180 results about "Column temperature" patented technology

The invention discloses a method for determining the purity of electronic-grade hexafluoroethane, and relates to the technical field of the purity of hexafluoroethane, and the method comprises the following steps: vacuumizing a specific sampling container to 10 <-3 > Pa to 10 <-2 > Pa, forming a super-hydrophobic coating on the inner wall, sampling in a temperature-controlled, humidity-controlled and pressure-controlled environment, and removing water and impurities through two-stage pretreatment; enhancing analysis by using a gas chromatograph, a specific carrier gas, a column temperature program, a micro-fluidic chip and an ion mobility spectrometry; then secondary scanning analysis is carried out by using a mass spectrometer double ion source and a calibration system; and finally, learning and matching an algorithm through a data processing system machine. Samples can be accurately collected and pretreated, gas chromatography and mass spectrometry analysis is more accurate and efficient, a data processing system can accurately determine impurities and purity, multiple measurement data can be counted and analyzed, the result reliability is improved, all parts of the system are optimized and matched, the accuracy and stability of the whole process are guaranteed, and the purity is calculated; the method further comprises statistical analysis of multiple measurement data.

The invention discloses a method for detecting a chiral isomer of 6-hydroxy-8-chloro ethyl caprylate. The method comprises the following steps: carrying out gas chromatography detection on a test solution containing R-6-hydroxy-8-chloro ethyl caprylate and S-6-hydroxy-8-chloro ethyl caprylate, wherein the gas chromatography detection is carried out on the test solution containing R-6-hydroxy-8-chloro ethyl caprylate and S-6-hydroxy-8-chloro ethyl caprylate; the conditions of the gas chromatography detection are as follows: a chromatographic column is selected from a chiral chromatographic column containing beta-cyclodextrin; the chromatographic column adopts programmed heating in the detection process, the initial column temperature is 120-180 DEG C, the final column temperature is 190-220 DEG C, and the heating rate is 0.5-2.0 DEG C / min. The method is based on a gas chromatographic method, adopts a beta-cyclodextrin-containing chiral chromatographic column, and is combined with programmed heating at a specific initial column temperature heating rate, so that the two chiral isomers of 6-hydroxy-8-chloro ethyl caprylate can be separated and detected, the sensitivity is high, the specificity is good, and the separation degree meets the standard; the method has important significance on preparation of single-configuration lipoic acid.

The invention relates to a chromatographic instrument temperature control method and system, an intelligent terminal and a storage medium, and relates to the technical field of chromatographic instrument sample separation.The method comprises the steps that the sample type of a preset sample is obtained; analyzing the sample type to determine a window heating rate and a window target temperature; acquiring a chromatographic column initial temperature of a preset chromatographic column; analyzing the initial temperature of the chromatographic column and the target temperature of the window to determine the temperature of the chromatographic column needing to be increased; analyzing the temperature required to be increased and the window heating rate of the chromatographic column so as to determine the heating time of the chromatographic column; and controlling a preset chromatographic column temperature regulation and control device to perform temperature control on the chromatographic column according to the chromatographic column heating time and the window target temperature. The method has the effect of improving the precision of the temperature retention time.

The invention relates to the technical field of liquid chromatographs, and discloses a liquid chromatograph for ademetionine detection, which comprises a tray, a controller, an on-line degasser, a first pump machine, a second pump machine, a detector, an automatic sampler I, an automatic sampler II and a column oven, and is characterized in that the on-line degasser is arranged on the tray; the tray, the controller, the online degasser, the first pump and the second pump are sequentially distributed from top to bottom, the detector, the first automatic sampler and the second automatic sampler are sequentially distributed from top to bottom, the second pump, the second automatic sampler and the column oven are sequentially distributed from left to right, and four solvent bottles are placed in the tray. The solvent bottle is stabilized by arranging the fixing assembly, the suction filtration assembly adopts a replaceable filter element structure to avoid blockage, a T-shaped block and a tension spring are designed below the sample rack to ensure resetting, a cleaning assembly and an exhaust fan are arranged in the column oven to quickly clean leaked liquid and accelerate drying, and the stability, the maintenance convenience and the working efficiency of the equipment are improved.

