1751 results about "Apoptosis" patented technology

The invention relates to a medical simulation experiment method and device for acting apoptosis vesicles on primary cartilage cells based on cell line culture, and the method mainly comprises the following steps: S1, co-culture system construction, S2, multi-dimensional observation and analysis, and S3, operation risk assessment. In the step S1, primary cartilage cells, primary immune cells and cell line culture apoptosis vesicles are co-cultured; s2, carrying out multi-dimensional observation and analysis on the influence of apoptosis vesicles on cells; and S3, mainly constructing a multi-dimensional risk scoring system, and carrying out risk grade division by applying a biological network model. The invention aims at simulating and evaluating the influence of cell line culture apoptosis vesicles on primary cartilage cells in vitro and the interaction between the cell line culture apoptosis vesicles and primary immune cells, so that an experimental basis is provided for subsequent clinical application.

The invention discloses a scylla paramamosain keep-alive and soft shell soft-keeping method, a low-temperature keep-alive mode refers to an on-ice keep-alive mode or a low-temperature acidification keep-alive mode, preferably, the low-temperature acidification keep-alive mode: cooling seawater to 16 DEG C or below by using a cooling-water machine, then adjusting the pH value of the seawater to be less than 8 to obtain low-temperature acidified seawater, and then cooling the low-temperature acidified seawater to obtain the scylla paramamosain soft shell soft-keeping method. And putting the soft-shell crabs which just molt into low-temperature acidified seawater for keeping alive and softness. The hardening of the soft shell can be obviously delayed by low-temperature acidified seawater and on-ice keep-alive; low-temperature acidified seawater and on-ice keep-alive soft-shell crabs can improve the delicate flavor and sweet taste of hepatopancreas of the soft-shell crabs, and the flavors of the two keep-alive soft-shell crabs are better than those of cold-stored dead soft-shell crabs; on-ice and low-temperature acidified seawater keep-alive interferes with glycolipid metabolism, oxidative stress and cell apoptosis of the soft-shell crabs, and compared with on-ice keep-alive, low-temperature acidified seawater keep-alive influences are milder than on-ice keep-alive.

The invention belongs to the field of biological medicines, and particularly discloses application of DNAJB6 in preparation of medicines for preventing or treating myocardial infarction (MI). In-vivo and in-vitro experiments find that the expression of DNAJB6 protein in an MI model is remarkably reduced, and the over-expression of DNAJB6 can improve the cardiac function of a mouse after MI, relieve myocardial fibrosis and inhibit hypoxia-induced myocardial apoptosis; and cell apoptosis is aggravated by low expression of DNAJB6. The overexpression of the DNAJB6 by using the myocardial specific adeno-associated virus (AAV) can be used as a new strategy for preventing and treating the MI, and an experimental basis is provided for developing a drug for targeting the DNAJB6.

The invention relates to the technical field of biological medicine, in particular to eye drops containing recombinant protein as well as a preparation method and application of the eye drops. Comprising the following components: 0.01%-1% of recombinant human TFF3 protein, 0.1%-1% of an osmotic pressure regulator, 0.08%-2% of a pH regulator, 0.05%-0.15% of a thickening agent, 0.01%-0.1% of a surfactant, 0.01%-0.1% of a protective agent and the balance of water for injection, the traditional Chinese medicine composition can be used for treating the xerophthalmia, can promote wound healing and cell migration, has the effects of inhibiting inflammation and cell apoptosis, and can act on a plurality of target spots at the same time, so that the tear film stability is improved, ocular surface inflammation is inhibited, the vicious circle of the xerophthalmia is broken, and the xerophthalmia is treated more comprehensively and effectively; the problems that in the prior art, xerophthalmia medicine targets are single, and xerophthalmia cannot be comprehensively and effectively treated are solved.

