27 results about "Propagule" patented technology

The invention discloses an underground propagule composite excitant for ecological restoration and a preparation method thereof, and relates to the technical field of excitant preparation. The fertilizer is prepared from the following raw materials in parts by weight: 0.2 to 0.3 part of gibberellin, 0.2 to 0.5 part of 6-benayl aminopurine, 0.2 to 0.3 part of indolebutyric acid, 0.5 to 0.7 part of sodium nitroprusside, 0.4 to 0.5 part of hydrogen peroxide, 1 to 2 parts of jasmonic acid sustained-release microspheres, 5 to 7 parts of compound amino acid, 1 to 3 parts of compound microbial agent sustained-release microspheres, 2 to 3 parts of seaweed extract, 10 to 13 parts of humic acid, 2 to 3 parts of monopotassium phosphate, 60 to 74 parts of ethanol and 1000 parts of water. The underground propagule composite excitant provided by the invention is beneficial to promoting activation and germination of underground propagules, enhancing plant growth and stress resistance, improving soil micro-ecological functions, forming stable diversity communities and facilitating ecological restoration.

The invention belongs to the technical field of detection devices, and discloses a detection device and a rapid detection method for enteromorpha microscopic propagules. According to the detection device, a connector and a sample feeding pipe and a connecting pipe at the bottom and the top of a filter are limited through a first protection assembly, a second protection assembly and a limiting assembly respectively, and meanwhile, protection blocks are used for protecting the outer sides of the first protection assembly, the second protection assembly and the filter, so that the filter is prevented from being easily loosened due to vibration during operation of the filter; the rapid detection method comprises the following steps: 1, filtering a collected water sample through a filter membrane in a filter instrument, and extracting high-purity DNA (deoxyribonucleic acid); 2, cloning and purifying the enteromorpha specific gene, determining the DNA concentration of the target gene, and establishing a standard curve; and 3, detecting the copy number of the enteromorpha microcosmic propagule in the water body through qPCR, and calculating the cell abundance according to the copy number of the target gene in a single cell to realize rapid identification and quantitative detection.

The invention discloses a method for constructing a genetic transformation system of cymbidium kanran, which belongs to the technical field of plant genetic engineering, and comprises the following steps of: carrying out monoclonal propagation on rhizomes after germination of cymbidium kanran seeds to obtain vegetative propagules with stable characters, taking the vegetative propagules as transformation receptors, taking GFP (Green Fluorescent Protein) as marker genes, and constructing the genetic transformation system of cymbidium kanran. The method comprises the following steps: adding agrobacterium EHA105 into a YEB liquid culture medium containing 50 mg / L of kanamycin and 25 mg / L of rifampicin as a dip dyeing solution; placing the injured transformation receptor in dip dyeing for transformation, and then carrying out regeneration culture. The invention relates to a method for carrying out genetic transformation on cymbidium kanran by utilizing the innovative receptor and the screening culture medium, and the method can realize efficient and stable genetic transformation without genotype dependence and can solve the problem that the genetic transformation of cymbidium kanran varieties is difficult to carry out. According to the invention, an efficient genetic transformation and regeneration system of cymbidium kanran is established for the first time, and a foundation is laid for verification of related functional genes of cymbidium kanran and further development of biological research of cymbidium transgenic breeding and the like.

The present application belongs to the technical field of plant morphology, and particularly relates to a seed plant propagule three-dimensional shape measurement method based on a video stereomicroscope, comprising: collecting seed plant propagules at the mature stage and air-drying to a constant weight; classifying the propagules according to the seed, fruit shape and surface accessory structure, and determining the measurement criteria of the shape of different categories of propagules; scanning the three-dimensional structure of each category of propagule by using a video stereomicroscope, and accurately measuring the shape parameters length, width and thickness of the propagule; normalizing each category of propagule, calculating the shape index of the propagule, and representing the closeness to a spherical shape. The method quantifies the complex three-dimensional shape characteristics of the seed plant propagule into a comprehensive shape index, realizes accurate quantitative measurement and digitization of the morphological characteristics of the propagule, solves the pain point of determining the shape of the propagule by using a traditional observation tool, and facilitates the comparative identification of the three-dimensional shapes of different categories of seed plant propagules.

The invention belongs to the technical field of microbiological detection and soil biology, and particularly relates to a method for screening arbuscular mycorrhizal fungi in soil, which comprises the following steps: preparing a soil propagule suspension, realizing single individual separation and physical isolation of propagules by using a microecological niche array, and screening the arbuscular mycorrhizal fungi in the soil. The strain is co-cultured with a sterile host root system under a specific abiotic stress condition, and the stress-tolerant functional strain is directly obtained through microscopic monitoring, positive symbiont confirmation and molecular identification. According to the technical scheme, competitive bias voltage can be avoided, the slave mycorrhizal fungi purified strains with low phosphorus, drought or saline-alkali tolerance are efficiently screened out, and screening objectivity and functional targeting are remarkably improved.

