56 results about "Propagule" patented technology

The invention discloses a leymus chinensis accelerant and a method for repairing grassland by using the leymus chinensis accelerant, and belongs to the technical field of plant accelerant preparation. The method for repairing the grassland through the leymus chinensis accelerant comprises the following steps that P1, the underground part of leymus chinensis is cut up, and a pretreated leymus chinensis bud propagule is obtained; p2, spraying a leymus chinensis accelerant to the pretreated leymus chinensis bud propagules to obtain bud propagule segments; and P3, sowing the propagule section into the natural grassland by using a sowing machine, and repairing the grassland. By spraying the leymus chinensis accelerant, the vigor of leymus chinensis bud propagules can be effectively improved, the growth of leymus chinensis is promoted, and the biomass is increased, so that the restoration of grassland is realized.

Transgenic plants with blue flower color, or their inbred or outbred progeny, or their propagules, partial plant bodies, tissues or cells, are provided. A buckwheat-derived C-glucosyltransferase (CGT) gene or its homolog is transferred into a host plant to cause delphinidin-type anthocyanins and flavone mono-C-glycosides to be copresent in the plant cells.

Disclosed herein are pesticidal compositions comprising effective amounts of Chlorantraniliprole or Cyantraniliprole and Spinetoram, their applications, and a plant propagule comprising such pesticidal compositions.

The invention discloses a tissue culture rapid propagation method for Xanthoceras sorbifolia, which relates to the technical field of biology. The technical key points are: traditional Xanthoceras sorbifolia breeding methods are inefficient, have insufficient seed sources, are difficult to survive after transplantation, and are difficult to preserve excellent traits of the mother plant. By using stem segments and tender stems as explants, the proliferation and rooting of Xanthoceras sorbifolia explants are induced in a short time, the induction rate of callus tissue is improved, browning is reduced, and a solid foundation is laid for the subsequent construction of a rapid propagation system for Xanthoceras sorbifolia.

Provided is a plant treatment method capable of further promoting plant growth using a compound such as ergothioneine. This plant treatment method comprises a step for treating a plant propagule with a compound represented by formula (I) or a tautomer thereof, or an active component that is an agriculturally acceptable salt thereof. [chemical formula 1] (In formula (I), R1 and R2 each independently represent a hydrogen atom or a C1-C4 alkyl group, and R3, R4, and R5 each independently represent a C1-C4 alkyl group.)

The invention discloses an underground propagule composite excitant for ecological restoration and a preparation method thereof, and relates to the technical field of excitant preparation. The fertilizer is prepared from the following raw materials in parts by weight: 0.2 to 0.3 part of gibberellin, 0.2 to 0.5 part of 6-benayl aminopurine, 0.2 to 0.3 part of indolebutyric acid, 0.5 to 0.7 part of sodium nitroprusside, 0.4 to 0.5 part of hydrogen peroxide, 1 to 2 parts of jasmonic acid sustained-release microspheres, 5 to 7 parts of compound amino acid, 1 to 3 parts of compound microbial agent sustained-release microspheres, 2 to 3 parts of seaweed extract, 10 to 13 parts of humic acid, 2 to 3 parts of monopotassium phosphate, 60 to 74 parts of ethanol and 1000 parts of water. The underground propagule composite excitant provided by the invention is beneficial to promoting activation and germination of underground propagules, enhancing plant growth and stress resistance, improving soil micro-ecological functions, forming stable diversity communities and facilitating ecological restoration.

An apparatus for forming a rope matrix having seaweed propagules embedded therein is provided and may include an intertwining assembly configured to intertwine at least three ropes at a braiding point to form a rope matrix. Further, the apparatus may include a conveying device configured to introduce seaweed propagules adjacent to the braiding point and to enable embedding of the seaweed propagules within the rope matrix while the rope matrix is being formed. Further, the apparatus may comprises an offtake assembly configured to move the rope matrix, with the seaweed propagules embedded therein, out of the apparatus.

The invention discloses an application of walnut YX4 as a receptor material in agrobacterium tumefaciens-mediated genetic transformation. The invention relates to the field of genetic transformation, and particularly discloses a method for carrying out genetic transformation by taking Juglans regia YX4 CGMCC (China General Microbiological Culture Collection Center) No.46084 as an acceptor material. According to the invention, genotype walnuts with high somatic embryo induction rate, strong regeneration capacity and high genetic transformation efficiency are screened as genetic transformation receptors, and an efficient genetic transformation system and method are optimized and established. By adopting the transformation method disclosed by the invention, the transformation efficiency (P = 0.008) of agrobacterium infection is remarkably improved. The screened excellent receptor material of the YX-4 genotype is named as YX4, and is preserved in the China General Microbiological Culture Collection Center (CGMCC). The screening of excellent receptor materials and the optimization of a genetic transformation system have important practical significance for establishing a walnut rapid propagation system, and the commercialization process of walnut breeding is promoted.

