136 results about "Triterpenoid" patented technology

The invention belongs to the technical field of food microorganisms, and particularly discloses a ganoderma lucidum mutant strain capable of producing polysaccharides and triterpenoids at high yield. The strain is named as Ganoderma lucidum in Latin, the preservation number is CGMCC NO: 41680, the preservation unit is China General Microbiological Culture Collection Center, and the preservation date is December 4, 2024. The strain is named as Ganoderma lucidum, the preservation number is CGMCC NO: 41680, and the preservation number is CGMCC NO: 41680. The polysaccharide content in the ganoderma lucidum spore powder produced by the ganoderma lucidum mutant strain is more than or equal to 8%, and the total triterpenoid content is more than or equal to 12%; a culture medium formula of the ganoderma lucidum mutant strain comprises potato leachate, glucose and monopotassium phosphate, the culture temperature is 25-28 DEG C, and the pH value is 5.5-6.5. The ganoderma lucidum mutant strain is high in genetic stability, the content of active ingredients is remarkably higher than that of parent strains, and the ganoderma lucidum mutant strain is suitable for large-scale fermentation production.

The invention discloses a method for enhancing squalene synthesis through oil tea HMGR gene editing. According to the method, phosphorylation regulation and control sites Ser47 and / or Thr208 recognized by MAPK are targeted, and enzymatic activity inhibition is relieved and metabolic flux is enhanced by constructing phosphorylation resistance mutants (such as Ser-> Ala and Thr-> Val). The constructed editing vector can be introduced into camellia oleifera tissues in manners of PEG-mediated protoplast transient transfer, agrobacterium infection of calluses and the like, so that stable expression is realized. Experiments prove that the enzyme activity of HMGR in the editor is remarkably improved, the squalene accumulation amount is increased by 50% or above, the editing efficiency is high, expression is stable, and the method is suitable for high-value development of plant triterpenoid functional components and excellent strain breeding. The method also has the potential of popularization and application in other triterpenoid synthetic crops.

The invention provides a preparation method and application of lucid ganoderma nano powder, a lucid ganoderma polysaccharide extract or a lucid ganoderma triterpenoid extract, and belongs to the technical field of lucid ganoderma processing and preparation. The preparation method of the lucid ganoderma nano powder comprises the following steps: airing lucid ganoderma sporocarp, cutting into lucid ganoderma particles, and crushing the lucid ganoderma particles to obtain lucid ganoderma coarse powder; drying the ganoderma lucidum coarse powder at constant temperature, and performing superfine grinding on the dried ganoderma lucidum coarse powder to obtain the ganoderma lucidum powder. The ganoderma lucidum nano powder is successfully prepared through superfine grinding, the content of heavy metal in the ganoderma lucidum nano powder is low, the content of polysaccharide and the content of triterpenes measured in the ganoderma lucidum polysaccharide extract and the ganoderma lucidum triterpenes extract obtained through extraction of the ganoderma lucidum nano powder are high, and the antioxidant activity of the ganoderma lucidum polysaccharide extract and the antioxidant activity of the ganoderma lucidum triterpenes extract are high; further research finds that the ganoderma triterpenoid extract has remarkable activity of inhibiting brain glioma. Various functional products are prepared from the ganoderma lucidum nano powder, and the ganoderma lucidum nano powder has important significance and application value.

The invention relates to the technical field of daily chemicals, in particular to a soothing composition as well as a preparation method and application thereof. The composition is prepared from saccharomycetes fermentation lysate filtrate, peony root extract, spanishneedles herb extract, liquorice root extract, glycerol and tremella fuciformis extract. Small molecule peptides in the fermentation lysate and flavone, polyphenol and triterpenoid components in the plant extract can inhibit inflammation pathways (such as TNF-alpha, IL-6 and COX-2) in a multi-target mode, and skin redness is effectively relieved. The skin barrier repairing capability is high: the tremella acidic polysaccharide and the yeast polypeptide form a synergistic network, so that the water content of a cuticle is favorably enhanced, and barrier lipid synthesis is promoted; a polysaccharide-polypeptide cross-linked chain structure is broken through a high-shear homogenizing treatment technology, so that the wiredrawing and colloid decorous feeling of a finished product are remarkably reduced, and a refreshing skin feeling is obtained.

