85 results about "Column temperature" patented technology

The invention relates to a chromatographic instrument temperature control method and system, an intelligent terminal and a storage medium, and relates to the technical field of chromatographic instrument sample separation.The method comprises the steps that the sample type of a preset sample is obtained; analyzing the sample type to determine a window heating rate and a window target temperature; acquiring a chromatographic column initial temperature of a preset chromatographic column; analyzing the initial temperature of the chromatographic column and the target temperature of the window to determine the temperature of the chromatographic column needing to be increased; analyzing the temperature required to be increased and the window heating rate of the chromatographic column so as to determine the heating time of the chromatographic column; and controlling a preset chromatographic column temperature regulation and control device to perform temperature control on the chromatographic column according to the chromatographic column heating time and the window target temperature. The method has the effect of improving the precision of the temperature retention time.

The invention relates to the technical field of food safety detection, in particular to a method for detecting urea in bean sprouts and application thereof.The method comprises the following steps that smashed bean sprouts are subjected to two times of ultrasonic extraction and centrifugation, supernate is combined, and an extracting solution is obtained; fixing the volume and filtering to obtain a to-be-detected solution; detecting the liquid to be detected by using liquid chromatography-tandem mass spectrometry; an extraction solvent for ultrasonic extraction is a 5% formic acid-acetonitrile composite solution; the conditions of the liquid chromatography-tandem mass spectrometry are as follows: the chromatographic conditions are as follows: a chromatographic column is an HILIC column; the sample size is 10 [mu] L; a mobile phase is a mixed solution of a 0.02 mmol / L ammonium acetate buffer salt solution and acetonitrile, and isocratic elution is carried out; the flow rate is 0.3 mL / min; the column temperature is 40 DEG C; mass spectrum conditions are as follows: an electrospray ionization (ESI) positive ion mode, electrospray voltage of 4000 V, sheath gas pressure of 30 psi, auxiliary gas pressure of 5 psi, capillary heating temperature of 300 DEG C and scanning mode of MRM. The method disclosed by the invention is free from substance interference, high in sensitivity, accurate in result, low in cost and high in efficiency, and can be used as a standard detection method.

This utility model relates to a column housing device for housing a chromatographic column within a column oven. The device includes: a housing with a base having a first side and an opposite second side, wherein the first side has a groove; a first sidewall and a second sidewall extending from the first side of the base, wherein the second sidewall is arranged opposite to the first sidewall; and a support member including a bracket disposed in the groove, such that the support member is detachably fixed to the first side of the base via the bracket between the first and second sidewalls, thereby forming a heat exchange channel between the base, the support member, and the first and / or second sidewalls for placing the chromatographic column. This column housing device detachably connects the support member to the housing, providing a flexible and adjustable thermal structure that balances column placement stability and heat transfer efficiency. This utility model also relates to a column oven and a chromatogram.

The invention provides a system and method for detecting the content of a corrosion inhibition additive in cooling liquid, specifically, the system comprises a liquid chromatograph, a diode array detector DAD and an electric fog detector CAD which are sequentially connected in series, the liquid chromatograph comprises a mobile phase pump, an automatic sampler and a column oven, and a chromatographic column is arranged in the column oven; wherein the mobile phase pump is used for mixing different mobile phases according to a preset proportion and then conveying the mixed mobile phases to the automatic sample injector; the automatic sample injector is used for injecting the mobile phase from the mobile phase pump into the to-be-detected cooling liquid and flushing the to-be-detected cooling liquid injected with the mobile phase to the chromatographic column; the column oven is used for controlling the column temperature of the chromatographic column; the chromatographic column is used for performing chromatographic separation on the corrosion inhibition additive in the to-be-detected cooling liquid; the DAD is used for detecting the corrosion inhibition additive containing the benzene ring in the cooling liquid to be detected; the CAD is used for detecting the polybasic aliphatic carboxylic acid type corrosion inhibition additive in the cooling liquid to be detected.

