The invention relates to a schisandra fruit fingerprint spectrum quality control method, comprising that (1), adding 0.5g schisandra fruit to extract via microwave for 50min, filtering, (2), washing flow phase gradient that the spectrum column is Eclipse, as 0-40min, 60-20% A, 40-80% B, flow speed is 0.8-1.2ml/min, the wavelength is 210-280nm, the column temperature is 20-40Deg. C, and the sample amount is 10-20ul, (3), building standard fingerprint spectrum that the first peak is schisandra fruit alcohol 1, the third peak is schisandra fruit alcohol 2, the fourth peak is gemixin N/ isomer, the sixth peak is schisandra fruit I element, the seventh and eighth peaks are gemixin N/ isomer, the ninth peak is schisandra fruit II element, (4), controlling the quality of fingerprint spectrum that the check peak relative holding times are 1.00, 1.25, 1.63, 1.88, 3.18 and 3.27, (5), the schisandra fruit planting collecting method. The invention can control the quality of materials to assure the stable quality of product.