262 results about "Cold resistance" patented technology

The utility model discloses a low-temperature-resistant ultrafiltration water purification device which comprises a water inlet, a water inlet pump, a primary filter, an ultrafiltration membrane assembly, an anti-freezing glycerol tank, an activated carbon filter, a produced water outlet and a waste liquid discharge pipe, the water inlet is connected with a water inlet pump; the water inlet pump is connected with the primary filter; the primary filter is respectively connected with the anti-freezing glycerol tank and the active carbon filter; the ultrafiltration membrane assembly is connected with the active carbon filter; an ultraviolet sterilizer is arranged between the activated carbon filter and the produced water outlet; the water inlet, the primary filter, the ultrafiltration membrane assembly, the anti-freezing glycerol tank, the activated carbon filter and the ultraviolet sterilizer are all connected with a waste liquid discharge pipe. Enough pipeline pressure is ensured while the water purification effect is ensured; each key node of the device is provided with a pressure relief pipeline connected with a waste liquid discharge pipe, so that the risk caused by excessive pressure is avoided; and the anti-freezing glycerol tank is arranged and is matched with a valve switch to perform pipeline anti-freezing operation, so that the cold resistance of the device is improved.

The invention discloses a pennisetum purpureum PpDREB1D-like gene and application thereof in improving cold resistance and biomass of plants, and relates to the technical field of plant genetic engineering. The PpDREB1D-like gene derived from the pennisetum purpureum is cloned and functionally identified for the first time, and experimental verification shows that the PpDREB1D-like gene can increase the cold resistance of plants, the growth state of the plants under cold stress is remarkably improved through overexpression of the PpDREB1D-like gene, the cold resistance of the plants is remarkably reduced through silence of the PpDREB1D-like gene, and the PpDREB1D-like gene can be used for improving the cold resistance of the plants. A molecular foundation is laid for popularization and planting of pasture and energy crops in cold regions, and the method has remarkable agricultural production and ecological value.

The invention relates to the technical field of analysis and testing, in particular to a method and system for detecting the cold resistance degree of pennisetum purpureum based on spectral imaging, and the method comprises the steps: obtaining and correcting a spectral image of a pennisetum purpureum leaf; determining biochemical values of various induction components, calculating weights by using principal component analysis, and screening out the component with the maximum weight as a target prediction index; extracting the average spectrum data of the region of interest, and constructing a regression prediction model in combination with a target index measured value after preprocessing and characteristic wave band screening; and finally, the index content is inversed by using the model to represent the cold resistance degree. According to the method, key physiological indexes are scientifically screened through principal component analysis, a high-precision non-destructive detection model is established in combination with an optimal spectrum algorithm, and nondestructive, rapid and high-throughput evaluation of the cold resistance of the object grass is realized.

The invention discloses a fertilizing method for cold resistance and yield increase of heading lettuce, and belongs to the technical field of functional water-soluble fertilizers. The method comprises the following steps: applying a bio-organic fertilizer and a ternary compound fertilizer as a base fertilizer in a planting period; topdressing a water-soluble fertilizer A composed of a liquid nitrogen fertilizer and a cold-resistant functional peptide bacterial fertilizer through drip irrigation in the early rosette stage; topdressing a water-soluble fertilizer B consisting of a high-potassium drip irrigation water flush fertilizer and a cold-resistant functional peptide bacterial fertilizer in the later stage of the rosette stage; topdressing high-potassium drip irrigation and water flush fertilizer at the initial stage of the heading stage. On the basis of a water and fertilizer integrated system, the cold-resistant functional peptide bacterial fertilizer is precisely applied in stages, the liquid nitrogen fertilizer and the high-potassium water-soluble fertilizer are optimized, the synergistic effect of peptide substances, functional microorganisms and nutrient elements is fully played, the cold resistance of the heading lettuce is remarkably enhanced, nitrogen is reduced by 15%-20%, the yield is increased by 40%-50%, the income is increased by 1200-1300 yuan per mu, and the yield is increased by 100-200 yuan per mu. And the method has important popularization and application values.

