17 results about "Dipotassium hydrogen phosphate" patented technology

This invention relates to the field of microbial fertilizer technology, specifically to a microbial compound phosphorus and potassium fertilizer that promotes nutrient absorption and its preparation method. The preparation method of a microbial compound phosphorus and potassium fertilizer that promotes nutrient absorption includes: preparing sunflower disc extract and Moringa leaf extract; preparing slow-release microcapsules; preparing microspheres loaded with bacteria; and preparing the compound phosphorus and potassium fertilizer. This invention first selectively oxidizes konjac glucomannan using sodium periodate, then crosslinks the oxidized konjac glucomannan with lignin under weakly acidic conditions to form a three-dimensional network crosslinked polymer framework. Urea and dipotassium hydrogen phosphate are encapsulated in the network channels or adsorbed onto the framework during the crosslinking process, forming nutrient-loaded slow-release microcapsules. After the slow-release microcapsules are made into a compound phosphorus and potassium fertilizer and applied, nutrients are gradually released as the crop grows, which helps improve nutrient absorption and utilization, thereby promoting nutrient absorption.

The application discloses a kind of genetic engineering escherichia coli plasmid expression culture medium and fermentation process, including basic medium and feed medium;The basic medium includes glycerol, yeast hydrolysate, yeast peptone, L-glutamic acid, L-valine, L-isoleucine, arginine, tyrosine, lysine, proline, glycine, ammonium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, citric acid, magnesium sulfate, ferrous sulfate, zinc sulfate, calcium chloride, manganese chloride, cobalt chloride, copper chloride, sodium ethylenediaminetetraacetate;Feed medium includes glycerol, citric acid, magnesium sulfate, ferrous sulfate, zinc sulfate, calcium chloride, manganese chloride, cobalt chloride, copper chloride, sodium ethylenediaminetetraacetate.The application inhibits the accumulation of harmful metabolites, and creates the optimal intracellular environment for stable replication of plasmid.In industrial fermentation scale, the amount of plasmid harvested per liter of fermentation broth is more than 1 gram, and the supercoiling ratio of plasmid DNA is stably maintained at more than 90%, with minimal batch-to-batch variation.

This invention discloses a culture medium for manganese-oxidizing bacteria suitable for low-carbon source environments in water supply networks and its application. The culture medium is prepared by mixing ferric ammonium citrate, manganese sulfate, ammonium sulfate, sodium chloride, magnesium sulfate, dipotassium hydrogen phosphate, and sodium nitrate, followed by high-temperature and high-pressure sterilization to obtain a culture solution. Manganese sulfate is added to the culture solution to obtain the manganese-oxidizing bacteria culture medium. Manganese-oxidizing bacteria are screened using this culture medium. Cast iron pipe scale is subcultured in the medium to enrich the manganese-oxidizing bacteria culture solution. The enriched manganese-oxidizing bacteria culture solution is diluted and spread onto agar solid medium, and incubated at a constant temperature to obtain manganese-oxidizing bacteria colonies. This invention solves the problem of altered bacterial physiological metabolism caused by high concentrations of added manganese ions, effectively improving bacterial activity and manganese oxidation capacity.

The present application relates to the technical field of culture medium, and discloses a light response type composite culture medium and application thereof in plant cultivation.A carbon fixation microorganism, a nitrogen fixation microorganism and a TiO2 / CdS heterojunction nanomaterial are used to form microspheres, a light nanomaterial and microorganism synergistic carbon fixation and nitrogen fixation system is constructed, and the system is mixed with a buffer solution composed of dipotassium hydrogen phosphate and potassium dihydrogen phosphate to obtain a light response type composite culture medium.The composite culture medium can improve the carbon and nitrogen cycle efficiency through processes such as photocatalysis, hydrogen storage and carbon and nitrogen fixation, and further significantly improve the plant growth cycle and yield.Experiments show that in a closed environment, the composite culture medium provided by the present application can shorten the growth cycle of lettuce to 20 days, and the fresh weight is increased to 60g after 20 days of growth.

This invention provides a kit for the simultaneous detection of nine flavor nucleotides in livestock and poultry products. The flavor nucleotides are cytidine monophosphate, uridine monophosphate, guanylic acid, inosine monophosphate, adenosine monophosphate, hypoxanthine, inosine, adenosine diphosphate, and adenosine triphosphate. The kit includes: a high-concentration extraction reagent, a standard curve solution, a high-concentration phosphate buffer solution, pH adjustment solution A, and pH adjustment solution B. The high-concentration extraction reagent is perchloric acid; the standard curve solution is a mixed solution of the nine flavor nucleotides; the high-concentration phosphate buffer solution is a solution of potassium dihydrogen phosphate and dipotassium hydrogen phosphate; pH adjustment solution A is sodium hydroxide; and pH adjustment solution B is phosphate. This invention also provides a method for the simultaneous detection of the nine flavor nucleotides using the above kit. This invention enables the simultaneous determination of the content of nine flavor nucleotides in livestock and poultry products. The method is simple, rapid, accurate, and sensitive, and can be used for batch determination, showing good prospects for practical application.

