123 results about "Heat shock" patented technology

The present application relates to a kind of heat shock flash evaporation loop lift membrane heat exchange test system, including heat exchange pipe, vapor-liquid separator, condenser, metering barrel, vacuum pump, liquid tank, heat shock heater, throttle valve, measurement and control system and high-speed camera, the upper outlet of heat exchange pipe is connected with the import of vapor-liquid separator, the secondary steam outlet of vapor-liquid separator is connected with the hot medium side import of condenser, the hot medium side outlet of condenser is connected with metering barrel and liquid tank respectively, the concentrated liquid outlet of vapor-liquid separator is connected with liquid tank, the outlet of metering barrel is connected with liquid tank, vacuum pump is connected with liquid tank, liquid tank is connected with heat shock heater, heat shock heater is connected with heat exchange pipe, throttle valve is arranged between heat shock heater and heat exchange pipe;High-speed camera is arranged in one side of heat exchange pipe.The present application can realize the experimental test to heat shock flash evaporation loop lift membrane heat exchange technology, and provide support for mechanism research, equipment optimization of heat shock flash evaporation loop lift membrane heat exchange technology.

The present application relates to a kind of heat pump rectification systems based on heat shock flash circulation lift heat exchange, including rectifying tower, lift membrane reboiler, first throttling valve and heat shock heater, the secondary steam outlet of rectifying tower is connected with the heating steam import of lift membrane reboiler, the discharge port of the bottom of rectifying tower is connected with the cold side flow channel import of heat shock heater, and the cold side flow channel export of heat shock heater is connected with the feed inlet of lift membrane reboiler by first throttling valve;The vapor-liquid mixture outlet of lift membrane reboiler is connected with the column still liquid reflux port of rectifying tower, and the steam condensate outlet of lift membrane reboiler is connected with condensate tank, and the liquid outlet of condensate tank is connected with the condensate reflux port of rectifying tower side wall.The present application is combined by heat shock pressurization energy storage and throttling flash kinetic energy release, provides independent and stable lift membrane kinetic energy for lift membrane heat exchange, so that the flow pattern in the tube of lift membrane reboiler directly enters the efficient annular flow stage, improves the industrial applicability of lift membrane heat exchange technology in heat pump rectification system.

The present application relates to a kind of hot exciting flash evaporation loop lift heat exchanger, including upper head, lower head, cylinder, several heat exchange tubes, the upper end surface of cylinder and the upper head form cavity I between, the lower end surface of cylinder and the lower head form cavity II between, cavity I, cavity II and the inner cavity of cylinder are not communicated with each other between, heat exchange tube is fixed in the inside of cylinder by tube sheet, the upper end of heat exchange tube is placed in cavity I and is communicated with cavity I, lower end and cavity II are communicated, cylinder includes lift section cylinder and hot exciting section cylinder, heat exchange tube includes lift section heat exchange tube and hot exciting section heat exchange tube, lift section heat exchange tube and hot exciting section heat exchange tube are communicated by throttling component between, the inner diameter of throttling component is less than the inner diameter of hot exciting section heat exchange tube.The heat exchanger of the present application is provided with a kind of hot exciting flash evaporation loop lift, will hot exciting pressurization energy storage, throttling flash evaporation kinetic energy release, loop lift heat exchange and vapor-liquid separation function integration in same equipment, solve the problem of traditional lift section heat exchanger dependence big temperature difference.

The invention discloses a dynamic permeability testing system and method for hydrate core production by a pressure reduction or heat shock method, and the system comprises a dynamic permeability testing unit (1) which is used for the preparation of a natural gas hydrate core sample and the measurement of permeability; the gas and liquid injection unit (2) is used for injecting required gas and liquid into the dynamic permeability testing system; the hydraulic oil control unit (3) is used for applying axial pressure and confining pressure to the dynamic permeability testing system; the gas-liquid separation metering unit (4) is used for metering the gas-liquid flow at the outlet; and the data acquisition monitoring and control unit (5) is used for monitoring data of each unit and controlling each unit to work. According to the invention, the natural gas hydrate-containing rock core can be subjected to pressure drop under the triaxial stress condition or the production dynamic permeability test by a heat shock method.

