121 results about "Bacillus thuringiensis" patented technology

The invention belongs to the technical field of genetic engineering, and discloses a bacillus thuringiensis-sourced brown planthopper-resistant insecticidal protein and a coding gene and application thereof, the bacillus thuringiensis-sourced brown planthopper-resistant insecticidal protein is an insecticidal protein orf1715, the amino acid sequence is shown as SEQ ID NO: 1, and the nucleotide coding sequence of the gene is shown as SEQ ID NO: 2. The gene is well expressed in an escherichia coli system in a soluble form, and the purified protein has a remarkable specific insecticidal effect on rice pest brown planthopper, so that the gene can be applied to special prevention and control of brown planthopper, and a candidate gene resource is provided for cultivation of a new transgenic insect-resistant rice variety.

The invention provides bacillus thuringiensis for efficiently poisoning and killing plant parasitic nematodes and application of the bacillus thuringiensis, and belongs to the technical field of microorganisms. According to the invention, a strain of bacillus thuringiensis BMB-0181 capable of efficiently poisoning and killing plant parasitic nematodes is screened from soil, and the preservation number of the bacillus thuringiensis BMB-0181 is CCTCC (China Center for Type Culture Collection) NO: M 20242644. According to the present invention, the detection results of the strain fermentation broth prove that the trans-aconitic acid yield can be high, and the biological assay and the pot culture experiment prove that the strain has the high poisoning activity on the meloidogyne incognita, can effectively control the field planting of the meloidogyne incognita, and further has the certain growth promoting effect on the tomato; the strain disclosed by the invention can be developed into a novel biological nematode killing pesticide.

The present invention describes capsule compositions consisting of crosslinked chitosan, beta-glucan or alginate, and encapsulates an environmentally compatible insecticide, especially larvicidal agents such as essential oils or essential oil components or other ingestible larvicides such as Bacillus thuringiensis which are lethal to the larvae and adults of pest insects, especially mosquito larvae.

The invention relates to bacillus thuringiensis and application of the bacillus thuringiensis in luring adult fruit flies, the classification name of the bacillus thuringiensis is bacillus thuringiensis XLB2910, the bacillus thuringiensis is preserved in the China Center for Type Culture Collection on May 19, 2025, and the strain preservation number is CCTCC NO: M 20251097. The strain disclosed by the invention is subjected to shake culture and fermentation in a GYS culture medium, and the supernatant of the fermentation liquor shows efficient attraction activity on female and male adults of bactrocera cucurbitae and bactrocera minax, so that the strain has relatively high practical application value and can be used for preventing and treating bactrocera minax.

The invention provides a soil conditioner for improving soil hardening and a preparation method thereof, and relates to the technical field of soil conditioning. The soil conditioner is prepared by combining nano silicon dioxide, a straw retting material, a straw composite fermentation material, biochar, a water-retaining agent and modified starch. The straw composite fermented material is prepared by the following steps: crushing straws, mixing with sweet water, stirring, drying, carrying out microwave treatment, mixing with bacillus thuringiensis, fermenting, sterilizing, subsequently adding a composite microbial agent of bacillus subtilis, trichoderma and bacillus mucilaginosus, continuously fermenting, and drying. The modified starch is prepared by semi-gelatinizing corn starch and then carrying out microwave drying and shaping. According to the present invention, the defects in the prior art are overcome, the soil hardening phenomenon can be rapidly improved with the small addition amount, the soil porosity is increased, the activity and the diversity of the soil microorganisms are ensured, and the soil fertility is improved.

The invention belongs to the technical field of heavy metal pollution treatment, and relates to preparation of a weakly alkaline soil cadmium passivator and a method for passivating soil lead. The alkalescent soil cadmium passivating agent mainly comprises the following components in parts by weight: 20 to 30 parts of graphene oxide modified biochar, 5 to 10 parts of montmorillonite, 5 to 10 parts of bentonite and 5 to 10 parts of a microbial agent, the microbial agent is a composite microbial agent formed by mixing bacillus subtilis, bacillus amyloliquefaciens, bacillus thuringiensis and pseudomonas nitroreducens. The alkalescent soil cadmium passivator has a very good remediation effect on cadmium and lead in polluted soil, can remarkably improve the physicochemical properties of the cadmium and / or lead polluted soil, increase the content of organic matters in the soil, reduce the migration of the soil in the environment and reduce the content of cadmium in crops, and has a great market popularization value.

