55 results about "Ectoine" patented technology

The invention provides a nano transdermal sustained-release biological scaffold as well as a preparation method and application thereof, and belongs to the technical field of biomedical materials. The nano transdermal sustained-release biological scaffold provided by the invention comprises the following components: a load and a nano carrier, the load comprises mussel mucin, trehalose and Ectoine; the mass ratio of the mussel mucin to the trehalose to the Ectoine is (4 to 6) to (0.5 to 1.5) to (0.5 to 2.0). Through the innovative design of a quaternary composite system of mussel mucin, trehalose, Ectoine and a nano-carrier, the three technical problems of short slow release period, insufficient targeting property and difficulty in large-scale production in a wound repair material in the prior art are successfully solved.

The invention discloses a fermentation process for shortening a fermentation period and efficiently synthesizing Ectoine. The fermentation process comprises the following steps: screening and activating high-yield strains, preparing a fermentation culture medium, performing fermentation culture, performing sectional dissolved oxygen regulation and control, performing double-stage pH dynamic regulation and control, performing carbon-nitrogen ratio dynamic regulation based on metabolic flow analysis, performing osmotic pressure gradient induction in the later stage of fermentation, and extracting and purifying a product. According to the fermentation process for shortening the fermentation period and efficiently synthesizing the Ectoin, the expression quantity of key enzymes for synthesizing the Ectoin is increased by 80% or above compared with that of an original strain through the Halomonassp.ECT-09 strain bred through ultraviolet-nitrosoguanidine compound mutation, the basic yield reaches 35 g / L, a foundation is laid for high yield, and the method is suitable for industrial production. A glucose-trehalose mixed carbon source and a yeast extract-ammonium nitrate mixed nitrogen source are adopted, and an L-aspartic acid-L-asparagine mixed precursor is added, so that the metabolic pathway is shortened, and the loss of an intermediate product is reduced.

The invention belongs to the field of daily chemicals, and discloses a composition with light damage resistance, which comprises a ribonucleic acid solution, tetrahydromethylpyrimidine carboxylic acid and a spirulina maxima extract solution in a weight ratio of (60-80): (10-30): (1-10). The composition takes a ribonucleic acid solution, tetrahydromethylpyrimidinecarboxylic acid and a spirulina maxima extract solution as core components, can play a better anti-light damage effect, and samples are sent to a third-party detection mechanism for zebrafish embryo light damage cell apoptosis test. The test result shows that the effect of the composition is better than the cell apoptosis effect of ergothioneine with the same concentration, and evaluation of the skin moisture content increasing effect shows that the composition can well repair and supplement the skin barrier; meanwhile, the invention further discloses application of the composition and cosmetics.

The application discloses a nisin protective agent containing ectoine and application, and belongs to the technical field of nisin storage and application. The nisin protective agent containing ectoine comprises ectoine, pullulan and 2-hydroxybutanedioic acid in a mass percentage ratio of 20-70%, 10-50% and 10-40% respectively. The preparation method comprises the following steps: ectoine, pullulan and 2-hydroxybutanedioic acid are weighed according to the mass percentage ratio, uniformly mixed in a mixer, and then ground in a grinder at 20-30 DEG C to a particle size of 100-200 nm, so that the nisin protective agent containing ectoine with uniform quality is obtained. The application can significantly improve the stability of nisin in the storage and application process.

This invention discloses a coconut oil antibacterial and deodorizing essence, its preparation method, and its application. The essence consists of: 12-50% coconut oil, 4-10% butylene glycol, 0.1-2% sodium hyaluronate, 1-5% panthenol, 2-5% lactic acid, 2-5% potassium lactate, 1.5-6% emulsifier, 0.1-0.6% 4-tert-butylcyclohexanol, 0.1-6% ectoine, 0.1-6% ergothioneine, 1-2% non-irritating preservative composition, and the remainder being pure water. The components work synergistically. Disposable sanitary products using this essence can inhibit bacteria and deodorize, soften the skin, relieve itching, improve redness, and soothe sensitivity. Washing and care products using this essence have good inhibitory effects on harmful bacteria, build a natural biological protective film on the skin surface, and have anti-inflammatory, soothing, repairing, anti-itch, and redness-reducing properties, leaving the skin moisturized and firm.

