24 results about "Membrane lipid peroxidation" patented technology

The invention discloses an application of a PpWRKY53 transcription factor in improving heat resistance of Chinese pear fruits, the transcription factor is a positive regulatory factor of Chinese pears responding to high temperature stress, overexpression of the transcription factor PpWRKY53 gene in the Chinese pear fruits can significantly reduce hydrogen peroxide accumulation and membrane lipid peroxidation caused by high temperature, effectively alleviate water stain type browning symptoms of the fruits, and improve the heat resistance of the Chinese pears. The fruit hardness is improved. The invention provides a key gene resource for creating new germplasm of heat-resistant Chinese pears and breeding new varieties with high-temperature-resistant characteristics, and has important scientific and application values for ensuring stable production of summer Chinese pear industry and improving fruit quality and economic benefits.

The application belongs to the technical field of genetic engineering, and particularly relates to a citrate synthase gene AtCS The application relates to the application of a citrate synthase gene in regulating the anti-aging ability of seeds and a method for screening seed anti-aging agents. AtCS The application provides a citrate synthase gene AtCS The nucleotide sequence of the citrate synthase gene is shown as SEQ ID NO. 1. The citrate synthase gene AtCS (referred to as AtCS gene) encodes a citrate synthase located in mitochondria. AtCS The gene plays a key role in regulating the active oxygen homeostasis and anti-aging ability of seeds. AtCS The germination rate of the seed of the gene knockout mutant significantly decreases after aging, membrane lipid peroxidation products accumulate, and the active oxygen scavenging system is damaged. The mutant can be used for screening seed anti-aging agents, and provides a new solution for seed anti-aging.

The invention relates to the technical field of plant genetic engineering and agricultural planting, in particular to application and method of an SlGRXC9 gene in improvement of tomato cadmium stress tolerance, the number of the SlGRXC9 gene is Solyc07g053550, the core coding region sequence of the SlGRXC9 gene is SEQ ID NO.1, and the coded protein sequence is SEQ ID NO.2; through construction of SlGRXC9 overexpression transgenic tomatoes, heterologous expression of protein encoded by the gene in plant cells or use of the gene as a molecular breeding target, the system activity of antioxidant enzymes such as SOD, POD, CAT and GR in tomatoes can be enhanced, cadmium stress induced H2O2 and O2-accumulation and membrane lipid peroxidation damage are reduced, and absorption and accumulation of the tomatoes to cadmium ions are not affected; according to the method, the growth ability and physiological stability of the tomatoes under cadmium stress are remarkably improved, the risk of nutrient imbalance is avoided, the hereditary stability is high, and the method can be widely applied to safe production of the tomatoes in cadmium-contaminated soil and creation of stress-tolerant germplasm.

This invention discloses a method for enhancing the high-temperature resistance of citrus through exogenous spraying of naringin, belonging to the field of agricultural technology. The method involves preparing naringin into a formulation and spraying it exogenously onto the leaves of citrus plants. Experiments show that after treatment with naringin, phenotypic damage such as wilting and curling of citrus seedling leaves under high-temperature stress is significantly alleviated; the maximum photochemical efficiency of photosystem II (Fv / Fm) is significantly increased; electrolyte leakage rate and malondialdehyde content are significantly reduced; and the accumulation of hydrogen peroxide and superoxide anions is effectively inhibited. This invention utilizes naturally sourced naringin to significantly enhance the tolerance of citrus to high-temperature stress through multiple mechanisms, including maintaining photosynthetic system function, protecting cell membrane integrity, inhibiting membrane lipid peroxidation, and scavenging reactive oxygen species. Furthermore, the method is simple to operate, environmentally friendly, and low-cost, making it suitable for stress-resistant citrus cultivation.