According to the method, a polysaccharide derivative bonding type chiral chromatographic column with silica gel surface bonded amylose-tri (3, 5-dichlorophenyl carbamate) as a filler is adopted, a mixed solution of n-hexane and isopropanol is used as a mobile phase, the column temperature is 28-32 DEG C, the flow velocity is 1.1-1.3 ml / min, the column temperature is 20-30 DEG C, the column temperature is 20-30 DEG C, the column temperature is 20-30 DEG C, the column temperature is 20-30 DEG C, the column temperature is 20-30 DEG C, and the column temperature is 20-30 DEG C; an ultraviolet detector is adopted, the wavelength is 205-220 nm, and effective separation of the etomidate intermediate and the enantiomer thereof is achieved. The method is good in detection result accuracy and high in sensitivity, and compared with an existing analysis method, the method is better in separation effect, simpler in mobile phase composition, more durable in chromatographic column and lower in cost, so that a good guarantee is provided for quality control of the etomidate intermediate and etomidate.

The invention belongs to the field of ion chromatography, and particularly relates to a temperature adjusting method and device for an ion chromatograph column oven, and the method comprises the steps: firstly, building a temperature change model based on the Newton's cooling law; after a heat source in the column oven begins to heat, three time points t1, t2 and t3 are selected, t3-t2 is made to be equal to t2-t1, cavity temperatures T1, T2 and T3 corresponding to temperature sensors in the column oven at the three moments are recorded, and temperature models corresponding to the moments are built; logarithms of the model formulas of the cavity temperature values T1, T2 and T3 at the three moments t1, t2 and t3 are taken and calculated to obtain a prediction model of the final cavity temperature value T; establishing an equilibrium state equation, and directly adjusting the heat source according to the calculated heat source temperature. According to the method, the waiting time after adjustment is shortened, and the time required by a temperature control system is shortened; and the temperature required to be set by the heating body can be directly obtained, and the operation can be completed through one-time adjustment without repeated adjustment, so that the energy consumption is reduced, and the working efficiency is improved.

The invention discloses an independent liquid chromatography column oven, and relates to the technical field of column ovens, the independent liquid chromatography column oven comprises a column oven body, the inner side of the column oven body is fixedly connected with a plurality of separation frames, the inner sides of the separation frames are fixedly connected with supporting blocks, the outer sides of the supporting blocks are provided with liquid chromatography columns, and the liquid chromatography columns are arranged on the outer sides of the liquid chromatography columns. Connectors are fixedly connected to the left end and the right end of the liquid chromatographic column, a mounting unit for mounting the liquid chromatographic column is arranged on the inner side of the separation frame, and a fixed cover and a pump body are fixedly connected to the left side of the column oven body. According to the column oven for the independent liquid chromatography, a plurality of separation frames are arranged, a plurality of chromatographic columns are contained, the same instrument and multi-item convenient switching detection are facilitated, and through a guide plate, a guide block, a fixing rod, an installation box, a rotating pipe, installation heads, a second gear piece, a toothed plate and an electric push rod, the installation heads on the left side and the right side are conveniently controlled to rotate and get close to each other; and the liquid chromatographic column is quickly connected with the pump body, so that the workload of workers is reduced, and the operation is simple.

The invention belongs to the field of ion chromatography, and particularly relates to a temperature prediction method and device for an ion chromatograph column oven, and the method comprises the steps: firstly constructing a temperature change model based on the Newton's cooling law; after a heat source in the column oven begins to heat, three time points t1, t2 and t3 are selected, t3-t2 is made to be equal to t2-t1, cavity temperatures T1, T2 and T3 corresponding to temperature sensors in the column oven at the three moments are recorded, and temperature models corresponding to the moments are built; logarithms of the model formulas of the cavity temperature values T1, T2 and T3 at the three moments t1, t2 and t3 are taken and calculated to obtain a prediction model of the final cavity temperature value T; and calculating and comparing the final temperature value T final value of the cavity with the required temperature value T required of the cavity, and adjusting the temperature T heat source of the heat source in advance. According to the method, the waiting time after each adjustment is reduced, the time required by the temperature control system is shortened, the response speed of the system is improved, the energy consumption can be reduced, and the working efficiency is improved.

