20 results about "Ectoine" patented technology

This invention discloses a coconut oil antibacterial and deodorizing essence, its preparation method, and its application. The essence consists of: 12-50% coconut oil, 4-10% butylene glycol, 0.1-2% sodium hyaluronate, 1-5% panthenol, 2-5% lactic acid, 2-5% potassium lactate, 1.5-6% emulsifier, 0.1-0.6% 4-tert-butylcyclohexanol, 0.1-6% ectoine, 0.1-6% ergothioneine, 1-2% non-irritating preservative composition, and the remainder being pure water. The components work synergistically. Disposable sanitary products using this essence can inhibit bacteria and deodorize, soften the skin, relieve itching, improve redness, and soothe sensitivity. Washing and care products using this essence have good inhibitory effects on harmful bacteria, build a natural biological protective film on the skin surface, and have anti-inflammatory, soothing, repairing, anti-itch, and redness-reducing properties, leaving the skin moisturized and firm.

This invention provides an engineered bacterium for producing ectoine, its preparation method, and its application. The engineered bacterium is prepared using a method comprising the following steps: using *Corynebacterium glutamicum* as the starting strain, feedback inhibition by aspartate kinase LysC is relieved, the activity of the gene lysE encoding lysine efflux permease is reduced, and the ectoine synthesis gene cluster ectABC is expressed. Based on this, the activity of any one or at least two of the following—diaminobutyrate acetyltransferase EctA, pyruvate carboxylase Pyc, or aspartate kinase LysC—is enhanced, and / or the activity of any one or at least two of the following—maloquinone oxidoreductase Mqo or homoserine dehydrogenase Hom—is reduced to further increase ectoine yield.

This invention discloses a repair composition containing multiple ceramides and its preparation method, belonging to the field of cosmetic technology. The repair composition, by mass parts, comprises ceramide AP, ceramide EOP, ceramide NP, phytosphingosine, Centella asiatica extract, Portulaca oleracea extract, Hydrangea macrophylla extract, ectoine, and tripeptide-1. This invention utilizes multiple ceramides and phytosphingosine to biomimetically replenish the lipids of the stratum corneum, combined with the synergistic effects of various plant active ingredients and active peptides, to effectively achieve multiple effects such as skin barrier repair, soothing and anti-inflammatory properties, and anti-allergy. This invention also provides a barrier repair serum containing this repair composition and its preparation method. The serum has stable physicochemical properties and, verified by in vitro cell experiments and human efficacy tests, can rapidly repair damaged skin barriers, improve sensitive symptoms such as redness and stinging, and is gentle and non-irritating, suitable for daily skincare use by people with sensitive skin and damaged skin barriers.

The invention belongs to the technical field of biomedical materials, and particularly relates to an antioxidant for improving the stability of mussel mucin and application of the antioxidant. The antioxidant provided by the invention is a combination of an antioxidant A and an antioxidant B, or a combination of the antioxidant A, the antioxidant B and an antioxidant C. The antioxidant A is composed of Ectoine, mussel mucin type 6 (Mfp-6), glycerin and polyvinylpyrrolidone (PVP), the antioxidant B is an antioxidant containing reduced sulfur, and the antioxidant C is a hydrogen supply type antioxidant. The mussel mucin is protected by the components in the antioxidant through multiple mechanisms, so that the oxidation degree and the oxidation rate of the mussel mucin in the mussel mucin preparation are obviously slowed down.

This invention relates to the field of separation and purification technology, and in particular to a method for separating and purifying ectoine from fermentation broth. This invention provides an integrated purification process combining aqueous two-phase extraction technology with antisolvent-temperature coupled crystallization technology, which can solve the problems of poor selectivity in separating ectoine from impurities and difficulty in controlling the crystallization process, ultimately achieving a balance between high yield (≥95%) and high purity (≥99.5%).

The invention belongs to the technical field of organic chemistry, and discloses a preparation method of high-purity ectoine, which comprises the following steps: by taking L-glutamine as a raw material, carrying out amino protection under an alkaline condition, carrying out Hofmann degradation carbonyl removal reaction without separation, carrying out secondary Boc protection without separation, and carrying out double-Boc protection on L-2, 4, 6-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5-trimethyl-1, 3, 5- The method comprises the following steps: extracting L-2, 4-diaminobutyric acid into an organic phase by using an organic solvent, directly introducing a proper amount of hydrogen chloride gas into the combined organic phase, and simultaneously completing deprotection and separating out an L-2, 4-diaminobutyric acid dihydrochloride intermediate. The preparation process has the characteristics of being high in yield, simple and convenient in post-treatment, environment-friendly, high in purity and the like; the separated-out L-2, 4-diaminobutyric acid dihydrochloride solid does not need to be refined and is directly subjected to cyclization reaction with trimethyl orthoacetate to generate the target product Ectoine. The preparation method provided by the invention is low in production cost, efficient and environment-friendly.

