34 results about "Membrane lipid peroxidation" patented technology

The invention relates to a method for promoting plant seed germination under cadmium stress. The method comprises the treatment of plant seeds through melatonin before germination. The method comprises the following step: enabling the plant seeds to contact with 10 to 250 micron mol/L by concentration of melatonin. With the adoption of the method, the germination potential of the plant seeds, in particular cucumber seeds and switchgrass seeds under the cadmium stress, can be effectively increased; the germination rate can be increased; the seeds can rapidly germinate uniformly; the important topic on the way of enabling normal germination of the seeds in the soil polluted by cadmium in the agricultural production is solved; the method has an important application value in production; melatonin is utilized to pre-treat, so that the expression of antioxidant enzyme activities can be induced, a large amount of free radicals produced under the cadmium stress can be removed, the oxidation stress is relieved, the oxidation damage is reduced, the seeds can be kept in a relatively small membrane lipid peroxidation level, and as a result, the seeds can germinate normally.

The invention relates to a high-temperature drought stress resistance agent and application of the agent and belongs to the field of crop planting. In order to overcome the technical defects that thestress resistance effect is poor by applying glycine betaine alone in the prior art and corresponding substitute products fall short in the market, the provided high-temperature drought stress resistance agent is prepared from glycine betaine, urea and Tween-20. When the agent is applied to annual seedlings or annual or biennial or triennial plants, drop of the chlorophyll content under an adversesituation can all be alleviated, and the photosynthetic capacity of leaves is improved. Accumulation of superoxide radicals (O2 -) in the leaves is reduced, the relative electrolytic leakage and membrane lipid peroxidation level of the leaves are lowered, plant resistance to drought and other stress is improved, and the agent is suitable for application and popularization in the agriculture.

The invention discloses an application of an extremely halophilic archaea (Natrinema altunense sp.) NaSOD gene in improving rice salt tolerance. The base sequence of the extremely halophilic archaea NaSOD is shown by SEQ ID NO.3. The over-expression NaSOD genetically modified rice has the advantages of higher ROS scavenging capability and capability of reducing membrane lipid peroxidation caused by salt treatment so that higher photosynthetic rate can be guaranteed. According to the invention, the extremely halophilic archaea NaSOD gene is transferred into the rice so that the salt tolerance of the rice can be obviously improved.

The invention belongs to the technical field of agricultural production, and particularly relates to a cold-resistant agent for brassica rapa L. The cold-resistant agent for the brassica rapa L. seedlings is mainly characterized by comprising raw materials as follows: 15-25 mg/L of ABA (abscisic acid) and the balance of water, wherein the purity of the ABA (abscisic acid) is higher than or equal to 99.9%. The cold-resistant agent aims to solve the problem that the brassica rapa L. in the northern cold and arid regions is vulnerable to low-temperature freezing damage, growth of the overground part and the root system of the brassica rapa L. is promoted with chemical control means, especially the thickening growth of roots, the outstanding performance refers to increase of dry matter accumulation, and safe wintering of the brassica rapa L. is guaranteed; meanwhile, permeability (relative electrical conductivity) of leaf cell membranes as well as accumulation of membrane lipid peroxidation malonaldehyde can be reduced, so that the low-temperature freezing degree of leaves of the brassica rapa L. is reduced, the cold resistance of the brassica rapa L. is remarkably improved, and the cold-resistant agent has great practical significance on brassica rapa L. production.

The invention relates to a composition containing 5-ALA and mycose and a leaf fertilizer prepared by using the composition. The composition for preparing the leaf fertilizer contains 2000-4000 parts by weight of mycose and 0.5-1 part by weight of 5-ALA. According to the leaf fertilizer containing 5-ALA and mycose, the concentration of the mycose is 2-4g/L, and the concentration of the 5-ALA is 0.5-1mg/L. The 5-ALA and the mycose are compounded at specific concentration, and multiple nutrient elements at specific concentration are used to prepare the compound leaf fertilizer. The 5-ALA and the mycose are synergized and can enhance the content of chlorophyll in the blade in comparison with separately using 5-ALA and the mycose, the photosynthesis of multiple crops is promoted, particularly under adverse situation, the stress resistance of crops can be remarkably enhanced, the growth of plants is greatly promoted, and the yield and quality of crops are improved by maintaining the stability of cells, lowering membrane lipid peroxidation and other physiological ways.

