192 results about "Microcystis" patented technology

The invention relates to a preparation and the preparation method for restoring the microecological bacteria on the interface layer of water-sediment. The microecological bacteria preparation uses 0.2-0.4mm of medical stone as carrier, absorbs and fixes the composite microbe optimally combined by bacillus subtilis, bacillus licheniformis, bacillus megatherium, and thin yellow streptomyces. The amount ratio of bacillus subtilis to bacillus licheniformis to bacillus megatherium approximates to one to one to one; the volume ratio of the mixture liquid of the three kinds of bacillus to the thin yellow streptomyces liquid is two to one. The invention has the advantages that spreading the preparation of the invention into the interface layer of functional water body water-sediment in the spring March to April each year can prevent blue algae (aerugo micro-capsule algae is the most) from fulminantly growing in summer June to August. The preparation can improve the quality of water, can prevent eutrophication development, and can promote the carbon, nitrogen, and oxygen circulation on the interface layer of water-sediment. The microecological bacteria preparation has high effective colony amount and strong adaptability; and is effective to restore the microecological environment at the interface layer of water-sediment.

The present invention relates to an airlift type photobioreactor which solves problems of complicated structure, poor gas-liquid mixing efficiency and low microcystis fixing CO2 speed rate in the existing reactor. The reactor includes a glass tank equipped with a culture medium intake, a nutrient fluid intake, a cooling water outlet, an air intake and an air outlet, the glass tank inner side is annual equipped with multi-group recuperator tubes communicated with the cooling water intake and the cooling water outlet, an ascending conduit tube divides the glass tank into an ascending area and a descending area, the glass tank top is equipped with an air separating area, the air inlet communicates with an air distributor located on the ascending area bottom through the air inlet tube, the descending area is equipped with a nutrient fluid outer circulation pipeline, the nutrient fluid outer circulation pipeline communicates with the air inlet pipeline through a water pump. The photobioreactor has simple structure and convenient operation which is particular suitable for photosynthesis fixing CO2 with high efficiency by using microcystis in CO2 emission reduction system. The emission reduction speed rate can reach above 50%, and has low operation cost and is friendly to environment.

The present invention belongs to the technical field of environment protection, in particular to a method to degrade Microcystic aeruginosa through microorganisms. The method is that firstly an algae-lysing bacterial strain Brevibacillus spp.FDK2 can be achieved by separating from and sublimating deep eutrophication urban lake water body;the algae-lysing bacterial strain Brevibacillus spp.FDK2 is cultured by a culture medium to acquire bacterial liquid with a certain concentration. The bacterial liquid is added into a water sample containing Microcystic aeruginosa. Under a certain condition, the bacterial strain has a good degrading effect on the Microcystic aeruginosa. The degrading removing rate is more than 90 percent.

The method includes following steps: to filter and clear alge in water by sand to reduce quantity of suspended substances and microcap frustule in water; next is plant absorbing process, a section of organic substances and microcystim are absorbed and decomposed by sandy soil plate which hygrophytes are planted on; the third is to further get rid of suspended substances and microcap frustule in water by active carbon adsorbing installation; the fourth is ozone process by blowing in high-density ozone containing air; the fifth is to detect content of microcystim in drinking water processed by method of high-effective liquid phase chromtograph detection.

The invention discloses a construction method and an application of an immobilized biological bacterium agent for a micro-polluted water source and belongs to the field of ecological remediation of water environments. The method is a treating process for purifying a water body through construction of the biological bacterium agent, and ammonia nitrogen, iron, manganese, microcystis, algal toxins and the like in the water body can be removed effectively. The method comprises steps as follows: (1) rescreening and compounding of multifunctional oligotrophic ammonia nitrogen bacteria; (2) screening of efficient algicidal bacteria; (3) fermentation and construction of the bacterium agent; (4) bacterium agent immobilization and biological enhancement; (5) research on dosing methods of the bacterium agent, and treatment and biological enhancement of the micro-polluted drinking water source. The biological bacterium agent is constructed with an immobilization technology, after a system runs stably, the removal rate of the ammonia nitrogen is higher than 80%, removal rates of the microcystis and the algal toxins are higher than 40%, the removal rate of the iron in water is 50%, and the removal rate of manganese is 51%. The method can be combined with actual projects and is wider in application prospect.

