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81 results about "Triterpenoid" patented technology

Triterpenoids are terpenoid derivatives of triterpene molecules. Notable examples Betulinic acid Sterols Triterpenoid saponins

Composite capsule with synergistic stomach-nourishing and anti-inflammatory effects and preparation method thereof

The invention discloses a composite capsule with synergistic stomach-nourishing and anti-inflammatory effects and a preparation method thereof, and relates to the technical field of functional foods, health care products and medicines. The lactic acid bacteria, the egg yolk globulin and the dandelion extract are used as main raw materials to prepare the composite capsule, the ratio of the raw materials is optimized, the three raw materials in the composite capsule generate a synergistic effect, triterpenoid components in the dandelion extract can inhibit helicobacter pylori, and the triterpenoid components, the lactic acid bacteria and the egg yolk globulin jointly repair gastrointestinal mucosa, so that the gastric mucosa is inhibited, and the gastric mucosa is prevented from being damaged. Gastrointestinal health is synergistically regulated, and resistance is enhanced; meanwhile, the dandelion extract and the egg yolk globulin serve as protective carriers, the survival rate of the lactic acid bacteria in gastric acid is increased, and experimental data shows that the survival rate is increased to 81% from 30%; compared with a single formula, the compound capsule can increase the survival rate of lactic acid bacteria and increase the bioavailability of the dandelion extract by enhancing the immune effect of egg yolk globulin.
Owner:JIANGSU TIANPING PHARM CO LTD

Triterpenoid synthase EhypOSC3 gene of euphorbia lactuca and application of triterpenoid synthase EhypOSC3 gene in preparation of cycloartenol

The invention relates to an euonymus japonicus triterpenoid synthase EhypOSC3 gene and application of the euonymus japonicus triterpenoid synthase EhypOSC3 gene in preparation of cycloartenol, and belongs to the technical field of biology. The nucleotide sequence of the euonymus japonicus triterpenoid synthase EhypOSC3 gene is as shown in SEQ ID NO.1, and the total length of the sequence is 2280bp; the amino acid sequence of the encoded protein is shown as SEQ ID NO.2, and 759 amino acid residues are encoded. The euonymus japonicus triterpenoid synthase EhypOSC3 gene disclosed by the invention can be used as a biosynthesis regulation gene of cycloartenol, is applied to preparation of cycloartenol, and is remarkable in application prospect and easy to popularize and apply.
Owner:YUNNAN AGRICULTURAL UNIVERSITY

Application of geum aleppicum total triterpenes in treating hypertension and preparation method of geum aleppicum total triterpenes

The invention discloses application of geum aleppicum total triterpenes in treatment of hypertension and a preparation method of the geum aleppicum total triterpenes, the geum aleppicum total triterpenes are obtained by step-by-step extraction and purification of geum aleppicum, and the geum aleppicum total triterpenes comprise bitter raspberry sweet F1, 2-hydroxyl oleanolic acid, 2-hydroxyl ursolic acid, 20beta, 20beta, 20beta, 20beta, 20beta, 20beta, 20beta, 20beta, 20beta The traditional Chinese medicine composition is prepared from the following raw materials in percentage by weight: 28-epoxy-28-hydroxytaxasan-3 beta-ol (taraxane triterpenes), ursolic acid, maslinic acid, rosa multiflora acid and tormentic acid. According to the present invention, the geum aleppicum total triterpenes are extracted and purified from the geum aleppicum step by step, and the geum aleppicum total triterpenes can significantly increase the total cross-sectional area of the vascular bed of the ischemic tissue or organ, such that the low blood pressure can meet the blood perfusion for maintaining the life activity so as to achieve the blood pressure lowering purpose; the compound can be widely applied to the field of hypertension prevention and treatment medicines.
Owner:HUANGSHAN CUILANFU BIOTECHNOLOGY CO LTD

