36results about How to "Enhance growth-promoting effect" patented technology

GLP-2 analogues are disclosed which comprise one of more substitutions as compared to [hGly2]GLP-2 and which improved biological activity in vivo and / or improved chemical stability, e.g., as assessed in in vitro stability assays. More particularly, preferred GLP-2 analogues disclosed herein comprise substitutions at one or more of positions 8, 16, 24 and / or 28 of the wild-type GLP-2 sequence, optionally in combination with further substitutions at position 2 (as mentioned in the introduction) and one or more of positions 3, 5, 7, 10 and 11, and / or a deletion of one or more of amino acids 31 to 33 and / or the addition of a N-terminal or C-terminal stabilizing peptide sequence. The analogues are particularly useful for the prophylaxis or treatment of stomach and bowel-related disorders and for ameliorating side effects of chemotherapy. Also disclosed are methods and kits for selecting a patient from populations suited for treatment with GLP-2 analogues.

The invention discloses a microbial fertilizer-plant synbiotic composition preparation and a method for preparing the synbiotic preparation. In the composition preparation, the plant synbiotic consists of probiotics, prebiotics and stabilizer; and bacillus forms zymotic fluid. The method for preparing the synbiotic preparation comprises the following steps: fermenting the probiotics; regulating bacteria amount; and preparing and homogenizing to obtain the product. Azotobacter, pralidoxime iodide and potassium bacteria in the preparation can increase soil nutrition, improve the fertilizer utilization rate and soil granule structures, promote the air permeability, water-retaining property and drainage, and overcome the defect of sunlight greenhouse continuous cropping; oligosaccharide prebiotics in the preparation can stimulate crops to improve resistance to pathogenic microorganisms, and promotes permanent planting and proliferation of the probiotics; the preparation can inhibit toxicity generated by bacteria, start self immunity system, eliminate diseases and remove evils, reduce bactericidal pesticide, has cold resistance, heat resistance, drought resistance, waterlogging resistance, saline and alkaline resistance, fertilizer damage resistance, chemical damage resistance and nutrition imbalance resistance, can promote plant root elongation and good development of capillary roots, and promote blooming and fruit yielding.

The application relates to the treatment of conditions and diseases for which growth hormone is a desirable method of treatment, using free fatty acid regulators in combination with growth hormone. In particular, the present invention discloses an enhanced method of treatment of growth disorders as well as methods to prevent and / or reduce adverse consequences of growth hormone treatment.

GLP-2 analogues are disclosed which comprise one of more substitutions as compared to [hGly2]GLP-2 and which improved biological activity in vivo and / or improved chemical stability, e.g. as assessed in in vitro stability assays. More particularly, preferred GLP-2 analogues disclosed herein comprise substitutions at one or more of positions 8, 16, 24 and / or 28 of the wild-type GLP-2 sequence, optionally in combination with further substitutions at position 2 (as mentioned in the introduction) and one or more of positions 3, 5, 7, 10 and 11, and / or a deletion of one or more of amino acids 31 to 33 and / or the addition of a N-terminal or C-terminal stabilizing peptide sequence. The analogues are particularly useful for the prophylaxis or treatment of stomach and bowel-related disorders and for ameliorating side effects of chemotherapy.

The invention relates to vibrion LX6-2 and application thereof in preparation of a biological seaweed fertilizer. The preparation steps of the biological seaweed fertilizer are as follows: slant cultivation of the vibrion LX6-2, seed cultivation, preparation of seaweed digestive juice, fermentation of the biological seaweed liquid and the like. The content of gibberellins of the biological seaweed fertilizer is not less than 5000ppm; compared with the conventional chemical treatment method and other bio-treatment methods, the content of the gibberellins is improved by 1 to 2 times, so that plant growth development is effectively promoted and the crop yield is increased. The composite biological seaweed fertilizer disclosed by the invention also has a soil improvement effect in promoting soil to form pellets and enhancing soil permeability, has functions of obviously improving drought-resistant and stress-tolerant ability of plants and obviously promoting plant growth, has a good control effect on take-all disease of wheat and bacterial wilt of pepper, and has a good prevention and treatment effect on rhizoctonia cerealis, rhizoctonia solani and the like.