The present invention discloses a method for culturing golden algea and an application thereof in controlling blooming algae, which belong to the field of water pollution control technology. The method for culturing golden algea is as follows: golden algea and microcystic aeruginosa are added into the BG11 culture medium; the golden algea lives on the microcystic aeruginosa to grow heterotrophically; after seven to ten days of culturing, the microcystic aeruginosa is gradually eaten up; the golden algea gets into the stable growing period; high-density golden algea liquid is obtained; and the golden algea liquid is centrifugated in order to harvest the golden algea. The golden algea harvested by centrifugating or the golden algea liquid is directly added into the water body, and the golden algea swallows the blooming algae, so that the overgrowth of the blooming algae can be controlled and the blooming algae can be eliminated. The method which utilizes the golden algea to swallow the blooming algae to prevent the outbreak of the blooming algae and control the growth of the blooming algae is an algae-inhibiting method which is characterized by good ecological safety, cheap materials, economy, high efficiency and easy utilization, and has the advantages of easy golden algea culturing, good algae control effect, etc.

The invention discloses an algae inhibitor for verdigris microcystis aeruginosa. The algae inhibitor is composed of n-nonanoic acid and linoleic acid in the volume ratio of 1:1, the applicable concentration range of the algae inhibitor in verdigris microcystis aeruginosa ranges from 0.04mL/L to 0.08mL/L. Compared with the prior art, two different kinds of fatty acids, namely the n-nonanoic acid and the linoleic acid, are selected to be combined, after inhibition effects of the combined fatty acids are evaluated by means of three kinds of different evaluation systems, the result indicates that the combination of the fatty acids has excellent synergic inhibition effects indeed. Further, the algae inhibitor is low in cost, environment-friendly and safe.

The invention discloses a seawater spirulina selenium-rich culture method. The method comprises the following steps of: selecting one or combination of more of the seawater-naturalized spirulina platensis, spirulina maxima and microcystis aeruginos as the algae seed; based on the natural seawater, artificial seawater or the mixture of the two at any ratio, adding a selenium element with concentration of 1-160mg/L to serve as a culture medium; inoculating the algae seed into a photobioreactor containing the culture medium for culture; and then collecting the cultured spirulina body to obtain the seawater spirulina which is the selenium-rich seawater spirulina. Through the invention, the method is simple, the selenium addition concentration is low, the culture medium can be recycled for a long time, the operation is simple and convenient, the selenium enrichment ability is strong, the cost is low, the method is green and safe, etc. The bioactive substance of the seawater spirulina generates a synergistic effect with organic selenium in the algae body to realize the function, and can be applied to various diseases caused by selenium lack and oxidative damage of oxygen free radical for the selenium supplement additive, oxidation resistance, aging resistance, tumor resistance, Keshan disease prevention and treatment and the like.

The invention relates to a method for inhibiting cyanobacterial bloom, belongs to the fields of aquaculture industry and environmental science, and particularly provides a method for inhibiting cyanophyta microcystis bloom, which is characterized by comprising the following steps: (1) monitoring the nitrogen element concentration of water in which cyanophyta microcystis bloom occurs; (2) aerating the water continuously to maintain the oxyty of the water; (3) adding carbonaceous saccharides during aeration, wherein the addition concentration depends on the total nitrogen concentration of the water; (4) adding silicon compounds into the water during addition of the saccharides; (5) ensuring that cyanobacterial bloom particles become smaller and smaller, and the water becomes more transparent after 2 to 3 days; (6) adding saccharides again in the 7th to 10th days to ensure that the cyanobacterial bloom particles become smaller and less. Through the adoption of the method, cyanobacterial bloom in the water is effectively inhibited; the transparency of the water is improved; the significances in environment protection and ecologic protection are both high.

The invention relates to an inhibition method of microcystis aeruginosa. A cordate houttuynia extract is used for inhibition. The inhibitor raw material of the inhibition method is rich and easy to get and environment-friendly during the treatment process of cyanobacterial bloom, and does not cause secondary pollution on water.

