10 results about "Triterpenoid" patented technology

The present application belongs to the field of biotechnology, and particularly relates to a preparation method of a taiwanofungus castaneus enzyme Trichoderma longibrachiatum and bacillus licheniformis Bacillus licheniformis are mixed to prepare a composite fermentation seed liquid. A culture medium composed of cassava residues and sawdust is used to prepare pretreated taiwanofungus castaneus culture medium. Then, the taiwanofungus castaneus strain is inoculated in the culture medium for fermentation, and a heat stress treatment step is introduced in the fermentation process. The method significantly promotes the synthesis and dissolution of active components of taiwanofungus castaneus triterpenoids and polysaccharides by the synergistic effect of specific strains and the combination of physical stress. The present application solves the technical problem of insufficient dissolution of active substances of taiwanofungus castaneus in the prior art by a unique strain combination and process design, and has high application value and market prospect.

This invention relates to the field of biomedicine, specifically to the application of banana fruit extract in the preparation of anticoagulant or antithrombotic drugs. The banana fruit extract provided by this invention contains flavonoids, polyphenols, polysaccharides, hydroxyanthraquinones, triterpenoids, and other components. Flavonoids and polyphenols have been widely proven to have anti-inflammatory and antioxidant effects, which can synergistically enhance the anticoagulant effect. The high potassium content of banana fruit can indirectly improve vascular function by regulating sodium-potassium balance. The banana fruit extract provided by this invention can inhibit platelet production or promote platelet metabolism, prolong clotting time, and inhibit platelet aggregation. Its natural plant components interfere with platelet aggregation by inhibiting the production of thromboxane A2, achieving anticoagulant and antithrombotic effects. The mechanism of action of banana fruit extract differs from traditional anticoagulants such as heparin, reducing the risk of bleeding. Its natural component characteristics provide a direction for the development of novel plant-derived anticoagulant drugs.

This invention discloses a method for preparing Ganoderma lucidum Liubao tea, belonging to the field of deep tea processing technology. Addressing the limitations of traditional Liubao tea's single fermentation method and limited functional components, this invention blends traditional Liubao tea raw materials with 5% Ganoderma lucidum ultrafine powder, undergoing a secondary light fermentation at 28-32℃ and 85% humidity for 7-10 days to form a stable microbial system. The final product is then fixed using a gradient drying technique. This method results in a finished product with both aged aroma and a sweet, mellow taste, increasing the theabrownin content by 15-20%, and enriching it with Ganoderma lucidum triterpenoid active ingredients. Breaking through traditional processes, this method achieves targeted transformation of tea's functional components, providing dual health benefits of immune regulation and antioxidant effects.

This invention belongs to the field of vinegar fermentation technology, and provides a fermentation method for enhancing the sleep-aiding function of aged vinegar by strengthening kudzu root-poria fermentation powder in the acetic acid stage in conjunction with jujube leaf powder. Kudzu root-Poria cocos fermentation powder is produced by fermenting kudzu root with superior Poria cocos strains. Based on the traditional solid-state fermentation process of Shanxi aged vinegar, kudzu root-Poria cocos fermentation powder and jujube leaf powder are added during the acetic acid fermentation stage. The acetic acid fermentation yields raw vinegar, which is then mixed with jujube seed extract, xylitol, and erythritol. The mixture is then sterilized at ultra-high temperature to obtain aged vinegar with sleep-aiding properties. The finished vinegar is reddish-brown in color, clear and transparent, with a rich and mellow vinegar aroma, a light herbal fragrance, a long aftertaste, and no other off-flavors. It contains γ-aminobutyric acid ≥35.00 mg / 100 mL, total flavonoids ≥250.00 mg / 100 mL, total saponins ≥20.00 mg / 100 mL, polysaccharides ≥350.00 mg / 100 mL, puerarin ≥6.50 mg / 100 mL, and triterpenoids ≥5.00 mg / 100 mL, and has sleep-aiding effects.

