418 results about "Mycorrhizal fungi" patented technology

Compositions, methods and business applications of using new and recycled cardboard infused with a plurality of saprophytic (including endophytic) and mycorrhizal fungi matched with seeds of plants (including trees, vegetables, herbs and grasses) whereby the cardboard can be sprouted by end-users to start ecosystems. Such containers may have carbon-credit value for companies and consumers when planted and grown as a carbon sink or carbon offset for the photosynthetic and mycelial sequestration of carbon dioxide. The relative weight of the Life Box's added seeds and spores does not significantly affect the total weight of the infused cardboard, thus not increasing transportation costs.

The invention relates to an application method of arbuscular mycorrhizal fungus in large-scale tobacco cultivation. A primary arbuscular mycorrhizal fungi fungicide is inoculated and propagated separately, and propagule spore density of an arbuscular mycorrhiza of each fungi after the propagation is 30 pieces per gram of dry soil. A float breeding method is adopted to conduct arbuscular mycorrhizal fungi fungicide combinedinoculation to tobacco, mycorrhization tobacco seedlings are obtained, and the mycorrhization tobacco seedlings are directly applied to field production. When being transplanted, the mycorrhization tobacco seedlings are conducted with a two-step inoculation, and the mixed fungicide is mixed by propagated acaulospora mellea, glomus mosseae and glomus intraradices at a proportion of 0.5-1: 1:1. A colony formed by the propagation of the arbuscular mycorrhizal fungus is close to a natural state, and fungus cooperate and play a better role of growth promoting and quality improving. According to the application method of the arbuscular mycorrhizal fungus in the large-scale tobacco cultivation, by means of the two times of inoculations of the arbuscular mycorrhizal fungus, the proportion of arbuscular mycorrhizal fungus and the tobacco in a symbiotic system built during a seedling stage of the tobacco is guaranteed, and the beneficial effects of the symbiotic system to the development of the tobacco seedlings are ensured. The application method of the arbuscular mycorrhizal fungus in the large-scale tobacco cultivation is simple and practical in method and environment friendly.

The invention relates to a microbial agent used for producing a soil modifying agent by fermentation, consisting of fiber monad, bacillus, lactobacillus, methanobacteria, thiobacillus, streptomycete, high-temperature actinomyces, high-temperature monad, azotobacteria, nitration monad, nitration bacilus, rhizobium, koji mold, leaven, blue mold, detritus mold, rhizopus as well as mycorrhizal fungi and substrate. In addition, the invention also relates to a soil modifying agent produced by the fermentation of the microbial agent, application thereof and the like.

The invention relates to a combined restoring method of polycyclic aromatic hydrocarbon contaminated soil, comprising the following restoring steps of: planting lucerne as perennial leguminous plants in the polycyclic aromatic hydrocarbon contaminated soil; inoculating a mixed bacterial inoculum comprising arbuscular mycorrhizal fungi, bacillus and flavobacterium; and meanwhile, adding rhamnolipid as a biosurfactant. By rhizosphere effect generated by plant root exudates, the number and the metabolic activity of soil microorganisms are increased; and by inoculating the arbuscular mycorrhizal fungi, the plant growth is promoted, and the root exudates are increased, thereby increasing the root accumulation of organic pollutants and the number of root soil microorganisms. The inoculated high-efficiency polycyclic aromatic hydrocarbon degrading bacteria use plant root secretions as a carbon source and an energy source and can quickly grow and propagate. The polycyclic aromatic hydrocarbon is stripped from soil grains by the biosurfactant, and the bioavailability and the degradability of the organic pollutants are increased.

The invention discloses a method for biologically restoring organic phosphorus pesticide polluted soil. An arbuscular mycorrhizal fungi and plant symbiotic system is used for accelerating the recovery of the organic phosphorus pesticide polluted soil. The method comprises the following steps of: (1) preparing an arbuscular mycorrhizal fungi agent; (2) inoculating the fungi agent to the soil to be restored, and planting quick growth plants, or transplanting the plants after mycorrhizal seedling culture; and (3) managing the plants to grow 2 to 3 months. The arbuscular mycorrhizal fungi agent comprises Glomus caledonium 90036, Acaulospora mellea ZZ, Glomus mosseae BEG167 and Glomus intraradices BEG 141). The host plant is selected from quick growth plants such as Sudan grass, alfalfa, corn, broomcorn and the like. The method can reduce the pesticide residue in agricultural products, reduce environment transfer to water, atmosphere and the like and reduce harm to human health, and is simple, convenient and environment-friendly.