The invention relates to a composition for post-art repair and application of the composition. The composition is prepared from dipotassium glycyrrhizinate, sodium hyaluronate, sodium polyglutamate, kava pepper leaf / root / stem extract, ectoine, chitosan, tranexamic acid, tremella sporocarp extract and betaine. The composition provided by the invention can achieve the synergistic effects of instantly relieving, quickly repairing a physical barrier, recovering balance of a microbial barrier and preserving moisture for a long time.

The invention relates to an Ectoine-producing halophilic bacterium as well as a screening method and application thereof, and belongs to the technical field of microorganisms. According to the Ectoin-producing halophilic bacteria disclosed by the invention, the Ectoin-producing halophilic bacteria are Vreelandelassp., and the Ectoin-producing halophilic bacteria are Ectoin-producing halophilic bacteria. The 16S rRNA of the Ectoin-producing halophilic bacteria has at least 90% of nucleotide sequence consistency with that of SEQ ID NO. 1; the Ectoin halophilic bacteria are used for expressing 2-aminobutyric acid acetyltransferase, 2-aminobutyric acid transaminase and Ectoin synthetase. Through an efficient screening method and deep genome analysis, the novel halophilic strain capable of synthesizing the Ectoine is successfully screened out, and the gene cluster for synthesizing the Ectoine is excavated, so that the production efficiency and yield of the Ectoine can be improved, a microbial resource library is enriched, and the genetic diversity is increased.

The present invention relates to the technical field of biochemical detection, and discloses a nucleic acid detection composition for non-small cell lung cancer carboplatin resistance screening, the nucleic acid detection composition comprises a specific amplification primer pair of a targeting NFAT2 gene, a TaqMan detection probe, a hot start TaqDNA polymerase and a reaction buffer solution, the reaction buffer solution comprises ectoine and tetramethylammonium chloride, according to the method, carboplatin is blocked by a compact hydrated shell layer formed on the surface of enzyme through ectoine, and the hydrogen bond energy difference between basic groups of nucleic acid large grooves is eliminated by using tetramethylammonium chloride, so that the microcosmic functional phase splitting structure is constructed. Therefore, efficient protection of polymerase activity and regulation and control of primer hybridization specificity are synchronously realized in a single reaction system, and sensitivity and accuracy of drug-resistant gene detection in a complex clinical sample are improved.

The invention discloses a high-yield biological preparation device of Ectoine and a preparation method of the high-yield biological preparation device, and relates to the technical field of Ectoine production. The device comprises a fermentation detection assembly, an automatic cover taking assembly is arranged on the fermentation detection assembly, a wiping assembly is arranged on the fermentation detection assembly, a sampling assembly is arranged above the fermentation detection assembly, and a transmission assembly is arranged on the fermentation detection assembly; the fermentation detection assembly includes a conduit. Through the use of the device, automatic sampling of a microorganism mixed solution for preparing Ectoine is realized, the operation amount of workers is reduced, the production efficiency is improved, and through the cooperative use of the automatic cover taking assembly and the wiping assembly, the first sealing cover is taken down, transferred and disinfected; therefore, the use convenience is improved, the sterility of the first sealing cover is ensured, safe fermentation of microorganisms is ensured, in addition, in cooperation with the use of the sampling assembly, automatic sampling and detection of a microorganism mixed solution are achieved, and the detection efficiency is improved.

This invention provides an engineered bacterium for producing ectoine, its preparation method, and its application. The engineered bacterium is prepared using the following steps: using Corynebacterium glutamicum as the starting strain, feedback inhibition of aspartate kinase LysC is relieved, the activity of the gene lysE encoding lysine efflux permease is reduced, and the ectoine synthesis gene cluster ectABC is expressed; based on this, the activity of any one or at least two combinations of aspartate semialdehyde dehydrogenase Asd, aspartate transaminase AspB, or 6-phosphogluconate dehydrogenase Gnd is enhanced to further increase the yield of ectoine.

The invention provides an engineering bacterium for producing Ectoine as well as a preparation method and application thereof, and the engineering bacterium is prepared by the preparation method comprising the following steps: taking corynebacterium glutamicum as a starting strain, relieving feedback inhibition of aspartate kinase LysC, reducing activity of gene lysE for coding lysine efflux permease, and obtaining the Ectoine-producing engineering bacterium. And expressing an Ectoin synthetic gene cluster ectABC. On the basis, the activity of any one or a combination of at least two of diaminobutyric acid acetyltransferase EctA, pyruvate carboxylase Pyc or aspartate kinase LysC is enhanced, and / or the activity of any one or a combination of two of malic acid quinone oxidoreductase Mqo or homoserine dehydrogenase Hom is reduced, so that the yield of Ectoine is further improved.