The invention relates to the technical field of food safety detection, in particular to a method for detecting urea in bean sprouts and application thereof.The method comprises the following steps that smashed bean sprouts are subjected to two times of ultrasonic extraction and centrifugation, supernate is combined, and an extracting solution is obtained; fixing the volume and filtering to obtain a to-be-detected solution; detecting the liquid to be detected by using liquid chromatography-tandem mass spectrometry; an extraction solvent for ultrasonic extraction is a 5% formic acid-acetonitrile composite solution; the conditions of the liquid chromatography-tandem mass spectrometry are as follows: the chromatographic conditions are as follows: a chromatographic column is an HILIC column; the sample size is 10 [mu] L; a mobile phase is a mixed solution of a 0.02 mmol / L ammonium acetate buffer salt solution and acetonitrile, and isocratic elution is carried out; the flow rate is 0.3 mL / min; the column temperature is 40 DEG C; mass spectrum conditions are as follows: an electrospray ionization (ESI) positive ion mode, electrospray voltage of 4000 V, sheath gas pressure of 30 psi, auxiliary gas pressure of 5 psi, capillary heating temperature of 300 DEG C and scanning mode of MRM. The method disclosed by the invention is free from substance interference, high in sensitivity, accurate in result, low in cost and high in efficiency, and can be used as a standard detection method.

The invention discloses a method for analyzing related substances and impurities in a reaction process of Mabazavir, relates to the field of medicine detection, and aims to provide a high-sensitivity and excellent-stability related substance analysis method for controlling the reaction process of Mabazavir, and the chromatographic conditions are as follows: a C18 chromatographic column (Waters Xselect CSH C18, 4.6 * 100mm, 3.5 mu m) of which the surface is charged and hybridized is adopted as a chromatographic column; a mobile phase A is a 0.05%-0.2% trifluoroacetic acid solution containing 0.1-1.0 mmol / L disodium ethylene diamine tetraacetate, a mobile phase B is acetonitrile, and isocratic elution is carried out according to the ratio of 55: 45; the column temperature is 40-60 DEG C; the flow velocity is 0.5 to 1.0 mL / min; the sample size is 5-20 [mu] l; and the operation time is 20-60 minutes. The method has the advantages of high detection sensitivity, good reproducibility, high stability and the like, and is suitable for detecting related substances controlled in the reaction process of the macavir.

The invention discloses a method for simultaneously detecting 37 phenolic substances in a plant based on HPLC (High Performance Liquid Chromatography). HPLC-PDA (Personal Digital Assistant) is adopted for separation and detection; the detection conditions are as follows: the chromatographic column is a PFP chromatographic column; the detection wavelengths are 280 nm, 330 nm and 360 nm; the flow velocity is 0.5 to 0.8 mL / min; the sample size is 8-12 [mu] L; the column temperature is 28-32 DEG C; the mobile phase A is an aqueous solution containing 0.3% by mass of formic acid, and the mobile phase B is methanol; the elution gradient is 0-10 min, and the elution gradient is 5-10% B; in 10-16 min, 10%-25% of B is obtained; after 16 to 25 minutes, 25 to 40 percent of B is obtained; 25 to 50 minutes, 40 to 60 percent of B is adopted; and 60-67% of B is carried out in 50-55 min. The method is suitable for plant sample detection, is especially suitable for sea-buckthorn sample detection, can be applied to multiple fields of food analysis, plant research, component development and the like, and has wide application value.

The invention discloses a method for determining dextrocarnitine in a levocarnitine product by using high performance liquid chromatography, and the chromatographic conditions are as follows: cellulose-tri-(3, 5-dimethyl phenyl carbamate) is used as a filler for a chromatographic column, a hexafluorophosphate buffer solution-acetonitrile is used as a mobile phase, the volume ratio is 77: 23-69: 31, the column temperature is 15-25 DEG C, the flow velocity is 0.3-0.5 ml / min, and the detection wavelength is 246nm. The method disclosed by the invention can be used for rapidly and accurately detecting the dextrocarnitine in the levocarnitine product and calculating the content of the dextrocarnitine, is simple to operate and good in reproducibility, can better realize quality control of the levocarnitine preparation and an intermediate product thereof, and provides guarantee for synthesis and preparation process optimization.

