680 results about "Dipotassium hydrogen phosphate" patented technology

The invention relates to a novel method for extracting anthocyanin in bilberry by double water phases, and belongs to the technical field of biological engineering. The method is characterized by comprising the following steps: adding inorganic salt solution and hydrophilic low-molecular organic substance into bilberry fruit residue, stirring the mixture evenly, keeping the mixture standing, layering the mixture, extracting the anthocyanin to an upper phase, and distributing the residue at the lower phase or between the two phases so as to achieve the aim of extracting the anthocyanin from the bilberry fruit residue. The inorganic salt is one or a combination of sodium chloride, ammonium sulphate, sodium sulfate, sodium carbonate, potassium carbonate, potassium phosphate, monopotassium phosphate and dipotassium hydrogen phosphate, preferably ammonium sulphate; and the hydrophilic low-molecular organic substance is one or a combination of methanol, ethanol, normal propyl alcohol, isopropyl alcohol, normal butanol, isobutyl alcohol, glycol and acetone, preferably ethanol. The method has the advantages that a novel method for extracting the anthocyanin is developed, the method is easy to industrialize, the utilization ratio of the bilberry is improved, and the production cost of the anthocyanin is lowered.

The invention discloses a high-concentration major element liquid water-soluble fertilizer and a preparation method thereof. The fertilizer comprises the materials in parts by weight: 7-10 parts of dipotassium hydrogen phosphate, 18-20 parts of urea, 8-11 parts of potassium dihydrogen phosphate, 5-8 parts of potassium permangenate, 24-28 parts of potassium formate, 1-3 parts of EDTA-Zn, 0.5-2 parts of instant boron, and the balance water. The method comprises the steps: under the ordinary pressure and in a reaction kettle, firstly stirring potassium permangenate, potassium formate and potassium dihydrogen phosphate for 2 h according to the formula ratio under the condition of the temperature of 80 DEG C, adjusting the temperature to 45 DEG C, stirring for 0.5 h, then dissolving dipotassium hydrogen phosphate and urea in the solution according to the set formula, after the materials are dissolved completely, adding a proper amount of instant boron and EDTA-Zn, heating to dissolve, after reaction dissolution of the materials according to the formula ratio is completed, cooling to the room temperature, packaging, and thus obtaining the major element liquid water-soluble fertilizer product. The process is simple, the raw material cost is low, and the product is suitable for requirements of current dropper devices and can be widely popularized.

A method for preparing solid natto bacillus microecological agent by solid fermenting is disclosed, which belongs to the biological engineering technique field. The strain is separated from natto as natto bacillus which is named as Bacillus natto S703 and the preservation number of the strain is CCTCC M207147. The sieving method comprises the following steps: utilizing the character that the natto bacillus produces natto kinase to do dilute flat separation for natto leach, seeding the obtained single colony in the primary sieving culture medium of the flat which contains fiber, selecting single colony to purify and obtain the fitful natto bacillus according to the size of transparent coil diameter and colony diameter. The strain is inoculated in the solid ferment culture medium which comprises bran, soya bean waste, corn starch, dipotassium hydrogen phosphate and magnesium sulfate, which can produce the natto bacillus microecological agent by solid fermenting, drying and grinding. The prepared natto bacillus microecological agent can be chicken feed, which is provided with obvious modification for daily chicken weighing, feed coefficient and survival rate.

The invention discloses a baicalin metal complex of which the molecular formula is (C21H17O11) xM (H2O) y, wherein M in the formula is Cu (II), x is 1 and y is 2; or M is Fe (III), x is 2 and y is 0; or M is La (III) or Y (III), x is 3 and y is 0. The preparation method comprises the following steps: adding aqueous alkali into baicalin until the baicalin and alkali (sodium bicarbonate, potassium bicarbonate, sodium formate, potassium formate, sodium acetate, potassium acetate, disodium hydrogen phosphate, dipotassium hydrogen phosphate, sodium phosphate or potassium phosphate) are just completely reacted, then adding metal salt (copper salt, ferric salt, lanthanum salt or yttrium salt) for reaction to obtain the baicalin metal complex. The baicalin metal complex has obviously stronger antibacterial and antitumor activities than the baicalin, can be used for preparing antibacterial drugs and antitumor drugs, and has good development and application prospects. In the preparation method, an organic solvent is not used, a strong basic condition is not adopted, and the method is simple and feasible, is green and environment-friendly and has the advantages of low cost, high product purity and high yield.

