106 results about "Brown algae" patented technology

The invention belongs to the field of agricultural chemistry and bio-fertilizer, and discloses a fertilizer synergist based on polyaspartic acid, alginate oligosaccharide and mannan oligosaccharide and a preparation method of the fertilizer synergist. According to the synergist, polyaspartic acid derived from plant source amino acid, alginate oligosaccharide extracted from natural brown algae and mannan oligosaccharide extracted from plants are used as main raw materials, and the polyaspartic acid, the alginate oligosaccharide and the mannan oligosaccharide cooperate to form an induction-complexation-adsorption three-effect-in-one mechanism, wherein the alginate oligosaccharide and the mannan oligosaccharide induce plant root activity and stress resistance gene expression; the polyaspartic acid is dominated to form a multi-point weak complex network, so that slow release and controlled release of nitrogen and potassium ions are realized; the three components cooperate on the surface of soil colloid to form an adsorption layer, and nutrient leaching loss is reduced. The preparation process adopts a green process of'enzymatic pretreatment-segmented complexing-low-temperature forming ', so that the oligosaccharide activity is maintained, and the use of an organic solvent is avoided. The obtained synergist can significantly improve the nitrogen utilization rate and the root activity and prolong the fertilizer efficiency period, and is suitable for green and efficient agricultural production of various crops.

The invention discloses a method for determining the content of monosaccharide in seaweed, and belongs to the technical field of biological detection. According to the method, an ion chromatography sugar separation system is adopted, a pulse ampere detector is combined, CarboPac PA20 is used as a sugar analysis column, and the eight monosaccharide components in the seaweed can be detected under the limited extraction and purification method. The method is low in detection limit, good in stability and suitable for measuring the content of free monosaccharide and polysaccharide hydrolyzed monosaccharide in common algae (such as brown algae, green algae and red algae).

The invention provides a temperature-increasing oxygen-increasing agent for waterlogging type medium and low yield fields and a preparation method of the temperature-increasing oxygen-increasing agent. The temperature-increasing oxygen-increasing agent is prepared from the following raw materials in parts by weight: 80 to 130 parts of tea seed cakes, 80 to 110 parts of aged chicken manure, 140 to 180 parts of straw powder, 7.7 to 10 parts of beneficial bacteria, 0.3 to 0.8 part of alginate oligosaccharide, 9.5 to 12 parts of silicon-calcium-magnesium ore powder, 200 to 220 parts of activated humic acid and 340 to 430 parts of calcium peroxide. The fertilizer has comprehensive nutrients, can effectively improve the utilization rate of the fertilizer, improve the soil structure and promote production and income increase, takes organic matters as a carrier, is added with a large amount of activated humic acid and silico-calcium-magnesium ore powder, has strong chelating ability, promotes the formation of soil aggregates and improves the absorption and utilization rate of plant nutrients, and has good application prospects through a unique coating technology. The active ingredients and the granulation rate of the product are effectively improved.

A soil conditioner belongs to the technical field of soil improvement, and comprises, by mass, 8-12 parts of chitosan oligosaccharide, 2-3 parts of cellulose oligosaccharide, 5-10 parts of alginate oligosaccharide, 2-6 parts of licorice oligosaccharide, 30-40 parts of humic acid, 5-8 parts of a microbial agent, 10-20 parts of minerals, and 5-10 parts of manganese oxide modified charcoal. According to the invention, oligosaccharide is used as an inducer, humic acid is used for constructing a soil skeleton, microorganisms are used for realizing nutrient circulation, and mineral substances and manganese oxide modified charcoal are used for providing long-acting support, so that biostimulation is used as a guide, the soil structure is adjusted, microbial flora is improved, and the crop yield is increased; the heavy metal polluted soil condition is improved.