The invention provides a strain of stenotrophomonas sp., which is named as stenotrophomonas F6, and has a preservation number of CGMCC No.6547. Two active algicidal substances, namely cyclo(glycine-proline) and hydroquinone, are separated from the metabolism products of the stenotrophomonas F6, and then the substances are purified and identified. The half effective concentration of the cyclo(glycine-proline) on inhibiting microcystis aeruginosa 9110 is 9.5 mg/L, and the cyclo(glycine-proline) has no inhibiting effect on synechococcus BN60. The half effective concentrations of hydroquinone on inhibiting microcystis aeruginosa 9110 and synechococcus BN60 are 0.96 mg/L and 5.6 mg/L. The stenotrophomonas F6 and the active algicidal substances (cyclo(glycine-proline) and hydroquinone) can be applied to the development and production of novel algicide, and finally are applied to the control of cyanobacterial bloom in fresh water.

The invention discloses a method for discriminating an overwintering-recovery period boundary of overwintering cyanobacteria. The method comprises the following steps of carrying out RNA extraction and purification on a water sample from a cyanobacteria bloom region and indoor cultured microcystis; carrying out reverse transcription to obtain cDNA; with the cDNA as a template, carrying out real-time fluorescence quantitative PCR amplification; amplifying genes of microcystis gas vesicles by virtue of a primer gvpC; specifically amplifying microcystis 16srRNA reference genes by virtue of a primer Micr; after the PCR is completed, exporting correspondingly threshold cycle values of which the difference values are taken as corrected cell copy numbers, wherein the difference between the cell copy number of the wild sample and the cell copy number of the indoor sample is taken as a relative cycle number of the wild target genes and marked as deltadelta Ct; calculating the relative expression level by virtue of 2-deltadelta Ct, plotting a curve with time as an abscissa and the values of 2-deltadelta Ct as an ordinate and determining the overwintering-recovery period boundary of overwintering cyanobacteria according to the curve. By the method, a plurality of samples can be simultaneously analyzed and the method has the advantages of good repeatability and accuracy and short detection time.

The invention is a flocculant for removing Cyanobacteria bloom microcystis, a preparation method and applications thereof, wherein the flocculant is prepared from clay and chitosan. The preparation method comprises: drying clay, crushing to achieve a fineness of 100 [mu]m, dissolving chitosan by using hydrochloric acid having a concentration of 1% (wt%) to obtain a 1 mg/mL chitosan hydrochloric acid solution, sufficiently impregnating with the clay powder, and drying to obtain the flocculant. The invention provides the modified clay flocculant for removing the microcystis during the generation of Cyanobacteria blooms, the preparation method and applications thereof, wherein the microcystis in the Cyanobacteria bloom water can be rapidly and rapidly removed, the emergency treatment on the Cyanobacteria bloom generation can be achieved, the removing effect is good, the effect is rapid, the operation is simple, and the important theoretical and practical significance is provided for the rapid treatment of the Cyanobacteria bloom generation; and the clay around the Cyanobacteria bloom generation soil can be used to modify, such that the long-distance transportation cost of the flocculant can be saved, and the economic benefits are impressive.

The invention belongs to the field of water purification and relates to a bacillus pumilus with efficient alga-lysing activity and application thereof. The bacillus pumilus NMCC46 with efficient alga-lysing activity is preserved in the China General Microbiological Culture Collection Center with the preservation No. as CGMCC No 3378 and the preservation date as Nov. 2, 2009. The bacillus pumilus NMCC46 has a very strong restraining effect to the growth and reproduction of microcystis aeruginosa, and has the algal inhibiting rate of 97.45% after 7 days. The fermentation supernate of the bacillus pumilus NMCC46 has an excellent alga-lysing effect at the final concentration of 0.5%. Alga-lysing active ingredients generated by the bacillus pumilus have excellent heat resistance and pH stability. Therefore, the bacillus pumilus NMCC46 can be used for controlling cyanobacterial bloom.

