56 results about "Column temperature" patented technology

This utility model relates to a column housing device for housing a chromatographic column within a column oven. The device includes: a housing with a base having a first side and an opposite second side, wherein the first side has a groove; a first sidewall and a second sidewall extending from the first side of the base, wherein the second sidewall is arranged opposite to the first sidewall; and a support member including a bracket disposed in the groove, such that the support member is detachably fixed to the first side of the base via the bracket between the first and second sidewalls, thereby forming a heat exchange channel between the base, the support member, and the first and / or second sidewalls for placing the chromatographic column. This column housing device detachably connects the support member to the housing, providing a flexible and adjustable thermal structure that balances column placement stability and heat transfer efficiency. This utility model also relates to a column oven and a chromatogram.

The invention provides a system and method for detecting the content of a corrosion inhibition additive in cooling liquid, specifically, the system comprises a liquid chromatograph, a diode array detector DAD and an electric fog detector CAD which are sequentially connected in series, the liquid chromatograph comprises a mobile phase pump, an automatic sampler and a column oven, and a chromatographic column is arranged in the column oven; wherein the mobile phase pump is used for mixing different mobile phases according to a preset proportion and then conveying the mixed mobile phases to the automatic sample injector; the automatic sample injector is used for injecting the mobile phase from the mobile phase pump into the to-be-detected cooling liquid and flushing the to-be-detected cooling liquid injected with the mobile phase to the chromatographic column; the column oven is used for controlling the column temperature of the chromatographic column; the chromatographic column is used for performing chromatographic separation on the corrosion inhibition additive in the to-be-detected cooling liquid; the DAD is used for detecting the corrosion inhibition additive containing the benzene ring in the cooling liquid to be detected; the CAD is used for detecting the polybasic aliphatic carboxylic acid type corrosion inhibition additive in the cooling liquid to be detected.

The application relates to the technical field of data processing, and provides a method for calculating a temperature field, which comprises the following steps: determining a first density of first column temperature sampling points; determining the number A of temperature sampling points between a first temperature sensor and a second temperature sensor according to a first interval between the first temperature sensor and the second temperature sensor and the first density; determining A temperature values through interpolation operation according to temperature values of the first temperature sensor and the second temperature sensor; determining a second density of first row temperature sampling points; determining the number B of temperature sampling points between the first temperature sampling points and the second temperature sampling points according to a second interval between the first temperature sampling points and the second temperature sampling points and the second density; determining B temperature values through interpolation operation according to temperature values of the first temperature sampling points and the second temperature sampling points; and determining the temperature field of a machine room according to the A temperature values and the B temperature values. The method can improve the efficiency of calculating the temperature field.

The invention relates to the technical field of biological analysis, in particular to a fish and crustacean biological analysis device which comprises a placing frame and a mobile phase solvent bottle placed in the upper portion of the placing frame, a vacuum degassing box is connected to the lower portion of the placing frame, and a high-pressure pump box is connected to the lower portion of the vacuum degassing box. A sample injection box is connected to the lower part of the high-pressure pump box, a detection box is connected to the lower part of the column oven, an automatic sample injector is arranged in the sample injection box above the placing frame, and a chromatographic column is connected to the interior of the column oven. According to the biological analysis device for the fishes and the crustaceans, through slight shaking in multiple directions, the uniform shaking efficiency and effect of a sample solution in the sample solvent bottle can be improved, the situation that particles settle to the bottom of the bottle and are layered due to long-time standing of the sample solvent bottle is avoided, the concentration of the sample solution sucked by an automatic sample injector is uniform, and the accuracy of the sample solution is improved. Therefore, the accuracy of the detection result of the biological analysis device can be improved, and the biological analysis work of fishes and crustaceans cannot be influenced.

The utility model discloses a column temperature box with automatic chromatographic column selection system, including the box, the box surface is equipped with the door body of rotation connection, is equipped with the valve cut system in the box to the door body one side, and the valve cut system includes the mounting panel in the box top, the chromatographic column installation valve head on the mounting panel, the control valve in the chromatographic column installation valve head tail portion, is equipped with the temperature control area below the valve cut system, and the temperature control baffle that installs in the box is equipped in the temperature control area, and the mounting bracket on the surface of temperature control baffle and the chromatographic column on the mounting bracket, the bottom of temperature control area still is equipped with the liquid leakage device.

