616 results about "Cold resistance" patented technology

The invention discloses a high-yield cold-resistant pennisetum germplasm screening method and application thereof, and the method is based on 10 yield traits, 4 cold-resistant indexes and 4 low-temperature photosynthetic ability indexes of pennisetum, constructs a multi-dimensional dynamic monitoring system across autumn, winter and spring, and performs high-yield cold-resistant comprehensive evaluation on the pennisetum germplasm, thereby screening out a high-yield cold-resistant breeding material. The invention provides a comprehensive, efficient and accurate high-yield cold-resistant Chinese pennisetum germplasm screening method, through a multi-dimensional dynamic monitoring system crossing autumn, winter and spring, yield traits, cold resistance indexes and low-temperature photosynthetic ability indexes are integrated, and the problems that a traditional method is single in evaluation dimension, disjointed with field reality and insufficient in dynamic adaptability are solved. The comprehensive evaluation system based on the principal component quantitative model not only fills the blank of the high-yield and cold-resistant evaluation model of the pennisetum alopecuroides, but also remarkably improves the breeding efficiency, provides powerful support for cultivating cold-resistant and yield-reduction-free breakthrough varieties, and has important application value and popularization prospect.

The invention discloses a low-temperature-resistant PVC / SEBS modified material and a preparation method thereof, and belongs to the technical field of high polymer materials. The PVC / SEBS modified material comprises the following raw materials in parts by weight: 80-100 parts of PVC, 20-40 parts of SEBS, 5-20 parts of a plasticizer, 6-10 parts of a cold-resistant agent, 2-4 parts of a flame retardant, 5-8 parts of an anti-aging agent, 2-3 parts of a stabilizer and 3-5 parts of a lubricant. The PVC / SEBS modified material prepared by the invention has excellent cold resistance.

The invention discloses a PgEGY3 gene for improving cold resistance of pennisetum alopecuroides and increasing fresh weight of leaves and application of the PgEGY3 gene, and relates to the technical field of genetic engineering, and the nucleotide sequence of the PgEGY3 gene of the pennisetum alopecuroides is shown as SEQ ID NO.1. The invention also discloses a protein coded by the Pennisetum alopecuroides PgEGY3 gene. The invention also discloses a recombinant expression vector and a recombinant host cell comprising the Pennisetum alopecuroides PgEGY3 gene. The invention also discloses application of the PgEGY3 gene, a recombinant expression vector and a recombinant host cell in improving cold resistance and biomass of pennisetum alopecuroides. It is verified that the Pennisetum alopecuroides PgEGY3 gene can improve the cold resistance of plants, and the growth state of the plants under cold stress is remarkably improved through overexpression of the PgEGY3 gene.

The invention discloses application of an NtTCP7 gene in cold resistance of tobacco, and belongs to the technical field of molecular biology. According to the application of the NtTCP7 gene disclosed by the invention in the cold resistance of the tobacco, the sequence of the NtTCP7 gene is as shown in SEQ ID NO. 1. The NtTCP7 gene negatively regulates and controls the cold resistance of the tobacco, and the cold resistance of the tobacco is remarkably improved by knocking out the gene. Excavation and functional identification of the gene provide important gene resources and theoretical basis for low-temperature-resistant breeding of tobacco.

The invention provides an aluminum alloy material, a preparation method thereof and a cable. The aluminum alloy material is prepared from the following components in percentage by mass: 0.03 percent to 0.06 percent of Si, 0.80 percent to 1.2 percent of Fe, 0.15 percent to 0.25 percent of Cu, 0.005 percent to 0.03 percent of B, 0.001 percent to 0.02 percent of Ti, 0.05 percent to 0.15 percent of Y, 0.08 percent to 0.2 percent of the total mass of La and Ce and the balance of Al. The specific content of Fe is added into the aluminum alloy material and cooperates with the rare earth elements Y, La and Ce, and the elongation at break of the aluminum alloy material is improved; meanwhile, due to the addition amount of the Y element, the fatigue resistance of the material is further improved, the toughness of the material is improved, and the heat resistance and cold resistance of the material can be improved. A specific content of boron element is added to form nuclear particles, so that the effect of refining grains is achieved, and the tensile strength of the aluminum alloy material is improved; and meanwhile, the resistivity of the aluminum alloy material is further reduced through cooperation of the boron element and other components. And the titanium element can also play a role in refining grains, so that the tensile strength of the material is further improved. The cable prepared from the aluminum alloy material can meet the requirement of a cable torsion experiment.