The invention relates to the technical field of cosmetics, in particular to an oil-controlling and acne-removing composition specially researched for teenagers and application of the oil-controlling and acne-removing composition. The fig fruit extract in the composition has oil-controlling, soothing and anti-inflammatory effects, the European fairweed bud extract can effectively reduce sebum secretion and inhibit expression of inflammatory factors, and the ganoderma lucidum extract is rich in triterpenoids and polysaccharide compounds and can effectively regulate skin health and enhance the barrier function of the skin, so that the skin can be effectively nourished, and the skin can be effectively nourished. The three substances are combined for use, so that the obvious oil-controlling and acne-removing effects are achieved.

The invention discloses a preparation method and application of a nano-composite for improving the bioavailability of a fat-soluble active substance curcumin by using a triterpenoid active micromolecule co-assembly. The invention aims to provide a preparation process of nanoparticles capable of loading active substances such as curcumin by utilizing the assembly characteristic of triterpenoid micromolecules and screening the combination proportion of materials. The preparation method is simple, convenient and low in cost. The nano-particles prepared by the method are spherical, uniform in particle size, and good in stability, water solubility and antioxidant activity; and the drug loading capacity of the nanoparticles encapsulated with the curcumin is 20.42%. According to the invention, high loading capacity of nutrient substances in the nano drug loading system is realized, and the bioavailability of polyphenol nutrient substances is improved.

The invention relates to the field of lucid ganoderma cultivation, and particularly discloses a method for increasing the content of ganoderma triterpenes in lucid ganoderma, which comprises the following steps: S1, preparing a culture medium; step S2, basswood manufacturing; step S3, inoculation; step S4, mycelium culture; step S5, burying soil and growing ganoderma lucidum; step S6, forming; from two aspects, firstly, a fermentation accelerant is added during preparation of the culture medium, and the fermentation accelerant is prepared by mixing calcium lactate, salicylic acid, a citrate-phosphate buffer solution and hydroxypropyl-beta-cyclodextrin according to a specific mass ratio; secondly, in the soil burying and ganoderma lucidum growing process, culture medium soil rich in fermentation extract is adopted for culture, the fermentation extract is prepared into a fermentation substrate through compound bacteria fermentation by taking Chinese wolfberry residues, liquorice residues and astragalus membranaceus residues as raw materials, and the content of ganoderma lucidum triterpenes in ganoderma lucidum is increased through the synergistic effect of all the substances.

The invention provides a high-efficiency fructus liquidambaris triterpenoid component extraction method, which comprises: S1, taking a fructus liquidambaris traditional Chinese medicine material, washing, airing, pulverizing with a pulverizer, and screening to obtain fructus liquidambaris test sample powder; s2, taking the fructus liquidambaris test sample powder, adding 80% ethanol, carrying out ultrasonic extraction for 0.5 h at 60 DEG C according to a solid-liquid ratio of 1: 10, and filtering to obtain a fructus liquidambaris triterpenoid component extracting solution; the invention also provides application of the extraction method in preparation of nitrite removal and anti-cancer active drugs. According to the method, the triterpenoid components in the liquidambar formosana hance are obtained by utilizing an ultrasonic extraction method, and optimal extraction parameters are obtained by combining a single-factor experiment with an orthogonal test response surface analysis method; based on the optimal extraction parameters, an efficient fructus liquidambaris triterpenoid component extraction method is obtained, and technical method guidance is provided for medical function development and application of fructus liquidambaris.

