71 results about "Heat shock" patented technology

The present application relates to a kind of heat shock flash evaporation loop lift membrane heat exchange test system, including heat exchange pipe, vapor-liquid separator, condenser, metering barrel, vacuum pump, liquid tank, heat shock heater, throttle valve, measurement and control system and high-speed camera, the upper outlet of heat exchange pipe is connected with the import of vapor-liquid separator, the secondary steam outlet of vapor-liquid separator is connected with the hot medium side import of condenser, the hot medium side outlet of condenser is connected with metering barrel and liquid tank respectively, the concentrated liquid outlet of vapor-liquid separator is connected with liquid tank, the outlet of metering barrel is connected with liquid tank, vacuum pump is connected with liquid tank, liquid tank is connected with heat shock heater, heat shock heater is connected with heat exchange pipe, throttle valve is arranged between heat shock heater and heat exchange pipe;High-speed camera is arranged in one side of heat exchange pipe.The present application can realize the experimental test to heat shock flash evaporation loop lift membrane heat exchange technology, and provide support for mechanism research, equipment optimization of heat shock flash evaporation loop lift membrane heat exchange technology.

The present application relates to a kind of heat pump rectification systems based on heat shock flash circulation lift heat exchange, including rectifying tower, lift membrane reboiler, first throttling valve and heat shock heater, the secondary steam outlet of rectifying tower is connected with the heating steam import of lift membrane reboiler, the discharge port of the bottom of rectifying tower is connected with the cold side flow channel import of heat shock heater, and the cold side flow channel export of heat shock heater is connected with the feed inlet of lift membrane reboiler by first throttling valve;The vapor-liquid mixture outlet of lift membrane reboiler is connected with the column still liquid reflux port of rectifying tower, and the steam condensate outlet of lift membrane reboiler is connected with condensate tank, and the liquid outlet of condensate tank is connected with the condensate reflux port of rectifying tower side wall.The present application is combined by heat shock pressurization energy storage and throttling flash kinetic energy release, provides independent and stable lift membrane kinetic energy for lift membrane heat exchange, so that the flow pattern in the tube of lift membrane reboiler directly enters the efficient annular flow stage, improves the industrial applicability of lift membrane heat exchange technology in heat pump rectification system.

The present application relates to a kind of hot exciting flash evaporation loop lift heat exchanger, including upper head, lower head, cylinder, several heat exchange tubes, the upper end surface of cylinder and the upper head form cavity I between, the lower end surface of cylinder and the lower head form cavity II between, cavity I, cavity II and the inner cavity of cylinder are not communicated with each other between, heat exchange tube is fixed in the inside of cylinder by tube sheet, the upper end of heat exchange tube is placed in cavity I and is communicated with cavity I, lower end and cavity II are communicated, cylinder includes lift section cylinder and hot exciting section cylinder, heat exchange tube includes lift section heat exchange tube and hot exciting section heat exchange tube, lift section heat exchange tube and hot exciting section heat exchange tube are communicated by throttling component between, the inner diameter of throttling component is less than the inner diameter of hot exciting section heat exchange tube.The heat exchanger of the present application is provided with a kind of hot exciting flash evaporation loop lift, will hot exciting pressurization energy storage, throttling flash evaporation kinetic energy release, loop lift heat exchange and vapor-liquid separation function integration in same equipment, solve the problem of traditional lift section heat exchanger dependence big temperature difference.

This invention discloses a method for large-scale breeding of all-female triploid mandarin fish, comprising the following steps: (1) Preparation of all-female diploid fertilized eggs: using male mandarin fish pseudo-males (XX) induced by androgens and female mandarin fish (XX) selected from superior strains as parent fish, collecting sperm and eggs, mixing the two, adding water at a temperature of 28℃ to activate sperm for fertilization, and obtaining all-female diploid fertilized eggs; (2) Heat shock induction of triploids: after the all-female diploid fertilized eggs are fertilized in the above-mentioned water at a temperature of 28℃ for 8 minutes, they are immediately transferred to water at a temperature of 39~41℃ for 1~2 minutes for heat shock induction, and all-female triploid fertilized eggs are obtained; (3) Seedling hatching and cultivation: the fertilized eggs induced by heat shock in step (2) are transferred to the hatching system for hatching, and then conventional seedling cultivation is carried out to obtain all-female triploid mandarin fish seedlings. The mandarin fish fry produced by this method have a high female rate, triploid rate, and survival rate.

