196 results about "Propagule" patented technology

The invention relates to an application method of arbuscular mycorrhizal fungus in large-scale tobacco cultivation. A primary arbuscular mycorrhizal fungi fungicide is inoculated and propagated separately, and propagule spore density of an arbuscular mycorrhiza of each fungi after the propagation is 30 pieces per gram of dry soil. A float breeding method is adopted to conduct arbuscular mycorrhizal fungi fungicide combinedinoculation to tobacco, mycorrhization tobacco seedlings are obtained, and the mycorrhization tobacco seedlings are directly applied to field production. When being transplanted, the mycorrhization tobacco seedlings are conducted with a two-step inoculation, and the mixed fungicide is mixed by propagated acaulospora mellea, glomus mosseae and glomus intraradices at a proportion of 0.5-1: 1:1. A colony formed by the propagation of the arbuscular mycorrhizal fungus is close to a natural state, and fungus cooperate and play a better role of growth promoting and quality improving. According to the application method of the arbuscular mycorrhizal fungus in the large-scale tobacco cultivation, by means of the two times of inoculations of the arbuscular mycorrhizal fungus, the proportion of arbuscular mycorrhizal fungus and the tobacco in a symbiotic system built during a seedling stage of the tobacco is guaranteed, and the beneficial effects of the symbiotic system to the development of the tobacco seedlings are ensured. The application method of the arbuscular mycorrhizal fungus in the large-scale tobacco cultivation is simple and practical in method and environment friendly.

A planting system involving a non-damaging stimulus (3) which is placed in the vicinity of a propagule (2) causes a naturally defensive substance (5) to be produced by the propagule. This substance may exist within an encapsulant (1) for a period of time so that the propagule may have enhanced disease control until it develops sufficiently to fend for itself. In one embodiment a large encapsulant surrounds a potato tuber. This encapsulant may include chitin to cause the release of chitinase through intermediate stimulants such as mRNA and activated carbon to absorb or release particular substances. The encapsulant is bound through the use of pentosan which acts with flaked chitin to achieve an outer casing and further protect the propagule both mechanically and chemically.

A water eco-environment protection method based on water plant harvesting. The invention relates to a water eco-environment protection method, which is characterized in that water plants are cultivated in a water body or naturally recovered water plants are utilized; nitrogen and phosphorous nutrients are absorbed by water plant growth; the water plants are harvested in growth period by manual work or waterweed harvesting equipment so as to promote the regrowth of the water plants; harvesting is carried out for multiple times, ends in the growth period and is carried out for the last time after the water plants grow seeds or vegetative propagule so as to extract the nitrogen and phosphorus nutrients from water in the form of water plant residues. The invention aims to maximize the accumulated biomass of the water plants during the whole growth period or optimize the water quality purifying effect and simultaneously remove nutrients in the water body and deposits, alleviate the eutrophication process of the water body and prevent the water plants from decaying and polluting the water body once again. The method provided by the invention is suitable for daily ecological maintenance of shallow lakes, urban landscape water, slow flow rivers, small reservoirs, wetlands and ponds.

The invention provides an ecological reconstruction method for the plant diversity after returning of an abandoned farmland to a marsh wetland, and relates to a method for reconstructing the plant diversity of the marsh wetland.The method is used for solving the problem that after the process of returning the abandoned farmland to the marsh wetland, the plant diversity of the abandoned farmland marsh wetland is difficult to recover.The method comprises the steps of 1 abandoned farmland surface soil removing; 2 natural wetland soil propagule bank collecting; 3 abandoned farmland soil propagule bank introducing; 4 hydrological controlling of the abandoned recovery farmland; 5 non-target species removing in the initial stage of reconstruction.The method is simple, convenient and high in reconstruction speed, can be widely applied to ecological reconstruction of the abandoned farmland marsh wetland in the north and is suitable for reconstructing the plant diversity of the marsh wetland.

Disclosed is an insecticidal composition comprising by weight based on the total weight of the composition:

• • (a) from about 9 to about 91% of one or more anthranilic diamide insecticides; and
  • (b) from about 9 to about 91% of a nonionic ethylene oxide-propylene oxide block copolymer component having a water solubility of at least about 5% by weight at 20° C., a hydrophilic-lipophilic balance value of at least about 5 and an average molecular weight ranging from about 1500 to about 20000 daltons;
  • wherein the ratio of component (b) to component (a) is about 1:10 to about 10:1 by weight.