The invention provides an application of arbuscular mycorrhizal fungi (AMF) in enhancement of growth and capability of heavy metal absorption from soil of black nightshade and a method of enhancing cadmium absorption of black nightshade from soil by utilization of the arbuscular mycorrhizal fungi. A cadmium (Cd) hyperaccumulating plant black nightshade is adopted as an experiment plant, the AMF is adopted as an Inoculant, the Cd hyperaccumulating plant black nightshade and the AMF capable of promoting plant growth are combined, and the AMF can enhance growth and capability of cadmium absorption from soil of the black nightshade, thus increasing the remediation efficiency of the black nightshade for Cd-contaminated soil. Compared with traditional chemical and agricultural measures, the method has characteristics of good treatment effects, no secondary pollution, low operation cost, and the like, and has good theoretical and application popularization value.

The invention is suitable for the technical field of recycling organic wastes, and discloses a flora for recycling treatment of organic wastes. The flora for recycling treatment of organic wastes is efficiently compounded from a variety of bacteria such as bacillus subtilis, aspergillus niger, saccharomycete and soil flora, wherein the soil flora is extracted from the natural world and comprises three or more of bacillus megatherium, bacillus mucilaginosus, bacillus licheniformis, bacillus thuringiensis, bacillus laterosporus, bacillus mucilaginosus, streptomyces jingyangensis, mycorrhiza fungi, azotobacter vinelandii, photosynthetic bacteria, bacillus coagulans, aspergillus oryzae, and paecilomyces lilacinus. According to the flora for recycling treatment of organic wastes, disclosed by the invention, by virtue of mutual promotion, mutual coordination and combined action of a variety of beneficial microorganisms, the organic wastes are recycled, environmental pollution is reduced, and wastes are changed into valuables; and the flora for recycling treatment of organic wastes can be used for producing industrial fermentation products and improving the health and growth conditions of animals, and can also improve the current situation of serious soil hardening at present and improve soil quality, thereby promoting the rapid development of agricultural economics.

The invention relates to a compound soil remediation agent containing microorganism bacteria and a preparation method thereof. The remediation agent comprises the following components in parts by weight: 0.01-3 parts of bacillus pasteurii spore powder, 5-50 parts of phosphogypsum powder, 10-60 parts of organic compost, 5-50 parts of turf powder, 1.5-5 parts of potassium fulvate powder, 0.2-0.5 part of powdery bacteria powder carriers and 0.15-1 part of glycerin. The remediation agent has round and smooth grains and high grain hardness, is harmless to seeds, can serve as a base fertilizer together with other fertilizers, can be sown together with seeds, integrates multiple remediation functions including organic complexation and passivation remediation, inorganic passivation remediation, microorganism passivation remediation and the like, has a comprehensive and strong soil remediation function, and can stimulate growth and reproduction of arbuscular mycorrhizal fungi at the roots of crops, accelerate heavy metal passivation, stimulate crop growth and enhance the immunities of crops.

The invention discloses a reproduction method of Chinese cymbidium seedlings, belonging to an orchid seedling reproduction technology, in particular to a reproduction technology for raising seedlings by tissue culture and mycorrhizal fungi intergrowth culture. In the reproduction method of the Chinese cymbidium seedlings, seedling acquisition mainly comprises the steps of seed germination, protocorm successive proliferation and rhizogenesis culture. The reproduction method of the Chinese cymbidium seedlings is characterized by comprising the following steps: carrying out hardening on rooted seedlings and the process of mycorhiza on mycorhiza fungi after the seedlings are obtained. By adopting the mycorhiza culture technology of sterile germination and mycorhiza fungi intergrowth of Chinese cymbidium seeds, the invention has the advantages that excellent variety can be bred, excellent Chinese cymbidium plants can be selected from the seedlings, the Chinese cymbidium breeding speed can be rapidly improved and the defects that pure tissue culture seedlings grow slowly, have long period and even do not bloom can be overcome, thus the invention has an important utilization value on large-area industrial reproduction for the Chinese cymbidium.