The invention discloses a sheath material for a saline-alkaline-resistant dragging cable, a preparation method of the sheath material, the cable and the sheath material. The sheath material is prepared from the following raw materials in parts by weight: 40 to 45 parts of chlorinated polyethylene, 1.5 to 2.0 parts of dicumyl peroxide vulcanizing agent, 2 to 2.5 parts of triisocyanate vulcanizing agent, 0.1 to 0.3 part of ethylene thiourea accelerant, 0.05 to 0.15 part of anti-aging agent RD, 4 to 5 parts of metal oxide, 1 to 1.5 parts of paraffin, 8 to 10 parts of calcium carbonate, 15 to 21 parts of reinforcing agent, 1 to 1.5 parts of lead stearate, 0.5 to 1 part of lead sulfate tribasic, 1 to 1.5 parts of ultraviolet light absorber, 3 to 5 parts of epoxidized soybean oil and 8 to 8 parts of chlorinated paraffin. 9 parts of antimony trioxide and 3-4 parts of antimony trioxide. The cable has the advantages of ultraviolet resistance, no salt deposition, water resistance, tensile resistance, cold resistance and the like, can be used for a long time in a special operating environment, has the service life of at least more than 3 years, and avoids frequent replacement of the cable by a user.

The invention discloses a potato cold resistance identification method and system, and relates to the technical field of cold resistance identification. The method comprises the following steps: cultivating potato tissue culture seedlings in a standardized manner, and randomly dividing the seedlings into a low-temperature stress group and a normal-temperature control group; performing standardized low-temperature treatment including slow descent and constant-temperature stages on the stress group; the method comprises the following steps: acquiring multi-source data such as chlorophyll fluorescence parameters, canopy thermal imaging, digital image phenotypes and biochemical indexes, and forming a unified data set after derivative index calculation and standardized fusion; inputting the fusion data into an improved neural network model, training the model through a particle swarm optimization algorithm, and outputting a quantitative potato cold resistance index; automatically evaluating the cold resistance grade according to the potato cold resistance index and generating an identification report; standardization of the identification process, fusion analysis of multi-dimensional indexes and intelligent result analysis are achieved, and the accuracy, efficiency and practicability of potato cold resistance identification are remarkably improved.

The invention relates to the field of automobile part manufacturing, and particularly discloses a barrier layer direct bonding type TPE material for an automobile air conditioner pipeline system and a preparation method and application of the barrier layer direct bonding type TPE material. The TPE material is prepared from the following raw materials: a thermoplastic elastomer TPE, PA, EVOH, polyether block amide, a glycidyl methacrylate grafted olefin copolymer elastomer, a toughening compatilizer, composite modified montmorillonite, a cold-resistant additive, an antioxidant and paraffin oil. Wherein the composite silane coupling agent, lauric acid and N, N '-carbonyldiimidazole are adopted to modify the nano-montmorillonite, so that the dispersity of nano-montmorillonite particles is improved, the formation of aggregates is effectively reduced, and the mechanical property of the material is enhanced; the polyether block amide, the glycidyl methacrylate grafted olefin copolymer elastomer and the toughening compatilizer have a synergistic effect, so that good adhesiveness of the TPE material and the barrier layer is realized.

The invention discloses a cold response PaIBH1 protein and application of a coding gene thereof in enhancing cold stress resistance of plants. The cold response PaIBH1 protein is a protein PaIBH1 with an amino acid sequence as shown in SEQ ID NO: 2. Experiments prove that the gene for coding the protein PaIBH1, namely the gene PaIBH1, is overexpressed in wild type arabidopsis thaliana, so that the cold resistance of the arabidopsis thaliana can be improved; the improvement of the cold resistance shows that after cold treatment, the fresh weight of the overground part is increased, the wilting rate is reduced, the leaf membrane damage and electrolyte leakage are reduced, the photosynthetic ability is improved, the membrane lipid peroxidation damage is relieved, the active oxygen scavenging ability is enhanced and / or the active oxygen accumulation in the plant body is reduced. Therefore, the protein PaIBH1 can improve the cold resistance of the plants. The method has a very wide application prospect in the aspect of cold-resistant genetic improvement of crops or pasture.