The invention discloses a seed processing device and method for improving the drought resistance of wheat buds. The device comprises an adjustable frequency alternating current high voltage power supply, a reactor and an electrical parameter detection system. The peak voltage of the alternating current high voltage power supply can be adjusted in 0-50 kV, and the frequency is adjustable in 50-1000 Hz; a stainless steel sheet is adopted as a high voltage electrode; a quartz glass sheet used as a discharge medium clings to the bottom surface of the high voltage electrode; and a metal mesh is adopted as a grounding electrode. The high voltage terminal of the alternating current high voltage power supply is connected with the high voltage electrode of the reactor, and the low voltage terminal of the alternating current high voltage power supply is connected with the grounding electrode of the reactor. A digital oscilloscope, a voltage probe and a current probe form the electrical parameter detection system which is used for detecting a discharge voltage and a current signal. The device and the method can promote the germination of wheat seeds under a drought condition and promote the growth of wheat seedlings under the drought condition, the membrane lipid peroxidation level of buds is reduced after discharge treatment, damages of oxygen radicals to the buds are reduced, and the discharge treatment improves the drought resistance of the buds.

The invention discloses an application of an OsDUF6 gene to improvement of drought resistance of rice, and belongs to the technical field of genetic engineering. Through cloning, an unknown functionalstructural domain gene OsDUF6 of rice is obtained, and through heredity transformation, an overexpression plant of the gene is obtained. The overexpression plant of the OsDUF6 is subjected to droughttreatment, and the inventor finds that compared with a wild type plant, the overexpression plant has the advantages that the survival rate is increased, the activity of antioxidase is improved, the membrane lipid peroxidation degree is reduced, the ABA content change is increased, and the content of active oxygen (ROS) is reduced, so that results indicate that overexpression of the OsDUF6 can notably improve the drought resistance of the rice.

The invention provides a spinach preservation method, including the following steps: flushing spinach with distilled water or filtered tap water, removing particulate impurities, and draining at normal temperature until zero water drops; soaking in purple grape peel pigment solution having a concentration of 50-200 mg/L for 1-3 h, taking out, draining until zero water droops, covering with plastic wrap, and storing at 4 DEG C. After the preservation technique is utilized to treat spinach, detection shows that there is no rise in the content of nitrites during five days of storage and the content of nitrites on the fifth day is less than 5 mg/kg; water loss rate and Vc content of the spinach decrease slowly, the activity of antioxidant systematic enzymes SOD, POD and CAT increases, and the content of membrane lipid peroxidation product MDA (methane dicarboxylic aldehyde) decreases; the method enables effectively improved quality of spinach during storage and inhibited production of nitrites, and a new way of preservation and health consumption for spinach is provided.

The invention discloses a method for improving lawn forming quality through applying air-dried sludge substrate. The method comprises the steps of selecting full tall fescue seeds as a lawn planting material, applying 300g, 600g and 900g of air-dried sludge to test fields respectively, and watering the substrate fully to guarantee smooth germination of the seeds at an initial cultivation stage and growth of seedlings at an initial stage; and selecting five plants from treated quadrats after cultivation for 30 days and measuring vertical heights of the seedlings. Experimental results prove that due to application of the air-dried sludge under the natural condition, accumulation of above-ground biomass of the tall fescue is promoted, increase of the lawn plant density is promoted, and the chlorophyll content is increased, so that the lawn quality is improved, meanwhile, the plant membrane lipid peroxidation product content is reduced, and environmental adaptability of lawn plants is improved effectively.

