89 results about "Ectoine" patented technology

The invention discloses a fermentation process for efficiently synthesizing Ectoine by shortening a fermentation period, which is characterized in that the fermentation process for producing Ectoine by fermenting engineering escherichia coli taking glucose, xylose and arabinose as a mixed carbon source is adopted, so that the fermentation time is shortened to 40.5 h, the yield of Ectoine can reach 112.2 g / L, the production efficiency of Ectoine reaches 2.77 g / L / h, and the yield of Ectoine is greatly improved. And compared with fermentation production of Ectoin by taking glucose as a carbon source, the efficiency is improved by 132%.

The invention relates to the field of bioengineering, in particular to an enzyme composition, a product and application of the enzyme composition in preparation of Ectoine. The invention provides an enzyme composition. The enzyme composition comprises a mutant of 2-hydroxy-4-aminobutyric acid oxidase, a mutant of keto transaminase, a mutant of hydroperoxide hydrolase, a mutant of Ectoine synthetase, a mutant of pyruvic acid-CoA oxidase and lactobacillus helveticus lactic dehydrogenase. According to the method disclosed by the invention, a plurality of mutant enzymes are utilized for combined transformation for the first time, and cheap 2-hydroxy-4-aminobutyric acid is taken as a starting raw material to quickly and efficiently produce Ectoine; the enzyme method has the characteristics of easiness in control, short conversion time, few generated impurities and the like, so that the method has unique advantages in the aspects of low-cost and large-scale mass production of Ectoin.

The invention provides an application of Ectoine or a derivative thereof as a human beta-defensin-2 expression promoter. According to the application, it is found for the first time that Ectoine or the derivative thereof has the effect of inducing cell expression of beta-defensin 2 (hBD-2), so that Ectoine or the derivative thereof is used for improving the defensive mechanism of skin antimicrobial.

The invention discloses an ectoine gene cluster, mutant and application derived from halophilic bacillus, belonging to the field of synthetic biotechnology. The ectoine gene cluster nucleic acid sequence is shown in SEQ ID NO.7, including the following three genes: ectA gene, the nucleic acid sequence is shown in SEQ ID NO.1, ectB gene, the nucleic acid sequence is shown in SEQ ID NO.2, ectC gene, the nucleic acid sequence is shown in SEQ ID NO.3, and the gene cluster is designed and mutated to obtain a mutant, the nucleic acid sequence is shown in SEQ ID NO.8. The invention can improve the synthesis efficiency of ectoine, improve the fermentation content of ectoine, reduce the production cost of ectoine, and is conducive to the popularization and application of ectoine by constructing a recombinant strain using the ectoine gene cluster and its mutant.

The invention provides a nano transdermal sustained-release biological scaffold as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. The nano transdermal sustained-release biological scaffold provided by the invention comprises the following components: a load and a nano carrier, the load comprises mussel mucin, trehalose and Ectoine; the mass ratio of the mussel mucin to the trehalose to the Ectoine is (4 to 6) to (0.5 to 1.5) to (0.5 to 2.0). Through the innovative design of a quaternary composite system of mussel mucin, trehalose, Ectoine and a nano-carrier, the three technical problems of short slow release period, insufficient targeting property and difficulty in large-scale production in a wound repair material in the prior art are successfully solved.

The invention discloses a recombinant halomonas for producing hydroxyectoine as well as a preparation method and application of the recombinant halomonas. The recombinant halomonas disclosed by the invention does not contain an ectoine hydrolase (DoeA) gene, and can be used for expressing ectoine hydroxylase and glutamate dehydrogenase. Experiments prove that when the salt concentration of the recombinant halomonas is reduced to 1.4 M, fermentation can produce 1.02 g / L of hydroxyectoine, and compared with wild halomonas, the yield of hydroxyectoine under the salt concentration of 1.4 M is increased by 10 times. According to the recombinant halomonas disclosed by the invention, the hydroxyectoine is successfully produced under the condition that the salt concentration is reduced, chassis bacteria for efficiently producing the hydroxyectoine under the medium-salt condition are successfully constructed, and theoretical guidance is provided for further reducing the salt concentration for producing the hydroxyectoine; meanwhile, an experimental basis is provided for improving the biosynthesis of a high-added-value chemical hydroxyl ectoine.