Lucid ganoderma beer and preparation method thereof

The invention belongs to the field of beer fermentation, and particularly relates to a beer preparation method based on a lucid ganoderma extracting solution low-temperature adding process, which comprises the following steps: raw material preparation, saccharification, boiling and cooling, primary fermentation, lucid ganoderma concentrated solution preparation and secondary fermentation. A ganoderma lucidum concentrated solution is added in the secondary fermentation, specifically, after the primary fermentation is finished, sediments at the bottom of the fermentation barrel are abandoned, and upper-layer clear beer liquid is taken; bottling the clarified beer liquid, and adding sugar and the ganoderma lucidum concentrated extracting solution into a bottle; then sealing at 20-22 DEG C for secondary fermentation until the sugar is consumed completely; obtaining the lucid ganoderma beer. The ganoderma lucidum beer prepared by the method is rich in functional components such as ganoderma lucidum polysaccharides and triterpenes, and keeps the original refreshing and mellow taste of beer. CO2 generated by secondary fermentation is retained in the bottle, so that the product has fine and lasting foam and good taste.
Owner:ZHEJIANG SCI-TECH UNIV

A method for preparing a taiwanofungus camphoratus enzyme

The present application belongs to the field of biotechnology, and particularly relates to a preparation method of a taiwanofungus castaneus enzyme Trichoderma longibrachiatum and bacillus licheniformis Bacillus licheniformis are mixed to prepare a composite fermentation seed liquid. A culture medium composed of cassava residues and sawdust is used to prepare pretreated taiwanofungus castaneus culture medium. Then, the taiwanofungus castaneus strain is inoculated in the culture medium for fermentation, and a heat stress treatment step is introduced in the fermentation process. The method significantly promotes the synthesis and dissolution of active components of taiwanofungus castaneus triterpenoids and polysaccharides by the synergistic effect of specific strains and the combination of physical stress. The present application solves the technical problem of insufficient dissolution of active substances of taiwanofungus castaneus in the prior art by a unique strain combination and process design, and has high application value and market prospect.
Owner:湖南可诺耶生物科技有限公司

Novel triterpenoid glycoside compound extracted from rosa roxburghii tratt fruits as well as preparation method and application of novel triterpenoid glycoside compound

The invention relates to the technical field of medicines, in particular to a novel triterpenoid glycoside compound extracted from rosa roxburghii tratt fruits and a preparation method and application of the novel triterpenoid glycoside compound. The novel triterpenoid glycoside compound is Roxbuterene E, the molecular formula of the novel triterpenoid glycoside compound is C41H66O13, the novel triterpenoid glycoside compound is obtained by separating and purifying a rosa roxburghii tratt fruit methanol extract by adopting normal-phase silica gel column chromatography, reversed-phase ODS column chromatography and HPLC (High Performance Liquid Chromatography), and a PTP1B enzyme inhibitory activity experiment proves that the novel triterpenoid glycoside compound has potential activity for treating diabetes. And technical support is provided for research and development of novel diabetes treatment drugs.
Owner:GUIYANG COLLEGE OF TRADITIONAL CHINESE MEDICINE

A preparation method of an organic solvent nanofiltration membrane of a rigid-flexible dual network type IPN

A method for preparing an organic solvent nanofiltration membrane with a rigid-flexible dual-network IPN belongs to the field of membrane separation technology. The method includes: 1) dissolving aminated carbon quantum dots or triterpenoid amines containing a twisted structure and aromatic diacyl chlorides to obtain aqueous and oil-phase monomer solutions; using an ultrafiltration membrane as a support, the support is sequentially impregnated with the aqueous and oil-phase monomer solutions to construct a molecular layer with a porous rigid network I structure; 2) preparing organic solutions using an aliphatic amine as the first monomer and an aliphatic acyl chloride as the second monomer; immersing the support containing the porous rigid network I in the first monomer solution, then removing and washing it; then immersing the substrate in the second monomer solution, then removing and washing it, repeating this cycle a certain number of times, followed by heat treatment of the substrate to finally obtain the organic solvent nanofiltration membrane. The above preparation method produces a solvent-resistant nanofiltration membrane with high solvent flux, high separation accuracy, and strong long-term service stability.
Owner:ZHEJIANG UNIV