The method provides a skin barrier repairing composition derived from an extreme environment, combines Antarctic chlorella extract, ectoine and glycerol glucoside, the composition has a significant synergistic effect in repairing the skin barrier, and the cosmetic applying the composition has excellent repairing effect, fundamentally solves a series of problems of the skin, and has a good application prospect.

This invention provides an engineered bacterium for producing ectoine, its preparation method, and its application. The engineered bacterium is prepared using a method comprising the following steps: using *Corynebacterium glutamicum* as the starting strain, feedback inhibition by aspartate kinase LysC is relieved, the activity of the gene lysE encoding lysine efflux permease is reduced, and the ectoine synthesis gene cluster ectABC is expressed. Based on this, the activity of any one or at least two of the following—diaminobutyrate acetyltransferase EctA, pyruvate carboxylase Pyc, or aspartate kinase LysC—is enhanced, and / or the activity of any one or at least two of the following—maloquinone oxidoreductase Mqo or homoserine dehydrogenase Hom—is reduced to further increase ectoine yield.

The invention provides a method for producing Ectoine through salt impact-glutamine synergistic feeding, and relates to the field of fermentation engineering. The invention particularly relates to a metabolism regulation strategy of salt impact in cooperation with glutamine supplement. According to the method, after salt impact is applied in the logarithmic phase of halophilic bacteria, glutamine is accurately supplemented to activate a thallus stress synthesis pathway. Through the method, the yield of Ectoin can be remarkably increased, the fermentation period is shortened, and the metabolic activity of thalli is enhanced. The method is simple and convenient to operate and controllable in cost, and an innovative process solution is provided for efficient production of industrial fermentation of the halophilic bacteria.

This invention discloses a repair composition containing multiple ceramides and its preparation method, belonging to the field of cosmetic technology. The repair composition, by mass parts, comprises ceramide AP, ceramide EOP, ceramide NP, phytosphingosine, Centella asiatica extract, Portulaca oleracea extract, Hydrangea macrophylla extract, ectoine, and tripeptide-1. This invention utilizes multiple ceramides and phytosphingosine to biomimetically replenish the lipids of the stratum corneum, combined with the synergistic effects of various plant active ingredients and active peptides, to effectively achieve multiple effects such as skin barrier repair, soothing and anti-inflammatory properties, and anti-allergy. This invention also provides a barrier repair serum containing this repair composition and its preparation method. The serum has stable physicochemical properties and, verified by in vitro cell experiments and human efficacy tests, can rapidly repair damaged skin barriers, improve sensitive symptoms such as redness and stinging, and is gentle and non-irritating, suitable for daily skincare use by people with sensitive skin and damaged skin barriers.

The invention belongs to the technical field of biomedical materials, and particularly relates to an antioxidant for improving the stability of mussel mucin and application of the antioxidant. The antioxidant provided by the invention is a combination of an antioxidant A and an antioxidant B, or a combination of the antioxidant A, the antioxidant B and an antioxidant C. The antioxidant A is composed of Ectoine, mussel mucin type 6 (Mfp-6), glycerin and polyvinylpyrrolidone (PVP), the antioxidant B is an antioxidant containing reduced sulfur, and the antioxidant C is a hydrogen supply type antioxidant. The mussel mucin is protected by the components in the antioxidant through multiple mechanisms, so that the oxidation degree and the oxidation rate of the mussel mucin in the mussel mucin preparation are obviously slowed down.

