Microbial fertilizer-plant synbiotic composition preparation and method for preparing symbiotic preparation
A microbial fertilizer and synbiotic technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, fertilizer mixtures, etc., can solve the problems of research and application limited to animals, single function, and increase fertilizer efficiency, so as to overcome the obstacles of continuous cropping , increase soil nutrients, improve the effect of promoting growth
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0069] A microbial fertilizer-plant synbiotic composition preparation. According to weight ratio, the preparation consists of 85 to 99 parts of probiotics, 0.05 to 3.5 parts of prebiotics, and 1.5 to 10 parts of stabilizers to form plant synbiotics, wherein the probiotics The ratio of plant synbiotics with prebiotics ranges from 30 to 60 parts:1 part (W / W).
[0070] A method for preparing a synbiotic preparation is carried out according to the following steps:
[0071] 1) Fermentation of probiotics
[0072] ①. Seed production: connect different strains to different sterile plate culture media, and incubate at a constant temperature of 32℃~37℃ for 48h. On a sterile operating table, use 10~15mL of sterile water to The cells are fully suspended, transferred to sterilized eggplant-shaped bottles, incubated at a constant temperature of 32°C to 37°C for 48 hours, treated at a temperature of 80°C for 10 minutes, and used for fermentation tank inoculation;
[0073] ② Fermentation: Ferment...
Embodiment 2
[0085] A microbial fertilizer-plant synbiotic composition preparation. According to weight ratio, the preparation consists of 90 to 95 parts of probiotics, 0.05 to 2.0 parts of prebiotics, and 3 to 6 parts of stabilizers to form plant synbiotics, wherein the probiotics The ratio of plant synbiotics with prebiotics ranges from 45 to 60 parts:1 part (W / W).
[0086] A method for preparing a synbiotic preparation is carried out according to the following steps:
[0087] 1) Fermentation of probiotics
[0088] ①. Seed production: connect different strains to different sterile plate culture media, culture at a constant temperature of 35°C for 48 hours, and fully suspend the bacteria on the colony on a sterile operating table with 15 mL of sterile water , Transferred to a sterilized eggplant-shaped bottle, incubated at a temperature of 35 ℃ for 48 hours, and treated at a temperature of 80 ℃ for 10 minutes, used for inoculation of the fermenter;
[0089] ② Fermentation: Fermentation medium...
Embodiment 3
[0102] Bacillus subtilis (Bacilus subtis) culture
[0103] Bacillus subtilis slant medium: peptone 10g, beef extract 5g, NaCl 5g, agar 20g, water 1000ml, pH 7.2.
[0104] Bacillus subtilis liquid culture medium: soybean rice 1000g, white sugar 1000g, CaCO3 500g, NaCl 30g, kCl 30g, MgSO4 30g, (NH4)2 SO4 50g, Mn SO4 3g, Fe SO4 3g, water 100L.
[0105] Bacillus subtilis culture characteristics: the maximum growth temperature is 40-50℃, the optimum growth temperature is 33-37℃, the optimum fermentation temperature is 29-33℃, and the optimum pH is 5.5-8.5.
[0106] Morphological characteristics of Bacillus subtilis:
[0107] Gram-positive bacilli, about 0.6-0.8×1.5-3.0 microns in size. Cells grown on glucose agar medium stained evenly and moved. The spores are oval in shape, and the spores are medium or partial, not enlarged or slightly enlarged. The colony is round, the surface is dark, rough, and the edges are irregular.
[0108] The liquid culture conditions of the fermenter: the ch...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com