Also disclosed is a geotropic propagule coated with an insecticidally effective amount of the aforedescribed composition. Further disclosed is a liquid composition consisting of about 5 to 80 weight % of the aforedescribed composition and about 20 to 95 weight % of a volatile aqueous liquid carrier, and a method for protecting a geotropic propagule and plant derived therefrom from a phytophagous insect pest, the method comprising coating the propagule with an insecticidally effective amount of the aforedescribed liquid composition and then evaporating the volatile aqueous liquid carrier.

The invention discloses wettable powder of a streptomyces roseoflavus biological bactericide, which adopts diatomite as a matrix and comprises the following components: not less than 108 propagules of streptomyces roseoflavus strain 0116, and 4% of a wetting dispersant. It is determined that diatomite is used as a carrier and sodium lignosulphonate is used as the wetting dispersant. In addition, the strain wettable powder storage stability is determined by regular detection of the viable bacterial count, and results show that after strain wettable powder storage for 24 months, the viable bacterial count is more than 80%. The invention has the characteristics of short development period, low cost, simple process, and capability of being developed into various dosage forms, and is suitable for large-scale production. Since propagules of streptomyces roseoflavus strain 0116 is used as the main component of the wettable powder, the invention is suitable for large-scale production, and when compared with strain fermentation liquor, the invention greatly shortens the field using amount, and reduces packaging and transport cost.

The invention discloses a grifola high-yield cultivation technology, and belongs to the technical field of cultivation. A method comprises the following steps: selecting and judging strains, selecting grifola wild funguses or propagules within two generations, evaluating and cultivating associated funguses, selecting robust and vigorous associated fungus strains to be cultivated, separating and purifying honey fungus strains from sclerotia, then conducting cultivation, and cultivating associated fungus sticks. According to the cultivation method, the scientific mode that the honey fungus strains and grifola 3+2 are arranged in an inclined mode is adopted, namely, the configuration structure that the three layers of honey fungus sticks are added on the two layers of the grifola strains is adopted, and the fungus stick plane inclined bodies are configurated in a staggered mode between layers. Cultivation ponding can shorten the grifola cultivation period by more than 2 years, the three-year period from planting to harvesting is reached, the high-yield effect that the output ratio is improved to be 1:10-15 is achieved, the production technology is stable, the grifola quality is high and safe, and the technical problems that the grifola artificial cultivation period is long, the production is unstable and the high-yield production can not be achieved for a long time are solved.

A transplanting system including both methods and specific types of transplant apparatus such as propagule transplant trays(3, 4, 5, 6) addresses the need for the use of a propagule punch machine (8) on a variety of adolescent tray configurations (9, 11, 12, e.g.) and the increased operational efficiency that such enhanced operational capability affords. The system may involve at least one areally continuous propagule tray (3, 4, 5, 6) that is operationally compatible with a numerically significant number of adolescent plant trays. Other embodiments of the invention disclose punch pins (59) for use in a plant punch machine transplanter that are user-detachable and differentially designed so that plant punch elements of a certain design may be installed as part of the machine to customize the transplant process for a given species and transplant modality. The invention also provides for a consultable collection (such as printed documents or an electronic database, e.g.) of plant transplant process information usable to increase transplant process efficiency.

Aiming at the defects of conidiospores as propagula of an insecticide of insect pathogenic fungi, the invention provides a technology of liquid culture of entomogenous fungus sclerotia, and the entomogenous fungus can be beauveria bassiana. The liquid culture method of entomogenous fungus sclerotia provided by the invention is required to control the liquid culture components and the culture conditions within a certain range. The cultured sclerotia can be stored for a long time after vacuum drying, and can still germinate mycelia and pathogenic conidiospores under suitable conditions. The micro-sclerotia prepared by drying have maintained activity, can germinate mycelia and spores after rehydration, and increase the potential of beauveria bassiana as a biocontrol factor for western flowerthrips in a soil-dwelling phase. The technology has low cost, long storage period of sclerotia, and is quite suitable for popularization.

Previous research demonstrated that aflatoxin contamination in corn is reduced by field application of wheat grains pre-inoculated with the non-aflatoxigenic Aspergillus flavus strain NRRL 30797. To facilitate field applications of the biocontrol isolate, a series of laboratory studies were conducted on the reliability and efficiency of replacing wheat grains with the novel bioplastic formulation Mater-Bi® to serve as a carrier matrix to formulate this fungus. Mater-Bi® granules were inoculated with a conidial suspension of NRRL 30797 to achieve a final cell density of ˜log 7 conidia/granule. Incubation of 20-g soil samples receiving a single Mater-Bi® granule for 60-days resulted in log 4.2 to 5.3 propagules of A. flavus/g soil for microbiologically active and sterilized soil, respectively. Increasing the number of granules had no effect on the degree of soil colonization by the biocontrol fungus. In addition to the maintenance of rapid vegetative growth and colonization of soil samples, the bioplastic formulation was highly stable, indicating that Mater-Bi® is a suitable substitute for biocontrol applications of A. flavus NRRL 30797.

