1103 results about "Bacillus thuringiensis" patented technology

Disclosed are nucleic acid segments comprising synthetically-modified genes encoding modified Coleopteran-toxic B. thuringiensis Cry 3Bb* delta-endotoxins. Also disclosed are methods of using these genes for preparing a Coleopteran-resistant transgenic plant and reducing insect in restations, and plants thereby produced.

Disclosed is a novel delta -endotoxin, designated CryET29, that exhibits insecticidal activity against siphonapteran insects, including larvae of the cat flea (Ctenocephalides felis), as well as against colcopteran insects, including the southern corn rootworm (Diabrotica undecimpunctata), western corn rootworm (D. virgifera), Colorado potato beetle (Leptinotarsa decemlineata), Japanese beetle (Popillia japonica), and red flour beetle (Tribolium castaneur). Also disclosed are nucleic acid segments encoding CryET29, recombinant vectors, host cells, and transgenic plants comprising a cryET29 DNA segment. Methods for making and using the disclosed protein and nucleic acid segments are disclosed as well as assays and diagnostic kits for detecting cryET29 and CryET29 sequences in vivo and in vitro.

Disclosed are nucleic acid segments comprising synthetically-modified genes encoding Coleopteran-toxic B. thuringiensis delta -endotoxins. Also disclosed are methods of using these genes for the recombinant expression of polypeptides, the preparation of vectors containing the genes, and methods for transforming suitable host cells.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding δ-endotoxins having pesticidal activity against pests of the order Coleoptera. The invention further provides mutagenized nucleic acids that have been modified to encode endotoxins having improved pesticidal activity and / or altered pest specificity. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, expression cassettes comprising such nucleic acids, and transformed plants and seeds comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.

A method for expressing insecticidal protein structural genes in plant genomes is provided. In the preferred embodiments this invention comprises placing a structural gene for the Bacillus thuringiensis crystal protein under control of a plant or a T-DNA promoter and ahead of a polyadenylation site followed by insertion of said promoter / structural gene combination into a plant genome by utilizing an Agrobacterium tumefaciens Ti plasmid-based transformation system. The modified Ti plasmid is then used to transform recipient plant cells. Also provided are the plants and tissues produced by this method and bacterial strains, plasmids, and vectors useful for execution of this invention.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding δ-endotoxins having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, expression cassettes, and transformed microorganisms and plants comprising a nucleic acid of the invention. These compositions find use in methods for controlling pests, especially plant pests.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

The present invention provides insecticidal polypeptides related to shuffled Bacillus thuringiensis Cry1 polypeptides. Nucleic acids encoding the polypeptides of the invention are also provided. Methods for using the polypeptides and nucleic acids of the invention to enhance resistance of plants to insect predation are encompassed.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

The invention discloses a charcoal-based microbial soil conditioner. The charcoal-based microbial soil conditioner comprises, by weight, 80-85 parts of charcoal, 10-13 parts of a composite microbial flora and 1-2 parts of an oxidized starch adhesive, and the composite microbial flora comprises photosynthetic bacteria, actinomyces, lactic acid bacteria, microzyme, Bacillus subtilis and Bacillus thuringiensis. The invention also discloses a preparation method of the charcoal-based microbial soil conditioner. The combined effect of charcoal and microbes alleviates soil acidification, reduces the unit weight of soil and increases the permeability and the water content of soil; soil organic matter formation is promoted to improve the validity of mineral elements; and the abundance of microbes at the rhizosphere of crops is cultivated to improve the rhizospheric environment of plants in order to make pathogen microbes difficultly grow and breed, so the insect diseases of crops is reduced, and soil heavy metal pollution is alleviated and restored.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

Disclosed are novel synthetically-modified B. thuringiensis chimeric crystal proteins having improved insecticidal activity against coleopteran, dipteran and lepidopteran insects. Also disclosed are the nucleic acid segments encoding these novel peptides. Methods of making and using these genes and proteins are disclosed as well as methods for the recombinant expression, and transformation of suitable host cells. Transformed host cells and transgenic plants expressing the modified endotoxin are also aspects of the invention.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

