105 results about "Dipotassium hydrogen phosphate" patented technology

The invention relates to the technical field of daily chemicals, in particular to a preparation method and application of a sapindus mukorossi fermentation product with oil-controlling and dandruff-removing effects. The preparation method comprises the following steps: (1) drying soapberry in the air, separately powdering peel and seeds, adding 75-80 parts of water, performing ultrasonic extraction at 35 DEG C for 2 hours, then adding 0.5-0.9 part of dipotassium phosphate, sterilizing, and inoculating 3-5% of lactic acid bacteria liquid for fermentation; (2) after the fermentation is finished, adding 3-7 parts of sterilized aloe extract, 2-5 parts of mulberry leaf extract, 5-10 parts of tea bran extract and 6-9 parts of rose extract, adjusting the pH value to 5-7, inoculating 3-5% of schizosaccharomyces pombe, culturing for 24-36 hours at 28-30 DEG C, and introducing oxygen at 0.3-0.5 L / min; and (3) centrifuging at 10000r, and filtering through a sieve plate to obtain clear fermentation liquor, namely the sapindus mukorossi fermented product. The soapberry peel and the soapberry seeds are separately crushed, and the peel powder and the seed powder are mixed according to a ratio of 90%: 10% for use, so that the saponin content and the irritation are favorably controlled, and the use feeling is improved.

The invention relates to the technical field of bacterium enrichment nutrient solutions, in particular to a bacterium enrichment nutrient solution for fermentation and a preparation method of the bacterium enrichment nutrient solution. The culture medium is prepared from the following raw materials in parts by weight: maltodextrin, crystalline glucose, low-pigment yeast extract powder, refined soy peptone, dipotassium phosphate, monopotassium phosphate, magnesium sulfate heptahydrate, sodium chloride, phytic acid, L-ascorbic acid, manganese sulfate monohydrate, biotin, thiamine hydrochloride and choline chloride. Through a double-carbon-source structure, maltodextrin and crystalline glucose cooperate, metabolic oscillation is avoided, and the energy utilization rate is increased; the low-pigment yeast extract powder and the refined soy peptone are complementary, so that the rapid growth requirement of thalli is met; phytic acid is chelated with metal ions to prevent precipitation, L-ascorbic acid is used for resisting oxidation, and biotin and thiamine activate a coenzyme pathway to realize accurate regulation and control of a metabolic network.

The invention belongs to the technical field of microbial culture, and particularly relates to a culture medium composition and application thereof in PDRN recombinant escherichia coli fermentation. The culture medium composition comprises a fermentation culture medium and a fed-batch culture medium, the fermentation culture medium consists of disodium hydrogen phosphate, monopotassium phosphate, ammonium sulfate, magnesium chloride, calcium nitrate tetrahydrate, glycerol and water; the supplemented culture medium is composed of dipotassium phosphate, sodium dihydrogen phosphate, sodium sulfate, ammonium sulfate, ammonium chloride, diammonium hydrogen citrate, glycerin and water, and the metabolic requirements of PDRN recombinant escherichia coli are accurately matched. Meanwhile, the invention provides a fermentation method using the composition, and the problems of unbalanced nutrition, low fermentation efficiency and the like of an existing culture medium are solved. Stable proliferation of thalli can be remarkably promoted, the yield of PDRN products is increased, the culture medium is easy and convenient to prepare, fermentation conditions are easy to control, and the culture medium is suitable for industrial large-scale production and has important application value.

The invention relates to the technical field of enzyme preparations, in particular to an agricultural biological compound enzyme preparation and a preparation method thereof.The agricultural biological compound enzyme preparation is prepared from, by weight, 30-50 parts of compound enzyme, 5-8 parts of trehalose, 3-5 parts of glycerin, 15-20 parts of copolymer carrier, 5-8 parts of humic acid, 5-10 parts of bran, 3-5 parts of dipotassium phosphate and 85-124 parts of water. The compound enzyme contains various enzymes such as cellulase, lysozyme and protease, can synchronously decompose various organic substances such as cellulose, microbial cell walls and protein in soil and release nutrients such as nitrogen, phosphorus and potassium, has more comprehensive functions compared with a single enzyme preparation, and can meet the requirements of complex soil and crops in agricultural production.