This invention discloses the application of Ganoderma lucidum extract rich in triterpenoid ganoderic acids in the preparation of drugs for treating chronic obstructive pulmonary disease (COPD). This invention is the first to systematically evaluate the repair effects of Ganoderma lucidum extract on COPD damage using airway and alveolar organoid platforms. Mouse and human COPD lung organoid experiments demonstrated that the triterpenoid-rich Ganoderma lucidum extract significantly promoted ciliated cell regeneration, inhibited mucus hypersecretion, maintained basal stem cell homeostasis, inhibited inflammatory factor release, promoted epithelial cell proliferation, inhibited apoptosis, and promoted the regeneration of alveolar type II epithelial cells (AT2) in an alveolar organoid model. In vivo COPD mouse models confirmed that Ganoderma lucidum extract significantly improved survival rate, protected alveolar structural integrity, and reduced airway inflammation. LC-MS / MS analysis showed that it mainly contains triterpenoid components such as ganoderic acids A, B, C₂, C₆, D, H, G, and ganoderic acid A, and exerts its protective effect by regulating the AKT and Wnt signaling pathways. This invention provides new candidate drugs for the treatment of COPD and lays a solid experimental foundation for the development of lung injury repair drugs with independent intellectual property rights, showing broad prospects for clinical translation and industrial application.

The patent application belongs to the technical field of skin disease treatment, and particularly relates to an extraction method and device of total triterpenoids of Malanthera incana root and application, which comprises the following steps: S1, after the Malanthera incana root medicinal material is crushed, ethyl acetate is added for heating reflux extraction, and the extracted extract is filtered and concentrated to Malanthera incana root extract; S2, the Malanthera incana root extract after mixing is treated by adopting a silica gel wet method column packing and dry method sample loading technology, eluted by petroleum ether-ethyl acetate twice, and the petroleum ether-ethyl acetate eluent is collected; S3, the petroleum ether-ethyl acetate eluent is treated by vacuum concentration and vacuum drying, and the total triterpenoids of Malanthera incana root are obtained; the composition can produce more comprehensive treatment effect on the complex multifactor pathogenesis of vitiligo through the synergistic effect of multiple components and multiple targets.

This invention provides a CYP716 gene, protein, and applications for catalyzing the oxidation of β-amyrin at the C22α and C28 positions, belonging to the field of biotechnology. The CYP716 gene includes either CYP716BX1 (nucleotide sequence as shown in SEQ ID No. 1) or CYP716BX2 (nucleotide sequence as shown in SEQ ID No. 2). Studies have shown that CYP716BX1 and CYP716BX2 are isoenzymes, both capable of catalyzing the oxidation of β-amyrin at the C22α and C28 positions to 22α-hydroxy-kapogonadiol or 22α-hydroxy-oleanolic acid, and also capable of catalyzing the oxidation of 21β-hydroxy-β-amyrin at the C22α and C28 positions to 21β,22α-dihydroxy-kapogonadiol. This invention can provide key gene elements for the synthetic biology research of cyrimyl alcohol-type triterpenoids.

The application provides a method for simultaneously extracting polysaccharide and triterpenes in Poria cocos, and belongs to the technical field of biological extraction. The application uses specific first solvent and second solvent and water as solvents, increases the solubility of triterpenes in the upper phase, and makes the triterpenes enriched in the upper phase. Meanwhile, the alkali salt is used to dissolve the polysaccharide, so that the polysaccharide is enriched in the lower phase. In the process of extracting Poria cocos, the double water phase is formed, the triterpenes in the upper phase and the polysaccharide in the lower phase are simultaneously extracted and separated, the yield of the polysaccharide is close to 5%, the extraction rate is 79.9%, the yield of the triterpenes is 0.44% (4.4 mg / g), and the extraction rate is 63.7%. Moreover, the method of the application realizes the natural separation of the upper phase and the lower phase by using the solvent and the alkali salt, saves time, labor and materials, is simple to operate, and has a mild reaction, and has wide application value.