The present application relates to a kind of preparation method of full quality microencapsulation of fermentation liquor of gamma-aminobutyric acid produced by recombinant bacillus subtilis. Recombinant bacillus subtilis (strain preservation in China General Microbiological Culture Collection Center, strain preservation number is CGMCC No.5550) is used as starting strain to carry out fermentation. For recombinant bacillus subtilis fermentation liquor system, ethyl cellulose, glycerol, sucrose, yeast extract, dipotassium hydrogen phosphate and gelatin are selected to carry out microencapsulation preparation, spray drying. The optimal addition concentration (mass / volume) of capsule material in catalytic mixed solution is 2.5% ethyl cellulose, 1.5% glycerol, 2.5% sucrose, 2% yeast extract, 0.15% dipotassium hydrogen phosphate and 0.3% gelatin. The optimal rotation speed when capsule material is mixed with catalytic liquid is 60rpm, and the optimal stirring time is 30min;The optimal speed of high-speed dispersion is 15000rpm, and the optimal dispersion time is 3min. The optimal air inlet temperature of capsule material and catalytic liquid mixed dispersion liquid spray drying is 160℃, the optimal air inlet temperature is 70℃, and the optimal feeding rate is 1.5L / min.

The present application belongs to the technical field of biological fermentation, and relates to a spirodelated sophora ferments, a preparation method thereof and application of the spirodelated sophora ferments in preparation of anti-wrinkle firming and soothing care products. The spirodelated sophora, glucose, dipotassium hydrogen phosphate and water are mixed, and after enzyme inactivation and sterilization, a fermentation culture medium is prepared; Lactobacillus plantarum and Lactobacillus rhamnosus are mixed and inoculated into the fermentation culture medium, and then fermentation is carried out in an anaerobic environment. The present application utilizes two strains to ferment spirodelated sophora, which not only has mild conditions, can avoid high-temperature damage to active ingredients such as total phenol, polysaccharide, flavone and amino acid, avoids the addition of organic solvents, and significantly improves the content of active ingredients such as total phenol, polysaccharide, flavone and amino acid, which is conducive to industrial production. At the same time, the fermentation liquor has the effects of anti-wrinkle firming, antioxidant, soothing and care.

The present application relates to the technical field of fermentation medium, more specifically, it relates to a high-density fermentation medium for streptococcus suis and preparation and application thereof.The medium comprises beef infusion powder 1-5 parts, tryptone 1-10 parts, pancreatic casein peptone 1-10 parts, soybean peptone 1-5 parts, dipotassium hydrogen phosphate 1-5 parts, sodium chloride 1-10 parts, yeast extract 1-10 parts and glucose 1-5 parts.

The application discloses a method for controlling and inhibiting sugarcane acidification by using nitrogen-fixing bacteria, and relates to the technical field of controlling and inhibiting sugarcane acidification, and solves the problems that a combined nitrogen-fixing system formed by nitrogen-fixing bacteria and sugarcane is greatly affected by rhizosphere biological adversity, nitrogen-fixing effect is unstable, and nitrogen-fixing efficiency is low. The application reduces the use of nitrogen fertilizer in the whole growth cycle of the sugarcane, and applies a combined nitrogen-fixing bacterial agent and a promoting agent in the sugarcane seedling stage and the elongation stage, respectively. The combined nitrogen-fixing bacterial agent is Raoultella L03, Bacillus azoreus and Rhizosphaera sulcata, and the promoting agent is mannitol, glycerol, succinic acid, sodium molybdate, dipotassium hydrogen phosphate, magnesium sulfate, ferrous sulfate and glutathione. The application can make the nitrogen-fixing bacteria quickly form a combined nitrogen-fixing system with the sugarcane, so that the purposes of efficient nitrogen fixation, less nitrogen fertilizer application and soil acidification reduction are achieved.

The application discloses a kind of black Kui mushroom polysaccharide extract and preparation method thereof.The method uses microemulsion low eutectic solvent extraction formula to pretreat fungus powder, the formula is composed of homogeneous gel system of choline chloride, lactic acid, 1,3 butanediol, sucrose fatty acid ester, ethanol and complex enzyme, realizes the synergy of wall breaking and enzymolysis.Synchronous reaction is completed by gradient variable temperature treatment, and the extract is centrifuged by horizontal screw, and then double-water phase extraction purification is carried out using salting-out complexation synergistic precipitant containing dipotassium hydrogen phosphate, chitosan oligosaccharide, isopropyl alcohol and polyethylene glycol 200.The application simplifies the traditional six-step process to four steps, the extraction time is shortened to less than 90 minutes, the total sugar yield is more than 18%, the product purity is more than 90%, the complex enzyme dosage is reduced by 50%, the comprehensive cost is reduced by 22% compared with water extraction method, and the activity retention rate is increased to more than 95%, suitable for industrialized continuous production.

