection, seed soaking with a seed soaking agent, microwave nutrition liquid seed soaking, refrigerator temperature-varying seed storage, rare earth seed dressing, and finally germination accelerating. The germination accelerating method can improve the seed germination rate, shortens the germination accelerating time, and relieves the field incidence rate; and moreover, eggplant planted after germination accelerating is flourishing in leaves of a growing period, and strong in drought resistance and cold resistance, and eggplant fruits after maturing are large in size and good in taste.

The invention discloses a lilium regale WRKY transcription factor gene LrWRKY4. The nucleotide sequence of the lilium regale WRKY transcription factor gene LrWRKY4 is described as in the SEQ ID NO:1,and the coded protein of the lilium regale WRKY transcription factor gene LrWRKY4 corresponds to the amino acid sequence described in the SEQ ID NO:2. According to the lilium regale WRKY transcriptionfactor gene LrWRKY4, it is proved that the LrWRKY4 gene has the function of improving the antifungal ability of plants through the technical research related to the functional genomics, the antifungal LrWRKY4 gene is constructed to a plant expression vector and transferred into tobacco for overexpression, the transgenic tobacco has very high antifungal activity, and the experimental result showsthat the tobacco with the LrWRKY4 gene overexpressed is highly resistant to infestation of nigrospora oryzae, fusarium graminearum, fusarium graminearum, botryosphaeria and fusarium solanum.

The invention discloses a solid high-efficiency cultivation method for inter-planting cane and eggplants, changes the traditional the cane-row with sharp ridge and equal distance into wide and narrow rows with flat furrow and perforates and inter-plants the double-row eggplants in wide rows; the invention arranges the planting time of the eggplants and the canes reasonably, solves the mutual influence of symbiotic period of main crops and subsidiary crops, increases planting density, improves the inter-planting efficiency, adopts a degrading mulching film to cover, improves the yield and the quality of intercrop crops by fully using the condition of light, temperature, water and fertilizers, advances the planting time and the picking time, reduces the cost of work for removing the mulching films, adopts two-time single-side fertilizing and moulding method, prolongs the harvest time of the eggplants and ensures the normal growth of the canes; the average yield of the eggplant is 16 ton per hectare, the average yield of the canes is 125 ton per hectare and is increased by 12 ton per hectare compared with the contrast average yield of the canes. Comprehensive economic benefit is increased compared with the contrast economic benefit.

The invention discloses a method for inducing eggplant anther to regenerate a haplobiont. The method mainly comprises the following steps: (1) selecting anther in a proper period and conducting low-temperature pretreatment on the anther; (2) sterilizing the surface of an explant and inoculating; (3) conducting high-temperature dark treatment; (4) conducting light radiation culture and inducing callus to be generated; (5) conducting callus culture proliferation; (6) callus differentiating adventitious buds; (7) grafting bud seedlings, breeding plants in an expanding mode and subculturing; (8) enabling small rootless seedlings to grow roots and become complete plants. The method is high in possibility of obtaining callus, high in callus differentiating frequency, and good in culturing effect. When the method is used in eggplant genetic breeding, the culturing efficiency of eggplant anther can be greatly improved, good eggplant genotype individuals can be enriched, an eggplant haploid breeding method is perfected, a breeding cycle is shortened, and technologies and theoretical basis are supplied for study of genetic breeding and anther in vitro culture of eggplants and other solanaceae crops.

The invention relates to an efficient cultivation method for interplanting pinellia ternate and vegetables, comprising the steps of: interplanting pinellia ternate under vegetables, such as hot pepper, eggplants, and the like; covering the pinellia ternate with corrosive manure grass; carrying out the management of working procedures, such as building tunnel, fertilizing, removing grass, desinsection, covering bulbel by the manure grass, and the like; building small tunnels for heat preservation to prolong growth period after temperature drop; and directly turning the manure grass and harvesting after final seed falling, and remaining seeds. The production method utilizes the same land to produce two kinds of crops simultaneously, and increases multiple planting index; the manure grass is adopted for covering so that cultivation can be removed, and the growth of weed and the occurrence of underground disease can be reduced; ventilation and water retention can be performed, and fertility is improved; the growth of rhizome expands and accelerates, the output is improved, and the working period is reduced during harvest; and the labor cost is saved, the production cost is reduced, the output is improved, and the economic benefit is multiplied.