The invention discloses a method for creating easily-industrialized breeding Cymbidium sinense resources, belonging to the field of innovation of plant germplasm resources. The method comprises the following steps: selecting hybrid Cymbidium containing a Cymbidium sinense genome as core parents so as to prepare a hybrid combination, carrying out single-seed in-vitro culturing and evaluating the type and the tissue culturing and rapid propagation abilities of an intermediate propagule of hybrid generations, producing test-tube seedlings, identifying produced test-tube seedlings, and carrying out transplanting so as to obtain the easily-industrialized breeding Cymbidium sinense resources. Compared with a conventional breeding method which selects Cymbidium sinense resources after transplanting of grown-up seedlings, the method provided by the invention completes selection of the easily-industrialized Cymbidium sinense resources in test tubes, so the period of breeding is significantly shortened, and manpower and material resources are saved; meanwhile, the method can really improve the tissue culturing and rapid propagation abilities of traditional Cymbidium sinense, obtains the easily-industrialized breeding Cymbidium sinense resources, realizes industrialized production of Cymbidium sinense seedlings, and has important practical significance on promoting the industrial development of Cymbidium sinense.

A method and apparatus for the regeneration, acclimation and conditioning of in vitro derived plant propagules is presented. The apparatus comprises a regulated chamber, a laminar flow work station coupled to the regulated environment, and a supply compartment. A process control system regulates the chamber to sterile or near sterile conditions. The process control system monitors and modulates the microenvironment, nutritional profile and frequency of fertigation, atmospheric composition, as well as the quality and quantity of irradiance incident on propagules in the sterile chamber. This controlled environ optimizes propagules regeneration, and facilitates the establishment of photoautotrophism and homeostatic capacity of plant tissues. Furthermore, the apparatus allows for the metabolic and morphologic conditioning of plantlets for high intensity indoor cultivation as well as the inoculation of plantlets with beneficial bacteria and fungi which increase metabolic function and provide a protection from colonization of plantlets by pathogenic microflora through spatial occupation.

The invention provides a method for restoring submerged plant communities by utilizing a three-dimensional plant net. The method comprises the following steps: taking plants as raw materials, and weaving the three-dimensional plant net; and culturing submerged plants by utilizing the three-dimensional plant net, forming a submerged plant module, and optimizing the planting composition so as to restore the submerged plant communities in a damaged river-lake ecosystem. A submerged plant seed bank serves as a raw material, the propagule has high propagation efficiency under controllable conditions after the seeds germinate and grow to a certain length, the survival rate is higher than that of transplanting of submerged plants, and the field planting ratio of the plants is higher than that of direct dripping of the propagule. The method provided by the invention has the advantages that the raw materials are selected from aquatic plants, secondary pollution is avoided, the novel utilization pathway of the aquatic plants can be developed, and the condition that nitrogen and phosphorus loads in a water body are increased due to rot of the aquatic plants can be reduced; due to the three-dimensional plant net, the damage on the submerged plants is reduced by virtue of overall transplanting, the survival rate is improved, and planting of stonewort and other submerged plants which are slender and difficult to transplant can be facilitated; and moreover, the plant net has a certain net diameter, the plant root system can be in contact with bottom mud through meshed holes, and field planting of the plants is not influenced.

The invention relates to a method of preparing a bioactive substance with co-cultivation of a plant-based raw material and endophytic bacteria. The method includes the steps of: 1) pretreatment to the plant-based raw material: preparing sterile-cultured propagules, such as living plant cells and tissues, or drying and/or performing pulverization to the plant-based raw material, and performing sterilization pretreatment; 2) activation to the endophytic bacteria: inoculating the endophytic bacteria on a microbial culture medium to perform pre-cultivation for 1-25 days to produce activated endophytic bacteria; 3) co-cultivation of the bio-material and the endophytic bacteria: in a co-cultivation device, inoculating the activated endophytic bacteria in the step 2) and adding a solid or liquid culture medium, which contains the plant-based raw material and is prepared in the step 1), to perform mixed cultivation for 4-90 days to obtain a co-cultivation product; and 4) extraction and preparation of the bioactive substance. Through the co-cultivation of the plant-based raw material and the endophytic bacteria, the bioactive substance is prepared. The method is simple and controllable, achieves stable expression of the product and is short in production period.

