21 results about "Streptococcus pyogenes" patented technology

The invention belongs to the technical field of microorganisms, and relates to a strain of herbaspirillum as well as a fermentation method and application thereof. The invention provides a strain of brassica juncea, the brassica juncea is brassica juncea YLHb25.7, the brassica juncea is named as Herbaspiril huttiense, the brassica juncea is preserved in China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.35297. The invention also provides a preparation method of the brassica juncea. The herbispirillum sp. YLHb25.7 disclosed by the invention has the capabilities of producing ACC deaminase, producing IAA and fixing nitrogen, and has good drought resistance and saline-alkaline tolerance. The herbaspirillum sp. YLHb25.7 and the fermentation product thereof can promote the growth of wheat and corn, can be used as a microbial agent or bacterial fertilizer, and can be applied to the agricultural market.

Compositions comprising 5. salivarius strain SALI-10 and / or salivarius 10 peptide are taught. Also taught are methods of treating bacterial infections comprising administering a pharmaceutically acceptable amount of the composition to the patient with a bacterial infection caused by a pathogen from, for example, multi-drug resistant (MDR) Streptococcus pneumoniae, Streptococcus pyogenes, Streptococcus dysagalactiae, vancomycin-resistant Enterococcus faecium, Porphyromonas gingivalis, Tannerella forsythia, and MDR Enterococcus faecalis.

The invention provides a strain of high-temperature-resistant and inflammation-relieving Witzia ciliaris and an application thereof, the high-temperature-resistant and inflammation-relieving Witzia ciliaris is Witzia ciliaris CN985 and is preserved in the China Center for Type Culture Collection (CCTCC), the preservation date is April 7, 2024, and the preservation number is CCTCC NO: M 2024641. The invention further provides a preparation method of the high-temperature-resistant and inflammation-relieving Witzia ciliaris strain for preventing and treating inflammation of the high-temperature-resistant and inflammation-relieving Witzia ciliaris strain for preventing and treating inflammation of the high-temperature-resistant and inflammation-relieving Witzia ciliaris strain. The invention provides a brand new strain of Witzia ciliaris CN985, which is resistant to high temperature, has better acid resistance and bile resistance, and has no hemolysis risk when being applied to a human body. The Witzia coagulans CN985 has excellent antibacterial performance, and especially can significantly inhibit streptococcus pyogenes, salmonella enteritidis and / or group B streptococcus; besides, experimental results show that the Witzia ciliaris CN985 can relieve enteritis, improve the immunity of the organism and relieve pharyngitis, and especially when the Witzia ciliaris CN985 and the phytobacterium plantarum CE18 are combined for use, the Witzia ciliaris CN985 and the phytobacterium plantarum CE18 have a synergistic interaction effect, so that the relieving effect on enteritis can be remarkably improved.

This invention discloses a strain of Bifidobacterium animalis subspecies TRT-2A with allergy-relieving effects and its extracellular vesicle applications. The strain TRT-2A is deposited at the China Center for Type Culture Collection (CCTCC) on November 21, 2025, with accession number CCTCC NO:M 20252645. This strain is highly acid-resistant and exhibits good tolerance to artificial gastric and intestinal fluids. It effectively inhibits bacteria, particularly Streptococcus pyogenes, Streptococcus agalactiae, and / or Escherichia coli. Experimental results show that this strain can alleviate hormone-dependent dermatitis and effectively improve dermatitis symptoms such as erythema and desquamation. Furthermore, extracellular vesicles prepared using TRT-2A can effectively improve skin elasticity and reduce wrinkles. Therefore, the Bifidobacterium animalis subspecies TRT-2A of this invention has multiple functions and is worthy of further promotion and application.

The application discloses a super-sensitive detection composition of aquatic product pathogenic bacteria and an application and a detection method thereof, and belongs to the technical field of biotechnology.The composition comprises an amplification primer with a sequence shown as SEQ ID No.1-2, a hairpin probe H1 with a fluorescent group and a quenching group modified on a nucleotide sequence shown as SEQ ID No.3, and a hairpin probe H2 with a sequence shown as SEQ ID No.4.Compared with conventional detection technologies such as a bacterial culture method, an instrument analysis method, a molecular biology method and an immunology method, the application provides a set of amplification primers and hairpin probes based on HCR, has higher specificity when detecting escherichia coli of aquatic products, and can avoid the influence of staphylococcus aureus, streptococcus pyogenes, pseudomonas aeruginosa and salmonella; meanwhile, the application has better sensitivity, and the detection limit can reach 0.4 CFU / mL; in addition, compared with other hairpin probes, the application can avoid false positives and improve reaction efficiency.

The present application relates to a Pichia pastoris indirect surface display system based on SpyC / T, construction and application thereof, and relates to the technical field of bioengineering. The Pichia pastoris indirect surface display system uses SED1 protein from Saccharomyces cerevisiae as an anchor protein, and fixes SpyC from Streptococcus pyogenes on the anchor protein on the surface of Pichia pastoris cells. The present application uses SED1 protein from Saccharomyces cerevisiae as an anchor protein, displays SpyC on the surface of Pichia pastoris, fixes various target proteins containing SpyTag on the cell surface through strong interaction of SpyC / T, so as to indirectly display the target proteins containing SpyTag on the surface of Pichia pastoris, exert the function of the target proteins containing SpyTag, and improve the stability of the target proteins.

