52 results about "Halomonas" patented technology

The invention discloses a halomonas strain and application thereof. The halomonas strain is a halomonas sp. TD01 with a preservation number of CGMCC NO.4353. Experiments indicate that the halomonas sp. TD01 can effectively accumulate polyhydroxyalkanoates (PHA) in a mineral medium (MM) and provides a better guarantee for the biosynthesis of the PHA and PHBV (poly(hydroxybutyrate-hydroxyvalerate)). The halomonas strain has the advantages of simple nutrient requirement, no need of sterilization in a fermenting process, capability of continuous implantation and simpleness and easiness in control. Methods for preparing the PHA by using the halomonas sp. TD01 all lower the production cost from varying degrees and increase the yield of the PHA; and the obtained PHA has the molecular weight of above 500kDa and has an industrial application value.

The invention relates to a genetically engineered bacterium producing tetrahydropyrimidine and a structuring method and application thereof. The genetically engineered bacterium is Escherichia coli with a specific genotype, comprises Halomonas-elogata-derived ectABC gene, has three gene deficiency variants including lysA, thrA and iclR and has corynebacterium glutamicum lysC gene controlled by a lac promoter and ppc gene controlled by a trc promoter. The defect that chemical synthesis methods have harsh reaction conditions and high energy consumption can be overcome by using the genetically engineered bacterium and utilizing glucose fermentation to produce tetrahydropyrimidine. The defect that Halomonas-elogata fermentation or enzyme-catalyzed methods are complex in process and high in production cost can be overcome. After fermentation for 20-28h, yield of tetrahydropyrimidine reaches 12-18g/L, and the genetically engineered bacterium has high industrial application value.

The invention relates to a genetically engineered bacterium for high-yield production of hydroxytetrahydropyrimidine as well as a construction method and application of the genetically engineered bacterium. The strain integrates RNA (Ribonucleic Acid) polymerase which is controlled by a xylose promoter and is derived from T7 bacteriophage; a thrA gene for encoding homoserine dehydrogenase I is knocked out; a lysC gene derived from corynebacterium glutamicum, an ectABC gene cluster derived from halomonas elongate and an ectD gene for encoding tetrahydropyrimidine hydroxylase are integrated andare promoted by a strong promoter PT7; a hydroxytetrahydropyrimidine synthesis way is reconstructed; the ectD gene is integrated at a sucCD site of succinyl coenzyme A synthetase and is started by thestrong promoter PT7; the genetically engineered bacterium has the highest level of producing the hydroxytetrahydropyrimidine by a fermentation method which is reported at present.

The invention provides a saline-alkaline tolerant compound active microbial flora, which is used to process waste drilling well slurry and oil-containing slurry with a high saline-alkaline content. The provided saline-alkaline tolerant active microbial flora contains shewanella mac donell, pseudomonas, and halomonas, wherein the preferable number ratio of shewanella mac donell to pseudomonas to halomonas is 4:3:1. The provided flora has a strong endurance capacity on pH and salt content, and can well adapt to the various environments of waste slurry in drilling well and soil polluted by petroleum. The additives and harmful substances in the waste slurry do not have a prominent inhibition effect on the active microbial flora. The growth speed of the flora is quick, the activity of the flora is stable, and the adaptability of the flora is strong. The production cost is low. The flora can be applied to the biodegradation of waste slurry in drilling wells, repairmen of environment destroyed by petroleum, and chemical engineering industry. The provided flora can increase the biodegradation efficiency on slurry in drilling wells and further improve the related processing technology.

The invention provides a bacteria halomonas huanghegensis BJGMM-B45, which has excellent salt tolerance. The preservation number of the halomonas huanghegensis BJGMM-B45 is CGMCC (China General Microbiological Culture Collection Center) NO.7022. The bacteria not only can be directly used as functional bacteria for improvement of saline-alkali soil, but also can be used as a gene donor of a novel transgenic organism; the organism can obtain excellent stress resistance if an excellent salt tolerance gene is transferred into the organism; in addition, the bacteria also can be used as a novel gene engineering bacteria, and can obtain more excellent properties by accepting other foreign excellent genes; furthermore, more mutant strains with excellent properties can be obtained through improvement of properties by traditional mutation breeding manner.