The invention discloses a chemical regulation method for increasing the quality and resistance of sandalwood seedlings and belongs to the field of plant growth regulation. The chemical regulation method includes: selecting and preparing a growth regulation agent, isolating host plants, applying the growth regulation agent, performing seedling management and the like. The chemical regulation methodhas the advantages that the ground diameter and canopy growth of the sandalwood seedlings can be promoted evidently, biomass accumulation is increased, seedling quality indexes (QI) are increased, the synthesizing of leaf photosynthetic pigment is promoted, the content of soluble sugar and soluble protein in leaves is increased, the net photosynthetic rate of the leaves is enhanced, the activityof antioxidant enzymes (catalase, peroxidase and superoxide dismutase) is increased evidently, membrane lipid peroxidation is reduced, stress resistance is enhanced, and economic benefits are increased to the maximum degree; the method is simple to operate, quick in action, practicable and the like.

The invention provides a fresh keeping agent for blueberries as well as a preparation method and application of the fresh keeping agent. The fresh keeping agent consists of 0.03%-0.5% of a galla chinensis extract, 0.5%-1.0% of ascorbic acid and 1.0%-2.0% of chitosan, and the pH of the fresh keeping agent is 2.0-6.0. The fresh keeping agent provided by the invention can significantly restrain botryotinia fuckeliana of the blueberries, effectively relieve membrane lipid peroxidation of picked blueberries, maintain integrity of cell membranes of the blueberries, and enable the storage time of theblueberries to be prolonged to 50 days or above from 20 days through conventional refrigeration, the rotting rate of the blueberries is reduced to 10% or below, the rate of weight loss is controlledto be 5% or below, and relative long-time high-quality fresh-kept storage of the blueberries can be realized. The fresh keeping agent for blueberries is safe to use, the treated blueberries do not have any poisonous and harmful substances, and the fresh keeping agent has the characteristics of being efficient, safe and environmentally-friendly.

The invention discloses novel tea polyphenol induction salt-tolerant application, seed soaking solution and a salt-tolerant method for wheat seedlings. The wheat seedlings are irrigated with the seed soaking solution in salination environments when the wheat seedlings grow and the heights of the wheat seedlings reach 10 cm. The salt-tolerant method includes 1), disinfecting wheat seeds; 2), repeatedly thoroughly washing the disinfected wheat seeds in distilled water; 3), accelerating germination of the wheat seeds; 4), irrigating the wheat seedlings with the seed soaking solution for continuous three days when the wheat seedlings grow and the heights of the wheat seedlings reach 10 cm. The seed soaking solution contains tea polyphenol, and the content of the tea polyphenol is 10-40 micrograms/mL. The novel tea polyphenol induction salt-tolerant application, the seed soaking solution and the salt-tolerant method have the advantages that degradation of chlorophyll in leaves of the wheat seedlings can be obviously deferred under salt-stress conditions, the activity of CAT (catalase) and POD (peroxidase) can be improved, the proline content can be increased, accumulation of membrane lipid peroxidation products MDA can be reduced, and the salt-stress resistance of the wheat seedlings can be ultimately improved.

The invention discloses a mushroom-type food fresh-keeping packaging material and a preparation method thereof. The mushroom-type food fresh-keeping packaging material comprises, by mass, 30-50 parts of polyvinyl chloride, 15-30 parts of polyethylene, 5-10 parts of epoxy soybean oil, 1-6 parts of chlorinated paraffin, 2-6 parts of epoxy linseed oil, 5-10 parts of nanometer silica, 0.1-0.8 parts of sodium bisulfate, 0.5-2.5 parts of dicapryl phthalate, 6-12 parts of pentaerythritol, 2-6 parts of a coupling agent and 1-5 parts of a stabilizing agent. The packaging material improves mushroom immunity, induces a protection enzyme system to start, removes redundant free radicals, inhibits membrane lipid peroxidation, protects membrane structure integrity and delays mushroom aging. The packaging material can inhibit generation of spoilage microorganisms such as bacteria and mould, guarantee mushroom quality and realize fresh-keeping effects.