The invention belongs to the field of biological medicines, and discloses an application of Akkermansia muciniphila or an extracellular vesicle of the Akkermansia muciniphila in preparation of a medicine for alleviating the ischemic necrosis of the alcoholic femoral head. Based on the pathogenesis characteristics of the AIONFH, the Akk bacteria and the extracellular vesicles thereof are used for treating the AIONFH for the first time, the molecular mechanism of the Akk bacteria and the extracellular vesicles thereof for regulating and controlling the intestinal tract-skeleton axis function, inhibiting bone cell apoptosis and improving the bone trabecula microstructure is deeply researched, the anti-pressure ability of bone tissue is improved, and the research blank in the field is filled. Compared with a traditional treatment method, the osteonecrosis pathological change is improved through a single way, and a novel multi-target treatment strategy is provided from multiple aspects of regulating inflammatory response, recovering bone metabolism balance, promoting intraosseous blood supply and the like.

The invention belongs to the technical field of cytotoxicity intervention, and particularly relates to application of an iNOS inhibitor in preparation of a medicine for relieving cellular injury caused by toxin combined exposure. KK-1 cells serve as a research model, it is shown for the first time that MC-LR and NaNOS combined contamination can remarkably up-regulate the expression level of iNOS protein, and it is found through tests that Nos2 gene silencing can remarkably relieve tight junction damage and cell apoptosis caused by toxin combined exposure in the KK-1 cells; it is revealed for the first time that iNOS is a core target of toxin combined exposure induced cell tight junction damage and apoptosis, and the blank of toxic mechanism research in toxin combined exposure is filled. The application provides a new direction for a detoxification strategy of environmental toxin combined exposure, provides a theoretical basis for developing an iNOS-targeted inhibitor, and also provides a new target for etiological recognition, early diagnosis and prevention of related diseases caused by environmental toxin combined exposure.

The invention relates to the technical field of biological medicine, and particularly discloses a plant exovesicle combined preparation for treating non-small cell lung cancer and a preparation method and application thereof, and the plant exovesicle combined preparation comprises ginseng exovesicles and turmeric exovesicles. The plant external vesicle combined preparation provided by the invention is used for inhibiting non-small cell lung cancer cells and promoting apoptosis of the non-small cell lung cancer cells, and can be used for preparing medicines for treating the non-small cell lung cancer.

The invention relates to the field of microorganisms, and discloses lactobacillus rhamnosus and application thereof. The lactobacillus rhamnosus with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.33153, provided by the invention, has relatively strong reproductive capacity, can produce lactic acid and hydrogen peroxide at high yield, and has relatively strong antibacterial performance; the polypeptide has the potential of being applied to organisms; the composition can regulate and control inflammation and apoptosis related genes, so that the generation amount of inflammatory factors is reduced, cell growth and repair are promoted, and gene expression of aquaporin and collagen can be up-regulated, so that skin repair is promoted.

The present invention relates to: a temperature-sensitive hydrogel for cancer treatment; a photothermal composition comprising the hydrogel as an active ingredient; and a preparation method for the temperature-sensitive hydrogel for cancer treatment. The temperature-sensitive hydrogel of the present invention includes gold nanostars as active ingredients, and thus can generate heat by light irradiation, thereby exhibiting a photothermal therapy effect. In addition, the hydrogel temperature increases so that the release of nitric oxide (NO) from S-nitrosocysteine can be induced. In addition, the temperature-sensitive hydrogel of the present invention includes S-nitrosocysteine as an active ingredient so that, upon a temperature rise due to a photothermal reaction, the penetration of drugs into a tumor site can be improved by the release of NO, and at the same time, apoptosis of cancer cells can be directly caused; and includes an immunotherapy agent as an active ingredient so that tumor size can be effectively suppressed even with the passage of time. Therefore, as a material that enables complex treatment combined with photothermal therapy and immunotherapy, the temperature-sensitive hydrogel of the present invention having the aforementioned effect can be usefully utilized in the field of medicine for cancer treatment.