The invention discloses a high-salinity nitrogen-containing wastewater stress resistance auxiliary nitrogen removal method, and belongs to the technical field of microorganism nitrogen removal. By constructing a tetrahydro-2-pyrimidinone secreting type halomonas meridian and traditional denitrifier strain combined stress resistance auxiliary synchronous heterotrophic nitrification aerobic denitrification nitrogen removal mode, the high-salinity nitrogen-containing wastewater nitrogen removal technology is provided. The problem that according to existing high-salinity nitrogen-containing wastewater nitrogen removal, the nitrogen removal efficiency is low due to inhibition of high salinity on denitrifier strain growth and metabolism is solved, and the application prospect on the aspects of high-salinity nitrogen-containing wastewater treatment and seawater aquatic water purification is achieved.

Disclosed are a method for producing polyhydroxyalkanoates (PHAs) using a halobacterium belonging to the genus Halomonas, wherein the halobacterium can grow in a medium consisting of an inorganic salt and a single organic carbon source and having a pH of 8.8 to 11, and produce PHAs in an amount of 20 wt. % or more based on the dry cell weight, and the halobacterium is cultured in an alkaline medium containing an inorganic salt and one or more organic carbon sources to produce PHAs in an amount of 20 wt. % or more based on the dry cell weight; and the halobacterium belonging to the genus Halomonas, which can grow in a medium consisting an inorganic salt and a single organic carbon source and having a pH of 8.8 to 11, and produce PHAs in an amount of 20 wt. % or more based on the dry cell weight.

The invention discloses a strain for producing alginate lyase and a use thereof. The strain is preserved in the China general microbiological culture collection center on October 9, 2013, has an accession number of CGMCC No.8312 and is named as Halomonas elongata SH-19. A 16S rDNA polynucleotide sequence of the strain is shown in the formula of SEQ ID No.1. The strain SH-19 can efficiently express alginate lyase and can be used for high-efficiency production of alginate lyase. A strain SH-19 fermentation medium comprises simple ingredients commonly used in a laboratory, has substantial fermentation effects, greatly reduces an alginate lyase production cost and can be used for large-scale production.

The invention discloses a biochemical coupling-effect blocking remover and a preparation method thereof, and relates to the technical field of preparation of blocking removers. The blocking remover comprises a chemical composite blocking remover and a microbial oil displacement blocking remover; the chemical composite blocking remover is composed of the following raw material components: sodium polyepoxysuccinate, sodium ethylene diamine tetracetate, ammonium hydroxysuccinate, diisobutyl methanol, sodium allysulfonate, sodium lignin sulfonate and sodium succinic acid ester sulfonate. The microbial oil displacement blocking remover is composed of the following raw material components: halomonas, bacillus subtilis, bacillus chlamydosporium and pseudomonas. The blocking remover disclosed by the invention not only has an oil displacement function, but also has a blocking removing function of dissolving organic and inorganic blocking substances of stratum and recovering the permeability ofan oil layer; after the blocking remover enters the stratum, no by-product is generated, various material scales can be rapidly dissolved, and blocking removing products and working fluid do not needto flow back; the corrosion rate is less than 0.9 g/m<2>*h, the highest scale dissolving rate can reach 95%, and the highest rock core permeability recovery rate can reach 93.79%.

Ferment extract and exopolysaccharide of a bacterial strain for its use in treatment and/or care of the skin, as well as its cosmetic and/or dermopharmaceutical compositions. In particular, its use for skin aging, skin firmness and hydration.

The present invention discloses a halomonas aydingkolgenesis strain and an application thereof. The strain is halomonas aydingkolgenesis M1 and has a deposit number of CGMCC NO.19880 in the China General Microbiological Culture Collection Center. Studies show that the halomonas aydingkolgenesis M1can be cultured without sterilization under fermentation conditions in an opening way and can efficiently accumulate polyhydroxyalkanoate (PHA) and/or a small molecule compound of tetrahydropyrimidine, etc. A method for continuous culture of the halomonas aydingkolgenesis M1 is established and a method for co-producing the PHA and tetrahydropyrimidine is successfully established. The method by using the halomonas aydingkolgenesis M1 to produce the PHA and/or tetrahydropyrimidine can realize high yield, is low in production cost, and has broad application prospects.