The invention relates to a polyculture type microalgae cultivation method capable of inhibiting growth of microcystis aeruginosa. Secondary effluent water of an urban sewage plant serves as a culture medium, the nontoxic microalgae and toxic cyanobacteria are subjected to joint culture in the secondary effluent water of the urban sewage plant, the density proportion between the initial nontoxic microalgae and toxic cyanobacteria is controlled to be (2:1)-(10:1), in the culture process, sodium nitrate and monopotassium phosphate serve as complementation sources of nitrogen and phosphorus respectively, the concentration of the culture medium is controlled to meet the conditions that total nitrogen (TN)=14.0-16.0 mg.L<-1>, total phosphorus (TP)=0.28-0.40 mg.L<-1>, and the proportion between nitrogen and phosphorus is (40-50): 1. Compared with the prior art, the polyculture type microalgae cultivation method is applicable to nontoxic microalgae culture, inhibition to growth of the microcystis aeruginosa is achieved, and the method is a safe, reliable and economic cultivation method; besides, according to the technical method, the secondary effluent water of the urban sewage plant serves as a culture medium source, and the function of deeply purifying sewage is achieved.

The invention discloses an aga lysing biological preparation and an application thereof. The preparation is prepared through mixing Bacillus subtilis, Bacillus licheniformis, Bacillus laterosporus, Streptococcus faecalis, photosynthetic bacteria, Sphingomonas sp., vitamin C, vitamin E and lactose. The above product has good stability and high efficiency, and consumes an extremely low amount of oxygen and rapidly degrades microcystis and other pollutants in culture water in the application process in order to effectively improve the environment of the water. The preparation is nontoxic and harmless to environment, people and animals, has no residuals, can substantially improve and purify the environment and the culture body and effectively avoid bursting of cyanobacterial bloom, and also can improve the immunity of aquatic product animals and reduce the incidence of diseases to make the ecologic system of the whole culture water tend to balance and go well.

The invention discloses a method for extracting a microcystis aeruginosa extracellular polymer, The method comprises the following steps of taking an algae liquid of the microcystis aeruginosa, and performing centrifuging to obtain a dissolved type extracellular polymer; suspending the lower layer residue in a buffer solution, and performing centrifuging to obtain a loose attached type extracellular polymer; suspending the lower layer residue in an NaCl buffer solution, and adding sodium hydroxide, adjusting the pH value to be 11, and under the conditions of 4 DEG C and 80-120 rpm, performingstirring for 10-20 minutes, and then putting the mixture into a water bath at 40-60 DEG C for heating for 20-40 minutes; and performing centrifuging on the buffer solution to obtain a tightly combinedtype extracellular polymer. According to the method, the extracellular polymer is extracted by adopting a combination mode of "NaOH and heating" on microcystis aeruginosa, and the parameter extraction conditions are strictly controlled, so that a large amount of EPS in the algae be extracted, the algae cells can not be damaged, and pollution of the EPS is not caused, and therefore, the effectiveEPS component is extracted.

The invention discloses a rapid microcystin extracting and detecting method comprising the following steps: firstly, conducting pretreatment on algae samples, removing impurities on microcystis flosaquae samples, cleaning the microcystis flosaquae repeatedly by distilled water, and filtering the microcystis flosaquae; secondly, conducting homogenate process and heavy weight measurement, adding algae slurry concentrated in the step one into the distilled water, stirring the slurry uniformly by a homogenizer, taking the algae slurry additionally for measuring the dry weight; thirdly, extracting toxin by methanol one-step method, calculating the measuring result of dry weight of samples in the step two, taking the algae slurry with known dry weight and volume, stirring, vibrating and mixing the algae slurry, then standing and extracting the algae slurry in a refrigerator; fourthly, conducting centrifugal purification, conducting centrifuge on extracted liquid processed in the step three, obtaining supernate, and obtaining clarified extraction liquid; fifthly, using a filter for filtering the supernate obtained from the step four; sixthly, conducting HPLC (High-performance liquid chromatography) detection, adopting optimized chromatograph condition for analyzing the toxin rapidly. The method has the advantages of high reliability, simple needed instrument, convenience in operation, high detection efficiency and the like, and is suitable for large-batch toxin extraction and analysis in a laboratory.