The invention relates to the technical field of chemical detection, and particularly discloses a method for detecting impurities in propafenone hydrochloride injection. The invention discloses a method for detecting impurities in propafenone hydrochloride injection, which comprises the following steps: detecting a sample to be detected by using high performance liquid chromatography to obtain the content of the impurities, in the detection process, octadecylsilane chemically bonded silica is used as a filling agent of a chromatographic column; the column temperature is 28-32 DEG C, and the flow velocity is 0.8-1.2 ml / min; the mobile phase comprises a mobile phase A and a mobile phase B, the mobile phase A is 0.012-0.017 mol / L dipotassium phosphate solution, and the pH value of the mobile phase A is adjusted to 2.2-2.8 with phosphoric acid; and the mobile phase B is acetonitrile. The detection method provided by the invention is simple to operate and high in detection speed, and has the advantages of high sensitivity, high precision, high repeatability and high accuracy.

The invention belongs to the technical field of separation and detection, and particularly relates to a method for determining iodide in domestic drinking water. Measuring the iodide in the domestic drinking water by adopting a high performance liquid chromatography-ultraviolet detection method; taking a domestic drinking water sample solution for detection, and quantifying by an external standard method; according to the detection method, a Metrosep A Supp5-250 chromatographic column (250 mm * 4.0 mm, 5 [mu] m) is adopted for separation, 4.5 mmol / L Na2CO3, 1.0 mmol / L NaHCO3 and 35% acetonitrile are adopted as a mobile phase, a DAD detector is adopted, the wavelength is 226 nm, the flow rate is 0.60 mL / min, and the column temperature is 50 DEG C. The invention also discloses a method for detecting the content of the sodium hydroxide in the sample. According to methodology verification, the method can be used for measuring iodide in domestic drinking water, the detection limit is 2.0 mu g / L and is superior to that of a spectrophotometric method and a volumetric method in the national standard, no complex pretreatment process exists, specificity is high, toxic and harmful chemical reagents are not used, online automatic sample injection can be achieved, and the method has obvious advantages for large-batch samples; compared with inductively coupled plasma mass spectrometry, used instruments are relatively cheap, and basic popularization and application are facilitated.

The invention discloses a method for simultaneously detecting various sugars and sugar alcohols. The method comprises the following steps: 1) preparing a sample solution to be detected; (2) carrying out qualitative or quantitative detection on sugar and sugar alcohol in the sample solution to be detected by adopting an ultra-high performance liquid chromatography-mass spectrometry method; wherein the chromatographic separation conditions of the ultra-high performance liquid chromatography-mass spectrometry method are as follows: the chromatographic column is Waters Acquity BEH Amide 2.1 * 100 mm, the column temperature is 20-30 DEG C, and the column temperature is 20-30 DEG C; the column temperature is 10 DEG C; the flow velocity is 0.2 to 0.5 mL / min; the sample size is 0.1 to 1 [mu] L; mobile phases are as follows: a phase A is 0.1% formic acid water, a phase B is acetonitrile, and gradient elution is performed. The method can be used for simultaneously detecting ten sugars and sugar alcohols, can also be used for simultaneously detecting isomers of various sugars, and has very high sensitivity.

The invention relates to the technical field of chemical analysis, and particularly discloses a method for detecting a residual solvent in 1, 3, 5-trihydroxybenzene. A chromatographic column with 6% cyanopropyl benzene-94% dimethyl siloxane as a stationary liquid is adopted as a detection chromatographic column, the column temperature is set to be 35-45 DEG C and maintained for 4-6 min, then the temperature is increased to 195-205 DEG C at the rate of 8-12 DEG C / min and maintained for 4-6 min, the temperature of a constant-temperature furnace is 95-105 DEG C, the temperature of a detector is 255-265 DEG C, the split ratio is 8-12: 1, and the detection of 1, 3, 4-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5 The method can be used for simultaneously and quantitatively detecting the residual solvent 1, 3, 5-trihydroxybenzene, namely the 1, 3, 5-trihydroxybenzene, namely the 1, 3, 5-trihydroxybenzene, namely the 1, 3, 5-trihydroxybenzene, the methyl alcohol and the ethyl acetate, is more beneficial to reflecting the quality of the 1, 3, 5-trihydroxybenzene, and is of great significance for establishing a quality control system of the 1, 3, 5-trihydroxybenzene and ensuring the product quality and the medication safety of the 1, 3, 5-trihydroxybenzene.