The invention discloses a coprophilous fungus capsule freeze-drying protective agent for intestinal flora transplantation and a preparation method of the coprophilous fungus capsule freeze-drying protective agent for intestinal flora transplantation and belongs to the technical field related to pharmaceutical preparations, and the coprophilous fungus capsule freeze-drying protective agent for intestinal flora transplantation comprises nano-liposome; polydextrose; plant selenoprotein; tea polyphenol; vitamin E; chitosan oligosaccharide; an Omega-3 fatty acid; 3-5% of inulin; the nano-liposome forms a phospholipid bilayer to form a physical barrier on the surface of a cell, and polydextrose and inulin regulate the extracellular medium viscosity and inhibit the growth rate of ice crystals; omega-3 fatty acid is inserted into a phospholipid bilayer to maintain the fluidity of a membrane at a low temperature, vitamin E and tea polyphenol reduce membrane lipid peroxidation products in the freeze-drying process, and the stability of an oxidant cell membrane is protected through damage of ice crystals, so that cells are protected, and the survival of the cells is improved.

This invention discloses the application of the cotton GhATHB-7 protein and its encoding gene in regulating plant salt tolerance. This invention is the first to identify the association between the cotton GhATHB-7 protein and its encoding gene and salt stress, and confirms that silencing this gene reduces the salt stress tolerance of cotton. This invention also discovers and demonstrates for the first time that the cotton GhATHB-7 protein enhances plant salt tolerance by increasing the membrane lipid peroxidation product MDA and reducing chlorophyll used for photosynthesis. The cotton GhATHB-7 protein and its encoding gene regulating plant salt tolerance in this invention have important value in enhancing plant salt tolerance and in breeding.

The invention discloses a method for preventing and treating fusarium oxysporum by using trans-cinnamic acid and palmitic acid, and belongs to the technical field of biological prevention and treatment of plant diseases. The bacillus subtilis B579 and the fusarium oxysporum (FOC) are co-cultured to induce bacteriostatic metabolites, key bacteriostatic metabolites including trans-cinnamic acid (CA) and palmitic acid (PA) are screened out through metabonomics analysis, the optimal bacteriostatic concentration of the key bacteriostatic metabolites is determined to be 50, and the bacteriostatic effect is achieved by destroying the completeness of cell membranes of pathogenic bacteria and initiating membrane lipid peroxidation. The invention further provides a biological agent containing CA and PA and an application method, greenhouse pot experiment verifies that the prevention and control effects on cucumber fusarium wilt respectively reach 67.58% (CA) and 47.29% (PA), the environmental compatibility is excellent, and an efficient and safe prevention and control scheme is provided for sustainable development of the facility cucumber industry.

The application of ceramide in the control of rice blast fungus falls within the field of biological control. This invention relates to the application of ceramide in the control of rice blast fungus: a combination of ceramide and lipopeptides is used for the control of rice blast fungus. Exogenous addition of ceramide significantly enhances the stress effect of lipopeptides on rice blast fungus. It also enhances the abnormal accumulation of glycogen and lipid droplets in conidia, promotes the bursting of ROS, and increases intracellular ceramide and calcium... 2+ The invention increases the content of malondialdehyde (MDA) in mycelium, enhances membrane lipid peroxidation, and decreases ergosterol content. Furthermore, it inhibits the expression of ergosterol synthesis genes, UPR target genes, and sphingolipid metabolism-related genes. This invention develops a highly efficient natural agent against rice blast by combining exogenous Cer with lipopeptides. This agent effectively reduces the pathogenicity of rice blast fungus and significantly inhibits its infection of rice, laying the foundation for the development of novel biological control agents.

The application belongs to the technical field of plant stress resistance regulation, and particularly relates to application of kynurenic acid in improving salt stress adaptability of cynodon dactylon. The application finds that, before and / or during salt stress, exogenous application of kynurenic acid to cynodon dactylon can significantly reduce the degree of oxidative stress (H2O2), the level of membrane lipid peroxidation (MDA) and the content of osmotic regulator substance proline, effectively relieve salt damage reaction and improve the adaptability of cynodon dactylon in a high-salt environment. The method is simple in operation, suitable for plant remediation and environmental protection planting in saline soil, and has a good application prospect.