This invention discloses a method for efficiently inducing allopolyploid fish fry, belonging to the field of fish breeding technology. The method of this invention includes the following technical steps: (1) Induction of spawning in distant hybridization parents: Selecting female and male parent fish from different subfamilies of Cyprinidae, and collecting mature eggs and semen respectively; (2) Obtaining hybrid fertilized eggs: Mixing the collected eggs with semen, and performing dry artificial insemination to obtain hybrid fertilized eggs; (3) Inducing allopolyploidy: Placing the fertilized eggs in a water bath for heat shock treatment to inhibit the expulsion of the second polar body or inhibit the first cleavage, and then quickly transferring the treated fertilized eggs to aquaculture water to continue hatching fry. The method of this invention combines the method of distant hybridization between subfamilies with the method of heat shock induction. After induction using this method, the multiplication rate of hatched fry can reach 100%, which significantly improves the yield of allopolyploid fish fry from distant hybridization.

This invention relates to the field of aquatic product deep processing technology, and discloses a squid salad processing method based on gradient temperature cooking. The squid salad is made from cooked squid rings, mixed vegetables, and a salad dressing containing a specific compound emulsifying stabilizer. The processing method includes: subjecting pre-treated squid to acoustic-thermal coupled gradient cooking, sequentially undergoing three stages: ultrasonic-assisted low-temperature sol-gelation, silent medium-temperature heat-induced gelation, and high-temperature safe setting; subsequently, immediately immersing it in an ice-water mixture for gradient heat-blocking cooling, draining, and then mixing with the dressing. This invention effectively solves the problems of severe moisture loss and tough texture in traditional processes by precisely controlling the timing of ultrasonic intervention and three-stage temperature control, combined with the heat shock effect, giving the product a crispy exterior and tender interior texture. Simultaneously, the use of compound stabilizers improves the emulsification stability and water-holding capacity of the dressing in a high-humidity matrix, increasing the yield of the finished product.

This invention relates to the field of cosmetics, specifically to a composition containing copper peptides for repairing photothermal damage and its application. The composition comprises a complex polypeptide, galactosomal yeast fermentation product filtrate, and cyanobacterial extract; wherein the complex polypeptide includes copper peptides, yeast polypeptides, and hexapeptide-11. This invention, by combining specific complex polypeptides with galactosomal yeast fermentation product filtrate and cyanobacterial extract, increases the expression level of heat shock proteins, thereby achieving excellent efficacy in repairing photothermal damage. Furthermore, this composition is mild and non-irritating, and can be widely used in cosmetics.

Compounds, compositions and methods for improving the viability and / or function of cells or for the in vitro, ex vivo or in vivo protection of cells, tissue, graft or organs from various damages are described. The reagents and composition are based on activation of the heat shock response activation and / or the antioxidant response and include for example. HSP90 co-factor inhibitor such as Celastrol or Celastrol analogs used alone or in combination with an adjunct agent (e.g., a NRF-2 activator, antioxidant, etc.). Therapeutic enhancement may also include increase in paracrine effector production and signaling. Methods for improving the resistance of cells, tissue, grafts or organs to damages or stress, such as hypoxic or oxidative stress-induced cell death, and / or for improving the viability and retention of transplanted or transfused cells are also described. Therapeutic treatment or prevention of ischemic injury (e.g. myocardial infarct, ischemia / reperfusion injury) and related stressors (hypoxia, oxidative stress, inflammation, sepsis / shock, etc) are also provided.

