36results about How to "Enhance growth-promoting effect" patented technology

GLP-2 analogues are disclosed which comprise one of more substitutions as compared to [hGly2]GLP-2 and which improved biological activity in vivo and/or improved chemical stability, e.g., as assessed in in vitro stability assays. More particularly, preferred GLP-2 analogues disclosed herein comprise substitutions at one or more of positions 8, 16, 24 and/or 28 of the wild-type GLP-2 sequence, optionally in combination with further substitutions at position 2 (as mentioned in the introduction) and one or more of positions 3, 5, 7, 10 and 11, and/or a deletion of one or more of amino acids 31 to 33 and/or the addition of a N-terminal or C-terminal stabilizing peptide sequence. The analogues are particularly useful for the prophylaxis or treatment of stomach and bowel-related disorders and for ameliorating side effects of chemotherapy. Also disclosed are methods and kits for selecting a patient from populations suited for treatment with GLP-2 analogues.

The invention discloses a microbial fertilizer-plant synbiotic composition preparation and a method for preparing the synbiotic preparation. In the composition preparation, the plant synbiotic consists of probiotics, prebiotics and stabilizer; and bacillus forms zymotic fluid. The method for preparing the synbiotic preparation comprises the following steps: fermenting the probiotics; regulating bacteria amount; and preparing and homogenizing to obtain the product. Azotobacter, pralidoxime iodide and potassium bacteria in the preparation can increase soil nutrition, improve the fertilizer utilization rate and soil granule structures, promote the air permeability, water-retaining property and drainage, and overcome the defect of sunlight greenhouse continuous cropping; oligosaccharide prebiotics in the preparation can stimulate crops to improve resistance to pathogenic microorganisms, and promotes permanent planting and proliferation of the probiotics; the preparation can inhibit toxicity generated by bacteria, start self immunity system, eliminate diseases and remove evils, reduce bactericidal pesticide, has cold resistance, heat resistance, drought resistance, waterlogging resistance, saline and alkaline resistance, fertilizer damage resistance, chemical damage resistance and nutrition imbalance resistance, can promote plant root elongation and good development of capillary roots, and promote blooming and fruit yielding.

The application relates to the treatment of conditions and diseases for which growth hormone is a desirable method of treatment, using free fatty acid regulators in combination with growth hormone. In particular, the present invention discloses an enhanced method of treatment of growth disorders as well as methods to prevent and/or reduce adverse consequences of growth hormone treatment.

The invention relates to vibrion LX6-2 and application thereof in preparation of a biological seaweed fertilizer. The preparation steps of the biological seaweed fertilizer are as follows: slant cultivation of the vibrion LX6-2, seed cultivation, preparation of seaweed digestive juice, fermentation of the biological seaweed liquid and the like. The content of gibberellins of the biological seaweed fertilizer is not less than 5000ppm; compared with the conventional chemical treatment method and other bio-treatment methods, the content of the gibberellins is improved by 1 to 2 times, so that plant growth development is effectively promoted and the crop yield is increased. The composite biological seaweed fertilizer disclosed by the invention also has a soil improvement effect in promoting soil to form pellets and enhancing soil permeability, has functions of obviously improving drought-resistant and stress-tolerant ability of plants and obviously promoting plant growth, has a good control effect on take-all disease of wheat and bacterial wilt of pepper, and has a good prevention and treatment effect on rhizoctonia cerealis, rhizoctonia solani and the like.

A plant-cultivating method is provided which comprises a red light irradiation step (A) and a blue light irradiation step (B), wherein the step (A) and the step (B) are independently carried out for a predetermined period of time under cultivation conditions such that the concentration of carbon dioxide in a cultivation atmosphere at the step (B) is higher than that at the step (A). Preferably the concentrations of carbon dioxide at the step (B) and the step (A) are 1000-2500 ppm and 700-1500 ppm, respectively. The concentration of carbon dioxide at the step (B) is preferably at least 200 ppm higher than that at the step (A).