The invention belongs to the technical field of detection devices, and discloses a detection device and a rapid detection method for enteromorpha microscopic propagules. According to the detection device, a connector and a sample feeding pipe and a connecting pipe at the bottom and the top of a filter are limited through a first protection assembly, a second protection assembly and a limiting assembly respectively, and meanwhile, protection blocks are used for protecting the outer sides of the first protection assembly, the second protection assembly and the filter, so that the filter is prevented from being easily loosened due to vibration during operation of the filter; the rapid detection method comprises the following steps: 1, filtering a collected water sample through a filter membrane in a filter instrument, and extracting high-purity DNA (deoxyribonucleic acid); 2, cloning and purifying the enteromorpha specific gene, determining the DNA concentration of the target gene, and establishing a standard curve; and 3, detecting the copy number of the enteromorpha microcosmic propagule in the water body through qPCR, and calculating the cell abundance according to the copy number of the target gene in a single cell to realize rapid identification and quantitative detection.

The invention discloses a method for constructing a genetic transformation system of cymbidium kanran, which belongs to the technical field of plant genetic engineering, and comprises the following steps of: carrying out monoclonal propagation on rhizomes after germination of cymbidium kanran seeds to obtain vegetative propagules with stable characters, taking the vegetative propagules as transformation receptors, taking GFP (Green Fluorescent Protein) as marker genes, and constructing the genetic transformation system of cymbidium kanran. The method comprises the following steps: adding agrobacterium EHA105 into a YEB liquid culture medium containing 50 mg / L of kanamycin and 25 mg / L of rifampicin as a dip dyeing solution; placing the injured transformation receptor in dip dyeing for transformation, and then carrying out regeneration culture. The invention relates to a method for carrying out genetic transformation on cymbidium kanran by utilizing the innovative receptor and the screening culture medium, and the method can realize efficient and stable genetic transformation without genotype dependence and can solve the problem that the genetic transformation of cymbidium kanran varieties is difficult to carry out. According to the invention, an efficient genetic transformation and regeneration system of cymbidium kanran is established for the first time, and a foundation is laid for verification of related functional genes of cymbidium kanran and further development of biological research of cymbidium transgenic breeding and the like.

The present invention discloses a method for ecological restoration of a sphagnum swamp wetland, comprising the following steps: collecting sphagnum swamp wetland plants and peat soil in the habitat to be restored, moving them to a greenhouse for cultivation, and increasing the number of sphagnum swamp wetland plants; removing invasive plant plants and propagules; cutting off the root zone of the invasive plant and the soil above and below it to construct a restoration groove; keeping the restoration groove in a flooded state to be restored; laying a matrix layer on the restoration groove, and covering the matrix layer with the sphagnum swamp wetland cultivated by the present invention. The present invention rapidly increases the number of sphagnum swamp wetland ecological restoration plants by artificially cultivating the sphagnum swamp growing in the restoration site. In addition, the present invention selects the sphagnum swamp wetland plants and peat soil to be restored, thereby avoiding the ecological differences between the restored and transplanted sphagnum swamp wetland and the sphagnum swamp wetland, and the sphagnum swamp wetland plants used for ecological restoration can adapt to the ecological environment of the restoration site more quickly, with a higher survival rate and a good restoration effect.

The embodiment of the invention relates to a method for restoring a river network type water system ecosystem, which comprises the following steps of: introducing aquatic plant varieties for improving a water environment into nodes and areas which have great influence on water quality in a river network type water system to be restored, so as to form a water quality control type plant framework which stably grows and propagates within 1-3 months; sediment is collected in the river network type water system to be repaired, and species with potential germination ability and density of the species in the propagule bank of the water system are evaluated; supplementing a propagule library: supplementing species lacking propagule resources in the river network type water system to be repaired according to an evaluation result; in the repaired water area with the aquatic plant community growing stably and the water body penetration depth ratio stably kept to be larger than or equal to 0.5, associated plants beneficial to water quality improvement and aquatic plant community stability continue to be introduced. The ecological system has the advantages of being low in construction cost, high in anti-interference performance and excellent in self-maintaining capacity.