The application discloses application of ZmGOLS2 genes and ZmRAFS genes in improving pollen vitality of corn under heat shock and drought conditions, and belongs to the technical field of genetic engineering. The application improves the pollen vitality of corn, promotes the germination of pollen under heat shock and drought stress conditions, and reduces the empty stalk rate of corn under drought stress conditions by simultaneously improving the expression levels of a corn myo-inositol galactoside synthetase coding gene and a corn raffinose synthetase coding gene.

A method is provided for applying a pulsed energy source to living tissue to stimulate the activation of heat shock proteins and promote protein repair without damaging the tissue. A method for thermally treating biological tissue includes providing a pulsed energy source having energy parameters selected to raise the target temperature to a level that achieves a therapeutic effect, while maintaining the increase in average temperature of the tissue over time below a predetermined level so as not to permanently damage the target tissue. Application of the pulsed energy source to the target tissue induces a heat shock response and stimulates the activation of heat shock proteins in the target tissue to therapeutically treat the target tissue.

The invention belongs to the technical field of biological medicines, and particularly relates to a membrane-coated oncolytic virus as well as a preparation method and application thereof. The oncolytic virus contains a GSDMD N-terminal expression cassette regulated and controlled by a heated shock promoter, and pyroptosis can be induced under exogenous mild thermal stimulation, so that the virus is promoted to be quickly released, and reinfection and amplification of adjacent tumor cells are realized. A bionic nano-vesicle (iNV) formed by a donor cell membrane modified by genetic engineering is used as an outer layer to coat a genetically engineered oncolytic virus (GOV) to obtain a membrane-coated oncolytic virus iNV-GOV, so that the membrane-coated oncolytic virus iNV-GOV simultaneously has immunocompatibility and active tumor recognition capability, is suitable for solid tumors such as pancreatic ductal adenocarcinoma (PDAC), hepatocellular carcinoma (HCC), intrahepatic cholangiocarcinoma (IHCC) and the like, and can be used for preparing an anti-tumor drug. And the tumor volume can be obviously reduced in mouse in-vivo model experiments.

The invention provides a method, a system, equipment and a medium for constructing a yeast high-temperature tolerance prediction model, and belongs to the field of microbial breeding, and the method comprises the following steps: determining a tolerance label based on a maximum specific growth rate, an ethanol yield and a final ethanol concentration, and obtaining transcriptome data and metabolome data of a saccharomyces cerevisiae strain, the metabolome data comprises the synthesis rate of the trehalose, and the synthesis rate of the trehalose is determined according to the net synthesis amount of the trehalose in the heat shock time of the saccharomyces cerevisiae strain; constructing feature vectors according to the transcriptome data and the metabolome data; and inputting the feature vectors into the model to determine a predicted tolerance score, and performing hyper-parameter optimization on the model according to the tolerance tag and the tolerance score of the saccharomyces cerevisiae strain in the training set to obtain a yeast high-temperature tolerance prediction model. According to the method, the built-in powerful nonlinear learning ability of the model is utilized, the complex mapping relation between the multi-omics characteristics and the high-temperature tolerance is mined, and the accuracy and reliability during model prediction are guaranteed.

Provided herein are methods of activating cells (e.g., CAR-T cells) by heat shock, e.g., by contacting the cells with pre-heated media. Also provided herein are methods of preserving the expression level of a protein of interest (e.g., a CAR) by freezing cells after activation. The frozen cells can be further thawed and recovered for optimal expression of the protein of interest (e.g., a CAR). The activated cells described herein can be administered to subjects in need thereof to treat diseases or disorders (e.g., cancer).

Provided are methods and systems for producing a cell based product for dietary consumption, methods and systems for selecting cell lines with heat shock inducible genes associated with a fat or muscle phenotype, draw and fill methods and systems, methods and systems for a chemostat with cell retention, chicken or bovine cell lines, and edible bovine or avian cell lines obtained from the methods described herein.