The invention relates to a method for rapidly carrying out BSA (Bovine Serum Albumin) gene localization by utilizing a space mutation mutant. The method comprises the following steps: firstly, carrying propagules such as seeds through an aerospace craft, and constructing a broad-spectrum mutant library by utilizing space environment mutagenesis; returning to the ground, culturing and screening M1-generation mutants with target excellent characters, and hybridizing / backcrossing with a wild type to construct a character segregation population; by adopting a group separation analysis (BSA) strategy, selecting phenotype polar differentiation individuals, and respectively mixing the phenotype polar differentiation individuals to form a high-value pool and a low-value pool; and finally, carrying out whole genome high-throughput sequencing on the double pools and the parents, rapidly locking a genome region linked with a target character by combining bioinformatics analysis such as SNP-index and the like, and verifying candidate genes through gene annotation, molecular marking and gene editing. According to the invention, the broad spectrum of space mutagenesis and the high efficiency of the BSA-seq technology are integrated, the period from mutation creation to gene identification is remarkably shortened, and an innovative technical scheme is provided for crop genetic improvement and functional genomics research.

The application provides a compound preparation suitable for organic planting of tomatoes, a preparation method and application, and belongs to the technical field of organic tomato planting, wherein the active ingredient is composed of freshwater unicellular algae and a compound bacterial liquid; the compound bacterial liquid is composed of bacillus subtilis GLB191 and bacillus pumilus GLB197 or is composed of iranian gloeocystidiaceae; the effective viable bacterial number of the bacillus subtilis GLB191 and the bacillus pumilus GLB197 is greater than or equal to 13.5 billion cfu / g ‑1 ; and the propagule number of the iranian gloeocystidiaceae is greater than or equal to 70 pieces / ml ‑1 ; the application can improve the survival rate of tomato seedlings, plays a role in promoting growth, improves the quality of fruits, and has an effective prevention and treatment effect on tomato leaf mold disease.

The invention relates to a degradable three-dimensional habitat protection body and a mangrove propagule planting method based on the three-dimensional habitat protection body, the three-dimensional habitat protection body comprises a plurality of three-dimensional structure units, each three-dimensional structure unit comprises a top wall and a bottom wall, the whole top wall is of a hollow structure arched towards the direction away from the bottom wall, and the bottom wall is of a hollow structure arched towards the direction away from the bottom wall. The whole bottom wall is of a hollow structure which is arched in the direction away from the top wall, and the bottom wall and the top wall jointly define a hollow cavity. In the tide scouring and wave impact process, the protection body can weaken the instantaneous dynamic pressure peak value perpendicular to the hollow structure, and the pull-out force and shear stress acting on seedlings or attached organisms in the cavity are reduced; meanwhile, the flow velocity and internal shear force in the cavity can be reduced, local scouring and sediment erosion are effectively inhibited, a relatively stable matrix environment is formed, and continuous support is provided for propagule germination and root growth.

The invention relates to a method for isolating environmental strains of arbuscular mycorrhizal fungi, comprising the following steps: an isolation phase comprising i. the treatment of a soil sample of interest by successive sieving of decreasing size in order to concentrate propagules of arbuscular mycorrhizal fungi present in the soil sample, ii. the trapping of a propagule obtained at the end of the treatment step, in an alginate bead, iii. the culturing of the propagule trapped in an alginate bead, in a solid substrate in contact with a pregerminated plantlet intended to undergo mycorrhizal colonization brought about by said propagule. It is of use in the agronomic field for the purpose of improving the growth and resistance to stresses (biotic and abiotic stresses) of plant species. This invention integrates into an overall context of agricultural transition involving a change in agricultural practices to tend towards growing models less dependent on inputs (water, fertilizers, pesticides).