The present application belongs to the technical field of plant morphology, and particularly relates to a seed plant propagule three-dimensional shape measurement method based on a video stereomicroscope, comprising: collecting seed plant propagules at the mature stage and air-drying to a constant weight; classifying the propagules according to the seed, fruit shape and surface accessory structure, and determining the measurement criteria of the shape of different categories of propagules; scanning the three-dimensional structure of each category of propagule by using a video stereomicroscope, and accurately measuring the shape parameters length, width and thickness of the propagule; normalizing each category of propagule, calculating the shape index of the propagule, and representing the closeness to a spherical shape. The method quantifies the complex three-dimensional shape characteristics of the seed plant propagule into a comprehensive shape index, realizes accurate quantitative measurement and digitization of the morphological characteristics of the propagule, solves the pain point of determining the shape of the propagule by using a traditional observation tool, and facilitates the comparative identification of the three-dimensional shapes of different categories of seed plant propagules.

The invention belongs to the technical field of microbiological detection and soil biology, and particularly relates to a method for screening arbuscular mycorrhizal fungi in soil, which comprises the following steps: preparing a soil propagule suspension, realizing single individual separation and physical isolation of propagules by using a microecological niche array, and screening the arbuscular mycorrhizal fungi in the soil. The strain is co-cultured with a sterile host root system under a specific abiotic stress condition, and the stress-tolerant functional strain is directly obtained through microscopic monitoring, positive symbiont confirmation and molecular identification. According to the technical scheme, competitive bias voltage can be avoided, the slave mycorrhizal fungi purified strains with low phosphorus, drought or saline-alkali tolerance are efficiently screened out, and screening objectivity and functional targeting are remarkably improved.

This disclosure provides recombinant DNA constructs and transgenic plants having enhanced traits such as increased yield, increased nitrogen use efficiency and enhanced drought tolerance; propagules, progeny and field crops of such transgenic plants; and methods of making and using such transgenic plants. This disclosure also provides methods of producing seed from such transgenic plants, growing such seed and selecting progeny plants with enhanced traits. Also disclosed are transgenic plants with altered phenotypes which are useful for screening and selecting transgenic events for the desired enhanced trait.

The invention relates to a method for rapidly carrying out BSA (Bovine Serum Albumin) gene localization by utilizing a space mutation mutant. The method comprises the following steps: firstly, carrying propagules such as seeds through an aerospace craft, and constructing a broad-spectrum mutant library by utilizing space environment mutagenesis; returning to the ground, culturing and screening M1-generation mutants with target excellent characters, and hybridizing / backcrossing with a wild type to construct a character segregation population; by adopting a group separation analysis (BSA) strategy, selecting phenotype polar differentiation individuals, and respectively mixing the phenotype polar differentiation individuals to form a high-value pool and a low-value pool; and finally, carrying out whole genome high-throughput sequencing on the double pools and the parents, rapidly locking a genome region linked with a target character by combining bioinformatics analysis such as SNP-index and the like, and verifying candidate genes through gene annotation, molecular marking and gene editing. According to the invention, the broad spectrum of space mutagenesis and the high efficiency of the BSA-seq technology are integrated, the period from mutation creation to gene identification is remarkably shortened, and an innovative technical scheme is provided for crop genetic improvement and functional genomics research.

The application provides a compound preparation suitable for organic planting of tomatoes, a preparation method and application, and belongs to the technical field of organic tomato planting, wherein the active ingredient is composed of freshwater unicellular algae and a compound bacterial liquid; the compound bacterial liquid is composed of bacillus subtilis GLB191 and bacillus pumilus GLB197 or is composed of iranian gloeocystidiaceae; the effective viable bacterial number of the bacillus subtilis GLB191 and the bacillus pumilus GLB197 is greater than or equal to 13.5 billion cfu / g ‑1 ; and the propagule number of the iranian gloeocystidiaceae is greater than or equal to 70 pieces / ml ‑1 ; the application can improve the survival rate of tomato seedlings, plays a role in promoting growth, improves the quality of fruits, and has an effective prevention and treatment effect on tomato leaf mold disease.