The application provides a steam condensate waste heat recycling device, and relates to the field of heat energy recovery.The steam condensate waste heat recycling device comprises a base, a heat preservation cylinder arranged on the base, and a synchronous adjusting mechanism arranged on the heat preservation cylinder.The steam condensate waste heat recycling device can conveniently collect condensate water into a required device, and the high-temperature condensate water can not lose a large amount of heat through a pipeline and a surface of equipment during a transmission process, so that heat energy is not wasted.The synchronous adjusting mechanism drives a conveying pump to quickly transfer the condensate water in the heat preservation cylinder to a required place, so that the condensate water recovery efficiency is improved.The synchronous adjusting mechanism solves the problem of waste of condensate water waste heat in the prior art.The synchronous adjusting mechanism solves the problem of equipment damage caused by heat shock in the prior art.The synchronous adjusting mechanism solves the problem of increased thermal resistance caused by scale accumulation in the prior art.

This invention discloses a method for large-scale breeding of all-female triploid mandarin fish, comprising the following steps: (1) Preparation of all-female diploid fertilized eggs: using male mandarin fish pseudo-males (XX) induced by androgens and female mandarin fish (XX) selected from superior strains as parent fish, collecting sperm and eggs, mixing the two, adding water at a temperature of 28℃ to activate sperm for fertilization, and obtaining all-female diploid fertilized eggs; (2) Heat shock induction of triploids: after the all-female diploid fertilized eggs are fertilized in the above-mentioned water at a temperature of 28℃ for 8 minutes, they are immediately transferred to water at a temperature of 39~41℃ for 1~2 minutes for heat shock induction, and all-female triploid fertilized eggs are obtained; (3) Seedling hatching and cultivation: the fertilized eggs induced by heat shock in step (2) are transferred to the hatching system for hatching, and then conventional seedling cultivation is carried out to obtain all-female triploid mandarin fish seedlings. The mandarin fish fry produced by this method have a high female rate, triploid rate, and survival rate.

The application discloses a kind of cultivation methods of konjak, belong to konjak planting technical field, the present application is to the pretreatment konjak seed potato of sterilization in nitrogen and ozone mixed atmosphere steam treatment, not only strengthen the growth potential of konjak seed potato, and fully activated the catalase in seed potato, create excellent intracellular environment for promoting subsequent seed potato germination, heat shock seed potato is soaked in activation liquid and activated, brassinolide, calcium sulfate, ethiofencarb and sodium complex nitrophenol in activation liquid activate the cytokinin oxidase in cell, and then regulate seed potato germination speed and germination quality, effectively solve the problem of low seedling rate of offspring konjak and yield decline.The activation seed potato is sowed in nutrient soil, effectively improves the content of nutrient substance in harvested konjak.

This invention discloses a method for efficiently inducing allopolyploid fish fry, belonging to the field of fish breeding technology. The method of this invention includes the following technical steps: (1) Induction of spawning in distant hybridization parents: Selecting female and male parent fish from different subfamilies of Cyprinidae, and collecting mature eggs and semen respectively; (2) Obtaining hybrid fertilized eggs: Mixing the collected eggs with semen, and performing dry artificial insemination to obtain hybrid fertilized eggs; (3) Inducing allopolyploidy: Placing the fertilized eggs in a water bath for heat shock treatment to inhibit the expulsion of the second polar body or inhibit the first cleavage, and then quickly transferring the treated fertilized eggs to aquaculture water to continue hatching fry. The method of this invention combines the method of distant hybridization between subfamilies with the method of heat shock induction. After induction using this method, the multiplication rate of hatched fry can reach 100%, which significantly improves the yield of allopolyploid fish fry from distant hybridization.