The invention belongs to the technical field of environmental engineering, and discloses a microbial agent for degrading malathion as well as a preparation method and application of the microbial agent. The microbial agent comprises the following raw materials: compound bacteria, a nutritional supplement and a biomass carrier material, wherein the compound bacteria comprise functional bacteria and synergistic bacteria, and the mass ratio of the functional bacteria to the synergistic bacteria is (1.2-1.8): 1; the functional bacteria comprise issatchenkia orientalis, nocardia, brevibacillus laterosporus and bacillus cereus; the synergistic bacteria comprise azospirillum brasilense, bacillus thuringiensis and lactobacillus buchneri. Functional bacteria with direct degradation capacity and synergistic bacteria with auxiliary functions are creatively compounded according to a specific proportion, meanwhile, the garlic straw powder is selected as a biomass carrier material, all the raw materials complement each other in function and have a synergistic effect, the degradation rate and thoroughness of malathion are remarkably improved, and the degradation efficiency of malathion is greatly improved. The defects of low degradation efficiency and poor environmental adaptability of a single strain are overcome.

The invention discloses a use of a polylactic acid active packaging film in the preservation of B. cerana. The polylactic acid active packaging film is prepared by using an Ag@MOF carrier to load a natural antibacterial agent, which is then fully mixed with polylactic acid and then melt-extrusion blow molding. The packaging film is used to package the B. cerana to achieve active packaging of the B. cerana, inhibit the growth of microorganisms, and extend the shelf life of the B. cerana. The preservation method has a simple process and is easy to operate. The materials used are biodegradable, reducing environmental pollution caused by packaging. The natural antibacterial agent, Litsea cubeba essential oil, is added to the preservative film, which can effectively inhibit the growth and reproduction of major spoilage bacteria isolated and identified from the B. cerana. The Ag@MOF is used to load the Litsea cubeba essential oil, which can effectively control the release rate of the Litsea cubeba essential oil, achieving long-lasting antibacterial and antioxidant purposes. The preservation method can effectively extend the storage period of the B. cerana.

The invention discloses a specific composition, a kit and a detection method, a specific primer group is designed and obtained, the identification accuracy of bacillus cereus, bacillus thuringiensis and bacillus mycoides can be remarkably improved through the primer group, and the detection time is shortened to 6 hours from 3-10 days required in the prior art. According to the detection method, the bacillus cereus, the bacillus thuringiensis and the bacillus mycoides in the food can be rapidly identified, so that the food-borne pathogenic bacterium bacillus cereus can be better distinguished from the three bacteria with extremely high homology, and technical support is provided for a supervision department to detect the food-borne pathogenic bacteria.

The invention relates to a promoter PinhA1 and application thereof in guiding efficient expression of functional genes. The nucleotide sequence of the promoter PinhA1 is as shown in SEQ ID NO: 1. The strong promoter PinhA1 can be used for efficiently guiding the expression of the cry1Ac gene in the bacillus thuringiensis delta cdsR mutant. According to the invention, a strain delta cdsR (PinhA1-1Ac) which is used for guiding cry1Ac expression by PinhA1 and generating a large number of regular bipyramid crystal proteins is constructed, and the Cry1Ac yield of the strain delta cdsR (PinhA1-1Ac) and the insecticidal activity of the strain delta cdsR (PinhA1-1Ac) on plutella xylostella larvae are equivalent to those of a wild strain HD73. Results prove that the strong promoter PinhA1 has a good application prospect in the aspect of constructing a bacillus thuringiensis engineering strain, and a new strategy is provided for improving crystal protein or other insecticides in future agricultural production.