The application relates to the technical field of data processing, and provides a method for calculating a temperature field, which comprises the following steps: determining a first density of first column temperature sampling points; determining the number A of temperature sampling points between a first temperature sensor and a second temperature sensor according to a first interval between the first temperature sensor and the second temperature sensor and the first density; determining A temperature values through interpolation operation according to temperature values of the first temperature sensor and the second temperature sensor; determining a second density of first row temperature sampling points; determining the number B of temperature sampling points between the first temperature sampling points and the second temperature sampling points according to a second interval between the first temperature sampling points and the second temperature sampling points and the second density; determining B temperature values through interpolation operation according to temperature values of the first temperature sampling points and the second temperature sampling points; and determining the temperature field of a machine room according to the A temperature values and the B temperature values. The method can improve the efficiency of calculating the temperature field.

The invention relates to a method for analyzing and detecting a non-azonetin enantiomer, which adopts normal-phase high performance liquid chromatography and takes normal hexane-ethanol-trifluoroacetic acid as a mobile phase to detect the non-azonetin enantiomer, and the specific chromatographic conditions are as follows: a chromatographic column is prepared by covalently bonding amylose-tri (3, 4, 6-trimethyl-1, 3-pentanediol) on the surface of silica gel; 2, 5-dichlorophenyl carbamate is used as a filling agent (IE, 4.6 * 250 mm, 5.0 [mu] m); a mobile phase: n-hexane: ethanol: trifluoroacetic acid = 500: 500: 1 (V / V / V); the flow rate is 1.0 ml / min; the column temperature is 30 DEG C; the sample size is 20 [mu] l; the detection wavelength is 280 nm; the elution gradient is isocratic elution. The invention provides a high performance liquid chromatography method for analyzing the enantiomer of the fiazonetin, the complete separation of the fiazonetin and the enantiomer of the fiazonetin can be realized by adopting the method, and the separation degree of a main component and the enantiomer is greater than 1.5.

The invention relates to the technical field of biological analysis, in particular to a fish and crustacean biological analysis device which comprises a placing frame and a mobile phase solvent bottle placed in the upper portion of the placing frame, a vacuum degassing box is connected to the lower portion of the placing frame, and a high-pressure pump box is connected to the lower portion of the vacuum degassing box. A sample injection box is connected to the lower part of the high-pressure pump box, a detection box is connected to the lower part of the column oven, an automatic sample injector is arranged in the sample injection box above the placing frame, and a chromatographic column is connected to the interior of the column oven. According to the biological analysis device for the fishes and the crustaceans, through slight shaking in multiple directions, the uniform shaking efficiency and effect of a sample solution in the sample solvent bottle can be improved, the situation that particles settle to the bottom of the bottle and are layered due to long-time standing of the sample solvent bottle is avoided, the concentration of the sample solution sucked by an automatic sample injector is uniform, and the accuracy of the sample solution is improved. Therefore, the accuracy of the detection result of the biological analysis device can be improved, and the biological analysis work of fishes and crustaceans cannot be influenced.

The utility model discloses a column temperature box with automatic chromatographic column selection system, including the box, the box surface is equipped with the door body of rotation connection, is equipped with the valve cut system in the box to the door body one side, and the valve cut system includes the mounting panel in the box top, the chromatographic column installation valve head on the mounting panel, the control valve in the chromatographic column installation valve head tail portion, is equipped with the temperature control area below the valve cut system, and the temperature control baffle that installs in the box is equipped in the temperature control area, and the mounting bracket on the surface of temperature control baffle and the chromatographic column on the mounting bracket, the bottom of temperature control area still is equipped with the liquid leakage device.

The invention discloses a method for detecting 5 '(E)-VPA phosphoramidite monomer related substances, and belongs to the technical field of liquid chromatography detection. Related substances comprise 5 '-(E)-VP-2'-OMe-A (Bz), 5 '(Z)-VPA, 5'-(E)-VP-2 '-OMe-A-CE-P and 5' (E)-VPA-OX, and chromatographic detection conditions comprise that the column temperature is 23-27 DEG C, a stationary phase of a chromatographic column is octadecyl bonded silica gel, a mobile phase A is an ammonium acetate solution, a mobile phase B is acetonitrile, gradient elution is performed, and the like. According to the method, four related substances in the 5 '(E)-VPA phosphoramidite monomer are effectively separated through specific liquid chromatography conditions, the system adaptability, specificity, linearity and range, detection limit, quantitation limit and accuracy verification of the method all meet acceptable standards of pharmacopoeia, and detection of multiple related substances can be completed within a short time; and a reliable means is provided for detecting and controlling the quality of the 5 '(E)-VPA phosphoramidite monomer.

