469 results about "Cold resistance" patented technology

The invention discloses a high-yield cold-resistant pennisetum germplasm screening method and application thereof, and the method is based on 10 yield traits, 4 cold-resistant indexes and 4 low-temperature photosynthetic ability indexes of pennisetum, constructs a multi-dimensional dynamic monitoring system across autumn, winter and spring, and performs high-yield cold-resistant comprehensive evaluation on the pennisetum germplasm, thereby screening out a high-yield cold-resistant breeding material. The invention provides a comprehensive, efficient and accurate high-yield cold-resistant Chinese pennisetum germplasm screening method, through a multi-dimensional dynamic monitoring system crossing autumn, winter and spring, yield traits, cold resistance indexes and low-temperature photosynthetic ability indexes are integrated, and the problems that a traditional method is single in evaluation dimension, disjointed with field reality and insufficient in dynamic adaptability are solved. The comprehensive evaluation system based on the principal component quantitative model not only fills the blank of the high-yield and cold-resistant evaluation model of the pennisetum alopecuroides, but also remarkably improves the breeding efficiency, provides powerful support for cultivating cold-resistant and yield-reduction-free breakthrough varieties, and has important application value and popularization prospect.

The invention discloses a PgEGY3 gene for improving cold resistance of pennisetum alopecuroides and increasing fresh weight of leaves and application of the PgEGY3 gene, and relates to the technical field of genetic engineering, and the nucleotide sequence of the PgEGY3 gene of the pennisetum alopecuroides is shown as SEQ ID NO.1. The invention also discloses a protein coded by the Pennisetum alopecuroides PgEGY3 gene. The invention also discloses a recombinant expression vector and a recombinant host cell comprising the Pennisetum alopecuroides PgEGY3 gene. The invention also discloses application of the PgEGY3 gene, a recombinant expression vector and a recombinant host cell in improving cold resistance and biomass of pennisetum alopecuroides. It is verified that the Pennisetum alopecuroides PgEGY3 gene can improve the cold resistance of plants, and the growth state of the plants under cold stress is remarkably improved through overexpression of the PgEGY3 gene.

The invention discloses a potato cold resistance identification method and system, and relates to the technical field of cold resistance identification. The method comprises the following steps: cultivating potato tissue culture seedlings in a standardized manner, and randomly dividing the seedlings into a low-temperature stress group and a normal-temperature control group; performing standardized low-temperature treatment including slow descent and constant-temperature stages on the stress group; the method comprises the following steps: acquiring multi-source data such as chlorophyll fluorescence parameters, canopy thermal imaging, digital image phenotypes and biochemical indexes, and forming a unified data set after derivative index calculation and standardized fusion; inputting the fusion data into an improved neural network model, training the model through a particle swarm optimization algorithm, and outputting a quantitative potato cold resistance index; automatically evaluating the cold resistance grade according to the potato cold resistance index and generating an identification report; standardization of the identification process, fusion analysis of multi-dimensional indexes and intelligent result analysis are achieved, and the accuracy, efficiency and practicability of potato cold resistance identification are remarkably improved.

The invention discloses a cold response PaIBH1 protein and application of a coding gene thereof in enhancing cold stress resistance of plants. The cold response PaIBH1 protein is a protein PaIBH1 with an amino acid sequence as shown in SEQ ID NO: 2. Experiments prove that the gene for coding the protein PaIBH1, namely the gene PaIBH1, is overexpressed in wild type arabidopsis thaliana, so that the cold resistance of the arabidopsis thaliana can be improved; the improvement of the cold resistance shows that after cold treatment, the fresh weight of the overground part is increased, the wilting rate is reduced, the leaf membrane damage and electrolyte leakage are reduced, the photosynthetic ability is improved, the membrane lipid peroxidation damage is relieved, the active oxygen scavenging ability is enhanced and / or the active oxygen accumulation in the plant body is reduced. Therefore, the protein PaIBH1 can improve the cold resistance of the plants. The method has a very wide application prospect in the aspect of cold-resistant genetic improvement of crops or pasture.