The invention discloses a bio-organic fertilizer for enhancing disease resistance of plants, and relates to the field of bio-organic fertilizers. The organic fertilizer is prepared by compounding brown algae residues, corn straws, composted chicken manure, sodium lignin sulfonate, a compound microbial agent and alginic acid-carboxymethyl cellulose composite gel. The preparation process comprises the following steps: a raw material pretreatment stage: crushing and drying brown algae residues, mixing the crushed and dried brown algae residues with corn straws subjected to alkaline hydrolysis treatment and decomposed chicken manure, and adding sodium lignin sulfonate for pre-fermentation; the compound microbial agent is compounded after being cultured step by step through bacillus subtilis and streptomycete; alginic acid and carboxymethyl cellulose are subjected to ionic crosslinking and ultrasonic treatment to form a three-dimensional gel network; mixing the pretreated substrate, the complex microbial inoculant and the gel, performing three-stage fermentation of heating, high temperature and cooling, granulating, and forming the slow-release particles by adopting a starch-alginic acid coating.

The invention belongs to the technical field of preparation of feed additives, and particularly relates to a method for preparing seaweed-based polysaccharide for feed based on composite low-value brown algae. According to the method, multiple kinds of composite low-value brown algae are adopted as raw materials for the first time, composite alkali liquor is creatively heated to assist ultrasonic extraction to prepare the seaweed-based polysaccharide for the feed, and in some embodiments of the method, compared with an ultrasonic extraction method without alkali heating, the polysaccharide extraction rate is increased by 1.2 times through composite alkali liquor heating to assist ultrasonic extraction. The used raw material is low-value brown algae which is wide in source and low in cost; the preparation process is simple to operate, clear in parameters of each step and easy to realize industrial production. The prepared seaweed-based polysaccharide for the feed is convenient to add in the feed, can remarkably improve the growth performance of animals, enhance the immunity and improve the intestinal health, has good economic benefits and social benefits, has wide application prospects in the feed industry, and is high in practicability.

The invention provides a stepped directional extraction method of fucoidan, and relates to the technical field of extraction of active ingredients of algae. The method comprises the following steps: pretreating brown algae powder by microwaves and ultrasonic waves, mixing the pretreated brown algae powder with a deep eutectic solvent, and carrying out ultrasonic extraction to obtain algin; the eutectic solvent is composed of choline chloride and lactic acid according to the molar ratio of 1: (1-3). According to the method disclosed by the invention, a microwave-ultrasonic synergistic wall-breaking coupling deep-eutectic solvent extraction process is adopted, so that the yield of algin is increased to 73.2%, meanwhile, an additional toxic chemical chelating agent does not need to be added, the effect of effectively adsorbing heavy metal ions is achieved, and the biological safety and commercial value of the product are greatly improved.

The invention relates to the technical field of open tile windows, in particular to an open tile window with sea moon as a raw material and a preparation method thereof, and the open tile window comprises a base material layer, a functional layer and a supporting structure; the base material layer is made of a transparent plate which is formed by mixing and hot-pressing a raw material and modified nano cellulose; the aurelia raw material is prepared by mixing a seaweed extract and pretreated aurelia shell powder; the functional layer is arranged on the surface of the base material layer and is a composite film prepared by a chemical vapor deposition process; the supporting structure is an aluminum alloy frame and is combined with the base material layer through laser welding. According to the invention, the raw material components are optimally proportioned, so that the components play a targeted role in improving the performance of the open tile window, and a foundation is laid for the overall performance. Wherein the sea moon raw material is composed of an algin extract and pretreated sea moon shell powder, and sodium alginate in the algin extract has excellent cohesiveness and light transmission.

The application provides a kind of cement-like solidification characteristics and mineral flavor seaweed mayonnaise and its preparation method, based on the principle of multistage homogenization in cement production, heat treatment and particle grading, porous activated carbon particles prepared by carbonization of brown algae fermentation are graded and matched, combined with ladder type homogenization emulsification process, the cement-like solidification gel characteristics are realized;The activated carbon particles of brown algae are calcined at high temperature to form a multistage pore structure, and form a cement-like mineral flavor in cooperation with black pepper powder, smoked chili powder, clove powder, litsea cubeba powder, catnip powder and garlic powder, etc.The product prepared by the application has unique touch, low-fat characteristics and functional dietary fiber, and is suitable for building theme catering and health food field.