The invention discloses an HPVC power cable protection pipe and a preparation method thereof, and relates to the technical field of cable protection pipes. The HPVC power cable protection tube is at least prepared from the following raw materials in parts by weight: 100 parts of high-strength polyvinyl chloride, 35 to 45 parts of epoxy acrylate rubber, 5 to 8 parts of vinyl chloride-g-acrylate copolymer, 8 to 12 parts of epoxidized soybean oil, 3 to 5 parts of calcium-zinc composite stabilizer, 5 to 15 parts of modified hydrotalcite, 0.1 to 0.5 part of beta-diketone, 0.8 to 1.5 parts of calcium stearate and 10 to 20 parts of flame retardant. 12 to 15 parts of modified nano calcium carbonate, 5 to 8 parts of talcum powder, 0.6 to 1.2 parts of antioxidant, 1 to 2 parts of anti-aging agent 4010 and 0.5 to 1.5 parts of ultraviolet light absorber UV531; the modified hydrotalcite is obtained by adding a mixed solution of calcium nitrate and lanthanum nitrate, a sodium hydroxide solution and a sodium bicarbonate solution into a lauryl sodium sulfate solution, mixing and aging, and then carrying out a post-treatment process. The HPVC power cable protection tube prepared by the invention has relatively high strength, good mechanical properties, weather resistance and cold resistance, and long service life.

The invention belongs to the technical field of plant molecular biology, and relates to application of VDAC1 / 3a allele in regulation of tomato cold resistance. According to the invention, the expression quantity of the SlVDAC1 / 3a gene in the VDAC1 / 3a allele is reduced by means of knockout or mutation and the like, so that the cold resistance of the tomato can be enhanced, the yield of the tomato and the content of soluble solids are not negatively influenced, and a theoretical basis and gene resources are provided for cultivating and screening cold-resistant tomato varieties.

The invention belongs to the technical field of agricultural remote sensing, and discloses a method for evaluating cold resistance of pennisetum alopecuroides based on unmanned aerial vehicle multispectral images, which comprises the following steps: acquiring agronomic characters related to cold resistance, such as chlorophyll content, overwintering rate and the like of the pennisetum alopecuroides before and after low-temperature stress through manual measurement, and acquiring the multispectral images by using an unmanned aerial vehicle; the image is preprocessed, and various vegetation indexes such as DVI, NDVI and RVI are extracted; on the basis of agronomic character data, a comprehensive cold resistance index CRI is constructed by adopting principal component analysis and a membership function method; using the vegetation index as an independent variable and CRI as a dependent variable, and using a machine learning algorithm to construct a prediction model; and finally, realizing rapid and nondestructive evaluation on the cold resistance of a large-range pennisetum alopecuroides group by utilizing the model, and outputting a five-level cold resistance grading result according to a CRI value. According to the method, the problems of low efficiency and high difficulty of traditional manual evaluation are solved, high-throughput and precise cold resistance identification is realized, and reliable technical support is provided for cold-resistant breeding and cultivation management of pennisetum alopecuroides.

The invention discloses a KASP marker for auxiliary screening of cold-resistant wheat germplasm and application of the KASP marker, and relates to the technical field of biology, in particular to a method for screening or auxiliary screening of wheat cold-resistant germplasm, which comprises the following steps: detecting whether the genotype of wheat to be detected is genotype qFR-7A. 1a or genotype qFR-7A. 1b; the cold resistance of the wheat with the genotype qFR-7A. 1a is higher than that of the wheat with the genotype qFR-7A. 1b, or the cold resistance of the wheat with the genotype qFR-7A. 1a is higher than that of the wheat with the genotype The wheat of which the genotype is qFR-7A. 1a is wheat of which the genotype is GG homozygous based on a G101A SNP (Single Nucleotide Polymorphism) site; the wheat of which the genotype is qFR-7A. 1b is wheat of which the genotype is AA homozygous based on a G101A SNP (Single Nucleotide Polymorphism) site; the G101A SNP site is the 101 nucleotide from the 5'terminal of SEQ ID NO: 1 in a wheat genome. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of wheat germplasm or breeding offspring materials with relatively high cold resistance.