The invention belongs to the technical field of polyether synthesis, and discloses a synthesis method of dimethyl-terminated polyether. The synthesis method comprises the following steps: mixing methanol polyether with an alkoxide reagent, heating and degassing, then adding an adsorbent and a seed crystal, then adding chloromethane to carry out a curing reaction, and finally carrying out secondary degassing to prepare the dimethyl-terminated polyether, wherein the seed crystal is selected from at least one of disodium hydrogen phosphate, sodium dihydrogen phosphate, dipotassium phosphate and potassium dihydrogen phosphate; the adding amount of the seed crystal is 0.1%-2% of the mass of the methanol polyether. According to the synthesis method provided by the invention, a synthesis process and a refining process are combined, and the total process time can be greatly shortened, the preparation efficiency can be improved and the production cost can be reduced by controlling the adding time of the adsorbent and the crystal and cooperatively using the adsorbent and the crystal; and moreover, the quality of the dimethyl-terminated polyether can be improved, and the prepared dimethyl-terminated polyether finished product is light in color, low in hydroxyl value, low in potassium and sodium ion content and high in termination rate.

The invention belongs to the technical field of aquaculture, and particularly relates to a feed additive for aquaculture as well as a preparation method and application of the feed additive. The preparation method of the feed additive comprises the following steps: (1) weighing the raw material medicines, adding water for soaking, boiling with strong fire, then decocting with slow fire, and collecting first filtrate and first filter residues; (2) adding water into the first filter residue, boiling with strong fire, decocting with slow fire, collecting a second filtrate, and concentrating to obtain a traditional Chinese medicine composition extracting solution; (3) adding yeast powder, peptone, dipotassium phosphate, inositol galactoside and 1, 3-diphosphoglyceric acid into the traditional Chinese medicine composition extracting solution to obtain a fermentation culture medium; and (4) inoculating the lactobacillus fermentum liquid and the bacillus subtilis liquid into the fermentation culture medium for fermentation to obtain a fermentation mixture, and performing centrifugation and freeze drying to obtain the lactobacillus fermentum and bacillus subtilis compound feed. The feed additive for aquaculture is added into feed, so that the growth and development capability of animals can be effectively promoted, the immunocompetence of the animals is improved, and the feed additive has a wide application prospect.

This invention relates to the field of microbial fertilizer technology, specifically to a microbial compound phosphorus and potassium fertilizer that promotes nutrient absorption and its preparation method. The preparation method of a microbial compound phosphorus and potassium fertilizer that promotes nutrient absorption includes: preparing sunflower disc extract and Moringa leaf extract; preparing slow-release microcapsules; preparing microspheres loaded with bacteria; and preparing the compound phosphorus and potassium fertilizer. This invention first selectively oxidizes konjac glucomannan using sodium periodate, then crosslinks the oxidized konjac glucomannan with lignin under weakly acidic conditions to form a three-dimensional network crosslinked polymer framework. Urea and dipotassium hydrogen phosphate are encapsulated in the network channels or adsorbed onto the framework during the crosslinking process, forming nutrient-loaded slow-release microcapsules. After the slow-release microcapsules are made into a compound phosphorus and potassium fertilizer and applied, nutrients are gradually released as the crop grows, which helps improve nutrient absorption and utilization, thereby promoting nutrient absorption.