The invention aims to provide a model and a method for identifying the cold resistance of alfalfa in the bud stage, the identification model relates to the relationship between four cold-resistant indexes and cold-resistant grade (Y) of alfalfa at 6 + / -0.1 DEG C, the four cold-resistant indexes are alfalfa germination rate (X1), alfalfa simplified vigor index (X2), alfalfa root length (X3) and alfalfa germination index (X4), and the relational expression is Y =-2.771 + 0.016 X1 + 0.061 X2 + 0.900 X3 + 0.054 X4, when Y is more than or equal to 0.70 and less than or equal to 1.00, the alfalfa variety is a strong cold-resistant variety; when Y is more than 0.40 and less than 0.70, the alfalfa variety is a medium cold-resistant variety; when Y is more than or equal to 0.00 and less than or equal to 0.40, the alfalfa variety is a non-cold-resistant variety. Experiments prove that the model has the characteristics of high accuracy, rapidness and high efficiency when being used for identifying alfalfa cold-resistant germplasm resources.

The invention discloses a composition for enhancing cold resistance based on pyroptosis regulation and a preparation method thereof, and belongs to the technical field of biological medicines. The preparation method of the composition for enhancing cold resistance based on pyroptosis regulation comprises the following steps: S1, respectively preparing an angelica sinensis aqueous extract and an astragalus membranaceus aqueous extract; and S2, mixing the angelica sinensis aqueous extract and the astragalus membranaceus aqueous extract according to the mass ratio of 1: (4-20) to obtain the composition for enhancing cold resistance based on pyroptosis regulation. The invention further provides a composition obtained based on the preparation method, a preparation and application. The composition provided by the invention can significantly prolong the survival time of acute cold exposure mice; the core body temperature of an acute cold exposure mouse is obviously improved, liver injury is relieved, and liver tissue cell pyroptosis pathway protein expression is regulated.

A polyester-based polymer according to the present invention contains 30 mol% or more of cyclohexanedicarboxylic acid, dimethylcyclohexanedicarboxylate, or a combination thereof as a dicarboxylic acid component, and thus is environmentally friendly, has controlled softness, and has excellent quality and processability. In addition, the polyester-based polymer includes a linear C3-C6 alkanediol or the like as a diol component, thereby improving cold resistance, chemical resistance, and elasticity while having softness.

The application provides a white clover surface with super-low ice adhesion strength and a preparation method thereof, which comprises analyzing the relationship between the white clover surface structure and the super-hydrophobic cold resistance; copying the white clover surface structure, and processing periodic micropores on a base material by means of femtosecond laser direct writing; boiling the surface of the periodic micropores; and fluorinating the surface to modify it into a functional surface with super-hydrophobicity. The white clover surface with super-low ice adhesion strength has a micro-nano hierarchical structure, which comprises closely arranged larger micrometer-level periodic pore structures, smaller micrometer-level granular particles dispersedly distributed on the surface of the micropore structures, and densely distributed sub-micrometer-level sheet structures on the surface. The surface has super-hydrophobic low adhesion, the ice adhesion strength is as low as 1.08 kPa, the static icing prolonging time can reach 4 h, the dynamic frost prevention capacity can last for 5 h in a low-temperature and high-humidity environment, and the surface has good wear resistance and reusability.

This invention provides an essential oil composition that combats hypoxia, fatigue, and enhances cold resistance. It is prepared from raw materials in the following weight ratios: 4.0–8.0 parts Rhodiola rosea essential oil, 1.5–2.5 parts ginseng essential oil, 0.5–1.5 parts Angelica sinensis essential oil, and 0.5–1.5 parts Ligusticum chuanxiong essential oil. This invention also provides a method for preparing this composition and its uses. The essential oil composition of this invention can combat hypoxia, fatigue, and enhance cold resistance. It has a fragrant aroma and works rapidly and noticeably. The combination of various essential oils can exert a synergistic effect, making it suitable for special environments such as high altitudes, diving, and aviation, as well as for daily health maintenance and disease prevention.