Glucoside hydrolase from intestinal bacteria and application thereof

The invention discloses a glycosyl hydrolase derived from intestinal bacteria and application of the glycosyl hydrolase. A glycosyl hydrolase gene for catalyzing trillioside to be hydrolyzed into diosgenin is cloned from intestinal microorganism enterococcus faecalis for the first time, and protein coded by the glycosyl hydrolase gene is identified as multifunctional glycosyl hydrolase; the diosgenin can be catalyzed to generate diosgenin, and the hydrolysis of other saponins such as steroid saponin and C3, C6 and C21 glycosyl of ginsenoside can be catalyzed, so that a basis is provided for the biological enzymolysis of the diosgenin and other saponins or sapogenin. Meanwhile, biological enzymolysis of the diosgenin makes up for the defects of high production cost, serious environmental pollution and the like of a traditional acid hydrolysis method, and the diosgenin has a great application prospect.
Owner:WUHAN BOTANICAL GARDEN CHINESE ACAD OF SCI

Sugar-controlling and weight-losing functional food cooperatively conditioned by endogenous enzymes of processing raw materials and preparation method thereof

The invention relates to a sugar-controlling and weight-losing functional food cooperatively conditioned by endogenous enzymes of processing raw materials and a preparation method thereof, and belongs to the technical field of food. The raw materials comprise 6-10 parts of white kidney beans, 4-8 parts of lucid ganoderma, 20-40 parts of black-skin and green-kernel black beans, 3-6 parts of mung beans and 40-60 parts of wheat flour / rice flour. A dual biological activation process is adopted: white kidney beans are subjected to low-temperature enzymolysis by yeast powder and probiotic soybean enzymes (1%, 25-35 DEG C) for 2-4 hours to release an alpha-amylase inhibitor, and ganoderma lucidum is subjected to weak-base hydrolysis by sodium bicarbonate (1-3%) for 2-4 hours to activate a triterpenoid glycosidase inhibitor; and performing compound fermentation on the zymolyte and the alkaline hydrolysis product for 2-4 hours, and then adding a cereal base material to activate the endogenous enzyme activity. High fiber combination (more than 15%) is used for prolonging satiety and reducing fat absorption, resistant starch (heat is only 50% of saccharides) is used for inhibiting lipid synthesis, and quadruple synergy (enzyme inhibition is more than 90% / resistant starch / low GI / high fiber) is combined, so that dual effects of postprandial blood sugar reduction and weight control are realized.
Owner:HANGZHOU GREEN SHANGJIA AGRICULTURAL SCIENCE & TECHNOLOGY SERVICE CO LTD

Ganoderma lucidum fermentation liquor as well as preparation method and application thereof

The invention relates to the technical field of yoghurt food, in particular to ganoderma lucidum fermentation liquor as well as a preparation method and application thereof. According to the preparation method provided by the invention, the preparation efficiency can be effectively improved, the content of ganoderan and triterpenoid components in the ganoderma lucidum fermentation liquor is remarkably increased, and large-scale production is facilitated. Metabolite produced in the preparation process of the ganoderma lucidum fermentation liquor can endow the yoghourt with unique flavor. The ganoderma lucidum fermentation liquor provided by the invention is combined with the yoghourt, so that the nutritional ingredients of the yoghourt can be enriched, and the nutritional value and the health-care function of the yoghourt can also be improved. According to the set yoghurt prepared by taking the ganoderma lucidum fermentation liquor and the milk as main raw materials, when the addition amount of the ganoderma lucidum fermentation liquor is 10wt% of that of the milk, the oxidation resistance, the acidity, the water-holding capacity and the sensory evaluation of the set yoghurt are optimal.
Owner:LIAOCHENG UNIV