The invention discloses a composition with anti-hair loss and hair development efficacy, which is prepared from the following raw materials in weight percentage: 50-60% mulberry leaf essence, 10-20% lotus seed heart essence, 20-30% forsythia essence, 1-10% orientalis leaf essence, 10-20% green tea essence, and 1-10% lactobacillus fermentation liquid. The composition of the present invention effectively exerts a synergistic effect through different raw material action mechanisms, enhances VEGF and LEFI target expression, and achieves anti-hair loss and hair development through the following two approaches: first, regulating capillary growth and local blood circulation, providing nutrition for hair follicle growth; second, promoting the proliferation and differentiation of hair follicle cells, extending the hair follicle growth cycle, inhibiting hair follicle cell apoptosis, stimulating hair follicle growth factor and consolidating new and old hair follicles, thereby achieving the effect of anti-hair loss and hair development.

The invention discloses application of MY-1B in preparation of medicines for improving ovarian aging, belongs to the technical field of biological medicines, and aims to solve the problems of how to effectively delay ovarian aging and improve ovarian functions. Experiments prove that the mRNA m5C modification level of ovarian granular cells in the ovarian aging process is increased, and the expression level of methylated transferase Nsun2 is increased. Ovarian aging caused by mRNA m5C modification level increase can be improved by supplementing the MY-1B, which is embodied in that the MY-1B can reduce the mRNA m5C modification level of aging nest granular cells; the MY-1B can be used for effectively correcting the orbital cycle disorder of the aged ovary and delaying the decline of the ovarian function; the MY-1B plays an important role in reducing the apoptosis level of granulosa cells in senescent ovaries; the MY-1B can improve the storage capacity of ovaries and enhance the quality of oocytes, so that the fertilization rate and the embryonic development potential of the oocytes are improved.

The invention discloses a multi-dimensional efficacy evaluation system for colon cancer targeted drug screening, and relates to the technical field of medical assistance. Comprising a data acquisition module, an inference engine module and an output module. The data acquisition module acquires an organ sample response data set (including apoptosis rate A1, activity decline degree A2 and image structure change score B1) and a patient background data set (including gene mutation discrete variable set G and metabolic pathway continuous variable set M). The inference engine module executes dominant condition rules: generating a negative weight factor alpha according to a gene mutation state and a metabolic activation threshold value; generating a deduction item gamma based on a preset drug-resistant mutation subset; performing weighted calculation on the apoptosis rate and the activity decline degree to generate a basic drug effect score beta, and increasing beta through a critical value; and finally generating a comprehensive response score S. And the output module outputs a drug effect judgment result according to the S, and multi-dimensional accurate evaluation is realized.

The invention discloses a preparation method and application of a photo-thermal biosensor for tumor cell quantification and vitality dual-index liquid biopsy. Phosphatidylserine (PS) is exposed by enriching and cracking tumor cells, is identified and combined by an aptamer Apt2, and is driven to be separated from Apt2-Cu2-xTe NSs; after magnetic separation, separated supernatant is subjected to photo-thermal detection; the photo-thermal signal intensity is positively correlated with the number of cells, so that quantitative detection is realized; during cell apoptosis, PS is turned out of the membrane, Apt2-Cu2-xTe NSs is combined with the surface of the membrane, separated supernatant is subjected to photo-thermal detection after magnetic separation, and the photo-thermal signal enhancement amplitude is in negative correlation with cell activity. The platform has the advantages of POCT characteristics, low cost and portability, and a new tool is provided for precise tumor diagnosis and treatment.

The invention relates to application of periplocin in anti-lung adenocarcinoma drugs and a target screening method of periplocin. Periplocin can be used for preparing anti-cancer drugs for treating lung adenocarcinoma, and can inhibit proliferation, invasion and metastasis of lung adenocarcinoma cells and induce apoptosis of the lung adenocarcinoma cells. Meanwhile, the invention also provides a method for screening anti-lung adenocarcinoma related targets of periplocin by combining transcriptomics with network pharmacology, which comprises the following steps: firstly, searching effective active components and prediction action targets of periplocin through a TCMSP database, constructing network pharmacology to screen key components and targets, and then constructing and analyzing a regulatory network. Intersection genes are determined in combination with transcriptomics, and a new thought is provided for lung adenocarcinoma treatment and drug research and development.