The invention relates to a degradable three-dimensional habitat protection body and a mangrove propagule planting method based on the three-dimensional habitat protection body, the three-dimensional habitat protection body comprises a plurality of three-dimensional structure units, each three-dimensional structure unit comprises a top wall and a bottom wall, the whole top wall is of a hollow structure arched towards the direction away from the bottom wall, and the bottom wall is of a hollow structure arched towards the direction away from the bottom wall. The whole bottom wall is of a hollow structure which is arched in the direction away from the top wall, and the bottom wall and the top wall jointly define a hollow cavity. In the tide scouring and wave impact process, the protection body can weaken the instantaneous dynamic pressure peak value perpendicular to the hollow structure, and the pull-out force and shear stress acting on seedlings or attached organisms in the cavity are reduced; meanwhile, the flow velocity and internal shear force in the cavity can be reduced, local scouring and sediment erosion are effectively inhibited, a relatively stable matrix environment is formed, and continuous support is provided for propagule germination and root growth.

The invention discloses a plant underground propagule excitant, and belongs to the technical field of excitant preparation. The plant underground propagule excitant is composed of 169.91-196.13 parts of a mixture A and 2-4 parts of an immobilized microbial agent, and the mixture A is composed of the following components in parts by weight: 0.1-0.2 part of brassinolide, 1-2 parts of sodium nitroprusside, 30-40 parts of cane sugar, 70-80 parts of urea, 68-73 parts of disodium hydrogen phosphate and 0.81-0.93 part of a composite bioactive matter. According to the plant underground propagule excitant prepared by the method, the grassland improvement effect exceeds the expectation, the aboveground biomass is increased by 1-2.5 times, the root biomass is increased by 2-3 times, and the density of vegetation plants (clusters) is increased by 2-3 times.

The invention relates to a method for isolating environmental strains of arbuscular mycorrhizal fungi, comprising the following steps: an isolation phase comprising i. the treatment of a soil sample of interest by successive sieving of decreasing size in order to concentrate propagules of arbuscular mycorrhizal fungi present in the soil sample, ii. the trapping of a propagule obtained at the end of the treatment step, in an alginate bead, iii. the culturing of the propagule trapped in an alginate bead, in a solid substrate in contact with a pregerminated plantlet intended to undergo mycorrhizal colonization brought about by said propagule. It is of use in the agronomic field for the purpose of improving the growth and resistance to stresses (biotic and abiotic stresses) of plant species. This invention integrates into an overall context of agricultural transition involving a change in agricultural practices to tend towards growing models less dependent on inputs (water, fertilizers, pesticides).

A coral ring mount device and method for the propagative culture of sessile benthic marine organisms (for example, stony corals) employing either a ring, washer or threaded nut or similar as a propagule mount upon which the coral is set in order to attach, grow and overgrow within a coral nursery system. In the preferred embodiment of the invention, once the coral is grown onto the ring mount and to its requisite size and condition, the ring mount becomes the washer through which passes a screw to secure the ring and coral to the seafloor.

The invention relates to the technical field of strain preservation, and discloses strain preservation equipment with a disinfection function and a disinfection method.The strain preservation equipment comprises a device frame, a primary filtering part is arranged on the device frame, the primary filtering part comprises a primary filtering cover, three sliding rods are connected to the inner wall of the primary filtering cover in a surrounding mode, each sliding rod is of an L-shaped structure, and the primary filtering cover is provided with a secondary filtering part; a tray is connected to the inner wall of a cover opening in the bottom end of the primary filter cover, the top edge of the inner wall of the tray is of an inclined plane structure, caulking grooves corresponding to the three sliding rods are formed in the top of the outer wall of the tray in a surrounding mode, the bent portions of the three sliding rods are inserted into the inner walls of the three caulking grooves respectively, and a pressing disc is arranged in the primary filter cover; the primary filtering part is adopted to filter and intercept impurities and propagules to reduce the sterilization load, the first sterilization part destroys spore structures and preliminarily kills spores by using high-concentration hydrogen peroxide, and the second sterilization part thoroughly inactivates residual spores through high-temperature and high-pressure steam, and accelerates decomposition of hydrogen peroxide at high temperature to avoid chemical residues.

The present application relates to a kind of lake natural ecological restoration method, historical ecological data analysis and ecological system status survey are carried out to the lake to be repaired;Pioneer plant buffer zone is formed by planting floating leaf plants in the center area of the lake to be repaired and / or wind and wave center area;At least one sub-recovery area is set in the lake to be repaired, and all sub-recovery areas are carried out ecological restoration according to preset order, and the measures of ecological restoration include constructing stable primary submersed plant community in each sub-recovery area;Wherein, the primary submersed plant community can stably produce propagule;The propagule produced by each sub-recovery area can be naturally dispersed and planted and spread to other locations outside the sub-recovery area in the lake to be repaired by natural force, to form stable secondary submersed plant community, to expand the repaired area that has been completed;Propagule further disperses, plants and spreads.The outstanding advantages are less investment, fast repair, and suitable for the repair of medium and large lakes.

