38results about How to "Increased fresh weight" patented technology

The invention relates to the technical field of plant genetic engineering, in particular to an application of an alfalfa MsPL19 protein and a gene thereof in regulating and controlling the plant height, fresh weight or biological yield of alfalfa. The invention provides an MsPL19 protein, a gene and application thereof, which can obviously improve the plant height, the biological yield and the fresh weight of medicago sativa and further improve the quality of the medicago sativa. Meanwhile, it is found that the MsPL19 protein and gene can respond to low temperature, drought, salt, hormone and other pathways, the protein interacting with MsPL19 is obtained through immunoprecipitation-mass spectrometry analysis, corresponding molecular modules can be enriched, the molecular mechanism of alfalfa MsPL19 responding to the low-temperature environment is analyzed, new cold-resistant varieties can be bred for alpine regions, and the application prospect is broad. And a new breeding direction is provided for increasing the yield of alfalfa.

The invention relates to the technical field of biology, in particular to application of coding sites of soybean PRR5a and PRR5b genes in the aspect of regulating and controlling salt tolerance and application of a mutant material generated by the coding sites in the aspect of regulating and controlling the salt tolerance of soybeans. According to the invention, an effective target for editing soybean PRR5a and PRR5b genes is subjected to double-strand breakage under mediation of endonuclease Cas9, so that different allele mutants of PRR5a and PRR5b are obtained. By knocking out the PRR5a and PRR5b genes, a new strategy is provided for enhancing the salt tolerance of the soybeans, reliable means and materials are also provided for cultivating new varieties of the soybeans and improving the salt tolerance, and the method plays an important role in research and production of crops.

The invention belongs to the technical field of soil conservation, and particularly relates to a method for promoting plant growth and regulating soil nutrients. The invention discloses a method for regulating and controlling corn and tobacco growth and rhizosphere soil nutrients through combination of AMF and a planting mode. The method comprises the following steps: preparing an AMF-containing microbial inoculum by using M. mosseae (F. mosseae); cultivating flue-cured tobacco seedlings and corn seedlings; flue-cured tobacco seedlings and corn seedlings are jointly planted in an intercropping mode by utilizing an AMF-containing fungicide. The method can relieve continuous cropping obstacles of flue-cured tobaccos and promote growth of the flue-cured tobaccos and corn, and has a certain effect on soil conservation.

The content of IAA produced by the enterobacter ludwigii XL0404 can reach 18.94 mg / L, the content of ACC deaminase produced by the enterobacter ludwigii XL0404 is 484.48 U / L, and the enterobacter ludwigii XL0404 has multiple functions of dissolving phosphorus, dissolving potassium, fixing nitrogen, producing siderophore and the like, has an inhibition effect on main root and stem disease pathogenic bacteria such as phytophthora nicotianae, rhizoctonia solani, rhizoctonia tectorum, fusarium oxysporum, fusarium solani and the like, and has an inhibition rate of 41.20-55.88%; the germination rate of the tobacco seeds is increased through treatment of the strain XL0404, and the root length is remarkably promoted; the root activity, the whole plant fresh weight, the overground fresh weight, the root fresh weight, the maximum leaf length and the maximum leaf width of the tobacco seedlings treated by the strain XL0404 with the bacterium content of 1 * 10 < 7 > cfu / mL in a seedling culture substrate are obviously higher than those of the tobacco seedlings treated by contrast treatment, and the strain XL0404 can be used as plant growth-promoting rhizobacteria and has a good application prospect in research and development of microbial fertilizers and biological prevention and control of tobacco rhizome diseases.

The invention belongs to the technical field of vegetable planting, and particularly relates to a seedling treatment composition and application thereof in improving stress resistance of solanaceous vegetables and promoting growth of the solanaceous vegetables. According to the method, solanaceous vegetable seeds are soaked in the seed treatment liquid obtained by dissolving the seedling treatment composition in water, the soaked seeds are subjected to dark culture, and the seedling treatment liquid is sprayed in the solanaceous vegetable seedling stage, so that the low temperature resistance of solanaceous vegetables can be effectively improved; the vigor of solanaceous vegetable seeds and the quality of seedlings under the low-temperature condition are improved. The test result shows that the seedling emergence potential, the seedling emergence rate and the seedling emergence index of the solanaceous vegetable seeds under the low-temperature condition can be improved and the average seedling emergence time can be shortened by using the seedling treatment liquid and culturing mode, and meanwhile, the chlorophyll content, the plant height, the root length and the fresh weight of the solanaceous vegetable seedlings under the low-temperature condition can be improved.