The invention relates to the field of microbial fermentation engineering, and particularly discloses a stirring paddle combination method and system for optimizing fermentation of halophilic bacteria to produce Ectoine. Through CFD simulation and comparison of eight three-layer paddle combinations, it is found that a CD6-RT-RT combination with a semicircular tube paddle (CD6) arranged on the bottom layer and six flat blade paddles (RTs) arranged on the middle layer and the top layer can synergistically improve the gas-liquid mass transfer efficiency of a high-salt system, bubbles are broken through strong radial flow of the CD6 paddle on the top layer, and a gas-liquid interface is expanded in combination with high-shear axial flow of the RT paddles on the middle layer and the bottom layer; uniform distribution of the gas content (gradient is less than or equal to 5%) and kLegt are realized; compared with a traditional combination, the efficiency is improved by 25%-72%; in a 50L tank, the yield of Ectocaine reaches 32.2 g / L, which is obviously improved by 15.0% compared with that of a conventional RT-RT-RT combination. According to the method, the oxygen transfer bottleneck of high-salt fermentation is improved, and an efficient and low-consumption scheme is provided for industrial production of Ectoin.

This invention relates to the field of separation and purification technology, and in particular to a method for separating and purifying ectoine from fermentation broth. This invention provides an integrated purification process combining aqueous two-phase extraction technology with antisolvent-temperature coupled crystallization technology, which can solve the problems of poor selectivity in separating ectoine from impurities and difficulty in controlling the crystallization process, ultimately achieving a balance between high yield (≥95%) and high purity (≥99.5%).

The invention discloses an eye protection external preparation and a preparation method thereof, and belongs to the technical field of ophthalmic preparations. The preparation method comprises the following steps: mixing a main agent solution A containing Ectoine and trehalose with a matrigel solution B containing chitosan quaternary ammonium salt; and then carrying out high-pressure microjet homogenization treatment on the mixed solution at 100-150 MPa so as to construct a supramolecular network structure. Through a specific high-pressure homogenization process, the obtained preparation has an excellent shear thinning characteristic, high viscosity is kept during standing to realize biological adhesion, the viscosity is instantaneously reduced during blinking to ensure comfort, and the problem of blurred vision of traditional gel is solved. Meanwhile, due to the network structure of the preparation, the active ingredients can be instantly released, the hypertonic state of the eyes can be rapidly relieved, and the preparation is adhered to the ocular surface for a long time.

The present invention relates to the field of microbial technology, and specifically discloses a recombinant microorganism and its application in the fermentative production of ectoine. Compared with the starting strain, the recombinant microorganism of the present invention expresses the ectABC gene of Chromohalobacter salexigens, expresses the glucose facilitator gene of Zymomonas mobilis glf while reducing the ptsG gene expression, and also overexpresses glucokinase glk, preferably also overexpresses phosphoenolpyruvate carboxylase ppc, and more preferably replaces the original promoter of the isocitrate dehydrogenase gene icd with a growth-phase-dependent promoter. Without significantly affecting the growth performance of the bacterial cells, the recombinant microorganism of the present invention can significantly improve the yield and productivity of ectoine in fermentative production, thereby enhancing the efficiency of industrial production of ectoine and reducing the production cost.

The invention belongs to the technical field of organic chemistry, and discloses a preparation method of high-purity ectoine, which comprises the following steps: by taking L-glutamine as a raw material, carrying out amino protection under an alkaline condition, carrying out Hofmann degradation carbonyl removal reaction without separation, carrying out secondary Boc protection without separation, and carrying out double-Boc protection on L-2, 4, 6-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5- The method comprises the following steps: extracting L-2, 4-diaminobutyric acid into an organic phase by using an organic solvent, directly introducing a proper amount of hydrogen chloride gas into the combined organic phase, and simultaneously completing deprotection and separating out an L-2, 4-diaminobutyric acid dihydrochloride intermediate. The preparation process has the characteristics of being high in yield, simple and convenient in post-treatment, environment-friendly, high in purity and the like; the separated-out L-2, 4-diaminobutyric acid dihydrochloride solid does not need to be refined and is directly subjected to cyclization reaction with trimethyl orthoacetate to generate the target product Ectoine. The preparation method provided by the invention is low in production cost, efficient and environment-friendly.

The invention relates to a composition for post-art repair and application of the composition. The composition is prepared from dipotassium glycyrrhizinate, sodium hyaluronate, sodium polyglutamate, kava pepper leaf / root / stem extract, ectoine, chitosan, tranexamic acid, tremella sporocarp extract and betaine. The composition provided by the invention can achieve the synergistic effects of instantly relieving, quickly repairing a physical barrier, recovering balance of a microbial barrier and preserving moisture for a long time.