The invention relates to the technical field of photoelectric composite cables, in particular to a cold-resistant tensile photoelectric composite cable and a rapid laying method thereof, and the cable comprises a conductor part, an insulating layer, an optical fiber unit, a filling layer and an outer sheath which are coaxially arranged from inside to outside and are sequentially wrapped. The conductor part is formed by twisting a plurality of strands of copper wires, a carbon fiber reinforced core is arranged in the conductor part, and the insulating layer is made of EAA and PEBA blending modified materials and contains nano boron nitride particles; the optical fiber unit is of a PBT loose tube structure, a plurality of single-mode optical fibers and special ointment are arranged in the PBT loose tube structure, micro-nano protrusions are arranged on the inner wall of a loose tube, and the surface of each micro-nano protrusion is covered with a silicon dioxide antireflection film. The cable integrates the functions of cold resistance, stretch resistance, electric conduction, insulation, communication, heat dissipation and protection. The insulating layer adopts EAA and PEBA blending modified materials to be combined with nano boron nitride particles, so that the cold resistance and the insulativity are improved; and the polyurethane elastomer of the filling layer is compounded with the glass beads, so that the structural stability and the comprehensive performance are enhanced.

The invention belongs to the technical field of microbial resource development and straw biological decomposition. The invention discloses cold-resistant pseudoarthrobacter psychrotolans CKON-4, and the preservation number of the cold-resistant pseudoarthrobacter psychrotolans CKON-4 is CGMCC (China General Microbiological Culture Collection Center) No.29378. The cold-resistant pseudoarthrobacter psychrotolans CKON-4 has the advantages that the preservation number The strain CKON-4 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on December 25, 2023, the China General Microbiological Culture Collection Center is called CGMCC for short, the address of the China General Microbiological Culture Collection Center is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number of the strain is CGMCC No.29378. The strain disclosed by the invention has very high lignocellulose degradation activity, and the decomposition rate of straws can be remarkably improved; the strain is separated from rice field soil, can grow in the pH value range of 5-9 and the temperature range of 4-37 DEG C, can tolerate the salt concentration of 10%, and is a bacterium with wide pH application range, salt resistance and cold resistance. The strain can play an important role in lignocellulose degrading enzyme production, straw biological decomposition and compost fermentation.

The invention belongs to the technical field of sealing materials, and particularly relates to a rubber composite sealing material and a liquid phase preparation method and application thereof. According to the liquid-phase preparation method of the rubber composite sealing material, provided by the invention, based on deep understanding of a liquid-phase synthesis process of first liquid-phase pre-dispersion and then condensation, a modified carbon black dispersion liquid, a modification auxiliary agent and a modified synthetic rubber latex dispersion liquid are prepared, so that carbon black and the auxiliary agent can be promoted to be uniformly and stably dispersed in a synthetic rubber system; the dispersion uniformity of the carbon black and the auxiliary agent is improved, so that the vulcanized rubber composite sealing material is not easy to have insufficient or excessive local crosslinking or structural defects, thereby improving the mechanical property, air tightness and cold resistance of the rubber composite sealing material, and being a high-performance rubber composite material; the technical problem that a high-performance rubber material is difficult to prepare by an existing preparation method is solved.

The invention relates to a Paenibacillus polymyxa strain B01 and application of the Paenibacillus polymyxa strain B01, and belongs to the technical field of microorganisms. The paenibacillus polymyxa B01 disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number of the paenibacillus polymyxa B01 is GDMCC NO.66594. The strain is obtained by separation, purification and screening from a soil sample, and tests show that the strain has excellent pH and high temperature tolerance; antibacterial active substances generated by fermentation of the bacillus subtilis have a relatively good antibacterial effect on escherichia coli, pseudomonas aeruginosa, pseudomonas putida, staphylococcus aureus, candida albicans, aspergillus brazilis, propionibacterium acnes and malassezia; and the antibacterial active substance has excellent heat resistance, cold resistance, illumination stability, pH stability and preservative effect, belongs to a mild low-irritation raw material, can be used for preparing daily chemical products and medicines, and has wide application prospects and important conversion research values.

