79 results about "Ectoine" patented technology

The object of the present invention is to provide a method for producing ectoine and hydroxy ecto by cultivating halophilic microorganisms with reasonable cost to improve the synthesis efficiency of ectoine and hydroxy ecto. The method for cultivating halophilic microorganisms of the present invention to produce hydroxyectoine comprises the following steps: (1) strain activation; (2) seed cultivation; (3) fermentation stage; (4) reducing the salt concentration of the culture medium by feeding Rapidly increase to 3-3.5M, and then continue to cultivate for 3-8h, so that the synthesis of ectoine is greatly increased; (5) raise the culture temperature from the optimal growth temperature to a higher temperature, continue to cultivate for 3-10h, and obtain a higher High yield of ectoine synthesis. In the invention, the fermentation inoculation strain is cultivated by means of osmotic pressure shock and heat shock in the fermentation process, and the synthesis rate of ectoine and hydroxy ectoine is greatly improved at a reasonable cost.

The invention relates to a method for fermenting and generating Ectoine by utilizing microorganisms, which belongs to the microorganism fermentation engineering filed. The method comprises the following steps: fermenting a plurality of strains of Halomonas, which can secrete Ectoine, in a liquid culture medium with glutamic acid mono sodium as a sole carbon nitrogen source and the NaCl concentration being 15 to 120g/L; inducing synthesis and enaling Ectoine to be secreted; and recycling the Ectoine in a fermentation system. The method ensures that the fermentation productivity of the Ectoin reaches 6.4 to 7.9g/L/d, which is 2.1 to 2.4 times of that of the prior art, and products are mostly secreted into the culture medium, so as to simplify the recycling process of the products, and to be favor of realizing continuous culture. Compared with the prior art, the NaCl concentration in the culture medium is reduced by 1/2 to 2/3, thus being favor of reducing the corrosion of equipment, and reducing the downstream processing cost.

The invention relates to the field of daily chemical products, in particular to a three-dimensional moisture preservation factor. The three-dimensional moisture preservation factor is prepared from the following ingredients in parts by weight: 30 parts of saccharide isomerate, 5 to 12 parts of Ectoin, 1 to 5 parts of malt extracts, 0.1 to 1.5 parts of decoyl hydroximic acid, 0.1 to 0.4 part of 1,2hexanediol, 0.1 to 0.5 part of sodium polyglutamate, 0.01 to 0.5 part of hyaluronic acid, 0.01 to 0.1 part of sodium hyaluronate and 68 to 80 parts of water.

The invention provides an eye care composition. The eye care composition is prepared from chondroitin sulfate, hyaluronic acid and ectoin or an ophthalmologically acceptable ectoin derivative. In theeye care composition, the chondroitin sulfate accounts for 0.05 to 2 percent by mass preferably 0.1%-1%; the mass percentage of the hyaluronic acid is 0.01%-2.5%, preferably 0.05%-1.5%; and the masspercentage of the ectoine or the ophthalmologically acceptable ectoine derivative is 0.5%-5%, preferably 0.5%-2%. Compared with the prior art, according to the eye care composition disclosed by the invention, hyaluronic acid, ectoin and chondroitin sulfate are combined according to a certain weight ratio, so that the repair of corneal injury can be accelerated; the anti-fatigue effect is remarkable, the recovery time of iris congestion and swelling is short, and the growth of escherichia coli, staphylococcus aureus, pseudomonas aeruginosa and candida albicans is well inhibited. More importantly, the irritation of chondroitin sulfate can be obviously relieved.

The invention provides a lactobacillus freeze drying protective agent, which comprises 0.1-4% of ectoine, 2-8% of mycose, 10-20% of skimmed milk and 0.1-2% of monosodium glutamate. The protective agent can greatly increase the live bacteria viability amount of lactobacillus during a freeze-drying processing process. The agent has the characteristics of convenient usage, obvious effect, no pollution and environment protection. By using the freeze-drying process for processing lactobacillus by the protective agent, the lactobacillus freeze-drying powder live bacteria amount can reach more than 1*1011CFU/g.

