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165 results about "Heat shock" patented technology

The heat shock response (HSR) is a cellular response that increases the number of molecular chaperones to combat the negative effects on proteins caused by stressors such as increased temperatures, oxidative stress, and heavy metals. In a normal cell, protein homeostasis (proteostasis) must be maintained because proteins are the main functional units of the cell. Proteins take on a defined configuration in order to gain functionality. If these structures are altered, critical processes could be affected, leading to cell damage or death. With the importance of proteins established, the heat shock response can be employed under stress to induce heat shock proteins (HSP), also known as molecular chaperones, that help prevent or reverse protein misfolding and provide an environment for proper folding.

A heat shock flash evaporation loop flow ascending heat exchange test system and test method

The present application relates to a kind of heat shock flash evaporation loop lift membrane heat exchange test system, including heat exchange pipe, vapor-liquid separator, condenser, metering barrel, vacuum pump, liquid tank, heat shock heater, throttle valve, measurement and control system and high-speed camera, the upper outlet of heat exchange pipe is connected with the import of vapor-liquid separator, the secondary steam outlet of vapor-liquid separator is connected with the hot medium side import of condenser, the hot medium side outlet of condenser is connected with metering barrel and liquid tank respectively, the concentrated liquid outlet of vapor-liquid separator is connected with liquid tank, the outlet of metering barrel is connected with liquid tank, vacuum pump is connected with liquid tank, liquid tank is connected with heat shock heater, heat shock heater is connected with heat exchange pipe, throttle valve is arranged between heat shock heater and heat exchange pipe;High-speed camera is arranged in one side of heat exchange pipe.The present application can realize the experimental test to heat shock flash evaporation loop lift membrane heat exchange technology, and provide support for mechanism research, equipment optimization of heat shock flash evaporation loop lift membrane heat exchange technology.
Owner:TIANJIN LEKE ENERGY SAVING TECH CO LTD +1

A heat pump rectification system based on heat shock flash circulation lifting heat exchange

The present application relates to a kind of heat pump rectification systems based on heat shock flash circulation lift heat exchange, including rectifying tower, lift membrane reboiler, first throttling valve and heat shock heater, the secondary steam outlet of rectifying tower is connected with the heating steam import of lift membrane reboiler, the discharge port of the bottom of rectifying tower is connected with the cold side flow channel import of heat shock heater, and the cold side flow channel export of heat shock heater is connected with the feed inlet of lift membrane reboiler by first throttling valve;The vapor-liquid mixture outlet of lift membrane reboiler is connected with the column still liquid reflux port of rectifying tower, and the steam condensate outlet of lift membrane reboiler is connected with condensate tank, and the liquid outlet of condensate tank is connected with the condensate reflux port of rectifying tower side wall.The present application is combined by heat shock pressurization energy storage and throttling flash kinetic energy release, provides independent and stable lift membrane kinetic energy for lift membrane heat exchange, so that the flow pattern in the tube of lift membrane reboiler directly enters the efficient annular flow stage, improves the industrial applicability of lift membrane heat exchange technology in heat pump rectification system.
Owner:TIANJIN LEKE ENERGY SAVING TECH CO LTD +1

A heat shock flash evaporation loop lift heat exchanger

The present application relates to a kind of hot exciting flash evaporation loop lift heat exchanger, including upper head, lower head, cylinder, several heat exchange tubes, the upper end surface of cylinder and the upper head form cavity I between, the lower end surface of cylinder and the lower head form cavity II between, cavity I, cavity II and the inner cavity of cylinder are not communicated with each other between, heat exchange tube is fixed in the inside of cylinder by tube sheet, the upper end of heat exchange tube is placed in cavity I and is communicated with cavity I, lower end and cavity II are communicated, cylinder includes lift section cylinder and hot exciting section cylinder, heat exchange tube includes lift section heat exchange tube and hot exciting section heat exchange tube, lift section heat exchange tube and hot exciting section heat exchange tube are communicated by throttling component between, the inner diameter of throttling component is less than the inner diameter of hot exciting section heat exchange tube.The heat exchanger of the present application is provided with a kind of hot exciting flash evaporation loop lift, will hot exciting pressurization energy storage, throttling flash evaporation kinetic energy release, loop lift heat exchange and vapor-liquid separation function integration in same equipment, solve the problem of traditional lift section heat exchanger dependence big temperature difference.
Owner:TIANJIN LEKE ENERGY SAVING TECH CO LTD +1