The present application relates to the field of pharmacy, disclose the medicine composition for antibacterial and anti-inflammatory of Chinese herbal medicine and marine extract combination, including: baicalin extract 5~10 parts; andrographolide extract 3~7 parts; sulfated fucoidan 2~5 parts; spongin extract 0.2~1 part; wherein: baicalin extract is derived from the root of scrophulariaceae, is extracted by ethanol-water mixed solvent; andrographolide extract is derived from the whole plant of andrographis, is extracted by ethanol-water mixed solvent; sulfated fucoidan is derived from brown algae, is prepared by enzymolysis and alcohol precipitation method; spongin extract is derived from sponges, is extracted and separated and purified by organic solvent. Through the technical scheme of the synergistic combination of active ingredients of Chinese herbal medicine and marine extract, the technical effect of destroying the biofilm barrier and clearing the stubborn infection focus in the treatment of bacterial vaginitis, candida vaginitis and cervical inflammation associated with biofilm infection is achieved.

The invention discloses a microbial fertilizer for relieving continuous cropping obstacles of celery and application of the microbial fertilizer, and belongs to the field of biological agriculture and resource environment. The microbial fertilizer provided by the invention comprises the following components: bacillus subtilis, bacillus velezensis, paenibacillus polymyxa, trichoderma harzianum, glucose, polyacrylamide and alginate oligosaccharide. Experimental results show that the microbial fertilizer is applied to stem bases 15 days after celery seedling emergence and applied to planting furrows or planting holes before seedling culture celery transplanting, the seedling protection rate and yield of celery in continuous cropping soil are remarkably increased, and the celery planting cost is reduced by strengthening disease prevention and treatment. The microbial fertilizer has the advantages of low cost, safety, no toxicity, environment friendliness and the like, and the fertilizing method is simple, convenient and feasible. The invention provides a new method for relieving celery continuous cropping obstacles, and has remarkable economic benefits and wide application prospects.

The invention discloses a special water and fertilizer integrated organic fertilizer for rosa roxburghii tratt and a fertilization method. The feed additive is prepared from the following components in parts by mass: 3-5 parts of glucose, 3-5 parts of bacillus subtilis, 5-8 parts of aspergillus niger, 5-8 parts of lactobacillus plantarum, 10-16 parts of saccharomycetes, 1-3 parts of cellulase, 12-18 parts of papain, 20-30 parts of roxburgh rose pomace, 45-60 parts of brown algae, 45-60 parts of deep-sea fish leftovers and 80-100 parts of purified water. The preparation method comprises the following steps: crushing and mixing the raw materials, fermenting for 5-8 days, and filtering to obtain the brown water-soluble fertilizer. The special organic fertilizer avoids the problems of poor solubility and pipeline blockage of organic fertilizer products, realizes cyclic utilization of the roxburgh rose pomace, and enhances the environmental adaptability of soil; rosa roxburghii growth is promoted, and yield is increased.

This invention belongs to the field of pharmaceutical technology, specifically relating to an alginate oligosaccharide, its preparation method, and its applications. Using brown algae as raw material, the invention first removes impurities from the algae, then extracts it through subcritical acid water, followed by neutralization, decolorization, heavy metal removal, membrane ultrafiltration / nanofiltration purification, and spray drying to obtain high-purity saturated alginate oligosaccharides with a specific degree of polymerization distribution. These alginate oligosaccharides can increase butyrate production by intestinal flora and exert a laxative effect. The alginate oligosaccharide and its preparation method provided by this invention directly use brown algae as raw material, overcoming the drawbacks of traditional methods that first prepare alginate and then hydrolyze it to prepare alginate oligosaccharides, greatly shortening the process steps and improving production efficiency. The alginate oligosaccharides provided by this invention have high purity and broad application potential in the preparation of intestinal health drugs, health products, or dietary supplements.