The invention relates to the field of agriculture, and in particular to a chitosan functionalized nano-selenium fertilizer and an application thereof. The components of the nano-selenium fertilizer composition and their weight percentages are: 0.5-4.0% chitosan functionalized nano-selenium; 0.8-3.2% ectoine; 0-3.0% alginate; 0.5-5.0% surfactant; and the balance of deionized water. The invention has been found through experiments that in the composition of the invention, ectoine can significantly promote the absorption of chitosan functionalized nano-selenium by plants and promote the enrichment of selenium in plants, and the use of alginate can further increase the promoting effect of ectoine on the absorption and enrichment of nano-selenium by plants, thereby producing a synergistic effect.

The invention discloses a fermentation process for shortening a fermentation period and efficiently synthesizing Ectoine. The fermentation process comprises the following steps: screening and activating high-yield strains, preparing a fermentation culture medium, performing fermentation culture, performing sectional dissolved oxygen regulation and control, performing double-stage pH dynamic regulation and control, performing carbon-nitrogen ratio dynamic regulation based on metabolic flow analysis, performing osmotic pressure gradient induction in the later stage of fermentation, and extracting and purifying a product. According to the fermentation process for shortening the fermentation period and efficiently synthesizing the Ectoin, the expression quantity of key enzymes for synthesizing the Ectoin is increased by 80% or above compared with that of an original strain through the Halomonassp.ECT-09 strain bred through ultraviolet-nitrosoguanidine compound mutation, the basic yield reaches 35 g / L, a foundation is laid for high yield, and the method is suitable for industrial production. A glucose-trehalose mixed carbon source and a yeast extract-ammonium nitrate mixed nitrogen source are adopted, and an L-aspartic acid-L-asparagine mixed precursor is added, so that the metabolic pathway is shortened, and the loss of an intermediate product is reduced.

The invention belongs to the technical field of genetic engineering, and discloses an engineering bacterium capable of producing Ectoine at high yield as well as a preparation method and application of the engineering bacterium. Escherichia coli W3110 is used as a chassis cell, and an Ectoin metabolic pathway is constructed by using synthetic biology. A constitutive promoter Pglgs of a transcription factor related gene in escherichia coli is preferably selected, an ectBAC for coding an Ectoine synthetic gene is preferably selected as a reporter gene, a constitutive plasmid for expressing the Ectoine synthetic gene is constructed, the expression level of the gene is improved, an engineering bacterium Escherichia coli ECT-W020 with high yield of Ectoine is obtained, and the preservation number of the engineering bacterium Escherichia coli ECT-W020 is GDMCC No: 65012. When the engineering bacterium is used for producing Ectoin, the process of adding an inducer is omitted, and the purpose of reducing the production cost is achieved.

The invention belongs to the field of daily chemicals, and discloses a composition with light damage resistance, which comprises a ribonucleic acid solution, tetrahydromethylpyrimidine carboxylic acid and a spirulina maxima extract solution in a weight ratio of (60-80): (10-30): (1-10). The composition takes a ribonucleic acid solution, tetrahydromethylpyrimidinecarboxylic acid and a spirulina maxima extract solution as core components, can play a better anti-light damage effect, and samples are sent to a third-party detection mechanism for zebrafish embryo light damage cell apoptosis test. The test result shows that the effect of the composition is better than the cell apoptosis effect of ergothioneine with the same concentration, and evaluation of the skin moisture content increasing effect shows that the composition can well repair and supplement the skin barrier; meanwhile, the invention further discloses application of the composition and cosmetics.