The invention discloses application of N-acetyl-L-cysteine in improving drought tolerance of plants, and belongs to the technical field of plant cultivation. The invention provides the application of N-acetyl-L-cysteine (N-acetyl-L-cysteine, NAC) in the improvement of the drought tolerance of the brassica napus for the first time. Experiments prove that compared with a control group, the fresh weight, the dry weight, the root length and the chlorophyll content of rape seedlings subjected to foliage spraying of 150 mu M of N-acetyl-L-cysteine under the drought stress condition are improved, the water loss rate of leaves is reduced, and the drought stress tolerance of the rape is enhanced. Therefore, the drought stress tolerance of the rape treated by the N-acetyl-L-cysteine is obviously improved. Furthermore, an optimal application concentration of 150 mu M is obtained through experimental exploration, so that the N-acetyl-L-cysteine can be optimally utilized in improving the drought stress tolerance of the oilseed rape.

The present invention discloses a new use of ccdC gene, and belongs to the technical field of microbial genetic engineering. The new use of ccdC gene disclosed by the present invention is to improve the motility of strains, reduce the wrinkling of bacterial biofilms, destroy the stability of biofilm structures, promote bacterial dispersion, promote spore formation, inhibit the growth of Nicotiana benthamiana, and inhibit the secretion of indoleacetic acid by strains; the nucleotide sequence of the ccdC gene is shown in SEQ ID NO.1. The present invention also discloses a ccdC gene mutant strain SZZ06. Compared with the wild type FZB42, the ccdC gene mutant strain SZZ06 of the present invention has reduced motility of strains, greater wrinkling of biofilms, more stable structure of biofilms, slower bacterial dispersion, less spore formation, and promotes the secretion of indoleacetic acid by strains and promotes plant growth.

The invention discloses preparation and application of a biological carbon type phosphate fertilizer synergist suitable for flue-cured tobacco, and relates to the technical field of phosphate fertilizer synergists. Comprising the following steps: step 1, preparing raw materials; 2, raw material treatment; and step 3, mixing and reacting: by taking the charcoal and the humic acid as main raw materials, the content of available phosphorus in soil can be increased, absorption and utilization of tobacco to calcium superphosphate and diammonium phosphate are increased, growth and development of the tobacco are promoted, the fresh weight of the tobacco leaves is increased, and the phosphorus accumulation amount of the tobacco is increased. According to the preparation and application of the biological carbon type phosphate fertilizer synergist suitable for flue-cured tobacco, the fixing agent, the water-retaining agent and the microbial agent are added into the biological carbon source to form the phosphate fertilizer synergist, the water-retaining agent can prevent water loss in soil, and the fixing agent can prevent phosphorus loss in the soil; and a mixture of compound sodium nitrophenolate and high-purity alpha-pimacol is added, so that the permeation, adhesion and spreading force of the fertilizer are increased, the limitation of plants is broken, and the capability of the fertilizer entering the plants is enhanced.

The invention relates to the technical field of microorganisms, and particularly provides bacillus amyloliquefaciens and application thereof in relieving salt stress of scleroderma plants. The preservation number of the bacillus amyloliquefaciens is CCTCC (China Center For Type Culture Collection) CNO: M 20242154; the nucleotide sequence of the bacillus amyloliquefaciens is as shown in SEQ ID NO. 1. Compared with the prior art, the bacillus amyloliquefaciens PG-4 provided by the invention can improve the osmotic regulation capability of the sclerodera plant seedlings under salt stress, enhance the tolerance to the salinized environment and promote the growth of the sclerodera plant seedlings under salt stress.