This invention discloses a method for preparing impact-resistant and cold-resistant polyvinyl chloride (PVC) conduit, belonging to the field of PVC material technology. In this invention, butyl rubber is complexed with dioctyl sebacate and active silica under the initiation of tert-butyl hydrogen peroxide. This complex acts as a flexible dispersed phase in the PVC matrix, effectively absorbing and dissipating impact energy and lowering the glass transition temperature of the material. This allows the conduit to maintain excellent toughness and crack resistance even at low temperatures. Furthermore, it works synergistically with impact modifiers in the system. Ethylene-vinyl acetate copolymer provides elastomer toughening, while maleic anhydride-styrene graft copolymer acts as a highly efficient compatibilizer and interfacial reinforcing agent. The compounded structure forms a toughening network, achieving toughening from molecular chain segment movement to microstructure, thus improving impact strength. Subsequent addition of reinforcing fibers of the same specification further enhances the strength of the conduit. Overall, this results in a PVC conduit with excellent strength, impact and cold resistance, and thermal properties.

The application provides application of a calcium sensing protein ShCBL1 gene and belongs to the technical field of biology.The application overexpresses or superexpresses the ShCBL1 gene in sugarcane or rice, so that the content of proline and superoxide dismutase in the sugarcane or rice is increased, the content of malondialdehyde is reduced, and the cold resistance is improved.Researches show that when the ShCBL1 is superexpressed or overexpressed in plants, the cold stress related physiological indexes such as proline, superoxide dismutase (SOD) and malondialdehyde (MDA) can be significantly affected, so that the cold resistance of the rice and the sugarcane is enhanced.ShCBL1 as a positive regulator of cold resistance regulation can be used for molecular breeding of cold resistance varieties.

The invention discloses wide-temperature-range silicone rubber-based rubber for aerospace thermal protection, a composite material and a preparation method of the wide-temperature-range silicone rubber-based rubber. According to the invention, three monomers, i.e., methylcyclosiloxane, hexaethylcyclotrisiloxane and phenylene silicon monomer, are subjected to ring opening and condensation copolymerization to obtain polysiloxane macromolecules containing three different chain link structures. A phenylene structure is introduced into a silicon rubber main chain, so that the silicon elastomer main chain is limited to form a volatile cyclic oligomer through a chain back-biting reaction and a chain segment rearrangement exchange reaction, the thermal stability is improved, and the initial decomposition temperature is up to 450.16 DEG C; the regularity of a molecular chain is broken by introducing ethyl, so that the glass-transition temperature is as low as 120.2 DEG C, and no crystallization phenomenon occurs. The base adhesive has excellent cold resistance, radiation resistance, ablation resistance, high temperature resistance and the like, and can be used as the base adhesive to be applied to the aspects of aerospace craft protective coatings, dynamic seal putty adhesives, sealants and the like.

This invention discloses KASP markers for assisting in the screening of cold-resistant wheat germplasm and their applications, relating to the field of biotechnology. A method for screening or assisting in the screening of cold-resistant wheat germplasm includes the following steps: detecting whether the genotype of the wheat to be tested is qFR-7A.1a or qFR-7A.1b; wheat with genotype qFR-7A.1a exhibits stronger or potentially stronger cold resistance than wheat with genotype qFR-7A.1b; the wheat with genotype qFR-7A.1a is a GG homozygous genotype based on the G101A SNP site; the wheat with genotype qFR-7A.1b is an AA homozygous genotype based on the G101A SNP site; the G101A SNP site is the 101st nucleotide from the 5' end of SEQ ID NO: 1 in the wheat genome. This invention has significant theoretical and economic value for using molecular markers to assist in the selection of wheat germplasm or breeding progeny materials with high cold resistance.