The invention pertains to a method to grow mushroom, providing a black fungus growing method by replacing sawdust bags with crop straws collected from vegetable gardens. The culture medium takes green-bean seedling, eggplant seedling and pumpkin seedling as main materials; a small amount of bran, cane sugar, gypsum powder and calcium superphosphate are added; the growing method includes the steps of material mixing, water adding, mixing, material moistening, bagging, sterilization, cooling, inoculation, fungus cultivation and sprouting, opening and management before and after germination. The material batching method and the growing method ensure rapid sprouting of the black fungus, free of bacterium contamination, uniform sprouting and high yield. The yield is 1-3 times of the yield achieved by the traditional growing method based on sawdust, and the cost is low.

The invention discloses a gene sequence for synthesizing a relative transcription factor SmMYB by eggplant anthocyanidin, comprising a nucleotide sequence shown in 1st-1035th nucleotides in SEQ ID NO.1, wherein a polypeptide coded by the gene sequence comprises an amino acid sequence is shown in SEQ ID NO.2. The gene sequence in the invention is a key regulation and control gene for synthesizing eggplant anthocyanidin, the relative transcription factor SmMYB is synthesized by eggplant anthocyanidin. A downstream gene which is regulated and controlled by the gene sequence and is closely related to anthocyanidin synthesis, and other transcription factor genes manually acting with the gene sequence, are screened. The gene sequence in the invention is applied to obtain an eggplant kind with high anthocyanidin content and excellent resistance quality.

The invention is a method of inducement of isolated microspore callus of eggplant and plant regeneration. The major procedures are as follows. Appropriate anther is selected for pre-cultivation. Then dissociation and collection of microspore are carried out. And then static shallow pan dark culture is carried out in fluid nutrient medium. After 20 to 30 days, callus emerges successively. Callus is taken for cultivation under sunlight after emergence. When the callus grows to the size with the diameter of 2 to 6 millimeters, callus is transferred to solid regeneration culture medium for further cultivation. After 30 to 45 days, callus begins to differentiate to form buds. When bud grows to size of 1 to 3 centimeters, callus is transferred to rooting culture medium. After 10 to 15 days, strong root is developed. By adopting the method, the probability of obtaining haploid is high and frequency of microspore callus genesis is high. The invention is not only the technological breakthrough of eggplant haploid breeding, the inducing system of isolated microspore for cultivation of embryoid can also be a good experimental system for carrying out research on plant cell differentiation and development, molecular marker and gene map.

The invention discloses a step-shaped circular pruning method of eggplant. After the plant is planted, a side bud is reserved below the first flower of the main stem, and together with the main stem, two trunks are formed and distributed in a "Y" shape. The two trunks Cultivate fruiting mother branches, and each fruiting mother branch cultivates 5 to 7 fruiting short branches in turn. When each fruiting short branch sits on a fruit, keep 2 leaves for topping after the fruit, and the eggplant on each fruiting short branch is picked. Finally, cut off the fruiting short branch at a distance of 0.8~1.2 cm from the fruiting mother branch. The eggplant is pruned by the step-shaped circular pruning method, which greatly increases the yield. The step-shaped circular pruning method can control the plant height and solve the problem of multiple drop seedlings. The problem of reducing labor costs can improve the ventilation and light transmission conditions in the field, and help reduce the occurrence of pests and diseases.

The invention discloses an alpine grassland pasture endogenous serratia plymuthica strain GH010 capable of inhibiting various pathogenic bacteria and discloses a microbial agent prepared from the alpine grassland pasture endogenous serratia plymuthica strain GH010 and a preparation method thereof. The alpine grassland pasture endogenous serratia plymuthica strain GH010 and the microbial agent prepared from the same disclosed by the invention have the beneficial effects of having good inhibition effects on the various pathogenic bacteria; the inhibition effect is 67.5% of chili rhizoctonia solani and 69.5% of rape sclerotinia sclerotiorum; the bacteriostasis rates of cotton rhizoctonia solani, eggplant sclerotinia sclerotiorum, tomato botrytis cinerea, fusarium wilt of cucumber, exserohilum turcicum, bipolaris maydis or bipolaristriticola are more than or equal to 57.5%; and the strain has growth-promoting effects on crops including alfalfa and the like, and the environmental protection properties of a microbial pesticide and a microbial fertilizer prepared from the strain are good, the resistance to drugs is unlikely to generate and the safety is good.