The invention relates to the technical field of plant essential oil extraction, and discloses a valerian-rich rhizoma nardostachyos essential oil directional preparation process, which comprises: taking a functionalized compound solvent, adding rhizoma nardostachyos coarse powder according to a liquid-solid ratio, stirring and soaking to obtain a solid-liquid mixture; the solvent component comprises process water, sodium xylene sulfonate, dipotassium phosphate and polyethylene glycol 400; introducing steam into the mixture, heating to maintain slight boiling, carrying out first-stage distillation, and removing distilled low-boiling-point components to obtain resident in the kettle; cooling the resident to a constant temperature, adding sodium chloride, and stirring until the sodium chloride is dissolved to obtain a heterogeneous mixed system; the heating system is subjected to second-stage distillation, and distillate is collected; and carrying out oil-water separation on the distillate, and collecting an oil phase to obtain a finished product of the rhizoma nardostachyos essential oil. According to the method disclosed by the invention, a hydrotropy-salting-out phase change mechanism is matched with fractional distillation, so that high-efficiency desorption and directional enrichment of valerianone which is difficult to volatilize are realized while acidic and light component impurities are removed.

The application discloses a preparation method of glutamic acid bacterial protein ecological fertilizer, and the preparation steps are as follows: firstly, zinc polysilicate is added into glutamic acid fermentation waste liquid, and bacterial protein-zinc polysilicate complex is obtained through filtration; then, zinc silicate and hydroxyapatite are added, and amino acid composite hydroxyapatite particles are formed through the action of citric acid; and finally, urea-humic acid-dipotassium hydrogen phosphate complex is sprayed on the amino acid composite hydroxyapatite particles to obtain the ecological fertilizer. The ecological fertilizer can not only fully utilize the bacterial protein in the glutamic acid fermentation waste liquid, but also play a good slow-release effect of nitrogen, phosphorus and other elements through the action of two complexes. In addition, the addition of silicon and zinc elements can play a supplementing effect for the growth of crops, so that the fertilizer has the dual functions of environmental protection and long-acting slow release, and has a good promoting effect on the growth of crops.

The present disclosure provides a reagent and a method for detecting paraquat in herbicide preparation, the reagent comprising reagent A, reagent B and reagent C, the reagent A being a solution prepared from dipotassium hydrogen phosphate and an alkaline buffer, the reagent B being methanol, and the reagent C being a solution of acetylphenylhydrazine. The method comprises mixing the herbicide preparation to be tested with the reagent A to form a uniform solution A, then adding the reagent B to the uniform solution A, shaking and waiting for phase separation, taking the supernatant B and adding it to the reagent C, immediately visually comparing the color or using a spectrophotometer to compare the color, and determining whether the herbicide preparation to be tested contains paraquat according to the color change. The present disclosure uses acetylphenylhydrazine as a reducing agent combined with solvent to control the redox potential of the reducing agent, develops a detection system specific for reducing paraquat, and applies it to the detection of latent ingredients in herbicide preparations, which has the characteristics of high detection sensitivity, convenient operation and obvious phenomenon, and is suitable for on-site detection.

The application discloses a chromogenic culture medium for detecting enteropathogenic Escherichia coli, which contains peptone, sodium pyruvate, sodium chloride, D-sorbitol, D-malic acid, bile salt, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, sodium hydroxide, kaolin, bromothymol blue, crystal violet, polyethylene glycol and agar, and the rest is water. The chromogenic culture medium is simple in composition, convenient to prepare and use, and can be used for identifying enteropathogenic Escherichia coli EPEC according to the color of bacterial colonies, and has the advantages of specificity, high efficiency, rapidness, low cost, simple result judgment and the like, and has a wide application prospect in food safety monitoring.

The application discloses a kind of genetic engineering escherichia coli plasmid expression culture medium and fermentation process, including basic medium and feed medium;The basic medium includes glycerol, yeast hydrolysate, yeast peptone, L-glutamic acid, L-valine, L-isoleucine, arginine, tyrosine, lysine, proline, glycine, ammonium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, citric acid, magnesium sulfate, ferrous sulfate, zinc sulfate, calcium chloride, manganese chloride, cobalt chloride, copper chloride, sodium ethylenediaminetetraacetate;Feed medium includes glycerol, citric acid, magnesium sulfate, ferrous sulfate, zinc sulfate, calcium chloride, manganese chloride, cobalt chloride, copper chloride, sodium ethylenediaminetetraacetate.The application inhibits the accumulation of harmful metabolites, and creates the optimal intracellular environment for stable replication of plasmid.In industrial fermentation scale, the amount of plasmid harvested per liter of fermentation broth is more than 1 gram, and the supercoiling ratio of plasmid DNA is stably maintained at more than 90%, with minimal batch-to-batch variation.

