35 results about "Bacillus thuringiensis" patented technology

The invention relates to a diptera adult insecticide, which contains an insecticidal functional component n-butyl alcohol. According to the invention, n-butyl alcohol which has an obvious poisoning effect on the adult cucurbita petiolatus is separated from a metabolite of a bacillus thuringiensis XLB2910 strain, and 2, 3, 5-trimethylpyrazine and benzothiazole which have an obvious poisoning effect on the adult aedes albopictus are also separated from the metabolite of the bacillus thuringiensis XLB2910 strain. Through poisoning concentration analysis of different components and a compound combination experiment, a series of pesticides for adult cucurbita petioles and adult aedes albopictus are formed, the prepared compound pesticide for cucurbita petioles comprises 2.5%-10% of 2, 3, 5-trimethylpyrazine, 2%-10% of benzothiazole and 5%-10% of n-butyl alcohol, and the prepared compound pesticide for aedes albopictus comprises 2.5%-10% of 2, 3, 5-trimethylpyrazine, 2%-10% of benzothiazole and 5%-10% of n-butyl alcohol. The composition comprises 0.5%-3% of 1, 3, 5-trimethylpyrazine, 0.05%-1.5% of benzothiazole and 1%-3% of n-butyl alcohol. The compound insecticide has a good insecticidal effect on cucurbita petiolatus adults and aedes albopictus adults, and is wide in raw material source and environment-friendly.

The invention discloses an integrated method for realizing sand prevention and stabilization and soil improvement based on coal gangue, which comprises the following specific steps: crushing the coal gangue, grading and screening, taking the coal gangue with the particle size of 0.1-10 mm, and adding bacillus thuringiensis and a compound additive into the coal gangue for activating treatment; the activated coal gangue is mixed with sand grains, then the coal gangue with the grain size being 10-100 mm is laid on the desert earth surface, and integrated treatment is completed; under the combined action of the bacillus thuringiensis and the compound additive, degradation of the coal gangue is accelerated to form a fine particle matrix, and organic matters contained in the coal gangue are synchronously degraded to generate humic acid; meanwhile, metabolites of the bacillus thuringiensis and biological enzymes can promote slow release of nutrient elements such as silicon, potassium and phosphorus in the coal gangue, so that the physical and chemical properties of desert sand grains are improved, and the dual purposes of sand prevention and stabilization and soil improvement are finally achieved by combining the surface laying effect of the coal gangue with the large particle size.

The application provides a Bacillus thuringiensis strain, and the preservation number of the Bacillus thuringiensis strain is GDMCC No. 67284. The lethal rate of the Bacillus thuringiensis strain provided by the application to second instar larvae of root-knot nematode reaches 98%, the control effect in a pot experiment reaches 60.07%, and the control effects on root rot and wilt are 66.79% and 63.23% respectively. The Bacillus thuringiensis strain is a bacillus isolated from rhizosphere soil, is safe to crops, and has control effects on root rot, wilt and root-knot nematode, and has the safety characteristics in agricultural application.

The present invention describes capsule compositions consisting of crosslinked chitosan, beta-glucan or alginate, and encapsulates an environmentally compatible ingestible larvicide, especially larvicidal agents such as essential oils or other ingestible larvicides such as Bacillus thuringiensis which are lethal to the larvae of pest insects, especially mosquito larvae.

The invention provides a beta-glucosidase mutant from Paenibacillus phyllosphaerae, and a method for efficiently preparing rare ginsenoside F2 by using an enzyme method and a whole-cell method, and particularly provides a beta-glucosidase mutant from Paenibacillus phyllosphaerae and a method for efficiently preparing rare ginsenoside F2 by using a beta-glucosidase mutant from Paenibacillus phyllosphaerae. The method comprises the following steps: engineering-modifying beta-glucosidase by utilizing computer-aided design, constructing a mutant library, carrying out primary screening and secondary screening to obtain two high-activity mutants Q7D and G189A, constructing a combined mutant Q7D / G189A, transforming the combined mutant Q7D / G189A into genetically engineered bacterium escherichia coli for expression, and producing F2 by utilizing a whole-cell method. Finally, the highest yield of 18.01 g / L is achieved after the reaction is carried out for 10 h in a 5 L fermentation tank and is 6.82 times that of a shake flask, and the purity of F2 reaches up to 82.20% after the reaction is carried out for 24 h finally. The invention not only provides an efficient solution for large-scale production of rare saponin F2, but also provides a transferable framework for designing and engineering diversified glycoside hydrolase in customized biological catalysis.