Liposome adjuvant system based on furanose type QS-21 saponin molecule and preparation method

The invention provides a lipidosome adjuvant system based on furanose type QS-21 saponin molecules and a preparation method. The lipidosome adjuvant system comprises lipidosome and the QS-21 saponin molecules, the lipidosome comprises a lipid bilayer formed by ionizable lipid, structural phospholipid and cholesterol, the QS-21 saponin molecule is in a furanose type and is loaded on the lipidosome, and the QS-21 saponin molecule is partially inserted into the lipid bilayer through hydrophobic triterpenoid aglycone of the QS-21 saponin molecule. The preparation method comprises the following steps: dissolving lipidosome components forming lipid bilayers in an organic solvent to form a lipid phase, dissolving furanose type QS-21 saponin molecules in an acidic aqueous phase buffer solution to form a QS-21 aqueous phase, mixing the lipid phase and the QS-21 aqueous phase, precipitating lipid, and self-assembling to form lipidosome nanoparticles loaded with furanose type QS-21 saponin molecules, and finally, purifying to remove the organic solvent and the unencapsulated QS-21 molecules. According to the invention, through well-designed lipid composition and a preparation process, furanose type QS21 molecules are stably integrated into the lipidosome.
Owner:SUZHOU BAIFUXIN BIOPHARMACEUTICAL CO LTD +1

Method for simultaneously purifying triterpenoids, hawthorn flavonoids and hawthorn polysaccharides from hawthorn

The invention discloses a method for simultaneously purifying triterpenoids, hawthorn flavonoids and hawthorn polysaccharides from hawthorn, which comprises the following steps: crushing a hawthorn medicinal material, extracting, performing macroporous resin graded adsorption, performing gradient elution, performing membrane separation and the like to respectively obtain hawthorn extracts rich in triterpenoids, hawthorn flavonoids and hawthorn polysaccharides. According to the method, hawthorn is used as a raw material, the extraction, separation and purification method is simple, the used solvent is green, pollution-free and low in cost, hawthorn extracts rich in triterpenoids, hawthorn flavonoids and hawthorn polysaccharides are respectively obtained through macroporous resin graded adsorption, gradient elution, membrane separation and other methods, the effects of squeezing to be dry, taking all hawthorn flavonoids and comprehensively utilizing hawthorn flavonoids are achieved, the utilization rate is high, and the method is suitable for industrial production. The utilization value of hawthorn is improved, and the application prospect is wide.
Owner:劲牌持正堂药业有限公司 +1

Induction culture method for lavender suspension cells having high antioxidant activity

An induction medium and induction culture method for lavender suspension cells having high antioxidant activity. Suspension cells having a high ABTS free radical scavenging rate can be cultured by optimizing the formulation of a medium, and the contents of total phenols, total flavonoids and total triterpenoids are relatively high. Additionally, lavender suspension cells induced by using the medium grow rapidly and have a stable system, and extracts thereof can significantly reduce redundant ROS generated by oxidative stress of HaCaT cells, exhibit good biological antioxidant activity, and provide a reference for the development of lavender secondary metabolite resources and the like.
Owner:JIANGNAN UNIV

Microbial biocontrol agent, preparation method and application thereof

The present application provides a kind of microbial biocontrol agent and its preparation method and application, belong to the field of microorganism technology, the microbial biocontrol agent includes bacillus velezensis R_RT64 and bacillus licheniformis LX2501, not only can inhibit the growth of chain sporomycetes in ganoderma lucidum cultivation process, simultaneously can promote the increase of polysaccharide, triterpenoid, sterol, nucleoside and other beneficial active substances in ganoderma lucidum, greatly improve the quality of ganoderma lucidum, has broad application prospect in ganoderma lucidum cultivation field.
Owner:UNIV OF JINAN +1

Use of plantain fruit extract in preparation of anticoagulant or antithrombotic drugs