The invention relates to a diptera adult insecticide, which contains an insecticidal functional component n-butyl alcohol. According to the invention, n-butyl alcohol which has an obvious poisoning effect on the adult cucurbita petiolatus is separated from a metabolite of a bacillus thuringiensis XLB2910 strain, and 2, 3, 5-trimethylpyrazine and benzothiazole which have an obvious poisoning effect on the adult aedes albopictus are also separated from the metabolite of the bacillus thuringiensis XLB2910 strain. Through poisoning concentration analysis of different components and a compound combination experiment, a series of pesticides for adult cucurbita petioles and adult aedes albopictus are formed, the prepared compound pesticide for cucurbita petioles comprises 2.5%-10% of 2, 3, 5-trimethylpyrazine, 2%-10% of benzothiazole and 5%-10% of n-butyl alcohol, and the prepared compound pesticide for aedes albopictus comprises 2.5%-10% of 2, 3, 5-trimethylpyrazine, 2%-10% of benzothiazole and 5%-10% of n-butyl alcohol. The composition comprises 0.5%-3% of 1, 3, 5-trimethylpyrazine, 0.05%-1.5% of benzothiazole and 1%-3% of n-butyl alcohol. The compound insecticide has a good insecticidal effect on cucurbita petiolatus adults and aedes albopictus adults, and is wide in raw material source and environment-friendly.

The invention relates to the field of gene engineering, in particular to application of an overexpressed perR gene to improvement of the biological manganese oxidation effect of bacillus thuringiensis. According to the invention, a strain Bt 97-27 is used as an original strain, and the strain can oxidize Mn (II), form spores and generate a biological membrane to resist external adverse environmental factors. The novel genetic engineering strain OEperR is successfully constructed through overexpression of the perR gene in the Bt 97-27 strain, compared with an original strain, the OEperR strain is remarkably improved in the aspects of manganese oxidation, manganese removal, biological membrane generation and the like, the culture and domestication periods of the strain are shortened, and the yield of the strain is increased. And the environmental adaptability and tolerance of the strain are greatly enhanced, a new way is opened up for the application of a microbial technology in the field of manganese polluted environment restoration, and an important basis is provided for deeply exploring how microorganisms respond to manganese elements and regulating and controlling the oxidation process of the manganese elements.

The invention discloses an integrated method for realizing sand prevention and stabilization and soil improvement based on coal gangue, which comprises the following specific steps: crushing the coal gangue, grading and screening, taking the coal gangue with the particle size of 0.1-10 mm, and adding bacillus thuringiensis and a compound additive into the coal gangue for activating treatment; the activated coal gangue is mixed with sand grains, then the coal gangue with the grain size being 10-100 mm is laid on the desert earth surface, and integrated treatment is completed; under the combined action of the bacillus thuringiensis and the compound additive, degradation of the coal gangue is accelerated to form a fine particle matrix, and organic matters contained in the coal gangue are synchronously degraded to generate humic acid; meanwhile, metabolites of the bacillus thuringiensis and biological enzymes can promote slow release of nutrient elements such as silicon, potassium and phosphorus in the coal gangue, so that the physical and chemical properties of desert sand grains are improved, and the dual purposes of sand prevention and stabilization and soil improvement are finally achieved by combining the surface laying effect of the coal gangue with the large particle size.

The invention relates to construction and application of an engineering bacterium for high yield of chitinase ChiA, in particular to a bacillus thuringiensis engineering bacterium HD73 delta cdsR (P5014-SPchiD-chiA) strain, and the preservation number of the bacillus thuringiensis engineering bacterium HD73 delta cdsR (P5014-SPchiD-chiA) strain is CGMCC (China General Microbiological Culture Collection Center) No.35677. The HD73 endogenous gene chiA gene is efficiently expressed in the bacillus thuringiensis delta cdsR, and the yield in a fermentation culture medium is far higher than that of a wild strain HD73. Compared with HD73 (P5014-SPchiD-chiA), the compound has a relatively strong inhibition effect on mycelial growth of fusarium graminearum and exserohilum turcicum, and shows a better effect on prevention and treatment of larvae of plutella xylostella compared with HD73 (P5014-SPchiD-chiA).

