379 results about "Corynebacterium" patented technology

The invention discloses a comprehensive method for extracting L-tryptophan from fermentation liquor. The L-tryptophan is extracted from fermentation liquor which is made from corynebacterium glutmicum as a strain; meanwhile, generated waste liquor is recycled. The comprehensive method comprises the steps of: extracting the L-tryptophan and recycling the waste liquor, wherein the waste liquor generated in an extraction process is treated or recycled for application or used for preparing a compound fertilizer, a feed additive and feed protein. The method completely, effectively and comprehensively utilizes resources, recycles the generated waste liquor while extracting the L-tryptophan, reduces the resource waste, basically realizes the zero emission of waste water, effectively recycles the resources, and lightens the environment-protecting pressure.

The invention discloses a method of producing a protein feed by a liquid-solid two-step fermentation method. The method comprises the following steps: performing liquid culture on corynebacterium glutamicum, candida utilis, bacillus subtilis, lactobacillus plantarum and aspergillus oryzae so as to obtain corynebacterium glutamicum fermentation liquor, candida utilis fermentation liquor, bacillus subtilis fermentation liquor, lactobacillus plantarum fermentation liquor and aspergillus oryzae fermentation liquor; mixing the corynebacterium glutamicum fermentation liquor, the candida utilis fermentation liquor, the bacillus subtilis fermentation liquor, the lactobacillus plantarum fermentation liquor and the aspergillus oryzae fermentation liquor so as to obtain mixed bacterium liquor; loading raw materials of soybean meal, cluster bean meal, cottonseed meal and the like into an explosion vat for performing steam explosion; after steam explosion, mixing materials, the mixed bacterium liquor and molasses in proportion, and adding an enzyme preparation so as to obtain mixed materials; conveying the mixed materials into a special modularized solid-state biological raw material continuous fermentation device for enzymolysis and fermentation, and drying the fermented materials so as to obtain the protein feed. According to the method disclosed by the invention, the modularized automatic fermentation device is adopted, the materials after explosion, the fermented bacterium liquor and the enzyme preparation are mixed, then the mixed materials are loaded into the automatic fermentation device for enzymolysis and biological fermentation, so that requirements for automatic temperature control, automatic dampness control and automatic ventilation control are met, the bioconversion rate is high, the fermentation period is short, the consumption is low, the cost is low, and mechanized and large-scale production requirements can be met.

The invention relates to a recombinant DNA (deoxyribonucleic acid), strain and method for producing L-valine by fermentation. The recombinant DNA comprises DNA sequence for coding acetohydroxy acid synthetase free of feedback inhibition of three branched-chain amino acids to acetohydroxy acid synthetase, DNA sequence for coding dihydroxy reduction isomerase, and DNA sequence for coding branched-chain amino acid aminotransferase. The strain is corynebacterium or brevibacterium transformed by introducing the recombinant DNA. The method is implemented by culturing the strain to produce the L-valine. In the invention, the expression L-valine is used for producing the bacterium L-valine biosynthetic gene, so the yield of the L-valine can be greatly increased; and the fermentation model, especially the high-temperature short-time fermentation model, is constructed according to the optimum temperature of the L-valine biosynthetic enzyme, so that the metabolism intensity of the strain is enhanced, the activity of the L-valine biosynthetic enzyme is enhanced, and the yield of the L-valine and the conversion rate of the sugar acid can be further increased.

The invention discloses a construction method and an application of a corynebacterium gene continuous knockout system, belonging to the technical field of genetic engineering. The corynebacterium gene continuous knockout system comprises a template plasmid and a temperature-sensitive expression plasmid, wherein the template plasmid provides a kan fragment (also known as a kan box) with loxp or variant loxL/R sites at two ends; and the temperature-sensitive expression plasmid carries a Cm resistance gene cat and a temperature-sensitive C. glutamicum replicon, expresses a Cre recombinant enzyme and is used for removing a Km resistance gene kan. The corynebacterium continuous knockout system disclosed by the invention can be generally used for continuous gene transformation of corynebacterium and has the advantages of simplicity and convenience in operation and high efficiency. By utilizing the knockout system, gene (continuous) knockout of three major typical subspecies genomes of the corynebacterium can be successfully completed, and the system is proved to be applicable to research and production of corynebacterium metabolites.