Microwave and steam medical waste continuous treatment process

The invention relates to the technical field of medical waste treatment, in particular to a microwave and steam medical waste continuous treatment process which comprises the following process steps: S1, conveying: conveying medical wastes from a feeding system into a crushing system through an elevator for crushing; s2, crushing, wherein the crushed medical waste is conveyed to a sterilization system through a conveying system for sterilization treatment; s3, sterilization is conducted, specifically, when the medical waste reaches a sterilization system, high-temperature and high-pressure steam is introduced into a sterilization bin; s4, exhausting: starting a circulating air pump to pump out harmful gas in the sterilization bin for purification; and S5, after inspection and sterilization are completed, the medical waste is discharged out of the sterilization bin through a discharging pipe. According to the medical waste sterilization device, the interior of the sterilization bin can be kept in a high-temperature and high-pressure state, so that bacteria in medical waste are sterilized, the equipment stability is good, damage caused by cold and heat shock is avoided, and meanwhile the problems of extremely low treatment capacity, high energy consumption, large leakage amount and the like caused by previous intermittent treatment are solved.
Owner:HEBEI HONGKE QINGNENG ENVIRONMENTAL PROTECTION EQUIP CO LTD

System and method for testing dynamic permeability of hydrate rock core produced by pressure reduction or heat shock method

The invention discloses a dynamic permeability testing system and method for hydrate core production by a pressure reduction or heat shock method, and the system comprises a dynamic permeability testing unit (1) which is used for the preparation of a natural gas hydrate core sample and the measurement of permeability; the gas and liquid injection unit (2) is used for injecting required gas and liquid into the dynamic permeability testing system; the hydraulic oil control unit (3) is used for applying axial pressure and confining pressure to the dynamic permeability testing system; the gas-liquid separation metering unit (4) is used for metering the gas-liquid flow at the outlet; and the data acquisition monitoring and control unit (5) is used for monitoring data of each unit and controlling each unit to work. According to the invention, the natural gas hydrate-containing rock core can be subjected to pressure drop under the triaxial stress condition or the production dynamic permeability test by a heat shock method.
Owner:SOUTHWEST PETROLEUM UNIV

Steam condensate water waste heat recovery and utilization device

The application provides a steam condensate waste heat recycling device, and relates to the field of heat energy recovery.The steam condensate waste heat recycling device comprises a base, a heat preservation cylinder arranged on the base, and a synchronous adjusting mechanism arranged on the heat preservation cylinder.The steam condensate waste heat recycling device can conveniently collect condensate water into a required device, and the high-temperature condensate water can not lose a large amount of heat through a pipeline and a surface of equipment during a transmission process, so that heat energy is not wasted.The synchronous adjusting mechanism drives a conveying pump to quickly transfer the condensate water in the heat preservation cylinder to a required place, so that the condensate water recovery efficiency is improved.The synchronous adjusting mechanism solves the problem of waste of condensate water waste heat in the prior art.The synchronous adjusting mechanism solves the problem of equipment damage caused by heat shock in the prior art.The synchronous adjusting mechanism solves the problem of increased thermal resistance caused by scale accumulation in the prior art.
Owner:PERSSON ENVIRONMENTAL PROTECTION TECH CO LTD

Method for preparing high-entropy spinel / biochar composite material by Joule thermal shock method and application of high-entropy spinel / biochar composite material

The invention belongs to the technical field of nano material synthesis and volatile organic compounds (VOCs) degradation, and particularly relates to a method for preparing a high-entropy spinel / biochar composite material by using a Joule thermal shock method and application of the high-entropy spinel / biochar composite material. The preparation method comprises the following steps: firstly, forming gel from metal salt precursors and biomass through a sol-gel method, and then rapidly synthesizing the precursors into the high-entropy spinel by adopting Joule thermal shock. The biochar in the composite material prepared by the invention is of a wormlike lamellar structure, and the high-entropy spinel is polygonal particles and is uniformly loaded on the biochar. Due to the high-entropy effect and lattice distortion effect of the composite material, the number of oxygen vacancies of the material can be obviously increased, defect sites can be formed on the surface of the material through instant energy impact, the catalytic performance of the material can be improved, and the catalytic material can efficiently convert methylbenzene into carbon dioxide and water.
Owner:JIANGSU NAO NEW MATERIAL CO LTD