The application provides a Bacillus thuringiensis strain, and the preservation number of the Bacillus thuringiensis strain is GDMCC No. 67284. The lethal rate of the Bacillus thuringiensis strain provided by the application to second instar larvae of root-knot nematode reaches 98%, the control effect in a pot experiment reaches 60.07%, and the control effects on root rot and wilt are 66.79% and 63.23% respectively. The Bacillus thuringiensis strain is a bacillus isolated from rhizosphere soil, is safe to crops, and has control effects on root rot, wilt and root-knot nematode, and has the safety characteristics in agricultural application.

The present invention describes capsule compositions consisting of crosslinked chitosan, beta-glucan or alginate, and encapsulates an environmentally compatible ingestible larvicide, especially larvicidal agents such as essential oils or other ingestible larvicides such as Bacillus thuringiensis which are lethal to the larvae of pest insects, especially mosquito larvae.

The invention provides a beta-glucosidase mutant from Paenibacillus phyllosphaerae, and a method for efficiently preparing rare ginsenoside F2 by using an enzyme method and a whole-cell method, and particularly provides a beta-glucosidase mutant from Paenibacillus phyllosphaerae and a method for efficiently preparing rare ginsenoside F2 by using a beta-glucosidase mutant from Paenibacillus phyllosphaerae. The method comprises the following steps: engineering-modifying beta-glucosidase by utilizing computer-aided design, constructing a mutant library, carrying out primary screening and secondary screening to obtain two high-activity mutants Q7D and G189A, constructing a combined mutant Q7D / G189A, transforming the combined mutant Q7D / G189A into genetically engineered bacterium escherichia coli for expression, and producing F2 by utilizing a whole-cell method. Finally, the highest yield of 18.01 g / L is achieved after the reaction is carried out for 10 h in a 5 L fermentation tank and is 6.82 times that of a shake flask, and the purity of F2 reaches up to 82.20% after the reaction is carried out for 24 h finally. The invention not only provides an efficient solution for large-scale production of rare saponin F2, but also provides a transferable framework for designing and engineering diversified glycoside hydrolase in customized biological catalysis.

The present invention provides a strain of Bacillus thuringiensis that is highly effective in killing plant parasitic nematodes and its application, belonging to the field of microbial technology. The present invention screens and obtains a strain of Bacillus thuringiensis (Bacillus thuringiensis) BMB-0181 that is highly effective in killing plant parasitic nematodes from soil, and the deposit number is CCTCC NO:M 20242644. The fermentation liquid of the strain is used to detect and confirm that it can produce high trans-aconitic acid. It is confirmed by bioassay and potted plant experiments that it has high toxicity to southern root-knot nematodes, can effectively control the colonization of southern root-knot nematodes, and has a certain growth-promoting effect on tomatoes. The strain of the present invention can be developed into a new biological nematode pesticide.

The invention relates to construction and application of an engineering bacterium for high yield of chitinase ChiD, in particular to a bacillus thuringiensis engineering bacterium deltacdsR (P5014-chiD) strain, and the preservation number of the bacillus thuringiensis engineering bacterium deltacdsR (P5014-chiD) strain is GGMCC No.35124. The HD73 endogenous gene chiD gene is efficiently expressed in the bacillus thuringiensis delta cdsR, and the yield in a fermentation culture medium is far higher than that of a wild strain HD73. Compared with HD73 (P5014-chiD), the compound has a relatively strong inhibition effect on mycelial growth of fusarium graminearum and exserohilum turcicum, and shows a better effect on prevention and treatment of larvae of plutella xylostella compared with the HD73 (P5014-chiD).

The invention belongs to the technical field of agricultural microbial preparations. The rice thuringiensis granule suspending agent comprises a bacillus thuringiensis concentrated solution and a rice leaf folder granule virus suspension according to a volume ratio of 1000: (1-2), in the bacillus thuringiensis concentrated solution, the biological value of bacillus thuringiensis is greater than or equal to 16000 IU / ml; in the rice leaf roller granulovirus suspension, the rice leaf roller granulovirus content is greater than or equal to 100000 OB / ml. The invention is used for solving the technical problem that the existing common biopesticide dosage form cannot meet the unmanned flight prevention requirement. The invention further discloses a preparation method of the rice thuryun longitudinal particle suspending agent. The invention further discloses an application method of the rice thuryun longitudinal particle suspending agent.