The present invention relates to the field of bioengineering, and particularly to an enzyme composition, a product and their application in the preparation of ectoine. The present invention provides an enzyme composition, comprising: a mutant of 2-hydroxy-4-aminobutyric acid oxidase, a mutant of keto transaminase, a mutant of hydrogen peroxide hydrolase, a mutant of ectoine synthase, a mutant of pyruvate-CoA oxidase, and Lactobacillus helveticus lactate dehydrogenase. The present invention for the first time uses the combined transformation of multiple mutant enzymes to rapidly and efficiently produce ectoine with inexpensive 2-hydroxy-4-aminobutyric acid as the starting material; because the enzymatic method has characteristics such as being easy to control, short transformation time, and few generated impurities, this method has unique advantages in the inexpensive large-scale production of ectoine.

The present invention relates to the technical field of nasal mucosa repair, specifically to a preparation method of functional microspheres wrapped with composite protein and its application in nasal mucosa repair. The present invention uses silk fibroin, sodium chloride, ectoine, sorbitol, resveratrol, borneol, curcumin, volatile oil of Magnolia officinalis, volatile oil of Xanthium sibiricum, volatile oil of Centipeda minima, bioactive glass powder, and mussel adhesive protein as raw materials. Utilizing the negatively charged property of the nasal mucosa, functional microspheres that can have a strong electrostatic adsorption effect with the nasal mucosa are prepared. A biofilm is formed on the surface of the nasal mucosa by the functional microspheres, which can prevent and reduce the contact between pathogenic microorganisms and other particulate allergic substances in the external environment and the nasal mucosa. At the same time, electrostatic adsorption can also prolong the action time of functional components on the nasal mucosa. The functional components play roles from different aspects successively, enhancing their action effects on the nasal mucosa. The functional microspheres obtained by the preparation method of the present invention can effectively treat various rhinitis, improve the symptoms of sneezing, rhinorrhea, nasal congestion, etc. in patients, and have a long-lasting effect, are not easy to relapse, have little irritation, and high safety.

The invention relates to the technical field of biochemical engineering, in particular to a process method for producing ectoine through efficient fermentation. According to the method, a culture medium formula of a base material culture medium and trace elements is developed by optimizing the culture medium, and the base material culture medium comprises glucose, ammonium sulfate, ammonium dihydrogen phosphate, citric acid, potassium sulfate, magnesium sulfate, ferrous sulfate, manganese sulfate, ampicillin and streptomycin; the microelements comprise vitamin B1, vitamin B6, NiCl2, CuSO4, CoCl2 and NaMoO4, and the culture medium disclosed by the invention can be used for remarkably improving the metabolic activity of thalli and has a positive effect on improving the yield and the conversion rate of ectoine; according to the method, the yield of ectoine is high through the ectoine synthesis strain, the fermentation dissolved oxygen and the rotating speed are controlled in a segmented mode in the fermentation process, the yield of the ectoine synthesis strain reaches 134.29 g / L and is successfully amplified to the industrial scale, the fermentation content of a 5m < 3 > tank reaches 90.13 g / L, and the method has great industrialization value.

The invention relates to an Ectoine-producing halophilic bacterium as well as a screening method and application thereof, and belongs to the technical field of microorganisms. According to the Ectoin-producing halophilic bacteria disclosed by the invention, the Ectoin-producing halophilic bacteria are Vreelandelassp., and the Ectoin-producing halophilic bacteria are Ectoin-producing halophilic bacteria. The 16S rRNA of the Ectoin-producing halophilic bacteria has at least 90% of nucleotide sequence consistency with that of SEQ ID NO. 1; the Ectoin halophilic bacteria are used for expressing 2-aminobutyric acid acetyltransferase, 2-aminobutyric acid transaminase and Ectoin synthetase. Through an efficient screening method and deep genome analysis, the novel halophilic strain capable of synthesizing the Ectoine is successfully screened out, and the gene cluster for synthesizing the Ectoine is excavated, so that the production efficiency and yield of the Ectoine can be improved, a microbial resource library is enriched, and the genetic diversity is increased.