The invention discloses phosphorus-solubilizing growth-promoting bacteria Arthrobacter pascens X-1 for promoting growth of root nodules and improving the population abundance of probiotic microorganisms, and belongs to the technical field of microorganisms. The Arthrobacter pascens X-1 promotes production of soybean plants, promotes proliferation of the root nodules of the plants, and improves theprobiotic microorganisms and the nutritional environment. After the Arthrobacter pascens X-1 is applied, nutrient elements such as potassium, calcium and magnesium required by the plants in rock powder are effectively released, rock erosion soil formation is accelerated, meanwhile, effective nitrogen fixation of soybeans is promoted, nitrogen required by growth of the soybeans is provided, the symbiotic nitrogen fixation capacity of rhizobium and host plants is obviously exerted, the total weight of the root nodules is increased by 150.00%, the content of hydrolyzed nitrogen is increased by 11.58%, and the symbiotic nitrogen fixation capacity is effectively exerted. Meanwhile, the relative abundance of Bradyrhizobium in soil is increased from 0.21% to 52.47%, and the number of the increased relative abundance of the Bradyrhizobium and the promoting effect on plant bodies are superior to the effect of direct application of the Bradyrhizobium.

A plant-cultivating method is provided which comprises a red light irradiation step (A) and a blue light irradiation step (B), wherein the step (A) and the step (B) are independently carried out for a predetermined period of time under cultivation conditions such that the concentration of carbon dioxide in a cultivation atmosphere at the step (B) is higher than that at the step (A). Preferably the concentrations of carbon dioxide at the step (B) and the step (A) are 1000-2500 ppm and 700-1500 ppm, respectively. The concentration of carbon dioxide at the step (B) is preferably at least 200 ppm higher than that at the step (A).

The invention discloses a microbial source combined biostimulant. The microbial source combined biostimulant is characterized by being prepared from the following components, by mass: 0.1 to 17.5 percent of polyglutamic acid, 0 to 5.0 percent of amino-oligosaccharin, 0.05 to 2.0 percent of pantoea polysaccharide, 0 to 1.5 percent of gamma-aminobutyric acid and the balance of auxiliary materials. The microbial source combined biostimulant can effectively improve the utilization rate of a plant fertilizer, improve the disease resistance and stress resistance of the plant fertilizer and induce aself-defense system of the plant fertilizer, can be completely biodegraded in soil, and is non-toxic, harmless and pollution-free to plants and the soil environment.

The invention provides a method for preparing a fermentation insecticide by taking radix stemonae as a main raw material. The technological characteristics are as follows: radix stemonae is taken as the main raw material, a domestic waste, a starch substance and water are taken as auxiliary materials, the commercially available bacillus pumilus, bacillus thuringiensis and bacillus amyloliquefaciens H47 are fermentation strains, the ratio of radix stemonae to the domestic waste to the starch substance to purified water is (2-5):(1-2):0.01:(3-7), the inoculum size of the strain accounts for 2-5% of the system, the fermentation temperature is 25-35 DEG C, the revolving speed is 100-200 rpm, the pH is kept to 5-8, and the fermentation time is 6-9 days. The raw materials are completely fermented, various enzymes, vitamins, amino acids and microelements are fully released, and the effect and the function of the fermentation insecticide are improved. In addition, the physiological activator of the botanical insecticide is not affected.

The invention provides a plant growth-promoting complex microbial inoculant produced based on ecological relationship among strains and application thereof. The plant growth-promoting complex microbial inoculant is prepared by mixing bacterial liquids of bacillus amyloliquefaciens SQR9, bacillus amyloliquefaciens RF-CU-1, lysine bacillus RF-CU-2 and brevibacillus pulcherrimus RF-CU-3. The bacillus synthetic flora can cooperate with each other and remarkably promote growth of cucumber plants, and under the sterile water culture condition in a laboratory, the growth-promoting effect is remarkably improved along with improvement of diversity of inoculated flora.