The invention discloses a salt marsh ecological restoration system based on historical propagule library in-situ activation, and relates to the technical field of ecological restoration, the system comprises a historical habitat identification and calibration module used for identifying and calibrating the historical distribution range of target restoration vegetation in a target area; the deep propagule library verification module is used for carrying out sampling and activity verification on propagules in a sediment deep area and confirming existence of a propagule library capable of being used for in-situ activation; the ecological opportunity decision-making module is used for determining a suitable time window for implementing in-situ activation operation; and the targeted physical disturbance activation module is used for applying non-invasive physical disturbance to the deep area of the sediment, so that the living propagule buried in the deep area floats upwards to the surface layer of the sediment. According to the scheme, normal form transformation from dependence on external transplantation to activation of internal potential is achieved, the repair process is simplified into targeted field operation, and the comprehensive cost and resource consumption are remarkably reduced.

The invention relates to the technical field of biology, and provides a method for rapidly propagating saxifraga ovatus. By collecting leaves for asexual propagation and controlling physical conditions to induce the generation of new plants, the propagation problem of the saussurea ovata is effectively solved, and an effective technical support is provided for introduction, conservation, development and utilization of wild plant resources. The saxifraga ovatus leaves are clustered and compact, the ornamental value is high, the saxifraga ovatus can be used as a shade-tolerant potted plant and can also be applied to an under-forest shady ground cover plant, but due to the fact that the seed collection window period of the saxifraga ovatus is narrow and germination is poor, a new insight and thought are provided for protection of wild plant resources by inducing the leaves to generate asexual propagules and constructing seedlings.

The present invention discloses a rapid propagation method for tissue regeneration and culture of scutellaria baicalensis, belonging to the field of agricultural biotechnology. This method uses scutellaria baicalensis leaves as explants and screens and optimizes the types and ratios of hormones involved in callus induction, callus differentiation, and rooting induction. A rapid propagation system for tissue regeneration and culture of scutellaria baicalensis is successfully constructed, achieving asexual reproduction and obtaining a large number of scutellaria baicalensis regenerated seedlings in a short period of time. This method is simple and easy to operate, has a short planting cycle, and a high survival rate of regenerated plants, facilitating promotion and large-scale planting. This method changes the current situation of scutellaria baicalensis relying on wild resources and provides a theoretical basis and a new method for rapidly obtaining a large number of scutellaria baicalensis plants.

Reproduction 1.1 is a top, front perspective view of a first embodiment of a trap and article for destruction of pest according to the new design; reproduction 1.2 is a back elevation view of the embodiment of reproduction 1.1; reproduction 1.3 is a front elevation view of the embodiment of reproduction 1.1; reproduction 1.4 is a left side elevation view of the embodiment of reproduction 1.1; reproduction 1.5 is a right side elevation view of the embodiment of reproduction 1.1; reproduction 1.6 is a top plan view of the embodiment of reproduction 1.1; reproduction 1.7 is a bottom plan view of the embodiment of reproduction 1.1; reproduction 1.8 is a back, bottom perspective view of the embodiment of reproduction 1.1; reproduction 2.1 is a top, front perspective view of a second embodiment of an insect trap according to the new design; reproduction 2.2 is a back elevation view of the embodiment of reproduction 2.1; reproduction 2.3 is a front elevation view of the embodiment of reproduction 2.1; reproduction 2.4 is a left side elevation view of the embodiment of reproduction 2.1; reproduction 2.5 is a right side elevation view of the embodiment of reproduction 2.1; reproduction 2.6 is a top plan view of the embodiment of reproduction 2.1; reproduction 2.7 is a bottom plan view of the embodiment of reproduction 2.1; reproduction 2.8 is a back, bottom view of the embodiment of reproduction 2.1; reproduction 3.1 is a top, front perspective view of a third embodiment of a trap and article for destruction of pest according to the new design; reproduction 3.2 is a back elevation view of the embodiment of reproduction 3.1; reproduction 3.3 is a front elevation view of the embodiment of reproduction 3.1; reproduction 3.4 is a left side elevation view of the embodiment of reproduction 3.1; reproduction 3.5 is a right side elevation view of the embodiment of reproduction 3.1; reproduction 3.6 is a top plan view of the embodiment of reproduction 3.1; reproduction 3.7 is a bottom plan view of the embodiment of reproduction 3.1; reproduction 3.8 is a back, bottom view of the embodiment of reproduction 3.1; reproduction 4.1 is a top, front perspective view of a fourth embodiment of trap and article for destruction of pest according to the new design; reproduction 4.2 is a back elevation view of the embodiment of reproduction 4.1; reproduction 4.3 is a front elevation view of the embodiment of reproduction 4.1; reproduction 4.4 is a left side elevation view of the embodiment of reproduction 4.1; reproduction 4.5 is a right side elevation view of the embodiment of reproduction 4.1; reproduction 4.6 is a top plan view of the embodiment of reproduction 4.1; reproduction 4.7 a bottom plan view of the embodiment of reproduction 4.1; reproduction 4.8 is a back, bottom view of the embodiment of reproduction 4.1; reproduction 5.1 is a top, front perspective view of a fifth embodiment of a trap and article for destruction of pest according to the new design; reproduction 5.2 is a back elevation view of the embodiment of reproduction 5.1; reproduction 5.3 is a front elevation view of the embodiment reproduction 5.1; reproduction 5.4 is a left side elevation view of the embodiment of reproduction 5.1; reproduction 5.5 is a right side elevation view of the embodiment of reproduction 5.1; reproduction 5.6 is a top plan view of the embodiment of reproduction 5.1; reproduction 5.7 is a bottom plan view of the embodiment of reproduction 5.1; and reproduction 5.8 is a back, bottom view of the embodiment of reproduction 5.1; the broken lines shown in the drawings illustrate portions of the trap and article for destruction of pest that form no part of the claimed design.