The invention relates to the technical field of bioactive peptides, and discloses a walnut peptide with a neuroprotection function and a preparation method and application thereof, and predicts that a batch of small molecular walnut peptides have the neuroprotection function through enzymolysis of natural walnut protein, molecular weight screening and molecular docking. Experiments prove that the walnut peptide disclosed by the invention has a nerve protection function: the cell activity of nerve cells is improved, and the walnut peptide plays a role in preventing neurodegenerative diseases and other nerve-related diseases; the toxic effect of oxidative damage on nerve cells is relieved, nerve cell apoptosis is inhibited, the cell viability of the nerve cells is improved, and the function of treating nerve injury related diseases is achieved; the compound can be used for improving the activity of nerve cells, relieving oxidative damage of the nerve cells, adjusting the protein level of nerve damage related pathways, preparing related drugs for reducing blood pressure or resisting senile dementia or tumors and the like, and has a wide application prospect.

The invention discloses an effect of an Intestinia butyriproducens bacterium in anti-tumor immunotherapy, and finds that colonization of the Intestinia butyriproducens bacterium in intestinal tracts can inhibit growth of CT26 mouse subcutaneous transplanted tumors with poor response to the tumor immunotherapy, meanwhile, growth of human colorectal cancer cells is remarkably inhibited, and apoptosis of tumor cells is promoted for the first time. The application of the Intestinia butyriproducens bacterium in the anti-tumor immunotherapy has the advantages that the effect of the intestinia butyriproducens bacterium in the anti-tumor immunotherapy is achieved, and the application of the intestinia butyriproducens bacterium in the anti-tumor immunotherapy in the anti-tumor immunotherapy in the anti-tumor immunotherapy in the anti-tumor immunotherapy is achieved. Meanwhile, after colonization of the strain and combined administration of PD-1 monoclonal antibodies, the proportion of CD8 + T cells in mouse tumor infiltration lymphocytes is increased, the proportion of depleted PD-1 + CD8 + T cells is reduced, and the proportion of CD8 + T cells releasing functional cell factors interferon gamma and granzyme B is also increased, which shows that after colonization of the strain, the curative effect of tumor immunotherapy can be enhanced, and the tumor immunotherapy effect can be enhanced. The immunoregulation capability and the great clinical application value are realized.

The invention provides a lentivirus for treating craniopharyngeal tubuloma as well as a preparation method, application and a corresponding pharmaceutical composition of the lentivirus. The lentivirus carries an hTERT (human telomerase reverse transcriptase) promoter and a CDC6shRNA (complementary deoxyribonucleic acid) gene. According to the invention, an hTERT promoter and a CDC6shRNA gene sequence are inserted behind an enzyme cutting site of a vector, an obtained plasmid is transferred into an expression strain, the expression strain is cultured and screened to obtain a cloned strain, and the cloned strain is shaken and packaged to obtain the hTERT-CDC6shRNA lentivirus; when the lentivirus disclosed by the invention is inoculated into a tumor body of a patient with craniopharyngeal tubuloma, the result shows that the lentivirus can effectively inhibit proliferation of craniopharyngeal tubuloma cells and can induce self apoptosis of the craniopharyngeal tubuloma cells.

The invention relates to the technical field of biological medicine, in particular to a chronic disease treatment composition based on mesenchymal stem cells and a preparation method thereof, and the chronic disease treatment composition comprises the mesenchymal stem cells; the mesenchymal stem cells are adipose mesenchymal stem cells, dental pulp mesenchymal stem cells and bone marrow mesenchymal stem cells. According to the invention, the culture medium of the adipose-derived mesenchymal stem cells is improved, and the prepared adipose-derived mesenchymal stem cells have higher proliferation activity. Meanwhile, through experimental tests, the adipose-derived stem cells can inhibit proliferation of the prostatic hyperplasia epithelium BPH-1 cells and remarkably promote apoptosis of the prostatic hyperplasia epithelium BPH-1 cells, a new direction is provided for treatment of prostatic hyperplasia, and the adipose-derived stem cells have huge application value.