The invention belongs to the technical field of gas chromatography detection, and relates to a gas chromatography method for simultaneously determining the content of ethanol and isopropyl myristate. The set conditions of the gas chromatography are as follows: the temperature of an FID detector and a polyethylene glycol 20M capillary column is 240-260 DEG C, the temperature of the detector is 240-260 DEG C, the carrier gas is nitrogen, the flow rate is 0.9-2.5 mL / min, the sample injection amount is 1 mu L, the column temperature is increased by adopting a program, the initial temperature is 80-90 DEG C, the temperature is increased to 180-190 DEG C at the rate of 9-12 DEG C / min, and the temperature is maintained for at least 10 minutes. The method provided by the invention can realize simultaneous detection of ethanol and isopropyl myristate, after the two substances are dissolved by the N, N-dimethylformamide solvent, the detected spectrogram does not have the phenomena of peak overlapping, trailing and the like, and the method has the advantages of high separation efficiency, high selectivity, high sensitivity, high analysis speed and wide application range.

The invention discloses a method for determining the content of poloxamer 124 in the technical field of medical analysis. Liquid chromatography is adopted for detection, and a gel chromatographic column filled with porous silicon dioxide is selected as a chromatographic column; a mobile phase is a trifluoroacetic acid-acetonitrile-water solution; the weight ratio of the mobile phase trifluoroacetic acid to acetonitrile to water is 0.1: (18-22): (78-82); the column temperature is 25-45 DEG C; the temperature of a detector is 60-80 DEG C; the flow velocity of the mobile phase is 0.6-1.0 ml / min; the sample size is 5-20 [mu] l, automatically injecting the sample according to the chromatographic conditions, recording the peak area, and calculating the content according to an area normalization method. The method is high in response value, simple in detection operation and high in product content accuracy, specificity, repeatability, sampling precision, linearity and range all meet the requirements, and the quantitative determination problem of poloxamer 124 is solved.

The invention provides a method for determining D-psicose in processed food and application of the method. The method specifically comprises the following steps: extracting a sample with warm water at 50-60 DEG C, precipitating protein by adopting a Carrez reagent, and removing interference impurities; chromatographic conditions are defined as follows: a calcium type strong cation exchange column is adopted, pure water containing 50 mg / L of EDTA-Ca is adopted as a mobile phase, the flow rate is 0.4-0.6 mL / min, and the column temperature is 80 DEG C. The result shows that the linear relation of the D-psicose is good in the concentration range of 1.0 mg / mL to 20.0 mg / mL, and the correlation coefficient is 0.999999; according to the method, the LOD is 0.002 g / 100g, and the LOQ is 0.005 g / 100g. Under the experimental conditions of standard addition before simulation processing, the average recovery rate of 18 matrixes including dairy products, bakery products and the like is 81.5-128.1%, and the RSD is less than 3.0%. The determination method provided by the invention is simple in pretreatment, high in sensitivity, strong in interference resistance and good in accuracy, effectively solves the ubiquitous problems of matrix wrapping, matrix interference, difficulty in isomer separation and the like when the D-psicose in the processed food is detected, can realize rapid and accurate detection of the D-psicose in all dosage forms of processed food, and has a wide prospect.

The invention discloses a method for improving the detection recovery rate of ascorbyl palmitate in a compound product, which comprises the following steps: taking a sample, adding BHT (butylated hydroxytoluene), then adding an isopropanol-n-hexane mixed solution containing 0.8-1.2% by volume of glacial acetic acid as an extraction solvent, then fully mixing, extracting, freezing, centrifuging, taking supernate, and collecting the supernate to obtain the ascorbyl palmitate. The chromatographic conditions are as follows: a C18 reversed-phase chromatographic column is adopted, a mobile phase A and a mobile phase B are used for gradient elution, the flow velocity of the mobile phase is 0.8-1.2 mL / min, the column temperature is 28-32 DEG C, and the maximum absorption wavelength of ascorbyl palmitate is taken as the detection wavelength. According to the method, the extraction efficiency is improved by optimizing a sample pretreatment process, and the problem that the liquid phase detection error of the substance in the compound product is generally higher than 10% is effectively solved in combination with improved high performance liquid chromatography separation conditions.