The invention relates to construction and application of an engineering bacterium for high yield of chitinase ChiD, in particular to a bacillus thuringiensis engineering bacterium deltacdsR (P5014-chiD) strain, and the preservation number of the bacillus thuringiensis engineering bacterium deltacdsR (P5014-chiD) strain is GGMCC No.35124. The HD73 endogenous gene chiD gene is efficiently expressed in the bacillus thuringiensis delta cdsR, and the yield in a fermentation culture medium is far higher than that of a wild strain HD73. Compared with HD73 (P5014-chiD), the compound has a relatively strong inhibition effect on mycelial growth of fusarium graminearum and exserohilum turcicum, and shows a better effect on prevention and treatment of larvae of plutella xylostella compared with the HD73 (P5014-chiD).

The invention provides a Cry53-like insecticidal protein and application thereof in prevention and treatment of diptera pests, and belongs to the technical field of biopesticides, the Cry53-like insecticidal protein is separated from bacillus thuringiensis, and the amino acid sequence of the Cry53-like insecticidal protein is shown as SEQ ID NO.2. The invention further provides a preparation method of the Cry53-like insecticidal protein. Compared with the reported Cry insecticidal protein, the Cry53-like insecticidal protein has the advantages that the highest homology is only 38%, the Cry53-like insecticidal protein is determined to be a novel insecticidal protein, the Cry53-like insecticidal protein has advantages in prevention and treatment of diptera pests, the killing effect of the Cry53-like insecticidal protein on bradysia odoriphaga is obviously superior to that of other same-type Cry insecticidal proteins, and the Cry53-like insecticidal protein is a novel insecticidal protein. Therefore, the gene has application prospects in the fields of biopesticide creation, transgenic insect-resistant crop cultivation and the like.

The invention relates to construction and application of RNase III deletion type bacillus thuringiensis (RNase III). The bacterial strain is obtained by taking a bacillus thuringiensis engineering bacterial strain DB delta 189 as an initial bacterial strain and knocking out an rnc gene by adopting a gene editing method. The strain realizes oral delivery of dsRNA and effective silencing of a target gene; meanwhile, the insecticidal effect of the Bt strain HD1 can be obviously enhanced. According to the invention, a new action mechanism is introduced while the traditional insecticidal advantages of Bt are reserved, so that the insecticidal effect of the Bt strain is improved, and the application prospect of Bt in comprehensive pest control is expanded.

This invention belongs to the field of biodegradation promoter technology, specifically relating to a green synthesis method for a biodegradation promoter of cyclic olefin polymers. The invention involves aerobic fermentation of a mixture of *Bacillus thuringiensis*, *Bacillus licheniformis*, and *Candida*. The fermented product contains abundant enzymes and bioactive agents, which are beneficial for promoting the degradation of cyclic olefin polymers. Furthermore, by using modified chitosan to immobilize the fermented product, microorganisms and their metabolites can be fixed on the chitosan surface, promoting the full release of active functions. In addition, the stability of the modified chitosan structure is improved through a cross-linking reaction. Therefore, the biodegradation promoter provided by this invention can not only promote the biodegradation of cyclic olefin polymers but also be used in the preparation of biodegradable products containing cyclic olefin polymers.

The invention belongs to the technical field of insect pest control, and particularly discloses a complex microbial inoculant for preventing and treating insect pests and application thereof.The complex microbial inoculant is prepared from, by weight, active ingredients and auxiliary materials, the active ingredients comprise 5-8 parts of bacillus thuringiensis, 4-6 parts of beauveria bassiana, 3-5 parts of metarhizium anisopliae, 2-4 parts of bacillus subtilis, 2-3 parts of bacillus amyloliquefaciens and 1-3 parts of paecilomyces lilacinus, the auxiliary materials comprise the following components in parts by weight: 1-2 parts of a surfactant, 0.5-1 part of a protective agent, 2-3 parts of a nutrient synergist and 30-40 parts of a carrier. According to the complex microbial inoculant, multiple functional microorganisms and synergistic components are reasonably matched, comprehensive and efficient prevention and control of scale insects, red spiders, aphids and fruit borers are achieved, and the complex microbial inoculant is environmentally friendly and safe to durian growth. The defects that durian pest control depends on chemical pesticides, and the control effect of microbial agents is single and unstable in the prior art are overcome.