The invention belongs to the technical field of plant molecular biology, and relates to application of VDAC1 / 3a allele in regulation of tomato cold resistance. According to the invention, the expression quantity of the SlVDAC1 / 3a gene in the VDAC1 / 3a allele is reduced by means of knockout or mutation and the like, so that the cold resistance of the tomato can be enhanced, the yield of the tomato and the content of soluble solids are not negatively influenced, and a theoretical basis and gene resources are provided for cultivating and screening cold-resistant tomato varieties.

The invention belongs to the technical field of agricultural remote sensing, and discloses a method for evaluating cold resistance of pennisetum alopecuroides based on unmanned aerial vehicle multispectral images, which comprises the following steps: acquiring agronomic characters related to cold resistance, such as chlorophyll content, overwintering rate and the like of the pennisetum alopecuroides before and after low-temperature stress through manual measurement, and acquiring the multispectral images by using an unmanned aerial vehicle; the image is preprocessed, and various vegetation indexes such as DVI, NDVI and RVI are extracted; on the basis of agronomic character data, a comprehensive cold resistance index CRI is constructed by adopting principal component analysis and a membership function method; using the vegetation index as an independent variable and CRI as a dependent variable, and using a machine learning algorithm to construct a prediction model; and finally, realizing rapid and nondestructive evaluation on the cold resistance of a large-range pennisetum alopecuroides group by utilizing the model, and outputting a five-level cold resistance grading result according to a CRI value. According to the method, the problems of low efficiency and high difficulty of traditional manual evaluation are solved, high-throughput and precise cold resistance identification is realized, and reliable technical support is provided for cold-resistant breeding and cultivation management of pennisetum alopecuroides.

The invention discloses a KASP marker for auxiliary screening of cold-resistant wheat germplasm and application of the KASP marker, and relates to the technical field of biology, in particular to a method for screening or auxiliary screening of wheat cold-resistant germplasm, which comprises the following steps: detecting whether the genotype of wheat to be detected is genotype qFR-7A. 1a or genotype qFR-7A. 1b; the cold resistance of the wheat with the genotype qFR-7A. 1a is higher than that of the wheat with the genotype qFR-7A. 1b, or the cold resistance of the wheat with the genotype qFR-7A. 1a is higher than that of the wheat with the genotype The wheat of which the genotype is qFR-7A. 1a is wheat of which the genotype is GG homozygous based on a G101A SNP (Single Nucleotide Polymorphism) site; the wheat of which the genotype is qFR-7A. 1b is wheat of which the genotype is AA homozygous based on a G101A SNP (Single Nucleotide Polymorphism) site; the G101A SNP site is the 101 nucleotide from the 5'terminal of SEQ ID NO: 1 in a wheat genome. The molecular marker has important theoretical significance and economic value for molecular marker-assisted selection of wheat germplasm or breeding offspring materials with relatively high cold resistance.

The invention belongs to the technical field of sealing materials, and particularly relates to a rubber composite sealing material and a liquid phase preparation method and application thereof. According to the liquid-phase preparation method of the rubber composite sealing material, provided by the invention, based on deep understanding of a liquid-phase synthesis process of first liquid-phase pre-dispersion and then condensation, a modified carbon black dispersion liquid, a modification auxiliary agent and a modified synthetic rubber latex dispersion liquid are prepared, so that carbon black and the auxiliary agent can be promoted to be uniformly and stably dispersed in a synthetic rubber system; the dispersion uniformity of the carbon black and the auxiliary agent is improved, so that the vulcanized rubber composite sealing material is not easy to have insufficient or excessive local crosslinking or structural defects, thereby improving the mechanical property, air tightness and cold resistance of the rubber composite sealing material, and being a high-performance rubber composite material; the technical problem that a high-performance rubber material is difficult to prepare by an existing preparation method is solved.