The present disclosure relates to compositions comprising modified heat shock proteins conjugated to a humanized 3E10 antibody or antigen-binding fragment thereof as well as methods of using the same to treat blast injury to the eye, brain, and cochlea.

The application relates to an MVR system based on a heat shock flash evaporation loop lift heat exchange, which comprises a lift heat exchanger, a vapor-liquid separator, a preheater, a heat shock heater and a throttling component, the preheater is communicated with the heat shock heater, the condensate inlet of the preheater is communicated with the condensate outlet of the lift heat exchanger; the material outlet of the heat shock heater is communicated with the feed inlet of the lift heat exchanger through the throttling component; the lift heat exchanger is communicated with the vapor-liquid separator, so that the secondary steam in the lift heat exchanger enters the vapor-liquid separator, the gaseous phase after separation is compressed and introduced into the lift heat exchanger, and is used as a heat source of the lift heat exchanger; the concentrated liquid flowing out of the vapor-liquid separator and the lift heat exchanger is extracted when the concentration detection is qualified, and is re-injected into the heat shock heater after being pressurized for reuse when the concentration detection is unqualified. The application can form a stable circular flow without relying on a large temperature difference, matches the narrow temperature difference heat transfer characteristics of the MVR system, and greatly reduces the high-grade energy consumption of the lift heat exchanger.

The utility model discloses a cold and hot shock test box which comprises an outer box body and an inner box body, the inner box body is located in the outer box body and connected with the outer box body, a box door is arranged on one side of the outer box body, and the inner box body and the box door form a test cavity; a first notch and a second notch are formed in the bottom of the test cavity, a first groove body is arranged below the first notch, and a second groove body is arranged below the second notch; a lifting basket capable of moving back and forth in the horizontal direction and the vertical direction is arranged in the test cavity, and the first notch and the second notch are both located in the stroke range of the lifting basket. According to the utility model, a product to be tested can be placed in the lifting baskets, and after the liquid in the tank body is heated to reach the required temperature, the lifting baskets are placed in the corresponding tank bodies through the transfer mechanism, so that automatic transfer operation is realized, and the test efficiency is high; different temperatures can be set for different tank bodies, tests can be continuously carried out, the temperature in the test cavity is constant, the thermal insulation performance of the tank bodies is good, and the accuracy of test results is high.

Provided are methods and systems for producing a cell based product for dietary consumption, methods and systems for selecting cell lines with heat shock inducible genes associated with a fat or muscle phenotype, draw and fill methods and systems, methods and systems for a chemostat with cell retention, chicken or bovine cell lines, and edible bovine or avian cell lines obtained from the methods described herein.

This invention discloses the Clostridium difficile phage recombinant lyase Lys51, its preparation method, and its application, relating to the field of genetic engineering technology. The invention involves introducing the recombinant plasmid pET-28a-SUMO-Lys51 into Escherichia coli BL21(DE3) competent cells via heat shock transformation; screening to obtain the recombinant engineered bacterium BL21(DE3)-pET-28a-SUMO-Lys51; constructing the recombinant plasmid pET-28a-SUMO-Lys51 and the recombinant engineered bacterium BL21(DE3)-pET-28a-SUMO-Lys51, further inducing the expression of Clostridium difficile phage recombinant lyase Lys51 in the recombinant engineered bacterium BL21(DE3)-pET-28a-SUMO-Lys51, and then purifying it to achieve the complete process for preparing Clostridium difficile phage recombinant lyase Lys51.

The invention relates to the technical field of heat management of air conditioning equipment, and discloses an air conditioner compressor operating environment optimization method based on radiation refrigeration, which comprises a phase change energy storage unit, a heat transfer loop unit and a self-adaptive system of a radiation heat dissipation unit. The phase change energy storage unit is used for absorbing heat of the compressor, and heat shock is buffered through phase change latent heat; a temperature control valve in the heat transport loop unit passively controls on or off of a loop based on a temperature threshold value, so that self-adaptive heat transport is realized; and the radiation heat dissipation unit radiates heat to the outside by utilizing a spectrum selective structure. Under a low-load or night working condition, thermal resetting of the phase change medium is realized through continuous heat dissipation, and surface attachments are removed by using condensed water generated on the surface in cooperation with the micro-nano composite super-hydrophobic structure. Through a multi-stage passive thermal control mechanism, the temperature fluctuation of the compressor is effectively stabilized, self-cleaning is realized by utilizing a condensation phenomenon, and the technical problem that the radiation heat dissipation performance is attenuated along with accumulated dust is solved.