This invention discloses a culture medium for manganese-oxidizing bacteria suitable for low-carbon source environments in water supply networks and its application. The culture medium is prepared by mixing ferric ammonium citrate, manganese sulfate, ammonium sulfate, sodium chloride, magnesium sulfate, dipotassium hydrogen phosphate, and sodium nitrate, followed by high-temperature and high-pressure sterilization to obtain a culture solution. Manganese sulfate is added to the culture solution to obtain the manganese-oxidizing bacteria culture medium. Manganese-oxidizing bacteria are screened using this culture medium. Cast iron pipe scale is subcultured in the medium to enrich the manganese-oxidizing bacteria culture solution. The enriched manganese-oxidizing bacteria culture solution is diluted and spread onto agar solid medium, and incubated at a constant temperature to obtain manganese-oxidizing bacteria colonies. This invention solves the problem of altered bacterial physiological metabolism caused by high concentrations of added manganese ions, effectively improving bacterial activity and manganese oxidation capacity.

The invention provides an inorganic matter and cationic organic matter compounded clay stabilizer for water injection, and discloses that trimethyl monoallyl ammonium chloride is used as a raw material, and polymerization in the use process is prevented after a p-benzoquinone polymerization inhibitor is added, so that cationic monomer micromolecules containing double bonds are used as an organic clay stabilizer, and the stability of the organic clay stabilizer is improved. When being used together with the same raw material cationic organic matter with small molecular weight, namely 2-hydroxyethyl trimethyl ammonium chloride, the clay swelling prevention agent has the performance of preventing clay swelling, anti-scouring capability and long-term effect, and has better performance of preventing clay swelling after being compounded with cheap inorganic raw materials, namely aluminum chloride, dipotassium phosphate and industrial ammonium chloride; the prepared clay anti-swelling agent compounded by the inorganic matter and the cationic organic matter not only has excellent capacity of preventing clay swelling, but also has good anti-scouring capacity and good washing resistance, and an innovative demonstration effect is achieved for preventing damage of clay swelling and migration to a reservoir in water injection and improving the water injection recovery ratio.

The invention belongs to the technical field of biology, and particularly discloses a chicken semen cryopreservation solution and a preparation method thereof. The chicken semen cryopreservation liquid comprises a cryoprotection liquid I and a cryoprotection liquid II. The freezing protection liquid I is prepared from the following components: glucose, sodium glutamate, potassium acetate, dipotassium phosphate, magnesium acetate, N, N-bis (2-ethoxyl)-2-aminoethanesulfonic acid and an antibiotic solution. And the freezing protection liquid II comprises dimethylformamide and nano zinc oxide. The invention discloses a chicken semen cryopreservation solution and a preparation method thereof, by applying the chicken semen cryopreservation solution, the fertilization and incubation performance of frozen semen is remarkably improved, centrifugation-free and direct semen deposition of the frozen semen are realized, and physical damage and activity reduction caused by the centrifugation process to sperms are avoided.

The invention provides a biochemical composite soil heavy metal pollution fixing and repairing material and a preparation method thereof.The preparation method comprises the steps that biochar is subjected to acid treatment, and activated biochar is obtained; uniformly mixing calcium salt, ferric salt, aluminum salt and deionized water to obtain a mixed salt solution, adding urea to obtain a precursor solution, dipping the activated charcoal in the precursor solution, and then performing hydrothermal reaction to obtain loaded charcoal; mixing and grinding the oyster shell powder and dipotassium phosphate, and calcining at high temperature to obtain activated oyster shell powder; mixing the bacillus subtilis liquid, the activated oyster shell powder and trehalose, oscillating, centrifugally separating, and freeze-drying to obtain the biological composite material. Uniformly mixing the loaded biochar, the biological composite material and a carboxymethyl cellulose solution, granulating, and drying wet granules to obtain the biochemical compound type soil heavy metal pollution fixing and repairing material. According to the prepared fixing and repairing material, the fixing efficiency and adaptability to various heavy metals in soil are improved.