Provided is a millable silicone rubber compound excellent in cold resistance, which can maintain rubber elasticity in an ultra-low temperature range without using various conventional techniques for improving cold resistance. 【Solution means】A millable silicone rubber compound containing the following components (A) to (E). (A) The following formula (1) M n M Vi (2-n) D γ D Vi δ (1) M:R 1 3SiO (1 / 2) M Vi :R 1 2RSiO (1 / 2) D:R 1 2SiO (2 / 2) D Vi :R 1 RSiO (2 / 2) (In the formula, R is independently an alkenyl group, R 1 is independently an unsubstituted or substituted monovalent hydrocarbon group not containing an aliphatic unsaturated bond. n is a positive number from 0 to 2. γ satisfies (γ / (γ + δ)) ≤ 0.98, δ satisfies (δ / (γ + δ)) ≥ 0.02, and (γ + δ) is a positive number such that (γ + δ) ≥ 6000.) raw rubber represented by (B) A compound having one hydrogen atom bonded to a silicon atom, (C) A specific dispersant, (D) Reinforcing silica having a specific specific surface area, (E) An addition reaction catalyst

The invention relates to an oil seal cold resistance test work fixture, and relates to the field of cold resistance verification of mechanical sealing elements, the oil seal cold resistance test work fixture is used for testing abnormal phenomena generated when an oil seal is non-uniformly stressed, the oil seal cold resistance test work fixture comprises a base, a deviation assembly and a test assembly, the deviation assembly comprises a deviation seat, a bearing seat, a deviation piece and an outer ring, the deviation seat and the base are movably arranged, and the bearing seat and the outer ring are movably arranged. The offset seat is provided with an offset groove, the bearing seat is provided with an offset block, the offset block is arranged in the offset groove in a sliding mode, the offset piece can drive the bearing seat to move, the outer ring and the bearing seat are fixed, and the outer ring is fixedly provided with the oil seal. The testing assembly comprises a flange and a testing column, the flange is rotationally connected with the base, and part of an oil seal lip of the oil seal abuts against the testing column; the axial direction of the test column is perpendicular to the moving direction of the bearing seat; according to the structure, the quality of the sealing lip of the oil seal can be effectively inspected and controlled in the cold resistance test of the oil seal.

The invention relates to the field of agricultural biochemical engineering and plant protection, and particularly discloses a liquid film-forming agent for preventing crops from freezing injury due to late spring coldness and a preparation method of the liquid film-forming agent. The film-forming agent is prepared from carboxymethyl chitosan, sodium carboxymethyl cellulose, kasugamycin, brassinolide, glycol and glycerol mixed dihydric alcohol, Tween 80 and deionized water according to a specific proportion. The preparation method comprises the key processes of step-by-step dissolution, mixing, functional component addition and the like. Organic fusion of physical barrier, ice nucleus inactivation and physiological regulation is realized through multiple action mechanisms of natural polymer composite film forming, biopesticide targeted sterilization, plant hormone induced cold resistance and dihydric alcohol synergistic antifreezing. The product can provide efficient protection at a low temperature of-4 DEG C, significantly reduces the frost damage rate, has the characteristic of 28-day natural rapid degradation, and solves the technical bottlenecks of single function, difficult degradation and poor environmental compatibility of a traditional film agent.

The invention discloses an SNP (Single Nucleotide Polymorphism) marker related to the body size and temperature adaptability of worker bees of apis cerana and application of the SNP marker. According to the invention, 25 key SNP sites and 10 associated genes for regulating and controlling the body type and temperature adaptation of the apis cerana are screened by a selection signal analysis method. Research finds that in the second intron region (6452066th site of the chromosome 7 of the bees) of the Foxo gene, the mutation type is T / C, and the mutation type is closely related to the body size and temperature adaptability of the Chinese bees. Through verification, in Hainan apis cerana and Aba apis cerana with extremely different body sizes and living environment temperatures, mutation position genotypes are different, TT is a large-body cold-resistant dominant genotype, and CC / TC is a small-body heat-resistant dominant genotype; a gene expression quantity detection result shows that the expression quantity of the Foxo gene is in negative correlation with a large body type and cold resistance and is in positive correlation with a small body type and heat resistance. The method can be used for genetic improvement of bee body shape characters and has breeding application value.