The invention discloses a compound microbial agent rich in beta-glucan as well as a preparation method and application of the compound microbial agent, and belongs to the technical field of agricultural biology. According to the microbial agent, an aureobasidium pullulans-rhizobium pullulans-bacillus subtilis-bacillus thuringiensis quaternary synergistic system is constructed, specific auxiliary materials, carriers and a coating technology are supplemented, and under the drought stress condition, the microbial agent can remarkably promote the growth (plant height and biomass) of the pakchoi, so that the yield of the pakchoi is increased, and the yield of the pakchoi is increased. The yield of the pakchoi is increased, the quality is improved (the content of soluble sugar and protein is increased), the chlorophyll content and the photosynthetic efficiency are improved, the antioxidant enzyme activity is enhanced, cell membrane damage is reduced, the physical and chemical properties of soil are improved, and a new effective solution is provided for agricultural green drought resistance.

The invention belongs to the field of microbial agents and plant growth promotion, and particularly relates to bacillus altitudinis YN18 and application thereof in corn growth promotion. The preservation number of the bacillus altitudinis YN18 is CCTCC (China Center for Type Culture Collection) NO: M 20251694, and the bacillus altitudinis YN18 is preserved in the China Center for Type Culture Collection on July 24, 2025; the invention relates to a bacillus altitudinis YN18, which is classified and named as bacillus altitudinis YN18. According to the invention, a strain of bacillus altitudinis YN18 is obtained through separation for the first time, and the strain YN18 has good siderophore production and IAA secretion capabilities, and can significantly promote corn plant height, root length, fresh root weight and dry root weight; therefore, the method has a good application prospect. The bacillus altitudinis YN18 provided by the invention has a remarkable emission reduction effect on NO emission of corn plants, the actually measured emission reduction rate is up to 106.52%, and the bacillus altitudinis YN18 shows excellent greenhouse gas regulation potential.

The invention belongs to the technical field of microorganisms, and discloses bacillus thuringiensis BMB4196 with high yield of thurinins and application of the bacillus thuringiensis BMB4196. The preservation number of the strain is CCTCC M 2026098, the strain has a remarkable high-yield characteristic of the thuringiensis, and the yield of the thuringiensis can reach 22.52 g / L under the condition of a 50L fermentation tank, which is the highest yield level of the thuringiensis reported at present. The strain is suitable for large-scale fermentation production of the thuringiensis, can effectively reduce the production cost and improve the production efficiency, provides an important strain resource for industrial application and product development of the thuringiensis, and has a good application prospect.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera and Coleoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

The present application relates to a kind of harmless physical method and reagent for treating corn borer.The reagent includes the following mass percentage components: colloid 0.2%-0.6%, diatomite 1%-2%, Bacillus thuringiensis 0.2%-0.3%, Beauveria bassiana 0.1%-0.15%, azadirachtin 0.03%-0.1%, and the balance is water.The present application uses the viscous colloid formed by colloid to effectively occupy corn bell mouth and stick to the corn borer that crawls out to feed, achieving physical control;At the same time, Bacillus thuringiensis and Beauveria bassiana biological components are used to infect and kill the corn borer, azadirachtin plays a role in repelling and growth inhibition, and diatomite can physically pierce the insect skin, enhancing the control effect, and through the synergistic effect of multiple components, biological control is achieved.The present application creatively uses harmless physical method combined with biological components to control corn borer, not only avoiding pesticide residues, but also being more environmentally friendly and safe, and synergistically enhancing the control effect, reducing the worm boring rate.

This invention belongs to the field of pesticide technology, and relates to oxime derivatives containing a 3,4-dihydroisoquinoline structure, their preparation methods, and applications. This invention synthesizes a class of oxime derivatives containing a 3,4-dihydroisoquinoline structure by introducing an oxime functional group into the 3,4-dihydroisoquinoline structure. These oxime derivatives and their hydrochlorides exhibit inhibitory effects on various plant pathogens, especially on Ascomycota and Deuteromycota pathogenic fungi, such as *Pseudomonas aeruginosa*, *Pseudomonas macrantha*, *Sclerotinia sclerotiorum*, *Botrytis cinerea*, *Fusarium graminearum*, and *Bacillus thuringiensis*, demonstrating excellent inhibitory activity. Therefore, oxime derivatives containing a 3,4-dihydroisoquinoline structure hold promise for further development into highly efficient, safe, economical, and environmentally friendly green fungicides.