The invention discloses an L-2, 4-diaminobutyric acid transaminase mutant 2423-EctB-H and application of the mutant 2423-EctB-H, and belongs to the technical field of biological engineering. The amino acid sequence of the mutant is as shown in SEQ ID NO.1, the coding gene of the mutant is 2423-ectB-H, and the nucleotide sequence of the mutant is as shown in SEQ ID NO.2. Compared with the original L-2, 4-diaminobutyric acid transaminase, the L-2, 4-diaminobutyric acid transaminase mutant 2423-ectB-H provided by the invention has the advantages that the activity is enhanced, and the efficiency of catalytically generating L-2, 4-diaminobutyric acid by taking L-aspartic acid-beta-semialdehyde as a substrate is obviously improved; the strain is inserted at the position of the escherichia coli thrA gene and is used for fermenting ectoine, and the fermentation content of ectoine can be remarkably increased.

The invention discloses application of Ectoin in preparation of a medicine for prolonging the service life, and belongs to the technical field of biology. Life experiments prove that the life of caenorhabditis elegans can be obviously prolonged by 25.8% by 5mM of Ectoine; 10 mM of Ectoine can obviously prolong the service life of caenorhabditis elegans, and the service life is prolonged by 43.4%; 15mM Ectoine can obviously prolong the service life of caenorhabditis elegans, and the service life is prolonged by 21.1%. The invention proves that Ectoin has an excellent effect of prolonging the service life, so that related products can be effectively developed on the basis of the medicine, and the Ectoin has wide application prospects and popularization values.

This invention provides a method for synthesizing ectoine using moderately halophilic bacteria through fermentation. It innovatively introduces a concentration reaction system, integrating the "cultivation + concentration" of halophilic bacteria into one process. This eliminates the traditional steps of batch feeding and concentration, greatly increasing the yield and efficiency of ectoine synthesis. It enables inexpensive wild-type halophilic bacteria to achieve a level comparable to that of expensive genetically engineered halophilic bacteria, thus significantly reducing the cost of ectoine synthesis.

The application discloses a construction method of a trichoderma reesei engineering bacterium for producing ectoine and application thereof, and belongs to the technical field of bioengineering. The application provides a construction method of a trichoderma reesei engineering bacterium for producing ectoine, which comprises the following steps: taking trichoderma reesei as a starting strain, and introducing an ectoine synthesis gene cluster into the trichoderma reesei to construct the trichoderma reesei engineering bacterium; and the nucleotide sequence of the ectoine synthesis gene cluster is shown as SEQ ID NO. 1. The application enables the trichoderma reesei which originally does not have the ectoine synthesis capacity to produce ectoine; and by increasing the number of ectoine gene clusters and optimizing the culture medium, the yield of ectoine is significantly improved, wherein, glucose is used as a carbon source, protein peptone and urea are used as long-acting nitrogen sources, and the addition amount of the nitrogen source is optimized, and the yield of ectoine in flask fermentation can reach 1494.5 mg / L, thereby providing technical support for industrialized production of ectoine.

The invention provides a composition containing trehalose-6-phosphoric acid and application of the composition in prevention and control of high-temperature heat damage to crops, based on 100 parts by weight of the total weight of the composition, the composition comprises 0.25 parts by weight or more of trehalose-6-phosphoric acid, 0.5 parts by weight or more of Ectoine and 2.6 parts by weight or more of chelated calcium, the weight of the chelated calcium is based on the weight of calcium contained in the chelated calcium. The composition can reduce yellowing and withered leaves of crops and reduce the blighted grain rate, has environmental friendliness and application convenience, and has wide application prospects in prevention and control of high-temperature heat damage of the crops.

The invention provides an engineering bacterium for producing Ectoine as well as a preparation method and application thereof, and the engineering bacterium is prepared by the preparation method comprising the following steps: taking corynebacterium glutamicum as a starting strain, relieving feedback inhibition of aspartate kinase LysC, reducing activity of gene lysE for coding lysine efflux permease, and obtaining the Ectoine-producing engineering bacterium. And expressing an Ectoin synthetic gene cluster ectABC. On the basis, the activity of any one or a combination of at least two of diaminobutyric acid acetyltransferase EctA, pyruvate carboxylase Pyc or aspartate kinase LysC is enhanced, and / or the activity of any one or a combination of two of malic acid quinone oxidoreductase Mqo or homoserine dehydrogenase Hom is reduced, so that the yield of Ectoine is further improved.

The invention relates to a saline-alkali resistant fertilizer containing Ectoine and Bacillus velezensis and a preparation method thereof, and belongs to the technical field of saline-alkali resistant fertilizers. The salt and alkali resistant fertilizer disclosed by the invention mainly comprises the following components in parts by weight: 0.1-0.5 part of ectoin, 5-10 parts of bacillus velezensis, 0.5-5 parts of a protective agent, 20-150 parts of nitrogen phosphorus and potassium nutrients, 10-30 parts of a modifier and 0.3-1.3 parts of an auxiliary material. The saline-alkali resistant fertilizer provided by the invention has obvious saline-alkali resistant effect and plant growth promoting effect, can effectively improve the soil environment of saline-alkali soil, and provides favorable conditions for crop growth.

The present application provides an oil control composition and use thereof. It is found in the present application that ectoine has a significant inhibitory effect on sebum production caused by linoleic acid. Therefore, ectoine can be used as an oil control active ingredient.