The invention provides a method for preparing a fermentation insecticide by taking radix stemonae as a main raw material. The technological characteristics are as follows: radix stemonae is taken as the main raw material, a domestic waste, a starch substance and water are taken as auxiliary materials, the commercially available bacillus pumilus, bacillus thuringiensis and bacillus amyloliquefaciens H47 are fermentation strains, the ratio of radix stemonae to the domestic waste to the starch substance to purified water is (2-5):(1-2):0.01:(3-7), the inoculum size of the strain accounts for 2-5% of the system, the fermentation temperature is 25-35 DEG C, the revolving speed is 100-200 rpm, the pH is kept to 5-8, and the fermentation time is 6-9 days. The raw materials are completely fermented, various enzymes, vitamins, amino acids and microelements are fully released, and the effect and the function of the fermentation insecticide are improved. In addition, the physiological activator of the botanical insecticide is not affected.

The invention provides a plant growth-promoting complex microbial inoculant produced based on ecological relationship among strains and application thereof. The plant growth-promoting complex microbial inoculant is prepared by mixing bacterial liquids of bacillus amyloliquefaciens SQR9, bacillus amyloliquefaciens RF-CU-1, lysine bacillus RF-CU-2 and brevibacillus pulcherrimus RF-CU-3. The bacillus synthetic flora can cooperate with each other and remarkably promote growth of cucumber plants, and under the sterile water culture condition in a laboratory, the growth-promoting effect is remarkably improved along with improvement of diversity of inoculated flora.

The invention discloses an indole acetaldehyde dehydrogenase gene ald2 and an overexpression method and application thereof. An IAA biosynthesis key gene ald2 of Trichoderma harzianum NJAU4742 is disclosed, and the nucleotide sequence is shown in SEQ ID NO: 1. The gene ald2 of IAA biosynthesis key gene ald2 of Trichoderma harzianum NJAU4742 is provided. A method for overexpression of an indoleacetaldehyde dehydrogenase gene ald2. The application of indole aldehyde dehydrogenase gene ald2 and its overexpressed fragment in the construction of Trichoderma harzianum NJAU4742 with significantly increased IAA production. The ald2 gene of the newly discovered Trichoderma harzianum NJAU4742 plays an important role in the IAA synthesis process, and the IAA content at the columnar sheath position inthe root hair region of the plant main root system can be significantly increased after the gene is overexpressed. The present invention provides theoretical basis and technical guarantee for the development of novel Trichoderma bio-organic fertilizer and ecological agriculture.

The invention discloses a cellulose degradation complex microbial inoculant and a preparation method thereof. The cellulose degradation complex microbial inoculant is prepared from bacillus mycoides, serratia marcescens, corynebacterium glutamicum, trichoderma reesei and aspergillus fumigatus. The total viable count of the complex microbial inoculant is greater than or equal to 3.75 * 10 < 8 > cfu/mL. The complex microbial inoculant provided by the invention can improve the production efficiency of the organic fertilizer taking the high-cellulose-content organic waste as the fermentation raw material, promote the resource utilization of the high-cellulose-content organic waste such as straw and phytophagous animal manure, and provide a technical basis for the production of the high-quality organic fertilizer and the development of an agricultural waste green treatment mode.

The invention discloses pseudomonas fluorescens pf27, an application thereof in promoting plant growth and a microbial agent pf27 prepared from the pseudomonas fluorescens pf27. Experiments prove that the microbial agent pf27 can remarkably increase the plant height, the root length and the like of plants after being treated and has a certain growth promoting function on plants; comparison between crops treated with the microbial agent pf27 with a control group indicates that the growth promoting effect of the microbial agent pf27 is higher than 21.55% and can be up to 829.14% to the maximum extent, the microbial agent pf27 has a broad-spectrum and efficient plant growth promoting function and especially can promote growth of solanaceous vegetable type crops and improve the quality of the solanaceous vegetable type crops. The problems that chemical fertilizers and other methods are poor in effect or have residues and the like are solved, agricultural sustainable development is promoted, and pseudomonas fluorescens pf27 and the microbial agent pf27 have better application prospects.