The invention belongs to the technical field of microorganisms, and discloses a high-density fermentation process of vagina-sourced lactobacillus crispatus. The invention discloses a fermentation medium which comprises glucose, tryptone, beef extract, yeast extract, dipotassium phosphate, ammonium citrate, L-cysteine or salt thereof, magnesium sulfate and manganese sulfate. According to the fermentation culture medium provided by the invention, through scientific compounding of a carbon source, a nitrogen source and key factors, the final viable concentration and thallus activity of vagina-derived lactobacillus crispatus can be remarkably improved. The fermentation culture medium is adaptive to the micro-aerobic metabolism characteristics of vagina-derived lactobacillus crispatus by precisely regulating and controlling the dissolved oxygen level (initial dissolved oxygen is controlled to be 5% or below), and when the fermentation culture medium is used for culturing lactobacillus crispatus, the fermentation delay period can be remarkably shortened to 2-3 h, the final viable bacterium yield is greatly increased to 1 * 10 < 10 > CFU / mL or above, and the production cost is reduced. And an efficient and stable technical support is provided for industrial production of the probiotic preparation.

The present invention relates to a microbial composite fertilizer and a fermentation medium thereof, and belongs to the field of microbial technology. The microbial composite fertilizer of the present invention comprises 8-10g of a microbial agent and 40-60g of a non-microbial raw material. The microbial agent is produced from a fermentation medium containing fermented bone residue powder, mulberry linterinary polypeptide, broken yeast powder, carotene, wheat bran powder, chicory polysaccharide, calcium carbonate, and dipotassium hydrogen phosphate. The microbial fertilizer of the present invention adopts an effective combination of microbial agents and inorganic fertilizers, which can fix nitrogen in the air in the soil through nitrogen-fixing organisms, activate the insoluble phosphorus and potassium elements in the soil into a state that can be absorbed and utilized by plants, and improve the physical and chemical properties of the soil to a certain extent; at the same time, the use of the fertilizer can improve the physical properties of the soil, improve the aggregate structure in the soil, increase the voids in the soil, and improve the permeability and water permeability of the soil.

The present invention discloses a slow-release fertilizer, which comprises: (1) fly ash, (2) dipotassium hydrogen phosphate and / or potassium dihydrogen phosphate and / or tripotassium phosphate, and (3) an inorganic base. The slow-release fertilizer is prepared by mixing fly ash, dipotassium hydrogen phosphate and / or potassium dihydrogen phosphate and / or tripotassium phosphate and the inorganic base, calcining at 550-850°C, adding water after cooling, and crystallizing under pressure at a temperature of 110-160°C. The prepared fertilizer P and K nutrient elements are loaded in a porous, high-specific-surface-area microporous molecular sieve, with a high loading amount, slowly released through the environment, and having a long-lasting fertilizer effect. The molecular sieve material has a high specific surface area and cation exchange capacity, and in the presence of Cd 2+ ions, Cr 6+ When used in soil environments with highly toxic heavy metal ions such as ions, it can effectively adsorb heavy metal ions in the environment, control their entry into crops, and protect food health.