The invention particularly relates to a 6-BA-coated MSNs nano composite material as well as a preparation method and application thereof, and belongs to the technical field of agricultural nano materials and crop stress resistance. The nano composite material is formed by loading 6-benzylamino adenine (6-BA) on mesoporous silica nano particles (MSNs), the average hydration particle size is 30-50 nm, the absolute value of Zeta potential is greater than 15 mV, and the nano composite material has a characteristic ultraviolet absorption peak at 260 nm. The preparation method comprises the following steps: mixing and stirring a 6-BA ethanol solution and an MSNs dispersion liquid, centrifugally washing and drying. The composite material can be used for preparing a seed initiator for relieving plant drought stress, can remarkably improve the seed germination rate under the drought stress, promote seedling growth, enhance antioxidant enzyme activity and relieve membrane lipid peroxidation damage through a dual synergistic mechanism of nano-carrier slow release and physical protection, has the effect superior to that of a single component, and has a wide application prospect. Good application prospects are realized.

The invention provides application of a tomato SlMT5 gene in improving high temperature stress resistance of plants, and belongs to the technical field of plant genetic engineering.Experiments show that under high temperature stress, an overexpressed plant containing the SlMT5 gene shows remarkably enhanced heat-resistant phenotype compared with a wild type, specifically, the withering degree and the heat damage index of the plant are remarkably reduced, and the heat-resistant phenotype of the overexpressed plant containing the SlMT5 gene is remarkably improved. The relative water content of the leaves is obviously increased. Meanwhile, the overexpressed plant can better maintain the photosynthesis capacity, and the maximum photochemical efficiency (Fv / Fm) and chlorophyll content of the overexpressed plant are remarkably higher than those of a wild type. In the aspect of oxidation resistance, the activity of antioxidant enzymes (SOD, POD and CAT) of overexpressed plants is remarkably enhanced, accumulation of active oxygen (and) and membrane lipid peroxidation products (MDA) is remarkably reduced, and the content of osmotic regulation substances (proline, ascorbic acid and soluble sugar) is remarkably increased; the invention provides key gene resources and technical support for cultivating new varieties of high-temperature-resistant crops.

The invention discloses a melatonin preparation for improving the salt stress resistance of carya illinoensis and a preparation method of the melatonin preparation, and relates to the technical field of biology. The melatonin preparation comprises a first active component, a second active component and auxiliary materials, the first active component is melatonin; and the second active component is tuberostemonine or dioscin. According to the invention, tuberostemonine and dioscin are introduced to respectively form a synergistic effect with melatonin, so that the salt stress resistance of the carya illinoensis can be remarkably improved. The tuberostemonine or the dioscin is combined with the melatonin, so that the activity of antioxidant enzymes such as superoxide dismutase and peroxidase can be activated, active oxygen can be effectively removed, and the content of malondialdehyde can be reduced, so that the membrane lipid peroxidation damage can be alleviated, and the cell membrane stability can be maintained; meanwhile, chlorophyll synthesis can be promoted, inhibition of salt stress on photosynthesis is relieved, seedling biomass accumulation is enhanced, the fresh weight is remarkably improved compared with that of salt stress control, and the comprehensive resistance to salt stress is shown.

Disclosed is the application of humic acid in improving the phytoremediation effect of heavy metals in water, and relates to the technical field of environmental ecological engineering. The invention discloses the application of humic acid in improving the phytoremediation effect of heavy metals in water, wherein the phytoremediation of heavy metals is to reduce the content of heavy metals in water by planting Vallisneria chinensis. Adding humic acid can slow down the chlorosis of Vallisneria vallissima leaves under heavy metal poisoning and increase the accumulation of heavy metals in Vallisneria vallissima leaves and roots. At the same time, it can also reduce the leaching ability of heavy metals in water, enhance the enzyme activities related to active oxygen metabolism in plants by stimulating the synthesis of protein and enzymes in various organs of plants, reduce the MDA concentration in plants, adjust the active oxygen content in plants, reduce the degree of membrane lipid peroxidation and enhance the resistance of plants to heavy metals.