The utility model belongs to the technical field of cables, and particularly relates to a crosslinked polyethylene nano-composite power cable, which comprises a cable core, an insulating layer is arranged on the outer side of the cable core, and a buffer layer is filled between the insulating layer and the cable core; a flame-retardant layer is arranged on the outer side of the shielding layer, and a heat preservation layer is arranged on the outer side of the flame-retardant layer. By arranging the insulating layer, the shielding layer, the flame-retardant layer, the heat preservation layer, the anti-freezing layer and the wear-resistant layer, protection on the cable core can be effectively enhanced, and the cable has excellent cold resistance, good insulating performance, good flame retardance, improved wear resistance and pressure resistance when in use; therefore, the service life of the cable is prolonged.

A method for improving degradation efficiency of corn straw at low temperature comprises the steps that cell-free culture fluid PfCFs prepared from Pseudomonas fragi D12 participates in fermentation and degradation of the corn straw, the classification name of the strain D12 is Pseudomonas fragi D12, the strain D12 is preserved in China Center for Type Culture Collection, the preservation address is No. 299, 8th road, Wuchang District, Wuhan City, Hubei Province, China, and the preservation number is CGMCC NO. The preservation number is CCTCC (China Center For Type Culture Collection) NO: M2025126, and the preservation date is January 14, 2025. The acellular culture solution of the pseudomonas rubrassicae D12 can promote the growth of various normal-temperature bacteria and fungi functional bacteria at low temperature and improve the cold resistance of the functional bacteria, and CFS of the pseudomonas rubrassicae D12 also contains related enzymes capable of degrading lignin; at a low temperature, the PfCFs synchronously promotes the co-growth of bacteria bacillus subtilis and fungi Irpex lacteus, so that the degradation efficiency of the functional bacteria on lignocellulose in the corn straws is improved.

The present invention relates to the technical field of molecular markers, in particular to a molecular marker associated with cold resistance of corn and the use thereof. The molecular marker associated with the cold resistance of corn is ZmTIP4;3, and is obtained by amplification using primers shown as SEQ ID NO. 1 and SEQ ID NO. 2. Since the cold resistance of corn is a quantitative trait, phenotype analysis is time-consuming and labor-consuming. The molecular marker can be applied to cold-resistant corn breeding, and identification can be achieved in the corn seed stage or the early stage of cotyledon emergence, which saves time and is accurate, thus accelerating the process of breeding cold-resistant corn varieties.

The utility model discloses a waterproof aluminum alloy conductor flexible cable, which comprises aluminum alloy inner conductors, insulating layers and a wrapping sleeve, a plurality of aluminum alloy inner conductors are arranged in the wrapping sleeve, the insulating layers are arranged on the outer sides of the aluminum alloy inner conductors, and shielding layers are arranged on the outer sides of the insulating layers; the outer side of the wrapping sleeve is coated with a water-blocking layer, and the outer side of the wrapping sleeve is uniformly wound with an expansion water-blocking tape. According to the utility model, the outer sheath sleeves the outer side of the expansion water-blocking tape, the outer sheath can be utilized to carry out preliminary protection on the aluminum alloy conductor flexible cable, the outer sheath is made of rubber, can generate certain deformation and has a certain compression resistance effect, and then the polyurethane anti-freezing sleeve is arranged on the outer side of the outer sheath in an environment-friendly manner, so that the service life of the aluminum alloy conductor flexible cable is prolonged. The oil resistance, the wear resistance and the cold resistance are excellent, temperature protection can be provided for the aluminum alloy flexible cable, and the anti-freezing effect is good.

The invention relates to the technical field of plant genetic engineering, in particular to application of a rice OsERF141 protein and a coding gene thereof in improving low-temperature tolerance of plants. Experiments show that the low-temperature resistance of an OsERF141 function deletion mutant strain is remarkably reduced, and the low-temperature resistance of an overexpressed OsERF141 transgenic strain is remarkably improved. The invention provides a new gene resource for the cold resistance problem of rice, and has important significance for the molecular mechanism research of low-temperature stress and the cultivation of new low-temperature-resistant varieties.

