13results about How to "Stable growth" patented technology

The application provides a method for inducing poly-podophylli rhizoma by using clump buds, comprising the following steps: S1, cutting stem segments from a podophylli rhizoma mother plant, soaking and cleaning to obtain explants; S2, disinfecting and cleaning the explants, inoculating into a clump bud induction medium to obtain podophylli rhizoma clump buds; S3, inoculating the clump buds into doubling liquid I, washing with a liquid clump bud induction medium after culture, and then transferring into doubling liquid II; S4, taking out the clump buds, cleaning, inoculating into a clump bud induction medium, and then inoculating into a bud proliferation medium; S5, waiting until 1-2 cm, screening out potential mutant strains through phenotypes, inoculating into the bud proliferation medium for subculture, and obtaining mutant plants; and S6, rooting culture of the mutant plants, and after root systems grow, planting and raising seedlings, and then poly-podophylli rhizoma plants are obtained. In the research, suitable concentration of colchicine is used for induction treatment twice, and through stage concentration switching and intermittent recovery strategies, the poly-ploid induction efficiency and explant survival are effectively considered.

The invention relates to a grouper crossbreeding method. The method comprises the four steps of parent breeding, temperature-sensitive domestication, artificial spawning and insemination, incubation and fry breeding, specifically, cold water-based clear water stone spots and hot water-based brown spot stone spots are selected as parents, the clear water stone spots are used as female parents, the brown spot stone spots are used as male parents, and the middle water temperature is adapted through gradient temperature-sensitive domestication; stress-resistant feed containing hilsa herring egg source peptide and haematococcus pluvialis astaxanthin ester and a temperature-sensitive blender are matched, a reproductive care agent is supplemented to strengthen sperm and egg performance, and normative spawning induction, insemination and graded cultivation processes are adopted. According to the method, the problems of poor water temperature adaptation and low hatching rate of grouper hybrid sperms and eggs in different temperature areas are solved, the wide-temperature property of filial generations is improved, the method adapts to the water temperature of 18-28 DEG C, the hatching rate, the fry survival rate, the adult fish survival rate and the average weight of single fish are remarkably improved, and technical support is provided for industrial application.

The invention discloses a methylamine formamidino perovskite single crystal, a preparation method thereof and an X-ray detector, and belongs to the technical field of perovskite single crystal X-ray detectors. Methyl iodide amine, formamidino hydriodate and lead iodide are used as raw materials, anhydrous acetic acid is used as an additive, growth is carried out in a solvent through a seed crystal method, and the methylamine formamidino perovskite single crystal is obtained. Anhydrous acetic acid and formamidine generate hydrogen-bond interaction, formamidine is pulled to enter crystal lattices, so that phase separation is eliminated, acetate and Pb coordinate to delay formation of a lead-iodine framework, the speed of methylamine and formamidine entering the crystal lattices is balanced, meanwhile, the stability of a methylamine formamidine perovskite precursor solution is improved, stable growth of methylamine formamidine perovskite single crystals is achieved, and the methylamine formamidine perovskite single crystals are obtained. The single crystal has the advantages of high crystallinity, low defect density, large size and favorable X-ray response performance. The methylamine formamidine perovskite single crystal is high in controllability in the growth process, and the preparation method is high in operability, simple and easy to implement, short in preparation period, low in equipment requirement and low in cost.

This invention provides a method for preparing a complete-element organic water-soluble fertilizer from traditional Chinese medicine extracts, belonging to the field of fertilizer technology. The raw materials for preparing the complete-element organic water-soluble fertilizer, in addition to the traditional Chinese medicine extract, include molasses fermentation products, macro-elements, micro-elements, adjuvants, and ethylenediaminetetraacetic acid (EDTA). The addition of the traditional Chinese medicine extract enables the complete-element organic water-soluble fertilizer to achieve high quality and high yield when applied, resulting in higher crop yields and stronger disease resistance, overcoming the shortcomings of existing organic water-soluble fertilizers in terms of quality and yield. The traditional Chinese medicine extract is the key raw material, and its preparation involves extracting active ingredients from powders of Erythrina variegata leaves, Atractylodes macrocephala, Cnidium monnieri, and Centella asiatica. The preparation process incorporates enzymatic hydrolysis using cellulase and pectinase. Furthermore, this invention adjusts the timing of the traditional Chinese medicine additions and the extraction temperature to enhance the high-quality and high-yield effects.