The invention belongs to the technical field of gas chromatography detection, and relates to a gas chromatography method for simultaneously determining the content of ethanol and isopropyl myristate. The set conditions of the gas chromatography are as follows: the temperature of an FID detector and a polyethylene glycol 20M capillary column is 240-260 DEG C, the temperature of the detector is 240-260 DEG C, the carrier gas is nitrogen, the flow rate is 0.9-2.5 mL / min, the sample injection amount is 1 mu L, the column temperature is increased by adopting a program, the initial temperature is 80-90 DEG C, the temperature is increased to 180-190 DEG C at the rate of 9-12 DEG C / min, and the temperature is maintained for at least 10 minutes. The method provided by the invention can realize simultaneous detection of ethanol and isopropyl myristate, after the two substances are dissolved by the N, N-dimethylformamide solvent, the detected spectrogram does not have the phenomena of peak overlapping, trailing and the like, and the method has the advantages of high separation efficiency, high selectivity, high sensitivity, high analysis speed and wide application range.

The invention provides a method for determining D-psicose in processed food and application of the method. The method specifically comprises the following steps: extracting a sample with warm water at 50-60 DEG C, precipitating protein by adopting a Carrez reagent, and removing interference impurities; chromatographic conditions are defined as follows: a calcium type strong cation exchange column is adopted, pure water containing 50 mg / L of EDTA-Ca is adopted as a mobile phase, the flow rate is 0.4-0.6 mL / min, and the column temperature is 80 DEG C. The result shows that the linear relation of the D-psicose is good in the concentration range of 1.0 mg / mL to 20.0 mg / mL, and the correlation coefficient is 0.999999; according to the method, the LOD is 0.002 g / 100g, and the LOQ is 0.005 g / 100g. Under the experimental conditions of standard addition before simulation processing, the average recovery rate of 18 matrixes including dairy products, bakery products and the like is 81.5-128.1%, and the RSD is less than 3.0%. The determination method provided by the invention is simple in pretreatment, high in sensitivity, strong in interference resistance and good in accuracy, effectively solves the ubiquitous problems of matrix wrapping, matrix interference, difficulty in isomer separation and the like when the D-psicose in the processed food is detected, can realize rapid and accurate detection of the D-psicose in all dosage forms of processed food, and has a wide prospect.

The invention discloses a method for improving the detection recovery rate of ascorbyl palmitate in a compound product, which comprises the following steps: taking a sample, adding BHT (butylated hydroxytoluene), then adding an isopropanol-n-hexane mixed solution containing 0.8-1.2% by volume of glacial acetic acid as an extraction solvent, then fully mixing, extracting, freezing, centrifuging, taking supernate, and collecting the supernate to obtain the ascorbyl palmitate. The chromatographic conditions are as follows: a C18 reversed-phase chromatographic column is adopted, a mobile phase A and a mobile phase B are used for gradient elution, the flow velocity of the mobile phase is 0.8-1.2 mL / min, the column temperature is 28-32 DEG C, and the maximum absorption wavelength of ascorbyl palmitate is taken as the detection wavelength. According to the method, the extraction efficiency is improved by optimizing a sample pretreatment process, and the problem that the liquid phase detection error of the substance in the compound product is generally higher than 10% is effectively solved in combination with improved high performance liquid chromatography separation conditions.

The invention relates to the technical field of polypeptide detection, in particular to a method for detecting the content of a semeglutide polymer. A liquid chromatography method is adopted, wherein a chromatographic column is G2000 SWxl; carrying out isocratic elution for 40 minutes; the detection wavelength is 280 nm; the column temperature is 26-30 DEG C; the mobile phase is formed by mixing a phosphate solution of sodium chloride and isopropanol according to the volume ratio of 50: 50. The selected mobile phase has no influence on the retention performance of the chromatographic column, the separation degree of the polymer and the main peak is greater than 1.5, and the requirement on an instrument is lower; accurate and reliable experimental results can be obtained by determining the polymer in the semeglutide through the method.