Device and method for testing thermal shock resistance and thermal erosion resistance of material

The invention discloses a device and a method for testing thermal shock resistance and thermal erosion resistance of a material. The box body is used for providing a closed test chamber; the sample testing platform and the laser head are arranged in the testing chamber, the sample testing platform comprises a rotating shaft and a rotating testing table for loading a testing sample, and the rotating testing table is coaxially and fixedly connected to the rotating shaft; the laser head is arranged above the rotary test table and is connected with a main body of a laser generating device outside the box body, and the laser generating device carries a powder feeding system for feeding out test powder; the rotary driving mechanism is used for driving the rotating shaft to rotate, so that the laser emitted by the laser head periodically acts on a test sample on the rotary test table; and the cooling mechanism is used for cooling the rotary test bench. According to the testing device and the testing method provided by the invention, the working environment of thermal shock and thermal erosion of the material in long-term production can be truly restored and simulated, so that the thermal shock resistance and thermal erosion resistance of the material are tested.
Owner:SICHUAN UNIV

Double-report plasmid system and construction method and application thereof

The invention discloses a double-report plasmid system and a construction method and application thereof. The double-report plasmid system comprises a translation report plasmid and an error folding response plasmid; the translation reporter plasmid comprises a target protein gene sequence, an mRNA secondary structure sequence and a luciferase gene sequence; the misfolding response plasmid comprises a heat shock pressure promoter and a fluorescent protein gene. The double-report plasmid system can quantitatively and visually detect translation characteristics and wrong folding conditions of the recombinant protein, the whole operation process is carried out based on a 96-well plate, the double-report plasmid system has the characteristics of low cost, high timeliness, high flux and the like, and the recombinant protein can be rapidly and efficiently soluble (positive: complete soluble; negative: completely insoluble; and in an intermediate state, the solubility is relatively poor), so that the efficiency of recombinant protein expression research is greatly improved.
Owner:SUZHOU INST OF NANO TECH & NANO BIONICS CHINESE ACEDEMY OF SCI

Method for mass production of all-female triploid mandarin fish

PendingCN122319994AHeat shockAnimal science
This invention discloses a method for large-scale breeding of all-female triploid mandarin fish, comprising the following steps: (1) Preparation of all-female diploid fertilized eggs: using male mandarin fish pseudo-males (XX) induced by androgens and female mandarin fish (XX) selected from superior strains as parent fish, collecting sperm and eggs, mixing the two, adding water at a temperature of 28℃ to activate sperm for fertilization, and obtaining all-female diploid fertilized eggs; (2) Heat shock induction of triploids: after the all-female diploid fertilized eggs are fertilized in the above-mentioned water at a temperature of 28℃ for 8 minutes, they are immediately transferred to water at a temperature of 39~41℃ for 1~2 minutes for heat shock induction, and all-female triploid fertilized eggs are obtained; (3) Seedling hatching and cultivation: the fertilized eggs induced by heat shock in step (2) are transferred to the hatching system for hatching, and then conventional seedling cultivation is carried out to obtain all-female triploid mandarin fish seedlings. The mandarin fish fry produced by this method have a high female rate, triploid rate, and survival rate.
Owner:SUN YAT SEN UNIV

Therapeutic laser system and method of use for activating the tissue stem cell niche for the treatment of medical conditions

A system for treating various medical conditions in humans includes a medical or therapeutic laser operating at wavelengths in the near infrared range of between 1250 nm-1267 nm, with a target wavelength of 1260 nm, at power levels between 2 Watts and 60 Watts, and for a period not to exceed 60 seconds per dose. The emitted laser light energizes, e.g., the stem cell niche or mitochondria of the human tissue to which it is applied, both directly and indirectly via activation of cell signaling intermediaries including but not limited to heat shock proteins, thereby stimulating cell activity to promote healing and reduce pain. In this specific wavelength range, water absorption of the laser light energy is plateaued which effectively acts as a conduit allowing the laser light energy to be transferred deeper into the tissue without causing thermal damage to the skin and tissue.
Owner:KAIROS LASERS LLC