The invention discloses an ectoin-contained soothing and repairing composition. The soothing and repairing composition comprises ectoin, panthenol, sodium hyaluronate and polyol. Since the ectoin, thesodium hyaluronate and the panthenol can be compounded, the soothing and repairing composition can more effectively improve the immune protection ability of skin cells and improve the cell repairingability, so that skin can effectively resist invasion of microbes and allergens, and for conditions of skin peeling, reddening, fever and the like, a good repairing effect can be exerted, and recoveryof skin health is accelerated. The soothing and repairing composition can also alleviate skin damage caused by ultraviolet rays, skin aging caused by a dry environment and skin deterioration caused by a surface active agent. Moreover, the soothing and repairing composition is mild and is free of irritation, and can be applied to various cosmetics as a soothing and repairing active component.

The invention discloses a construction method of a high-yield ectoine engineering strain and an application thereof, and belongs to the technical field of bioengineering. The present invention provides an E.coli BL21 (DE3) recombinant bacterium capable of producing ectoine under low salt conditions, the bacterium comprising exogenous genes ectA, ectB and ectC having a specific RBS sequence controlled by a T7 promoter, and post-transcriptional level inhibition of ptsG, pta, thrA and lysA genes using a sRNA technology. The recombinant escherichia coli constructed by the invention takes glucose as a substrate, and after fermentation is carried out for 72 hours, the tetrahydropyrimidine yield can reach 30g/L.

The invention discloses novel application for Ectoine, namely application of the Ectoine in preparing medicines of treating arthritis. The effective dosage range of the Ectoine is 0.1 to 100 mg/kg of body weight, preferably 8 to 50mg/kg of the body weight. When in use, the Ectoine can be used independently, or can be combined with other medicines for use. The Ectoine has high water solubility and osmoregulation function; and the experiment proves that the Ectoine can stabilize joint tissue and joint synovia, improve the adaptability of the joint tissue to environment change and improve the curative effect of other components in a preparation except for the Ectoine, so the Ectoine can be used for treating the arthritis.

The invention discloses a cross-linked hyaluronic acid and ectoin isotonic wound irrigating fluid. The irrigating fluid comprises cross-linked hyaluronic acid dialysate and ectoin; and the cross-linked hyaluronic acid dialysate is prepared by steps as follows: hyaluronic acid or hyaluronate is subjected to a cross-linking reaction with a cross-linking agent in an alkaline water environment, and the obtained reaction solution is dialyzed and purified by a phosphate buffer solution until osmotic pressure is 280-350 mOsmol/kg after pH value is regulated by acid. The osmotic pressure of the woundirrigating fluid is 280-350 mOsmol/kg, while the wound is cleaned, an active factor ectoin accelerates self-healing of injured cells due to capacity of improving immunity of skin cells, cross-linked hyaluronic acid attaching to the wound can continuously plays the function of autolytic debridement and is gathered on the wound surface to form a hyaluronic acid protecting membrane, and functions ofstopping bleeding, preventing tissue adhesion, resisting bacteria, protecting the wound and promoting healing are realized.

The invention provides a high-performance liquid detection and content determination method for ectoine. Chromatographic conditions are as follows: the chromatographic column adopts an adamantine-bonded chromatographic column; the detector adopts a DAD detector; the mobile phase comprises a mobile phase A and a mobile phase B; the mobile phase A is methanol; the mobile phase B is an aqueous buffersolution of monopotassium phosphate; and the elution mode adopts isocratic elution. By utilizing the chromatographic conditions, a test solution and a standard solution are injected into a liquid chromatograph, the chromatogram is recorded, the peak area is calculated according to an external standard method, and the content of ectoine in the sample can be accurately determined. The method disclosed by the invention is short in detection time, excellent in separation effect, high in precision and accuracy and low in cost and has an important application value in aspects of strain screening and related quantitative researches during ectoine production.