The application discloses a nisin protective agent containing ectoine and application, and belongs to the technical field of nisin storage and application. The nisin protective agent containing ectoine comprises ectoine, pullulan and 2-hydroxybutanedioic acid in a mass percentage ratio of 20-70%, 10-50% and 10-40% respectively. The preparation method comprises the following steps: ectoine, pullulan and 2-hydroxybutanedioic acid are weighed according to the mass percentage ratio, uniformly mixed in a mixer, and then ground in a grinder at 20-30 DEG C to a particle size of 100-200 nm, so that the nisin protective agent containing ectoine with uniform quality is obtained. The application can significantly improve the stability of nisin in the storage and application process.

The invention discloses a streptomycete recombinant bacterium for enhancing the synthesis capability of ectoine and hydroxyectoine and application of the streptomycete recombinant bacterium. According to the invention, streptomyces SCUT-1 is taken as a starting strain, and an efficient promoter scutP1 is utilized to carry out overexpression on a coding gene for synthesizing ectoine and a coding gene for synthesizing hydroxyl ectoine. Compared with the original strain streptomyces SCUT-1, the tetrahydropyrimidine and hydroxyl tetrahydropyrimidine synthesis capability and hyperosmosis resistance of the recombinant strain are obviously improved, the degradation efficiency of the recombinant strain is obviously enhanced, and waste feathers, cottonseed meal and shrimp shells can be directly used as the unique carbon and nitrogen source for fermentation; and the yields of the recovered highest soluble amino acid and polypeptide are respectively 2.15, 1.18 and 1.15 times of those of the original strain. The method has great significance in high-value conversion of agricultural wastes, does not need to additionally add a reducing agent, has mild reaction conditions, and has important values for reducing the production cost and improving the yield.

The present invention belongs to the technical field of biological medicine, and discloses a ceramide compound shown in Chemical Formula I: #imgabs0# wherein, R 1 is selected from the residues after condensation of sialic acid, chelidamic acid monohydrate, 2,5-furandicarboxylic acid, quinic acid, chlorogenic acid, ectoine, nicotinic acid, shikimic acid, glucuronic acid, salicylic acid, carnosic acid, dihydrooat acylanthranilic acid, abietic acid, ursolic acid, tranexamic acid, syringic acid, gentisic acid, vanillic acid, 5-hydroxyisovanillic acid, orotic acid, 5-aminoorotic acid, 3,5-dihydroxy-4-methylbenzoic acid, glycyrrhizic acid or gallic acid, R 2 is selected from one of the following structures: -C 15 H 29 、-C 15 H 31 、-C 15 H 27 、-CHOHC 14 H 27 、-CHOHC 14 H 29 . The present invention also discloses a preparation method and application of the ceramide compound. The present invention introduces a molecular fragment with physiological activity of a functional carboxylic acid into ceramide, which will enhance the original efficacy of this type of molecule and also improve the physicochemical properties of the ceramide compound.

This invention discloses a coconut oil antibacterial and deodorizing essence, its preparation method, and its application. The essence consists of: 12-50% coconut oil, 4-10% butylene glycol, 0.1-2% sodium hyaluronate, 1-5% panthenol, 2-5% lactic acid, 2-5% potassium lactate, 1.5-6% emulsifier, 0.1-0.6% 4-tert-butylcyclohexanol, 0.1-6% ectoine, 0.1-6% ergothioneine, 1-2% non-irritating preservative composition, and the remainder being pure water. The components work synergistically. Disposable sanitary products using this essence can inhibit bacteria and deodorize, soften the skin, relieve itching, improve redness, and soothe sensitivity. Washing and care products using this essence have good inhibitory effects on harmful bacteria, build a natural biological protective film on the skin surface, and have anti-inflammatory, soothing, repairing, anti-itch, and redness-reducing properties, leaving the skin moisturized and firm.