A method for cultivating konjak

The application discloses a kind of cultivation methods of konjak, belong to konjak planting technical field, the present application is to the pretreatment konjak seed potato of sterilization in nitrogen and ozone mixed atmosphere steam treatment, not only strengthen the growth potential of konjak seed potato, and fully activated the catalase in seed potato, create excellent intracellular environment for promoting subsequent seed potato germination, heat shock seed potato is soaked in activation liquid and activated, brassinolide, calcium sulfate, ethiofencarb and sodium complex nitrophenol in activation liquid activate the cytokinin oxidase in cell, and then regulate seed potato germination speed and germination quality, effectively solve the problem of low seedling rate of offspring konjak and yield decline.The activation seed potato is sowed in nutrient soil, effectively improves the content of nutrient substance in harvested konjak.
Owner:SHAANXI ANKANG LIYUSHAN ECOLOGICAL AGRICULTURE TECHNOLOGY CO LTD

Thermal shock resistance testing device for heat-shrinkable tape or sleeve

The utility model relates to a thermal shock resistance testing device for a thermal contraction belt or sleeve, which comprises a support, a shaft rod, a pressing sheet and a sample strip, two ends of the shaft rod are rotatably arranged at the top of the support through bearing seats, the sample strip is tightly pressed by the pressing sheet and is tightly attached to the outer circle of the shaft rod, the pressing sheet is fixed with the shaft rod through a screw, and a handle is eccentrically arranged at one end of the shaft rod. According to the utility model, the test process is simplified, the sample strip is hung on the rotatable shaft rod, and the sample strip is bent while whether the sample strip flows or drops is observed, so that the crack test of the sample strip is facilitated, the test efficiency is greatly improved, and the device is suitable for batch test.
Owner:JIANG SU CHENG XIN JIAN YAN JIAN CE REN ZHENG GU FEN YOU XIAN GONG SI

A method for efficiently inducing heteropolyploid fry

ActiveCN118947633BHeat shockAnimal science
This invention discloses a method for efficiently inducing allopolyploid fish fry, belonging to the field of fish breeding technology. The method of this invention includes the following technical steps: (1) Induction of spawning in distant hybridization parents: Selecting female and male parent fish from different subfamilies of Cyprinidae, and collecting mature eggs and semen respectively; (2) Obtaining hybrid fertilized eggs: Mixing the collected eggs with semen, and performing dry artificial insemination to obtain hybrid fertilized eggs; (3) Inducing allopolyploidy: Placing the fertilized eggs in a water bath for heat shock treatment to inhibit the expulsion of the second polar body or inhibit the first cleavage, and then quickly transferring the treated fertilized eggs to aquaculture water to continue hatching fry. The method of this invention combines the method of distant hybridization between subfamilies with the method of heat shock induction. After induction using this method, the multiplication rate of hatched fry can reach 100%, which significantly improves the yield of allopolyploid fish fry from distant hybridization.
Owner:HUNAN NORMAL UNIVERSITY

Squid salad processing method based on gradient temperature curing

PendingCN122320170AHeat shockIce water
This invention relates to the field of aquatic product deep processing technology, and discloses a squid salad processing method based on gradient temperature cooking. The squid salad is made from cooked squid rings, mixed vegetables, and a salad dressing containing a specific compound emulsifying stabilizer. The processing method includes: subjecting pre-treated squid to acoustic-thermal coupled gradient cooking, sequentially undergoing three stages: ultrasonic-assisted low-temperature sol-gelation, silent medium-temperature heat-induced gelation, and high-temperature safe setting; subsequently, immediately immersing it in an ice-water mixture for gradient heat-blocking cooling, draining, and then mixing with the dressing. This invention effectively solves the problems of severe moisture loss and tough texture in traditional processes by precisely controlling the timing of ultrasonic intervention and three-stage temperature control, combined with the heat shock effect, giving the product a crispy exterior and tender interior texture. Simultaneously, the use of compound stabilizers improves the emulsification stability and water-holding capacity of the dressing in a high-humidity matrix, increasing the yield of the finished product.
Owner:MARINE FISHERIES RES INST OF ZHEJIANG +2

Low-temperature constant-temperature cold source using latent heat of adsorption

The application discloses a low-temperature constant-temperature cold source utilizing adsorption latent heat, which is provided with an adsorption container filled with an adsorbent and filled with a working gas, and utilizes the adsorption latent heat generated when the working gas is desorbed to provide the cold source. The low-temperature constant-temperature cold source utilizes the adsorption latent heat generated when the working gas is desorbed to provide the cold source for a cooling platform. The application does not have any active moving parts, and has the characteristic of no vibration; the adsorption of the working gas on the surface of the adsorbent can occur in a wide temperature range higher than the boiling point of the working gas, so that the cooling can be provided in a wide temperature range; the utilization of the adsorption latent heat as the cold source can cope with greater heat shock, has better temperature stability, and has the advantages of simple device structure, easy-to-obtain material, convenient operation, low cost and the like.
Owner:SHANGHAI HYMASTER TECH CO LTD