The invention discloses an ectoine hydroxylase mutant EctD-1 and application, and belongs to the technical field of synthetic biology. The amino acid sequence of the mutant is as shown in SEQ ID NO.1, the coding gene is ectD-1, and the nucleotide sequence is as shown in SEQ ID NO.2. The application of the ectoine hydroxylase mutant EctD-1 in synthesis of 5-hydroxyectoine comprises the following steps: (1) constructing a recombinant vector containing the ectoine hydroxylase mutant EctD-1; (2) constructing a recombinant strain containing the ectoine hydroxylase mutant EctD-1; and (3) carrying out fermentation production on the 5-hydroxyectoine by utilizing a recombinant strain containing the ectoine hydroxylase mutant EctD-1. The ectoine hydroxylase mutant provided by the invention is high in activity, the efficiency of catalyzing conversion of ectoine into 5-hydroxyectoine is high, and the synthesis efficiency of the 5-hydroxyectoine is remarkably improved.

The present invention relates to the technical field of biochemical detection, and discloses a nucleic acid detection composition for non-small cell lung cancer carboplatin resistance screening, the nucleic acid detection composition comprises a specific amplification primer pair of a targeting NFAT2 gene, a TaqMan detection probe, a hot start TaqDNA polymerase and a reaction buffer solution, the reaction buffer solution comprises ectoine and tetramethylammonium chloride, according to the method, carboplatin is blocked by a compact hydrated shell layer formed on the surface of enzyme through ectoine, and the hydrogen bond energy difference between basic groups of nucleic acid large grooves is eliminated by using tetramethylammonium chloride, so that the microcosmic functional phase splitting structure is constructed. Therefore, efficient protection of polymerase activity and regulation and control of primer hybridization specificity are synchronously realized in a single reaction system, and sensitivity and accuracy of drug-resistant gene detection in a complex clinical sample are improved.

The invention discloses a high-yield biological preparation device of Ectoine and a preparation method of the high-yield biological preparation device, and relates to the technical field of Ectoine production. The device comprises a fermentation detection assembly, an automatic cover taking assembly is arranged on the fermentation detection assembly, a wiping assembly is arranged on the fermentation detection assembly, a sampling assembly is arranged above the fermentation detection assembly, and a transmission assembly is arranged on the fermentation detection assembly; the fermentation detection assembly includes a conduit. Through the use of the device, automatic sampling of a microorganism mixed solution for preparing Ectoine is realized, the operation amount of workers is reduced, the production efficiency is improved, and through the cooperative use of the automatic cover taking assembly and the wiping assembly, the first sealing cover is taken down, transferred and disinfected; therefore, the use convenience is improved, the sterility of the first sealing cover is ensured, safe fermentation of microorganisms is ensured, in addition, in cooperation with the use of the sampling assembly, automatic sampling and detection of a microorganism mixed solution are achieved, and the detection efficiency is improved.

To provide an external composition superior in stability of ectoine.SOLUTION: An external composition containing ectoine and at least one selected from the group consisting of a heparin analogue, glycyrrhizic acid and / or a salt thereof, ascorbyl glucoside, and allantoin.SELECTED DRAWING: None

This invention provides an engineered bacterium for producing ectoine, its preparation method, and its application. The engineered bacterium is prepared using the following steps: using Corynebacterium glutamicum as the starting strain, feedback inhibition of aspartate kinase LysC is relieved, the activity of the gene lysE encoding lysine efflux permease is reduced, and the ectoine synthesis gene cluster ectABC is expressed; based on this, the activity of any one or at least two combinations of aspartate semialdehyde dehydrogenase Asd, aspartate transaminase AspB, or 6-phosphogluconate dehydrogenase Gnd is enhanced to further increase the yield of ectoine.