The method provides a skin barrier repairing composition derived from an extreme environment, combines Antarctic chlorella extract, ectoine and glycerol glucoside, the composition has a significant synergistic effect in repairing the skin barrier, and the cosmetic applying the composition has excellent repairing effect, fundamentally solves a series of problems of the skin, and has a good application prospect.

This invention provides an engineered bacterium for producing ectoine, its preparation method, and its application. The engineered bacterium is prepared using a method comprising the following steps: using *Corynebacterium glutamicum* as the starting strain, feedback inhibition by aspartate kinase LysC is relieved, the activity of the gene lysE encoding lysine efflux permease is reduced, and the ectoine synthesis gene cluster ectABC is expressed. Based on this, the activity of any one or at least two of the following—diaminobutyrate acetyltransferase EctA, pyruvate carboxylase Pyc, or aspartate kinase LysC—is enhanced, and / or the activity of any one or at least two of the following—maloquinone oxidoreductase Mqo or homoserine dehydrogenase Hom—is reduced to further increase ectoine yield.

The invention relates to a genetically engineered bacterium for efficiently synthesizing ectoine and a construction method of the genetically engineered bacterium. The genetically engineered bacterium comprises a related protein degradation tag, an expression vector and a construction method of the expression vector. According to the invention, a protein degradation tag is added to regulate and control a diaminopimelate decarboxylase gene lysA and a homoserine dehydrogenase gene metL, and meanwhile, an exogenous gene cluster ectABC is introduced to construct an expression path of ectoine in a host cell so as to realize de novo synthesis of ectoine. By means of the construction method, the content of essential amino acid needed by life metabolism of thalli is guaranteed, growth of the strains is promoted and affected, and the purpose of high yield of ectoine is achieved. The amino acid sequence of the protein degradation tag is mutated on the basis of regulation and control of the protein degradation tag, and it is found that the mutated protein degradation tag is more beneficial for increasing the yield of ectoine.

The invention relates to an expression vector which comprises an aspartate dehydrogenase gene segment, a glutamate dehydrogenase gene segment and a glutamate synthase gene segment. The expression vector can regulate and control the metabolic pathway of glutamic acid in host cells and increase the accumulation of glutamic acid in the host cells, so that the production capacity of ectoine in the cells is further influenced. The invention also provides a genetically engineered bacterium, through a plasmid or genome integration method, the gene cluster ectABC is overexpressed, the aspartate dehydrogenase gene is overexpressed, the glutamate dehydrogenase gene is overexpressed, the glutamate synthase gene is overexpressed, and the glutamine ligase gene is weakly expressed, so that the recombinant engineering bacterium for high-yield production of ectoine is obtained. According to the invention, the content of glutamic acid in cells is increased by enhancing the expression of the aspartate dehydrogenase gene, the glutamate dehydrogenase gene and the glutamate synthase gene, and the metabolism of glutamic acid to glutamine is reduced by weakening the glutamine ligase gene, so that the synthesis yield of ectoine is increased; and glutamic acid required by growth of host cells can be guaranteed.

The invention provides a method for producing Ectoine through salt impact-glutamine synergistic feeding, and relates to the field of fermentation engineering. The invention particularly relates to a metabolism regulation strategy of salt impact in cooperation with glutamine supplement. According to the method, after salt impact is applied in the logarithmic phase of halophilic bacteria, glutamine is accurately supplemented to activate a thallus stress synthesis pathway. Through the method, the yield of Ectoin can be remarkably increased, the fermentation period is shortened, and the metabolic activity of thalli is enhanced. The method is simple and convenient to operate and controllable in cost, and an innovative process solution is provided for efficient production of industrial fermentation of the halophilic bacteria.