The invention relates to the technical field of polypeptide detection, in particular to a method for detecting the content of a semeglutide polymer. A liquid chromatography method is adopted, wherein a chromatographic column is G2000 SWxl; carrying out isocratic elution for 40 minutes; the detection wavelength is 280 nm; the column temperature is 26-30 DEG C; the mobile phase is formed by mixing a phosphate solution of sodium chloride and isopropanol according to the volume ratio of 50: 50. The selected mobile phase has no influence on the retention performance of the chromatographic column, the separation degree of the polymer and the main peak is greater than 1.5, and the requirement on an instrument is lower; accurate and reliable experimental results can be obtained by determining the polymer in the semeglutide through the method.

The invention belongs to the technical field of pharmaceutical analysis, and discloses a liquid chromatography detection method of a pimobendan intermediate, which comprises the following steps: injecting octadecyl bond and silica gel as a stationary phase, a phosphate buffer solution with the pH value of 3.0-5.0 as a water phase and methanol (or acetonitrile) as an organic phase into a high performance liquid chromatograph under the conditions that the column temperature is 30-60 DEG C, the sample injection is 5-30 mu L and the flow velocity is 0.5-2 ml / min, and carrying out gradient elution to obtain the pimobendan intermediate. And the sample is analyzed in a wavelength range of 200-230nm. By adjusting the mobile phase system and optimizing the mobile phase gradient, a main peak and an impurity peak are completely separated, the analysis speed is high, the detection sensitivity is high, and the stability and the quality of a product in production can be better detected.

The invention relates to the technical field of drug analysis and detection, and provides a method for detecting 1, 2, 4-triazole in fluconazole sodium chloride injection, which adopts high performance liquid chromatography. The specific conditions of the high performance liquid chromatography are as follows: a solvent is a water-acetonitrile mixed solution with the volume ratio of 95: 5; the mobile phase comprises a mobile phase A and a mobile phase B, the mobile phase A is water, and the mobile phase B is acetonitrile; the flow velocity is 1.0 mL / min; octadecylsilane chemically bonded silica is used as a filling agent of a chromatographic column; the column temperature is 30 DEG C; the sample injection volume is 100 microliters; performing gradient elution; and the detection wavelength is 195 nm. The method has the advantages that the content of 1, 2, 4-triazole in the fluconazole sodium chloride injection can be quantitatively and accurately determined without derivation reaction, the unification of operation convenience and detection accuracy is realized, and reliable technical support is provided for quality control of the fluconazole sodium chloride injection.

The application discloses a method for improving the chromatographic separation degree of impurities A and B in acetylcysteine raw materials, and is characterized by the following steps: controlling the column temperature of a chromatographic column within a specific low temperature range of 15 DEG C plus or minus 2 DEG C, and using the difference between the distribution behaviors of impurities A and B between a stationary phase and a mobile phase at different temperatures to significantly improve the separation degree of the two impurities. When the column temperature is 15 DEG C, the separation degree of impurities A and B can reach 2.06, which is much higher than the pharmacopoeia standard, and the separation degrees under the conditions of 30 DEG C, 35 DEG C and 40 DEG C all fail to meet the standard. The application does not need to replace the chromatographic column, is simple and convenient to operate, low in cost, can stably meet the accurate quantification requirements of related substances in acetylcysteine raw materials, and has good popularization and application value.