PendingCN122272702AHas anti-inflammatory propertiesHas antioxidant propertiesPlatelet inhibitionFlavonoid
This invention relates to the field of biomedicine, specifically to the application of banana fruit extract in the preparation of anticoagulant or antithrombotic drugs. The banana fruit extract provided by this invention contains flavonoids, polyphenols, polysaccharides, hydroxyanthraquinones, triterpenoids, and other components. Flavonoids and polyphenols have been widely proven to have anti-inflammatory and antioxidant effects, which can synergistically enhance the anticoagulant effect. The high potassium content of banana fruit can indirectly improve vascular function by regulating sodium-potassium balance. The banana fruit extract provided by this invention can inhibit platelet production or promote platelet metabolism, prolong clotting time, and inhibit platelet aggregation. Its natural plant components interfere with platelet aggregation by inhibiting the production of thromboxane A2, achieving anticoagulant and antithrombotic effects. The mechanism of action of banana fruit extract differs from traditional anticoagulants such as heparin, reducing the risk of bleeding. Its natural component characteristics provide a direction for the development of novel plant-derived anticoagulant drugs.
Owner:GUANGXI XIUPEI KELING BIOTECHNOLOGY CO LTD

Preparation device and preparation process of high-quality ganoderma triterpene sesame oil

The invention relates to the field of oil processing of oil plants, in particular to a high-quality ganoderma triterpenoid sesame oil preparation device and a preparation process thereof.The power assembly is arranged in the shell, the screening assembly is installed on the power assembly, and the cutting assembly is further installed on the power assembly and used for driving the cutting assembly to rotate; the power assembly drives the vibration assembly to vibrate, and the vibration assembly is used for periodically colliding with the screening assembly and the cutting assembly, so that the effect that materials clamped on the outer walls of the screening assembly and the cutting assembly are shaken off is achieved; the screening assembly and the cutting assembly convey materials to the pretreatment box through a pipeline, and are matched with the pretreatment box to achieve the effects of cleaning, drying, grinding and screening the materials; the invention solves the problem of how to improve the quality of the selected sesame and lucid ganoderma in the process of preparing the lucid ganoderma triterpenoid sesame oil.
Owner:ANHUI SCI & TECH UNIV +1

UHPLC Q-Exactive Orbitrap MS / MS (Ultra High Performance Liquid Chromatography Q-Exactive Orbitrap Mass Spectrometry / Mass Spectrometry) rapid identification method for chemical components and serum migration components of compound radix scutellariae capsule for clearing heat from throat

PendingCN120992792AComponent separationRapid identificationSerum pharmacochemistry
The invention discloses a UHPLC Q-Exactive Orbitrap MS / MS (Ultra High Performance Liquid Chromatography Q-Exactive Orbitrap Mass Spectrometry / Mass Spectrometry) rapid identification method for chemical components and serum migration components of a compound radix scutellariae capsule for clearing heat from throat, which is characterized in that the chemical components and the serum migration components of the compound radix scutellariae capsule for clearing heat from throat are rapidly qualitatively identified by adopting UHPLC Q-Exactive Orbitrap MS / MS; 21 in-vitro components (including flavonoids, phenolic acids, triterpenes and the like) and 21 in-blood migration components (including 9 in-blood prototype components and 12 metabolites) are identified. The chemical components and serum migration components of the compound radix scutellariae capsule for clearing heat from throat are defined, a foundation is laid for fundamental research of pharmacodynamic substances of the preparation, and the blank of chemical research of serum drugs of the compound radix scutellariae capsule for clearing heat from throat is filled up.
Owner:GUIZHOU RUIHE PHARMA CO LTD LONGLI PHARMA FACTORY

Heterocyclic triptycene mononuclear metal rhodium complex as well as preparation and application thereof