Streptococcus pyogenes Cas9 (SpCas9) variants with relaxed PAM requirements capable of high-resolution editing for various applications, and methods of use thereof.

The present invention relates to methods of treating diseases in a subject in need thereof, including proliferative diseases such as cancer, especially solid tumors, as well as other diseases involving invasive cells or entities such as viruses, bacteria, fungi, and parasites, using IgG antibodies, including anti-tumor IgG antibodies, and a protease from Streptococcus pyogenes.

Described herein are compositions, methods, and kits for detecting wound pathogens. One embodiment described herein is primer pairs and probes for individual, simultaneous, or sequential multiplex polymerase chain reaction (PCR) based assays for the detection of Acinetobacter baumannii, Bacteroides fragilis, Clostridium perfringens, Enterobacter cloacae, Enterococcus faecalis, Enterococcus faecium, Escherichia coli, Klebsiella pneumoniae, Klebsiella Oxytoca, Klebsiella aerogenes, Proteus mirabilis, Proteus vulgaris, Pseudomonas aeruginosa, Serratia marcescens, Staphylococcus aureus, Streptococcus agalactiae, Streptococcus dysgalactiae, Streptococcus pyogenes, Streptococcus anginosus, Staphylococcus lugdunensis, Providencia stuartii, Stenotrophomonas maltophilia, Morganella morganii, Citrobacter koseri, Citrobacter freundii, Citrobacter braakii, Peptoniphilus asaccharolyticus, Peptoniphilus harei, Peptoniphilus ivorii, Peptostreptococcus anaerobius, Finegoldia magna, Clostridium septicum, Paeniclostridium sordellii (Clostridium sordellii), Clostridium novyi A, B, Clostridium histolyticum, Staphylococcus epidermidis, Staphylococcus haemolyticus, Corynebacterium striatum, Candida albicans, Candida auris, Candida glabrata, Candida krusei, Candida parapsilosis, or Candida tropicalis.

The present disclosure relates to methods of treating triple negative breast cancer or non-muscle invasive bladder cancer in a subject comprising administering to the subject a composition comprising non-viable cells of Streptococcus pyogenes and an immune checkpoint inhibitor.

The present disclosure relates to a method for purifying an immunoglobulin degrading enzyme (IdeS) from Streptococcus pyogenes, preferably to a method for removing IdeS charge variants and IdeS obtainable from said method.

This invention relates to the field of microbial technology, and provides a composite microbial suspension comprising a *Streptococcus pyogenes* R3 suspension and a *Cyclocarya pallida* N4 suspension in a volume ratio of 1-2:1-2; the *Streptococcus pyogenes* R3 classification name is... Herbaspirillum sp. R3 was deposited on September 20, 2023, at the China Center for Type Culture Collection (CCTCC), Wuhan University, Wuhan, China, with accession number CCTCC NO: M 20231757; the taxonomic name of Chlorella N4 is... Chryseobacterium endophyticum N4 was deposited on March 31, 2025, at the China Center for Type Culture Collection (CCTCC), Wuhan University, Wuhan, China, with accession number CCTCC NO: M 2025633. The aforementioned composite microbial suspension promotes nitrogen fixation and nitrogen conversion.

The invention provides a yin-nourishing and lung-moistening fermentation composition and a preparation method thereof. The yin-nourishing and lung-moistening composition with efficient bacteriostatic activity is produced on the basis of a stepped fermentation process. The method comprises the following steps: firstly, carrying out first-stage fermentation on radix ophiopogonis and codonopsis pilosula by utilizing plant lactobacillus MG360; after fermenting for 12 hours, supplementing the extracts of the pears, the liquorice and the loquats and the casei paracasei ID001, and carrying out second-stage fermentation. According to the unique stepped fed-batch fermentation process, the antibacterial effect of the plantaricin EF on pneumonia-related pathogenic bacteria, including streptococcus pneumoniae, beta hemolytic streptococcus, pyogenic streptococcus, escherichia coli, staphylococcus aureus and the like, can be remarkably improved. Meanwhile, the expression of related genes pln-E and pln-F is up-regulated. Animal experiments prove that the composition has excellent effects of nourishing yin, moistening the lung, clearing away the lung-heat and resisting inflammation. The traditional medicinal and edible raw materials are combined with the modern microbial fermentation technology, and a new strategy is provided for developing novel functional products capable of nourishing yin, moistening the lung and resisting infection.

Engineered versions of Streptococcus pyogenes Cas9 (SpCas9) and SpCas9 variants that have improved on-target editing capabilities, and methods of use thereof.