The invention discloses application of a CeCHS57 gene in improving drought resistance of cyperus esculentus, and relates to the technical field of biology. The nucleotide sequence of the CeCHS57 gene is as shown in SEQ ID NO. 1. According to the invention, the CeCHS57 gene is successfully cloned from cyperus esculentus, so that the blank of research on the drought-resistant functional gene of cyperus esculentus is filled. After the gene is over-expressed in cyperus esculentus and arabidopsis thaliana through genetic transformation, the activity of antioxidant enzymes such as SOD, CAT, POD and APX in plant leaves and root systems can be remarkably enhanced, active oxygen accumulated under drought stress is effectively eliminated, the content and conductivity of membrane lipid peroxidation products MDA are reduced, and cell membrane damage is reduced, so that the tolerance of plants to drought stress is improved. Phenotypic analysis shows that the growth state of transgenic arabidopsis thaliana under drought conditions is obviously superior to that of wild and mutant plants, and the drought resistance function of the CeCHS57 gene is proved.

The invention discloses a synthesis method of monatomic manganese-doped lipophilic carbon quantum dots, and belongs to the technical field of biomedical materials, and the synthesis method specifically comprises the following steps: 1) adding manganese chloride into PEG400, and uniformly mixing to form a uniform mixed solution A; 2) adding p-phenylenediamine into the mixed solution A obtained in the step 1), and uniformly mixing to form a uniform mixed solution B; and 3) carrying out a hydrothermal reaction on the mixed solution B obtained in the step 2), after the reaction is completed, cooling to room temperature, washing, purifying and drying to obtain the monatomic manganese-doped lipophilic carbon quantum dots. Meanwhile, the invention further discloses that the Mn-coated Lip-CDs can specifically target a cytoplasmic membrane and remarkably inhibit lipid peroxidation of the cytoplasmic membrane, the oxidation resistance of the Mn-coated Lip-CDs is superior to that of a common lipid peroxidation inhibitor Trolox, and the Mn-coated Lip-CDs can be effectively used for treating ferroptosis.

The invention discloses synthesis of a novel plant growth regulator and application of the novel plant growth regulator in improvement of crop stress resistance, and relates to the field of agricultural biology. The composition is composed of the following components in parts by weight: 10-20 parts of sodium alginate oligosaccharide; 30 to 50 parts of a functionalized polydopamine nano carrier PDA (at) Ce; 5 to 10 parts of yeast source defense peptide YDP-125; the chelating auxiliary agent comprises 2-5 parts of naphthylacetic acid potassium salt and 3-8 parts of fulvic acid. The synergistic effect of all the components is utilized, the sodium alginate oligosaccharide is used for activating an ethylene signal channel and starting stress-resistant gene expression, the yeast source defense peptide YDP-12 is used for simulating phytophyton, biosynthesis of jasmonic acid is stimulated, protein accumulation of PR-2 and PR-3 is promoted, virus resistance is enhanced, PDA at Ce is chelated with nutrients through phenolic hydroxyl groups, ROS response type cerium ion release is achieved, Fenton reaction is catalyzed, and the effect of improving stress resistance is achieved. The. OH is converted into H2O2; chelated Zn < 2 + > and Mn < 2 + > enhance the SOD activity, so that the content of membrane lipid peroxidation markers is reduced; fulvic acid and sodium alginate oligosaccharide form a complex, DNA methylation modification is continuously induced, and stress resistance phenotype maintenance is promoted.