The invention relates to a Paenibacillus polymyxa strain B01 and application of the Paenibacillus polymyxa strain B01, and belongs to the technical field of microorganisms. The paenibacillus polymyxa B01 disclosed by the invention is preserved in the Guangdong Microbial Culture Collection Center, and the preservation number of the paenibacillus polymyxa B01 is GDMCC NO.66594. The strain is obtained by separation, purification and screening from a soil sample, and tests show that the strain has excellent pH and high temperature tolerance; antibacterial active substances generated by fermentation of the bacillus subtilis have a relatively good antibacterial effect on escherichia coli, pseudomonas aeruginosa, pseudomonas putida, staphylococcus aureus, candida albicans, aspergillus brazilis, propionibacterium acnes and malassezia; and the antibacterial active substance has excellent heat resistance, cold resistance, illumination stability, pH stability and preservative effect, belongs to a mild low-irritation raw material, can be used for preparing daily chemical products and medicines, and has wide application prospects and important conversion research values.

The utility model belongs to the technical field of cables, and particularly relates to a crosslinked polyethylene nano-composite power cable, which comprises a cable core, an insulating layer is arranged on the outer side of the cable core, and a buffer layer is filled between the insulating layer and the cable core; a flame-retardant layer is arranged on the outer side of the shielding layer, and a heat preservation layer is arranged on the outer side of the flame-retardant layer. By arranging the insulating layer, the shielding layer, the flame-retardant layer, the heat preservation layer, the anti-freezing layer and the wear-resistant layer, protection on the cable core can be effectively enhanced, and the cable has excellent cold resistance, good insulating performance, good flame retardance, improved wear resistance and pressure resistance when in use; therefore, the service life of the cable is prolonged.

A method for improving degradation efficiency of corn straw at low temperature comprises the steps that cell-free culture fluid PfCFs prepared from Pseudomonas fragi D12 participates in fermentation and degradation of the corn straw, the classification name of the strain D12 is Pseudomonas fragi D12, the strain D12 is preserved in China Center for Type Culture Collection, the preservation address is No. 299, 8th road, Wuchang District, Wuhan City, Hubei Province, China, and the preservation number is CGMCC NO. The preservation number is CCTCC (China Center For Type Culture Collection) NO: M2025126, and the preservation date is January 14, 2025. The acellular culture solution of the pseudomonas rubrassicae D12 can promote the growth of various normal-temperature bacteria and fungi functional bacteria at low temperature and improve the cold resistance of the functional bacteria, and CFS of the pseudomonas rubrassicae D12 also contains related enzymes capable of degrading lignin; at a low temperature, the PfCFs synchronously promotes the co-growth of bacteria bacillus subtilis and fungi Irpex lacteus, so that the degradation efficiency of the functional bacteria on lignocellulose in the corn straws is improved.

The invention relates to the technical field of plant genetic engineering, in particular to application of a rice OsERF141 protein and a coding gene thereof in improving low-temperature tolerance of plants. Experiments show that the low-temperature resistance of an OsERF141 function deletion mutant strain is remarkably reduced, and the low-temperature resistance of an overexpressed OsERF141 transgenic strain is remarkably improved. The invention provides a new gene resource for the cold resistance problem of rice, and has important significance for the molecular mechanism research of low-temperature stress and the cultivation of new low-temperature-resistant varieties.

The invention provides a molecular marker composition related to rice seedling stage cold resistance, a primer composition, a kit and application thereof, and belongs to the technical field of crop genetic breeding. The invention provides a molecular marker composition related to rice seedling stage cold tolerance. The molecular marker composition comprises at least one of a first SNP marker, a second SNP marker, a third SNP marker, a fourth SNP marker, a fifth SNP marker and an InDel marker. The invention further provides a primer composition for detecting the cold resistance of the rice in the seedling stage. The primer composition comprises at least one of a first primer group, a second primer group, a third primer group, a fourth primer group, a fifth primer group and an InDel detection primer group. The molecular marker composition and the primer composition disclosed by the invention can be used for accurately distinguishing a cold-tolerant rice variety and a cold-sensitive rice variety in a seedling stage, and scientific guidance is provided for breeding and improvement of a new cold-tolerant rice variety.