The invention discloses a green prevention and control method for sugarcane borers, and relates to the technical field of crop disease and insect pest prevention and control. The green prevention and control method comprises the following steps: (1) thoroughly cleaning a garden in time after harvesting sugarcanes; (2) when newly planted sugarcanes are planted or ratoon sugarcanes are subjected to ridge breaking and stump loosening, biopesticide granules and decomposed farmyard manure are applied in planting ditches or nearby sugarcane stumps, and earthing and watering are carried out; the biopesticide granules are granules prepared by mixing a bacillus thuringiensis microbial inoculum and an auxiliary agent, and (3) interplanting spring soybeans among the sugarcane rows every year, continuously interplanting summer soybeans among the sugarcane rows after the spring soybeans are harvested, and applying pesticides to the soybeans and the sugarcanes in different areas during the planting period of the spring soybeans and the summer soybeans. According to the method, the bacillus thuringiensis fungicide, the auxiliaries and the decomposed farmyard manure are compounded for soil application, underground stem borers can be efficiently inhibited and killed, harm of sugarcane in the seedling stage is greatly reduced, meanwhile, the prevention and control effect of the natural enemy trichogramma can be exerted to the maximum extent through partitioned pesticide application, and the precise and efficient prevention and control effect is achieved.

The invention provides a rapid degradation process for a degradable express green plastic package. The rapid degradation process comprises the following steps: 1, classifying raw materials, and then crushing and weighing the raw materials; 2, preparing culture soil: mixing vermiculite, base soil, water and nutrient substances to prepare the culture soil; 3, preparation of culture bacteria: proportioning aspergillus niger, Penicillium Polish and Bacillus thuringiensis according to a certain ratio, and adjusting the concentration; 4, uniformly mixing: feeding the crushed plastic, the culture soil and the culture bacteria according to the addition amount of the volume of the reaction container, and uniformly mixing; 5, the mixture is put into a reaction container, air is supplemented while stirring, the air is CO2-removed gas, the temperature in the reaction container is controlled to be 15-55 DEG C, the water content of a reaction mixture is controlled to be 40-60%, and the pH value is controlled to be 7.0-9.0. The method has the beneficial effects that the degradable plastic package is degraded by adopting the microbial flora and the culture soil, and the method has the advantages of low cost, simplicity in operation, low energy consumption, high degradation efficiency and the like. And the reaction conditions are mild, so that the process is more environment-friendly and has industrial feasibility.

This invention discloses a method for preparing a bidirectional fermentation extract of *Bacillus thuringiensis* and traditional Chinese medicine, and the application of this extract in inhibiting plant pathogenic fungi, belonging to the fields of microbial fermentation technology and biopesticides. This invention significantly enhances the antibacterial activity of the extract through bidirectional fermentation of *Bacillus thuringiensis* and traditional Chinese medicine, achieving an inhibition rate of up to 100%, comparable to the positive control carbendazim at the same concentration. The active ingredients are enhanced: the content of total terpenes, total polyphenols, and total flavonoids is significantly increased after fermentation. It is green and safe: derived from natural traditional Chinese medicine and fungi, with no chemical residues, and is environmentally friendly.

The disclosure provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidopteran and Coleopteran. Particular embodiments of the disclosure provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

The invention relates to a bacillus thuringiensis engineering bacterium strain DB [delta] 189 and application of the bacillus thuringiensis engineering bacterium strain DB [delta] 189. According to the invention, LM-DB is used as an initial strain, and a chassis strain DB [delta] 189 is constructed for the first time by gradually knocking out a plurality of insecticidal genes (such as cryBP1, cry41Ca1, cry69Aa-like, nt32-like, mpp 74Aa1, mpp 45Ba1 and cry32-like) of the LM-DB. The strain has stable growth characteristics, excellent foreign protein expression performance and relatively high environmental tolerance. The strain not only can efficiently express insecticidal proteins (Cry1Ab and Vip3A) of a Bt source, but also can realize efficient co-expression of metabolic enzymes (Ech and Fcs) of a non-Bt source in cells, so that efficient bioconversion from ferulic acid to vanillin is realized. The invention lays a foundation for functional transformation of Bt and application of Bt in the fields of synthetic biology and biological manufacturing.