This invention relates to the field of aquatic product deep processing technology, and discloses a squid salad processing method based on gradient temperature cooking. The squid salad is made from cooked squid rings, mixed vegetables, and a salad dressing containing a specific compound emulsifying stabilizer. The processing method includes: subjecting pre-treated squid to acoustic-thermal coupled gradient cooking, sequentially undergoing three stages: ultrasonic-assisted low-temperature sol-gelation, silent medium-temperature heat-induced gelation, and high-temperature safe setting; subsequently, immediately immersing it in an ice-water mixture for gradient heat-blocking cooling, draining, and then mixing with the dressing. This invention effectively solves the problems of severe moisture loss and tough texture in traditional processes by precisely controlling the timing of ultrasonic intervention and three-stage temperature control, combined with the heat shock effect, giving the product a crispy exterior and tender interior texture. Simultaneously, the use of compound stabilizers improves the emulsification stability and water-holding capacity of the dressing in a high-humidity matrix, increasing the yield of the finished product.

The application discloses a low-temperature constant-temperature cold source utilizing adsorption latent heat, which is provided with an adsorption container filled with an adsorbent and filled with a working gas, and utilizes the adsorption latent heat generated when the working gas is desorbed to provide the cold source. The low-temperature constant-temperature cold source utilizes the adsorption latent heat generated when the working gas is desorbed to provide the cold source for a cooling platform. The application does not have any active moving parts, and has the characteristic of no vibration; the adsorption of the working gas on the surface of the adsorbent can occur in a wide temperature range higher than the boiling point of the working gas, so that the cooling can be provided in a wide temperature range; the utilization of the adsorption latent heat as the cold source can cope with greater heat shock, has better temperature stability, and has the advantages of simple device structure, easy-to-obtain material, convenient operation, low cost and the like.

The utility model discloses an experimental device for detecting the thermal shock performance of a protective coating. The experimental device comprises an experimental table, a cooling water tank, a motor, a turntable, a connecting rod and a flame spray gun, the experiment table comprises a frame body, a table plate and a supporting plate; an avoiding hole is formed in the table plate, the bottom end of the cooling water tank is fixed to the supporting plate, and the top end of the cooling water tank upwards penetrates out of the avoiding hole and is provided with a cooling water filling opening; the motor is fixed on the bedplate; the turntable is positioned above the cooling water filling port and is in transmission connection with an output shaft of the motor; the connecting rods are radially arranged along the turntable, first ends of the connecting rods are fixed on the turntable, clamps are fixed at second ends of the connecting rods, and the clamps can clamp a to-be-tested metal piece coated with a protective coating; the flame spraying gun is installed on the platen, and the flame spraying end of the flame spraying gun faces the rotating disc. The experimental device for detecting the thermal shock performance of the protective coating disclosed by the utility model is time-saving and labor-saving, and a metal piece to be detected coated with the protective coating can be rapidly and repeatedly heated and cooled.

This invention relates to the field of cosmetics, specifically to a composition containing copper peptides for repairing photothermal damage and its application. The composition comprises a complex polypeptide, galactosomal yeast fermentation product filtrate, and cyanobacterial extract; wherein the complex polypeptide includes copper peptides, yeast polypeptides, and hexapeptide-11. This invention, by combining specific complex polypeptides with galactosomal yeast fermentation product filtrate and cyanobacterial extract, increases the expression level of heat shock proteins, thereby achieving excellent efficacy in repairing photothermal damage. Furthermore, this composition is mild and non-irritating, and can be widely used in cosmetics.

Compounds, compositions and methods for improving the viability and / or function of cells or for the in vitro, ex vivo or in vivo protection of cells, tissue, graft or organs from various damages are described. The reagents and composition are based on activation of the heat shock response activation and / or the antioxidant response and include for example. HSP90 co-factor inhibitor such as Celastrol or Celastrol analogs used alone or in combination with an adjunct agent (e.g., a NRF-2 activator, antioxidant, etc.). Therapeutic enhancement may also include increase in paracrine effector production and signaling. Methods for improving the resistance of cells, tissue, grafts or organs to damages or stress, such as hypoxic or oxidative stress-induced cell death, and / or for improving the viability and retention of transplanted or transfused cells are also described. Therapeutic treatment or prevention of ischemic injury (e.g. myocardial infarct, ischemia / reperfusion injury) and related stressors (hypoxia, oxidative stress, inflammation, sepsis / shock, etc) are also provided.