The invention discloses a novel industrial breeding matrix, which comprises the following components in percentage by weight: 13.8 to 14.2 percent of mycorrhizal fungi, 66 to 69 percent of turf, 3.4 to 4.6 percent of amino acid organic fertilizer, 6.9 to 8.1 percent of pearlite, 6.5 to 7.5 percent of vermiculite, and 0.25 to 0.48 percent of trianum. The mycorrhizal fungi, the trianum and the amino acid organic fertilizer are added into the traditional matrix, so that the breeding matrix can better promote crops to absorb nutrient elements, such as water, phosphorus, potassium, Zn, Cu and the like, and cultures high quality strong seedlings without germs and poisons, and has the advantages of high livability of the seedlings, short seedling stage of the seedlings, greater economic benefit, capability of saving cost of the seedlings and seeds.

The present invention utilizes fungal spore mass or hyphal fragments in landscaping cloths, fiber substrates, paper products, hydroseeders and agricultural equipment. The fungi may include saprophytic fungi, including gourmet and medicinal mushrooms, mycorrhizal fungi, entomopathogenic fungi, parasitic fungi and fungi imperfecti. The fungi function as keystone species, delivering benefits to both the microsphere and biosphere. Such fungal delivery systems are useful for purposes including ecological rehabilitation and restoration, preservation and improvement of habitats, bioremediation of toxic wastes and polluted sites, filtration of agricultural, mine and urban runoff, improvement of agricultural yields and control of biological organisms. The invention allow for a variety of methods and products including the use of cardboard boxes as a delivery system for fungi with or without the combination with plant seeds for starting gardens, for controlling insects, or for the process of ecological recovery.

The invention relates to a method for producing protective vegetable fungus root sprout, comprising the following steps: 1) planting susceptive crop after inoculating the bush AMF for sterilized organic soil or soil, taking the strand roots of plant or fungus root spore as inoculating bacteria; 2) sterilizing seedling soil or medium; 3) loading the sterilized soil into dish, spraying the inoculating bacteria onto soil, 4) sowing on the dish, then covering with sterilized soil for seedling in greenhouse. The invention is characterized by the low cost, good popularity and simple operation. The bacteria can on one side increase the usage of nutrient and water and fertilizer under organic cultivating condition, which results in productivity and quality improvement of vegetable; on the other side can strengthen the disease and drought resistance, reduce disease spread through soil and improve vegetable root condition.

The invention relates to a blueberry mycorrhizal fungi (coprinus micaceus) and a separation method and application thereof, belongs to the technical field of microbiology, and mainly relates to a mycorrhizal fungi which separated from a Changbai Mountain wild cowberry root system for blueberry inoculation and application of the mycorrhizal fungi at promoting the growth of blueberry and improving the yield of the blueberry. The blueberry mycorrhizal fungi is preserved in the Chinese General Microbiological Culture Collection Center, and the classification name is Coprinus micaceus. By the application of the mycorrhizal fungi, the growth of the blueberry root system can be promoted. The invention has the advantages that the yield, fruit setting rate and insect damage prevention function can be improved, and the like.

The invention discloses a method for improving the phytoremediation efficiency of low-concentration arsenic-contaminated soil, which comprises the steps of: planting corns in an arsenic-contaminated farmland, inoculating arbuscular mycorrhizal fungi and earthworms, pulling up the corns with roots after the growth season of the corn is over, harvesting the corns wholly, and performing centralized treatment. The method fully exploits the potentials of arsenic remediation of plants, microorganisms and animals, and inoculates the AM fungi and the earthworms to promote the plant absorption and dissipation of arsenic by promoting the growth of the plants, improving the activity of phosphatase of the soil and activating the arsenic in the soil to further achieve the aim of improving the phytoremediation efficiency of the low-concentration arsenic-contaminated soil. The method combines the microorganisms, the animals and the plants together for the in-situ remediation of the arsenic-contaminated farmland, has simple and convenient operation and friendly operating environment, cannot cause secondary pollution, reduces the treatment cost, and has good environmental effect.

The invention provides a method for locally culturing and producing arbuscular mycorrhiza fungus biological agent fertilizer in farmlands. The method is characterized by comprising the following steps of: 1, preparing a farmland culturing pond and a culture medium; 2, transplanting and culturing arbuscular mycorrhiza fungi; 3, absorbing and converting biological fertilizer; and 4, collecting biological fertilizer. Compared with the prior art, the technique adopted in the method has the advantages that: 1, the raw materials are readily available, the culture of the raw materials is easy, and the cost is low; 2, the culture technique and the production technique are conveniently popularized in rural areas; and 3, the production technique is green and environment friendly, which facilitates the development of modern agriculture. In addition, by using the fertilizer of the invention, the used amount of the quick-acting fertilizer is reduced and the used amount of partial fertilizer is reduced, so that the pollution to soil, water resources and air is lowered, and the fertilizer is particularly suitable to be used for planting seedlings of farm crops and medicinal plants. Tests show that the yield of the farm crops cultured by using the fertilizer of the method is improved by over 50 percent compared with that of the farm crops cultured by using the common fertilizer.