The invention discloses a carbon-based biological liquid organic water-soluble fertilizer as well as a preparation method and application thereof, and belongs to the technical field of biological organic fertilizers. The water-soluble fertilizer comprises the following raw materials in parts by weight: 15-25 parts of a seaweed extract, 10-15 parts of potassium fulvate, 5-10 parts of a compound microbial agent, 3-5 parts of potassium dihydrogen phosphate, 2-4 parts of potassium nitrate, 2-3 parts of urea, 2-3 parts of calcium nitrate, 1-2 parts of magnesium sulfate heptahydrate, 1-1.5 parts of a trace element-containing substance, 1-2 parts of sodium dodecyl benzene sulfonate and 0.5-1 part of potassium sorbate. The total amount of the components is 100 parts. According to the invention, three strains such as pseudomonas sevivaldi, bacillus pumila and bacillus thuringiensis are screened to play roles in three dimensions of promoting growth, improving rhizosphere environment and preventing and treating diseases and insect pests respectively, and are combined with organic components such as seaweed extract and potassium fulvate in the fertilizer and other components, so that the fertilizer has a good application prospect. A comprehensive system of nutrition, growth promotion, disease resistance and quality improvement is formed, and the yield and quality of grapes are remarkably improved.

The invention provides a Cry53-like insecticidal protein and application thereof in prevention and treatment of diptera pests, and belongs to the technical field of biopesticides, the Cry53-like insecticidal protein is separated from bacillus thuringiensis, and the amino acid sequence of the Cry53-like insecticidal protein is shown as SEQ ID NO.2. The invention further provides a preparation method of the Cry53-like insecticidal protein. Compared with the reported Cry insecticidal protein, the Cry53-like insecticidal protein has the advantages that the highest homology is only 38%, the Cry53-like insecticidal protein is determined to be a novel insecticidal protein, the Cry53-like insecticidal protein has advantages in prevention and treatment of diptera pests, the killing effect of the Cry53-like insecticidal protein on bradysia odoriphaga is obviously superior to that of other same-type Cry insecticidal proteins, and the Cry53-like insecticidal protein is a novel insecticidal protein. Therefore, the gene has application prospects in the fields of biopesticide creation, transgenic insect-resistant crop cultivation and the like.

The invention relates to the technical field of insecticides, in particular to an insecticide composition and a preparation method and application thereof.The insecticide composition is prepared from, by weight, 5-25 parts of neonicotinoid insecticides, 2-15 parts of 4-trifluoromethyl nicotinamide, 2-5 parts of microbial insecticides, 2-20 parts of botanical insecticides and 2-10 parts of sulfonated modified graphene nano powder. 5-15 parts of silicon dioxide-titanium oxide composite powder particles; the microbial insecticide is prepared from bacillus thuringiensis and metarhizium anisopliae; the botanical insecticide is prepared from azadirachtin, veratrine and celastrus angulatus. By matching and combining the raw materials, the prepared insecticide composition can improve the insecticidal effect while reducing the use of neonicotinoid insecticides, and reduces the influence on the farmland environment.

The invention provides an acne-removing external preparation and a preparation method thereof. The acne-removing external preparation comprises the following raw materials in parts by weight: 5-15 parts of an acne-removing composition and 50-95 parts of auxiliary materials, the acne-removing composition is obtained by sequentially carrying out enzymolysis and fermentation on traditional Chinese medicine raw materials, the traditional Chinese medicine raw materials comprise paris polyphylla, curcuma zedoary, rhizoma sparganii, rhizoma bletillae and selfheal in a mass ratio of (0.5-0.8): (1-3): (3-5): (6-8): A composite enzymolysis agent is used for enzymolysis, and the composite enzymolysis agent comprises inulase, beta-amylase, streptavidin and hemicellulase according to the mass ratio of 1: (1.5-3): (5-6.5): (8-10); a composite leavening agent is used for fermentation, and the composite leavening agent comprises citrobacter, bifidobacterium longum, bacillus thuringiensis and bacillus xylinum according to the mass ratio of (0.1-0.3): (1-3): (0.5-0.8): 1. The acne-removing external preparation prepared by the invention has good safety and acne-removing effect.