The invention provides a method for detecting methyl lactate in a sodium lactate solution, a gas chromatography is combined with a headspace sampling technology, and chromatographic conditions are as follows: a chromatographic column is a capillary column taking polyethylene glycol or a compound with similar polarity as a stationary liquid; a sample inlet is 180 DEG C, and headspace sample injection is performed; a flame ionization detector is adopted, and the temperature is 230 DEG C; the carrier gas is nitrogen, the flow velocity is 2.0 ml / min, and the split ratio is 10: 1; the column temperature program is as follows: 35 DEG C is maintained for 3 minutes, the temperature is raised to 90 DEG C at 5 DEG C / min and maintained for 3 minutes, and then the temperature is raised to 200 DEG C at 15 DEG C / min and maintained Headspace conditions are as follows: the balance temperature of a headspace bottle is 90 DEG C, and the time is 30 minutes; a quantitative loop is 100 DEG C; the temperature of the transmission line is 110 DEG C; the circulation time is 40 minutes. The method can accurately and quantitatively detect methyl lactate, is convenient to operate, and has great significance in improving the quality control level of the sodium lactate solution, guaranteeing the application safety of the sodium lactate solution in the fields of medicines and foods and perfecting a quality standard system.

The invention relates to the technical field of analysis and detection, in particular to a gas chromatography-mass spectrometry detection method for N-methylaniline. The gas chromatography-mass spectrometry detection method for N-methylaniline comprises the following steps: treating a sample to be neutral, and extracting N-methylaniline in the sample by using dichloromethane to obtain a liquid to be detected; aa, separating the to-be-detected liquid through a quartz capillary column, and carrying out gas chromatography-mass spectrometry detection; the conditions of the gas chromatography are as follows: the temperature of a sample inlet is 260-280 DEG C, the initial column temperature is 40-50 DEG C, the conditions are kept for 1-5 minutes, and the temperature is increased to 200-280 DEG C at the speed of 5-30 DEG C / min; the conditions of the mass spectrum are as follows: the temperature of an ion source is 230-250 DEG C, and the temperature of a transmission line is 310 DEG C. By controlling extraction, chromatography and mass spectrometry conditions, N-methylaniline can be effectively separated from a sample, and accurate and efficient qualitative and quantitative analysis can be performed on N-methylaniline.

The application discloses a detection method for determining the residual amount of easily hydrolyzed impurities in apremilast, which comprises the following steps: using a high performance liquid chromatograph, adopting polysaccharide derivatives as a filler (CHIRALPAK IH, 4.6mm*150mm, 5um), adopting a n-hexane-tetrahydrofuran (65:35) mobile phase, a flow rate of 1.0ml per minute, a column temperature of 30 DEG C, a detection wavelength of 230nm, and a sample injection volume of 50ul. The application adopts the polysaccharide derivative filler to determine the easily hydrolyzed impurities in the apremilast, has high separation efficiency, strong anti-interference ability, fast analysis speed and high detection sensitivity, and can effectively control the quality of the apremilast by determining the amount of the easily hydrolyzed impurities in the apremilast.

The present application relates to a method for removing the main peak residue of liraglutide precursor in a chromatographic column, and the liquid chromatography conditions are as follows: equipment configuration: four-element low-pressure pump or two-element high-pressure pump equipped with flow path switching valve; chromatographic column: InertSil ODS-SP chromatographic column (4.6*250mm, 5um); column temperature: 35-40 DEG C; mobile phase C: sulfate buffer: acetonitrile (82:18), 8M NaOH is adjusted to pH 5.95-6.05; mobile phase D: 50% acetonitrile; flow rate: 1.0ml / min; detection wavelength: 214nm; injection mode: 60% mobile phase D and 40% mobile phase C, isocratic elution for 8min, 0.01M phosphate buffer is injected, 30ul / needle, 1 needle is injected every 8min, a total of 3 needles. The analysis method of the present application can completely remove the main peak residue of liraglutide precursor in the chromatographic column in a short time.

The invention relates to the technical field of liquid chromatographs, in particular to a column oven centralized control method and system and a control circuit of a column oven. The method comprises the following steps: an upper computer obtains current temperature values of column temperature boxes in a plurality of liquid chromatographs in each group in real time through the Internet of Things; the upper computer determines a difference value distribution mode between the current temperature values of all the column temperature boxes in the current group and a preset target temperature value based on the current temperature values of the column temperature boxes in the plurality of liquid chromatographs in each group; if the difference value distribution mode meets a first preset condition, the upper computer starts a centralized cooperative control mode; and if the difference value distribution mode meets a second preset condition, the upper computer starts a decentralized autonomous control mode. According to the invention, the temperature control efficiency of a plurality of liquid chromatographs can be improved, and the energy consumption cost can be effectively reduced and controlled; meanwhile, the device also has an over-temperature protection function; in addition, when liquid leakage is detected, heating can be automatically stopped, and an alarm is given to ensure safety.

