The invention relates to an application method of
arbuscular mycorrhizal fungus in large-scale tobacco cultivation. A primary
arbuscular mycorrhizal fungi
fungicide is inoculated and propagated separately, and
propagule spore density of an
arbuscular mycorrhiza of each fungi after the propagation is 30 pieces per
gram of
dry soil. A float breeding method is adopted to conduct
arbuscular mycorrhizal fungi
fungicide combinedinoculation to tobacco, mycorrhization tobacco seedlings are obtained, and the mycorrhization tobacco seedlings are directly applied to field production. When being transplanted, the mycorrhization tobacco seedlings are conducted with a two-step
inoculation, and the mixed
fungicide is mixed by propagated acaulospora mellea, glomus mosseae and
glomus intraradices at a proportion of 0.5-1: 1:1. A colony formed by the propagation of the arbuscular mycorrhizal
fungus is close to a
natural state, and
fungus cooperate and play a better role of
growth promoting and quality improving. According to the application method of the arbuscular mycorrhizal fungus in the large-scale tobacco cultivation, by means of the two times of inoculations of the arbuscular mycorrhizal fungus, the proportion of arbuscular mycorrhizal fungus and the tobacco in a symbiotic
system built during a
seedling stage of the tobacco is guaranteed, and the beneficial effects of the symbiotic
system to the development of the tobacco seedlings are ensured. The application method of the arbuscular mycorrhizal fungus in the large-scale tobacco cultivation is simple and practical in method and environment friendly.