The invention belongs to the technical field of metal catalyst preparation, and provides a heterocyclic triptycene mononuclear metal rhodium complex and preparation and application thereof.The structural formula I of the heterocyclic triptycene mononuclear metal rhodium complex is shown in the specification, and the heterocyclic triptycene mononuclear metal rhodium complex has the advantages of being high in stability and activity and wide in substrate application range; and the compound has the advantages of simple synthesis steps, high yield and low cost, is suitable for various different metal catalytic reactions when being used as a catalyst, and has high popularization and application values.
Owner:ANHUI JINGSHENG LIGHT ENERGY TECHNOLOGY CO LTD

Triterpenoid alkaloid compound as well as preparation method and application thereof

The invention belongs to the field of traditional Chinese medicine extracts, and relates to a triterpenoid alkaloid compound as well as a preparation method and application thereof. The triterpenoid alkaloid compound is separated from euonymus japonicus for the first time, and it is proved that the triterpenoid alkaloid compound has remarkable anti-inflammatory activity and anti-tumor activity and has good application prospects in preparation of anti-inflammatory drugs and anti-tumor drugs. The preparation method comprises the following steps: taking dry euonymus japonicus as a raw material, sequentially carrying out heating reflux extraction for multiple times by using methanol, extracting an alkaloid-rich part by using an acid extraction and alkali precipitation method, carrying out preliminary segmentation by means of normal-phase silica gel column, amino silica gel column, gel column chromatography and the like, and carrying out refining purification by a preparative thin-layer chromatography, a semi-preparative high performance liquid chromatograph and the like. The preparation method is easily available in raw materials, environment-friendly, simple to operate and suitable for industrial production.
Owner:WUHAN CHILDRENS HOSPITAL

Research on extraction and purification process of Tibetan pawpaw total triterpenes

The invention belongs to the technical field of traditional Chinese medicine extraction and purification, and particularly relates to a Tibetan papaya total triterpenoid extraction and purification process which comprises the following five steps: degreasing, extraction, alcohol precipitation, column chromatography and drying. The research process is stable and feasible, the extraction efficiency is high, the purification effect is good, and scientific theoretical reference is provided for further development and utilization of the Tibetan papaya total triterpene part.
Owner:TIBET SHENHOU PHARM CO LTD

Ligustrum lucidum ait triterpene synthase LlucOSC1 gene and application thereof in preparation of cycloartenol

PendingCN120944925AFungiTransferasesEnzyme GeneLigustrum vulgare
The invention relates to a ligustrum lucidum ait triterpenoid synthase LlucOSC1 gene and application thereof in preparation of cycloartenol, and belongs to the technical field of biology. The nucleotide sequence of the ligustrum lucidum ait triterpene synthase LlucOSC1 gene is as shown in SEQ ID NO.1, and the full length of the sequence is 2283bp; the amino acid sequence of the encoded protein is shown as SEQ ID NO.2, and 760 amino acid residues are encoded. The ligustrum lucidum triterpene synthase LlucOSC1 gene can be used as a biosynthesis regulation gene of cycloartenol, is applied to preparation of cycloartenol, and is remarkable in application prospect and easy to popularize and apply.
Owner:YUNNAN AGRICULTURAL UNIVERSITY

Preparation method of intestinal targeting type high-absorption ganoderma lucidum composite fermented drink

The invention discloses a preparation method of an intestinal targeting type high-absorption ganoderma lucidum composite fermented beverage, and belongs to the technical field of functional food processing. The method comprises the following steps: separating supernate and mycelium from ganoderma lucidum fermentation liquor; ganoderma lucidum polysaccharides are extracted from mycelia and are subjected to enzymatic compounding with prebiotic oligosaccharides under the catalysis of transglycosidase to form a polysaccharide-prebiotic compound, and triterpenoids are loaded on the polysaccharide-prebiotic compound; taking the supernate and tea polyphenol to form a composite core, coating chitosan and citrus pectin through electrostatic layer-by-layer self-assembly, and constructing pH responsive nanoparticles; and finally, mixing the composite system, the nanoparticles and auxiliary materials, and sterilizing and filling to obtain the product. Through the synergistic effect of enzymatic compounding and nano assembly, targeted release and efficient absorption of active ingredients in intestinal tracts are achieved, bioavailability and product stability are remarkably improved, and the preparation method is suitable for preparing functional food for regulating intestinal flora and enhancing immunity.
Owner:ZHEJIANG FANGGE PHARMA