The invention discloses preparation and application of algal polysaccharide-based co-drug-loading tumor targeting nanoparticles. The algal polysaccharide-based co-drug-loaded nano particle disclosed by the invention is a self-assembled targeting nano particle co-constructed by biotin modified algal polysaccharide sulfate and arginine modified chitosan. The algal polysaccharide-based co-drug-loaded nano particle disclosed by the invention is an algal polysaccharide-based co-drug-loaded nano particle. The algal polysaccharide-based nanoparticles encapsulate anti-tumor hydrophilic drugs and hydrophobic drugs to realize targeted co-delivery of the drugs in solid tumor tissues, self-assembly is generated through electrostatic interaction of polysaccharide molecules to drive the encapsulated drugs to enter inner cores of the nanoparticles, and meanwhile biotin molecules are mainly distributed in shell layers of the nanoparticles. According to the algal polysaccharide-based co-drug-loading nano particle, the targeting property to solid tumor tissues is enhanced, the defect that a conventional drug-loading system can only encapsulate a single hydrophilic or hydrophobic drug is innovatively overcome, efficient co-delivery of hydrophilic and hydrophobic drugs with different attributes is realized, and proliferation and migration of tumors can be remarkably inhibited.

The application discloses a kind of anti-reverse tolerance of poor root flower planting slow-release fertilizer and its preparation method, the slow-release fertilizer includes 30-45 parts of seaweed residue, humic acid 15-25 parts, diatomite 10-20 parts, oyster powder 5-15 parts, plant protein glue 3-8 parts, polyglutamic acid 1-3 parts, bacillus mucilaginosus powder 0.5-2 parts, brown algal oligosaccharide 0.1-0.5 parts, potassium silicate 2-5 parts, ammonium molybdate 0.05-0.15 parts, silicon phytic acid ester 0.5-2 parts, by introducing silicon phytic acid ester, fertilizer particles can be adjusted according to soil humidity release rate of nutrients, lock up nutrients in drought, accelerate release in wet; Absorption and fixation harmful metal ions in soil, reduce stress, and slowly hydrolyze in slow-release process, continuously provide beneficial substances, synergistically improve the fertilizer utilization rate of root flower in poor soil, resistance tolerance and sustained growth capacity.

This invention belongs to the field of cosmetic technology, specifically relating to a gentle exfoliating and brightening composition and its application. Specifically, this invention uses a combination of protease aqueous solution, probiotic-fermented brown algae, and panthenol to prepare a composition; then, using this composition as the main active ingredient, it is prepared into a skincare cosmetic. Experimental verification has shown that it can effectively restore the skin's microecological balance, while also brightening the skin. Furthermore, compared to conventional acid-based exfoliating ingredients like salicylic acid, it has a stronger exfoliating effect, a more pronounced exfoliating effect, and is gentler, thus possessing significant practical application value.

The invention belongs to the technical field of food preservation, and provides a gelatin / chitosan film loaded with brown algae extract and a preparation method and application thereof, and the preparation method comprises the following specific steps: (1) mixing brown algae with an ethanol solution, and concentrating to obtain the brown algae extract; (2) mixing a gelatin solution, a chitosan solution, glycerol and the brown algae extract to obtain a film precursor; and (3) carrying out tape casting on the film precursor to obtain the gelatin / chitosan film loaded with the brown algae extract. The brown algae extract is rich in effective active ingredients such as organic acid, so that the film is endowed with good antioxidant and antibacterial properties. By adding the extract, the mechanical property, the water vapor barrier property and the thermal stability of the composite film are effectively improved, and an active packaging material with a uniform structure is formed. In fresh-keeping application, the film effectively delays the quality deterioration process of the instant sea cucumbers in the normal-temperature storage period through the active function of the film, and the texture and sensory quality of the product are remarkably maintained.

The invention provides absorbent articles which are formed from or which incorporate a cellulose / alginate-containing material, wherein said material comprises cellulose fibres and a residue of alginate from a brown seaweed, and wherein said material is obtained by a process comprising at least the following steps: (i) providing a brown seaweed, or part thereof; (ii) dividing the brown seaweed, or part thereof, into a plurality of portions; (iii) washing the plurality of portions with water whereby to remove water-soluble components and recovering the residual solids; (iv) extracting alginate from the residual solids and recovering a residual wet residue comprising cellulose fibres and a residue of alginate; and (v) de-watering the residual wet residue whereby to provide said cellulose / alginate-containing material as a dry residue.