The application discloses a kind of high weatherability polyvinyl chloride cable material and preparation method thereof, including the following weight parts of component: PVC resin 100 parts;Plasticizing system 35-55 parts;Dynamic crosslinking toughening compound 8-20 parts;Calcium-zinc stabilizer 4-8 parts;Ultraviolet light stabilizing system 0.3-1.5 parts;Flame-retardant-reinforcing synergic system 30-60 parts;Processing lubricating system 1-3 parts.The application constructs dynamic reversible ionic crosslinking network in PVC cable material for the first time, and simultaneously solves the two big problems of small molecule plasticizer migration and low temperature brittleness, unifies the inherent contradiction of cold resistance and anti-migration;Gradient refractive index core-shell toughening agent is designed and applied as "structured ultraviolet trap", and the ultraviolet absorption efficiency is greatly improved through physical optical mechanism, realizes the synergy of toughening and super long weather resistance, and does not depend on excessive carbon black, which provides design space for product color.

The invention discloses an oil-resistant cold-resistant flame-retardant polyvinyl chloride material and a preparation method thereof. The polyvinyl chloride material is prepared from the following raw materials in parts by weight: 100 parts of PVC resin, 40-60 parts of a plasticizer, 30-50 parts of a flame retardant, 15-25 parts of a cold-resistant agent, 10-20 parts of an oil-resistant agent, 5-10 parts of a heat stabilizer and 2-5 parts of a lubricant. Wherein the flame retardant is a composite flame retardant composed of aluminum hydroxide, magnesium hydroxide and antimony trioxide, the cold-resistant agent is an ethylene terpolymer, and the oil-resistant agent is a polar copolymer of acrylonitrile and butadiene. The material is excellent in comprehensive performance, the low-temperature impact performance can pass minus 40 DEG C, after an oil resistance test, the mechanical property loss is extremely small, the oxygen index is maintained to be about 30%, the stable mechanical property and thermal stability are achieved, collaborative optimization of oil resistance, cold resistance and flame retardance is achieved, and the service life of the material is prolonged. The cable is suitable for cable preparation under complex working conditions such as automobile engine cabins, industrial equipment in extremely cold areas and petrochemical engineering, the preparation process is high in controllability, and industrial production is easy.

The utility model provides a highly cold-resistant flexible cable, which comprises an inner sheath and a cable core arranged in the inner sheath, a reinforcing layer, a shielding layer, an armor layer, an outer insulating layer and an outer sheath are sequentially arranged outside the inner sheath from inside to outside, and a filling layer is arranged between the inner sheath and the cable core; an inner core, a support frame and a heating body are also arranged in the inner sheath; the supporting frame is a plurality of regular polygon pipes which are arranged along the length direction of the inner core and are sleeved outside the inner core, each side edge of the supporting frame is concave inwards to be arc-shaped, the side edges of the supporting frame correspond to the cable cores one by one and are mutually attached together, and the inner wall of each side edge of the supporting frame is in contact with the inner core; the multiple heating bodies are arranged in the supporting frame in a sleeved mode. The cold resistance of the cable is improved, so that the cable is suitable for an extremely low-temperature environment; by arranging the support frame in the middle of the cable, the radial shrinkage resistance of the cable is improved; the operation temperature of the cable is improved through the heating body, attenuation of cable material performance is slowed down, and it is ensured that the cable has a long service life in a low-temperature environment.

The invention discloses application of a SbMYB113-like gene to improvement of low-temperature-resistant breeding of scutellaria baicalensis, belongs to the technical field of plant biology, is used for scutellaria baicalensis, and is characterized in that the nucleotide sequence of the SbMYB113-like gene is shown as SEQ ID NO: 1, by reducing overexpression of the SbMYB113-like gene in the scutellaria baicalensis, the anti-oxidation defense system of the scutellaria baicalensis is improved, the stability of the cell structure and function is protected, and the stability of the low-temperature-resistant breeding of the scutellaria baicalensis is improved. The tolerance of the scutellaria baicalensis to low-temperature stress is improved. The invention aims at researching subcellular localization of SbMYB113-like protein, analyzing tissue specific expression and a low-temperature stress response expression mode of the SbMYB113-like gene in combination with real-time fluorescent quantitative PCR (Polymerase Chain Reaction), and verifying the cold resistance function of SbMYB113-like through genetic transformation, so that the SbMYB113-like gene has an application value in low-temperature scutellaria baicalensis breeding.