The invention discloses a preparation method of organic acid modified biochar as well as a product and application of the organic acid modified biochar, reference and a method are provided for resource utilization of a large number of waste grape branches, salicylic acid modified grape-based biochar obtained by modifying salicylic acid with the concentration of 50 mg / L and 500 mg / L has excellent specific surface area; therefore, excellent adsorption performance is obtained. According to the invention, the grape-based biochar obtained by modification with 500 mg / L of salicylic acid can improve the salt resistance level of eggplants under the stress of 100 mmol / L of NaCl. The invention provides a new method for efficient resource utilization of the rape straws. The rape straw biochar obtained by modifying the 100 mg / L ferulic acid can obviously improve the fresh weight of lettuce.

The invention discloses application of bentonite-loaded calcium-iron layered double hydroxides in passivating cadmium in tomato plants. The application comprises the following steps: increasing the fresh weight and dry weight of the tomato plants planted in cadmium-polluted soil; the plant height and root length of tomato plants planted in the cadmium-polluted soil are improved; and passivating cadmium in tomato plants planted in the cadmium-polluted soil. The bentonite-loaded calcium-iron layered double hydroxide is prepared by the following method: dissolving bentonite, FeCl3. 6H2O and CaCl2 in deionized water, adding Na2CO3 and NaOH solutions while stirring, adjusting the pH to 11-13 to obtain a solid-liquid mixture, filtering after reaction to obtain a precipitate, washing with deionized water to be neutral, drying and grinding to obtain the bentonite-loaded calcium-iron layered double hydroxide. The research aims at repairing Cd-polluted soil, bentonite and layered double hydroxides widely existing in the nature are used as raw materials, CaFe-LDH (at) BT is prepared by using a coprecipitation method, and the content of available Cd in the soil can be effectively reduced by applying 0.4% of CaFe-LDH (at) BT; the dry weight and fresh weight of tomato plants are improved, the content of Cd in roots, stems, leaves and fruits of the tomato plants is effectively reduced, and the transfer coefficient of Cd in the tomato plants and the enrichment coefficient of overground parts are also reduced.

The present invention relates to the technical field of ganoderma lucidum, and in particular to a ganoderma lucidum and its specific molecular marker and application. The present invention provides a hybrid strain ganoderma lucidum GZJ10, which has a sterile pore structure on the lower surface of the pileus, does not produce spore powder, has different properties from both parents, has a fast growth rate, high fresh weight of fruiting bodies, high biological conversion rate of fruiting bodies, and large flower shape. No spore powder is produced during the growth process, and it can be used as an excellent variety for cultivating ganoderma lucidum fruiting bodies. The present invention also provides an identification and verification method for hybrid strain specific molecular markers, and the identification and verification method is accurate.

The invention discloses application of medium-molecular-weight gamma-polyglutamic acid in reduction of heavy metal accumulation of crops and promotion of crop growth, the relative weight-average molecular weight range of the medium-molecular-weight gamma-polyglutamic acid is 200-300 kDa, and meanwhile, the invention provides a preparation method of the medium-molecular-weight gamma-polyglutamic acid. By adding the medium-molecular-weight gamma-polyglutamic acid into the soil, heavy metals Cr and Cu in the soil absorbed by the cucumbers can be remarkably reduced, the content of proline is remarkably increased, the total oxidation resistance is improved, the content of malondialdehyde is remarkably reduced, and the growth of the cucumbers is promoted. According to the method disclosed by the invention, the polyglutamic acid is degraded by adopting a one-step method (gamma-PGA) to obtain the medium-molecular-weight polyglutamic acid (gamma-PGA), so that absorption and enrichment of cucumbers on heavy metals Cr and Cu in soil can be reduced, the growth and development of the cucumbers can be remarkably promoted, and the fresh weight of fruits can be increased.

The invention provides bacillus stutzeri HMB39561 and application thereof, and belongs to the technical field of agricultural microorganisms. The bacillus stutzeri HMB39561 disclosed by the invention is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number of the bacillus stutzeri HMB39561 is CGMCC No.37015. The strain not only has a good prevention and treatment effect on cotton damping-off and verticillium wilt, but also can increase the cotton yield and improve the cotton quality. The bacillus stutzeri HMB39561 disclosed by the invention can be used for green prevention and control of cotton damping-off and verticillium wilt, and has a relatively good application prospect in the field of cotton planting.