The invention relates to the technical field of microorganisms, and particularly provides application of trichoderma asperellum MM7 in constructing a drought-resistant banana rhizosphere microflora and improving the drought resistance of bananas. The trichoderma asperellum MM7 inoculation can relieve the inhibition of drought stress on the growth of banana seedlings and improve the water content and survival rate of tissues; the root antioxidant enzyme activity is enhanced, the accumulation of osmotic regulation substances is promoted, and the membrane lipid peroxidation degree is reduced; the expression of ABA metabolism and signal channel related genes and aquaporin genes is up-regulated; a rhizosphere bacterial community structure under drought stress is remodeled, beneficial functional bacteria such as pseudomonas, streptomyces and nitrococcus are remarkably enriched, and the drought resisting function of the bacillus amyloliquefaciens depends on indigenous microbial communities. The invention reveals that the trichoderma asperellum MM7 provides a new strain resource for developing a banana drought-resistant biological agent based on microbiome regulation through a synergistic interaction mechanism of directly regulating host physiology and gene expression-indirectly remodeling rhizosphere beneficial microflora.

The present application belongs to the technical field of vegetable storage and preservation, and particularly relates to a fresh vegetable storage and preservation method. The fresh vegetable is coated with pepper leaf essential oil and then packaged; the packaged vegetable is stored in a low-pressure electrostatic field and a low-temperature environment. The present application uses the pepper leaf essential oil coating, combined with the low-temperature and low-pressure electrostatic field preservation technologies, which have a synergistic effect, can obviously inhibit the pectin and cellulose degradation enzyme activity, reduce the metabolic decomposition of the cell wall structure components such as pectin and cellulose, reduce the loss of the nutritional components and water of the vegetable, maintain the texture characteristics of the vegetable, delay the oxidative decomposition of the chlorophyll components, thereby maintaining the original color quality of the vegetable, delaying the yellowing of the vegetable, inhibiting the postharvest physiological metabolism of the vegetable, delaying the consumption of the soluble solids of the vegetable, inhibiting the membrane lipid peroxidation, reducing the accumulation of malondialdehyde, and prolonging the shelf life of the fresh vegetable.

This invention relates to a method for promoting the germination of buckwheat seeds under cadmium stress and alleviating cadmium toxicity during the seedling stage. The method includes treating buckwheat seeds with a melatonin reagent before germination. The method involves contacting the plant seeds with a melatonin solution at a concentration of 100-500 μmol / L. The method provided by this invention can effectively improve the germination potential and germination rate of buckwheat seeds under cadmium stress, enabling uniform and rapid germination. This invention solves the important problem of how to ensure normal germination of buckwheat in cadmium-contaminated soil in agricultural production and has significant application value in production. This is likely because pretreatment with a melatonin reagent can induce the expression of antioxidant enzyme activity, scavenge the large number of free radicals generated by cadmium stress, alleviate oxidative stress, reduce oxidative damage, and maintain a low level of membrane lipid peroxidation in the seeds, thereby promoting normal seed germination.

The application discloses a method for improving the seedling rate of rice in waterlogging direct seeding, and utilizes Camellia sinensis endophytic actinomycete to treat rice seeds, wherein the Camellia sinensis endophytic actinomycete is Streptomyces sp. CoT10, and the strain preservation number is CGMCC No. 22968. In the method, the Camellia sinensis endophytic actinomycete Streptomyces sp. CoT10 can improve the enzyme activity of the antioxidant enzyme system (peroxide dismutase SOD, catalase CAT and antioxidant enzyme POD) of waterlogging rice, reduce the content of malondialdehyde, a membrane lipid peroxidation product, in the waterlogging rice plant, improve the waterlogging stress resistance of the rice, improve the seedling rate of the rice in waterlogging direct seeding, improve the chlorophyll content, plant height, root length and fresh and dry weight of the aboveground part and underground part of the rice under the condition of waterlogging direct seeding, and finally reduce the yield reduction loss of the rice caused by waterlogging.