This invention belongs to the field of agricultural biotechnology, specifically relating to a type of sisal. DREB Genes and their application in improving plant cold resistance. This invention provides sisal. DREB The gene expression level increased significantly after low-temperature stress, indicating a close relationship between its expression and the response of sisal to low-temperature stress. The study found that transgenic methods can significantly improve the expression level of sisal. DREB Genes were overexpressed, along with sisal DREB Increased gene expression levels led to improved cold resistance in transgenic Arabidopsis thaliana. Overexpression of sisal in plants also improved cold resistance. DREB The discovery that genes can enhance plant cold resistance can be used to breed new varieties or seedlings of cold-resistant plants, providing a theoretical basis and genetic resources for genetic breeding, which is of great significance to agricultural production.

The invention relates to the technical field of ecological restoration materials, in particular to an ecological restoration material based on inorganic-organic compounding and a preparation method thereof. The ecological restoration material is prepared from the following raw materials in parts by weight: 55 to 65 parts of crushed coal gangue, 40 to 45 parts of Portland cement, 15 to 20 parts of polyacrylamide, 8 to 12 parts of starch, 25 to 30 parts of citric acid leached fly ash, 20 to 25 parts of organic matter, 7 to 11 parts of mica powder-doped improved effect comprehensive agent, 5 to 8 parts of multi-conditioning multi-feeding agent, 4 to 6 parts of silane coupling agent KH560, 2 to 4 parts of passivating agent, 1 to 3 parts of chelating agent and 30 to 35 parts of deionized water. The prepared ecological restoration material has excellent organic matter improvement performance and heavy metal passivation effect, meanwhile, the vegetation planting effect, the product strength, the anti-erosion effect and the freezing and cold resistance effect are remarkable, and the comprehensive coordination effect of the product is excellent.

To provide a plasticizer capable of obtaining a thermoplastic resin composition excellent in flexibility, cold resistance and heat resistance.SOLUTION: The plasticizer contains a (meth) acrylic polymer having a weight average molecular weight of 3,000 or less. The (meth) acrylic polymer includes a structural unit (U1) derived from an alkyl (meth) acrylate and a structural unit (U2) having a cyclic ether group having 3 to 5 ring atoms, and has an SP value of 9.30 or more and a glass-transition temperature of - 75 °C. or less.SELECTED DRAWING: None

The invention discloses a comprehensive nutrition regulation and control method for improving overwintering cold resistance of citrus fruit trees, and relates to the field of fruit tree cultivation, and the method comprises the following steps: S1, soil conditioning and mycorrhiza inoculation after fruit picking: determining the content of rapidly available phosphorus in orchard soil, when the content of rapidly available phosphorus is greater than 30mg / kg, firstly applying humic acid and calcium magnesium phosphate fertilizer for phosphorus reduction treatment, and after the content of rapidly available phosphorus is reduced to be less than or equal to 20mg / kg, then applying a nutrient solution; digging radial short ditches on the inner side of a water drip of a tree crown, mixing a pre-activated arbuscular mycorrhizal fungus inoculant with an organic carrier, filling the mixture into the ditches, and earthing and watering; according to the comprehensive nutrition regulation and control method for improving the overwintering cold resistance of the citrus fruit trees, through soil phosphorus pre-regulation and local enrichment inoculation methods, the mycorrhizal fungus colonization rate is increased to 50% or above from less than 20%, and the mycorrhizal inoculation effect and the input-output ratio are remarkably improved.