The invention belongs to the technical field of pharmaceutical analysis, and discloses a liquid chromatography detection method of a pimobendan intermediate, which comprises the following steps: injecting octadecyl bond and silica gel as a stationary phase, a phosphate buffer solution with the pH value of 3.0-5.0 as a water phase and methanol (or acetonitrile) as an organic phase into a high performance liquid chromatograph under the conditions that the column temperature is 30-60 DEG C, the sample injection is 5-30 mu L and the flow velocity is 0.5-2 ml / min, and carrying out gradient elution to obtain the pimobendan intermediate. And the sample is analyzed in a wavelength range of 200-230nm. By adjusting the mobile phase system and optimizing the mobile phase gradient, a main peak and an impurity peak are completely separated, the analysis speed is high, the detection sensitivity is high, and the stability and the quality of a product in production can be better detected.

The application discloses a method for improving the chromatographic separation degree of impurities A and B in acetylcysteine raw materials, and is characterized by the following steps: controlling the column temperature of a chromatographic column within a specific low temperature range of 15 DEG C plus or minus 2 DEG C, and using the difference between the distribution behaviors of impurities A and B between a stationary phase and a mobile phase at different temperatures to significantly improve the separation degree of the two impurities. When the column temperature is 15 DEG C, the separation degree of impurities A and B can reach 2.06, which is much higher than the pharmacopoeia standard, and the separation degrees under the conditions of 30 DEG C, 35 DEG C and 40 DEG C all fail to meet the standard. The application does not need to replace the chromatographic column, is simple and convenient to operate, low in cost, can stably meet the accurate quantification requirements of related substances in acetylcysteine raw materials, and has good popularization and application value.

The invention relates to the technical field of analysis and detection, in particular to a gas chromatography-mass spectrometry detection method for N-methylaniline. The gas chromatography-mass spectrometry detection method for N-methylaniline comprises the following steps: treating a sample to be neutral, and extracting N-methylaniline in the sample by using dichloromethane to obtain a liquid to be detected; aa, separating the to-be-detected liquid through a quartz capillary column, and carrying out gas chromatography-mass spectrometry detection; the conditions of the gas chromatography are as follows: the temperature of a sample inlet is 260-280 DEG C, the initial column temperature is 40-50 DEG C, the conditions are kept for 1-5 minutes, and the temperature is increased to 200-280 DEG C at the speed of 5-30 DEG C / min; the conditions of the mass spectrum are as follows: the temperature of an ion source is 230-250 DEG C, and the temperature of a transmission line is 310 DEG C. By controlling extraction, chromatography and mass spectrometry conditions, N-methylaniline can be effectively separated from a sample, and accurate and efficient qualitative and quantitative analysis can be performed on N-methylaniline.

The application discloses a detection method for determining the residual amount of easily hydrolyzed impurities in apremilast, which comprises the following steps: using a high performance liquid chromatograph, adopting polysaccharide derivatives as a filler (CHIRALPAK IH, 4.6mm*150mm, 5um), adopting a n-hexane-tetrahydrofuran (65:35) mobile phase, a flow rate of 1.0ml per minute, a column temperature of 30 DEG C, a detection wavelength of 230nm, and a sample injection volume of 50ul. The application adopts the polysaccharide derivative filler to determine the easily hydrolyzed impurities in the apremilast, has high separation efficiency, strong anti-interference ability, fast analysis speed and high detection sensitivity, and can effectively control the quality of the apremilast by determining the amount of the easily hydrolyzed impurities in the apremilast.

The invention relates to the technical field of liquid chromatographs, in particular to a column oven centralized control method and system and a control circuit of a column oven. The method comprises the following steps: an upper computer obtains current temperature values of column temperature boxes in a plurality of liquid chromatographs in each group in real time through the Internet of Things; the upper computer determines a difference value distribution mode between the current temperature values of all the column temperature boxes in the current group and a preset target temperature value based on the current temperature values of the column temperature boxes in the plurality of liquid chromatographs in each group; if the difference value distribution mode meets a first preset condition, the upper computer starts a centralized cooperative control mode; and if the difference value distribution mode meets a second preset condition, the upper computer starts a decentralized autonomous control mode. According to the invention, the temperature control efficiency of a plurality of liquid chromatographs can be improved, and the energy consumption cost can be effectively reduced and controlled; meanwhile, the device also has an over-temperature protection function; in addition, when liquid leakage is detected, heating can be automatically stopped, and an alarm is given to ensure safety.