The invention discloses application of theanine in a preservative and a method for relieving cold injury of respiratory climacteric fruits, and belongs to the technical field of cold injury resistance. Firstly, the invention provides an application of theanine, and the theanine is used for preparing the preservative for relieving the cold injury of the respiratory climacteric fruits. Meanwhile, the theanine aqueous solution is used for preparing the fresh-keeping liquid for relieving the cold injury of the respiratory climacteric fruits. Based on the fresh-keeping liquid, the specific use method comprises the following steps: respectively placing the respiratory climacteric fruits in the theanine solution with the concentration of 5-20 mmol / L, soaking for 5-30 minutes, and drying after soaking is finished. Finally, the invention provides the theanine cold damage resistant preservative film which is prepared from the konjac glucomannan, the chitosan and the theanine, the final concentration of the konjac glucomannan is 0.2-0.8 g / L, the final concentration of the chitosan is 0.2-0.8 g / L, and the final concentration of the theanine is 5-20 mmol L <-1 >. The method is good in cold damage resistance effect and wide in application prospect.

The invention relates to the technical field of rice gene engineering, in particular to application of a rice gene OsbZIP21 in regulation and control of cold resistance. The invention discloses an application of a rice gene OsbZIP21 in regulation and control of cold resistance. The nucleotide sequence of the OsbZIP21 gene is as shown in a sequence table SEQ ID NO: 1, and the coded amino acid sequence is as shown in a sequence table SEQ ID NO: 2. According to the invention, the rice OsbZIP21 gene is edited by adopting a CRISPR / Cas9 gene editing technology, the expression of the encoding protein of the rice OsbZIP21 gene is silenced, and two forms of cold-resistant rice mutants are obtained. Experimental results show that under the condition of cold stress, the survival rate of the two obtained rice mutants is obviously higher than that of wild rice mutants, and it is indicated that the OsbZIP21 gene plays an important role in regulation of the cold tolerance of rice. The invention provides new resources and technical means for cold-resistant breeding of rice and other plants.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker for early identification of the cold resistance of an actinidia chinensis tree body in the dormancy period, and the SNP molecular marker is located at the Chr69297479 position on the No.6 chromosome of the actinidia chinensis and is closely associated with the cold-resistant phenotype median lethal temperature (LT50) of the actinidia chinensis. The method can be used for identifying the tree body dormancy period cold resistance of natural population and hybrid filial generation of actinidia chinensis. The homozygous genotype (CC) of the SNP locus corresponds to a high-cold-resistance individual in the dormancy period of an actinidia chinensis tree body, and the heterozygous genotype (TC) of the SNP locus corresponds to a low-cold-resistance individual. The cold resistance prediction accuracy in the F1-generation hybrid population reaches 94.94%, and the prediction accuracy in the natural population reaches 96.15%. The molecular marker detection method disclosed by the invention is convenient and rapid, has good specificity and high accuracy, is not influenced by climate, environment and the like, can rapidly and effectively evaluate the cold resistance of natural resources and hybrid filial generations of the actinidia chinensis, and provides powerful technical support for breeding cold-resistant varieties of the actinidia chinensis.

The utility model discloses a low-temperature-resistant ultrafiltration water purification device which comprises a water inlet, a water inlet pump, a primary filter, an ultrafiltration membrane assembly, an anti-freezing glycerol tank, an activated carbon filter, a produced water outlet and a waste liquid discharge pipe, the water inlet is connected with a water inlet pump; the water inlet pump is connected with the primary filter; the primary filter is respectively connected with the anti-freezing glycerol tank and the active carbon filter; the ultrafiltration membrane assembly is connected with the active carbon filter; an ultraviolet sterilizer is arranged between the activated carbon filter and the produced water outlet; the water inlet, the primary filter, the ultrafiltration membrane assembly, the anti-freezing glycerol tank, the activated carbon filter and the ultraviolet sterilizer are all connected with a waste liquid discharge pipe. Enough pipeline pressure is ensured while the water purification effect is ensured; each key node of the device is provided with a pressure relief pipeline connected with a waste liquid discharge pipe, so that the risk caused by excessive pressure is avoided; and the anti-freezing glycerol tank is arranged and is matched with a valve switch to perform pipeline anti-freezing operation, so that the cold resistance of the device is improved.