Heating control device, heating control method and program

To provide a heating control device, a heating control method and a program enabling energy saving while suppressing heat shock to a user.SOLUTION: A cloud server 1 includes: a hot water filling start detection section 2111 that detects start of hot water filling; a temperature acquisition section 2114 that acquires indoor temperature information indicating a temperature of at least one of a bathroom and an undressing room and a room temperature information indicating a temperature of a room of a user when the start of hot water filling is detected; a heating necessity determination section 2115 that determines the necessity of heating at least one of the bathroom and the undressing room on the basis of the indoor temperature information and the room temperature information; and a control information generation section 116 that generates control information for controlling at least one of a bathroom heating device installed in the bathroom to heat the bathroom and an undressing room heating device installed in the undressing room to heat the undressing room when the heating necessity determination section determines the necessity of heating at least one of the bathroom and the undressing room.SELECTED DRAWING: Figure 16
Owner:MITSUBISHI ELECTRIC CORP

Compositions and Methods for Germinating Wild-Type or Super-Dormant Bacillus Strains

A germinant composition for activating one or more wild-type and / or super-dormant spores, particularly Bacillus spores, comprises L-isoleucine or L-valine or both. A germinant composition may also comprise a buffer, a source of potassium, an industrial preservative, and one or more other L-amino acids. Wild-type and / or super-dormant spores may be incubated with a germinant composition by heating to an incubation temperature of 26 to 60° C. for an incubation period of 30 minutes to 36 hours. Incubation may be a single step process without pre-activation or heat shock or a two-step process. A method of pre-activation comprises combining spores with a germinant composition and either bioprocessing or heat treating and then drying. An incubated bacteria solution preferably comprises at least 50% activated spores after an incubation period of 24 hours and more preferably after 1 hour.
Owner:NCH CORP

Experimental device for detecting thermal shock performance of protective coating

The utility model discloses an experimental device for detecting the thermal shock performance of a protective coating. The experimental device comprises an experimental table, a cooling water tank, a motor, a turntable, a connecting rod and a flame spray gun, the experiment table comprises a frame body, a table plate and a supporting plate; an avoiding hole is formed in the table plate, the bottom end of the cooling water tank is fixed to the supporting plate, and the top end of the cooling water tank upwards penetrates out of the avoiding hole and is provided with a cooling water filling opening; the motor is fixed on the bedplate; the turntable is positioned above the cooling water filling port and is in transmission connection with an output shaft of the motor; the connecting rods are radially arranged along the turntable, first ends of the connecting rods are fixed on the turntable, clamps are fixed at second ends of the connecting rods, and the clamps can clamp a to-be-tested metal piece coated with a protective coating; the flame spraying gun is installed on the platen, and the flame spraying end of the flame spraying gun faces the rotating disc. The experimental device for detecting the thermal shock performance of the protective coating disclosed by the utility model is time-saving and labor-saving, and a metal piece to be detected coated with the protective coating can be rapidly and repeatedly heated and cooled.
Owner:Liupanshan Laboratory

A blue copper-containing peptide-containing composition for repairing photothermal damage and application thereof

This invention relates to the field of cosmetics, specifically to a composition containing copper peptides for repairing photothermal damage and its application. The composition comprises a complex polypeptide, galactosomal yeast fermentation product filtrate, and cyanobacterial extract; wherein the complex polypeptide includes copper peptides, yeast polypeptides, and hexapeptide-11. This invention, by combining specific complex polypeptides with galactosomal yeast fermentation product filtrate and cyanobacterial extract, increases the expression level of heat shock proteins, thereby achieving excellent efficacy in repairing photothermal damage. Furthermore, this composition is mild and non-irritating, and can be widely used in cosmetics.
Owner:GUANGDONG BAIWEN BIOLOGICAL TECH CO LTD

Reagents, compositions and methods for improving viability and function of cells, tissues and organs