The invention discloses an indole acetaldehyde dehydrogenase gene ald2 and an overexpression method and application thereof. An IAA biosynthesis key gene ald2 of Trichoderma harzianum NJAU4742 is disclosed, and the nucleotide sequence is shown in SEQ ID NO: 1. The gene ald2 of IAA biosynthesis key gene ald2 of Trichoderma harzianum NJAU4742 is provided. A method for overexpression of an indoleacetaldehyde dehydrogenase gene ald2. The application of indole aldehyde dehydrogenase gene ald2 and its overexpressed fragment in the construction of Trichoderma harzianum NJAU4742 with significantly increased IAA production. The ald2 gene of the newly discovered Trichoderma harzianum NJAU4742 plays an important role in the IAA synthesis process, and the IAA content at the columnar sheath position inthe root hair region of the plant main root system can be significantly increased after the gene is overexpressed. The present invention provides theoretical basis and technical guarantee for the development of novel Trichoderma bio-organic fertilizer and ecological agriculture.

The invention discloses a cellulose degradation complex microbial inoculant and a preparation method thereof. The cellulose degradation complex microbial inoculant is prepared from bacillus mycoides, serratia marcescens, corynebacterium glutamicum, trichoderma reesei and aspergillus fumigatus. The total viable count of the complex microbial inoculant is greater than or equal to 3.75 * 10 < 8 > cfu / mL. The complex microbial inoculant provided by the invention can improve the production efficiency of the organic fertilizer taking the high-cellulose-content organic waste as the fermentation raw material, promote the resource utilization of the high-cellulose-content organic waste such as straw and phytophagous animal manure, and provide a technical basis for the production of the high-quality organic fertilizer and the development of an agricultural waste green treatment mode.

The invention discloses pseudomonas fluorescens pf27, an application thereof in promoting plant growth and a microbial agent pf27 prepared from the pseudomonas fluorescens pf27. Experiments prove that the microbial agent pf27 can remarkably increase the plant height, the root length and the like of plants after being treated and has a certain growth promoting function on plants; comparison between crops treated with the microbial agent pf27 with a control group indicates that the growth promoting effect of the microbial agent pf27 is higher than 21.55% and can be up to 829.14% to the maximum extent, the microbial agent pf27 has a broad-spectrum and efficient plant growth promoting function and especially can promote growth of solanaceous vegetable type crops and improve the quality of the solanaceous vegetable type crops. The problems that chemical fertilizers and other methods are poor in effect or have residues and the like are solved, agricultural sustainable development is promoted, and pseudomonas fluorescens pf27 and the microbial agent pf27 have better application prospects.

The invention provides a method for preparing a pesticide by fermenting pinellia ternata. The method is technologically characterized in that the pinellia ternata is used as a raw material and is supplemented by household garbage, starchy materials and water, commercially available bacillus pumilus, bacillus thuringiensis and bacillus amyloliquefaciens H47 are used as fermenting strains, the method is based on the proportion of the pinellia ternata, the household garbage, the starchy materials and the purified water 1-3 to 1-2 to 0.01 to 7-8 and the strain inoculation quantity of 1-3%, the fermentation temperature is 25-35 DEG C, the rotating speed is 100-120 rpm, pH is kept to be 5-8, and fermentation time is 6-9 hours. The fermentation is complete, so that various enzymes, vitamins, amino acids and trace elements are fully released to improve the efficacy and function of the pesticide. In addition, the physiologically active substances of plant pesticides are not affected.