The invention relates to an aqueous two-phase separation method for total flavonoids, saponins and polysaccharides in astragalus. The method comprises the following steps of smashing an astragalus medicinal material; adding a solvent and performing solid-liquid extraction; filtering and removing impurities to obtain crude extract of the total flavonoids and the saponins of the astragalus; taking and concentrating the crude extract of the astragalus; adding an aqueous two-phase system formed by absolute ethyl alcohol/dipotassium hydrogen phosphate, adding water to make the total amount of the system be a constant value; mixing uniformly and standing, wherein the aqueous two-phase system is divided into an upper phase and a lower phase; taking an ethanol phase which contains a large amount of total flavonoids and saponins of the astragalus out; and concentrating the ethanol phase under a reduced pressure to obtain the extract of the total flavonoids of the astragalus. The method has the beneficial effects that the extraction rate of the total flavonoids and the total saponins of the astragalus; simultaneously the total flavonoids and the total saponins can be separated from the polysaccharides of the astragalus in the crude extract; the used solvent is low in toxicity, favorable to environment friendliness and low in cost; the inactivation or the denaturation of the flavonoids and the saponins of the astragalus cannot be caused; the phase separation time is short; the interfacial tension is low; and residual organic solvent does not exist.

The invention provides a non-flammable hydraulic fluid concentration of water-glycol, comprising the following components on the basis of concentration gross weight: water with 5-30w % content; glycol with 5-30w content; at least one pH value regulator of self-select borax, monopotassium phosphate and dipotassium hydrogen phosphate, with 1-10w% content; at least one C6 to C24 unsaturated fatty acid antiwear agent with 1-15w% content; at least one unctuous agent produced by reaction of oleic acid or stearic acid and diethanolameine or trolamine, with 10-50w% content; at least one antirust of diethanolameine or trolamine, with 10-40w% content, and corrosion inhibitor with 0.5 to 10w% content. The concentration can be mixed into suitable basal liquid to obtain hydraulic fluid composition with good lubricity, antiwear property, noncorrosibility, rust prevention property, oxidation stability and antifoaming property.

The invention relates to a liquid fertilizer used for eggplant based on biogas slurry and its application method, which comprises the following steps by weight: 70-90 parts of biogas slurry, 10-15 parts of ammonium nitrate, 3-5 parts of dipotassium hydrogen phosphate, 5-8 parts of potassium chloride, 1-3 parts of calcium nitrate, 0.8-1.5 parts of ferrous sulphate, 0.5-0.8 parts of magnesium chloride, 0.2-0.5 parts of zinc sulfate, 0.5-1.5 parts of boric acid, 0.3-0.5 parts of manganese sulfate and 0.2-0.5 parts of ammonium molybdate. According to the invention, the biogas slurry which is obtained by anaerobic fermentation of livestock and poultry excrement and crop straw through a methane tank, the nutrient composition required by the growth of the eggplant can be scientifically added to prepare the liquid fertilizer. The liquid fertilizer has the advantages of comprehensive nutrition, sufficient raw material source, low cost and safe environmental protection. The liquid fertilizer is diluted by using clear water, and is taken as an eggplant foliar fertilizer for spraying or a nutrient solution for soilless culture, and can obviously improve the growth property of the eggplant, the incidence of disease is reduced, and the output is increased to more than 14%. The liquid fertilizer solves the problems of difficult application, difficult transportation and environmental pollution due to secondary discharge.