The invention discloses an application of an alfalfa gene MsCBF4 in improving the cold resistance of plants, and belongs to the technical field of gene engineering. In order to excavate a gene with a clear anti-freezing function from medicago sativa, the medicago sativa gene MsCBF4 is successfully cloned, and the expression of the MsCBF4 gene is found to be induced by cold stress and freezing stress through the expression specificity and space-time expression analysis of the gene in the medicago sativa. Besides, a plant overexpression vector containing the alfalfa MsCBF4 gene and recombinant agrobacterium are constructed, transgenic arabidopsis thaliana overexpressing the MsCBF4 gene is obtained through a genetic transformation experiment, freezing tolerance analysis on the transgenic arabidopsis thaliana overexpressing the MsCBF4 gene shows that the overexpression of the MsCBF4 gene enhances the tolerance of the arabidopsis thaliana to freezing stress, and the transgenic arabidopsis thaliana overexpressing the MsCBF4 gene can be used for preventing freezing stress. Therefore, the MsCBF4 gene can be used for improving the cold resistance of the plant.

The invention discloses application of a tea tree CsNAC25 gene in negative regulation of cold resistance of plants, the gene belongs to a tea tree NAC transcription factor family, and the nucleotide sequence of the gene is shown as SEQ ID NO.1. The tea tree CsNAC25 gene negatively regulates the cold resistance of the tea tree, and the gene expression is reduced under low temperature induction. The over-expression of the gene in tobacco weakens the cold resistance of a transgenic plant, and the expression of CsNAC25 is silenced by adopting a VIGS technology in a tea tree, so that the cold resistance of the tea tree is remarkably improved. Silencing CsNAC25 effectively reduces the wilting degree and the ion leakage rate of tea seedling leaves under low temperature stress, activates a key CBF signal channel and promotes accumulation of osmoregulation substances. The application of the application is expected to effectively improve the cold resistance when low-temperature disasters such as late spring coldness occur, and has important theoretical significance and wide application prospects for guaranteeing the stable yield and quality of the tea industry.

The invention provides a pogostemon cablin cold stress response transcription factor and application thereof in resisting cold and increasing pogostemon cablin alcohol content, and belongs to the technical field of gene engineering. The terpene metabolism cold response transcription factor PatbZIP6 and / or PatMYB5 is obtained through screening, the transcription factor obtained through screening can remarkably improve synthesis accumulation of patchouli alcohol through a genetic transformation method, the cold resistance of patchouli is improved, and the transcription factor can be used for breeding new varieties of patchouli.

The application of exogenous complex control measures in reducing tobacco low temperature early flowering, after tobacco transplanting, before the arrival of low temperature cold damage, exogenous complex measures solution is sprayed; the exogenous complex chemical measures solution is the mixed solution of magnesium sulfate and salicylic acid. The invention uses low temperature sensitive tobacco variety Yunyan 87 as experimental material, compares and analyzes the changes of leaf phenotype, physiology and biochemistry indexes of Yunyan 87 seedlings under low temperature stress and various exogenous material treatment, and confirms the role of the complex measures in resisting cold stress. The low temperature adversity resistance of tobacco seedling stage plants is improved by using suitable concentration magnesium sulfate to spray the tobacco seedlings, so that the growth state of the tobacco seedlings subjected to the low temperature adversity stress of the reverse spring cold tends to be normal, the frost injury and frost death phenomenon of the tobacco seedlings after the cold stress is reduced, the cold resistance of the tobacco seedling stage is improved, the early flowering in the later period is prevented, and the operation is simple, the cost is low, and the effect is obvious.

The invention discloses a pennisetum purpureum PpDREB1D-like gene and application thereof in improving cold resistance and biomass of plants, and relates to the technical field of plant genetic engineering. The PpDREB1D-like gene derived from the pennisetum purpureum is cloned and functionally identified for the first time, and experimental verification shows that the PpDREB1D-like gene can increase the cold resistance of plants, the growth state of the plants under cold stress is remarkably improved through overexpression of the PpDREB1D-like gene, the cold resistance of the plants is remarkably reduced through silence of the PpDREB1D-like gene, and the PpDREB1D-like gene can be used for improving the cold resistance of the plants. A molecular foundation is laid for popularization and planting of pasture and energy crops in cold regions, and the method has remarkable agricultural production and ecological value.

