The invention discloses a chemical regulation method for increasing the quality and resistance of sandalwood seedlings and belongs to the field of plant growth regulation. The chemical regulation method includes: selecting and preparing a growth regulation agent, isolating host plants, applying the growth regulation agent, performing seedling management and the like. The chemical regulation methodhas the advantages that the ground diameter and canopy growth of the sandalwood seedlings can be promoted evidently, biomass accumulation is increased, seedling quality indexes (QI) are increased, the synthesizing of leaf photosynthetic pigment is promoted, the content of soluble sugar and soluble protein in leaves is increased, the net photosynthetic rate of the leaves is enhanced, the activityof antioxidant enzymes (catalase, peroxidase and superoxide dismutase) is increased evidently, membrane lipid peroxidation is reduced, stress resistance is enhanced, and economic benefits are increased to the maximum degree; the method is simple to operate, quick in action, practicable and the like.