The invention provides an engineering bacterium for producing Ectoine as well as a preparation method and application thereof, and the engineering bacterium is prepared by the preparation method comprising the following steps: taking corynebacterium glutamicum as a starting strain, relieving feedback inhibition of aspartate kinase LysC, reducing activity of gene lysE for coding lysine efflux permease, and obtaining the Ectoine-producing engineering bacterium. And expressing an Ectoin synthetic gene cluster ectABC. On the basis, the activity of any one or a combination of at least two of diaminobutyric acid acetyltransferase EctA, pyruvate carboxylase Pyc or aspartate kinase LysC is enhanced, and / or the activity of any one or a combination of two of malic acid quinone oxidoreductase Mqo or homoserine dehydrogenase Hom is reduced, so that the yield of Ectoine is further improved.

The invention discloses a composition for gene methylation detection, a kit, application and a detection method, the composition comprises: 50-100 mM Tris HCl, the pH value of which is 9.0; 0.5 to 2.5 mM of dNTPs (deoxyribonucleoside 100 to 300 mM of KCl; 7.5 to 15 mM of MgCl2; 2.5%-5% of DMSO (dimethylsulfoxide); 100 to 300 mM of tetramethylammonium chloride; 2.0 to 5.0 mM of Ectocaine; and 2.0 to 4.0 M of proline. By using the composition and the gene methylation detection method provided by the invention, the enzyme digestion reaction and the qPCR reaction can be simultaneously carried out in one tube, so that not only is the efficiency of the enzyme digestion reaction and the specificity of gene methylation detection ensured, but also nucleic acid damage and loss of a large amount of nucleic acid caused by a sulfite conversion process are avoided; the sensitivity of gene methylation detection is obviously improved; meanwhile, the detection method has the characteristics of simplicity in operation and short time consumption.

The present invention discloses a method for regulating the synthesis of ectoine in Escherichia coli, which relates to the technical field of biomedicine. The method for regulating the synthesis of ectoine in Escherichia coli disclosed by the present invention constructs a tisB toxin protein expression vector pET28a(+)-p2-22-tisB with a gene fragment of the tisB toxin protein, and transfers it into an Escherichia coli engineering bacterium capable of synthesizing ectoine, so as to obtain an Escherichia coli engineering bacterium E. coli Ect pET28a(+)-p2-22-tisB that spontaneously inhibits the synthesis of ectoine, and at the same time can ensure normal synthesis of ectoine in the early stage. The nucleotide sequence of the gene fragment of the tisB toxin protein is as shown in SEQ ID NO.1. In the first three rounds of fermentation, the yield of ectoine is similar to that of the control engineering bacterium; after the seventh round, the yield of ectoine drops sharply to 2.575 g / L, and in the twentieth round of fermentation, the yield of ectoine is zero.

The invention discloses an L-2, 4-diaminobutyric acid transaminase mutant 2423-EctB-H and application of the mutant 2423-EctB-H, and belongs to the technical field of biological engineering. The amino acid sequence of the mutant is as shown in SEQ ID NO.1, the coding gene of the mutant is 2423-ectB-H, and the nucleotide sequence of the mutant is as shown in SEQ ID NO.2. Compared with the original L-2, 4-diaminobutyric acid transaminase, the L-2, 4-diaminobutyric acid transaminase mutant 2423-ectB-H provided by the invention has the advantages that the activity is enhanced, and the efficiency of catalytically generating L-2, 4-diaminobutyric acid by taking L-aspartic acid-beta-semialdehyde as a substrate is obviously improved; the strain is inserted at the position of the escherichia coli thrA gene and is used for fermenting ectoine, and the fermentation content of ectoine can be remarkably increased.

AMENDMENTS TO THE ABSTRACTThe invention relates to a composition containing, as active ingredient, ectoine, hydroxyectoine and / or salts, esters or amides of these compounds for the treatment and / or prophylaxis of diseases of the eye that are associated with a disturbance of the tear film. The disease can be in particular keratoconjunctivitis sicca. It has been found that, surprisingly, these compounds are capable of preventing a rupture in the outer lipid layer of the tear film, avoiding an undesirably rapid evaporation of the lacrimal fluid.