The invention relates to a method for performing raw soil greening on coast saline land through a biological modifier containing fly ash. The biological modifier comprises the following components in weight part: 8-12 parts of ceramsite, 11-15 parts of vermiculite, 1-5 part of fly ash, 0.5-2 parts of iron chloride, 0.5-2 parts of calcium dihydrogen phosphate, 0.2-1 parts of formic acid, 1-4 parts of amino acid salt, 2-6 parts of fermented sheep manure, 2-7 parts of capsicum straw powder, 0.3-1 parts of compound sodium nitrophenolate, 0.3-1 parts of amino-oxyacetic acid, 0.3-1 parts of soybean germ extract, 0.5-2 parts of potassium ferrocyanide, 0.2-0.5 parts of cellulose cellulose grafted acrylic acid salt, 0.1-0.5 parts of halomonas, 0.1-0.5 parts of Bacillus subtilis, 0.1-0.5 parts of azotobacter vinelandii, 0.1-0.5 parts of Aspergillus oryzae and 0.1-0.5 parts of Trichoderma longibrachiatum.

The invention provides a biological seaweed fertilizer. The biological seaweed fertilizer is obtained by culturing a seaweed water extracting solution and a bacterial solution, wherein the bacterial liquid comprises halomonas, shewanella, Marinobacter, pseudomonas and nitrate reducing bacteria. Compared with the prior art, the microbial preparation is added into the seaweed water extracting solution for culture, plants can be promoted to absorb nutrient substances in the seaweed water extracting solution, then the quality of fruits and vegetables is improved, the content of soluble solids andVC is increased, fruit coloring is promoted, and the salt tolerance of the plants is improved; the preservation time of the biological seaweed fertilizer can be prolonged, and tropical agricultural areas are convenient to use.

The invention relates to the technical field of marine microorganisms. Since milk is generally conveyed and preserved at a low temperature, the capability of decomposing lactose at the low temperature is one of conditions which are necessary for industrially applying the beta-galactosidase. A marine halomonas strain P6006-1 provided by the invention is separated from sea water of an East China Sea region through culturing and has been preserved at the China Center for Type Culture Collection (CCTCC for short) in Wuhan city on January 7th, 2011, with a collection number of CCTCC No. M2011001. The invention also provides a method for producing the low-temperature beta-galactosidase by using the marine halomonas. The low-temperature beta-galactosidase produced by using the marine halomonas can be used for decomposing the lactose in cow milk; and the most proper reaction temperature is 30 DEG C.

The invention provides a process for producing 3-hydroxybutyric acid or a salt thereof, the process comprising culturing one or more halophilic bacteria belonging to the genus Halomonas under specific conditions.

The invention discloses a novel 5-enol pyruvyl shikimate3-phosphate (EPSP), the polynucleotide for encoding the EPSP and the method for producing the EPSP through recombination technology. The invention also discloses the use of the polynucleotide for encoding the EPSP.

The invention relates to secretions of marine halomonas, an extraction method of the secretions and the application of the secretions. The extraction method comprises the following steps: preparing a culture medium according to a formula of 5 g of yeast powder, 10 g of tryptone and 1L of seawater, and sterilizing the culture medium for 15-20 minutes at the temperature of 121 DEG C; inoculating the marine halomonas into the culture medium under aseptic conditions, culturing the culture medium inoculated with the marine halomonas for 10-15 days at the constant temperature of 25-30 DEG C on a backward rotating shaking table at the speed of 120-150 rpm, performing filtration with a film of 0.22-0.45 micrometers, and performing sterilization so as to obtain bacterial exudate supernatant; vacuum freezing and drying a filtrate, performing extraction through a pure acetic acid, and performing filtration and desalination through filter paper; volatilizing the acetic acid of the obtained filtrate so as to obtain coarse substances of the secretions of the marine halomonas. The secretions disclosed by the invention can be used for governing red tides, and have the effect of restraining dinoflagellates like Alexandrium tamarenses, prorocentrum donghaiense and Karenia mikimotoi of the red tides. According to the invention, the culture conditions are simple, products do not need to be refined, only the filtration is needed for removing thalli, and the secretions have a good effect of restraining the dinoflagellates of the red tides.