The invention provides an immobilized algae-lysing microorganism as well as a preparation method and an application thereof. The immobilized algae-lysing microorganism is prepared from raw materials in percentage by mass as follows: 40%-60% of algae-lysing microorganisms, 30%-50% of a carrier and 10%-20% of an immobilization material, wherein the algae-lysing microorganisms are one or more of bacillus, streptomyces, pseudomonas, sphingomonas, mycobacterium and shewanella with algae-lysing activity and are liquid, powdery or granular substances, with the viable count not lower than 1.0*10<10> cfu/g. The immobilized algae-lysing microorganism has a large specific surface area, good adsorption performance, high algae-lysing efficiency and environment adaptability, no secondary risk and the like, particularly has remarkable algae-lysing effects on microcystis, anabaena, aphanizomenon and other typical harmful algae and has better application prospects in the field of bioremediation of eutrophic water.

The invention provides a recombinant mortierella alpina strain for heterologous expression of an MpFADS6 gene by virtue of a delta-6 desaturase gene MpFADS6 from tiny microcystis. The invention also provides an application of the recombinant mortierella alpina strain in production of EPA. By adding 1% of peony seed oil or 50g/L of peony seed pulp juice into a culture medium, the yield of the EPA is effectively increased, which has important significance for basic theory research of metabolic regulation in synthesis of fatty acid of an oil-producing fungus namely mortierella alpine and production of PUFAs. Compared with over-expression of an omega 3 fatty acid desaturase gene in the mortierella alpine, the yield of the EPA is increased by 5 times and can reach 588.5+/-29.6mg/L.

The invention discloses an algistat, which is an absolute methanol crude extract of onion stalks, an absolute acetone crude extract of the onion stalks, an absolute petroleum ether crude extract of the onion stalks or an ethanol crude extract of the onion stalks. Compared with the prior art, the crude extracts from the onion stalks by the organic solvents perform an inhibitory effect to microcystis aeruginosa from high to low as follows: the inhibitory effect of absolute acetone is larger than that of 75% ethanol, the inhibitory effect of the 75% ethanol is larger than that of absolute petroleum ether, and the inhibitory effect of the absolute petroleum ether is larger than that of absolute methanol. The inhibition ratio of the absolute acetone pure extract of the onion stalks is enhanced along with the time, and a concentration effect is shown. The inhibition ratio of a high dose group on the fourth day can be up to 97.7%. EC50 (50% effective concentration) computed on the fifth day is 0.03g/L, which proves that the algal inhibition effect of the purified absolute acetone extract is more obvious than that before purification.

The invention belongs to the field of environmental microbial restoration, and discloses a method for synchronously dissolving algae/degrading algal toxins by using a microbial combined preparation. According to the method, a Pseudomonas aeruginosa fermentation liquid is utilized to dissolve microcystis aeruginosa in the logarithmic growth phase, and a Pycnoporus sanguineus spore liquid is utilized to degrade residual microcapsule algal toxins in the alga dissolution system, thereby achieving the goals of synchronously removing algae and degrading metabolites. The method is simple and is low in cost. The fermentation time is 48 hours, and the alga dissolution time is 7 days. When the degradation time is 7 days, the alga dissolution effect reaches 83.31%, and the degradation efficiency of microcapsule algal toxins reaches 67.63%, thereby achieving the goal of synchronously removing algae and degrading metabolites. The method has high practical application value, and provides references for solving the problems of lake/reservoir water eutrophication and drinking water deep treatment.

The invention discloses a bacillus subtilis strain capable of killing algae and application thereof. The strain is named (Bacillus subtilis) BLS1 and was collected in China Center for Type Culture Collection in Wuhan University on December 18, 2012, with a CCTCC No. of M2012534. The strain is capable of effectively killing harmful algae and has an obvious killing effect on microcystis aeruginosa which is most harmful to the body of fresh water so that algal bloom and red tide can be relieved and avoided in time. The application method of the strain disclosed by the invention is simple; the strain can be made into powder or tables according to a conventional method and can be put into the body of water anytime as required; in case of no red tide or algal bloom, the strain can be put into the body of water to balance floras in the body of water; the strain is safe to animals bred in the body of water, is mild in fermentation condition and can be put into culture enlargement easily.