The present application relates to the technical field of health product ingredient detection, in particular to a detection method of a new type of sildenafil compound in health food, comprising the following steps: qualitative and quantitative detection by HPLC-MS / MS; the chromatographic conditions include: a chromatographic column, a C18 column; a column temperature, 40 DEG C; a mobile phase A, 0.1% formic acid aqueous solution; a mobile phase B, acetonitrile; a flow rate, 0.5 mL / min; a gradient elution program, 0~2.0 min, 10%B~95%B; 2.0~5.0 min, 95%B; 5.0~5.1 min, 95%B~5%B; 5.1~8.0 min, 5%B; a sample injection amount, 5 µL; the mass spectrometry conditions include: an ionization mode, an electrospray ionization source, a positive ion mode; an electrospray voltage, 5500 V; an ion source temperature, 550 DEG C; an atomization gas pressure, 345 kPa; an auxiliary gas pressure, 414 kPa; a curtain gas pressure, 276 kPa; a declustering voltage, 100 V; a collision energy, 45 V; a quantitative ion pair, 449 / 243; a qualitative ion pair, 449 / 259; the residence time of each ion pair, 250 ms; the new type of sildenafil compound contains a methyl piperazine group. The detection method of the present application is simple in operation, short in time consumption, high in sensitivity and good in stability.

The invention establishes a high performance liquid chromatography analysis method for measuring benzoic acid in miconazole nitrate cream, and the method has the advantages of strong specificity, high sensitivity and accuracy, and good repeatability and durability. Chromatographic conditions are as follows: an HPLC (High Performance Liquid Chromatography) system is a simerflight U3000 high performance liquid chromatograph (UV / DAD detector) or similar equipment; a chromatographic column is a chromatographic column taking octadecylsilane chemically bonded silica as a filling agent; the mobile phase consists of A, B and C; wherein A is a phosphate buffer solution, B is acetonitrile, and C is methanol. In addition, the concentration of phosphate in the phosphate buffer solution is 0.05 mol / L, and the pH is adjusted to be 3.5-4.0; the volume ratio of A to B to C is in the range of (40-50): 35: (15-25); the detection wavelength is 230 nm; the sample size is 10 microliters; the column temperature is 30 DEG C; the flow velocity is 1.0 ml / min.

The invention discloses a determination method and application of flavonoid components in Zhikang tablets, and relates to the technical field of traditional Chinese medicine analysis and identification. In the determination method, a test solution is determined and analyzed by adopting a high performance liquid chromatography, and the conditions of the high performance liquid chromatography are as follows: a chromatographic column is a C18 chromatographic column; the column temperature is 25-30 DEG C; the detection wavelength is 210-220 nm, the sample size is 8-12 [mu] L, and the operation is carried out for 55-80 min; the flow velocity is 0.6 to 0.8 mL / min; mobile phases: an A phase acetonitrile and a B phase 0.05-0.5% phosphoric acid solution; and eluting by adopting a gradient elution procedure. According to the determination method disclosed by the invention, most impurities in the prepared test solution are removed, so that the interference of other components on a chromatographic peak is reduced; the separation degree under the chromatographic condition is good, the base line is stable, the precision is high, the stability is high, the repeatability is good, and the RSD value is low.

The utility model belongs to the technical field of liquid chromatography, and discloses a movable liquid chromatography-mass spectrometer experiment table which comprises an experiment table body, a column oven is arranged at the top of the experiment table body, an automatic sampler is arranged at the top of the column oven, a sampling groove is formed in the surface of the automatic sampler, a positioning assembly is arranged in the sampling groove, and the positioning assembly is arranged in the sampling groove. A quaternary pump is arranged at the top of the automatic sampler, a vacuum degasser is arranged at the top of the quaternary pump, a solvent bottle box is arranged at the top of the vacuum degasser, and mounting grooves are formed in four corners of the surface of the solvent bottle box. By means of the arrangement of an automatic sampler and a positioning assembly, in the using process, a worker places a reagent tube into a positioning groove, the reagent tube can extrude an arc-shaped plate, after being extruded, the arc-shaped plate can reset through a spring, and then the arc-shaped plate can clamp the reagent tube; and the efficiency of clamping the reagent tubes with different sizes is improved, and the situation that the reagent tubes fall to the ground is reduced.