This utility model relates to a column housing device for housing a chromatographic column within a column oven. The device includes: a housing with a base having a first side and an opposite second side, wherein the first side has a groove; a first sidewall and a second sidewall extending from the first side of the base, wherein the second sidewall is arranged opposite to the first sidewall; and a support member including a bracket disposed in the groove, such that the support member is detachably fixed to the first side of the base via the bracket between the first and second sidewalls, thereby forming a heat exchange channel between the base, the support member, and the first and / or second sidewalls for placing the chromatographic column. This column housing device detachably connects the support member to the housing, providing a flexible and adjustable thermal structure that balances column placement stability and heat transfer efficiency. This utility model also relates to a column oven and a chromatogram.

The invention provides a system and method for detecting the content of a corrosion inhibition additive in cooling liquid, specifically, the system comprises a liquid chromatograph, a diode array detector DAD and an electric fog detector CAD which are sequentially connected in series, the liquid chromatograph comprises a mobile phase pump, an automatic sampler and a column oven, and a chromatographic column is arranged in the column oven; wherein the mobile phase pump is used for mixing different mobile phases according to a preset proportion and then conveying the mixed mobile phases to the automatic sample injector; the automatic sample injector is used for injecting the mobile phase from the mobile phase pump into the to-be-detected cooling liquid and flushing the to-be-detected cooling liquid injected with the mobile phase to the chromatographic column; the column oven is used for controlling the column temperature of the chromatographic column; the chromatographic column is used for performing chromatographic separation on the corrosion inhibition additive in the to-be-detected cooling liquid; the DAD is used for detecting the corrosion inhibition additive containing the benzene ring in the cooling liquid to be detected; the CAD is used for detecting the polybasic aliphatic carboxylic acid type corrosion inhibition additive in the cooling liquid to be detected.

The application relates to the technical field of data processing, and provides a method for calculating a temperature field, which comprises the following steps: determining a first density of first column temperature sampling points; determining the number A of temperature sampling points between a first temperature sensor and a second temperature sensor according to a first interval between the first temperature sensor and the second temperature sensor and the first density; determining A temperature values through interpolation operation according to temperature values of the first temperature sensor and the second temperature sensor; determining a second density of first row temperature sampling points; determining the number B of temperature sampling points between the first temperature sampling points and the second temperature sampling points according to a second interval between the first temperature sampling points and the second temperature sampling points and the second density; determining B temperature values through interpolation operation according to temperature values of the first temperature sampling points and the second temperature sampling points; and determining the temperature field of a machine room according to the A temperature values and the B temperature values. The method can improve the efficiency of calculating the temperature field.

The invention relates to a method for analyzing and detecting a non-azonetin enantiomer, which adopts normal-phase high performance liquid chromatography and takes normal hexane-ethanol-trifluoroacetic acid as a mobile phase to detect the non-azonetin enantiomer, and the specific chromatographic conditions are as follows: a chromatographic column is prepared by covalently bonding amylose-tri (3, 4, 6-trimethyl-1, 3-pentanediol) on the surface of silica gel; 2, 5-dichlorophenyl carbamate is used as a filling agent (IE, 4.6 * 250 mm, 5.0 [mu] m); a mobile phase: n-hexane: ethanol: trifluoroacetic acid = 500: 500: 1 (V / V / V); the flow rate is 1.0 ml / min; the column temperature is 30 DEG C; the sample size is 20 [mu] l; the detection wavelength is 280 nm; the elution gradient is isocratic elution. The invention provides a high performance liquid chromatography method for analyzing the enantiomer of the fiazonetin, the complete separation of the fiazonetin and the enantiomer of the fiazonetin can be realized by adopting the method, and the separation degree of a main component and the enantiomer is greater than 1.5.