The invention discloses a Clostridium strain for producing acid. The strain is preserved in China Center for Type Culture Collection (CCTCC) in December 27, 2012, and the stain has a preserve number of CCTCC NO: M2012555 in Clostridium sp. YC3. The invention further discloses an application of a mixture of the strain and Halomonas to paper-making wastewater treatment. The stain disclosed by the invention not only has a capability of directly processing paper-making wastewater, but also can reduce the pH of the wastewater, thereby providing an agreeable environment for growth and colonization of methanogens in paper-making black liquid. According to the method which is a primary processing method, favorable conditions are provided for subsequent processes of the paper-making wastewater, the recovery efficiency is enhanced, the cost is lowered, and the secondary pollution is reduced; and the method has a wide application prospect in the paper-making wastewater treatment.

The invention discloses a heat-resistant moderately halophilic bacterium halomonas sp. LCB169 of which the preservation number is KCTC 52618. The strain is determined as a new species of halomonas bya polyphasic classification method. The halomonas bacterium disclosed by the invention has enzyme activities of oxidase, catalase, esterase, lipoesterase, leucine aminopeptidase, valine aminopeptidase, alkaline phosphatase and the like, and can be widely applied in related production fields of various bioengineering enzyme preparations. Moreover, the strain can grow normally at an NaCl concentration up to 17% and high temperature of 52 DEG C, indicating that the strain has high resistance to the environment. At the same time, the strain can secrete extracellular polysaccharides and accumulatepolyhydroxybutyrate abundantly under high NaCl concentration of up to 10%, so the strain can be further developed and applied to biomedicine and environmentally-friendly bioplastics related industriessuitable for extreme high-salt environments.

The present invention provides one kind of special basophile N19-2 and the microbe fermentation production process of alkaline mycose lyase by using sodium alginate and peptone as material in the presence of the special basophile N19-2 strain. The method is simple, the enzyme activity of the fermented liquid reaches 100 u/ml, and the post-extraction rate is over 80 %. The enzyme has optimal reaction pH value of 9.5, optimal reaction temperature of 55 deg.c and important application value in the production of oligomycose.

The invention discloses a special bio-organic fertilizer for rice planted saline-alkali soil. The special bio-organic fertilizer is prepared from halomonas, bacillus subtilis, paenibacillus polymyxa, bacillus thuringiensis, lactobacillus plantarum, Rubrivivax gelatinosus and an organic fertilizer. In actual preparation, microorganisms are propagated to obtain a microbial agent, then decomposed cow dung and auxiliary materials are uniformly mixed, granulation and drying are performed, cooling treatment is performed, a mixed solution of the microbial agent and a coating agent is added through a coating barrel for coating treatment, packaging is performed, and the finished product can be used after being packaged and stacked in a warehouse for a period of time. The bio-organic fertilizer has a high organic matter content and a high viable count, and can improve the soil structure of saline-alkali soil, reduce soil salinity and a pH value, enhance crop stress resistance, promote rice growth, increase yield and achieve the effects of soil improvement, fertility improvement, stress resistance and growth promotion when used in cooperation with conventional irrigation and fertilization measures.

The invention provides a quantitative detection method for halomonas strains by adopting an indirect enzyme-linked immunosordent assay. The quantitative detection method comprises the following steps: (1) preparing an O antigen of halomonas; (2) preparing a polyclonal antibody of the halomonas, namely with the O antigen of the halomonas as an immunizing antigen, immunizing a New Zealand rabbit, and preparing the polyclonal antibody of the halomonas by adopting an existing polyclonal antibody preparation and purification technology; and (3) carrying out an indirect ELISA operation method, namely coating the antigen with an elisa plate, and staying overnight at 4 DEG C, washing the plate, adding confining liquid, washing the plate, and adding the antibody, washing the plate, and adding a second antibody, washing the plate, and carrying out chromogenic reaction, and measuring the light absorption value, namely OD450 at a wavelength of 450nm by using a microplate reader. The quantitative detection method for the halomonas strains by adopting the indirect enzyme-linked immunosordent assay is simple and convenient in operation flow and is easy to operate, the detection sensitivity can reach 105cfu/mL, and antiserum generates no cross reaction with other bacteria, so that the quantitative detection method is a fast and efficient detection method with high sensitivity and strong specificity, promotes the application of probiotics on aquatic products and has the certain practical application significance.