The invention relates to the technical field of production of L-arginine, and discloses a fermentation culture method and a fermentation culture medium for a high-yield strain of L-arginine. The influence of strain fermentation conditions on the yield of L-arginine is neglected in the prior art. According to the invention, the maximization of the concentration of L-arginine produced by fermentation is taken as a target, the fermentation temperature is optimized to 33-37 DEG C, and the composition of a fermentation culture medium is optimized into engineering bacteria which are more suitable for being transformed by a metabolic pathway for producing L-arginine, so that the yield of the L-arginine of the strain is greatly improved. Wherein the fermentation culture medium is prepared by selecting a proper carbon source and a proper nitrogen source, adding a proper concentration of sodium citrate and copper sulfate, and adding a proper concentration of L-sodium glutamate, dipotassium phosphate, magnesium sulfate, manganese sulfate, ferrous sulfate and betaine.

The invention discloses a compound medicine composition for preventing osteoporosis of middle-aged and elderly people, and belongs to the technical field of medicines. The composition is composed of calcium carbonate, calcium hydrophosphate, magnesium oxide, ferrous fumarate, dipotassium phosphate, manganese sulfate, zinc oxide, copper sulfate and vitamin D. Through scientific compounding of the calcium source, the vitamin D and various trace elements, all the components have a synergistic effect, so that nutrients required by bones can be comprehensively supplemented, the calcium absorption efficiency is improved, the bone metabolism is improved, the bone strength and toughness are enhanced, the osteoporosis of the middle-aged and elderly people is effectively prevented and relieved, the irritation to gastrointestinal tracts is small, the safety is high, the preparation is simple, and the cost is controllable; the composition is suitable for long-term administration of middle-aged and elderly people, and can be widely applied to the fields of food, health care products or medicines for preventing and treating middle-aged and elderly osteoporosis.

The invention relates to an HPLC (High Performance Liquid Chromatography) high-efficiency quantitative method for methionine and guanidinoacetic acid in a creatine substrate synthesized by whole-cell biological catalysis. According to the method, an amino bonded silica gel chromatographic column is selected, the length of the chromatographic column is 250mm, the inner diameter of the chromatographic column is 4.6 mm, and the particle size of filler is 5mu m. A mobile phase A is 20 mmol of a dipotassium phosphate solution, the pH value is adjusted to 6.0-8.0 by using phosphoric acid, a mobile phase B is acetonitrile, and gradient elution is performed; the detection wavelength is 200 to 210 nm; the column temperature is 25-35 DEG C; the flow velocity is 0.7 to 1.0 mL / min. The detection method can be applied to accurate quantification of methionine and guanidinoacetic acid in a whole-cell biocatalytic synthesis creatine substrate, is simple in sample pretreatment, high in recovery rate, simple to operate, low in detection cost and short in detection time, has high-throughput sample detection capability, and is suitable for large-scale popularization and application. And a favorable tool is provided for exploring a safe and environment-friendly method for synthesizing creatine through industrial whole-cell biological catalysis.

The invention relates to the technical field of medicines, in particular to an intelligent response type cervical auxiliary fertility gel which comprises the following components in percentage by mass: 0.1-2% of a gel matrix which is selected from one or more of hydroxypropyl methyl cellulose, hydroxyethyl cellulose, sodium alginate and gellan gum; 0.05%-0.5% of a gel adjusting auxiliary agent which is selected from zinc chloride or calcium lactate; the osmotic pressure regulator is selected from one or more of glycerol, glucose, sodium chloride, mannitol, sorbitol, polyethylene glycol and propylene glycol; 0.1%-2% of an acid-base regulator which is selected from one or more of disodium hydrogen phosphate, sodium dihydrogen phosphate, dipotassium phosphate and potassium dihydrogen phosphate; according to the present invention, the toxicity grade of the gel on cervical cells is less than or equal to grade 2 (ISO 10993-5 standard), the cell survival rate is more than or equal to 70%, and the safety is high.