The invention discloses a method for extracting hesperidin from tangerine peel. The method comprises: mixing tangerine peel with a solid-phase alkali reagent, wherein the tangerine peel is tangerine peel powder which is ground to have a powder particle size analysis result D90 between 10 and 200 mu m; adding water, stirring, centrifuging and taking supernatant; adding acid to regulate pH between 2.0 and 6.0; standing and then filtering the obtained product; drying a filter cake; and obtaining hesperidin. The solid-phase alkali reagent is one or arbitrary mixture of more than two of sodium hydroxide, aluminum hydroxide, calcium hydroxide, potassium hydroxide, sodium bicarbonate, potassium bicarbonate, sodium carbonate, potassium carbonate, calcium carbonate, sodium silicate, disodium hydrogen phosphate or dipotassium hydrogen phosphate. By adopting a mechanochemical technique to extract and separate the hesperidin, the method has the advantages of high extraction rate, high product purity, simple process, environment-friendly property, low cost and short production cycle, and is an extraction-separation route with broad industrialization prospects.

The present invention discloses an insecticide-fertilizer particulate agent and a preparation method thereof. The insecticide-fertilizer particulate agent comprises an insecticide, a bactericide, a fertilizer and an auxiliary agent. The insecticide is composed of one or more components selected from diazinon, thiamethoxam, imidacloprid, clothianidin, chlopyrifos, phoxim, carbofuran and carbosulfan. The bactericide is composed of one or more components selected from azoxystrobin, pyraclostrobin, fludioxonil, metalaxyl, methylimidazole bacteria aniline, thifluzamide and mancozeb. The fertilizer is composed of one or more components selected from urea, ammonium sulfate, potassium sulfate, potassium chloride, monoammonium phosphate, diammonium phosphate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, ammonium polyphosphate, potassium nitrate, zinc sulfate, manganese sulfate, magnesium sulfate, borax, boric acid, ferrous sulfate, copper sulfate, ammonium molybdate, ethylene diamine tetraacetic acid, calcium nitrate, potassium humate, organic matter, amino acid and soybean meal. The insecticide-fertilizer particulate agent is applied during the crop sowing period and can be used for preventing and controlling crop underground pests and diseases at the rootstocks of crops.

A calcium and/or nutritional mineral fortified milk or milk powder product utilises pyrophosphates or orthophosphates in combination with maintenance of pH within the range of 6.5 to 7.5 to render the milk heat stable. Additional calcium and/or nutritional mineral is added in soluble form either before or after the phosphate addition. The preferred orthophosphates are one or more of monosodium dihydrogen orthophosphate, disodium hydrogen orthophosphate, trisodium orthophosphate, monopotassium dihydrogen orthophosphate, dipotassium hydrogen orthophosphate and tri potassium orthophosphate. Addition of an alkaline agent to adjust the pH is not needed if an appropriate mix of orthophosphates is used. The milk products or milk products recombined from milk powders are heat stable and do not have the problems of translucency, gritty mouth feel or sedimentation which can be associated with other stabilised fortified milks.

The present invention discloses a method for culturing nostoc paludosum by utilizing biological reactor. Said method includes the following steps: (1) preparing culture liquor, adopting magnesium sulfate, calcium chloride, citric acid, ammonium ferric citrate, disodium oxalate tetracetate, sodium carbonate, trace element liquor A5 and dipotassium hydrogen phosphate to make preparation according to a certain proportion; (2). algae seed preparation, using the culture of spherical body obtained from second-grade or third-grade culture as inoculative seed source; (3). using biological reactor to make culture, controlling temp., light intensity and ventilation condition of culture liquor; (4). preparing inoculum; (5). culture; (6) monitoring; (7) harvesting; (8) raising mechanical strength of nostoc paludosum spherical body; and (9) drying.

The invention especially discloses a method for preparing soluble corn peptide from Aspergillus niger fermented maize yellow powder, which belongs to the technical field of food biochemistry. According to the invention, Aspergillus niger is used in the method, main components of a medium comprise maize yellow powder, wheat bran and water, a proper amount of cane sugar and dipotassium hydrogen phosphate are added into the main components, and the steps of centrifugation, pumping filtration, decolouring, ultrafiltration and gel chromatography are carried out to obtain a soluble corn peptide solution. The method has the advantages of a simple process, low cost and a few by-products and enables the obtained corn peptide to have high water-solubility, good security, etc.