The invention provides a method for producing L-ornithine by microbial fermentation, namely, using Corynebacterium glutamate to obtain the strains of CS-189(cit<(-)>+SG<r>) by chemical treatment, and obtain the L-ornithine hydrochloride by fermentation, culture solution micro-filtration by a ceramic membrane, ion exchange resin and extraction by a hydrothermal crystallization method with decompression concentration. The strains used in the method are auxotrophy resistant to sulfaguanidine, which significantly enhances acid yield by fermentation. Meanwhile, aiming at the problem that the solubility of the L-ornithine hydrochloride in water is extremely difficult, the hydrothermal crystallization method is adopted to obtain better extraction rate under the condition that flammable and explosive alcohol is not used. The method simplifies processes and reduces extraction cost.

The invention relates to the field of microorganisms, in particular to a recombinant strain and application thereof. Experimental results show that before modification, wild corynebacterium glutanmicum ATCC 13869 almost generates no glutamic acid, and the yield of strain MHZ-0112-1 glutamic acid is 4.7 g/L; the yield of MHZ-0112-2 glutamic acid is 18.5 g/L; the yield of strain MHZ-0112-4 glutamic acid and the yield of MHZ-0112-5 glutamic acid are 23.4 g/L and 26.4 g/L respectively. When a strong promoter Psod is independently utilized for expressing a gdh gene, the yield of glutamic acid is increased about 1.8 times; when a promoter Ptuf is independently utilized for expressing a gltA gene, the enzyme activity of CS is improved 4 times, and the yield of glutamic acid is increased to 30.8 g/L; after the two genes are expressed at the same time, the yield of glutamic acid continues to be increased to 35.5 g/L, and the conversion rate reaches about 59%.

The invention discloses a promoter with a nucleotide sequence as follows: 1) a nucleotide sequence shown in sequence 1 in a sequence table; 2) a nucleotide sequence which hybridizes with the nucleotide sequence in 1) under strict conditions and has promoter activity; or 3) a nucleotide sequence which has homology of more than 70% with the nucleotide sequence in 1) and has promoter activity. The promoter provided by the invention can be applied to the biotechnology field with corynebacterium or escherichia bacteria as industrial microbes. Experiments prove a fragment with promoter activity from SEQ ID NO:1 has the activity higher than that of a trc promoter (SEQ ID NO:2).

The invention provides recombinant Corynebacterium glutamicum high in hyaluronic acid yield and a preparation method and application thereof. The recombinant Corynebacterium glutamicum high in hyaluronic acid yield is constructed by genetic engineering technology. The invention also provides application of the recombinant Corynebacterium glutamicum high in hyaluronic acid yield in the fermentative production of hyaluronic acid. The microbial Corynebacterium glutamicum of food safety grade is utilized which never secretes endotoxin, and post-extraction process is simplified; the recombinant Corynebacterium glutamicum high in hyaluronic acid yield constructed by genetic engineering technology can produce hyaluronic acid of different molecular weights (3000 Da to 2000000 Da), the yield of the hyaluronic acid may reach 7 to 20 g/L according to the different molecular weights, and a product may satisfy the needs of foods, cosmetics and pharmaceuticals without complex separation.

The invention discloses an acid-stable glutamic acid decarboxylase (GAD) mutant with pH stability migrating to neutral range, and belongs to the field of biological engineering. The encoding gene of glutamic acid decarboxylase derived from actobacillus plantarum GB01-21 glutamic acid decarboxylase is subjected to site-specific mutagenesis, and the glutamate E on the 89th site is mutated to arginine R or alanine A. The glutamic acid decarboxylase mutant has relative enzyme in pH value of 6.5 increased from the original 38% up to 72% or 84%, and the enzyme activity stability in the neutral pH range is increased significantly. For the transformation with a glutamic acid decarboxylase mutant synthesized by recombinant escherichia coli, the GABA yield reaches 260 g/L, and the yield is 99.6%; and for the transformation with a glutamic acid decarboxylase mutant synthesized by recombinant Corynebacterium glutamicum, the GABA yield reaches 116 g/L, and the yield is 99.5%. The invention reduces the fermentation equipment loss under acidic conditions, and lays foundation for the efficient synthesis of gamma-aminobutyric acid.