The problem of heat shock, which occurs when the body cools down in a cold changing room or bathroom during bathing, causing a sudden increase in blood pressure, and then when entering a warm bathtub, causing a sudden drop in blood pressure, is addressed by providing a cold-weather coat. [Solution] This is a thermal coat for use when moving between a warm room or personal room in the house and the bathroom, instead of using a cold changing room. You wear the thermal coat while keeping your underwear on, and then take off the coat in the bathroom and shower in your underwear. This prevents a sudden rise in blood pressure due to the cold and a sudden drop in blood pressure when entering the bathtub, thus avoiding heat shock. When leaving the bathroom, dry your body with a face towel, put on the thermal coat, and move to a warm room or your own room to change.

This invention relates to the development of heat-tolerant soft-shell clam (Mya arenaria) lines for aquaculture through selective breeding and molecular genetic approaches. Two methods are employed: (1) phenotype-based selection, in which clams are exposed to natural or controlled heat shock conditions (30-33° C.) and survivors are selected across successive generations and multiple populations; and (2) marker-assisted selection (MAS), utilizing single nucleotide polymorphisms (SNPs) identified through genome-wide association studies (GWAS) to select broodstock capable of producing heat-tolerant offspring. The resulting lines exhibit enhanced survival and endurance under elevated temperatures, particularly above the species' critical threshold of 28° C. These heat-tolerant clams enable sustainable aquaculture production in Maryland and other warm, low-salinity estuaries where soft-shell clams historically failed to survive summer heat.

The invention belongs to the technical field of plant biology, and particularly relates to a garlic aerial bulb induction and seed garlic propagation method integrating detoxification and rapid propagation functions, which comprises the following steps: firstly, performing heat shock and chemical demethylation synergistic double detoxification on garlic cloves; inoculating the detoxified stem tips into an induction culture medium containing a specific plant growth regulator and a jasmonic acid methyl ester precursor to directionally form aerial bulbs; then, high-efficiency proliferation is realized through two-stage propagation culture under the regulation and control of light and temperature; finally, the salicylic acid derivative and the growth-promoting rhizobacteria are combined to strengthen the field resistance. According to the method, efficient detoxification, rapid propagation and lasting resistance reconstruction can be synchronously achieved, the propagation coefficient is 3 times or above that of a traditional method, and the two-season virus re-infection rate is lower than 15%.

This invention discloses a method for preparing liquid organic fertilizer based on a combined grinding-enzymatic hydrolysis process, belonging to the field of liquid organic fertilizer preparation technology. The method includes: coarsely screening livestock manure to remove impurities, then mixing it evenly with water to obtain a mixed slurry; mechanically grinding the mixed slurry to obtain an activated slurry; adding a compound enzyme preparation to the activated slurry to obtain an enzymatically hydrolyzed material; heating the enzymatically hydrolyzed material to obtain a heat-shocked material; performing solid-liquid separation on the heat-shocked material to obtain a high-temperature filtrate and a filter residue; adding water-soluble nitrogen, phosphorus, potassium, humic acid, and trace elements to the cooled filtrate, and inoculating with a microbial agent to obtain the liquid organic fertilizer based on the combined grinding-enzymatic hydrolysis process. This invention promotes the conversion of macromolecular nutrients in manure into soluble small molecules through the combined grinding-enzymatic hydrolysis process, while the heat shock treatment simultaneously terminates enzyme activity, inactivates pathogens, and reduces viscosity. Subsequent inoculation with beneficial bacteria ensures long-term survival, resulting in a liquid organic fertilizer that combines rapid-acting nutrition with microecological conditioning functions.