The invention discloses ectoine fermentation liquor and application thereof in growth promotion and salt resistance of rice. The ectoine fermentation liquor is efficiently synthesized by using a low-quality carbon source, the yield and fermentation efficiency of ectoine are improved while the cost is saved, the yield of ectoine can reach 134 g / L, and the production rate of ectoine reaches 3.73 g / L / h. Furthermore, an application technology of unpurified fermentation liquor in rice seedling growth promotion and salt stress resistance is developed. The ectoine fermentation liquor can effectively promote the growth of the rice and the tolerance of the rice to saline-alkali soil. The invention provides an economical and feasible technical scheme for a low-inferior carbon source fermentation process of ectoine and the field of agricultural application of ectoine.

This invention discloses a repair composition containing multiple ceramides and its preparation method, belonging to the field of cosmetic technology. The repair composition, by mass parts, comprises ceramide AP, ceramide EOP, ceramide NP, phytosphingosine, Centella asiatica extract, Portulaca oleracea extract, Hydrangea macrophylla extract, ectoine, and tripeptide-1. This invention utilizes multiple ceramides and phytosphingosine to biomimetically replenish the lipids of the stratum corneum, combined with the synergistic effects of various plant active ingredients and active peptides, to effectively achieve multiple effects such as skin barrier repair, soothing and anti-inflammatory properties, and anti-allergy. This invention also provides a barrier repair serum containing this repair composition and its preparation method. The serum has stable physicochemical properties and, verified by in vitro cell experiments and human efficacy tests, can rapidly repair damaged skin barriers, improve sensitive symptoms such as redness and stinging, and is gentle and non-irritating, suitable for daily skincare use by people with sensitive skin and damaged skin barriers.

The present invention relates to a process for the extraction and purification of ectoine from a fermentation broth, comprising at least the successive steps of: a) Passing said fermentation broth through a cation exchange resin able to capture ectoine, and eluting ectoine, to obtain an ectoine aqueous solution; b) Concentrating said ectoine aqueous solution and then drying it, to obtain raw crystals of ectoine; c) Dissolving said raw crystals of ectoine into hot methanol; d) Hot filtrating said methanol-ectoine mixture; e) Crystallizing ectoine by cooling of the filtered methanol-ectoine mixture, and recovering high purity ectoine crystals.

The invention belongs to the technical field of biomedical materials, and particularly relates to an antioxidant for improving the stability of mussel mucin and application of the antioxidant. The antioxidant provided by the invention is a combination of an antioxidant A and an antioxidant B, or a combination of the antioxidant A, the antioxidant B and an antioxidant C. The antioxidant A is composed of Ectoine, mussel mucin type 6 (Mfp-6), glycerin and polyvinylpyrrolidone (PVP), the antioxidant B is an antioxidant containing reduced sulfur, and the antioxidant C is a hydrogen supply type antioxidant. The mussel mucin is protected by the components in the antioxidant through multiple mechanisms, so that the oxidation degree and the oxidation rate of the mussel mucin in the mussel mucin preparation are obviously slowed down.

The invention relates to the field of microbial fermentation engineering, and particularly discloses a stirring paddle combination method and system for optimizing fermentation of halophilic bacteria to produce Ectoine. Through CFD simulation and comparison of eight three-layer paddle combinations, it is found that a CD6-RT-RT combination with a semicircular tube paddle (CD6) arranged on the bottom layer and six flat blade paddles (RTs) arranged on the middle layer and the top layer can synergistically improve the gas-liquid mass transfer efficiency of a high-salt system, bubbles are broken through strong radial flow of the CD6 paddle on the top layer, and a gas-liquid interface is expanded in combination with high-shear axial flow of the RT paddles on the middle layer and the bottom layer; uniform distribution of the gas content (gradient is less than or equal to 5%) and kLegt are realized; compared with a traditional combination, the efficiency is improved by 25%-72%; in a 50L tank, the yield of Ectocaine reaches 32.2 g / L, which is obviously improved by 15.0% compared with that of a conventional RT-RT-RT combination. According to the method, the oxygen transfer bottleneck of high-salt fermentation is improved, and an efficient and low-consumption scheme is provided for industrial production of Ectoin.