The present invention provides a planting method for promoting the early survival and growth of Panax notoginseng, and relates to the technical field of Panax notoginseng planting. The planting method mainly comprises the following steps: disinfecting and hormone-treating the Panax notoginseng seeds in the early stage, fumigating them with a certain concentration of cymene, and then watering them with a sterilized solution of pine needles and corn fermented with Bacillus thuringiensis after sowing. The present invention overcomes the shortcomings of the existing technology, can effectively improve the early emergence efficiency of Panax notoginseng, ensure uniform emergence of seedlings, promote root growth of Panax notoginseng seedlings, increase the vigorous seedling rate of Panax notoginseng seedlings, and comprehensively improve the economic benefits of Panax notoginseng planting.

A pest control composition comprising humic acid, Bacillus thuringiensis, and water wherein the humic acid has a phenolic group concentration of from 0.19 mmol / g of the humic acid to 1.30 mmol / g of the humic acid.

A pest control composition comprising humic acid, a polymer containing 20% to 100% by weight of monomer structural units derived from a monomer having a weight average molecular weight of 10,000 to 30,000 daltons and a logP of 2.0 to 6.0, Bacillus thuringiensis, and water.

The present invention relates to the field of genetic engineering, and in particular to the application of overexpressing the perR gene in improving the biological manganese oxidation of Bacillus thuringiensis. The present invention uses the strain Bt 97-27 as the starting strain, which is capable of oxidizing Mn (II), forming spores and producing biofilms to resist adverse external environmental factors. The present invention successfully constructs a new genetically engineered strain OEperR by overexpressing the perR gene in the Bt 97-27 strain. Compared with the starting strain, the OEperR strain achieves significant improvements in manganese oxidation, manganese removal and biofilm formation. It not only shortens the culture and acclimation cycle of the strain, but also greatly enhances the environmental adaptability and tolerance of the strain, opens up a new way for the application of microbial technology in the field of manganese-contaminated environmental remediation, and provides an important basis for in-depth exploration of how microorganisms respond to manganese and regulate its oxidation process.

The invention discloses a hydrogel and probiotic compounded microbial inoculum composition and a vegetable planting matrix, a three-dimensional gel carrier is used for embedding a microbial inoculum, the carrier integrates mechanical properties and thallus protection and can maintain flora activity for a long time, bacillus thuringiensis has a low-oxygen survival characteristic after being subjected to Fnr enhanced expression, and the microbial inoculum composition has the advantages that the microbial inoculum composition has a low-oxygen survival characteristic after being subjected to Fnr enhanced expression; the microbial agent composition can survive for a long time in a low-oxygen environment of a carrier embedding environment, and is cooperated with bacillus cereus, azotobacter chroococcum and bacillus megatherium to promote the growth of plants such as vegetables and the like and directionally kill pathogenic microorganisms such as root-knot nematode. Corresponding pests can be killed in a green and targeted manner, the growth of plants is promoted, and the yield is increased.

The invention discloses a fertilizer prepared from fructus psoraleae waste and a fermentation product of Citrus chachiensis Hance, and belongs to the technical field of agricultural fertilizers. The fertilizer prepared from the fermentation product of the fructus psoraleae waste and the citrus reticulata blanco mainly comprises the following raw material components in parts by mass: 30-40 parts of the fructus psoraleae waste, 90-110 parts of citrus reticulata blanco pulp, 7.52-7.92 parts of a complex microbial inoculant, 20-40 parts of an auxiliary material and 0.4 part of urea, the complex microbial inoculant comprises any one or a combination of at least two of bacillus subtilis, bacillus thuringiensis, citrobacter, bacillus cereus and trichoderma; the auxiliary materials comprise any one or a combination of at least two of rice hull powder, peanut bran, bean dregs, sawdust, wheat bran, mushroom dregs and traditional Chinese medicine residues.