The application discloses application of ZmGOLS2 genes and ZmRAFS genes in improving pollen vitality of corn under heat shock and drought conditions, and belongs to the technical field of genetic engineering. The application improves the pollen vitality of corn, promotes the germination of pollen under heat shock and drought stress conditions, and reduces the empty stalk rate of corn under drought stress conditions by simultaneously improving the expression levels of a corn myo-inositol galactoside synthetase coding gene and a corn raffinose synthetase coding gene.

A method is provided for applying a pulsed energy source to living tissue to stimulate the activation of heat shock proteins and promote protein repair without damaging the tissue. A method for thermally treating biological tissue includes providing a pulsed energy source having energy parameters selected to raise the target temperature to a level that achieves a therapeutic effect, while maintaining the increase in average temperature of the tissue over time below a predetermined level so as not to permanently damage the target tissue. Application of the pulsed energy source to the target tissue induces a heat shock response and stimulates the activation of heat shock proteins in the target tissue to therapeutically treat the target tissue.

The invention provides a preparation method of a high-protein high-fiber zero-fat bionic eye fish maw, which comprises the following steps: mixing pea protein isolate, bone gelatin and water, adding glutamine transaminase and a compound flavor enzyme preparation, reacting at 50-60 DEG C for 30-60 minutes, then adding spirulina powder, and uniformly dispersing to obtain a protein base material; mixing and swelling konjaku flour, apple pectin and warm water, adding kappa-carrageenan, ultramicro kelp powder, calcium chloride and sodium carbonate, and homogenizing at 55-65 DEG C to form a gel matrix, so as to obtain a fiber base material; the method comprises the following steps: feeding a protein base material and a fiber base material through two independent feeding ports of a twin-screw extruder, and co-extruding through a die head with a plurality of layers of alternate runners to form a composite blank with alternate protein layers and fiber layers; and carrying out directional stretching and calcification, steam heat shock and flavor solidification, and cooling and shaping to obtain the bionic eye fish maw. By adopting the technical scheme, the protein content is more than or equal to 60% (dry basis), the dietary fiber content is more than or equal to 15%, and the fat content is less than or equal to 0.5%.

The invention belongs to the technical field of biological medicines, and particularly relates to a membrane-coated oncolytic virus as well as a preparation method and application thereof. The oncolytic virus contains a GSDMD N-terminal expression cassette regulated and controlled by a heated shock promoter, and pyroptosis can be induced under exogenous mild thermal stimulation, so that the virus is promoted to be quickly released, and reinfection and amplification of adjacent tumor cells are realized. A bionic nano-vesicle (iNV) formed by a donor cell membrane modified by genetic engineering is used as an outer layer to coat a genetically engineered oncolytic virus (GOV) to obtain a membrane-coated oncolytic virus iNV-GOV, so that the membrane-coated oncolytic virus iNV-GOV simultaneously has immunocompatibility and active tumor recognition capability, is suitable for solid tumors such as pancreatic ductal adenocarcinoma (PDAC), hepatocellular carcinoma (HCC), intrahepatic cholangiocarcinoma (IHCC) and the like, and can be used for preparing an anti-tumor drug. And the tumor volume can be obviously reduced in mouse in-vivo model experiments.

Compounds, compositions and methods for improving the viability and / or function of cells or for the in vitro, ex vivo or in vivo protection of cells, tissue, graft or organs from various damages are described. The reagents and composition are based on activation of the heat shock response and / or the antioxidant response and include for example, HSP90 co-factor inhibitor such as Celastrol or Celastrol analogs used alone or in combination with an adjunct agent (e.g., a NRF-2 activator, antioxidant, etc.). Therapeutic enhancement may also include increase in paracrine effector production and signaling. Methods for improving the resistance of cells, tissue, grafts or organs to damages or stress, such as hypoxic or oxidative stress-induced cell death, and / or for improving the viability and retention of transplanted or transfused cells are also described. Therapeutic treatment or prevention of ischemic injury (e.g. myocardial infarct, ischemia / reperfusion injury) and related stressors (hypoxia, oxidative stress, inflammation, sepsis / shock, etc) are also provided.