The invention relates to the technical field of purity detection, in particular to a phosphatidylcholine purity detection method and system.The method comprises the steps that in the gradient elution process of a phospholipid sample solution, the elution precision index of a target experimental group is determined by means of column temperature change data and column pressure change data of a chromatographic column and flow velocity data of a mobile phase; determining the peak symmetry reflection degree by using the slope change data and the chromatographic data of the analysis peak in the chromatogram of the target experimental group; determining the peak-to-peak separation degree of the two peaks according to the position relation between the analysis peak and the adjacent peak, and determining the chromatogram optimization degree of the target experimental group according to the peak symmetry reflection degree, the peak-to-peak separation degree and the elution precision index to obtain the optimal chromatogram and the purity result of the phosphatidylcholine. According to the method, the chromatographic peak height with better precision is obtained by combining the multi-gradient elution working condition and the chromatogram expression contrastive analysis, so that the purity detection result with higher accuracy for phosphatidylcholine is obtained.

The utility model discloses a high performance liquid chromatograph convenient to operate, which comprises a chromatograph main body, a chromatographic column and a column temperature box, the column temperature box comprises a box body, a sliding chute, a fixing clamp, a sample injection joint and a sample discharge joint, the sliding chute is arranged on the inner side of the box body, and the fixing clamp is arranged on the sliding chute. The fixing clamp, the sample introduction joint and the sample discharge joint are slidably connected with the box body through the sliding grooves, the sample introduction joint is communicated with the chromatographic instrument main body through a sample introduction pipeline, and the sample discharge joint is communicated with the chromatographic instrument main body through a sample discharge pipeline. The sample inlet connector and the sample outlet connector are respectively provided with a plug connecting seat, the sample inlet connector and the sample outlet connector are respectively provided with a rotating motor, the rotating motors are used for driving the plug connecting seats to rotate, and the fixing clamps are used for preventing the chromatographic column from rotating relative to the plug connecting seats. According to the device, the rotary motor is arranged to be matched with the plug connecting seat, so that the chromatographic column can be more conveniently and reliably mounted.

The invention discloses a method for preparing a high-purity C-glycoside flavone monomer by using secondary reversed-phase semi-preparative chromatography, which comprises the following steps of: performing primary elution on bamboo leaf flavone by using a C18 chromatographic column under the conditions of set column temperature, mobile phase system and elution mode, and monitoring and collecting a primary purified product of a peak corresponding to a target component; carrying out reduced pressure concentration on products of isoorientin and orientin corresponding peaks in the first purification product to remove the solvent, redissolving residues by using an organic solvent II, carrying out second elution on a sample solution obtained by filtering through a microfiltration membrane by using a C18 chromatographic column in a set column temperature, a mobile phase system and an elution mode, and collecting eluent; monitoring and collecting a secondary purified product of a peak corresponding to the target component, and performing vacuum concentration and freeze drying. The bamboo leaf flavonoid product obtained by the method has the purity of not less than 98%, the raw material utilization rate is obviously improved, the separation efficiency is high, and the purification period is greatly shortened.

The invention belongs to the field of drug detection and analysis, and particularly relates to a method for detecting rehmannia D in radix angelicae sinensis and six-yellow decoction based on a column switching chromatography technology. The method comprises the following steps: preparing a reference solution and a test solution, positioning a target peak by using a chromatographic column 1, carrying out separation by switching a chromatographic column 2 2 min before the peak appears, and carrying out detection by using methanol-0. 1% phosphoric acid (4: 96) as a mobile phase at a flow rate of 0.20-0.25 mL / min and a column temperature of 20 DEG C. And the model number of the chromatographic column 2 is Waters HSS T3. According to the present invention, the separation degree of the detected rehmannia glutinosa libosch D is more than 1.5, the peak purity is more than 980, the peak area ratio RAD of the test sample to the reference substance is 4.1%, the specificity and the durability are good, and the requirement of the accurate detection of the rehmannia glutinosa libosch D content is met.