The present invention provides Gyrase B and/or Topoisomerase IV par E inhibitors, which can be used as antibacterial agents. Compounds disclosed herein can be used for treating or preventing conditions caused by or contributed by gram positive, gram negative and anaerobic bacteria, more particularly against, for example, Staphylococci, Streptococci, Enterococci, Haemophilus, Pseudomonas spp., Acenetobacter spp., Moraxalla spp., Chlamydia spp., Mycoplasma spp., Legionella spp., Ktycobacterium spp., Helicobacter, Clostridium spp., Bacteroides spp., Corynebacterium, Bacillus spp., Enterobactericeae,’ (E. coli, Klebsiella spp., Proteus spp., etc.) or any combination thereof. Also provided, are processes for preparing compounds disclosed herein, pharmaceutical compositions containing compounds disclosed herein, and methods of treating bacterial infections. (Formula)

The present invention provides ketolide derivatives, which can be used as anti-bacterial agents. Compounds disclosed herein can be used for the treating or preventing conditions caused by or contributed to by gram positive, gram negative or anaerobic bacteria, more particularly against, for example, Staphylococci, Streptococci, Enterococci, Haemophilus, Moraxalla spp., Chlamydia spp., Mycoplasm, Legionella spp., Mycobacterium, Helicobacter, Clostridium, Bacteroides, Corynebacterium, Bacillus, Enterobactericeae or any combination thereof. Also provided are processes for preparing compounds disclosed herein, intermediates used in their synthesis, pharmaceutical compositions thereof, and methods of treating bacterial infections.

The invention discloses recombinant corynebacterium glutamicum for producing gamma-polyglutamic acid as well as a construction method and a use of the recombinant corynebacterium glutamicum. The recombinant corynebacterium glutamicum is characterized by being an engineering bacteria which is obtained by transferring a recombinant expression plasmid containing a gamma-PGA (Poly Glutamic Acid) synthetase composite gene pgsBCA to an original strain wild C.glutamicum ATCC13869. The recombinant corynebacterium glutamicum disclosed by the invention contains the gamma-PGA synthetase composite gene pgsBCA, so that much gamma-PGA can be synthesized under biotin limit or under the fermentation condition of adding Tween40, therefore, the glutamic acid adding cost in the gamma-PGA production process is reduced, and a foundation is laid for the innovation in realizing the one-step production of gamma-PGA better.

The invention belongs to the technical field of bioengineering, and discloses corynebacterium capable of realizing high yield of valine as well as a construction method and an application thereof. Theinvention provides corynebacterium capable of realizing high yield of valine as well as the construction method and the application thereof. According to the corynebacterium capable of realizing highyield of valine, alanine (A) at the first site of a 2-isopropylmalate synthetase gene leuA is mutated into glycine (G). According to the corynebacterium capable of highly producing valine, accumulation of by-product leucine is reduced by weakening a leuA gene, and the production capacity of an L-valine strain is improved. The experiments show that the corynebacterium disclosed by the invention isan L-valine high-yielding strain, can effectively accumulate L-valine and increase the yield of L-valine, lays a foundation for industrial production of L-valine, and has a wide industrial application prospect.

The invention belongs to the field of microorganisms, and particularly relates to a glutamic acid corynebacterium and a construction method and application of the glutamic acid corynebacterium. The glutamic acid corynebacterium has the capability of producing L-lysine, and the activities of pyruvic carboxylase exportin and pyruvic carboxylase in a cell are both strengthened. The glutamic acid corynebacterium strengthens the activity of the pyruvic carboxylase exportin, so that the accumulation of the L-lysine is promoted, the yield of the L-lysine is increased, the activity of pyruvic carboxylase is strengthened at the same time, more precursors are provided for the synthesis of the L-lysine, the accumulation capability of the L-lysine is further improved, the amount of the L-lysine in a culture medium is increased, and the capability of production of the L-lysine through the fermentation of the strain is further improved. The experiment shows that the glutamic acid corynebacterium is a strain with high yield of L-lysine, the L-lysine is effectively accumulated, the yield of the L-lysine is improved, and a foundation is laid for the industrialized production of the L-lysine.