The invention belongs to the technical field of bioengineering, and particularly relates to an engineering bacterium for de novo biosynthesis of cycloastragenol as well as a construction method and application of the engineering bacterium. A mevalonic acid (MVA) pathway is reconstructed in a saccharomyces cerevisiae strain peroxidase body by utilizing a cell region compartment strategy, a key enzyme gene of the MVA pathway is over-expressed by using a strong promoter, and the cycloastragenol is obtained. The key enzyme genes are used as expression elements of cycloastragenol, are introduced into the chassis saccharomyces cerevisiae through a CRISPR-Cas9 technology, and are integrated on a saccharomyces cerevisiae genome through homologous recombination, so that a saccharomyces cerevisiae engineering bacterium CycZ23 and an engineering bacterium CAG-1 for efficiently synthesizing cycloastragenol are constructed, and de novo synthesis of plant triterpenoid natural products is realized; the highest titer of the generated cycloastragenol is 236 mg / L; the synthesis method is economical, efficient, green, environmentally friendly and easy to operate, and has important application value.

The invention discloses a composite capsule with synergistic stomach-nourishing and anti-inflammatory effects and a preparation method thereof, and relates to the technical field of functional foods, health care products and medicines. The lactic acid bacteria, the egg yolk globulin and the dandelion extract are used as main raw materials to prepare the composite capsule, the ratio of the raw materials is optimized, the three raw materials in the composite capsule generate a synergistic effect, triterpenoid components in the dandelion extract can inhibit helicobacter pylori, and the triterpenoid components, the lactic acid bacteria and the egg yolk globulin jointly repair gastrointestinal mucosa, so that the gastric mucosa is inhibited, and the gastric mucosa is prevented from being damaged. Gastrointestinal health is synergistically regulated, and resistance is enhanced; meanwhile, the dandelion extract and the egg yolk globulin serve as protective carriers, the survival rate of the lactic acid bacteria in gastric acid is increased, and experimental data shows that the survival rate is increased to 81% from 30%; compared with a single formula, the compound capsule can increase the survival rate of lactic acid bacteria and increase the bioavailability of the dandelion extract by enhancing the immune effect of egg yolk globulin.

The invention relates to an euonymus japonicus triterpenoid synthase EhypOSC3 gene and application of the euonymus japonicus triterpenoid synthase EhypOSC3 gene in preparation of cycloartenol, and belongs to the technical field of biology. The nucleotide sequence of the euonymus japonicus triterpenoid synthase EhypOSC3 gene is as shown in SEQ ID NO.1, and the total length of the sequence is 2280bp; the amino acid sequence of the encoded protein is shown as SEQ ID NO.2, and 759 amino acid residues are encoded. The euonymus japonicus triterpenoid synthase EhypOSC3 gene disclosed by the invention can be used as a biosynthesis regulation gene of cycloartenol, is applied to preparation of cycloartenol, and is remarkable in application prospect and easy to popularize and apply.

The invention discloses application of geum aleppicum total triterpenes in treatment of hypertension and a preparation method of the geum aleppicum total triterpenes, the geum aleppicum total triterpenes are obtained by step-by-step extraction and purification of geum aleppicum, and the geum aleppicum total triterpenes comprise bitter raspberry sweet F1, 2-hydroxyl oleanolic acid, 2-hydroxyl ursolic acid, 20beta, 20beta, 20beta, 20beta, 20beta, 20beta, 20beta, 20beta, 20beta The traditional Chinese medicine composition is prepared from the following raw materials in percentage by weight: 28-epoxy-28-hydroxytaxasan-3 beta-ol (taraxane triterpenes), ursolic acid, maslinic acid, rosa multiflora acid and tormentic acid. According to the present invention, the geum aleppicum total triterpenes are extracted and purified from the geum aleppicum step by step, and the geum aleppicum total triterpenes can significantly increase the total cross-sectional area of the vascular bed of the ischemic tissue or organ, such that the low blood pressure can meet the blood perfusion for maintaining the life activity so as to achieve the blood pressure lowering purpose; the compound can be widely applied to the field of hypertension prevention and treatment medicines.