The present application relates to the technical field of health product ingredient detection, in particular to a detection method of a new type of sildenafil compound in health food, comprising the following steps: qualitative and quantitative detection by HPLC-MS / MS; the chromatographic conditions include: a chromatographic column, a C18 column; a column temperature, 40 DEG C; a mobile phase A, 0.1% formic acid aqueous solution; a mobile phase B, acetonitrile; a flow rate, 0.5 mL / min; a gradient elution program, 0~2.0 min, 10%B~95%B; 2.0~5.0 min, 95%B; 5.0~5.1 min, 95%B~5%B; 5.1~8.0 min, 5%B; a sample injection amount, 5 µL; the mass spectrometry conditions include: an ionization mode, an electrospray ionization source, a positive ion mode; an electrospray voltage, 5500 V; an ion source temperature, 550 DEG C; an atomization gas pressure, 345 kPa; an auxiliary gas pressure, 414 kPa; a curtain gas pressure, 276 kPa; a declustering voltage, 100 V; a collision energy, 45 V; a quantitative ion pair, 449 / 243; a qualitative ion pair, 449 / 259; the residence time of each ion pair, 250 ms; the new type of sildenafil compound contains a methyl piperazine group. The detection method of the present application is simple in operation, short in time consumption, high in sensitivity and good in stability.

The invention establishes a high performance liquid chromatography analysis method for measuring benzoic acid in miconazole nitrate cream, and the method has the advantages of strong specificity, high sensitivity and accuracy, and good repeatability and durability. Chromatographic conditions are as follows: an HPLC (High Performance Liquid Chromatography) system is a simerflight U3000 high performance liquid chromatograph (UV / DAD detector) or similar equipment; a chromatographic column is a chromatographic column taking octadecylsilane chemically bonded silica as a filling agent; the mobile phase consists of A, B and C; wherein A is a phosphate buffer solution, B is acetonitrile, and C is methanol. In addition, the concentration of phosphate in the phosphate buffer solution is 0.05 mol / L, and the pH is adjusted to be 3.5-4.0; the volume ratio of A to B to C is in the range of (40-50): 35: (15-25); the detection wavelength is 230 nm; the sample size is 10 microliters; the column temperature is 30 DEG C; the flow velocity is 1.0 ml / min.

The application discloses a multi-component content detection method for a cold heat-clearing preparation, and comprises the following steps: setting chromatographic conditions, detecting a wavelength of 250 nm, setting a column temperature to 30 DEG C, taking C18 as a chromatographic column, taking a methanol (A)-water (B) system as a mobile phase system, gradient elution, and setting a program as follows: 0-10 min, 5%-20% A; 10-40 min, 20%-30% A; 40-50 min, 30%-80% A; 50-65 min, 80%-95% A; respectively sucking test sample solutions and mixed control samples for testing, and calculating 3'-hydroxymethyl puerarin, puerarin, 3'-methoxymethyl puerarin and apiosyl puerarin glycoside contents of the cold heat-clearing granules according to a chromatogram. The cold heat-clearing preparation component content is simultaneously detected by the application, the result is accurate, comprehensive and stable, and the application is beneficial to comprehensively and rapidly checking the product quality.

The invention discloses a determination method and application of flavonoid components in Zhikang tablets, and relates to the technical field of traditional Chinese medicine analysis and identification. In the determination method, a test solution is determined and analyzed by adopting a high performance liquid chromatography, and the conditions of the high performance liquid chromatography are as follows: a chromatographic column is a C18 chromatographic column; the column temperature is 25-30 DEG C; the detection wavelength is 210-220 nm, the sample size is 8-12 [mu] L, and the operation is carried out for 55-80 min; the flow velocity is 0.6 to 0.8 mL / min; mobile phases: an A phase acetonitrile and a B phase 0.05-0.5% phosphoric acid solution; and eluting by adopting a gradient elution procedure. According to the determination method disclosed by the invention, most impurities in the prepared test solution are removed, so that the interference of other components on a chromatographic peak is reduced; the separation degree under the chromatographic condition is good, the base line is stable, the precision is high, the stability is high, the repeatability is good, and the RSD value is low.