The invention discloses a traditional Chinese medicine composition for repairing and / or activating residual ovarian functions of an aging ovary, and a preparation method and application thereof, and relates to the technical field of biology. The traditional Chinese medicine composition is prepared from the following components: radix astragali, radix angelicae sinensis, radix ophiopogonis, radix polygonati officinalis, semen cuscutae, radix morindae officinalis, fructus rubi, rhizoma dioscoreae and folium mori. The invention further provides application of the traditional Chinese medicine composition in repairing and / or activating the residual ovarian function of the senescent ovary and improving the quality of the senescent oocyte, the traditional Chinese medicine composition can increase the volume and weight of the senescent ovary, increase the number of follicles of all levels in the senescent ovary, reduce apoptosis of granular cells in the ovary and improve the survival rate of the senescent ovary. The abnormal rate of oocytes, oxidative stress of the oocytes and DNA damage are reduced, development and maturation of residual follicles are promoted and maintained, and the traditional Chinese medicine composition has a wide application prospect.

The invention relates to the field of biological medicine, in particular to an apoptosis vesicle for treating acute kidney injury and a preparation method and application of the apoptosis vesicle, and the preparation method comprises the following steps: acquiring mesenchymal stem cells, subculturing to P3-P5, then inducing apoptosis, collecting supernate 12-20 hours later, and obtaining the apoptosis vesicle through differential centrifugation. Research finds that the mesenchymal stem cell-derived apoptosis vesicles can significantly improve acute kidney injury of mice induced by platinum antitumor drugs, have an immune regulation effect, and can reduce inflammation, promote anti-inflammatory response, relieve acute kidney injury, reduce cell apoptosis and promote cell proliferation, thereby repairing kidney injury. Compared with the existing drug MSC, the apoptosis vesicle has low immunogenicity, can be sterilized through filtration due to small size, is not complicated as cells in overall treatment and storage, is suitable for drug delivery, and provides more flexibility and accuracy for treatment.

The invention relates to a method for enhancing target cell removal capacity of immune cells and application. The method comprises the following steps: recombining CD58 and TP53 genes with a granzyme B gene so as to obtain recombinant genes NFAT-GZMB-CD58 and NFAT-GZMB-TP53, and meanwhile, adding a transcription factor NFAT binding site into a regulation region of the obtained recombinant genes. Then, the two recombinant genes are directionally inserted into specific gene loci of immune cells through a gene editing technology. Expression of the two recombinant genes is regulated and controlled by an immune cell activation signal pathway, and translation products of the two recombinant genes can be delivered to target cells of immune cells through a granzyme-perforin pathway, so that the scavenging capacity of the immune cells to the target cells is enhanced, and apoptosis and escape of the target cells are inhibited. The method can be applied to the field of cell therapy.

The invention belongs to the technical field of functional grease processing, and particularly relates to an enzymolysis preparation method and application of diglyceride with an effect of improving Alzheimer's disease. The method comprises the following steps: mixing low-erucic acid rapeseed oil, glycerol and water, and heating to obtain a pre-reaction mixture; adding compound enzyme for reaction, and then adding candida antarctica lipase B and pseudomonas fluorescens lipase for reaction; and after the reaction is completed, carrying out molecular distillation, collecting a light-phase product, carrying out adsorption filtration, and collecting filtrate to obtain the diglyceride oil. The compound enzyme is composed of rhizomucor miehei lipase, candida rugosa lipase and candida antarctica lipase B in a weight ratio of (2-4): 2: 1. According to the method, the content of diglyceride is effectively increased, the prepared composition has the efficacy of preventing and treating Alzheimer's disease, and the efficacy is shown as improvement of reaction capacity and cognitive function, inhibition of brain apoptosis and acetylcholin esterase, enhancement of SOD and CAT vitality and improvement of related gene expression.