The invention discloses application of a tomato gene SlZFP2 as a negative regulation factor in regulation of plant cold resistance, belongs to the technical field of plant genetic engineering, and finds and confirms that the tomato zinc finger protein gene SlZFP2 is a key negative regulation factor of plant cold resistance for the first time. Experiments show that the expression of the SlZFP2 is induced by low temperature, the overexpressed transgenic tomato plant shows more serious cold injury symptoms under low temperature stress, the photosynthetic efficiency is reduced, active oxygen (H2O2 and O2-) is excessively accumulated, and the activity of antioxidant enzymes (SOD, POD and CAT) is weakened; the overexpression of the SlZFP2 can inhibit the biosynthesis and signal transduction of abscisic acid (ABA), and down-regulate the expression of cold response genes SlGRAS4 and SlCBF1; based on the discovery, the invention provides an application of using the SlZFP2 gene or an expression product thereof as a molecular marker for screening tomato materials with different cold resistance, creating a transgenic plant with reduced cold resistance by overexpressing the SlZFP2, and providing a recombinant DNA construct containing an SlZFP2 coding sequence and a host cell.

The utility model relates to the field of wheat indoor identification, in particular to a temperature and light adjustable wheat cold resistance indoor identification platform capable of eliminating position effect, which comprises a fixed frame, rubber and plastic heat preservation plates are arranged on the left side and the right side of the fixing frame respectively, the same rubber and plastic heat preservation plates are arranged at the rear end of the fixing frame, windproof polyester cloth is arranged at the front end of the fixing frame, breathable gauze is arranged at the top of the fixing frame, a supporting frame is arranged at the top of the fixing frame, and a nylon gauze is arranged on the outer surface of the supporting frame. A plurality of round holes are formed in the top of the nylon gauze element; airflow disturbance is reduced through the windproof polyester fabric on the front face, a buffer layer is formed by combining the double-layer ceiling design to reduce vertical wind speed fluctuation, meanwhile, the rotating disc drives plants to rotate periodically, and the microenvironment difference between the edge and the center is eliminated; the temperature control of natural low-temperature planting or a simple incubator is more accurate, the wind speed can be controlled, and the large environmental fluctuation and the position effect are reduced.

This invention relates to the field of biotechnology, specifically to... StNCED3 The application of genes in improving the abiotic resistance of potatoes: This invention involves constructing... StNCED3 The screening and identification of gene overexpression vectors and transgenic lines, and their application in improving potato resistance to drought and low-temperature stress, revealed the overexpression of... StNCED3 The gene can significantly enhance the drought and cold resistance of potato plants, providing core functional genes and high-quality breeding materials for molecular breeding of potatoes for drought and cold resistance. It effectively solves the technical bottlenecks of weak drought and cold resistance of the current main potato varieties and unstable yield in arid and low-temperature production areas, and has important theoretical research value and industrial application prospects.

This invention relates to the field of plant molecular biology, specifically to a loquat fatty acid desaturase-related gene, EjFAD8, its encoded protein, and its applications. The full-length cDNA sequence of this gene is shown in SEQ ID No. 1, and the amino acid sequence of its encoded protein is shown in SEQ ID No. 2. The EjFAD8 gene of this invention increases plant cold resistance by increasing the synthesis of SQDG (16:0 / 18:1) and promoting the expression of related genes in the ICE-CBF-COR low-temperature stress pathway. Using Agrobacterium tumefaciens Gv3101-mediated flower-dip method, the plant expression vector pCambia2300-35S::EjFAD8-eGFP containing the target gene was transformed into wild-type Arabidopsis thaliana. Overexpression of 35S::EjFAD8 in the transgenic Arabidopsis enhanced its cold resistance. This invention utilizes transgenic Arabidopsis thaliana plant materials obtained from the loquat EjFAD8 gene. Under low temperature stress, these plants can increase the content of unsaturated fatty acids, enhance the expression of low temperature-related genes, and reduce the content of H2O2 in the plant, thereby increasing the plant's cold resistance and showing great application potential.

The invention discloses a sea-buckthorn-derived T5H gene and application of an encoding protein thereof in improving the cold resistance of plants, and belongs to the technical field of biology. The hippophae rhamnoides-derived T5H gene and the encoded protein thereof can enhance the survival rate and cold resistance of plants (such as crops) under low-temperature stress, and have wide application prospects and popularization values in agricultural production practice.