The invention discloses cold-resistant and tear-resistant coating resin and a preparation method thereof, and relates to the technical field of coatings. The invention discloses cold-resistant and tear-resistant coating resin. The invention relates to a water-based paint, which is prepared from the following raw materials in parts by weight: 40 to 60 parts of modified epoxy resin, 15 to 25 parts of poly (hexanediol adipate), 5 to 10 parts of modified nano silicon dioxide, 8 to 15 parts of diphenylmethane diisocyanate, 3 to 8 parts of trimethylolpropane, 10 to 20 parts of methyl methacrylate, 8 to 15 parts of butyl acrylate, 0.5 to 2 parts of benzoyl peroxide and 0.2 to 0.8 part of dibutyltin dilaurate. The invention relates to an anti-aging coating which comprises the following components in parts by weight: 20-30 parts of a silane coupling agent, 1-3 parts of a silane coupling agent KH-550, 0.3-1 part of an anti-aging agent 264, 5-12 parts of dioctyl phthalate and 20-35 parts of a mixed organic solvent. The raw materials are scientifically proportioned, and the cold resistance and tear resistance of the coating are remarkably improved through the synergistic effect of the modified epoxy resin and the modified nano silicon dioxide; water is strictly controlled in the whole process, side reaction is avoided, and stable resin structure is guaranteed; the preparation steps are clear and definite, the repeatability is high, the solvent system adaptability is good, construction is convenient and fast, and the method is suitable for base material protection in various low-temperature scenes.

The invention discloses an endophytic fungus for improving cold resistance of fraxinus mandshurica, a composition and application, and belongs to the technical field of microorganisms, the endophytic fungus is named as Didymellaapomorum fungus FMS1 and preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC NO.42058. The endophytic fungus has the advantages that the endophytic fungus is named as Didymellaapomorum fungus FMS1, and the preservation number is CGMCC NO.42058; according to the method, fraxinus mandshurica is used as a material, root irrigation or spraying treatment is conducted on the fraxinus mandshurica through endophytic fungi FMS1 of the fraxinus mandshurica, the cold resistance of the fraxinus mandshurica can be improved, and the plant height, stem diameter and other characters of the fraxinus mandshurica under low-temperature stress are remarkably improved; according to the technical scheme, the problems that fraxinus mandshurica is not cold-resistant and low in survival rate in the seedling stage can be effectively solved, the ability of the fraxinus mandshurica to deal with abnormal low temperature is improved, the low-temperature area planting area of the fraxinus mandshurica can be widened, and the method is suitable for large-area popularization and has important application prospects.

The invention relates to the technical field of analysis and testing, in particular to a method and system for detecting the cold resistance degree of pennisetum purpureum based on spectral imaging, and the method comprises the steps: obtaining and correcting a spectral image of a pennisetum purpureum leaf; determining biochemical values of various induction components, calculating weights by using principal component analysis, and screening out the component with the maximum weight as a target prediction index; extracting the average spectrum data of the region of interest, and constructing a regression prediction model in combination with a target index measured value after preprocessing and characteristic wave band screening; and finally, the index content is inversed by using the model to represent the cold resistance degree. According to the method, key physiological indexes are scientifically screened through principal component analysis, a high-precision non-destructive detection model is established in combination with an optimal spectrum algorithm, and nondestructive, rapid and high-throughput evaluation of the cold resistance of the object grass is realized.

The invention discloses a fertilizing method for cold resistance and yield increase of heading lettuce, and belongs to the technical field of functional water-soluble fertilizers. The method comprises the following steps: applying a bio-organic fertilizer and a ternary compound fertilizer as a base fertilizer in a planting period; topdressing a water-soluble fertilizer A composed of a liquid nitrogen fertilizer and a cold-resistant functional peptide bacterial fertilizer through drip irrigation in the early rosette stage; topdressing a water-soluble fertilizer B consisting of a high-potassium drip irrigation water flush fertilizer and a cold-resistant functional peptide bacterial fertilizer in the later stage of the rosette stage; topdressing high-potassium drip irrigation and water flush fertilizer at the initial stage of the heading stage. On the basis of a water and fertilizer integrated system, the cold-resistant functional peptide bacterial fertilizer is precisely applied in stages, the liquid nitrogen fertilizer and the high-potassium water-soluble fertilizer are optimized, the synergistic effect of peptide substances, functional microorganisms and nutrient elements is fully played, the cold resistance of the heading lettuce is remarkably enhanced, nitrogen is reduced by 15%-20%, the yield is increased by 40%-50%, the income is increased by 1200-1300 yuan per mu, and the yield is increased by 100-200 yuan per mu. And the method has important popularization and application values.