Compounds, compositions and methods for improving the viability and / or function of cells or for the in vitro, ex vivo or in vivo protection of cells, tissue, graft or organs from various damages are described. The reagents and composition are based on activation of the heat shock response activation and / or the antioxidant response and include for example. HSP90 co-factor inhibitor such as Celastrol or Celastrol analogs used alone or in combination with an adjunct agent (e.g., a NRF-2 activator, antioxidant, etc.). Therapeutic enhancement may also include increase in paracrine effector production and signaling. Methods for improving the resistance of cells, tissue, grafts or organs to damages or stress, such as hypoxic or oxidative stress-induced cell death, and / or for improving the viability and retention of transplanted or transfused cells are also described. Therapeutic treatment or prevention of ischemic injury (e.g. myocardial infarct, ischemia / reperfusion injury) and related stressors (hypoxia, oxidative stress, inflammation, sepsis / shock, etc) are also provided.
Owner:TARGA BIOMEDICAL INC

Application of ZmGOLS2 gene and ZmRAFS gene in improving pollen viability under heat shock and drought conditions of corn

The application discloses application of ZmGOLS2 genes and ZmRAFS genes in improving pollen vitality of corn under heat shock and drought conditions, and belongs to the technical field of genetic engineering. The application improves the pollen vitality of corn, promotes the germination of pollen under heat shock and drought stress conditions, and reduces the empty stalk rate of corn under drought stress conditions by simultaneously improving the expression levels of a corn myo-inositol galactoside synthetase coding gene and a corn raffinose synthetase coding gene.
Owner:NORTHWEST A & F UNIV

A system adapted to stimulate the activation of heat shock proteins using ultrasonic energy.

A method is provided for applying a pulsed energy source to living tissue to stimulate the activation of heat shock proteins and promote protein repair without damaging the tissue. A method for thermally treating biological tissue includes providing a pulsed energy source having energy parameters selected to raise the target temperature to a level that achieves a therapeutic effect, while maintaining the increase in average temperature of the tissue over time below a predetermined level so as not to permanently damage the target tissue. Application of the pulsed energy source to the target tissue induces a heat shock response and stimulates the activation of heat shock proteins in the target tissue to therapeutically treat the target tissue.
Owner:OJAI RETINAL TECHNOLOGY LLC

Preparation method of high-protein high-fiber zero-fat bionic eyefish maw

The invention provides a preparation method of a high-protein high-fiber zero-fat bionic eye fish maw, which comprises the following steps: mixing pea protein isolate, bone gelatin and water, adding glutamine transaminase and a compound flavor enzyme preparation, reacting at 50-60 DEG C for 30-60 minutes, then adding spirulina powder, and uniformly dispersing to obtain a protein base material; mixing and swelling konjaku flour, apple pectin and warm water, adding kappa-carrageenan, ultramicro kelp powder, calcium chloride and sodium carbonate, and homogenizing at 55-65 DEG C to form a gel matrix, so as to obtain a fiber base material; the method comprises the following steps: feeding a protein base material and a fiber base material through two independent feeding ports of a twin-screw extruder, and co-extruding through a die head with a plurality of layers of alternate runners to form a composite blank with alternate protein layers and fiber layers; and carrying out directional stretching and calcification, steam heat shock and flavor solidification, and cooling and shaping to obtain the bionic eye fish maw. By adopting the technical scheme, the protein content is more than or equal to 60% (dry basis), the dietary fiber content is more than or equal to 15%, and the fat content is less than or equal to 0.5%.
Owner:HUBEI ACCORD JIAXIAN BIOTECHNOLOGY CO LTD

Membrane-coated oncolytic virus as well as preparation method and application thereof

PendingCN121450596AOrganic active ingredientsVirusesPancreas Ductal AdenocarcinomaIntrahepatic Cholangiocarcinoma
The invention belongs to the technical field of biological medicines, and particularly relates to a membrane-coated oncolytic virus as well as a preparation method and application thereof. The oncolytic virus contains a GSDMD N-terminal expression cassette regulated and controlled by a heated shock promoter, and pyroptosis can be induced under exogenous mild thermal stimulation, so that the virus is promoted to be quickly released, and reinfection and amplification of adjacent tumor cells are realized. A bionic nano-vesicle (iNV) formed by a donor cell membrane modified by genetic engineering is used as an outer layer to coat a genetically engineered oncolytic virus (GOV) to obtain a membrane-coated oncolytic virus iNV-GOV, so that the membrane-coated oncolytic virus iNV-GOV simultaneously has immunocompatibility and active tumor recognition capability, is suitable for solid tumors such as pancreatic ductal adenocarcinoma (PDAC), hepatocellular carcinoma (HCC), intrahepatic cholangiocarcinoma (IHCC) and the like, and can be used for preparing an anti-tumor drug. And the tumor volume can be obviously reduced in mouse in-vivo model experiments.
Owner:HANGZHOU RUIDAO GENE TECH CO LTD