The invention discloses a method for detecting vitamin B12 and impurities thereof, and belongs to the technical field of medicine analysis and detection. The method comprises the following steps: introducing a sample to be detected into high performance liquid chromatography for detection; high performance liquid chromatography detection conditions are as follows: a chromatographic column takes hydrophilic octadecylsilane chemically bonded silica as a filler; the mobile phase comprises a mobile phase A and a mobile phase B, the mobile phase A is a buffer solution, and the mobile phase B is methanol; the pH value of the buffer solution is 3-4; the column temperature is 55-65 DEG C; the flow velocity of the mobile phase is 0.15 to 0.25 mL / min; the sample injection volume is 15-25 [mu] L; the detection wavelength is 361nm; the elution mode is isocratic elution; the volume ratio of the mobile phase A to the mobile phase B in isocratic elution is (80-90): (10-20). The method has good specificity, accuracy, precision and durability, can realize single detection of vitamin B12 and impurities thereof, and is simple, convenient and feasible.

The utility model belongs to the technical field of liquid chromatography, and discloses a movable liquid chromatography-mass spectrometer experiment table which comprises an experiment table body, a column oven is arranged at the top of the experiment table body, an automatic sampler is arranged at the top of the column oven, a sampling groove is formed in the surface of the automatic sampler, a positioning assembly is arranged in the sampling groove, and the positioning assembly is arranged in the sampling groove. A quaternary pump is arranged at the top of the automatic sampler, a vacuum degasser is arranged at the top of the quaternary pump, a solvent bottle box is arranged at the top of the vacuum degasser, and mounting grooves are formed in four corners of the surface of the solvent bottle box. By means of the arrangement of an automatic sampler and a positioning assembly, in the using process, a worker places a reagent tube into a positioning groove, the reagent tube can extrude an arc-shaped plate, after being extruded, the arc-shaped plate can reset through a spring, and then the arc-shaped plate can clamp the reagent tube; and the efficiency of clamping the reagent tubes with different sizes is improved, and the situation that the reagent tubes fall to the ground is reduced.

The application relates to the technical field of drug detection, in particular to an HPLC detection method for enantiomers in letimovir injection, which comprises the following steps: preparing letimovir sample solution; preparing control sample solution; and detecting by adopting a reversed-phase high performance liquid chromatography system, wherein the high performance liquid chromatography conditions are as follows: a chromatographic column filled with ovomucoid bonded silica gel; a mobile phase A: 0.01 mol / L phosphate solution with a pH value of 3.8-4.2; a mobile phase B: a mixed solution of methanol and acetonitrile, wherein the volume ratio of the methanol to the acetonitrile is 90:10; a flow rate of 0.35-0.45 ml / min; a column temperature of 32-38 DEG C; and a detection wavelength of 250-260 nm. The letimovir and isomers thereof can be effectively separated by the application, the separation capacity is high, the chromatographic medium performance is stable, and the operation process is simple.

The invention relates to the technical field of purity detection, in particular to a phosphatidylcholine purity detection method and system.The method comprises the steps that in the gradient elution process of a phospholipid sample solution, the elution precision index of a target experimental group is determined by means of column temperature change data and column pressure change data of a chromatographic column and flow velocity data of a mobile phase; determining the peak symmetry reflection degree by using the slope change data and the chromatographic data of the analysis peak in the chromatogram of the target experimental group; determining the peak-to-peak separation degree of the two peaks according to the position relation between the analysis peak and the adjacent peak, and determining the chromatogram optimization degree of the target experimental group according to the peak symmetry reflection degree, the peak-to-peak separation degree and the elution precision index to obtain the optimal chromatogram and the purity result of the phosphatidylcholine. According to the method, the chromatographic peak height with better precision is obtained by combining the multi-gradient elution working condition and the chromatogram expression contrastive analysis, so that the purity detection result with higher accuracy for phosphatidylcholine is obtained.