The invention discloses a recombinant uridine diphosphate glucose dehydrogenase mutant and a preparation method thereof, and relates to the technical field of biology, the mutant is derived from pyogenic streptococcus and is obtained through site-specific mutagenesis (mutation site L102V-H129K-I270G), double tags of His-tag and SUMO-tag are fused at the N terminal, the corresponding protein sequence is SEQIDNO.1, and the coding gene sequence is SEQIDNO.2. Compared with a recombinant wild type UGD, the mutant has the advantages that the mutagenesis efficiency is high, and the mutagenesis efficiency is high. The performance of the mutant is remarkably improved, the catalytic activity reaches 1.8 times of that of a wild type, the residual activity is 69.4% after the mutant is stored at room temperature for 5 days, the soluble expression quantity is improved by 5 times, UDPG can be efficiently promoted to be oxidized to generate UDPGA, the commercial production cost of UDPGA is effectively reduced, and the mutant has a wide application prospect. The invention is suitable for the fields of scientific research reagents, biological medicines, cosmetics and the like.

The application discloses a private part care spray capable of balancing private part microecology, removing peculiar smell, killing bacteria and resisting viruses, and belongs to the technical field of private part care and disinfection materials. The private part care spray is prepared by compounding 3.8-6.5 parts of a modified edible bactericide, 3.2-5.5 parts of food-grade lactic acid, 2.2-3.0 parts of modified food-grade gentian root extract and the rest food-grade deionized water. The activity and stability of raw materials are improved through the processes of β-cyclodextrin inclusion modification of the bactericide and compounding of the modified product with the modified food-grade gentian root extract. The product has a killing rate of 99.9% on Neisseria gonorrhoeae, Gardnerella vaginalis, Bacteroides, Streptococcus pyogenes, Candida albicans, Staphylococcus epidermidis, Escherichia coli and Pseudomonas aeruginosa, an inactivation rate of 99.99% on herpes simplex virus SHV-2 and HPV16 virus, an ammonia removal rate of 95%, a peculiar smell inhibition duration of 48 h and a mucous membrane irritation level of 0, and the preparation process is simple and suitable for industrial production.

This invention belongs to the field of probiotic screening and application technology, specifically relating to a strain of *Bifidobacterium longum* subsp. *longum* and probiotic pellets containing this strain, as well as their applications. The *Bifidobacterium longum* subsp. *longum* has the accession number CCTCC NO: M2026130 and can significantly inhibit the growth of *Streptococcus pyogenes*, and can efficiently flocculate with *Streptococcus pyogenes*. The probiotic pellets provided by this invention have strong acid resistance, enteric solubility, and storage stability, and can be widely used for the prevention or treatment of *Streptococcus pyogenes*-induced colonic polyps and colon cancer, showing broad prospects.

This invention pertains to mutant Cas9 nucleic acids and proteins for use in CRISPR / Cas endonuclease systems, and their methods of use. In particular, the invention pertains to an isolated mutant Cas9 protein, wherein the isolated mutant Cas9 protein is active in a CRISPR / Cas endonuclease system, wherein the CRISPR / Cas endonuclease system displays increased on-target editing activity relative to a wild-type CRISPR / Cas endonuclease system. The invention also includes isolated nucleic acids encoding mutant Cas9 proteins, ribonucleoprotein complexes and CRSPR / Cas endonuclease systems having mutant Cas9 proteins that display increased on-target editing activity relative to a wild-type CRISPR / Cas endonuclease system.

The present disclosure relates to targeted genome editing in eukaryotic cells or organisms. More particularly, the present disclosure provides for compositions that may induce modifications in target endogenous nucleic acid sequences in nucleuses of eukaryotic cells. The composition may comprise a single-chain guide RNA (sgRNA) and a Streptococcus pyogenes Cas9 protein. In some embodiments, the sgRNA and the Cas9 protein may be present in a molar ratio ranging from 29:14.0 to 29:1.4.

The application belongs to the field of biological medicine, and particularly relates to application of a fusion protein THSGp in preparation of antibacterial products. The amino acid sequence of the fusion protein THSGp is shown as SEQ ID NO. 1. The fusion protein THSGp can inhibit a plurality of gram-positive bacteria, such as Enterococcus faecalis, Staphylococcus aureus, Listeria monocytogenes and Streptococcus pyogenes. Compared with the Trx-SPRR2A protein with antibacterial activity, the antibacterial activity of the fusion protein THSGp is significantly improved.

The invention relates to a clarithromycin derivative containing a quinolone structure as well as a preparation method and application of the clarithromycin derivative. The compound provided by the invention has antibacterial activity equivalent to that of telithromycin in constitutive streptococcus pneumoniae drug-resistant bacteria and streptococcus pyogenes drug-resistant bacteria. And the antibacterial activity on haemophilus influenzae is better than that of Thailimycin. Furthermore, the anti-streptococcus pyogenes 12-206 activity of part of the compounds disclosed by the invention is obviously superior to that of the existing compounds. In addition, the compound provided by the invention not only can act on ribosome, but also can act on topoisomerase, and is a double-target compound at the cellular level. Therefore, the compound disclosed by the invention is not easy to induce bacteria to generate mutation, and the service life of the compound as an antibacterial agent can be prolonged.