The invention belongs to the technical field of plant genetic engineering, and particularly discloses application of an important member of a potato mitogen-activated protein kinase (MAPK) family, namely an StMKK9 gene in regulation and control of maturity and cold resistance of potatoes. CDNA of a cultivar Desiree leaf is used as a template, the potato StMKK9 gene is separated and cloned, an over-expression vector is constructed, and the over-expression vector is used for expression of the potato. Genetic transformation of the potatoes is further carried out, it is found that the overexpressed StMKK9 gene can promote prematurity of the potatoes, meanwhile, the cold resistance of potato plants can be improved, and a new genetic material and a theoretical basis are provided for analysis of a potato tuberization molecular mechanism and improved breeding of the potatoes.

The invention aims to provide a model and a method for identifying the cold resistance of alfalfa in the bud stage, the identification model relates to the relationship between four cold-resistant indexes and cold-resistant grade (Y) of alfalfa at 6 + / -0.1 DEG C, the four cold-resistant indexes are alfalfa germination rate (X1), alfalfa simplified vigor index (X2), alfalfa root length (X3) and alfalfa germination index (X4), and the relational expression is Y =-2.771 + 0.016 X1 + 0.061 X2 + 0.900 X3 + 0.054 X4, when Y is more than or equal to 0.70 and less than or equal to 1.00, the alfalfa variety is a strong cold-resistant variety; when Y is more than 0.40 and less than 0.70, the alfalfa variety is a medium cold-resistant variety; when Y is more than or equal to 0.00 and less than or equal to 0.40, the alfalfa variety is a non-cold-resistant variety. Experiments prove that the model has the characteristics of high accuracy, rapidness and high efficiency when being used for identifying alfalfa cold-resistant germplasm resources.

The invention relates to the field of fabric preparation, in particular to a cold-proof and oxygen-resistant fabric applied to extreme working conditions and a preparation method of the cold-proof and oxygen-resistant fabric. The fabric is formed by compounding a honeycomb base body outer layer, a middle buffer layer and a functional inner layer. The outer layer is hexagonal honeycomb grid base cloth formed by weaving basalt filaments and PTFE modified polyimide core-spun yarns, and composite colloid is injected for stepped curing; the middle layer is formed by sewing a silicon dioxide aerogel felt subjected to electrostatic spraying of paraffin microcapsules and a flame-retardant pre-oxidized fiber felt; and the inner layer is an oxygen adsorption layer formed by activating polyimide / flame-retardant viscose blended yarns and then grafting the activated polyimide / flame-retardant viscose blended yarns with a ZIF-8 material. All the layers are separated through polybenzimidazole membranes, and the composite fabric with excellent cold resistance, flame retardance, oxygen resistance and structural stability is finally obtained through gradient hot pressing, water-cooling shaping and surface flame-retardant coating treatment and is suitable for protective equipment in an extreme environment.

The invention discloses a composition for enhancing cold resistance based on pyroptosis regulation and a preparation method thereof, and belongs to the technical field of biological medicines. The preparation method of the composition for enhancing cold resistance based on pyroptosis regulation comprises the following steps: S1, respectively preparing an angelica sinensis aqueous extract and an astragalus membranaceus aqueous extract; and S2, mixing the angelica sinensis aqueous extract and the astragalus membranaceus aqueous extract according to the mass ratio of 1: (4-20) to obtain the composition for enhancing cold resistance based on pyroptosis regulation. The invention further provides a composition obtained based on the preparation method, a preparation and application. The composition provided by the invention can significantly prolong the survival time of acute cold exposure mice; the core body temperature of an acute cold exposure mouse is obviously improved, liver injury is relieved, and liver tissue cell pyroptosis pathway protein expression is regulated.