A pine wood nematode disease prevention and treatment agent and a preparation method thereof

The present application relates to nematode disease prevention and treatment agent technical field, especially to a kind of pine wood nematode disease prevention and treatment agent and preparation method thereof, by weight parts, including the following components: sophocarpidine 10-30 parts;Azadirachtin 5-20 parts;Turpentine 40-70 parts;Emulsifier 5-15 parts;Efficiency agent 1-5 parts;Stabilizer 0.5-3 parts, the synergistic effect of sophocarpidine and azadirachtin constitutes the core of the present application;Quinolone ring and pyridine ring structure in sophocarpidine molecule endow it with strong insecticidal activity, mainly by inhibiting acetylcholinesterase to interfere with the nerve conduction of nematode;And the complex triterpenoid structure of azadirachtin can interfere with the hormone system of nematode, inhibit its growth and development and reproduction.The two different mechanisms of action form complementation at molecular level, both can quickly kill nematode, and can long-term inhibit its population growth;Second, turpentine as natural terpene compound, the permeability of other active ingredients is enhanced.
Owner:HUNAN ACAD OF FORESTRY

Quality evaluation method and application of dandelion medicinal material and extract thereof

The invention discloses construction and application of a dandelion fingerprint spectrum and a multi-component quantitative analysis method. According to the method, the UHPLC fingerprint spectrum of the phenol components and the triterpenoid components of the dandelion is constructed for the first time, quantitative analysis of the six phenol components and the three triterpenoid components can be achieved at the same time, and the method can be used for comprehensive evaluation and quality control of dandelion raw materials and extracts of the dandelion raw materials.
Owner:劲牌持正堂药业有限公司 +1

Triterpenoid synthase EhypOSC2 gene of euphorbia lactuca and application thereof

The invention relates to an euphorbia lactuca triterpenoid synthase EhypOSC2 gene and application thereof, and belongs to the technical field of biology. The nucleotide sequence of the euonymus japonicus triterpenoid synthase EhypOSC2 gene is as shown in SEQ D NO.1, and the total length of the sequence is 2277 bp; the amino acid sequence of the encoded protein is shown as SEQ ID NO.2, and 758 amino acid residues are encoded. The euonymus japonicus triterpenoid synthase EhypOSC2 gene can be used as a biosynthesis regulation gene of beta-amyrin and lupeol and is applied to preparation of beta-amyrin, and the lupeol has a remarkable application prospect and is easy to popularize and apply.
Owner:YUNNAN AGRICULTURAL UNIVERSITY

Preparation method of ganoderma lucidum liupao tea

PendingCN122350195ABiotechnologyTriterpene
This invention discloses a method for preparing Ganoderma lucidum Liubao tea, belonging to the field of deep tea processing technology. Addressing the limitations of traditional Liubao tea's single fermentation method and limited functional components, this invention blends traditional Liubao tea raw materials with 5% Ganoderma lucidum ultrafine powder, undergoing a secondary light fermentation at 28-32℃ and 85% humidity for 7-10 days to form a stable microbial system. The final product is then fixed using a gradient drying technique. This method results in a finished product with both aged aroma and a sweet, mellow taste, increasing the theabrownin content by 15-20%, and enriching it with Ganoderma lucidum triterpenoid active ingredients. Breaking through traditional processes, this method achieves targeted transformation of tea's functional components, providing dual health benefits of immune regulation and antioxidant effects.