The invention relates to the technical field of biological analysis, in particular to a fish and crustacean biological analysis device which comprises a placing frame and a mobile phase solvent bottle placed in the upper portion of the placing frame, a vacuum degassing box is connected to the lower portion of the placing frame, and a high-pressure pump box is connected to the lower portion of the vacuum degassing box. A sample injection box is connected to the lower part of the high-pressure pump box, a detection box is connected to the lower part of the column oven, an automatic sample injector is arranged in the sample injection box above the placing frame, and a chromatographic column is connected to the interior of the column oven. According to the biological analysis device for the fishes and the crustaceans, through slight shaking in multiple directions, the uniform shaking efficiency and effect of a sample solution in the sample solvent bottle can be improved, the situation that particles settle to the bottom of the bottle and are layered due to long-time standing of the sample solvent bottle is avoided, the concentration of the sample solution sucked by an automatic sample injector is uniform, and the accuracy of the sample solution is improved. Therefore, the accuracy of the detection result of the biological analysis device can be improved, and the biological analysis work of fishes and crustaceans cannot be influenced.

The utility model discloses a column temperature box with automatic chromatographic column selection system, including the box, the box surface is equipped with the door body of rotation connection, is equipped with the valve cut system in the box to the door body one side, and the valve cut system includes the mounting panel in the box top, the chromatographic column installation valve head on the mounting panel, the control valve in the chromatographic column installation valve head tail portion, is equipped with the temperature control area below the valve cut system, and the temperature control baffle that installs in the box is equipped in the temperature control area, and the mounting bracket on the surface of temperature control baffle and the chromatographic column on the mounting bracket, the bottom of temperature control area still is equipped with the liquid leakage device.

The invention discloses a method for detecting 5 '(E)-VPA phosphoramidite monomer related substances, and belongs to the technical field of liquid chromatography detection. Related substances comprise 5 '-(E)-VP-2'-OMe-A (Bz), 5 '(Z)-VPA, 5'-(E)-VP-2 '-OMe-A-CE-P and 5' (E)-VPA-OX, and chromatographic detection conditions comprise that the column temperature is 23-27 DEG C, a stationary phase of a chromatographic column is octadecyl bonded silica gel, a mobile phase A is an ammonium acetate solution, a mobile phase B is acetonitrile, gradient elution is performed, and the like. According to the method, four related substances in the 5 '(E)-VPA phosphoramidite monomer are effectively separated through specific liquid chromatography conditions, the system adaptability, specificity, linearity and range, detection limit, quantitation limit and accuracy verification of the method all meet acceptable standards of pharmacopoeia, and detection of multiple related substances can be completed within a short time; and a reliable means is provided for detecting and controlling the quality of the 5 '(E)-VPA phosphoramidite monomer.

< TableDetails number = '0001' > < Title / > < tbl: tgroup cool = '3' > < tbl: colspec cool = 'c001' cool width = '35%' / > < tbl: colspec cool width = 'c002' cool width = '32%' / > < tbl: tbody > < tbl: start = '1' > time < / The column temperature is 60-70 DEG C; the temperature of the sample chamber is 2-8 DEG C; and (3) detecting the sample to be detected. According to the method, the P188 is effectively separated, and the P188 is accurately and quantitatively analyzed.

The invention belongs to the technical field of analysis and testing, and particularly relates to a method for measuring the optical purity of L-lactide. The method comprises the following steps: taking a CHIRALP AK-H chiral column as a chromatographic column; n-hexane-isopropanol is used as a mobile phase; the column temperature is 25-35 DEG C; the detection wavelength is 208 to 212nm; the method provided by the invention has the advantages that the sample introduction volume is 20 [mu] l, effective separation and quantitative detection of meso-lactide, L-lactide and D-lactide are realized, the detection result is accurate and reliable, and a basis is provided for quality control of L-lactide.

The invention belongs to the technical field of gas chromatography detection, and relates to a gas chromatography method for simultaneously determining the content of ethanol and isopropyl myristate. The set conditions of the gas chromatography are as follows: the temperature of an FID detector and a polyethylene glycol 20M capillary column is 240-260 DEG C, the temperature of the detector is 240-260 DEG C, the carrier gas is nitrogen, the flow rate is 0.9-2.5 mL / min, the sample injection amount is 1 mu L, the column temperature is increased by adopting a program, the initial temperature is 80-90 DEG C, the temperature is increased to 180-190 DEG C at the rate of 9-12 DEG C / min, and the temperature is maintained for at least 10 minutes. The method provided by the invention can realize simultaneous detection of ethanol and isopropyl myristate, after the two substances are dissolved by the N, N-dimethylformamide solvent, the detected spectrogram does not have the phenomena of peak overlapping, trailing and the like, and the method has the advantages of high separation efficiency, high selectivity, high sensitivity, high analysis speed and wide application range.