A method for tuning a rig site gas chromatography apparatus includes acquiring a chromatogram of a gas sample using a rig site GC apparatus at a nominal column temperature and a nominal carrier gas flow rate. The gas sample includes at least first and second light alkane gases (C1 and Cn). C1 and Cn elution times are extracted from the obtained chromatogram. An optimum column temperature is determined from the nominal column temperature and the extracted C1 and Cn elution times. An optimum carrier gas flow rate is determined from the nominal carrier gas flow rate, the nominal column temperature, the computed optimum column temperature, and the extracted C1 elution time. The GC apparatus is then configured to make GC measurements using the computed optimum column temperature and the computed optimum carrier gas flow rate.

This invention discloses an automatic high-performance liquid chromatography (HPLC) column regeneration device and its usage method, comprising a column temperature chamber and a power chamber. The column temperature chamber is equipped with a temperature control module, which houses the chromatographic column. The power chamber contains switching valves A, B, C, and D, and an infusion pump. Switching valves A, B, and C are multi-port selector valves, and switching valve D is a two-position four-port switching valve. Multiple branch interfaces of switching valve A are connected to multiple solvent bottles via pipelines. The common interface of switching valve A is connected to the infusion pump. The four interfaces of switching valve D are respectively connected to the infusion pump, switching valves B and C, the common interface, and a waste bottle. Multiple branch interfaces of switching valves B and C are respectively connected to both ends of multiple chromatographic columns. Through four switches, the flushing solvent, flushing direction, and the column to be regenerated can be automatically switched. The device is simple to operate, has low labor costs, high regeneration efficiency, and is economically beneficial.

The invention provides a method for detecting white oil and application thereof, and relates to the technical field of biologication.The method for detecting the white oil comprises the steps that the conditions of gas chromatography are set as follows: the temperature of a sample inlet is 300-320 DEG C; the temperature of the detector is 310-330 DEG C; the initial temperature is 150 DEG C, the temperature is maintained for 2 minutes, then the temperature is raised to 310 DEG C at the rate of 5 DEG C per minute, and the temperature is maintained for 10 minutes; the flow rate of sample introduction is 1.0 mL / min, and split-flow sample introduction is adopted. According to the method, the white oil is detected by adopting the gas chromatography, the method for detecting the white oil, which is rapid, stable and good in repeatability, is established by adopting specific gas chromatography conditions, the components of the white oil can be effectively separated, the area percentage of each component can be measured, the carbon chain composition of each white oil can be more rapidly and intuitively compared according to the detection result, and the detection result is more accurate. Furthermore, the key technical problem of quality control of the white oil raw material for injection of poultry vaccines can be solved.

The application discloses a detection method of indole-3-methanal in compound amino acid injection, and belongs to the field of analytical chemistry. The detection method adopts high performance liquid chromatography, and the chromatographic conditions include: a chromatographic column with octadecylsilane bonded silica as a filler, isocratic elution with a mobile phase, detection by a UV detector with a detection wavelength of 301-311 nm, a flow rate of the mobile phase of 0.6-1.0 mL / min, a sample injection amount of 50-150 muL, a column temperature of 30-40 DEG C, and a mobile phase of a mixed solution of acetonitrile and phosphate buffer solution. The application selects the proportion of the mobile phase, pH, flow rate, column temperature, wavelength and sample injection amount and other chromatographic conditions in the light of the low response and small polarity of the impurity by using high performance liquid chromatography, and the method is verified systematically, and has the advantages of strong specificity, high sensitivity, good precision, strong durability and the like, and can qualitatively or quantitatively detect indole-3-methanal in the compound amino acid injection, so that the effectiveness and safety of the related products are improved.