The invention discloses a compound probiotics preparation for biological organic fertilizers and a preparation method thereof. The preparation method comprises the following steps: performing activated culture of 14 kinds of probiotics, including Pseudomona putida, Streptomyces violovariabilis, Rhizobium sp., Azotobacter chroococcum, Bacillus subtilis, Bacillus amyloliquefaciens, Bacillus cereus, Bacillus subtilis, Bacillus weihenstephanensis, Bacillus mucilaginosus, Bacillus thuringiensis, Saccharomyces rouxii, Streptomyces griseus var. ferrugineus, and Streptomyces microflavus; performing mixed inoculation at a proportion of 1% to 5% to a 500 mL liquid seed culture medium; then performing amplification culture; and finally delivering the strains to a 500 mL fermentor and fermenting for 3 to 5 days under such conditions that the temperature ranges from 28 DEG C to 30 DEG C and the rotational speed is 200 rpm, to obtain a liquid-state compound probiotics preparation. The compound probiotics preparation for biological organic fertilizers, provided by the invention, plays important roles of taking advantage of microbial ecology, improving soil, promoting plant growth, improving the yield and disease resistance of crops and reducing the consumption of fertilizers.

Cotton cells are transformed with a chimeric gene that expresses in the cells a polypeptide having substantially the insect toxicity properties of Bacillus thuringiensis crystal protein. The transformed cells are regenerated into plants that are toxic to the larvae of lepidopteran insects.

The invention relates to a liquid compound biological fertilizer with long shelf life as well as a preparation method and application thereof. The liquid compound biological bacterial fertilizer comprises the following components in parts by weight: 2-5 parts of microbial thalli, 80-130 parts of compound nutrients, 10-30 parts of thalli protective agents and 0.5-3.0 parts of additives, wherein the microbial thalli is metered in terms of the wet weight of deposition obtained after fermentation liquor is centrifugalized, the microbial thalli include bacillus thuringiensis, bacillus megaterium, bacillus mucilaginosus and bacillus amyloliquefaciens, and the total viable count achieves 5*10<8>-5*10<9> / mL. The liquid compound biological bacterial fertilizer disclosed by the invention can be used as a biological pesticide, can be directly applied to the spraying of plant surfaces or applied to seed soaking, root irrigation, and the like, has the effects of insect resistance, phosphate solubilization, potassium solubilization, organic matter solubilization, pest resistance, and the like and is long in shelf life.

The invention relates to a compound microbial fertilizer and a preparation method thereof. The compound microbial fertilizer is prepared by mixing the following raw materials: yeast fermentation powder residues, an organic fertilizer, urea, K2SO4, NH4H2PO4, clay and a compound microbial agent; and the compound microbial agent is formed by fermentation of bacillus mucilaginosus, bacillus megaterium, bacillus cereus, bacillus thuringiensis, azotobacter chroococcum, pichia farinose and streptomyces microflavus. The compound microbial fertilizer disclosed by the invention has a variety of microbes with the effects of nitrogen fixation, phosphorus dissolution and potassium dissolution, can increase the nitrogen content in soil, improves the utilization rate of the chemical fertilizer, and is favorable for improving the structure of the soil and improving the yield and quality of crops; and simultaneously, the microbes in the fertilizer can produce a variety of metabolites, and the metabolites can effectively resist diseases and insect pests.

The invention discloses a composite microbial fertilizer containing polyglutamate high-producing strain, a preparation method and an application thereof, belonging to a field of the composite microbial fertilizers. The method comprises preparing Bacillus licheniformis, Bacillus megaterium, Bacillus thuringiensis and Bacillus amyloliquefaciens into bacteria liquid through liquid fermentation, and mixing with complex composite nutrients according to a mass ratio of 1 : 1 to obtain the high-efficiency liquid-state composite microbial fertilizer. On basis of a good phosphate dissolving capability of the Bacillus megaterium, a good pest-resistant capability of the bacillus thuringiensis and a good organic matter degrading capability of the Bacillus amyloliquefaciens, the fertilizer combines performances of excellent water retention, fertilizer retention and non pollution to the environment of gamma-polyglutamate produced by the Bacillus licheniformis in a high yield, so as to achieve a relatively good fertilizer efficiency and relatively few using amount of water, and thus form scientific and reasonable composite fertilizer match.

The invention discloses a compound microbial preparation and a preparation method thereof, and belongs to the fields of environmental protection and microbiology. The compound microbial preparation comprises EM bacteria, bacillus subtilis, beauveria bassiana and bacillus thuringiensis. According to the invention, the compound microbial preparation is prepared from complementary and antergic microbial strains. The compound microbial preparation has advantages of various kinds, reasonable compatibility, strong function, high economic benefit, and the like. The compound microbial preparation has good effects on biological deodorization and plant insect control.

The present invention relates to novel gene sequences encoding insecticidal proteins produced by Bacillus thuringiensis strains. Particularly, new chimeric genes encoding a Cry1C, Cry1B or Cry1D protein are provided which are useful to protect plants from insect damage. Also included herein are plant cells or plants comprising such genes and methods of making or using them, as well as plant cells or plants comprising one of such chimeric gene and at least one other of such chimeric genes.