The invention belongs to the field of beer fermentation, and particularly relates to a beer preparation method based on a lucid ganoderma extracting solution low-temperature adding process, which comprises the following steps: raw material preparation, saccharification, boiling and cooling, primary fermentation, lucid ganoderma concentrated solution preparation and secondary fermentation. A ganoderma lucidum concentrated solution is added in the secondary fermentation, specifically, after the primary fermentation is finished, sediments at the bottom of the fermentation barrel are abandoned, and upper-layer clear beer liquid is taken; bottling the clarified beer liquid, and adding sugar and the ganoderma lucidum concentrated extracting solution into a bottle; then sealing at 20-22 DEG C for secondary fermentation until the sugar is consumed completely; obtaining the lucid ganoderma beer. The ganoderma lucidum beer prepared by the method is rich in functional components such as ganoderma lucidum polysaccharides and triterpenes, and keeps the original refreshing and mellow taste of beer. CO2 generated by secondary fermentation is retained in the bottle, so that the product has fine and lasting foam and good taste.

The present application belongs to the field of biotechnology, and particularly relates to a preparation method of a taiwanofungus castaneus enzyme Trichoderma longibrachiatum and bacillus licheniformis Bacillus licheniformis are mixed to prepare a composite fermentation seed liquid. A culture medium composed of cassava residues and sawdust is used to prepare pretreated taiwanofungus castaneus culture medium. Then, the taiwanofungus castaneus strain is inoculated in the culture medium for fermentation, and a heat stress treatment step is introduced in the fermentation process. The method significantly promotes the synthesis and dissolution of active components of taiwanofungus castaneus triterpenoids and polysaccharides by the synergistic effect of specific strains and the combination of physical stress. The present application solves the technical problem of insufficient dissolution of active substances of taiwanofungus castaneus in the prior art by a unique strain combination and process design, and has high application value and market prospect.

The invention relates to the technical field of medicines, in particular to a novel triterpenoid glycoside compound extracted from rosa roxburghii tratt fruits and a preparation method and application of the novel triterpenoid glycoside compound. The novel triterpenoid glycoside compound is Roxbuterene E, the molecular formula of the novel triterpenoid glycoside compound is C41H66O13, the novel triterpenoid glycoside compound is obtained by separating and purifying a rosa roxburghii tratt fruit methanol extract by adopting normal-phase silica gel column chromatography, reversed-phase ODS column chromatography and HPLC (High Performance Liquid Chromatography), and a PTP1B enzyme inhibitory activity experiment proves that the novel triterpenoid glycoside compound has potential activity for treating diabetes. And technical support is provided for research and development of novel diabetes treatment drugs.

The present invention relates to a method for producing triterpenoids using one or more of the following polypeptides: (i) Saponaria officinalis synthase (SobAS), (ii) S. officinalis C28 oxidase (SoC28), (iii) S. officinalis C28C16 oxidase (SoC28C16), (iv) S. officinalis C23 oxidase (SoC23), (v) S. officinalis QA 3-O-glucuronosyltransferase (“SoCSL”), (vi) S. officinalis QA-GlcA galactosyltransferase (“SoC3Gal”), (vii) S. officinalis QA-GlcA-Gal xylosyltransferase (“SoC3Xyl”), (viii) S. officinalis QA-Tri fucosyltransferase (“SoC28Fu”), (ix) S. officinalis QA-TriF rhamnosyltransferase (“SoC28Rha”), (x) S. officinalis QA-TriFR xylosyltransferase (“SoC28Xyl1”), (xi) S. officinalis QA-TriFRX xylosyltransferase (“SoC28Xyl2”), (xii) S. officinalis QA-TriFRXX quinovosyltransferase (“SoGH1”), and (xiii) S. officinalis QA-TriF(Q)RXX acetyltransferase (“SoBAHD1”). Methods, host cells, isolated polypeptides, nucleic acids, and plants are provided.

A method for preparing an organic solvent nanofiltration membrane with a rigid-flexible dual-network IPN belongs to the field of membrane separation technology. The method includes: 1) dissolving aminated carbon quantum dots or triterpenoid amines containing a twisted structure and aromatic diacyl chlorides to obtain aqueous and oil-phase monomer solutions; using an ultrafiltration membrane as a support, the support is sequentially impregnated with the aqueous and oil-phase monomer solutions to construct a molecular layer with a porous rigid network I structure; 2) preparing organic solutions using an aliphatic amine as the first monomer and an aliphatic acyl chloride as the second monomer; immersing the support containing the porous rigid network I in the first monomer solution, then removing and washing it; then immersing the substrate in the second monomer solution, then removing and washing it, repeating this cycle a certain number of times, followed by heat treatment of the substrate to finally obtain the organic solvent nanofiltration membrane. The above preparation method produces a solvent-resistant nanofiltration membrane with high solvent flux, high separation accuracy, and strong long-term service stability.