The invention provides nutritional soil for cultivating eggplant seedlings and a preparation method of the nutritional soil. The preparation method comprises the following steps: firstly, selecting fermented cow dung, fermented rice husk chicken manure and composted pleurotus ostreatus residues and other raw materials which are commonly used in daily production according to growth demands of the eggplant seedling stage; secondly, preparing special nutritional soil for cultivating the eggplant seedlings by multiple formulation screening experiments. The nutritional soil is economical and practical as well as is beneficial to growth of the eggplant seedlings; in addition, during the seedling cultivation period for two months or longer, spraying of fertilizer is not needed, and the eggplant seedlings vigorously grow; the nutritional soil is a better choice for cultivating the eggplant seedlings. At present, generally-applied cultivation matrixes in the world are peat and rock wool, but the two matrixes are expensive, peat resources are unrenewable, and the rock wool is non-degradable, therefore, all countries in the world are trying to study substitutes of the peat and the rock wool. At the same time, the fermented cow dung, the fermented rice husk chicken manure, the composted pleurotus ostreatus residues and the like are used, so livestock manure and agricultural wastes are enabled to be recycled, and replace the non-renewable peat, and further resource conservation and environment friendliness are facilitated.

The invention discloses a gene related to plant parthenocarpy and particularly relates to an auxin response factor SmARF8 for regulating and controlling the parthenocarpy of an eggplant, wherein the full length of a cDNA sequence of the gene is 3,750bp. The invention also relates to a method for interference expression of the gene related to the parthenocarpy in the eggplant. The gene can ensure that a transgenic plant produces the parthenocarpy, thereby improving the quality, prolonging the preservation storage period, and having a simple operation method.

The invention discloses a CAPS (cleaved amplified polymorphic sequence) molecular marking method for identifying solanum lycopersicum with purple black striped pericarp and an application. The sequence of a molecular marker carried by the solanum lycopersicum with purple black striped pericarp is a nucleotide base sequence shown as SEQ ID NO.1, PCR amplification is performed with genomic DNA of the solanum lycopersicum with purple black striped pericarp as a template, nucleotide base sequences shown as SEQ ID NO.3-4 are amplification primers, amplification and cleavage are performed, a specific band different from other solanum lycopersicum pericarp colors can be obtained after cleavage, and the molecular marking method is applied to breed improvement and breeding of the solanum lycopersicum. Compared with the prior art, the CAPS molecular marking method has the advantages that the polymorphism of a cleaved fragment is observed through PCR amplification, cleavage and gel electrophoresis separation, the step of membrane transfer in the traditional RFLP (restricted fragment length polymorphism) analysis is omitted, the operation is simplified, the precision of the RFLP technology is kept, the polymorphism detecting probability is higher, the solanum lycopersicum with purple black striped pericarp is obtained rapidly and applied to breed improvement and assisted breeding, and the method has important theoretical and practical meanings for culturing new varieties of solanum lycopersicum containing rich anthocyanidin.

The invention relates to a method for rapidly identifying salt tolerance of eggplants and belongs to the technical field of plant cultivation and breeding. The method comprises carrying out salt stress treatment on 15 varieties of eggplants in the four-leaf and one-hearted eggplant seedling stage for 10 days, then detecting leaf proline content, a chlorophyll loss rate and a salt damage index after treatment for 10 days, calculating single membership function values of the leaf proline content, chlorophyll loss rate and salt damage index of 15 varieties of eggplants, calculating a comprehensive membership function value of each variety of the eggplant, carrying out comprehensive evaluation on the salt tolerance and building an evaluation system. The salt-tolerance membership function valuecan evaluate the salt tolerance of the plant through a comprehensive index and the evaluation result is close to the actual one. Through the eggplant seedling comprehensive membership function valueafter salt stress, the salt tolerance of the eggplant can be simply, quickly and accurately identified.

A method which utilizes grafting of wild aubergines to prevent verticillium wilt of aubergines is applied to producing aubergines in protected grounds or outdoors in our country. The invention provides the method which utilizes wild aubergines with the name of super-support and cultivating aubergines grafting. The invention adopts the proposals that firstly, parental stocks and grafts are selected, which adopt wild aubergines with the name of super-support as parental stocks, the seeds of the wild aubergines are small and yellow-white, and the thousand seed weight is 1g, the natural growing height reaches 2m to 2.5m, the thicknesses of stems reach 10cm to 20cm, the diameters of fruits are 1-2cm, the color of peels is white-green in patterns. Grafting assortments are green aubergines, secondly, the grafting method is that parental stocks wild aubergines with the name of super-support are firstly sowed, during the period when two sheets of cotyledons are flattened, grafts are further sowed, when wild aubergines grow 5-7 sheets of true leaves, the wild aubergines are grafted by adopting the cleft grafting method, 2-3 sheets of true leaves are reserved beneath the parental stocks, 2-3 sheets of true leaves are reserved above grafting seedlings, the depth of notches is about 1.0-1.5cm, the healing temperature is 25-26 degrees in the daytime and 20-22 degrees in the night, and the humidity of the air is more than 95%.