The invention relates to the technical field of strain preservation, and discloses strain preservation equipment with a disinfection function and a disinfection method.The strain preservation equipment comprises a device frame, a primary filtering part is arranged on the device frame, the primary filtering part comprises a primary filtering cover, three sliding rods are connected to the inner wall of the primary filtering cover in a surrounding mode, each sliding rod is of an L-shaped structure, and the primary filtering cover is provided with a secondary filtering part; a tray is connected to the inner wall of a cover opening in the bottom end of the primary filter cover, the top edge of the inner wall of the tray is of an inclined plane structure, caulking grooves corresponding to the three sliding rods are formed in the top of the outer wall of the tray in a surrounding mode, the bent portions of the three sliding rods are inserted into the inner walls of the three caulking grooves respectively, and a pressing disc is arranged in the primary filter cover; the primary filtering part is adopted to filter and intercept impurities and propagules to reduce the sterilization load, the first sterilization part destroys spore structures and preliminarily kills spores by using high-concentration hydrogen peroxide, and the second sterilization part thoroughly inactivates residual spores through high-temperature and high-pressure steam, and accelerates decomposition of hydrogen peroxide at high temperature to avoid chemical residues.

The present application relates to a kind of lake natural ecological restoration method, historical ecological data analysis and ecological system status survey are carried out to the lake to be repaired;Pioneer plant buffer zone is formed by planting floating leaf plants in the center area of the lake to be repaired and / or wind and wave center area;At least one sub-recovery area is set in the lake to be repaired, and all sub-recovery areas are carried out ecological restoration according to preset order, and the measures of ecological restoration include constructing stable primary submersed plant community in each sub-recovery area;Wherein, the primary submersed plant community can stably produce propagule;The propagule produced by each sub-recovery area can be naturally dispersed and planted and spread to other locations outside the sub-recovery area in the lake to be repaired by natural force, to form stable secondary submersed plant community, to expand the repaired area that has been completed;Propagule further disperses, plants and spreads.The outstanding advantages are less investment, fast repair, and suitable for the repair of medium and large lakes.

The invention discloses a salt marsh ecological restoration system based on historical propagule library in-situ activation, and relates to the technical field of ecological restoration, the system comprises a historical habitat identification and calibration module used for identifying and calibrating the historical distribution range of target restoration vegetation in a target area; the deep propagule library verification module is used for carrying out sampling and activity verification on propagules in a sediment deep area and confirming existence of a propagule library capable of being used for in-situ activation; the ecological opportunity decision-making module is used for determining a suitable time window for implementing in-situ activation operation; and the targeted physical disturbance activation module is used for applying non-invasive physical disturbance to the deep area of the sediment, so that the living propagule buried in the deep area floats upwards to the surface layer of the sediment. According to the scheme, normal form transformation from dependence on external transplantation to activation of internal potential is achieved, the repair process is simplified into targeted field operation, and the comprehensive cost and resource consumption are remarkably reduced.

The utility model relates to an auxiliary device for field planting and growth of submerged plants. The auxiliary device comprises a mesh bag, a traction rope and a balancing weight. The mesh bag is provided with an opening and can wrap propagule seedlings of submerged plants. The first end of the traction rope binds and tightens the opening of the mesh bag wrapping the propagule seedlings, the second end of the traction rope is connected with the balancing weight, and the weight of the balancing weight is larger than the overall weight of the propagule seedlings and the mesh bag. The submerged plant field planting growth auxiliary device has the beneficial effects that the stability of wrapped propagule seedlings for resisting low light stress and disturbance of the water body can be improved, the growth of the propagule seedlings is promoted, the purpose of ecological restoration of the water body is achieved, and the submerged plant field planting growth auxiliary device is particularly suitable for ecological restoration of the water body with strong disturbance and poorer illumination conditions. And furthermore, the structure is simple, the cost is relatively low, and the preparation is convenient.

The invention provides a non-classical terpenoid cyclase for biosynthesis of trichoderma diterpenoid compounds harzianol I and wickerol A, a coding gene of the non-classical terpenoid cyclase, and a biological function of the non-classical terpenoid cyclase and the harzianol I for regulating and controlling a fungal resistant propagule. Belongs to the technical field of natural product biosynthesis. The non-classical terpenoid cyclase TriDTCs disclosed by the invention is a brand new terpenoid synthase family, and can be used for catalyzing a diterpene biosynthesis precursor GGPP to synthesize framework precursor compounds harzianol I and wickeranol A of trichoderma specific diterpenoid trichoderma harzianum and trichodermin; in addition, the TriDTCs and the harzianol I have the biological functions of regulating and controlling the generation of trichoderma fungus chlamydospore and aspergillus oryzae nucleus. According to the invention, an efficient biosynthesis method is provided for heterologous synthesis of harzianol I and wickerol A, and biological and chemical ways are provided for enhancing generation of resistant spores of trichoderma and aspergillus oryzae.