The invention discloses a glycosyl hydrolase derived from intestinal bacteria and application of the glycosyl hydrolase. A glycosyl hydrolase gene for catalyzing trillioside to be hydrolyzed into diosgenin is cloned from intestinal microorganism enterococcus faecalis for the first time, and protein coded by the glycosyl hydrolase gene is identified as multifunctional glycosyl hydrolase; the diosgenin can be catalyzed to generate diosgenin, and the hydrolysis of other saponins such as steroid saponin and C3, C6 and C21 glycosyl of ginsenoside can be catalyzed, so that a basis is provided for the biological enzymolysis of the diosgenin and other saponins or sapogenin. Meanwhile, biological enzymolysis of the diosgenin makes up for the defects of high production cost, serious environmental pollution and the like of a traditional acid hydrolysis method, and the diosgenin has a great application prospect.

The present invention relates to methods, compositions, and combinations for inhibiting the NLRP3 inflammasome and / or LON protease as well as methods, compositions, and combinations for treating cancer, particularly blood cancer or brain cancer. In certain aspects, the compositions and combinations include a synthetic triterpenoid, such as 1-[2-Cyano-3,12-dioxooleana-1,9(11)-dien-28-oyl]-4(-pyridin-2-yl)-1H-imidazole (“CDDO-2P-Im”) and 1-[2-Cyano-3,12-dioxooleana-1,9(11)-dien-28-oyl]-4(-pyridin-3-yl)-1H-imidazole (“CDDO-3P-Im”).

To provide a conjugate for transferring an effector molecule from outside a cell into said cell.SOLUTION: Provided is a conjugate comprising an effector molecule, a cell surface molecule targeting antibody, and a saponin. Therein: the effector molecule, the antibody, and the saponin are covalently bound to each other; the effector molecule is an oligonucleotide; the saponin is a bi-desmosidic triterpene glycoside comprising an aglycone core structure selected from quillaic acid or gypsogenin and comprising a first glycan bound to a C3 atom of the aglycone core structure and a second glycan bound to a C28 atom of the aglycone core structure; the first glycan is branched and comprising a glucuronic acid unit; the second glycan is branched and comprising a carbohydrate unit selected from any one or more of fucose (Fuc), rhamnose (Rha), and quinovose (Qui); and the antibody is capable of binding to a cell surface molecule of the cell.SELECTED DRAWING: None

The invention relates to a sugar-controlling and weight-losing functional food cooperatively conditioned by endogenous enzymes of processing raw materials and a preparation method thereof, and belongs to the technical field of food. The raw materials comprise 6-10 parts of white kidney beans, 4-8 parts of lucid ganoderma, 20-40 parts of black-skin and green-kernel black beans, 3-6 parts of mung beans and 40-60 parts of wheat flour / rice flour. A dual biological activation process is adopted: white kidney beans are subjected to low-temperature enzymolysis by yeast powder and probiotic soybean enzymes (1%, 25-35 DEG C) for 2-4 hours to release an alpha-amylase inhibitor, and ganoderma lucidum is subjected to weak-base hydrolysis by sodium bicarbonate (1-3%) for 2-4 hours to activate a triterpenoid glycosidase inhibitor; and performing compound fermentation on the zymolyte and the alkaline hydrolysis product for 2-4 hours, and then adding a cereal base material to activate the endogenous enzyme activity. High fiber combination (more than 15%) is used for prolonging satiety and reducing fat absorption, resistant starch (heat is only 50% of saccharides) is used for inhibiting lipid synthesis, and quadruple synergy (enzyme inhibition is more than 90% / resistant starch / low GI / high fiber) is combined, so that dual effects of postprandial blood sugar reduction and weight control are realized.