The invention discloses a preparation of compound rice dedicated bio-fertilizer based on arbuscular mycorrhizal fungi and an application thereof, and relates to the preparation and the application of bio-fertilizer. The compound rice dedicated bio-fertilizer aims to solve the problem that rice can not be infected by the arbuscular mycorrhizal fungi under the aquatic condition at present and is difficult to effectively form mutualistic symbiosis, and therefore the rice can not obtain mineral nutrition from an arbuscular mycorrhizal fungi-crop symbiotic system. The method comprises the following steps of: preparing a colonized reinforcer; pre-processing arbuscular mycorrhizal fungi fungicide; and evenly mixing the processed arbuscular mycorrhizal fungi fungicide, rice shoots growth promoter and the colonized reinforce. The compound rice dedicated bio-fertilizer can be prepared into a seedling bed, culturing the mycorrhiza seedling and managing a rice field. According to the compound rice dedicated bio-fertilizer disclosed by the invention, the arbuscular mycorrhizal fungi-crop symbiotic system with a high infection rate and stable performance can be built in the seedling stage of the rice, and the symbiotic system can successfully live through the transplanted field flooding environment. The biological bactericide activity loss problem brought by flooding intimidation can be effectively solved, mineral nutrition is continuously provided for the rice, the growth situation of the rice is improved, the fertilizer application amount in the field is reduced, and the rice production cost can be effectively lowered.

The invention provides a functional microbial agent for preventing and treating root-knot nematodes, which comprises nitrogen-fixing bacterial agent, biocontrol bacterial agent and carrier, and the mass ratio of the nitrogen-fixing bacterial agent, biocontrol bacterial agent and carrier is 1 to 2.5 : 1.5~5:1, the nitrogen-fixing bacterial agent comprises rhizobia and Azotobacter torus, the ratio of the effective number of viable bacteria of the rhizobia to Azotobacter torus is 1~100:1~200, and the biocontrol Bacteria agent comprises Paecilomyces lilacinus, Verticillium chlamydoides, Bacillus subtilis, Bacillus cereus, the effective activity of said Paecilomyces lilacinus, Verticillium chlamydoides, Bacillus subtilis, Bacillus cereus The bacterial count ratio is 1-100:1-100:1-100:1-100, and the effective viable bacterial count of the functional bacterial agent is greater than 200 million / g. The functional bacterial agent in the present invention is applied to fertilizers, which can increase the disease prevention effect of microbial fertilizers, improve its growth-promoting effect, ensure the nutritional supply of microbial fertilizers in the application process, prevent and control root-knot nematodes, and form healthy root nodules. Promote plant growth, improve crop yield and quality.

The invention discloses a nitrilase gene nit2 and an overexpression method and application thereof. A nitrilase gene nit2, a key gene for IAA biosynthesis in Trichoderma harzianum NJAU4742, has a nucleotide sequence as shown in SEQ ID NO. 1. A method for overexpression of nitrilase gene nit2. The application of nitrilase gene nit2 and its overexpressed fragment in the construction of Trichoderma harzianum NJAU4742 with significantly increased IAA production. The nit2 gene of the newly discovered Trichoderma harzianum NJAU4742 plays an important role in the IAA synthesis process, and the over-expression of the gene can significantly increase the IAA content at the columnar sheath position in the root hair region of the plant main root system. The present invention provides theoretical basisand technical guarantee for the development of novel Trichoderma bio-organic fertilizer and ecological agriculture.

The invention discloses a waste-free and full-utilization method for preparing a foliar fertilizer by using a phosphorus-potassium method, comprising the following steps: (1) extracting chitin; (2) preparing chitosan; (3) preparing chitosan oligosaccharide by using chitosan; (4) using a waste liquid mixture for microbial fermentation of soybean meal, and extracting a fermentation product after fermentation; and (5) mixing the fermentation product with the chitosan solution, and chelating the mixture with trace meter to obtain a foliar fertilizer. According to the method, by recovering the waste liquid in the process of preparing chitosan oligosaccharide from shrimp shell and mixing with soybean meal for fermenting, the content of the fermentation product NPK can be increased; and the fermentation product is compounded with chitosan oligosaccharide and trace metal to obtain the foliar fertilizer, which can improve the plant growth-promoting effect. The method is simple to operate, no waste liquid, waste gas or waste solid is discharged, and full utilization of shrimp shell is realized.