The invention relates to identification and characterization of recombinant DNA and polypeptides for specific Bt toxin receptors. In particular, the Bt toxin receptors of the invention include those derived from the Lepidopteran super family including the species Trichoplusiani ni, Pseudoplusia includens, Helicoverpa zea, and Spodoptera frugiperda. The receptors of the invention further include those derived from the Coleopteran super family and particularly from the species Diabrotica virgifera virgifera. The recombinant DNA and polypeptides so provided are useful in the identification and design of novel Bt toxin receptor ligands including novel or improved insecticidal toxins for use in a variety of agricultural applications. Materials and methods for identifying novel toxins are also disclosed herein. The invention also provides methods for selecting toxins to combine to control insect populations by manipulating Bt toxin receptor.

The invention relates to an insecticidal protein Cif and an expression vector and application thereof. The three-dimensional structure of the insecticidal protein Cof comprises two structural domains, and the structural domain I is composed of alpha helixes and located at the 16-187 sites of an amino acid sequence; the connecting domain consists of an alpha helix and three beta folds, and is positioned at the 188-285 sites of the amino acid sequence; and the structural domain II is formed by beta folding and is located at 286-426 sites of the amino acid sequence. The insecticidal protein Cof is a novel non-Cry protein, and can be effectively expressed in bacillus thuringiensis after sequence optimization. Experiments prove that the P5014 can guide expression of the Cif protein and has a poisoning effect on spodoptera frugiperda.

This invention discloses a carbon-based biological liquid organic water-soluble fertilizer, its preparation method, and its application, belonging to the field of bio-organic fertilizer technology. The water-soluble fertilizer of this invention comprises the following raw materials in parts by weight: 15-25 parts seaweed extract, 10-15 parts potassium humate, 5-10 parts compound microbial inoculant, 3-5 parts potassium dihydrogen phosphate, 2-4 parts potassium nitrate, 2-3 parts urea, 2-3 parts calcium nitrate, 1-2 parts magnesium sulfate heptahydrate, 1-1.5 parts trace element substances, 1-2 parts sodium dodecylbenzenesulfonate, 0.5-1 part potassium sorbate, 0.5-1 part organosilicon surfactant, and water to a total of 100 parts. This invention screens three strains of Pseudomonas sevivar, Bacillus microepiploicus, and Bacillus thuringiensis, which function in three dimensions: promoting growth, improving the rhizosphere environment, and preventing and controlling pests and diseases. These strains are combined with organic components such as seaweed extract and potassium humate in fertilizers, as well as other components, to form a comprehensive system of "nutrition + growth promotion + disease resistance + quality improvement", which significantly improves the yield and quality of grapes.

The disclosure provides nucleic acids, and variants and fragments thereof, derived from strains of Bacillus thuringiensis encoding variant polypeptides having increased pesticidal activity against insect pests, including Lepidoptera and Coleopteran. Particular embodiments of the disclosure provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

This invention discloses an integrated method for sand control and soil improvement based on coal gangue. The specific steps are as follows: After crushing and grading the coal gangue, coal gangue with a particle size of 0.1~10 mm is selected and activated by adding Bacillus thuringiensis and compound additives. The activated coal gangue is mixed with sand particles, and then coal gangue with a particle size of 10~100 mm is laid on the desert surface to complete the integrated treatment. Under the combined action of Bacillus thuringiensis and compound additives, the coal gangue is accelerated to degrade into a fine-particle matrix, and the organic matter contained therein is simultaneously degraded to generate humic acid. At the same time, the metabolites and bioenzymes of Bacillus thuringiensis can promote the slow release of nutrients such as silicon, potassium, and phosphorus in the coal gangue, thereby improving the physicochemical properties of desert sand particles. Combined with the surface laying effect of coarse-particle coal gangue, the dual purpose of sand control and soil improvement is finally achieved.

The invention discloses application of thuringirin in prevention and treatment of cotton aphid, green peach aphid, armyworm and soybean cyst nematode, and belongs to the field of microbial pesticides. The invention discovers that the thurinin has the biological activity of killing cotton aphid, green peach aphid, mythimna separata and soybean cyst nematode for the first time, has a wide application prospect, and can be developed into a novel environment-friendly biological control agent.

The invention relates to bacillus thuringiensis KHWY-1 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.33400. The strain is used for fermenting feathers for 20 hours under the conditions that the temperature is 25 DEG C and the pH value is 8, the soluble protein content reaches 691.37 mu g / mL, and the feather degradation rate reaches 90% or above. The invention also relates to a new application of the bacillus thuringiensis KHWY-1 in degrading the feather fermentation filtrate of the white feather chicken as a cosmetic raw material, and the additional value of feather degradation products is improved.