The invention provides a molecular marker composition related to rice seedling stage cold resistance, a primer composition, a kit and application thereof, and belongs to the technical field of crop genetic breeding. The invention provides a molecular marker composition related to rice seedling stage cold tolerance. The molecular marker composition comprises at least one of a first SNP marker, a second SNP marker, a third SNP marker, a fourth SNP marker, a fifth SNP marker and an InDel marker. The invention further provides a primer composition for detecting the cold resistance of the rice in the seedling stage. The primer composition comprises at least one of a first primer group, a second primer group, a third primer group, a fourth primer group, a fifth primer group and an InDel detection primer group. The molecular marker composition and the primer composition disclosed by the invention can be used for accurately distinguishing a cold-tolerant rice variety and a cold-sensitive rice variety in a seedling stage, and scientific guidance is provided for breeding and improvement of a new cold-tolerant rice variety.

The invention discloses a salinity regulation and control seedling culture method for hibiscus syriacus, and belongs to the technical field of hibiscus syriacus seedling culture. The method comprises the following steps: (1) seed treatment; (2) preparing a substrate; (3) after sowing, culturing in a greenhouse; (4) transplanting and salinity control: a) when the seedlings grow to have 3-4 true leaves, transplanting the seedlings into a nutrition bag, adding 0.4-0.6 wt% of a microbial liquid into the matrix at the same time, the microbial liquid containing nitrogen-fixing bacteria Azobacter chrocococcus and phosphate-solubilizing bacteria Bacillus licheniformis, and the total number of viable bacteria being greater than or equal to 1 * 10 < 9 > CFU / mL; after transplanting, carrying out drip irrigation with brackish seawater, and spraying a 0.1% monopotassium phosphate solution on leaf surfaces every half a month for three times in total; b) directly carrying out drip irrigation on brackish seawater in the nutrition bag; and (5) outdoor cold-resistant treatment: 30 days before winter, spraying 0.2-0.5 L of 0.2-0.4 wt% potassium sulfate aqueous solution to leaf surfaces for each plant, spraying once every 6-8 days, and spraying 3-5 times in total. Through salinity gradient domestication and use of the microbial agent, the biomass loss rate of the hibiscus syriacus under the salinity of 0.5% is remarkably reduced, and the annual increment of the plant height is improved.

The invention discloses application of theanine in a preservative and a method for relieving cold injury of respiratory climacteric fruits, and belongs to the technical field of cold injury resistance. Firstly, the invention provides an application of theanine, and the theanine is used for preparing the preservative for relieving the cold injury of the respiratory climacteric fruits. Meanwhile, the theanine aqueous solution is used for preparing the fresh-keeping liquid for relieving the cold injury of the respiratory climacteric fruits. Based on the fresh-keeping liquid, the specific use method comprises the following steps: respectively placing the respiratory climacteric fruits in the theanine solution with the concentration of 5-20 mmol / L, soaking for 5-30 minutes, and drying after soaking is finished. Finally, the invention provides the theanine cold damage resistant preservative film which is prepared from the konjac glucomannan, the chitosan and the theanine, the final concentration of the konjac glucomannan is 0.2-0.8 g / L, the final concentration of the chitosan is 0.2-0.8 g / L, and the final concentration of the theanine is 5-20 mmol L <-1 >. The method is good in cold damage resistance effect and wide in application prospect.

The invention discloses a preservation and refrigeration method of pears, which comprises the following steps: (a) pretreatment after picking, (b) stepped precooling, (c) dynamic controlled atmosphere refrigeration, and (d) regular monitoring of gas components. CaCl2 and tea polyphenol composite treatment is adopted to enhance the cold resistance and disease resistance of fruits, and the metabolic activity is gradually reduced in combination with a three-stage stepped precooling process, so that the preservation and refrigeration effects of the pears are improved; and the respiratory metabolism is precisely regulated and controlled by matching with staged dynamic air-conditioned storage. The method breaks through the bottleneck of the traditional refrigeration technology, prolongs the storage period of the pears to 6-8 months, reduces the rotting rate, is suitable for large-scale commercial preservation, and has remarkable economic value and technical advantages.

