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Preparation method of brown algae oligosaccharide monomers and brown algae oligosaccharide

A brown algae oligosaccharide and monomer technology, applied in the field of food processing, can solve the problems of increasing the difficulty of separation and purification of sugar monomers and the like

Active Publication Date: 2017-05-31
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are not only guluronic acid oligosaccharides but also mannuronic acid oligosaccharides in the enzymatic hydrolysis products of brown algae; even the same type of oligosaccharides are composed of sugar monomers with different chain lengths and very similar physical and chemical properties. Complexity increases the difficulty of separation and purification of sugar monomers

Method used

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  • Preparation method of brown algae oligosaccharide monomers and brown algae oligosaccharide
  • Preparation method of brown algae oligosaccharide monomers and brown algae oligosaccharide
  • Preparation method of brown algae oligosaccharide monomers and brown algae oligosaccharide

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preparation example Construction

[0038] Such as figure 1 Shown, the preparation method of the fucoidan oligosaccharide monomer of one embodiment, comprises the following steps:

[0039] S10. Using enzymatic hydrolysis to degrade brown algae or alginate to obtain an enzymatic hydrolysis solution, and

[0040] S20. Separating and purifying the enzymolysis solution to obtain the fucoidan oligosaccharide monomers; the fucoidan oligosaccharide monomers include mannuronodisaccharides and mannuronotrioses.

[0041] The preparation method of the above-mentioned fucoidan oligosaccharide monomers uses enzymatic hydrolysis to degrade brown algae or alginate to obtain an enzymolysis solution, and then separates and purifies the enzymolysis solution to obtain fucoidan oligosaccharides including mannuronic acid disaccharides and mannuronic acid trisaccharides. sugar monomers.

[0042] see figure 2 , step S10, using an enzymatic method to degrade brown algae or alginate to obtain an enzymatic solution, comprising the fo...

Embodiment 1

[0066] 1. Preparation of Crude Enzyme Solution

[0067] Take the Bacillus saccharophilus YIC-Alg 3 cryopreservation tube at -80°C, thaw naturally at room temperature, inoculate it in 100ml seawater medium in an ultra-clean bench, and culture it in a shaker at 30°C at 200rpm for 12h.

[0068] Take 4ml of the above-mentioned bacterial solution, inoculate it in 200ml of modified Alg medium, culture at 30°C for 30h, and obtain a fermentation broth. The formula of the improved Alg medium is 20g sodium alginate, 5g ammonium sulfate, 1g magnesium sulfate, 2g dipotassium hydrogen phosphate, add deionized water to 1000ml, pH 7.2-7.4.

[0069] Centrifuge at 12000r / min for 15min to remove the bacteria, take the supernatant and add ammonium sulfate to 80% saturation, put it in a refrigerator at 4°C for 12h to fully precipitate the sodium alginate lyase, and then store it at 4°C Centrifuge for 15 minutes to save the precipitate. After the precipitate is fully dissolved with 1.5ml of phos...

Embodiment 2

[0081] 1. Preparation of Crude Enzyme Solution

[0082] Take the Bacillus saccharophilus YIC-Alg 3 cryopreservation tube at -80°C, thaw naturally at room temperature, inoculate it in 100ml seawater medium in an ultra-clean bench, and culture it in a shaker at 30°C at 200rpm for 12h.

[0083] Take 4ml of the above bacterial solution, inoculate it in 200ml of Alg medium, incubate at 30°C for 24h to obtain a fermentation broth, centrifuge at 12000r / min for 15min to remove the bacteria, add ammonium sulfate to the supernatant until the saturation is 80%, put it in 4°C Stand in the refrigerator for 12 hours to fully precipitate the sodium alginate lyase, and then centrifuge at 4°C for 15 minutes to retain the precipitate. After the precipitate is fully dissolved with 1.5ml of phosphate buffer (pH=7.2), it becomes the crude enzyme solution.

[0084] 2. Preparation of enzymatic hydrolysis solution

[0085] Add 1ml of crude enzyme solution to 40ml of brown algae slurry with a concen...

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Abstract

The invention provides a preparation method of brown algae oligosaccharide monomers. The method comprises the following steps: degrading brown algae or brown algae colloid by using an enzymolysis method to obtain an enzymolysis solution, separating and purifying the enzymolysis solution to obtain the brown algae oligosaccharide monomers such as dimannuronic acid disodium salt and trimannuronic acid trisodium salt and the like. The method is characterized by degrading the brown algae by using the enzymolysis method; compared with the conventional chemical method, the method is mild in condition, clean and environment-friendly in preparation process, free of addition and residue of harmful chemical materials and simple in process.

Description

technical field [0001] The invention relates to the field of food processing, in particular to a preparation method of a fucoidan oligosaccharide monomer. Background technique [0002] Fucoidan oligosaccharide is an oligomer of alginate, which has very important application value in the fields of agriculture, food and medicine. Studies have shown that, in terms of plants, the mixture of fucoidan oligosaccharides can regulate the growth functions of various plants, prolong the life cycle of plants, enhance the immune function of plants, and have great significance in terms of plant yield increase and disease resistance. In animals, algal oligosaccharides can promote the growth of various animals, increase the number of immune cells in various animals, reduce the number of harmful bacteria in the digestive tract and animal products, and have a certain role in promoting and regulating the growth and proliferation of cells . It is of great significance in increasing animal pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/12C12P19/04C12P19/00C07H3/04C07H3/06C12R1/07
CPCC07H3/04C07H3/06C12P19/00C12P19/04C12P19/12
Inventor 李莉莉秦松翟诗翔刘正一焦绪栋
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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