The invention discloses a Chinese herbaceous peony 'Salaa' cut flower fresh-keeping method. The Chinese herbaceous peony 'Salaa' cut flower fresh-keeping method comprises the step of inserting Chinese herbaceous peony 'Salaa' cut flowers into fresh-keeping liquid containing metformin hydrochloride and acarbose. The metformin hydrochloride and the acarbose are especially added into the Chinese herbaceous peony cut flower fresh-keeping liquid, so that the effect of synergistically adjusting flowering genes of Chinese herbaceous peony can be achieved, the flowering phase of Chinese herbaceous peony, especially 'Saxa' fresh cut flowers, is practically and effectively prolonged, and the flower diameter, the flower height, the fresh weight, the flower fragrance and the like are improved.

The application discloses overexpression GmGAI The application belongs to the technical field of plant stress resistance and aims to improve the aluminum stress resistance of soybeans. The application provides an application of GmGAI protein in improving the aluminum stress resistance of soybeans, and the amino acid sequence of the GmGAI protein is shown as SEQ ID NO. 1. The application provides a theoretical basis for the aluminum stress resistance of soybeans.

The invention discloses a strain of Priesteria gigantea with carbon fixation function, a fermentation liquid and an application thereof, and belongs to the technical field of microbial application. The invention screens out a strain of Priesteria gigantea ( Priestia megaterium )C17, its biological preservation number is CCTCC NO: M 20241724; strain C17 has the carbon fixation gene cbbL gene, can produce carbon fixation enzymes, has good carbon fixation ability, can be used in the field of microbial carbon fixation, promote the conversion of inorganic carbon in the soil into organic carbon, and at the same time can improve the soil and increase the number of carbon fixation bacteria in the soil.

The present invention discloses a novel Paenibacillus sp. SN‑8‑1 and its application, and relates to the field of microbial technology. The novel Paenibacillus sp. SN‑8‑1 disclosed in the present invention is classified and named Paenibacillus sp. SN‑8‑1, which has been deposited in the China Microbial Culture Collection Center, with a deposit number of CGMCCNO.1.60135, a deposit date of January 16, 2024, and a deposit address of: Institute of Microbiology, Chinese Academy of Sciences, No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing. The novel Paenibacillus sp. SN‑8‑1 of the present application effectively promotes the increase of soybean plant height, the increase of fresh weight of the above-ground part of soybean, and the increase of fresh weight of the underground part of soybean; secretes organic acids and phosphatases; and can effectively dissolve insoluble inorganic phosphorus. It is used to solve the problem of low phosphorus utilization efficiency in agricultural and forestry land, and has good application and promotion value.

The invention discloses talaromyces pinophilus and application thereof. Belongs to the technical field of biological control. According to the invention, a strain HY25 capable of efficiently antagonizing seven kinds of tobacco fusarium is separated from rhizosphere of a diseased plant by a tissue isolation method and a plate confrontation method. The talaromyces pinophilus is identified as talaromyces pinophilus through morphological and molecular biological tree establishment. A further research shows that the strain HY25 has various growth-promoting and disease-preventing capacities of dissolving potassium, dissolving phosphorus, fixing nitrogen and the like. Pot experiment results show that the control effect of fermentation liquor of the strain HY25 on tobacco fusarium root rot is 75%, and the strain HY25 has a remarkable growth promoting effect on indexes such as plant height, root length, fresh weight and leaf surface area of tobacco. In conclusion, the strain HY25 has good biological prevention and control potential on the tobacco fusarium root rot, has a growth promoting effect, and enriches biocontrol strain resources of the tobacco fusarium root rot.

This invention provides a compound microbial agent, its preparation method, and its application in the control of plant root rot, belonging to the field of plant disease biological control technology. The compound microbial agent prepared by combining *Bacillus mohaiweiii* DS-L23, *Pseudomonas fluorescens* DS-PZ-L7, and *Pseudomonas aeruginosa* DS-L22 exhibits significant inhibitory effects against various plant pathogenic fungi of *Codonopsis pilosula*, including *Fusarium oxysporum*, *Fusarium solani*, *Fusarium trifidum*, *Pseudomonas heterostemum*, and *Alternaria alternata*. This compound microbial agent has advantages such as good stability and ease of cultivation. Furthermore, the construction method of the compound microbial agent is simple and easy for industrial mass production. After application, the compound microbial agent provided by this invention has a good control effect on *Codonopsis pilosula* root rot and can promote the growth of *Codonopsis pilosula* plants, reducing the use of chemical pesticides while improving the control effect.