The invention relates to the technical field of rice gene engineering, in particular to application of a rice gene OsbZIP21 in regulation and control of cold resistance. The invention discloses an application of a rice gene OsbZIP21 in regulation and control of cold resistance. The nucleotide sequence of the OsbZIP21 gene is as shown in a sequence table SEQ ID NO: 1, and the coded amino acid sequence is as shown in a sequence table SEQ ID NO: 2. According to the invention, the rice OsbZIP21 gene is edited by adopting a CRISPR / Cas9 gene editing technology, the expression of the encoding protein of the rice OsbZIP21 gene is silenced, and two forms of cold-resistant rice mutants are obtained. Experimental results show that under the condition of cold stress, the survival rate of the two obtained rice mutants is obviously higher than that of wild rice mutants, and it is indicated that the OsbZIP21 gene plays an important role in regulation of the cold tolerance of rice. The invention provides new resources and technical means for cold-resistant breeding of rice and other plants.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for early identification of the cold resistance of an actinidia chinensis tree body in the dormancy period, and the SNP molecular marker is located at the Chr69297479 position on the No.6 chromosome of the actinidia chinensis and is closely associated with the cold-resistant phenotype median lethal temperature (LT50) of the actinidia chinensis. The method can be used for identifying the tree body dormancy period cold resistance of natural population and hybrid filial generation of actinidia chinensis. The homozygous genotype (CC) of the SNP locus corresponds to a high-cold-resistance individual in the dormancy period of an actinidia chinensis tree body, and the heterozygous genotype (TC) of the SNP locus corresponds to a low-cold-resistance individual. The cold resistance prediction accuracy in the F1-generation hybrid population reaches 94.94%, and the prediction accuracy in the natural population reaches 96.15%. The molecular marker detection method disclosed by the invention is convenient and rapid, has good specificity and high accuracy, is not influenced by climate, environment and the like, can rapidly and effectively evaluate the cold resistance of natural resources and hybrid filial generations of the actinidia chinensis, and provides powerful technical support for breeding cold-resistant varieties of the actinidia chinensis.

The invention discloses a management method for improving cold resistance and drought resistance of macadamia nuts and increasing flowering quantity, which combines a four-stage dynamic management mode of soil improvement, cold resistance induction, drought resistance and tip control and flowering phase quality improvement, applies an organic fertilizer, a water-retaining agent and a composite slow-release fertilizer, induces root development, slowly releases the fertility of a chemical fertilizer, prolongs the action time of the chemical fertilizer, and improves the yield of the macadamia nuts. Brassinolide and potassium fulvate are compounded to activate cold-resistant gene expression of macadamia nut plants and improve cell membrane stability, so that cold resistance of the macadamia nut plants is improved, melatonin and methyl jasmonate perform double shoot control, an ABA signal channel of the plants is triggered, drought resistance of the plants is improved, flower bud differentiation is promoted, and growth of the macadamia nut plants is promoted. The synergistic effect of alginate oligosaccharide and gamma-aminobutyric acid enhances the elongation ability of a pollen tube, chemical agent stimulation is replaced by biological agent stimulation, the utilization rate of chemical agents is reduced, then the negative influence of the chemical agents on soil is reduced, and finally the fruit setting rate and the sustainable utilization of the soil are improved.

The utility model discloses a low-temperature-resistant ultrafiltration water purification device which comprises a water inlet, a water inlet pump, a primary filter, an ultrafiltration membrane assembly, an anti-freezing glycerol tank, an activated carbon filter, a produced water outlet and a waste liquid discharge pipe, the water inlet is connected with a water inlet pump; the water inlet pump is connected with the primary filter; the primary filter is respectively connected with the anti-freezing glycerol tank and the active carbon filter; the ultrafiltration membrane assembly is connected with the active carbon filter; an ultraviolet sterilizer is arranged between the activated carbon filter and the produced water outlet; the water inlet, the primary filter, the ultrafiltration membrane assembly, the anti-freezing glycerol tank, the activated carbon filter and the ultraviolet sterilizer are all connected with a waste liquid discharge pipe. Enough pipeline pressure is ensured while the water purification effect is ensured; each key node of the device is provided with a pressure relief pipeline connected with a waste liquid discharge pipe, so that the risk caused by excessive pressure is avoided; and the anti-freezing glycerol tank is arranged and is matched with a valve switch to perform pipeline anti-freezing operation, so that the cold resistance of the device is improved.