The present invention relates to combined therapy of cancer using an epithelial cell adhesion molecule (EpCAM) inhibitor and a Wnt signaling inhibitor. Specifically, the EpCAM inhibitor is an antibody which is directed to an extracellular domain (EpEX) of EpCAM. The combined therapy is effective in inducing apoptosis of cancer cells, inhibiting cancer sternness properties, tumor progression and / or metastasis, and / or prolonging survival of a cancer patient.

The invention discloses an AuAgPt ternary alloy nano-framework, a preparation method thereof and application of the AuAgPt ternary alloy nano-framework in anti-tumor preparations, and belongs to the field of biomedical nano-materials. The AuAgPt ternary alloy nano frame is a hollow octahedral nano frame formed by 12 AuAgPt ternary alloy edges, and the nano frame has plasmon photothermal conversion performance, catalase activity and peroxidase-like activity, and can be used for preparing the nano frame. The aptamer has the characteristics that the aptamer can form a covalent bond with glutathione (GSH), the aptamer with the framework surface capable of being modified and specifically bound with tumor cells is used for tumor targeted delivery and the like, the aptamer can be bound in a targeted manner and internalized into the tumor cells, and after near-infrared laser irradiation, the cell temperature can be increased to 42 DEG C, reactive oxygen species (ROS) can be generated, GSH can be consumed, and the tumor targeting effect is achieved. In addition, the content change of small molecule metabolites in cells can be driven through a low-temperature photothermal effect, the metabolic state is disturbed, the redox balance in the cells is destroyed, the tumor cells are induced to generate ferroptosis and apoptosis, and the low-temperature photothermal treatment of tumors is realized.

The invention belongs to the technical field of biological medicine, and particularly relates to application of hUC-MSCs combined with Met in preparation of anti-diabetic drugs. The diabetes mellitus is particularly type 2 diabetes mellitus. Through construction of a T2DM rat model, paraffin tissue section detection, serum ELISA, cell experiments and the like, researches prove that T2DM can be effectively treated by HUC-MSCs combined with Met treatment, including reduction of body blood sugar and recovery of blood sugar regulation ability; liver injury caused by T2DM is relieved and repaired; the glycolipid metabolism of the liver of the T2DM rat is improved; the immune inflammation disorder caused by T2DM is relieved; and the apoptosis level of liver cells is improved. And compared with a single hUC-MSCs or Met treatment mode, the combined treatment mode has a better treatment effect. The invention provides a theoretical basis and a new treatment strategy for treatment of T2DM.

The invention discloses application of an azetidine compound in preparation of a medicine for preventing and treating triple-negative breast cancer, and belongs to the technical field of biological medicine. By adopting an international common triple-negative breast cancer modeling method, the scheme shows that the compound can effectively inhibit the tumor growth state of nude mouse triple-negative breast cancer, inhibit STAT3 705 site tyrosine phosphorylation and induce tumor cells to generate G2-M phase arrest and apoptosis, and has remarkable prevention and treatment effects on triple-negative breast cancer; the compound is expected to become a novel medicine for treating triple-negative breast cancer.

The invention relates to the technical field of biological medicines, and provides a self-assembled polypeptide-rhein conjugate, a preparation as well as a preparation method and application of the self-assembled polypeptide-rhein conjugate. The sequence of the MMP-9 responsive self-assembled polypeptide-rhein conjugate is Rhein-GFFPLGLAGACE, and the MMP-9 responsive self-assembled polypeptide-rhein conjugate can respond to abnormally activated MMP-9 in atherosclerotic plaque and is self-assembled into a stable nanofiber structure in situ at a diseased region; the anti-inflammatory and anti-oxidation functions of rhein in the conjugate are remarkable, but the water solubility and oral bioavailability are low, the anti-inflammatory and anti-oxidation functions are remarkably improved after being coupled with the polypeptide, targeted delivery is achieved, and the residence time is prolonged; the introduction of the tripeptide ACE simulating the function of glutathione further enhances the oxidative stress resistance of the conjugate. Furthermore, the conjugate shows excellent anti-inflammatory and anti-oxidation effects, can clear ROS, inhibit inflammation pathways, regulate macrophage phenotypes and inhibit apoptosis, and provides a new strategy for treatment of atherosclerosis.