The invention discloses a method for determining the content of poloxamer 124 in the technical field of medical analysis. Liquid chromatography is adopted for detection, and a gel chromatographic column filled with porous silicon dioxide is selected as a chromatographic column; a mobile phase is a trifluoroacetic acid-acetonitrile-water solution; the weight ratio of the mobile phase trifluoroacetic acid to acetonitrile to water is 0.1: (18-22): (78-82); the column temperature is 25-45 DEG C; the temperature of a detector is 60-80 DEG C; the flow velocity of the mobile phase is 0.6-1.0 ml / min; the sample size is 5-20 [mu] l, automatically injecting the sample according to the chromatographic conditions, recording the peak area, and calculating the content according to an area normalization method. The method is high in response value, simple in detection operation and high in product content accuracy, specificity, repeatability, sampling precision, linearity and range all meet the requirements, and the quantitative determination problem of poloxamer 124 is solved.

The present invention provides a method for simultaneously determining 5-hydroxymethylfurfural and its oxidation product, including: providing a reaction mixture obtained after 5-hydroxymethylfurfural is oxidized, the reaction mixture comprising 5-hydroxymethylfurfural and its oxidation product; filtering the reaction mixture to obtain a filtrate and a filter residue; preparing the filtrate as a first solution to be tested, mixing the filter residue with an inorganic base so that the insoluble oxidation product in the filter residue undergoes a salt-forming reaction and dissolves, and preparing the filtrate as a second solution to be tested; performing high performance liquid chromatography analysis on the first solution to be tested and the second solution to be tested, respectively, the conditions for high performance liquid chromatography analysis comprising: an organic acid analytical column as the stationary phase, and a column temperature of 25°C to 60°C; an inorganic acid aqueous solution as the mobile phase, and a flow rate of the mobile phase of 0.4 to 1 mL / min; an injection volume of 1 μL to 20 μL; and adopting dual wavelength detection, with a first detection wavelength of 200 nm to 240 nm and a second detection wavelength of 250 nm to 300 nm. The method provided by the present invention has good separation, high accuracy, and high precision.

The present invention discloses a stand-alone column temperature box for liquid chromatography, which relates to the technical field of column temperature boxes and includes a column temperature box body, wherein a plurality of partition frames are fixedly connected to the inner side of the column temperature box body, a support block is fixedly connected to the inner side of the partition frame, a liquid chromatography column is arranged on the outer side of the support block, the left and right ends of the liquid chromatography column are fixedly connected with connectors, the inner side of the partition frame is provided with an installation unit for installing the liquid chromatography column, and the left side of the column temperature box body is fixedly connected with a fixed cover and a pump body. The stand-alone column temperature box for liquid chromatography, by being provided with a plurality of partition frames, can accommodate a plurality of chromatography columns, so that the same instrument can conveniently switch and detect multiple projects, and through the guide plate, guide block, fixed rod, installation box, rotating tube, installation head, gear part 2, tooth plate and electric push rod, it is convenient to control the installation heads on the left and right sides to rotate and approach each other, so that the liquid chromatography column can be quickly connected to the pump body, reducing the workload of the staff and simplifying the operation.

The invention discloses a method for determining ceftizoxime sodium for injection and impurities thereof by liquid chromatography, which comprises the following steps: taking octadecylsilane chemically bonded silica as a filler for the ceftizoxime sodium for injection; the column temperature is 35 DEG C; the flow rate is 0.8 ml / min; the detection wavelength is 254 nm; the sample injection volume is 20 microliters; carrying out gradient elution by using a mobile phase A and a mobile phase B; according to the method, the retention time of the impurity 16 can be stabilized, the impurity 09 and the impurity 12 can be effectively separated, and the method has important significance on quality control of the ceftizoxime sodium for injection.