The invention discloses a multiple microbial manure applied in summer to increase quality of black tea. The microbial manure is fermented from the following raw materials by weight: amino selenium or / and amino zinc, a water-soluble carbon source and nitrogen source, and a mixed microbial solution of Bacillus subtilis and VA mycorrhizal fungi. According to analysis, the multiple microbial manure contains a large amount of enzymes (alpha-amylase, neutral protease) required for physiological and biochemical reactions during growth and development of tea tree, biotin, interferon, amino acids and other factors beneficial for life activity. These factors ensure strong growth of tea plants in summer, and resist disease, insect and drought, and realize strong root absorption function, high photosynthetic efficiency, UV resistance, resistance to formation and accumulation of anthocyanin in fresh leaves, so as to improve the quality of black tea.

The invention discloses separation of arbuscular mycorrhizal fungi as well as preparation and application of arbuscular mycorrhizal fungi fungicide, and belongs to the technical field of biology. The arbuscular mycorrhizal fungi is arbuscular mycorrhizal fungi rhizophagus irregularis which is called arbuscular mycorrhizal fungi rhizophagus irregularis CD-1 and is separated from soils; a specific sequence of verifying ribosome 28S rDNA is shown in SEQ ID NO.1; by utilizing the fungicide prepared by the fungi, the height of strains, the number of petioles, the overground fresh weight, the overground dry weight, the root fresh weight and the root dry weight of tomatoes can be increased, and the mycorrhizal infection rate delta gt can be obviously prompted; 80 percent of corns are high in selfing line strains and heavy in overground fresh weight and overground dry weight; the resistance to banded sclerotial blight by the corns is improved, and the arbuscular mycorrhizal fungi has an extremely-high genetic use value.

The invention relates to a method for producing dendrobium officinale tissue culture mycorrhizal seedlings, which comprises the following steps of: (1) culturing mycorrhizal fungi by using a solid or liquid culture medium, wherein the mycorrhizal fungi are mycorrhizal fungi LP1, namely Chaetomium sp.; (2) culturing mycorrhizal bacteria, wherein the mycorrhizal bacteria DP6 are Azotobacter sp.; (3) culturing the dendrobium officinale tissue culture seedlings; and (4) carrying out water culturing and training on the tissue culture seedlings. With the method for producing the dendrobium officinale tissue culture mycorrhizal seedlings, provided by the invention, not only the growth of the dendrobium officinale tissue culture seedlings is promoted, but also the tissue culture seedlings are obviously thickened. After transplantation, the survival rate can reach more than 95%, and the tissue culture seedlings have high growth rate, great bud tillering rate, rapid growth rate and strong stress resistance. Compared with the control group of dendrobium officinale seedlings which are not inoculated to mycorrhizal fungi and bacteria to perform tissue culture, the dendrobium officinale seedlings inoculated to mycorrhizal fungi and bacteria have more prosperous root systems, obviously inflated plant internodes and high fresh weight increased by 10-20%.

The invention relates to a method for improving artificial breeding survival rate of populus diversifolia by inoculating arbuscular mycorrhizal fungi. The method comprises the steps of: selecting populus diversifolia sprouts for performing pot experiment, arranging a transplanting hole in the pot, spreading sandy soils rich in glomus mosseae spores at the bottom of the hole, slowly rotating the root systems of populus diversifolia seedlings on the sandy soils of glomus mosseae spores before the transplantation, so that the sandy soils are pasted on the root systems of the populus diversifolia seedlings, transplanting the populus diversifolia seedlings into the hole in the pot, watering a dilute Hoagland nutrient solution every time, and observing the survival rate; or enabling populus diversifolia seeds to absorb water and swell and accelerating germination in an incubator, mixing the populus diversifolia seeds with the sandy soils of glomus mosseae spores, spreading the mixture on a sandy layer surface of a culture bed, and paving a layer of sandy soils rich in glomus mosseae spores under the sandy layer of the culture bed in advance, wherein the root systems are to pass through the sandy soil layer rich in glomus mosseae spores, so that the root systems can be infected. Compared with a control group, the populus diversifolia seedlings obtained by the method are obviously different from the populus diversifolia seedlings in the control group in the growing speed and survival time, particularly in survival rate.