The invention relates to the field of microbial fermentation, and provides a method for producing alginate oligosaccharide by fermenting bacillus coagulans by using kelp as a main carbon source. A specific bacillus coagulans strain is screened, rich algin in kelp is used as a main carbon source for fermentation, and an efficient fermentation process is carried out. In the fermentation process, the bacillus coagulans can effectively degrade algin and synthesize alginate oligosaccharide with biological activity. The fermentation method provided by the invention not only improves the yield of alginate oligosaccharide, but also optimizes the fermentation conditions, so that the whole process is more environment-friendly and economic. In addition, the prepared alginate oligosaccharide has wide application potential in the fields of animal husbandry, food, medicine and the like. The invention provides an innovative alginate oligosaccharide production technology which has an important application value.

The present invention provides a method for providing a gelling effect from brown algae by applying mild conditions.

The invention discloses a dried seaweed floss product derived from waste undaria pinnatifida and a preparation method of the dried seaweed floss product, and belongs to the technical field of seaweed food processing. According to the method, waste brown algae raw materials such as stems, old leaves and leftover materials generated in the undaria pinnatifida processing process are utilized, pretreatment, vacuum freeze-drying and smashing are combined, and particularly cellulase and pectinase are adopted for compound enzyme structure softening, so that a loose, fluffy, light and crisp seaweed floss structure is prepared. The low-value part of the undaria pinnatifida, which is originally difficult to utilize, is converted into high-value-added food. A new product form is provided for deep processing of the undaria pinnatifida, and the undaria pinnatifida has high industrialization and market application prospects.

The invention belongs to the technical field of microorganisms, and particularly relates to a microvesicle bacterium capable of degrading sargassum horneri and application of the microvesicle bacterium. The microvesicles are microvesicles okinawensis, the microvesicles are preserved in the China General Microbiological Culture Collection Center on October 27, 2025, and the preservation number of the microvesicles is CGMCC (China General Microbiological Culture Collection Center) No. 36355. The microvesicle bacterium of the sargassum horneri component degrading enzyme is screened from rotten sargassum horneri, cell walls of the sargassum horneri can be efficiently cracked, release of effective components in brown algae is promoted, and therefore utilization of sargassum horneri resources is promoted.

The present application relates to a kind of mediated zymolytic enzyme of brown algae and application of double transcription unit recombinant bacteria of oxyprotein and brown algae, belong to genetic engineering technical field, the recombinant engineering bacteria is composed of two transcription units, one transcription unit is GAP-alpha-Vgb-AOX1TT, another transcription unit is AOX-alpha-102C300C-AOX1TT, two transcription units are expressed in tandem in pPICZ alphaA plasmid.The present application also provides the preparation method and application of the recombinant bacteria.The recombinant bacteria of the present application can improve the secretion expression efficiency of brown algae zymolytic enzyme, especially can be expressed in low-oxygen environment.

This invention relates to the field of feed additive technology, specifically to a compound plant-seaweed feed additive for reducing methane emissions in ruminants and its preparation method. It comprises the following raw materials in parts by weight: 40-60 parts sorghum husk powder, 30-50 parts purple sweet potato powder, 0.5-5 parts functional additives, 5-10 parts red algae, 5-10 parts brown algae, 5-12 parts *Lactobacillus plantarum*, 3-8 parts chitosan, 2-6 parts essential oil, 2-5 parts 3-nitrooxypropanol, 10-15 parts *Scutellaria baicalensis*, 8-12 parts *Codonopsis pilosula*, 3-8 parts *Isatis indigotica*, and 5-18 parts modified zeolite. Through the regulation of *Lactobacillus plantarum* and chitosan, the energy originally used to generate methane is converted into propionic acid. Propionic acid is a major gluconeogenic precursor in ruminants, which is directly converted into glucose for milk production or muscle growth.

The utility model provides a brown algae collecting device, which relates to the technical field of brown algae collection and comprises a telescopic cylinder, a mounting plate, a cutting component, a mounting rod, a collecting frame, a water leakage hole, a filtering piece, a limiting component and a suction pump, according to the device, after cutting of the brown algae is automatically completed, the cut brown algae and water flow are guided to the area of the collecting frame together, then water can be injected into the water storage cavity through the filtering piece and the liquid leakage holes in the collecting frame and finally is introduced into the lake again through the suction pump, and the brown algae and hard impurities in the water can be intercepted; furthermore, the probability that the suction pump is damaged by hard substances in the water is remarkably reduced, and the brown algae collecting procedure is better.