This invention discloses a pesticide synergist and its preparation method. The synergist is composed of 7-9 parts potassium oleate, 1-2 parts dipotassium hydrogen phosphate, and 1-2 parts 2-hydroxy-4-methylvaleric acid in a specific mass ratio. The preparation method involves stirring and mixing the above components at room temperature until completely dissolved to form a homogeneous solution. This synergist can be used in combination with chemical pesticides such as triadimefon, carbendazim, and pyraclostrobin. By enhancing the adhesion, penetration, and binding efficiency of pesticides to plant surfaces and pathogen targets, it can maintain over 90% control efficacy even with a 50%-80% reduction in chemical pesticide usage, significantly reducing pesticide residues and environmental risks. The raw materials are readily available, the process is simple, and the cost is low, making it suitable for large-scale production and of great significance for achieving reduced pesticide use and increased efficiency in agriculture and sustainable development.

This invention discloses a pesticide reduction and enhancement adjuvant and its preparation method, belonging to the field of pesticide technology. The adjuvant is composed of 7-9 parts potassium oleate, 1-2 parts dipotassium hydrogen phosphate, and 1-2 parts 2-ethylidene-5-phenylvaleric acid. The preparation method involves stirring and mixing the components at room temperature to form a homogeneous solution. When used in combination with chemical pesticides such as triadimefon, carbendazim, and pyraclostrobin, this adjuvant can significantly enhance pesticide efficacy. Even with a 50%-80% reduction in chemical pesticide usage, the control efficacy remains above 90%, effectively reducing pesticide residues and environmental risks. This invention has the advantages of simple composition, low cost, and environmentally friendly process, and is suitable for reducing pesticide use and increasing efficiency in agricultural production.

This invention provides a brain tissue equivalent material for medical imaging equipment and its preparation method, belonging to the technical field of tissue equivalent materials. The brain tissue equivalent material of this invention comprises the following raw materials in the indicated mass fractions: 20-35% bisphenol A epoxy resin, 10-15% octyl glycidyl ether, 1-5% urea, 1-5% calcium carbonate, 1-5% sodium dihydrogen phosphate, 1-5% dipotassium hydrogen phosphate, 15-35% polyethylene, 15-25% methyl methacrylate, and 5-15% trimethylhexanediamine. The CT value of the brain tissue equivalent material of this invention is highly consistent with that of real brain tissue, successfully covering the typical range of gray and white matter. The brain tissue equivalent material exhibits excellent wide-spectrum attenuation characteristics; within the commonly used energy range of 60-200 keV in clinical CT equipment, the deviation of the X-ray attenuation coefficient from the target brain tissue material is controlled within 5%, demonstrating excellent spectral stability and simulation accuracy.

This application belongs to the technical field of tire tread materials, specifically providing a tire tread material, its preparation method, and a tire. The tire tread material comprises a continuous phase and a dispersed phase. The continuous phase is methyl vinyl silicone rubber, and the dispersed phase includes carbon black, composite fillers, and additives. The composite filler is prepared from nano-silica, calcium-based nanoparticles, oleylamine, polysorbate, and thioctic acid carboxylic betaine. The calcium-based nanoparticles are prepared from calcium chloride, dipotassium hydrogen phosphate, potassium sulfate, and phenylboronic acid derivatives. The additives include reinforcing agents, accelerators, protective waxes, antioxidants, and stearic acid. The tire tread material prepared by this application has the advantages of good mechanical properties and low rolling resistance.

The present application relates to a kind of production of Fermentation Medium of trichotoxin and a kind of fermentation method for improving the yield of trichotoxin, belong to biological fermentation technical field.The present application uses pea starch, glucose as carbon source, uses soybean meal, corn serum as nitrogen source, with suitable inorganic salt (sodium chloride, dipotassium hydrogen phosphate, potassium dihydrogen phosphate, ammonium sulfate, magnesium sulfate, sodium selenite), metabolic auxiliary factor (pyridoxine) and amino acid, prepare a kind of suitable for bacillus subtilis production trichotoxin Fermentation Medium, the cost of the Fermentation Medium is low, and compared with the Fermentation Medium for producing trichotoxin in prior art, the yield of trichotoxin is significantly improved, provides new train of thought for the cost reduction and benefit increase in the process of large-scale production of trichotoxin.