The present invention belongs to the field of biological breeding technology, and specifically relates to the application of soybean malate dehydrogenase in regulating plant stress resistance. The technical problem to be solved by the present invention is: how to improve the stress resistance of soybean, for example, how to improve the salt tolerance of soybean. To solve this technical problem, the present invention provides the application of protein or a substance that regulates gene expression or a substance that regulates the activity or content of the protein in regulating plant salt tolerance, the gene encodes the protein, and the protein may be a GmMDH2 protein; the GmMDH2 protein is a protein whose amino acid sequence is shown in positions 1-352 of SEQ ID No.1. The results of the present invention show that GmMDH2 can significantly improve the salt resistance of transgenic plants, and GmMDH2 can be used as a salt-tolerant gene to cultivate soybean stress-resistant varieties.

The application relates to the technical field of agricultural production, and discloses a preparation method of licorice-wolfberry-based carbon quantum dots and application of the licorice-wolfberry-based carbon quantum dots in improving salt tolerance of crops as drip irrigation fertilizers. The licorice-wolfberry-based carbon quantum dots are synthesized by using wolfberry and licorice as main raw materials through a one-pot hydrothermal method; the synthesis method is simple; the solution after synthesis can be used after simple separation or filtration; and after the licorice-wolfberry-based carbon quantum dots are used for fertilizing crop roots, the fresh weight and SPAD value of crop seeds under salt stress can be effectively improved.

The invention provides Pseudomonas kirscheri WF3, a microbial fertilizer for saline-alkali soil and a preparation method and application of the microbial fertilizer, and belongs to the technical field of biological composting. The invention provides a preparation method of a microbial fertilizer for saline-alkali soil. The preparation method comprises the following steps: (1) preparing a fertilizer carrier comprising a decomposed organic fertilizer, humic acid, attapulgite powder and polyglutamic acid; and (2) inoculating pseudomonas kirschner WF3 into the fertilizer carrier, and carrying out sealed fermentation to obtain the microbial fertilizer for saline-alkali soil. Pseudomonas kirschner WF3 which has exopolysaccharide and organic acid production capacity and can survive in a strong saline-alkaline environment is adopted for fermentation of a fertilizer carrier, the prepared microbial organic fertilizer can effectively improve the content of organic carbon, total nitrogen and water-stable large aggregates (larger than 2.5 mm) in soil, increase the yield of corn, reduce the pH and total salt content of the soil, and improve the yield of corn. The improvement effect on the saline-alkali soil is obvious.

The invention relates to the technical field of low-grade phosphorite treatment, in particular to a medium-low-grade phosphorite acidolysis product as well as a preparation method and application thereof. According to the method, the raffinate acid is introduced to prepare the low-quality phosphate-based sulfur-phosphorus mixed acid urea to decompose the medium-low-grade phosphorite, so that the incremental utilization of the raffinate acid and the full-amount utilization of phosphorite resources are realized; the introduction of the raffinate acid creates good reaction conditions for the acidolysis process, is beneficial to relieving the generation of bubbles in the reaction process, reduces the foaming rate, and improves the conversion rate of elements in the medium-low grade phosphorite, and the highest conversion rate can reach 97.5% or above; and the fluidity of the acidolysis slurry is effectively improved by the raffinate acid, so that convenient conditions are provided for preparation of middle-low-grade phosphate ore acidolysis products.

The invention relates to the technical field of agricultural biology, in particular to application of a brassica napus BnaC05. BZIP68 gene to improvement of salt tolerance of plants. The sequence of the BnaC05.bZIP68 gene provided by the invention is as shown in SEQ ID NO.1, and the sequence of a protein coded by the BnaC05.bZIP68 gene is as shown in SEQ ID NO.2. The invention discovers that wild brassica napus can highly express the BnaC05.bZIP68 gene in a salt stress environment, and meanwhile, transgenic brassica napus overexpressed with the gene has stronger salt tolerance, so that the BnaC05.bZIP68 gene can be applied to the salt stress environment of the brassica napus. Therefore, the effect of the BnaC05. BZIP68 gene as a forward regulatory factor in enhancing the salt tolerance of the rape is verified, and a key molecular tool is provided for developing a new variety of brassica napus with high salt tolerance. The gene has wide application potential and market value in the field of agricultural planting, especially in improving the salt tolerance of plants and cultivating transgenic crops with high salt tolerance.