The invention relates to a symbiotic germination method of Chinese cymbidium hybrid seeds and bacteria, and the symbiotic germination inoculation is performed under there conditions: (a) after seeds are sowed, bacteria are inoculated immediately, and the mode of immediate inoculation can reduce the germination time of the seeds, thus the seeds can germinate early, the germination rate is high, and sprouts are regular; (b) if the aseptic sowing effect is poor, namely no seeds or few seeds germinate more than one year, the bacteria are inoculated when the sprouts are irregular, the bacteria are inoculated for 4-13 months as long as the seeds does not lose viability, then a large amount of seeds germinate for 4-10 months; and (c) the aseptic germination and the bacterial symbiotic germination are simultaneously adopted in the sowing process, wherein for the hybrid seeds which are easy to germinate, the aseptic intermediate propagule can be obtained simultaneously for aseptic culture conveniently; and for the hybrid seeds which are difficult to germinate or have poor germination effect, the invention can also ensure that the ideal germination effect can be obtained in the shortest time. The intermediate propagule which grows after the symbiotic germination with the bacteria is in a rhizomatic shape, can be cultured with the bacteria, and can be also cultured aseptically after the bacteria are removed.

The invention provides a primer for detecting the soybean phytophthora, and a kit and a method thereof. The primer and the detecting method for detecting the soybean phytophthora have high accuracy, high specificity and high sensitivity, and can detect the various propagules of the soybean phytophthora such as hypha, oospore, zoospore and the like. The whole technique is fast, sensitive and accurate, which has important significance in the prevention of foreign soybean phytophthora, the detection of pathogenic bacteria in domestic affected area, the international trade and the like.

The invention provides a method for improving compacted yellow clay in an open waste dump. The method includes steps: well mixing sandy soil with the yellow clay according to a mass ratio of 1:1-3:1 to obtain a soil matrix; planting local adaptable plants in the soil matrix; performing inoculation of arbuscular mycorrhizal fungi propagules in the soil matrix at the root of each plant; cultivatingthe plants to realize fungi-plant symbiosis. By mixing of the sandy soil with the yellow clay according to a certain ratio, the method has advantages of convenience and quickness in soil acquisition,extensive sources, low cost and suitableness for quick structural improvement of the compacted yellow clay in the local open waste dump are realized; by inoculation of arbuscular mycorrhizal fungi, the vegetation survival rate and biomass can be increased, soil improvement is realized without negative effects, and the like.

The invention relates to a rapid propagation method of cassava virus-free seedlings. The method comprises the following steps: step one, collecting explants and carrying out a detoxification pretreatment; step two, disinfecting the surfaces of explants and taking off the stem tips; step three, inducing the stem tips to obtain seedlings; step four, subjecting the seedlings to subculture multiplication culture; step five, carrying out virus detection through a semi-quantitative RT-PCR method; step six, domesticating and transplanting the virus-free tube seedlings. The provided method can effectively remove various viruses and bacteria taking cassava stems as the nutrient source, and obtains virus-free cassava seedlings. The healthy planting, purification and rejuvenation of cassava seedling can be achieved. The occupied space for propagation is small, the propagation speed is quick, and the survival rate of transplanting is high. The provided method can be applied to safe species exchange among different countries and areas, can provide healthy cassava seedlings, reduces the happening and spreading of diseases, and provides technical guarantees for sustainable development of cassava.

The invention relates to the field of plant cultivation, and specifically discloses a plant conservation device and a use method. The device is formed by a conservation cover, a base basin, a planting basin, and a water-absorbing material. The device is characterized in that the a cultured plant or a propagule of a plant are planted in the planting basin, and then the planting basin is put on a supporter which is raised in the base basin, and the base basin is held with a culture solution, a transparent conservation cover is covered, so the cultured plant is in a relative closed and moist conservation space, leaves are kept lively and can normally perform photosynthesis to synthetize organic nutrition for plants to grow or for propagule to take roots and sprout and grow into plants while moisture supply is satisfied. Compared with the prior art, the device is advantaged by simple structure, convenient use operation, not high seedling raising technology, few and even no daily nurse, labor saving, water saving, and energy conversation, high seedling survival rate, and low seedling growing cost, and the plants do not easily interfered by external environment, and do not easily be damaged by diseases and pests. The device and the method can be widely applied in fields of plant cutting seedling growing, grafting seedling growing, seedling transplanting in-cup maintenance, and plant off-ground health preserving and fresh keeping.