The invention relates to the use of the compound ES3707 as a therapeutic agent for the treatment of cancer diseases and for alleviating symptoms caused by such diseases, specifically by inhibiting heat shock proteins involved in cell division and by preventing resistance induced by clinical drugs. The therapeutic agent subject to the invention is characterized by its enhanced efficacy when used in combination with photodynamic therapy.

The invention discloses a preparation method of high-purity TIR2 and T1R3 recombinant plasmids. The preparation method comprises the following steps: transforming the T1R2 and T1R3 recombinant plasmids into modified Trans1-T1 escherichia coli competent cells through a heat shock method; large-scale amplification of plasmids is carried out by utilizing the efficient cloning characteristic of Trans1-T1 escherichia coli; and extracting and purifying recombinant plasmids from thalli by adopting an optimized alkali cracking combined column purification technology. According to the preparation method of the high-purity TIR2 and T1R3 recombinant plasmids, target DNA fragments or recombinant DNA plasmids constructed by the target DNA fragments are introduced into competent cells, amplification of genetic materials is achieved through a replication and expression system of host cells, finally the high-purity DNA plasmids are obtained through extraction and purification, and the high-purity TIR2 and T1R3 recombinant plasmids are obtained by improving transformation, amplification and purification processes. The yield and the purity of the T1R2 / T1R3 recombinant plasmid are remarkably improved, and the high-standard requirement of a transfection experiment is met.

The invention provides a method, a system, equipment and a medium for constructing a yeast high-temperature tolerance prediction model, and belongs to the field of microbial breeding, and the method comprises the following steps: determining a tolerance label based on a maximum specific growth rate, an ethanol yield and a final ethanol concentration, and obtaining transcriptome data and metabolome data of a saccharomyces cerevisiae strain, the metabolome data comprises the synthesis rate of the trehalose, and the synthesis rate of the trehalose is determined according to the net synthesis amount of the trehalose in the heat shock time of the saccharomyces cerevisiae strain; constructing feature vectors according to the transcriptome data and the metabolome data; and inputting the feature vectors into the model to determine a predicted tolerance score, and performing hyper-parameter optimization on the model according to the tolerance tag and the tolerance score of the saccharomyces cerevisiae strain in the training set to obtain a yeast high-temperature tolerance prediction model. According to the method, the built-in powerful nonlinear learning ability of the model is utilized, the complex mapping relation between the multi-omics characteristics and the high-temperature tolerance is mined, and the accuracy and reliability during model prediction are guaranteed.

Provided herein are methods of activating cells (e.g., CAR-T cells) by heat shock, e.g., by contacting the cells with pre-heated media. Also provided herein are methods of preserving the expression level of a protein of interest (e.g., a CAR) by freezing cells after activation. The frozen cells can be further thawed and recovered for optimal expression of the protein of interest (e.g., a CAR). The activated cells described herein can be administered to subjects in need thereof to treat diseases or disorders (e.g., cancer).

The utility model is suitable for the technical field of nuclear power station pressure control, and provides a detachable spraying assembly and a pressure stabilizer, the detachable spraying assembly comprises a spraying connecting pipe, a thermal shock prevention sleeve, a spray head connecting pipe and a spray head, a spraying cavity is formed in the spraying connecting pipe, and a first channel communicating the spraying cavity with the external space is formed in the spraying connecting pipe; the first end of the thermal shock prevention sleeve is arranged in the first channel, a gap is formed between the first end of the thermal shock prevention sleeve and the inner wall of the spraying connecting pipe, the second end of the thermal shock prevention sleeve is arranged in the spraying cavity, the spray head connecting pipe is arranged in the spraying cavity, the thermal shock prevention sleeve is arranged in a sleeved mode, the spray head connecting pipe is detachably connected with the second end of the thermal shock prevention sleeve, and the spray head is arranged in the spraying cavity. The nozzle connecting pipe is communicated with one end deviating from the first channel. According to the thermal shock prevention sleeve, the whole thermal shock prevention sleeve can be detached, when the thermal shock prevention sleeve is damaged or deformed, the whole thermal shock prevention sleeve is allowed to be detached and replaced more conveniently, the detachment and maintenance time can be shortened, and the operation safety of a unit is guaranteed.