This application relates to the field of microbial fertilizer technology, specifically to a microbial suspension fertilizer based on seaweed nano-zinc oxide, its preparation method, and its application. First, *Sargassum fusiforme* (seaweed) is washed, dried, and ground into powder, then mixed with water to form an algae slurry. Alginic acid lyase is added to the *Sargassum fusiforme* slurry to obtain an enzymatic hydrolysate. Then, the *Sargassum fusiforme* enzymatic hydrolysate is mixed with zinc acetate under heating conditions. The reaction product is centrifuged, washed, and calcined to obtain seaweed nano-zinc oxide particles. Finally, the nano-zinc oxide particles and adjuvants such as compound microbial agents are formulated into a microbial suspension fertilizer. By utilizing the reaction between *Sargassum fusiforme* enzymatic hydrolysate and zinc acetate, seaweed nano-zinc oxide with more uniform particle size can be obtained, and its photocatalytic and antibacterial properties are improved. Furthermore, the microbial suspension fertilizer prepared based on this seaweed nano-zinc oxide can promote crop growth, improve crop quality, and fortify grain zinc nutrition, making it suitable for various agricultural production applications.

The invention relates to the technical field of cyclina sinensis sperm preservation, in particular to a cyclina sinensis sperm freezing preservation method. The cyclina sinensis sperm freezing preservation method comprises the following steps: (1) putting parent shellfish into a seawater culture pond, and accelerating ripening for 7-10 days by using a ripening agent; the ripening agent is prepared from a spirulina extract, a radix paeoniae alba extract, a codonopsis pilosula extract, astaxanthin and alginic acid; (2) controlling 0.05-0.15 m / s water flow to scour and stimulate the cyclina sinensis, selecting individuals with sperms discharged after the parent shellfish gametes start to be discharged, and collecting the sperms of the cyclina sinensis; and (3) mixing the cyclina sinensis sperms with a cryoprotectant, pre-cooling, and then cryopreserving for a long time. Research results show that the use of the cryoprotectant can significantly reduce the degree of DNA damage, and the protection effect may be realized through a plurality of mechanisms such as direct combination of trehalose with DNA molecules, free radical removal, maintenance of the stability of a chromatin structure and the like.

The invention relates to a biostimulant composition containing an extract of brown algae and its use for improving plant growth by stimulating the growth of favorable microorganisms present in soils.

Apparatus, methods, and compositions including fibrous material and seaweed wherein the device has a surface for engaging with substrate and has a rigidity that decreases over time upon exposure to water, substrate, earth, or a combination thereof. The device may be a pot, disk, or sheet and may have a second surface to engage with earth. The fibrous material may be present in 70 weight percent or more or 60 to 97 weight percent. The seaweed may be present in 30 weight percent or less. The seaweed may include a brown macroalgae species and may include intact seaweed, derivatives of seaweed, seaweed processing waste, or a combination thereof. Methods and compositions including forming a slurry of seaweed and fibrous material, molding the slurry into the device, and drying the device. Forming the slurry may include hydrating fibrous material, seaweed, or both or introducing fibrous material to an agitation vessel.

A C-terminal truncated alginate lyase and its application. This invention belongs to the fields of bioengineering, genetic engineering, and enzyme engineering, and relates to a C-terminal truncated alginate lyase TY2 designed with molecular docking assistance, its encoding gene, recombinant vector, strain, and its application in the preparation of low-polymerization degree alginate oligosaccharides. Using wild-type MhAly6 as the parent, the truncation boundary was determined through structural prediction and molecular docking. A portion of the linker peptide was retained, and the C-terminal GH28 family domain was deleted to obtain the truncated form TY2. TY2 has approximately twice the specific activity of the wild-type enzyme, and even higher residual activity after incubation at 45°C for 1 hour, achieving a synergistic improvement in catalytic activity and thermal stability. + The relative enzyme activity reached 4.3 times that of the control group, which can degrade pretreated Sargassum powder. The product is mainly ΔDP2–ΔDP4 low-polymerization brown algae oligosaccharides, which are suitable for related enzymatic degradation and oligosaccharide preparation.