The invention discloses application of a luteolin-zinc composite material in multi-target inhibition of valsa mali, and the luteolin-zinc composite material can cause the membrane potential change of a hypha cell membrane of the valsa mali to generate depolarization and destroy the integrity of the cell membrane to cause the leakage of intracellular nucleic acid and protein, so that the valsa mali is inhibited. An inhibiting effect and a killing effect are achieved on apple rot fungi; the ATP level in cells of valsa mali can be remarkably reduced, the activity of alkaline phosphatase is improved, and the content of malondialdehyde and the level of active oxygen are improved, so that bacteriostasis is realized through multiple ways of interfering energy metabolism, inducing membrane lipid peroxidation, oxidative stress and the like; in drug research and development and practical application, people can more scientifically and efficiently use the composite material to resist the apple rot fungi through the clear key concentration value, namely, the minimum inhibitory concentration of 4mM, and the composite material has important significance for guaranteeing healthy development of the apple industry and is expected to bring a new solution to prevention and treatment of the apple rot.

The invention discloses an edible mushroom fresh-keeping method based on modified atmosphere packaging cooperating with a static magnetic field, and belongs to the technical field of edible mushroom postharvest fresh-keeping, and the edible mushroom fresh-keeping method comprises the following steps: S1, pre-cooling the freshly harvested edible mushrooms; s2, putting the pre-cooled edible mushrooms into a packaging box, filling the packaging box with controlled atmosphere gas, and sealing the packaging box with a film; s3, static magnetic field treatment is applied to the edible mushrooms subjected to modified atmosphere packaging in the step S2; s4, the environment temperature is controlled to be 4 + / -1 DEG C in the whole process. According to the method, the postharvest quality, including sensory quality, physical quality and nutritional quality, of the stropharia rugoso-annulata can be comprehensively kept. The fresh-keeping mechanism is illuminated from the physiological and biochemical level, that is, the activity of antioxidant enzymes such as SOD, CAT, APX and the like is remarkably improved, reactive oxygen species (ROS) are effectively removed, the content of membrane lipid peroxidation products MDA is reduced, and the integrity of a cell membrane structure is maintained. At the same time, it is revealed from the molecular biology level that aging is delayed from the source by regulating and controlling gene expression of energy metabolism, an anti-oxidation system and related metabolic pathways.

The invention discloses a method for relieving plant tin (Sn < 4 + >) stress through calcium folate and application, and belongs to the technical field of plant stress resistance and heavy metal pollution remediation. Aiming at the problems of high cost, single target spot, insufficient metabolic regulation and the like caused by dependence on a chelating agent or an antioxidant in the prior art, multiple-effect synergistic alleviation is realized by exogenously applying calcium folate with a specific concentration: plant leaf shedding and root length shortening induced by Sn < 4 + > are inhibited, active oxygen (H2O2 and O2-) is effectively removed, membrane lipid peroxidation damage is reduced, and the yield of the plant is increased. The antioxidant enzyme activity and chlorophyll synthesis capability are recovered, and the photosynthetic function is improved. In a molecular mechanism, a folic acid synthesis pathway is activated and related gene expression is regulated and controlled. The method can synchronously improve plant stress resistance and heavy metal restoration efficiency, can reduce Sn < 4 + > bio-availability when applied to aquatic plants, can block heavy metal food chain transmission when applied to terrestrial crops, and provides an innovative technical scheme for ecological management and agricultural safety production.

The application discloses application of L-carnitine in improving cold tolerance of plants and belongs to the technical field of plant stress tolerance regulation and stress resistance agent application. The application utilizes L-carnitine to improve the PS II maximum quantum yield (Fv / Fm) of Arabidopsis thaliana, enhances the stability of the photosynthetic system, reduces the damage of low temperature to the photosynthetic mechanism, simultaneously reduces the content of malondialdehyde (MDA), inhibits membrane lipid peroxidation, maintains the integrity of the cell membrane, promotes the synthesis of proline and soluble sugar, improves the cell osmotic regulation capacity and strengthens the cold resistance. The application overcomes the defects of the existing genetic improvement, physical regulation and chemical regulation technologies, such as narrow application range, high cost and poor universality, realizes effective response to sudden low-temperature stress of crops in a large area of fields and provides a new technical path for plant cold stress prevention and control.