Reagents, compositions and methods for improving viability and function of cells, tissues and organs

Compounds, compositions and methods for improving the viability and / or function of cells or for the in vitro, ex vivo or in vivo protection of cells, tissue, graft or organs from various damages are described. The reagents and composition are based on activation of the heat shock response and / or the antioxidant response and include for example, HSP90 co-factor inhibitor such as Celastrol or Celastrol analogs used alone or in combination with an adjunct agent (e.g., a NRF-2 activator, antioxidant, etc.). Therapeutic enhancement may also include increase in paracrine effector production and signaling. Methods for improving the resistance of cells, tissue, grafts or organs to damages or stress, such as hypoxic or oxidative stress-induced cell death, and / or for improving the viability and retention of transplanted or transfused cells are also described. Therapeutic treatment or prevention of ischemic injury (e.g. myocardial infarct, ischemia / reperfusion injury) and related stressors (hypoxia, oxidative stress, inflammation, sepsis / shock, etc) are also provided.
Owner:TARGA BIOMEDICAL

A therapeutic agent that is a heat shock protein (HSP) inhibitor and its use in cancer treatment

The invention relates to the use of the compound ES3707 as a therapeutic agent for the treatment of cancer diseases and for alleviating symptoms caused by such diseases, specifically by inhibiting heat shock proteins involved in cell division and by preventing resistance induced by clinical drugs. The therapeutic agent subject to the invention is characterized by its enhanced efficacy when used in combination with photodynamic therapy.
Owner:BOZOK ÜNİVERSİTESİ ÖZEL KALEM REKTÖRLÜK +3

Preparation method of high-purity TIR2 and T1R3 recombinant plasmids

The invention discloses a preparation method of high-purity TIR2 and T1R3 recombinant plasmids. The preparation method comprises the following steps: transforming the T1R2 and T1R3 recombinant plasmids into modified Trans1-T1 escherichia coli competent cells through a heat shock method; large-scale amplification of plasmids is carried out by utilizing the efficient cloning characteristic of Trans1-T1 escherichia coli; and extracting and purifying recombinant plasmids from thalli by adopting an optimized alkali cracking combined column purification technology. According to the preparation method of the high-purity TIR2 and T1R3 recombinant plasmids, target DNA fragments or recombinant DNA plasmids constructed by the target DNA fragments are introduced into competent cells, amplification of genetic materials is achieved through a replication and expression system of host cells, finally the high-purity DNA plasmids are obtained through extraction and purification, and the high-purity TIR2 and T1R3 recombinant plasmids are obtained by improving transformation, amplification and purification processes. The yield and the purity of the T1R2 / T1R3 recombinant plasmid are remarkably improved, and the high-standard requirement of a transfection experiment is met.
Owner:CHINA TOBACCO HENAN IND CO LTD

Thermal shock resistant plate-fin heat exchanger

The utility model relates to the technical field of fin heat exchangers, and discloses a thermal shock resistant plate-fin heat exchanger which comprises a heat exchanger body, the inlet end of the heat exchanger body is fixedly communicated with a hot medium inlet pipe and a cold medium inlet pipe respectively, and the outlet end of the heat exchanger body is fixedly communicated with a hot medium outlet pipe and a cold medium outlet pipe respectively. Buffer pipes are arranged on one side of the hot medium inlet pipe and one side of the cold medium inlet pipe, hemispherical sealing blocks are fixedly connected into the buffer pipes, first springs are fixedly connected to the outer surface of the bottom of a connecting disc, and a spherical blocking block is fixedly connected to the outer surface of the bottom of a blocking rod. The spherical blocking block is impacted by fluid, so that the first spring is compressed, the inflow hole is communicated, the first spring buffers the strong impact force of the fluid, the impact force of the impact force of the fluid on the plate bundle in the heat exchanger is reduced, the impact resistance is strong, and the service life of the heat exchanger is prolonged.
Owner:WUXI MASHAN YONGHONG HEAT EXCHANGER