The invention relates to the technical field of fluid machinery, in particular to a centrifugal pump impeller of an integrated phase change material passive heat management structure, which comprises a passive cooling assembly arranged on a rear cover plate of the impeller, and the passive cooling assembly is used for passively cooling the rear cover plate; the passive cooling assembly comprises a plurality of closed cavities formed in the rear cover plate, and a plurality of radial straight fins arranged at equal intervals in the circumferential direction are arranged on the inner walls of the closed cavities. The closed cavity is filled with phase change materials, and the phase change materials make contact with the outer walls of the radial straight fins. The phase change material is integrated in the impeller, effective heat management is carried out in the initial stage of heat conduction, temperature changes are quickly responded, heat shock is effectively buffered, source heat protection of a bearing and a sealing system is achieved, and the service life of the bearing and the service life of the sealing system are remarkably prolonged.

The invention discloses an application of a corn pseudorhombus protein ZmDerlin3 gene and an encoded protein thereof in heat-resistant breeding of plants, and belongs to the technical field of genetic engineering. The invention clones a corn pseudorhombus protein ZmDerlin3 gene, the nucleotide sequence of the corn pseudorhombus protein ZmDerlin3 gene is shown as SEQ ID NO.1, and the amino acid sequence of the corn pseudorhombus protein ZmDerlin3 gene is shown as SEQ ID NO.2. The invention provides a corn pseudorhombus protein ZmDerlin3 gene and an application of an encoding protein of the corn pseudorhombus protein ZmDerlin3 gene in heat-resistant breeding of plants. A heat shock experiment shows that the growth phenotype of a corn seedling overexpressed with ZmDerlin3 is obviously superior to that of a wild type, that is, the ZmDerlin3 gene positively regulates the heat resistance of the corn. The gene provided by the invention has important application value for cultivating a new high-yield heat-resistant corn variety.

The present invention relates to a protein composition of Arabidopsis thaliana propilin 2 (AtPFN2) that acts as a molecular chaperone under heat shock stress. More specifically, the invention relates to a protein composition of Arabidopsis thaliana propilin 2 (AtPFN2) that protects a plant from heat shock by acting as a molecular chaperone under heat shock stress and converting into a high molecular weight complex.

This invention discloses a bioactive composition with synergistic effects. The composition comprises a highly active stem cell extract prepared by a sequential pretreatment of hypoxia combined with heat shock, and a high-affinity monoclonal antibody P3F6 targeting human PTEN protein. The stem cell extract is rich in heat shock proteins and various growth factors; the monoclonal antibody P3F6 possesses specific light and heavy chain variable region sequences. Experiments have demonstrated that this composition exhibits significant synergistic effects in promoting skin cell proliferation, inhibiting apoptosis, enhancing skin barrier function, and promoting collagen synthesis, with effects far exceeding those of single components. This invention also provides the application of the composition in the preparation of skin repair, anti-aging cosmetics, and medical wound healing dressings.

The blood cooling device provided by the application comprises a first cooling unit, a second cooling unit, a third cooling unit and a fourth cooling unit, any one of the cooling units comprises a constant-temperature cavity, a blood pipeline arranged in the constant-temperature cavity and a composite phase change material distributed in the constant-temperature cavity, the blood cooling device provided by the application utilizes four kinds of composite phase change cold storage materials with gradient distribution of melting points, so that the blood passing through the blood cooling device is cooled in four stages, the temperature difference between the blood and the cold surface in the whole cooling process does not exceed 15 DEG C, the blood temperature before and after each stage of cooling can be fixed in a preset range, and finally the output temperature is about 4 DEG C. Since the phase change material has natural energy storage and cold storage capacity, the cold quantity can be released when needed, so that the ability of the device to cope with different working conditions and abnormal cold / heat shocks caused by factors such as faults is enhanced.