The invention relates to the technical field of yoghurt food, in particular to ganoderma lucidum fermentation liquor as well as a preparation method and application thereof. According to the preparation method provided by the invention, the preparation efficiency can be effectively improved, the content of ganoderan and triterpenoid components in the ganoderma lucidum fermentation liquor is remarkably increased, and large-scale production is facilitated. Metabolite produced in the preparation process of the ganoderma lucidum fermentation liquor can endow the yoghourt with unique flavor. The ganoderma lucidum fermentation liquor provided by the invention is combined with the yoghourt, so that the nutritional ingredients of the yoghourt can be enriched, and the nutritional value and the health-care function of the yoghourt can also be improved. According to the set yoghurt prepared by taking the ganoderma lucidum fermentation liquor and the milk as main raw materials, when the addition amount of the ganoderma lucidum fermentation liquor is 10wt% of that of the milk, the oxidation resistance, the acidity, the water-holding capacity and the sensory evaluation of the set yoghurt are optimal.

The invention discloses an antihypertensive beverage formula and a preparation method thereof, and the antihypertensive beverage formula comprises the following components in parts by weight: 0.01-10 parts of saffron, 0.1-100 parts of sophora flower bud, 0.1-100 parts of huanglongcao, 0.1-100 parts of corn stigma, 0.1-100 parts of cassia occidentalis, 0.1-100 parts of white chrysanthemum and 0.1-100 parts of cistanche. According to the invention, the huanglongcao contains baicalin, baicalin, baicalein, scutellaria baicalensis enzyme and the like; the common selfheal fruit-spike contains triterpenoids, flavonoids, steroid glucoside and coumarin compounds, and the cassia seed contains anthraquinones and naphthopyrone compounds; when the components act in the human body, the CAMP (cyclic adenosine monophosphate) level of the human body is increased, so that the platelet accumulation is obviously reduced, the resistance of blood circulation is reduced, the pressure of blood on vascular walls is reduced, and the effects of reducing blood pressure, improving eyesight, inducing diuresis and inhibiting cholesterol synthesis are achieved, so that the purpose of reducing blood pressure is achieved.

The invention provides a detection method and a separation method for chemical components of Ainsliaea delavayi. According to the method, 38 compounds can be separated from the root of the Aleuristolochia delavayi by combining ultra-high performance liquid chromatography with a specific mobile phase and an elution program, 16 compounds can be rapidly identified from the root of the Aleuristolochia delavayi by further adopting an analysis method that the ultra-high performance liquid chromatography is connected with a quadrupole time-of-flight mass spectrometer in series, and a result shows that the method has the advantages that the method is simple and convenient to operate; the hedyotis diffusa roots are rich in ursane type triterpenes, oleanane type triterpenes and saponins thereof, and in addition, the hedyotis diffusa roots also comprise flavonoid, flavonoid glycoside and other components, so that the hedyotis diffusa roots have very strong ecological significance and relatively high development and utilization values. According to the invention, the chemical components of the hedyotis diffusa are enriched, a foundation is laid for research on pharmacological activity and development and utilization of the hedyotis diffusa, meanwhile, a novel preparation method of 16 compounds is provided, and the sources of the compounds are enriched.

The invention provides a lipidosome adjuvant system based on furanose type QS-21 saponin molecules and a preparation method. The lipidosome adjuvant system comprises lipidosome and the QS-21 saponin molecules, the lipidosome comprises a lipid bilayer formed by ionizable lipid, structural phospholipid and cholesterol, the QS-21 saponin molecule is in a furanose type and is loaded on the lipidosome, and the QS-21 saponin molecule is partially inserted into the lipid bilayer through hydrophobic triterpenoid aglycone of the QS-21 saponin molecule. The preparation method comprises the following steps: dissolving lipidosome components forming lipid bilayers in an organic solvent to form a lipid phase, dissolving furanose type QS-21 saponin molecules in an acidic aqueous phase buffer solution to form a QS-21 aqueous phase, mixing the lipid phase and the QS-21 aqueous phase, precipitating lipid, and self-assembling to form lipidosome nanoparticles loaded with furanose type QS-21 saponin molecules, and finally, purifying to remove the organic solvent and the unencapsulated QS-21 molecules. According to the invention, through well-designed lipid composition and a preparation process, furanose type QS21 molecules are stably integrated into the lipidosome.