The invention discloses a culture medium and fermentation method capable of improving yield of corynebacterium glutamicum ectoines, and belongs to the field of bioengineering. The invention provides aformula of a fermentation culture medium. The fermentation culture medium is rich in a carbon source, a nitrogen source, inorganic salt and compound trace elements, which are required for growth of corynebacterium glutamicum CG-ECT3; a strain is enabled to rapidly grow, reproduce and be cultured for 13 hours; OD600 can reach over 40; a plasmid copy number is increased; and time of a lag period and time of a logarithmic phase are shortened. Meanwhile, according to the invention, by controlling residual sugar and a precusor substance L-asparaginic acid in fermentation liquor to be replenished in the fermenting process, the acid-producing ability of the recombinant corynebacterium glutamicum CG-ECT3 is improved, a fermentation period is shortened, and after fermentation is carried out for 45h, yield of the ectoines can reach over 48g/L.

The invention relates to a method for increasing activity of a microbial flocculant, which is characterized in that Ectoine or tetrahydropyrimidine is added, the stable conformation of the microbial flocculant is protected, and the flocculant activity can be enhanced. The method provided by the invention solves the problem that when the microbial flocculant, is used for treating waste water with high ion concentration, high concentration heavy metal, extreme pH, and high/low temperature, the flocculation activity of the microbial flocculant is obviously reduced. The method for increasing activity of the microbial flocculant has application prospect on treatment of water and waste water, fermentation and food postprocessing.

The invention discloses a saccharomyces cerevisiae engineering strain for synthesizing ectoine through fermentation, and belongs to the technical field of biological engineering. According to the invention, saccharomyces cerevisiae is taken as a host, a triple shuttle plasmid pEBS of bacillus subtilis, escherichia coli and saccharomyces cerevisiae is taken as an expression vector, an ectABC gene cluster derived from halomonas elongata is introduced into the saccharomyces cerevisiae, then strategies such as ectA, ectB and ectC single-gene RBS optimization, promoter optimization, single-gene RBS and promoter double optimization and the like are carried out, and efficient biosynthesis of ectoine is realized. The ectoine accumulation amount is up to 2.3 g/L when the recombinant saccharomyces cerevisiae strain constructed by the invention is cultured for 96 hours on a shake flask, and the recombinant saccharomyces cerevisiae strain has potential and wide application value in industry.

The invention discloses a method for constructing and optimizing an engineering strain for producing ectoine, and belongs to the technical field of biological engineering. The invention provides an Escherichia coli engineering bacterium ECT-LA capable of producing ectoine under a low-salt condition. The bacterium is based on escherichia coli E.coli BL21 (DE3) and comprises an ectoine synthetic gene cluster ectABC controlled by a T7 promoter and exogenous genes lysCC932T and asd; a preparation method of ectoine is optimized futher. The recombinant escherichia coli constructed by the invention takes glucose as a substrate, and the yield of ectoine can reach 60g/L after fed-batch fermentation is performed for 56h.

The invention discloses a lactic acid bacteria freeze-drying protecting agent and application thereof. The protecting agent is composed of a skimmed milk powder solution and an ectoine solution, content of skimmed milk powder is 5-30wt% of the protecting agent, and content of ectoine is 0.08-0.5wt% of the protecting agent. Different from conventional protecting agents having complex formulas, the freeze-drying protecting agent is simple in formula composition, and bacteria activity after freeze-drying is higher than that of other commonly-used protecting agents, so that viable count is guarantee. Experimental study finds that the freeze-drying protecting agent plays an unexpected role in protecting lactic acid bacteria, the lactic acid bacteria after being mixed with the protecting agent are freeze-dried, obtained bacterial powder is high in activity and stability, and freeze-drying has little impact on the lactic acid bacteria, so that long-time storage and transportation of the bacterial powder are facilitated.