The invention belongs to the technical field of nano-drugs, and discloses a multifunctional nano-drug delivery system for treating neovascular eye diseases and a preparation method of the multifunctional nano-drug delivery system. The nano drug delivery system disclosed by the invention is a composite nano preparation which takes dendritic mesoporous silica nanoparticles as a carrier and co-loads cerium zirconium oxide nano enzyme and an anti-VEGF (vascular endothelial growth factor) drug, various active oxygen can be efficiently removed, oxygen is continuously released through catalytic decomposition of hydrogen peroxide, a retina hypoxia microenvironment is improved, and the retina hypoxia effect is improved. According to the present invention, the anti-VEGF drug delivery system can reduce the oxidative stress induced retinal pigment epithelial cell apoptosis rate, reduce the inflammatory reaction, and simultaneously achieve the slow release and the controlled release of the anti-VEGF drug, and the drug effect maintenance time can achieve more than 60 days, such that the multi-target synergistic treatment can be achieved through the synergistic regulation of the multiple pathological links such as oxygen deficit-oxidative stress-inflammation-angiogenesis, and the anti-VEGF drug delivery effect can be achieved. The choroidal neovascularization can be effectively inhibited by single intravitreal injection, and a new strategy of multi-target collaborative treatment is provided for neovascular macular degeneration and other eye diseases.

The invention discloses an effectiveness exploration method for inhibiting AM by exciting LIPUS through a flexible ultrasonic patch, and belongs to the field of medical equipment and biomedical engineering. The patch comprises a flexible substrate, an island bridge type metal electrode, an adjustable array type piezoelectric ceramic unit and a flexible packaging layer, can be precisely attached to a focus area, outputs low-intensity pulse ultrasound with the frequency of 1-3 MHz and the sound intensity of 30-120 mW / cm < 2 >, and can inhibit expression of proinflammatory factors, up-regulate E-cadherin, induce apoptosis of ectopic endometrial cells and inhibit activity of pain ion channels, so that the pain of a patient is relieved, and the pain of the patient is relieved. In-vivo and in-vitro experiments show that the patch does not have significant uterus and ovarian toxicity, can reduce the fibrosis area by 40% or more, can increase the apoptosis rate to 45%, supports a continuous or pulse type adjustable treatment mode, integrates a flexible electronic technology and ultrasonic medicine, and has a wide application prospect. A noninvasive, accurate and wearable innovative treatment scheme is provided for adenomyosis, and the clinical transformation value is remarkable.

The invention discloses a light-driven / temperature-driven double-trigger shape memory antibacterial and anti-tumor bone scaffold and a fused deposition modeling (FDM) preparation method thereof, and is characterized in that polylactic acid (PLA) is used as a matrix, and polyethylene glycol (PEG) and nano magnesium hydroxide (Mg (OH) 2) / copper oxide (CuO) are uniformly dispersed through an organic solvent blending method; a composite wire is prepared through the processes of drying and film forming, crushing and granulation and screw extrusion, and finally, the integrated molding of the three-dimensional porous scaffold is realized through an FDM (fused deposition modeling) process. According to the stent, temperature and near-infrared light double-triggered shape memory is achieved through cooperation of glass transition regulation and control of PLA-PEG and the photothermal effect of the nano metal oxide particles. Nano Mg (OH) 2 / CuO endows the scaffold with multi-mode antibacterial and anti-tumor functions: 1) Mg < 2 + > / Cu < 2 + > releases and destroys microbial membrane potential; according to the stent, the limitation of shape fixation of a traditional implant is broken through, shape self-adaption can be triggered through light / heat stimulation, irregular bone defects can be accurately attached, the risk of a secondary operation is avoided, and the stent has remarkable advantages in minimally invasive orthopedic repair.