The invention discloses a method for simultaneously purifying triterpenoids, hawthorn flavonoids and hawthorn polysaccharides from hawthorn, which comprises the following steps: crushing a hawthorn medicinal material, extracting, performing macroporous resin graded adsorption, performing gradient elution, performing membrane separation and the like to respectively obtain hawthorn extracts rich in triterpenoids, hawthorn flavonoids and hawthorn polysaccharides. According to the method, hawthorn is used as a raw material, the extraction, separation and purification method is simple, the used solvent is green, pollution-free and low in cost, hawthorn extracts rich in triterpenoids, hawthorn flavonoids and hawthorn polysaccharides are respectively obtained through macroporous resin graded adsorption, gradient elution, membrane separation and other methods, the effects of squeezing to be dry, taking all hawthorn flavonoids and comprehensively utilizing hawthorn flavonoids are achieved, the utilization rate is high, and the method is suitable for industrial production. The utilization value of hawthorn is improved, and the application prospect is wide.

An induction medium and induction culture method for lavender suspension cells having high antioxidant activity. Suspension cells having a high ABTS free radical scavenging rate can be cultured by optimizing the formulation of a medium, and the contents of total phenols, total flavonoids and total triterpenoids are relatively high. Additionally, lavender suspension cells induced by using the medium grow rapidly and have a stable system, and extracts thereof can significantly reduce redundant ROS generated by oxidative stress of HaCaT cells, exhibit good biological antioxidant activity, and provide a reference for the development of lavender secondary metabolite resources and the like.

The invention discloses a triterpenoid component cyclatraine A in stems and leaves of astragalus membranaceus and application of the triterpenoid component cyclatraine A in neuroprotection, and belongs to the field of medicines. According to the method, separation methods such as normal-phase silica gel column chromatography, reversed-phase ODS column chromatography and high-performance liquid chromatography are adopted, chemical components in the ethanol extract of the stems and leaves of astragalus membranaceus are separated and purified, and a new triterpenoid component is successfully found and identified. Meanwhile, through an A beta 25-35 induced damaged PC12 cell model, the protection effect of the new triterpenoid component on nerve cells is researched. Experimental results show that the new triterpenoid components in the stems and leaves of the astragalus membranaceus can remarkably protect PC12 cells from being damaged through an anti-inflammatory and apoptosis inhibiting mechanism. The discovery provides an important scientific basis for further development and utilization of the stems and leaves of astragalus membranaceus, and also provides a new research direction for research and development of neuroprotective drugs.

The present application provides a kind of microbial biocontrol agent and its preparation method and application, belong to the field of microorganism technology, the microbial biocontrol agent includes bacillus velezensis R_RT64 and bacillus licheniformis LX2501, not only can inhibit the growth of chain sporomycetes in ganoderma lucidum cultivation process, simultaneously can promote the increase of polysaccharide, triterpenoid, sterol, nucleoside and other beneficial active substances in ganoderma lucidum, greatly improve the quality of ganoderma lucidum, has broad application prospect in ganoderma lucidum cultivation field.

This invention relates to the field of biomedicine, specifically to the application of banana fruit extract in the preparation of anticoagulant or antithrombotic drugs. The banana fruit extract provided by this invention contains flavonoids, polyphenols, polysaccharides, hydroxyanthraquinones, triterpenoids, and other components. Flavonoids and polyphenols have been widely proven to have anti-inflammatory and antioxidant effects, which can synergistically enhance the anticoagulant effect. The high potassium content of banana fruit can indirectly improve vascular function by regulating sodium-potassium balance. The banana fruit extract provided by this invention can inhibit platelet production or promote platelet metabolism, prolong clotting time, and inhibit platelet aggregation. Its natural plant components interfere with platelet aggregation by inhibiting the production of thromboxane A2, achieving anticoagulant and antithrombotic effects. The mechanism of action of banana fruit extract differs from traditional anticoagulants such as heparin, reducing the risk of bleeding. Its natural component characteristics provide a direction for the development of novel plant-derived anticoagulant drugs.