The invention provides a compound microbial agent suitable for saline-alkali soil in arid and semi-arid regions and application of the compound microbial agent. The compound microbial agent disclosed by the invention comprises alfalfa rhizobium, functional water-retaining peptide and drought-enduring bacteria, wherein the drought-tolerant bacteria are endophytic bacillus flexus CGMCC (China General Microbiological Culture Collection Center) No.21705, and the functional water retention peptide is prepared by fermenting bacillus licheniformis CGMCC No.8821. The compound microbial agent disclosed by the invention can be used for improving the activity of crop root systems and the drought resistance and saline-alkali resistance of crops, is particularly suitable for crop planting in arid and semi-arid climate regions, obviously improves the nitrogen fixation performance of crops under arid conditions, obviously improves the adaptive capacity of crop root systems under water drought and saline-alkali stress, improves the stress resistance of crops, and improves the yield of crops. The purposes of improving the soil performance and increasing the yield are achieved, and the method has important significance on improvement of the ecological planting environment of saline-alkali soil in arid and semi-arid regions and sustainable development of modern agriculture.

GLP-2 analogues are disclosed which comprise one of more substitutions as compared to [hGly2]GLP-2 and which improved biological activity in vivo and / or improved chemical stability, e.g., as assessed in in vitro stability assays. More particularly, preferred GLP-2 analogues disclosed herein comprise substitutions at one or more of positions 8, 16, 24 and / or 28 of the wild-type GLP-2 sequence, optionally in combination with further substitutions at position 2 (as mentioned in the introduction) and one or more of positions 3, 5, 7, 10 and 11, and / or a deletion of one or more of amino acids 31 to 33 and / or the addition of a N-terminal or C-terminal stabilizing peptide sequence. The analogues are particularly useful for the prophylaxis or treatment of stomach and bowel-related disorders and for ameliorating side effects of chemotherapy. Also disclosed are methods and kits for selecting a patient from populations suited for treatment with GLP-2 analogues.

A plant-cultivating method is provided which comprises a step (A) of irradiating a plant with a red light and a step (B) of irradiating a plant with a blue light, wherein the step (A) and the step (B) are independently carried out for a predetermined period of time under cultivation conditions such that a fertilizer is used for each of the step (A) and the step (B), of which at least the fertilizer used for the step (A) is applied in the form of a nutritious liquid containing fertilizer ingredients and further carbon dioxide added therein. Preferably, a nutritious liquid is applied at each of the step (A) and the step (B), and the nutritious liquid applied at the step (A) contains carbon dioxide at a concentration higher than that in the nutritious liquid applied at the step (B).

The invention belongs to the field of microorganisms, and in particular relates to a high-yielding γ-PGA bacillus subtilis and its application. The main technical scheme is as follows: a new strain of Bacillus subtilis is provided, the preservation number is: CGMCC NO.17215; its 16Sr DNA sequence is shown in SEQ ID NO 1. And based on the bacillus subtilis, an application method for improving soil environment and promoting plant growth is provided. Using the Bacillus subtilis provided by the present invention and its application method, high-yield γ-PGA can be obtained at a lower temperature, which greatly saves production energy consumption; effectively improves the growth performance of plants and reduces the absorption of heavy metals in soil by plants .

The invention discloses a method for producing protease for promoting growth of plant roots and fruits. The method comprises the following steps: S1, collecting stems and leaves of the same plant or plants in the same genus, namely selecting stem leaves, tubers and root-tubers of the plant in the same family or same genus, and performing collection; S2, performing pretreatment, namely washing thecollected stem leaves, tubers or root-tubers, and performing grinding treatment; S3, adding a phosphate buffer into the ground material, namely performing stirring and homogenizing, adding a phosphatebuffer into the ground material, performing sufficient stirring and uniform homogenizing; S4, performing filtering and centrifuging to obtain supernate and residues, namely filtering the homogenate,and centrifuging the filtrate to obtain the supernate and residues; and S5, performing ammonium sulfate precipitation on the supernate, namely centrifugally collecting upper insoluble substances, andadding ammonium sulfate into the supernate to perform salt precipitation. In the method, stem leaves, tubers and root-tubers of the plant in the same family or the same genus are collected to produceprotease, so that the effect of a produced finished product on promoting growth of roots and fruits of the plant can be greatly promoted along with good adaptability and small side effect.