The invention belongs to the technical field of microorganisms, and discloses fusarium oxysporum, the strain of the fusarium oxysporum is HuN8, the taxonomic name is Fusarium oxysporum, the preservation unit is China General Microbiological Culture Collection Center (CGMCC), the preservation date is December 22, 2023, and the preservation number is CGMCC No.41016. The invention further discloses a preparation method of the fusarium oxysporum. The strain has a remarkable effect on prevention and treatment of wheat basal stem rot, can promote wheat growth, improve fresh weight and plant height, and also can enhance resistance of wheat to pathogenic bacteria such as fusarium graminearum.

The present invention discloses a forage growth promoter and the application of daidzein, genistein or palmitic acid in promoting forage growth. The active ingredient of the forage growth promoter is a combination of one or more of daidzein, genistein or palmitic acid. The three allelopathic substances extracted and separated from alfalfa, daidzein, genistein or palmitic acid, have a growth-promoting effect on alfalfa or ryegrass within a certain concentration range. The three components can be used as the effective ingredients of the forage growth promoter to promote the planting of high-quality forage such as alfalfa or ryegrass. In addition, at high concentrations, daidzein, genistein or palmitic acid, four natural substances present in plants, can be used as ecological herbicides to inhibit forage growth, which has the advantages of environmental protection and pollution-free.

The invention discloses bacillus velezensis ZKY08 for preventing composite infection of banana wilt and soft rot and application of the bacillus velezensis ZKY08. The Bacillus velezensis ZKY08 is preserved in the Guangdong Microbial Culture Collection Center (GDMCC) on July 8, 2024, the address is the fifth floor, the building 59, the No.100, the Xianlie Middle Road, the Xiaoxian District, Guangzhou City, Guangdong Province, the postal code is 510070, and the preservation number is GDMCC No: 64844. The bacillus velezensis ZKY08 disclosed by the invention can be used for effectively preventing and controlling composite diseases of banana soft rot and banana fusarium wilt, the prevention and control effect can reach 64.7%, and the bacillus velezensis ZKY08 has the effects of increasing the plant height, stem girth and overground fresh weight of bananas after being applied. The microbial agent can effectively promote banana growth while preventing and controlling diseases in a green manner, and is a biological prevention and control microbial agent with a good development prospect.

The invention relates to the field of agricultural biology, and particularly discloses a preparation method of phenylalanine (cyclo (leucine-phenylalanine) dipeptide) and application of the phenylalanine (cyclo (L-leucine-L-phenylalanine) dipeptide) in agriculture. The active ingredient of the composition comprises cyclo (L-leucine-L-phenylalanine) dipeptide Cyclo (-L-Leuu-L-Phe) obtained by fermenting and extracting external myrothecium advena CGMCC No. 42202; according to the invention, the cyclic (L-leucine-L-phenylalanine) dipeptide is scientifically compounded with different bioactive substances, and an application mode of flushing or spraying is adopted, so that the growth of crops can be effectively promoted, and the stress resistance of the crops can be enhanced. Specifically, on various crops such as corn, lettuce and tomato, the composition can significantly improve key growth indexes such as plant height, root length, stem diameter and fresh weight.

The invention relates to the field of microbial technology application, in particular to a bacillus methylotrophicus 9912 microbial inoculum and natamycin which are combined in a certain amount. The method is used for field planting of panax notoginseng, has an obvious prevention and treatment effect on panax notoginseng root rot, the prevention and treatment effect can reach 85% or above, growth of panax notoginseng can be promoted, and the fresh weight of underground roots of panax notoginseng plants and the content of notoginsenoside are obviously increased. The method is simple to operate, low in cost, easy to popularize, green and environment-friendly, and lays a good foundation for pollution-free planting of pseudo-ginseng.