This invention discloses a strain of Maggi-Megaki yeast with amine-reducing and flavor-enhancing capabilities and its applications, belonging to the field of microbial fermentation technology. This invention screened a strain of Maggi-Megaki yeast Y21 from fermented soybean paste, which has the ability to degrade biogenic amines. This strain can completely degrade tryptamine, phenylethylamine, putrescine, cadaverine, spermidine, and spermine; simultaneously, it also has a strong degradation effect on histamine and tyramine, with degradation rates reaching 69.58% and 65.32%, respectively. Using the Maggi-Megaki yeast Y21 described in this invention for inoculation and fermentation can significantly reduce the content of various biogenic amines in fermented soybean paste under low-to-medium salt conditions, especially showing outstanding removal effects on histamine, tyramine, phenylethylamine, and polyamine biogenic amines, increasing the total amount of volatile flavor compounds, and is suitable for the safe production of low-salt fermented foods.

This invention provides a method for treating high-salinity wastewater using *Chaetoceros muelleri*, belonging to the field of water treatment: *Chaetoceros muelleri* strains in a preservation state are transferred to a nutritionally complete and suitable standard f / 2 culture medium for activation culture under standard f / 2 conditions; actual marine aquaculture wastewater is used as the treatment target, and after filtering out impurities, the salinity of the wastewater is adjusted to 30-35‰; the *Chaetoceros muelleri* treated in step S1 is inoculated into the high-salinity wastewater at 0.05–0.2 g / L (dry weight), and the lighting system is activated after inoculation; during system operation, the algal biomass and changes in ammonia nitrogen, total nitrogen, and phosphate in the water are monitored regularly; when the pollutant removal rate remains stable at a high level and the algal biomass continues to increase, the system can be considered to be operating stably. This invention efficiently removes nitrogen, phosphorus, and other pollutants from wastewater using *Chaetoceros muelleri* technology, while maintaining stable algal growth, thus improving wastewater treatment efficiency and resource utilization rate.

The application discloses a kind of semiconductor silicon single crystal growth process V / G value prediction and quality evaluation method, belong to the technical field of preparation and intelligent process control of semiconductor material;Including the following steps: obtaining process parameter time series data;Its fragment division is generated continuous data unit, and time series dependence modeling is generated time series characteristic sequence;While it is carried out multi-scale decomposition and obtains high-frequency and low-frequency component, corresponding frequency domain characteristic sequence is generated by fragment division;Time series and frequency domain characteristic sequence are spliced into time-frequency fusion characteristic sequence;Based on the sequence, cross-domain correlation coding and prediction mapping are carried out, and V / G value prediction sequence is output;Finally, based on the prediction sequence, trend category and process quality grade are determined;The application realizes the high-precision trend prediction of V / G value and the intelligent online evaluation of process quality.

This utility model discloses a heat preservation device for flower cultivation, including a heat preservation box. The heat preservation box includes a storage cavity and a water cavity. Water is placed inside the water cavity, and electric heating plates are fixedly connected to both sides of the inner wall of the water cavity. This utility model adopts a structural design in which the heat preservation box has a storage cavity and a water cavity. The water in the water cavity is heated by the electric heating plates. Through the thermal conductivity of water, heat can be evenly transferred to the left, right, top, bottom, and back of the storage cavity, which to a certain extent avoids the problem of uneven local temperature caused by traditional single biological lamp heating. With the heat preservation box and the heat preservation cover plate fixedly connected to the surface of the heat preservation box, a multi-layer heat preservation structure is formed, which can significantly slow down the transfer rate of internal heat to the outside. This device has the advantages of good heat preservation effect, maintaining a suitable internal temperature for a long time, and flexible adjustment of the internal temperature of the device.

This invention provides exosomes derived from rice, their preparation method, and applications. The exosomes have a bilayered membrane spherical structure with a particle size ranging from 20 to 1000 nm. These exosomes are obtained from rice callus tissue and possess high biocompatibility, stability, and sustainable mass production characteristics. Furthermore, experiments have demonstrated that these exosomes have anti-aging, cell regeneration and tissue repair promotion, and protective functions, and can also significantly improve cell absorption efficiency and the delivery efficiency of active ingredients such as peptides.