The invention discloses tetrahydropyridine high-yield Corynebacterium glutamicum and application thereof and belongs to the field of bioengineering. Feedback inhibition is relieved through site-directed mutagenesis of Corynebacterium glutamicum aspartokinase gene lysC, and expression of mutational LysC is intensified through promoter replacement. Further, pentose phosphate pathway of host bacteriais further intensified through promoter replacement to meet needs on reducing power NADPH in efficient synthesis of tetrahydropyridine. Finally, tetrahydropyridine high-yield Corynebacterium glutamicum is obtained by transferring tetrahydropyridine synthetic gene cluster ectABC of P. stutzeri into recombinant bacteria. By recombinant Corynebacterium glutamicum, tetrahydropyridine can be efficiently synthesized by utilizing cheap raw materials like glucose and maize plasm; compared with recombinant Escherichia coli produced strains, tetrahydropyridine high-yield Corynebacterium glutamicum hasbetter bio-safety and is of great significance to industrial production and large-scale application of tetrahydropyridine.

The invention relates to fermentation type chopped chilli, which is prepared from fresh red peppers, ginger, garlic, peppers, monosodium glutamate, salt and fermentation broth. A processing technology comprises the following steps: washing the fresh red peppers with bases removed by using 0.5% saline, soaking the red peppers after washing into citric acid solution at 95-98 DEG C for 5 minutes, fishing out, rapidly placing into clear water, thus obtaining the blanched fresh red peppers; mixing saccharomycetes, bacillus aceticus, hydrocarbon corynebacterium and lactic acid bacteria, activating for 3-5min by using hot water at 30-40 DEG C, pouring the bacterium solution after activation into saline, adding rice wine into the saline, thus obtaining the fermentation broth, and regulating the pH of the fermentation broth to 4-5 by using 5% acetic acid solution for later use; and pouring the fermentation broth into the blanched fresh red peppers, adding with the ginger, the garlic, the peppers, the monosodium glutamate and salt, and performing fermentation culture for 10-15h at 20-25 DEG C, and thus obtaining the fermentation type chopped chilli. The fermentation type chopped chilli is unique in flavor and bright red and crispy, and has the shelf life longer than three years.

The invention discloses corynebacterium humireducens and an application thereof. The corynebacterium humireducens is obtained by separating and purifying anolyte of a sludge microbe fuel cell, and is preserved in the ordinary microbe centre of the China Microbe Culture Preservation Management Committee on April 14, 2008 with the preservation number of CGMCC No.2452. The corynebacterium humireducens has wide utilization spectrum of electron donor under the anaerobic condition, has efficient humus reduction capability and electrogenesis capability under the alkaline condition, can be widely applied to in-situ microbe restoration of organic pollutants, microbe fuel cell generating technology or recycling processing of high-concentration organic wastes.

Provided herein are ketolide derivatives, which can be used as antibacterial agents. Compounds described herein can be used for treating or preventing conditions caused by or contributed to by gram positive, gram negative or anaerobic bacteria, more particularly against, for example, Staphylococci, Streptococci, Enterococci, Haemophilus, Moraxalla spp., Chlamydia spp., Mycoplasm, Legionella spp., Mycobacterium, Helicobacter, Clostridium, Bacteroides, Corynebacterium, Bacillus, Enterobactericeae, Propionibacterium acnes or any combination thereof. Also provided are processes for preparation of compounds described herein, pharmaceutical compositions thereof, and methods of treating bacterial infections.

This invention relates to a new mycose synthesized zyme gene clone and its expression in correlate host which zyme gene clone is from corynebacterium glutamicum CICC 10226 which can turn malt sager to mycose under normal biochemical reaction, its substrate conversion efficiency increases along with the reduction of the reaction temperature. It can take malt sager or amylum as the raw material to be turned to 1-mycose with the cloned zyme alpha alpha-1.

A method is disclosed. The method is generally useful for modifying a skin microbiome. The method comprises administering a topical composition to a subject's skin. A topical composition is also disclosed. The topical composition may be used for the method. The topical composition comprises a population of microorganisms, a component obtained from the population of microorganisms, or a combination thereof. The population of microorganisms is generally a Corynebacterium species. The Corynebacterium species comprises at least about 90%, optionally at least about 97%, sequence identity to a 16S rRNA sequence (SEQ ID NO: 1).

The present invention relates to a microorganism producing inosine, which is one of purine nucleoside, an important material for 5′-inosinic acid synthesis, and method for producing inosine using the same. More particularly, the present invention relates to a recombinant microorganism of Corynebacterium genus producing inosine at high concentration by inactivating the gene encoding nucleoside hydrolase II and by enhancing the expression of the gene encoding 5′-nucleotidase, which still retains the characteristics of Corynebacterium ammoniagenes CJIP2401 (KCCM-10610).