The invention relates to the technical field of okra planting, and discloses a seedling raising method for okra seeds. The seeds are treated and soaked with saline water having a certain concentration, wherein the soaking concentration and the soaking time determine the salt tolerance of the seeds; if the salt tolerance is too low, seedlings are greatly influenced by the saline and alkaline environment of soil in the growth process, thereby being adverse to the growth; if the salt tolerance is too high, the growth and development of the seedlings are inhibited, the cell membrane permeability is increased, and the membrane lipid peroxidation is enhanced, thereby resulting in ion imbalance and metabolic disturbance, and eventually resulting in decline of production; a used mixed growth regulator obviously improves the germination rate and vital index of the okra seeds; a seedling raising substrate containing 6 soil components includes various minerals and nutrient elements in which the plants are deficient; all the indicators are at the optimal levels; through careful management after emergence of the seedlings, the cultured seeds and seedlings can grow quickly; and the fruit yield and quality of the plants are obviously improved.

The invention belongs to the technical field of fresh-keeping, and particularly relates to a fresh-keeping method for picked fruits and vegetables. According to the method, the picked fruits and vegetables are sequentially subjected to cold shock treatment and packaging treatment, and the gas environment in the package can be changed, so that the consumption of nutrient substances caused by respiration is reduced, the loss of water is reduced, the hardness of the picked fruits and vegetables is maintained, the increase of the weight loss rate is slowed down, and the oxygenolysis of chlorophyll is delayed, and the original color of the picked fruits and vegetables is maintained to a great extent; the fruit and vegetable fresh-keeping agent is capable of delaying yellowing of fruits and vegetables, inhibiting physiological metabolic activities of the picked fruits and vegetables, delaying consumption of soluble solids of the fruits and vegetables, inhibiting membrane lipid peroxidation, reducing accumulation of malonaldehyde and inhibiting concentration change of volatile organic compounds, has a synergistic effect on physicochemical, nutrition and flavor quality of the picked fruits and vegetables, and improves the fresh-keeping effect of the picked fruits and vegetables.

The invention provides a method for relieving the damage of atmospheric ozone to grapes by utilizing sodium bisulfite. The method comprises the following step: spraying a sodium bisulfite solution with the concentration being 0.001-0.1wt% once every other 5-7d in grape planting and growing processes. Due to the adoption of the sodium bisulfite solution with a certain concentration in the method for relieving the damage of atmospheric ozone to grapes by utilizing sodium bisulfite, provided by the invention, influences of ozone stress to the membrane lipid peroxidation degree of grape leaves areremarkably relieved, in addition, sodium bisulfite is low in price, wide in source and commercially-available, the method for relieving the damage of atmospheric ozone to grapes by utilizing sodium bisulfite is initiated, and researches on effects of the method for relieving the damage of the ozone are not proposed in the prior art, and therefore, the method provided by the invention is relatively pioneered, convenient to operate and simple in operation step and can be implemented without any specialized persons so as to be worthy of wide popularization and application.

The invention belongs to the technical field of fertilizer production, and particularly relates to a fertilizer for preventing and treating low-temperature disasters and a preparation method thereof.The fertilizer comprises the following raw materials in parts by weight: 16-20 parts of potassium feldspar, 6-10 parts of phosphogypsum, 33-37 parts of a chestnut flower extraction matter, 7-9 parts of an earthworm extraction solution, 21-23 parts of an isatis root extraction matter, 21-25 parts of a grape skin fermentation matter, 41-45 parts of a rice bran ash enzymolysis matter, and 400-500 parts of water. By carrying out extraction and decomposition on natural substances, the purpose of adjusting osmotic balance of crops can be achieved, at the same time, beneficial microorganisms are attracted to gather in the root system, vitality of the root system can be improved, absorption is promoted, accumulation of superoxide radicals in crop cells is beneficial to be cleared, damage of membrane lipid peroxidation is reduced, and at the same time, antioxidants are used, activity of the enzyme system is protected, and breeding of pathogenic bacteria is inhibited, so that the ability of thecrops to resist low temperature stress is effectively enhanced.