The invention discloses a preparation technique of a photosynthetic bacteria reagent, which comprises rhodopseudomonas palustris strain cultivation steps that include activated strain cultivation with an inclined plane, strain cultivation with a shaking table, cultivation with an aerated fermenting jar and the like, and adopts the culture medium comprising a basic culture medium and a growth promoting agent. The basic culture medium comprises following components by mass: 0.9 to 1.1 percent of glucose, 0.04 to 0.06 percent of carbamide, 0.02 to 0.03 percent of dipotassium hydrogen phosphate, 0.02 to 0.03 percent of monopotassium phosphate, 0.02 to 0.03 percent of magnesium sulfate, 0.20 to 0.30 percent of sodium chloride, 0.03 to 0.05 percent of calcium chloride and 98.40 to 98.77 percent of water. The added growth promoting agent is 0.10 to 0.30 percent of fulvic acid by mass referring to the basic culture medium. The photosynthetic bacteria reagent that is obtained by the preparation technique can reach the lysate concentration of 4000 to 6000 million per milliliter, and can be used in multiple fields of aquiculture, wastewater processing, crop production and the like.

The invention discloses Chicken semen diluent as well as a preparation method and application thereof, and belongs to the technical field of animal breeding. Chicken semen is diluted for normal temperature preservation and artificial insemination. The Chicken semen diluent takes sterile distilled water as a solvent and contains fructose, citric acid, sodium glutamate, potassium chloride, dipotassium hydrogen phosphate, Tris, bovine serum albumin, VB12 (Vitamin B12), streptomycin and penicillin. The preparation method comprises the following steps of: dissolving the fructose, citric acid, sodium glutamate, potassium chloride, dipotassium hydrogen phosphate, Tris, bovine serum albumin and VB12 into a small amount of sterile distilled water under the sterile condition, adding the penicillin and the streptomycin, adding the sterile distilled water, mixing uniformly to obtain the Chicken semen diluent, and preserving at the temperature of 4 DEG C; and preheating the Chicken semen diluent to the temperature of 37 DEG C, and mixing the Chicken semen diluent and Chicken semen, wherein the mass ratio of the Chicken semen to the Chicken semen diluent is (1:1)-(1:2). The Chicken semen diluent has the advantages of simplicity and convenience in preparation, convenience in material acquisition, low cost and easiness in popularization.

The invention discloses a liquid leaf fertilizer and manufacturing method, which comprises the following parts: 230g solid polyammonium phosphoric acid, 300g dipotassium hydrogen phosphate with 3 crystal water (k2HPO4 .3H2O), 20g aquacare, 430g water, 12g octal-sodium boric kernite, 5g zinc sulphate heptahydrate and 3g sodium nitride solution.

The invention relates to a kind of Bacillus mucilaginosus and a culture method and culture medium thereof, belonging to the microbiological technical field. The Bacillus mucilaginosus P.muc3016 has the functions of nitrogen fixation, phosphorus-dissolving and potassium-releasing and belongs to the Paenibacillus group. The batch fermentation method is adopted to ferment and culture the Bacillus mucilaginosus and the concentrations of carbon source and dissolved oxygen are monitored and regulated. The culture medium contains carbon source, soybean meal powder, ammonium sulfate, magnesium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, calcium sulfate and calcium carbonate, and the pH value of the culture medium is 7.5-7.0. The Bacillus mucilaginosus of the invention has three functions of nitrogen fixation, phosphorus-dissolving and potassium-releasing simultaneously, has higher function activity and can obviously increase the crop yield; and by adopting the culture method and culture medium of the invention, the fermentation density can be increased and the fermentation time can be shortened.

Belonging to the technical field of morchella cultivation, the invention discloses a morchella substrate formula, which is prepared from the following components by weight percentage: 35-45% of wheat hull, 30-35% of sawdust, 15-25% of oat, 5-10% of humus soil, 1-2% of gypsum, and 0.1-0.5% of dipotassium hydrogen phosphate. The formula adopts wheat hull, sawdust, oat, humus soil and other cheap raw materials as the main components of the morchella substrate, and has the characteristics of sufficient raw material sources, low production cost and economic cost saving. The invention also discloses a method for cultivation of morchella with the morchella substrate, the cultivation method is close to natural environment growth, and lays the foundation for high and stable yield of morchella.