The invention discloses a construction method and application of an ectoine-producing genetically engineered bacterium, and belongs to the field of biosynthesis. Escherichia coli BL21 is used as an original strain, lacI genes of the Escherichia coli BL21 are knocked out, an expression system regulated and controlled by tryptophan is constructed, and synthesis of a product ectoine can be regulated and controlled under the condition that an inducer is not added. According to the invention, a phosphotransferase system of Escherichia coli BL21 is modified, and a glucose readifier protein coding gene Zmglf is integrated at a ptsG gene locus, so that the inhibition effect of carbon catabolism is relieved, and the cost of a culture medium is reduced. Through overexpression of a phosphoenolpyruvate carboxylase coding gene ppc and an aspartate semialdehyde dehydrogenase coding gene asd, the accumulation of precursor substances oxaloacetic acid and L-aspartate-beta-semialdehyde in the growth and metabolism process is enhanced, and the synthesis level of the product ectoine is improved.

The invention discloses a preparation method of a sodium hyaluronate Ectoine fermentation product, which comprises the following steps: lactic acid bacteria are inoculated into a fermentation substrate, and fermentation culture and sterilization are performed to obtain the sodium hyaluronate Ectoine fermentation product, the fermentation substrate is composed of sodium hyaluronate, Ectoine and water; the lactic acid bacteria consist of lactobacillus mucus fermentation and lactobacillus helveticus. Sodium hyaluronate and Ectoine are fermented through lactic acid bacteria, a synergistic effect can be achieved, and the prepared product can be directly used as a cosmetic and can also be added into a basic formula of the cosmetic for use, has good tightening and relieving effects, is basically free of irritation and has a good application prospect in the field of cosmetics.

This invention relates to the field of separation and purification technology, and in particular to a method for separating and purifying ectoine from fermentation broth. This invention provides an integrated purification process combining aqueous two-phase extraction technology with antisolvent-temperature coupled crystallization technology, which can solve the problems of poor selectivity in separating ectoine from impurities and difficulty in controlling the crystallization process, ultimately achieving a balance between high yield (≥95%) and high purity (≥99.5%).

The invention discloses an eye protection external preparation and a preparation method thereof, and belongs to the technical field of ophthalmic preparations. The preparation method comprises the following steps: mixing a main agent solution A containing Ectoine and trehalose with a matrigel solution B containing chitosan quaternary ammonium salt; and then carrying out high-pressure microjet homogenization treatment on the mixed solution at 100-150 MPa so as to construct a supramolecular network structure. Through a specific high-pressure homogenization process, the obtained preparation has an excellent shear thinning characteristic, high viscosity is kept during standing to realize biological adhesion, the viscosity is instantaneously reduced during blinking to ensure comfort, and the problem of blurred vision of traditional gel is solved. Meanwhile, due to the network structure of the preparation, the active ingredients can be instantly released, the hypertonic state of the eyes can be rapidly relieved, and the preparation is adhered to the ocular surface for a long time.

The invention discloses a low-temperature-resistant biostimulant composition screened based on a CBFs gene. The low-temperature-resistant biostimulant composition comprises beta-nicotinamide mononucleotide and at least one of Ectoine and / or a microbial fermentation product containing Ectoine, amino acid and a derivative thereof, sugar and a derivative thereof, and a seaweed extract. The biostimulant composition contains beta-nicotinamide mononucleotide, so that the low-temperature-resistant CBFs gene expression in plants can be promoted, the low-temperature stress resistance of the plants is improved, the plant growth is promoted, and the yield is increased.

The present invention relates to a liquid vaccine composition comprising a live virus and a natural deep eutectic solvent (NADES) as a carrier, which further comprises an additive selected from methionine and (hydroxy)ectoine, which additive is capable of reducing the loss of virus titer over time upon storage in NADES-based liquid vaccine compositions containing up to 50% water by weight. Such compositions have a relatively low viscosity which is favorable for the manufacture of the carrier and for the formulation and use of liquid vaccines.