The application relates to a large-size yttrium lithium fluoride single crystal growth system and process, and belongs to the technical field of artificial crystal growth. The system comprises a furnace body, a crucible, a temperature field subsystem, a vacuum subsystem, a mechanical transmission subsystem, an automatic growth control subsystem and a main control subsystem. The single crystal growth system provided by the application is cooperatively operated by the temperature field subsystem, the vacuum subsystem, the mechanical transmission subsystem and the automatic growth control subsystem under the unified coordination of the main control subsystem, so as to jointly create a crystal growth environment which can be accurately controlled and is stable and reliable. Through a pretreatment process comprising raw material pre-melting, cleaning and weighing and supplementing of raw materials, and in combination with the variable-speed rotation technology of the crucible in the growth process, the purity and the component uniformity of the melt are significantly improved.

This invention discloses an adsorbent based on MOF materials and its processing technology, belonging to the field of adsorbent technology. The process includes the following steps: adding zirconium-based MOF-Fe3O4 to DMF, ultrasonically mixing, adding terephthalaldehyde, mixing, adding acetic acid, reacting at room temperature for 48-72 h, magnetically separating the precipitate, washing, and vacuum drying to obtain aldehyde-based zirconium-based MOF-Fe3O4; placing chromium nitrate nonahydrate and terephthalic acid in DMF, stirring and mixing, adding modified SBA-15, reacting at 180-220℃ for 12-24 h, cooling and filtering, washing and drying to obtain chromium-based MOF-SBA-15; adding aldehyde-based zirconium-based MOF-Fe3O4 and chromium-based MOF-SBA-15 to ethanol, adding acetic acid, reacting at room temperature, magnetically separating, washing and drying to obtain the MOF-based adsorbent. The MOF-based adsorbent prepared by this invention can be applied to the concentration and treatment of typical industrial VOCs waste gas.

The application discloses an immunized tumor organoid and a construction method thereof, and belongs to the technical field of tumor organoids, and the construction method is as follows: under light-proof conditions, a HAMA glue solution containing a photoinitiator is configured, the photoinitiator is added, and mixing is performed to obtain a shell solution; a substrate glue, a culture medium and the HAMA glue are mixed, tumor cells are added thereto, and mixing is performed to obtain a core solution; the shell solution, the core solution and biological oil are respectively injected into a printing chip through a pipeline and discharged through the pipeline, and meanwhile, blue light irradiation is performed to initiate curing, and a double-layer microsphere is obtained; the double-layer microsphere is collected, resuspended and placed in a tumor organoid culture medium for culture, and an immunized tumor organoid is obtained. The method can batch construct tumor organoids under the condition of the same amount of patient-derived tumors, the constructed tumor organoids can simulate a gradual immunization infiltration process of tumor cells and immune cells in the body which is controlled by spatial separation, and the method effectively solves the problem that an immune microenvironment is difficult to reproduce in the construction process of the existing tumor organoids.

This invention discloses a layered manganese dioxide-coated gold nanoparticle photocatalyst and its preparation method, belonging to the field of photocatalytic nanomaterials technology. The preparation method is as follows: S1, tetrachloroauric acid and a quaternary ammonium salt are added to a sodium borohydride solution and stirred at 25-120℃ for 1-3 hours to obtain solution A; solution B containing the quaternary ammonium salt, tetrachloroauric acid, and ascorbic acid is prepared; solutions A and B are mixed and stirred at 25-50℃ for 1-15 hours to obtain Au nanoparticles; S2, after centrifuging the Au nanoparticle dispersion to remove the supernatant, it is added to solution C, which is composed of methoxy polyethylene glycol carboxyl groups, potassium permanganate, a reducing agent, and polyethylene glycol, and stirred at 25-50℃ for 1-5 hours to obtain layered manganese dioxide-coated gold nanoparticles. This invention, by introducing methoxy polyethylene glycol carboxyl groups, prepares a catalyst with unique layered manganese dioxide-coated gold nanoparticles for the photocatalytic conversion of 5-hydroxymethylfurfural.