Described herein are UV-resistant or UV-protective biological devices and extracts produced therefrom. The biological devices include microbial cells transformed with a DNA construct containing genes for producing UV-resistant proteins such as, for example, hexokinase, heat shock proteins, alcohol dehydrogenase, transferrin, flavonol synthase, zinc oxidase, and iron oxidase. Methods for producing and using the devices are also described herein. Finally, compositions and methods for using the devices and extracts to reduce or prevent UV-induced damage or exposure to materials, items, plants, and human and animal subjects are described herein.

The application discloses a hydrogel microneedle patch with combined anti-inflammatory and hyperthermia effects, and a preparation method and application thereof. The preparation method comprises the following steps: mixing polydopamine nanoparticles loaded with drugs and a biocompatible material solution containing a photoinitiator to obtain a pre-gel solution; dropping the pre-gel solution into a mold, and then performing drying and blue light crosslinking to obtain the hydrogel microneedle patch. The polydopamine nanoparticles used are excellent photothermal agents, and have a high drug loading rate. The hydrogel microneedle patch can also be subjected to freezing strengthening treatment to improve the mechanical strength, so that the needle tip can effectively penetrate into an intervertebral disc. The hydrogel microneedle patch can effectively inhibit inflammatory reactions. Meanwhile, hyperthermia can improve the level of heat shock proteins in cells and improve the self-protection of the cells. The hydrogel microneedle patch can promote the release of drugs by near-infrared light stimulation, improve the microenvironment, and meanwhile, a suitable temperature can promote the repair of tissues, so that the hydrogel microneedle patch has a good application prospect in the treatment of intervertebral disc degeneration diseases.

Provided are methods and systems for producing a cell based product for dietary consumption, methods and systems for selecting cell lines with heat shock inducible genes associated with a fat or muscle phenotype, draw and fill methods and systems, methods and systems for a chemostat with cell retention, chicken or bovine cell lines, and edible bovine or avian cell lines obtained from the methods described herein.

The present disclosure relates to compositions comprising modified heat shock proteins conjugated to a humanized 3E10 antibody or antigen-binding fragment thereof as well as methods of using the same to treat blast injury to the eye, brain, and cochlea.

The present invention relates to an inhibitor of heat shock protein 90, and discloses a bivalent inhibitor based on heat shock protein 90, a preparation method, and an application thereof. The compound has a structural formula as shown in Formula I: A-L-B Formula I, or a pharmaceutically acceptable salt, solvate, or optical isomer thereof, wherein A and B are ATP inhibitors of heat shock protein 90, and the motif structure of L is a flexible linking group of PEG and alkane, or a rigid linking group having an aryl, heteroalkyl, or heteroaryl group. The bivalent inhibitor can effectively inhibit the activity of the molecular chaperone protein HSP90, hinder the folding and modification of substrate proteins, degrade substrate proteins through the ubiquitin-proteasome pathway, induce non-native dimerization of HSP90, and interfere with protein-protein interactions associated with HSP90. At the same time, it also reduces the heat shock effect caused by HSP90 inhibition, and exhibits potent activity in degrading substrate proteins.

This invention belongs to the field of vegetable molecular biology technology, specifically relating to a method and kit for extracting heat shock nucleoproteins from protoplasts of leafy vegetables. The kit provided by this invention includes reagents A1, A2, B1, B2, B3, B4, and C; reagent A1 contains dissociative enzymes, cellulase, etc.; reagent A2 is a cell preservation and cell processing buffer; reagent B1 contains ethylenediaminetetraacetic acid, benzyl sulfonyl fluoride, etc.; reagent B2 contains Triton X-100, etc.; reagent B3 contains Glycerol, etc.; reagent B4 is a cell nucleus preservation solution; and reagent C is a cell nucleus lysis buffer. This kit enables precise extraction of heat shock nucleoproteins at the cellular level, yielding high-quality heat shock nucleoproteins and providing technical support for studying the expression and regulation of nuclear proteins in leafy vegetable cells.