The invention discloses a selenium-enriched microorganism preparation prepared from fermentation of a mixed bacterium liquid. The selenium-enriched microorganism preparation consists of the following raw materials: sodium selenite, complex trace elements, monopotassium phosphate, urine, peptone powder, NaCl, NaOH, NaNO3, NaCl, NH4Cl, K2HPO4, a beef extract, glucose, starch, calcium carbonate, amino acid, potassium nitride, ferrous sulfate, magnesium sulfate, trisodium citrate, a biochemical enzyme and a mixed bacterium liquid; and the mixed bacterium liquid is a mixed liquid of the bacterium liquid of bacillus amyloliquefaciens, the bacterium liquid of bacillus subtilis, the bacterium liquid of bacillus licheniformis, the bacterium liquid of bacillus thuringiensis and the bacterium liquid of bacillus mucilaginosus. The mixed bacterium liquid applicable to the selenium-enriched microorganism preparation disclosed by the invention is conducive to microorganisms in soil, and can be used for increasing the quantity of the microorganisms in the soil and enhancing the activity of the microorganisms in the soil. Due to vital activity and metabolite, the strain is an agricultural product which can cause special fertilizer effect, growth regulating effect, effect of prevention and control of plant diseases and pests, soil bioremediation and the like to crops.

A wettable powder of bacillus thuringiensis and beauveria bassiana relates to a mixed biopesticide, aiming to the shortcomings of prevention and treatment limitations of beauveria bassiana and bacillus thuringiensis (Bt), particularly poor quick-acting property of microbial pesticides. The wettable powder provided in the invention is prepared from the following components: 10-14wt.% of bacillus thuringiensis, 4-14wt.% of beauveria bassiana, 3-5wt..% of wetting agent, 10-20wt.% of dispersant, 0.1-0.5wt.% of ultraviolet protectant and 50-60 wt.% of carrier. The wettable powder of bacillus thuringiensis and beauveria bassiana provided in the invention is high in activity, stable in effect, long in persistence and convenient for use.

The invention discloses an environmentally-friendly efficient microbial compound fertilizer and a preparation method thereof. The environmentally-friendly efficient microbial compound fertilizer is prepared from chicken manure, cow dung, dried pond sludge powder, ground phosphate rock, edible fungi residues, furfural residues, a microbial agent, wheat bran, bean cakes, urea, ammonium nitrate, potassium humate, wheat straws, charcoal powder and traditional Chinese medicine residues, wherein the microbial agent is prepared from bacillus licheniformis, bacillus subtilis, bacillus mucilaginosus, bacillus megatherium, pseudomonas, bacillus thuringiensis, bacillus amyloliquefaciens and nitrogen-fixing bacteria. The edible fungi residues and the furfural residues are smashed and mixed with water, the microbial agent is added for fermentation, the Chinese traditional medicine residues are added to perform fermentation, then the rest of the raw materials are added, and the microbial compound fertilizer can be obtained by performing full mixing, granulation and drying. The raw materials used for the microbial compound fertilizer are all natural fertilizers and contain rich mineral substances, the damage of a soil acid-base structure and a nutritional ingredient structure caused by inorganic fertilizer application is avoided, and the environmentally-friendly efficient microbial compound fertilizer is greener and more environmentally-friendly.

The invention is suitable for the technical field of recycling organic wastes, and discloses a flora for recycling treatment of organic wastes. The flora for recycling treatment of organic wastes is efficiently compounded from a variety of bacteria such as bacillus subtilis, aspergillus niger, saccharomycete and soil flora, wherein the soil flora is extracted from the natural world and comprises three or more of bacillus megatherium, bacillus mucilaginosus, bacillus licheniformis, bacillus thuringiensis, bacillus laterosporus, bacillus mucilaginosus, streptomyces jingyangensis, mycorrhiza fungi, azotobacter vinelandii, photosynthetic bacteria, bacillus coagulans, aspergillus oryzae, and paecilomyces lilacinus. According to the flora for recycling treatment of organic wastes, disclosed by the invention, by virtue of mutual promotion, mutual coordination and combined action of a variety of beneficial microorganisms, the organic wastes are recycled, environmental pollution is reduced, and wastes are changed into valuables; and the flora for recycling treatment of organic wastes can be used for producing industrial fermentation products and improving the health and growth conditions of animals, and can also improve the current situation of serious soil hardening at present and improve soil quality, thereby promoting the rapid development of agricultural economics.