Process for preparing ganoderma lucidum extract

A process for preparing a low-plasticizer low-pesticide residue Ganoderma lucidum extract, comprising the steps of use of a Ganoderma lucidum fruiting body as a starting material, acid-water rinsing pretreatment, pulverization, composite enzyme coupling thermal extraction, filtration, column chromatography, use of an oxidant to remove plasticizer and pesticide residues, reverse osmosis membrane concentration, sterilization, spray drying, pulverization, sieving, granulation, etc. The starting material pretreatment and composite enzymatic hydrolysis coupling thermal extraction techniques can improve the leaching rate of Ganoderma lucidum triterpenoid and polysaccharide active ingredients. The active ingredient protection and application techniques during concentration can remove impurities to increase purity, eliminating the bitter taste of the Ganoderma lucidum extract. The oxidant can remove plasticizer and pesticide residues. The chromatography column adsorption and elution apparatus is optimized. The device is clamped by means of a clamping mechanism, and a snap-mounting mechanism assists in snap-mounting the device. In the process of subjecting the device to liquid storage by means of an adjustment apparatus, a driving apparatus is started to perform a closing adjustment on the adjustment apparatus, and the device is sealed by means of a sealing apparatus, so that the experimental convenience of the device and the efficiency of separation and purification can be improved.
Owner:ZHEJIANG SKYHERB BIOTECHNOLOGY INC

A fermentation method for improving the sleep-aiding function of old vinegar by using acetic acid stage enhanced pueraria-radish fermented powder in cooperation with zizyphus jujuba mill leaf powder

PendingCN122357245ABiotechnologyPuerarin
This invention belongs to the field of vinegar fermentation technology, and provides a fermentation method for enhancing the sleep-aiding function of aged vinegar by strengthening kudzu root-poria fermentation powder in the acetic acid stage in conjunction with jujube leaf powder. Kudzu root-Poria cocos fermentation powder is produced by fermenting kudzu root with superior Poria cocos strains. Based on the traditional solid-state fermentation process of Shanxi aged vinegar, kudzu root-Poria cocos fermentation powder and jujube leaf powder are added during the acetic acid fermentation stage. The acetic acid fermentation yields raw vinegar, which is then mixed with jujube seed extract, xylitol, and erythritol. The mixture is then sterilized at ultra-high temperature to obtain aged vinegar with sleep-aiding properties. The finished vinegar is reddish-brown in color, clear and transparent, with a rich and mellow vinegar aroma, a light herbal fragrance, a long aftertaste, and no other off-flavors. It contains γ-aminobutyric acid ≥35.00 mg / 100 mL, total flavonoids ≥250.00 mg / 100 mL, total saponins ≥20.00 mg / 100 mL, polysaccharides ≥350.00 mg / 100 mL, puerarin ≥6.50 mg / 100 mL, and triterpenoids ≥5.00 mg / 100 mL, and has sleep-aiding effects.
Owner:JINZHONG COMPREHENSIVE INSPECTION & TESTING CENT

Cultivation method of ganoderma lucidum spore powder for enhancing body immunity

The invention belongs to the technical field of artificial cultivation of medicinal fungi, and particularly relates to a ganoderma lucidum spore powder cultivation method capable of enhancing body immunity, which comprises the following steps: new strain cultivation, intelligent cultivation room construction cultivation, sterile cut-log matrix composite cultivation, directional spore collection and activity retention treatment. According to the invention, a molecular marker-assisted selection technology is adopted, strains with high content of beta-glucan and triterpenoids are accurately screened out from a wild ganoderma lucidum resource library, key gene markers related to synthesis of immune active components are determined through gene sequencing, and excellent strains are fused by using a protoplast fusion technology to cultivate a new strain. Therefore, the strain with high immune active component yield is quickly obtained, and the quality of the ganoderma lucidum spore powder and the effect of enhancing immunity are guaranteed from the source; by building the intelligent cultivation room, the most suitable environmental condition is provided for the growth of the lucid ganoderma, so that the growth of the lucid ganoderma and the synthesis and accumulation of immunocompetence components are promoted.
Owner:JIANGSU RONGZHITANG BIOMEDICAL TECH CO LTD