The invention relates to a method for artificially cultivating biological soil crust, and belongs to the technical field of biological soil crust cultivation, and the method for artificially cultivating the biological soil crust comprises the following steps: collecting a biological crust seed source and artificially cultivating; the biological crust seed source is collected from a sand stabilization shrub area of the Maouu sand land, and the biological crust seed source comprises algae-containing crust, lichen crust and moss crust. According to the method, the collected biological crust seed sources are subjected to refined layering treatment, 1.5 cm of the upper layer is selected for preparing crust seed powder and reserving propagules, 1.5 cm of the lower layer is selected for preparing culture soil, substrates and nutrients are provided for the crust seed powder, and the collection area of the biological crust seed sources is reduced; symbionts are formed by inoculating arbuscular mycorrhizal fungi, so that the moisture and nutrient absorption capacity of crust is greatly enhanced, and the drought resistance and the adaptability to barren sand are improved.

Provided is a plant treatment method with which it is possible to further promote the growth of a plant using a compound such as ergothioneine. This method for treating a plant comprises a step for treating a propagule of a plant with an active ingredient which is a compound represented by formula (I), a tautomer thereof, or an agronomically acceptable salt thereof. [Chemical Formula 1] (In formula (I), R1 and R2 independently represent a hydrogen atom or an alkyl group having 1-4 carbon atoms, and R3, R4, and R5 independently represent an alkyl group having 1-4 carbon atoms)

Disclosed in the present invention are an auxiliary planting method and a system thereof, which aim at solving the technical problems of low survival rate, poor stability and non-standardized operation of planting in existing phytoremediation. A core method of the present invention comprises: a) preparing a plant propagule, wherein the propagule is a seed or a seedling; and b) deploying a structure, involving: deploying, in a region to be remediated, at least one auxiliary planting structure made of a biodegradable material, wherein the auxiliary planting structure is provided with at least one planting hole which can accommodate the propagule therein, and fixing the auxiliary planting structure to said region by using an anchor. The structure can provide physical support, water flow buffering, microenvironment improvement and biological protection for the propagule in the early stage of planting, and then degrades naturally. Further disclosed in the present invention is a system for implementing the method, which system comprises the auxiliary planting structure and the anchor. By means of the present invention, the standardization and engineering of phytoremediation operations for mangroves and salt marshes can be achieved, thereby enhancing the adaptability to complex environments. Moreover, the present invention is ecologically friendly, and has significant ecological and economic benefits.

The present disclosure relates to a method, a computer program product, and a system for creating pockets in a substrate material for planting plant propagules. The method (100) comprises determining (110) pocket parameters; controlling (120) relative position of an electromagnetic radiation source (310) and the substrate material (210); and cutting (130) a pocket (211) in the substrate material (210) based on the determined pocket parameters utilizing an electromagnetic radiation source (310).

The invention discloses an enteromorpha microcosmic propagule identification method and system based on a hologram and image processing, and the method comprises the steps: obtaining a digital hologram of an original mixed sample, and controlling the sampling rate of an observation algae solution through a negative feedback regulation mechanism; restoring the amplitude and phase distribution of the original mixed sample in the digital hologram by adopting an angular spectrum reconstruction algorithm to obtain a reconstructed amplitude diagram and a reconstructed phase diagram, calculating morphological parameters and optical characteristic parameters of the original mixed sample, and constructing a multi-dimensional characteristic vector; and inputting the multi-dimensional feature vector into a pre-trained recognition model, and outputting a recognition result. According to the method, efficient and accurate enteromorpha microcosmic propagule recognition is realized, and technical support is provided for detection and prevention of green tide pollution of water.

The invention discloses a method for comprehensively removing and treating invasive plant spartina alterniflora, and belongs to the technical field of ecological environment engineering. Comprising the following steps: in the growth period of spartina alterniflora, spraying a herbicide to stems and leaves; after 7-10 days of chemical treatment, plowing is conducted twice in the longitudinal direction and the transverse direction, the plowing depth is 35-60 cm, and exposure to the sun is conducted for 5-20 days after plowing; from the flowering stage of the spartina alterniflora to the early booting stage in the next year, the regenerated plants are cut till the stubble height is smaller than or equal to 10 cm. According to the method, chemical, physical and biological means are combined, overground and underground propagules are efficiently removed, the recurrence rate is reduced, and ecological restoration and environmental protection are both considered.

The invention relates to the field of plant cultivation, and provides a green seedling raising method for promoting rapid recovery of ecological vegetation after a fire, which comprises the following steps: S1, preparing a treatment liquid: adding an auxiliary agent into a carbonated beverage, and uniformly mixing to form the treatment liquid; s2, treatment: soaking a plant propagule in the treatment liquid; s3, flushing: flushing the plant propagules treated in S2 with clear water; s4, cultivation: planting the plant propagules washed in S3 in a culture medium for cultivation; according to the seedling raising method, the carbonated beverage is used as a main substrate and has a synergistic effect with a water-retaining agent, an acid-base regulator and a nutrition additive, so that the problems of phytotoxicity, environmental pollution and soil microbial community damage possibly caused by traditional chemical sterilizing agents and high-concentration hormones are effectively avoided, plant seed germination is promoted rapidly, the vegetation recovery period is effectively shortened, and the seedling raising efficiency is improved. And the method is more suitable for vegetation recovery after fire.