The invention discloses an oral ulcer film with a repairing effect, and relates to the field of oral ulcer repairing. The oral ulcer film with the repairing effect comprises the following raw materials of a film-forming agent, a humectant and functional substances, wherein the functional substances are hyaluronic acid, ectoine and ergothioneine with anti-inflammatory, bactericidal and repairing effects. According to the oral ulcer film, a preparation process is simple, the cost is low, the film-forming property is good, the safe and effective effects are achieved, the raw materials are easy toobtain, and the large-scale production can be achieved; and compared with a traditional oral ulcer film, the oral ulcer film containing the functional substances does not contain any medicinal substances, has no irritating effect on an oral ulcer surface, is simple, safe and free of toxic and side effects in components, can be attached to the ulcer surface, has the effects of bacteria blocking, inflammation resistance and repairing, and can effectively promote ulcer healing, repair and protect wounds, and prevent infection.

The invention discloses a ectoine-containing special foliar fertilizer for saline-alkali soil as well as a preparation method and application of the foliar fertilizer. The foliar fertilizer is prepared by taking ectoine as a main effective component, taking ectoine and gamma-aminobutyric acid as stress resistance factors and mannitol and monopotassium phosphate as fertilizer synergists, is used for foliar spraying in a seedling stage and a mature stage of saline-alkali soil crops, and is verified by indoor pot culture experiments and field plot experiments. The problems that crops are not easyto survive and grow slowly in saline-alkali soil are solved, the content of free proline in the crops and the expression of an antioxidant enzyme system can be remarkably improved, and the yield increase of the crops and the improvement of the quality of the crops are promoted.

The invention discloses a diaminobutyric acid acetyltransferase mutant as shown in SEQ ID NO: 3, the diaminobutyric acid acetyltransferase mutant can efficiently catalyze the reaction of L-2, 4-diaminobutyric acid to generate N-acetyl-2, 4-diaminobutyric acid, and the N-acetyl-2, 4-diaminobutyric acid is further catalyzed by ectC enzyme to generate Ectoine.

The invention relates to a composition for resisting allergy and repairing. The composition is prepared from the following components: asiaticoside, Ectoin, a herba portulacae extract, a convalescent tomentosa extract, butanediol and pure water. The method comprises the following steps: heating pure water and keeping the temperature constant; the preparation method comprises the following steps: adding the asiaticoside and the convalescent tomentosa extract into pure water, and uniformly stirring to obtain a mixture I; adding the purslane extract and Ectoine into the mixture I, and uniformly stirring to obtain a mixture II; adding butanediol into the mixture II, uniformly stirring, and filtering to obtain the composition for resisting allergy and repairing. The tomentosa extract and the centella asiatica total glycosides can effectively relieve irritation to skin, reduce irritation of external substances to the skin, enable the skin to be in an optimal state at any time, efficiently block external irritation and also have a repairing effect on sensitive skin.

The invention discloses a construction method and application of recombinant bacteria for producing ectoine. Coding genes of diaminobutyric acid aminotransferase, diaminobutyric acid acetyltransferase and ectoine synthetase are introduced into mutant escherichia coli through a recombinant vector to construct the recombinant bacteria for producing ectoine. By using the recombinant bacteria, the branching pathway of L-aspartate semialdehyde can be blocked, and the yield of ectoine and the conversion efficiency of aspartate are effectively improved;the recombinant strain constructed by the method disclosed by the invention has the advantages of cheap raw materials, simple process, high production efficiency and the like when being used for producing ectoine, and has a good industrial application prospect.

The invention aims to provide a PCR synergistic additive Ectoine. The Ectoine can be used to enhance PCR amplification effect and especially has a remarkable effect of amplifying super-long DNA fragments.

The invention discloses a method for constructing an Ectoine producing strain, the constructed genetically engineered strain CGMCC No.22733 can directly produce Ectoine through fermentation by taking glucose as a carbon source, 42.7 g/L of Ectoine can be produced through fermentation in a 5L fermentation tank for 48 hours, and the Ectoine producing strain has development and application potential.