The invention discloses a nitrilase gene nit2 and its overexpression method and application. A key gene for IAA biosynthesis in Trichoderma harzianum NJAU4742 is a nitrilase gene nit2, the nucleotide sequence of which is shown in SEQ ID NO.1. A method for overexpression containing the nitrilase gene nit2. Application of nitrilase gene nit2 and its overexpression fragment in constructing Trichoderma harzianum NJAU4742 genetically engineered bacteria with significantly improved IAA yield. The newly discovered nit2 gene in the Trichoderma harzianum NJAU4742 of the present invention plays an important role in the IAA synthesis process, and the overexpression of the gene can significantly increase the IAA content at the pericycle in the root hair region of the main root system of the plant. The invention provides a theoretical basis and technical support for the development of novel trichoderma bio-organic fertilizer and the development of ecological agriculture.

The invention relates to a Vibrio LX6‑2 and its application in preparing biological seaweed fertilizer. The preparation step of the biological seaweed fertilizer includes the steps of Vibrio LX6‑2 slant culture, seed cultivation, preparation of seaweed digestive juice and fermentation of the biological seaweed liquid. The gibberellin content of the biological seaweed fertilizer of the present invention is more than 5000ppm, which is 1-2 times higher than conventional chemical treatment methods and other biological methods, and can effectively promote plant growth and increase crop yield. The composite biological seaweed fertilizer of the present invention also has the effect of promoting the formation of aggregate structure in the soil, enhancing the soil permeability, and has the function of obviously improving the drought resistance and stress resistance of plants, and has a remarkable effect of promoting growth. It has a good control effect on Rhizoctonia graminearum and Rhizoctonia solani and so on.

The invention discloses an indole acetaldehyde dehydrogenase gene ald2 and its overexpression method and application. A key gene for IAA biosynthesis in Trichoderma harzianum NJAU4742 is an indole acetaldehyde dehydrogenase gene ald2, the nucleotide sequence of which is shown in SEQ ID NO.1. A method for overexpressing the gene ald2 containing indole acetaldehyde dehydrogenase. Application of the indole acetaldehyde dehydrogenase gene ald2 and its overexpressed fragment in the construction of Trichoderma harzianum NJAU4742 genetically engineered bacteria with significantly improved IAA production. The newly discovered ald2 gene in the Trichoderma harzianum NJAU4742 of the present invention plays an important role in the IAA synthesis process, and the overexpression of the gene can significantly increase the IAA content in the pericycle position of the root hair region of the main root system of the plant. The invention provides a theoretical basis and technical support for the development of novel trichoderma bio-organic fertilizer and the development of ecological agriculture.

The invention discloses a strain of Pseudomonas fluorescens pf27 and its application in plant growth promotion. The invention provides a strain of Pseudomonas fluorescens pf27, and prepares a microbial agent pf27. Experiments have shown that the plant height and root length of plants are significantly improved after the microbial agent pf27 treatment, and it has a certain growth-promoting effect on the plants. Up to 829.14%, it has a broad-spectrum and high-efficiency role in promoting plant growth, especially can promote the growth of Solanaceae vegetable crops and improve their quality. The invention solves the problems of poor effect or residues of other methods such as chemical fertilizers, and helps to promote the sustainable development of agriculture, and has a good application prospect.

GLP-2 analogues are disclosed which comprise one of more substitutions as compared to [hGly2]GLP-2 and which improved biological activity in vivo and / or improved chemical stability, e.g., as assessed in in vitro stability assays. More particularly, preferred GLP-2 analogues disclosed herein comprise substitutions at one or more of positions 8, 16, 24 and / or 28 of the wild-type GLP-2 sequence, optionally in combination with further substitutions at position 2 (as mentioned in the introduction) and one or more of positions 3, 5, 7, 10 and 11, and / or a deletion of one or more of amino acids 31 to 33 and / or the addition of a N-terminal or C-terminal stabilizing peptide sequence. The analogues are particularly useful for the prophylaxis or treatment of stomach and bowel-related disorders and for ameliorating side effects of chemotherapy. Also disclosed are methods and kits for selecting a patient from populations suited for treatment with GLP-2 analogues.