The invention discloses acid-resistant neutral modified milk and a preparation method thereof, the modified milk comprises the following components in percentage by mass: 5-65% of water, 20-80% of milk, 5-15% of single cream, 0-6% of milk powder, 2-12% of white granulated sugar, 0.3-0.9% of an emulsifier, 0.1-0.7% of a thickener and 0.5-2.2% of an acidity regulator, the acidity regulator is prepared from 0.3-1% of potassium citrate, 0.1-0.5% of dipotassium phosphate, 0.1-0.5% of sodium citrate, 0.1-0.5% of sodium citrate, 0.1-0.5% of sodium citrate, 0.1-0.5% of sodium citrate, 0.1-0.5% of sodium citrate, 0.1-0.5% of sodium citrate, and the balance of water. The cleaning agent is prepared from the following components in percentage by weight: 0.05 to 0.5 percent of sodium tripolyphosphate, 0.02 to 0.1 percent of sodium hexametaphosphate and The modified milk disclosed by the invention can be placed in fruit milk tea (obvious in sourness) with the pH of more than 4.6 for more than 2 hours at normal temperature without flocculation and layering; and after being placed for more than 1 hour at normal temperature under the acidic condition that the pH value is 3.4-3.8, part of the areas are extremely slightly flocculated, and the state and the taste are not influenced by shaking up.

The invention discloses a chelate-rare earth compound liquid fertilizer and a preparation method thereof, and the chelate-rare earth compound liquid fertilizer comprises the following components in parts by weight: 500-600 parts of pyroligneous liquor, 40-50 parts of monopotassium phosphate, 60-80 parts of dipotassium phosphate, 200-250 parts of chelating agent amine alkyl lignin, 0-50 parts of zinc sulfate, 0-50 parts of ferric sulfate, 0-15 parts of manganese sulfate, 6-12 parts of low-viscosity sodium alginate and 10-25 parts of compound sodium nitrophenolate. 5-15 parts of triacontanol milk powder, 2-8 parts of sodium dodecyl benzene sulfonate, 2-8 parts of carrageenan and 0.5-4 parts of rare earth element chloride. Wherein the chelating agent amine alkyl lignin is obtained by reacting small molecular lignin with caprolactam. According to the chelating-rare earth compound liquid fertilizer, the amino alkyl lignin is adopted to replace a traditional EDTA chelating agent, the chelating capacity with rare metal elements is remarkably improved, meanwhile, the amino alkyl lignin is derived from natural lignin and can be degraded by microorganisms, and the environmental accumulation risk is reduced.

The present application relates to the technical field of culture medium, and discloses a light response type composite culture medium and application thereof in plant cultivation.A carbon fixation microorganism, a nitrogen fixation microorganism and a TiO2 / CdS heterojunction nanomaterial are used to form microspheres, a light nanomaterial and microorganism synergistic carbon fixation and nitrogen fixation system is constructed, and the system is mixed with a buffer solution composed of dipotassium hydrogen phosphate and potassium dihydrogen phosphate to obtain a light response type composite culture medium.The composite culture medium can improve the carbon and nitrogen cycle efficiency through processes such as photocatalysis, hydrogen storage and carbon and nitrogen fixation, and further significantly improve the plant growth cycle and yield.Experiments show that in a closed environment, the composite culture medium provided by the present application can shorten the growth cycle of lettuce to 20 days, and the fresh weight is increased to 60g after 20 days of growth.

The invention discloses an abamectin fermentation culture medium and a fermentation process, and relates to the technical field of culture media. The abamectin fermentation culture medium is prepared from 15 g / L to 30 g / L of glucose, 81 g / L to 103 g / L of dextrin, 8 g / L to 12 g / L of corn steep liquor, 11 g / L to 15 g / L of soybean cake powder, 0.2 g / L to 0.5 g / L of ammonium sulfate, 0.2 g / L to 0.5 g / L of dipotassium phosphate, 0.05 g / L to 0.2 g / L of monopotassium phosphate, 0.05 g / L to 0.32 g / L of manganese sulfate, 0.3 g / L to 0.5 g / L of cobalt chloride, 0.6 g / L to 1.8 g / L of sodium chloride, 0.05 g / L to 0.25 g / L of a defoaming agent and 2.4 g / L to 5.2 g / L of silanization modified aluminum oxide. According to the technical scheme, glucose and dextrin are adopted as carbon sources, corn steep liquor, soybean cake powder and ammonium sulfate are adopted as nitrogen sources, and silanization modified aluminum oxide is added, so that the element production capacity of thalli is improved, and the titer of abamectin B1a is improved.