The utility model relates to an auxiliary device for field planting and growth of submerged plants. The auxiliary device comprises a mesh bag, a traction rope and a balancing weight. The mesh bag is provided with an opening and can wrap propagule seedlings of submerged plants. The first end of the traction rope binds and tightens the opening of the mesh bag wrapping the propagule seedlings, the second end of the traction rope is connected with the balancing weight, and the weight of the balancing weight is larger than the overall weight of the propagule seedlings and the mesh bag. The submerged plant field planting growth auxiliary device has the beneficial effects that the stability of wrapped propagule seedlings for resisting low light stress and disturbance of the water body can be improved, the growth of the propagule seedlings is promoted, the purpose of ecological restoration of the water body is achieved, and the submerged plant field planting growth auxiliary device is particularly suitable for ecological restoration of the water body with strong disturbance and poorer illumination conditions. And furthermore, the structure is simple, the cost is relatively low, and the preparation is convenient.

The invention relates to a grafting technology for efficiently cultivating high-quality propagule nursery stocks of Machilus pauhoi. From the middle ten days of December to the middle ten days of January of the next year, selecting a 2-year-old excellent machilus pauhoi seedling which grows robustly and is free of plant diseases and insect pests and has the ground diameter of more than 1.2 cm as a stock, and taking a current-year-old branchlet which grows normally, is free of plant diseases and insect pests, has the diameter of 0.4-0.5 cm and is lignified, sap starts to flow and the phloem is easy to peel off at the middle upper part of other excellent scion-picking seed trees as a scion; cutting the rootstock and cutting the scion, inserting the scion into the rootstock notch, and binding and fixing the rootstock notch and the scion cutting surface; after grafting is carried out for 20-25 days, when axillary buds of the scions grow to 1.8-2.2 cm, binding bands for binding the scions are removed; removing the binding band for binding the rootstock 45 days after grafting; cutting off coppice shoots on the rootstock after new twigs of the scions grow to 20-25 cm. The method has the beneficial effects that the grafting survival rate of the Machilus pauhoi can be improved, the excellent hereditary characteristics of the stock and the fruiting seed tree can be fused by utilizing the grafting technology, and the high-quality propagule nursery stock of the Machilus pauhoi can be efficiently and creatively cultivated.

The invention relates to a passive active oxygen generating device which is characterized by comprising a shell, a substrate and a reaction film which are arranged in sequence. Visible light in the environment enters the reaction film and is absorbed by the reaction film, a large number of vacancies and electrons are generated, and the vacancies and electrons react with water or oxygen molecules in the surrounding environment to generate active oxygen. The active oxygen is a broad-spectrum bactericide, can kill bacterial propagules, bacterial spores, viruses and fungi, also has a good killing effect on protozoa and oocysts thereof, and also can destroy bacterial toxins, hepatitis B surface antigens and the like. The device can be applied to sterilization and disinfection of object surfaces, air and the like, and has the advantages of no need of additional energy sources, high efficiency, lasting performance and the like.