An apparatus for forming a rope matrix having seaweed propagules embedded therein is provided and may include an intertwining assembly configured to intertwine at least three ropes at a braiding point to form a rope matrix. Further, the apparatus may include a conveying device configured to introduce seaweed propagules adjacent to the braiding point and to enable embedding of the seaweed propagules within the rope matrix while the rope matrix is being formed. Further, the apparatus may comprises an offtake assembly configured to move the rope matrix, with the seaweed propagules embedded therein, out of the apparatus.

The application relates to the technical field of ecological shorelines, and particularly discloses a finger-shaped ecological shoreline for building microhabitats of aquatic organisms and a construction method thereof, which comprises a main revetment and a main water channel, a plurality of finger-shaped revetments are arranged at intervals on the side of the main revetment close to the water area, the end of the finger-shaped revetment away from the main revetment is inclined to the water flow direction, and the main revetment and the finger-shaped revetment are both provided with a plant community area; a water weir extending in the vertical direction is arranged between the end portions of the two adjacent finger-shaped revetments, the water weir is always located below the water surface, and a driving mechanism for driving the water weir to extend and retract is arranged in the water weir. The application increases the habitat diversity through the shaping of the finger-shaped revetment, and meanwhile, the water weir is lifted in the wet season to reduce the loss of aquatic plant seeds and propagules, animal eggs and larvae, and sediments; the water weir is lowered in the dry season, so that the organisms can communicate and inhabit between the water bay and the main water channel, and the biodiversity is improved.

The invention discloses an application of strigolactone in promoting symbiosis of arbuscular mycorrhizal fungi, and relates to the field of agricultural microorganisms, and an effective amount of strigolactone or a derivative thereof is combined with at least one arbuscular mycorrhizal fungi propagule for use; wherein the use concentration of the striga asiatica lactone is 0.01 nM to 10 M; according to the application of strigolactone in promoting symbiosis of arbuscular mycorrhizal fungi, through a Tween-20-containing solution carrier and a combined application mode, the activity of strigolactone is effectively protected, the action time of strigolactone is prolonged, the defect that strigolactone is easy to degrade is overcome, and the stability and repeatability of a field application effect are guaranteed.

A method of revegetating a native plant community such as a forest includes the steps of mechanically collecting forest floor material, specifically targeting native plant seeds, spores, and acrotelm layers, to restore native forest species. The collected material is formed into small pieces, such as pucks, for distribution on the target land. The pieces are formed by wetting the material with water and a binder as a wetting agent, mechanically compressed and dried to a moisture content less than 15% by volume. Each piece has a surface area of less than 25 sq cms and a thickness of less than 1.5 cms so that the pieces are smaller than the smallest length required for a root to emerge into a new plant. The process ensures that the material cannot dry out as the vegetative propagules die if it does. The material is formed into the pieces without being stockpiled or previously stored and without freezing.

The invention belongs to the technical field of biology. More precisely, a gel-like nutritional substrate containing at least meta-topoforest and melatonin and a method for producing the same are described. Also described is an artificial seed comprising a container filled with a nutritional substrate comprising at least melatonin and an intertopological forest in which a plant propagule is placed. The present invention also describes a method of in vitro culturing a plant in which the in vitro cultured meristem and / or embryonic tissue is placed on a nutritional substrate comprising at least meta-topoforest and melatonin, which later is exposed to stress conditions common in the field, enabling the propagule to grow into an adult plant. Preferably, the plant meristem and / or embryogenic tissue used is from a plant species that can be asexually propagated.

This process yields totipotent plant cells capable of regenerating plantlets, similar to propagation, in vitro micropropagation, and synthetic seed production. It leverages spray drying as a technology for preserving plant germplasm and as a novel method not currently available. The process begins with somatic cells in suspension and involves inducing friable embryogenic calluses from seeds, leaves, stems, roots, or any plant explant capable of generating cells in suspension, followed by obtaining the cell pack. This cell pack is mixed with encapsulating agents and then spray-dried. Finally, the spray-dried cells are rehydrated and reactivated to continue their morphogenic development, resulting in plantlets capable of acclimatizing to greenhouse and field environments.