The invention relates to a wettable powder of bacillus methylotrophicus. The wettable powder is composed of raw powder of bacillus methylotrophicus that is prepared with a specific method, light calcium carbonate, sodium dodecyl benzene sulfonate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate and 2-3 parts by weight of a dispersant methylene dimethyl naphthalene sodium sulfonate. Furthermore, the invention relates to a use of the wettable powder in controlling citrus diseases, in particular citrus canker.

The process of preparing liquid compound microbial fertilizer belongs to the field of agricultural biotechnology. The preparation process includes inoculating beer yeast, plant lactobacillus and Bacillus lacticus separately to produce liquid bacteria seeds in conical flasks; mixing potato, brown sugar, glucose, peptone, magnesium sulfate, manganese sulfate, dipotassium hydrogen phosphate and sodium chloride in certain weight proportion; dissolving the mixture and heat treatment to sterilize; inoculating beer yeast, plant lactobacillus and Bacillus lacticus successively; gathering after pH value is lowered to 3.5-4.5; and abacterial packing. The liquid compound microbial fertilizer is acid brown liquid with live bacteria content over 1 billion/ml, and may be used as microbial soil improver and as foliage fertilizer to promote plant growth and raise plant resistance.

The invention discloses a fermentation medium for exracting astragalus polysaccharides. The fermentation medium is prepared from the following compositions in parts by weight: 3.908-6.408 parts of whey powder, 0.445-0.467 parts of peptone, 0.04-0.2 parts of glucose, 0.102-0.522 parts of yeast powder, 0.142-0.246 parts of inorganic salt, 12.54-19.46 parts of astragalus powder and 200 parts of water, wherein the inorganic salt comprises potassium dihydrogen phosphate and dipotassium hydrogen phosphate. The fermentation medium for exracting astragalus polysaccharides, disclosed by the invention, can be used for obviously and stably improving the extraction ratio of astragalus polysaccharides and ensuring that the content of astragalus polysaccharides in extraction products is obviously improved.

The invention relates to a high-yield bacterial cellulose fermentation medium and a fermentation method of bacterial cellulose and solves the present problem that the yield of bacterial cellulose is low. The fermentation medium consists of glucose, beef extract, yeast extract, disodium hydrogen phosphate, dipotassium hydrogen phosphate, ethanol, sodium citrate and distilled water. The fermentation method comprises the following steps: 1) activating strains; 2) culturing seeds; and 3) performing fermentation culture. By adopting the technology of the invention, the yield of bacterial cellulose is greatly increased and the average increase of the yield is more than 80%. The high-yield bacterial cellulose fermentation medium and the fermentation method of bacterial cellulose can be used in the production field of bacterial cellulose.

The invention relates to a biological detoxification method for camellia seed cakes. According to the invention, dipotassium hydrogen phosphate, magnesium chloride, 2000-4000U/g of acidic cellulase, microbe strains, and the like are added to crushed camellia seed cake; the mixture is well mixed by stirring, and is subject to a reaction for 10-15 days under a temperature of 40-50 DEG C under the effects of both the microbes and the acidic cellulase; an obtained material is dried by baking, and is crushed, such that a finished product is obtained. Tea saponin content in the obtained product is lower than a safety index of a feedstuff standard, and the nutritive value of the product is higher than non-fermented camellia seed cakes. Protein content of the product reaches 15-20%, which is improved by more than 5%. Free amino acid content of the product is higher than 5%, which is improved by more than 25%. Reducing sugar content in the product is higher than 5%, saponin content in the product is lower than 1.0%, and a detoxification rate of the product is higher than 90%. The product can be directly used as a feedstuff, such that a problem of camellia seed cake intoxication in prior art is solved. The production method is advantaged in simple technology and good effect. With the method, non-toxic camellia seed cakes satisfying the requirements of the feedstuff standard can be provided.