The chelating type water-soluble fertilizer containing humic acid is prepared from the following raw materials in parts by weight: 35 to 45 parts of urea, 20 to 25 parts of dipotassium phosphate, 20 to 25 parts of calcium ammonium nitrate, 5 to 10 parts of humic acid, 25 to 30 parts of chitosan aqueous solution, 5 to 8 parts of bentonite-doped synergist, 4 to 7 parts of hydroxyapatite regulator, 2 to 4 parts of boric acid, 3 to 5 parts of compound element agent and 2 to 5 parts of vitamin agent. According to the chelating water-soluble fertilizer, the urea, the dipotassium phosphate, the calcium ammonium nitrate and the humic acid are blended, the boric acid, the compound element agent and the vitamin agent are added for mutual blending and regulation, the synergistic agent doped with bentonite and the hydroxyapatite regulator are adopted for regulation and effect regulation, synergistic interaction is achieved jointly, the effect of improving the plant yield and the quality of the product is remarkable, and the yield of the product is increased. Meanwhile, the product acts on the stability of plants under high and cold conditions.

The invention relates to the field of drug sensitivity diagnosis, in particular to a swine-derived broad-spectrum enrichment medium as well as a preparation method and application thereof. The pig-derived broad-spectrum enrichment culture medium is prepared from the following raw materials in parts by weight: 5.0 to 10.0 parts of peptone, 3.0 to 8.0 parts of yeast extract powder, 5.0 to 8.0 parts of sodium chloride, 5.0 to 10.0 parts of lactose, 0.5 to 2.5 parts of cholate, 0.002 to 0.005 part of crystal violet, 0.002 to 0.010 part of neutral red, 15.0 to 20.0 parts of agar, 1.0 to 3.5 parts of glucose, 1.0 to 3.5 parts of dipotassium phosphate, 2.5 to 5.0 parts of disodium hydrogen phosphate, 0.2 to 0.5 part of sodium thiosulfate and 1000 parts of distilled water. 50-100 parts of filtered and sterilized newborn calf serum and 1.0-2.5 parts of NAD (Nicotinamide Adenine Dinucleotide) with the mass fraction of 1%. According to the invention, the formula of the enrichment culture medium is optimized, the liquid culture medium is used as the enrichment culture medium for the drug sensitive test, and a new acid-base indicator is selected according to the biochemical characteristics of bacteria, so that the process of the drug sensitive test is simplified.

The invention relates to a process for extracting lycopene and polysaccharide from tomato pomace and a membrane material prepared from the same, and the process comprises the following steps: S1, uniformly spreading a certain mass of tomato pomace in a dryer for drying, crushing the tomato pomace dried to constant weight, and sieving with a 90-mesh standard sieve to prepare tomato pomace powder; s2, enzyme-assisted aqueous two-phase system extraction: adding the tomato peel residue powder into a disodium hydrogen phosphate-citric acid buffer solution, then adding neutral protease, uniformly mixing, and carrying out enzymolysis in a water bath at 45 DEG C for 60 minutes; and after the enzymolysis reaction is finished, purifying and drying to obtain lycopene and polysaccharide in the tomato pomace. The influence factors of the yield of the lycopene and the polysaccharide in the tomato pomace are explored through a single-factor test, the process conditions for extracting the yield of the lycopene and the polysaccharide are preliminarily obtained by means of an enzyme-assisted aqueous two-phase extraction method, and then the optimal process conditions are obtained through orthogonal test optimization. Finally, the optimal process is determined as follows: the mass fraction of ethyl acetate is 35%, the mass fraction of dipotassium phosphate is 14%, the enzyme activity is 2000U, and the pH value is 7.