The invention relates to a chromatographic instrument temperature monitoring device, and belongs to the technical field of chemical analysis equipment, the structure of the temperature monitoring device comprises a column oven, the top of the column oven is provided with a first connection port, and the first connection port in the column oven is provided with a communication pipeline; a second connecting port is formed in the bottom of the column oven; a monitoring chamber is arranged in the middle of the communicating pipeline, one end of the monitoring chamber is communicated with the communicating pipeline, and the other end of the monitoring chamber is arranged outside the column oven in a penetrating manner; a chromatographic column is arranged in the column oven, the top end of the chromatographic column is arranged on the first connector through a communicating pipeline, and the bottom end of the chromatographic column is connected with the second connector; a heating assembly is arranged on the inner wall of the column oven; a circulating air cooling assembly is arranged on one surface of the column oven; the method has the technical effect of adapting to multi-working-condition composite fault monitoring requirements.

The invention relates to the technical field of chemical analysis, in particular to a gas phase detection method of 3-amino-4 '-nitrodiphenyl ether, which is characterized by comprising the following steps: S1, preparation of standard solutions: taking a 3-amino-4'-nitrodiphenyl ether standard substance, and preparing a series of standard solutions with different concentrations by using acetonitrile; preparing a sample solution: taking a 3-amino-4 '-nitrodiphenyl ether sample, and preparing the sample solution by using a solvent acetonitrile; s2, performing gas chromatography detection on the standard solution and the sample solution according to the following gas chromatography conditions: a detector is a hydrogen flame ionization detector; a chromatographic column is a high-temperature chromatographic column; column temperature: temperature programming; the temperature of a sample inlet is 280-300 DEG C; the temperature of a detector is 300 DEG C to 310 DEG C; the split ratio is (80-100): 1. The detection method has the advantages of stable base line, no trailing, good peak pattern, low instrument operation cost, simple and convenient detection process and good repeatability.

The invention discloses a method for detecting related substances of calcium gluconate. The detection method is a high performance liquid chromatography-electric fog type detection method. Chromatographic conditions are as follows: a chromatographic column takes cation exchange resin as a filler, the evaporation temperature is 50-60 DEG C, the collection frequency is 8-12 Hz, the filtration constant is 4.5-5.5 s, the power function value is 1.8-2.2, the column temperature is 45-55 DEG C, the mobile phase is 0.09-0.11% trifluoroacetic acid solution, and isocratic elution is performed. The method is used; d-glucuronic acid, isomaltohexanoic acid, maltose acid, fructose, D-glucuronic acid lactone, glucose, D-gluconic acid-gamma-lactone, glucaric dialdehyde, D-gluconic acid-delta-lactone, oxalic acid, maltotriose, 5-hydroxymethyl-2-furancarboxylic acid, 5-hydroxymethyl-2-furancarboxylic acid, 5-hydroxymethyl-2-furancarboxylic acid, 5-hydroxymethyl-2-furancarboxylic acid, 5-hydroxymethyl-2-furancarboxylic acid, 5-hydroxymethyl-2-furancarboxylic acid, 5-hydroxymethyl-2- According to the method, impurities such as 3, 5-hydroxymethyl-2-furaldehyde and the like are well separated from calcium gluconate, and the impurities in the method are relatively good in sensitivity, relatively small in baseline interference, relatively good in specificity and the like, so that the defects in the prior art can be effectively overcome, and related substances in the calcium gluconate can be effectively controlled; the safety, the effectiveness and the quality controllability of various calcium supplements prepared by taking the calcium gluconate as the raw material medicine are ensured.

A method for tuning a drilling platform gas chromatography apparatus includes acquiring a chromatogram of a gas sample using a drilling platform GC apparatus at a nominal column temperature and a nominal carrier gas flow rate. The gas sample comprises at least a first light alkane gas and a second light alkane gas (C1 and Cn). And extracting the C1 elution time and the Cn elution time from the obtained chromatogram. And determining an optimal column temperature according to the nominal column temperature, the extracted C1 elution time and the extracted Cn elution time. An optimal carrier gas flow rate is determined as a function of the nominal carrier gas flow rate, the nominal column temperature, the calculated optimal column temperature, and the extracted C1 elution time. The GC device is then configured to make GC measurements using the calculated optimal column temperature and the calculated optimal carrier gas flow velocity.