The invention provides a method for preparing a pesticide by fermenting pinellia ternata. The method is technologically characterized in that the pinellia ternata is used as a raw material and is supplemented by household garbage, starchy materials and water, commercially available bacillus pumilus, bacillus thuringiensis and bacillus amyloliquefaciens H47 are used as fermenting strains, the method is based on the proportion of the pinellia ternata, the household garbage, the starchy materials and the purified water 1-3 to 1-2 to 0.01 to 7-8 and the strain inoculation quantity of 1-3%, the fermentation temperature is 25-35 DEG C, the rotating speed is 100-120 rpm, pH is kept to be 5-8, and fermentation time is 6-9 hours. The fermentation is complete, so that various enzymes, vitamins, amino acids and trace elements are fully released to improve the efficacy and function of the pesticide. In addition, the physiologically active substances of plant pesticides are not affected.

The invention discloses a nitrilase gene nit2 and an overexpression method and application thereof. A nitrilase gene nit2, a key gene for IAA biosynthesis in Trichoderma harzianum NJAU4742, has a nucleotide sequence as shown in SEQ ID NO. 1. A method for overexpression of nitrilase gene nit2. The application of nitrilase gene nit2 and its overexpressed fragment in the construction of Trichoderma harzianum NJAU4742 with significantly increased IAA production. The nit2 gene of the newly discovered Trichoderma harzianum NJAU4742 plays an important role in the IAA synthesis process, and the over-expression of the gene can significantly increase the IAA content at the columnar sheath position in the root hair region of the plant main root system. The present invention provides theoretical basisand technical guarantee for the development of novel Trichoderma bio-organic fertilizer and ecological agriculture.

GLP-2 analogues are disclosed which comprise one of more substitutions as compared to [hGly2]GLP-2 and which improved biological activity in vivo and/or improved chemical stability, e.g., as assessed in in vitro stability assays. More particularly, preferred GLP-2 analogues disclosed herein comprise substitutions at one or more of positions 8, 16, 24 and/or 28 of the wild-type GLP-2 sequence, optionally in combination with further substitutions at position 2 (as mentioned in the introduction) and one or more of positions 3, 5, 7, 10 and 11, and/or a deletion of one or more of amino acids 31 to 33 and/or the addition of a N-terminal or C-terminal stabilizing peptide sequence. The analogues are particularly useful for the prophylaxis or treatment of stomach and bowel-related disorders and for ameliorating side effects of chemotherapy. Also disclosed are methods and kits for selecting a patient from populations suited for treatment with GLP-2 analogues.

A plant-cultivating method is provided which comprises a step (A) of irradiating a plant with a red light and a step (B) of irradiating a plant with a blue light, wherein the step (A) and the step (B) are independently carried out for a predetermined period of time under cultivation conditions such that a fertilizer is used for each of the step (A) and the step (B), of which at least the fertilizer used for the step (A) is applied in the form of a nutritious liquid containing fertilizer ingredients and further carbon dioxide added therein. Preferably, a nutritious liquid is applied at each of the step (A) and the step (B), and the nutritious liquid applied at the step (A) contains carbon dioxide at a concentration higher than that in the nutritious liquid applied at the step (B).

The invention belongs to the field of microorganisms, and in particular relates to a high-yielding γ-PGA bacillus subtilis and its application. The main technical scheme is as follows: a new strain of Bacillus subtilis is provided, the preservation number is: CGMCC NO.17215; its 16Sr DNA sequence is shown in SEQ ID NO 1. And based on the bacillus subtilis, an application method for improving soil environment and promoting plant growth is provided. Using the Bacillus subtilis provided by the present invention and its application method, high-yield γ-PGA can be obtained at a lower temperature, which greatly saves production energy consumption; effectively improves the growth performance of plants and reduces the absorption of heavy metals in soil by plants .