65 results about "Bacillus thuringiensis" patented technology

The invention relates to bacillus thuringiensis and application of the bacillus thuringiensis in luring adult fruit flies, the classification name of the bacillus thuringiensis is bacillus thuringiensis XLB2910, the bacillus thuringiensis is preserved in the China Center for Type Culture Collection on May 19, 2025, and the strain preservation number is CCTCC NO: M 20251097. The strain disclosed by the invention is subjected to shake culture and fermentation in a GYS culture medium, and the supernatant of the fermentation liquor shows efficient attraction activity on female and male adults of bactrocera cucurbitae and bactrocera minax, so that the strain has relatively high practical application value and can be used for preventing and treating bactrocera minax.

The invention provides a soil conditioner for improving soil hardening and a preparation method thereof, and relates to the technical field of soil conditioning. The soil conditioner is prepared by combining nano silicon dioxide, a straw retting material, a straw composite fermentation material, biochar, a water-retaining agent and modified starch. The straw composite fermented material is prepared by the following steps: crushing straws, mixing with sweet water, stirring, drying, carrying out microwave treatment, mixing with bacillus thuringiensis, fermenting, sterilizing, subsequently adding a composite microbial agent of bacillus subtilis, trichoderma and bacillus mucilaginosus, continuously fermenting, and drying. The modified starch is prepared by semi-gelatinizing corn starch and then carrying out microwave drying and shaping. According to the present invention, the defects in the prior art are overcome, the soil hardening phenomenon can be rapidly improved with the small addition amount, the soil porosity is increased, the activity and the diversity of the soil microorganisms are ensured, and the soil fertility is improved.

The invention belongs to the technical field of environmental engineering, and discloses a microbial agent for degrading malathion as well as a preparation method and application of the microbial agent. The microbial agent comprises the following raw materials: compound bacteria, a nutritional supplement and a biomass carrier material, wherein the compound bacteria comprise functional bacteria and synergistic bacteria, and the mass ratio of the functional bacteria to the synergistic bacteria is (1.2-1.8): 1; the functional bacteria comprise issatchenkia orientalis, nocardia, brevibacillus laterosporus and bacillus cereus; the synergistic bacteria comprise azospirillum brasilense, bacillus thuringiensis and lactobacillus buchneri. Functional bacteria with direct degradation capacity and synergistic bacteria with auxiliary functions are creatively compounded according to a specific proportion, meanwhile, the garlic straw powder is selected as a biomass carrier material, all the raw materials complement each other in function and have a synergistic effect, the degradation rate and thoroughness of malathion are remarkably improved, and the degradation efficiency of malathion is greatly improved. The defects of low degradation efficiency and poor environmental adaptability of a single strain are overcome.

The invention relates to a diptera adult insecticide, which contains an insecticidal functional component n-butyl alcohol. According to the invention, n-butyl alcohol which has an obvious poisoning effect on the adult cucurbita petiolatus is separated from a metabolite of a bacillus thuringiensis XLB2910 strain, and 2, 3, 5-trimethylpyrazine and benzothiazole which have an obvious poisoning effect on the adult aedes albopictus are also separated from the metabolite of the bacillus thuringiensis XLB2910 strain. Through poisoning concentration analysis of different components and a compound combination experiment, a series of pesticides for adult cucurbita petioles and adult aedes albopictus are formed, the prepared compound pesticide for cucurbita petioles comprises 2.5%-10% of 2, 3, 5-trimethylpyrazine, 2%-10% of benzothiazole and 5%-10% of n-butyl alcohol, and the prepared compound pesticide for aedes albopictus comprises 2.5%-10% of 2, 3, 5-trimethylpyrazine, 2%-10% of benzothiazole and 5%-10% of n-butyl alcohol. The composition comprises 0.5%-3% of 1, 3, 5-trimethylpyrazine, 0.05%-1.5% of benzothiazole and 1%-3% of n-butyl alcohol. The compound insecticide has a good insecticidal effect on cucurbita petiolatus adults and aedes albopictus adults, and is wide in raw material source and environment-friendly.

The invention discloses an integrated method for realizing sand prevention and stabilization and soil improvement based on coal gangue, which comprises the following specific steps: crushing the coal gangue, grading and screening, taking the coal gangue with the particle size of 0.1-10 mm, and adding bacillus thuringiensis and a compound additive into the coal gangue for activating treatment; the activated coal gangue is mixed with sand grains, then the coal gangue with the grain size being 10-100 mm is laid on the desert earth surface, and integrated treatment is completed; under the combined action of the bacillus thuringiensis and the compound additive, degradation of the coal gangue is accelerated to form a fine particle matrix, and organic matters contained in the coal gangue are synchronously degraded to generate humic acid; meanwhile, metabolites of the bacillus thuringiensis and biological enzymes can promote slow release of nutrient elements such as silicon, potassium and phosphorus in the coal gangue, so that the physical and chemical properties of desert sand grains are improved, and the dual purposes of sand prevention and stabilization and soil improvement are finally achieved by combining the surface laying effect of the coal gangue with the large particle size.

The invention relates to construction and application of an engineering bacterium for high yield of chitinase ChiA, in particular to a bacillus thuringiensis engineering bacterium HD73 delta cdsR (P5014-SPchiD-chiA) strain, and the preservation number of the bacillus thuringiensis engineering bacterium HD73 delta cdsR (P5014-SPchiD-chiA) strain is CGMCC (China General Microbiological Culture Collection Center) No.35677. The HD73 endogenous gene chiA gene is efficiently expressed in the bacillus thuringiensis delta cdsR, and the yield in a fermentation culture medium is far higher than that of a wild strain HD73. Compared with HD73 (P5014-SPchiD-chiA), the compound has a relatively strong inhibition effect on mycelial growth of fusarium graminearum and exserohilum turcicum, and shows a better effect on prevention and treatment of larvae of plutella xylostella compared with HD73 (P5014-SPchiD-chiA).

The application provides a Bacillus thuringiensis strain, and the preservation number of the Bacillus thuringiensis strain is GDMCC No. 67284. The lethal rate of the Bacillus thuringiensis strain provided by the application to second instar larvae of root-knot nematode reaches 98%, the control effect in a pot experiment reaches 60.07%, and the control effects on root rot and wilt are 66.79% and 63.23% respectively. The Bacillus thuringiensis strain is a bacillus isolated from rhizosphere soil, is safe to crops, and has control effects on root rot, wilt and root-knot nematode, and has the safety characteristics in agricultural application.

The present invention describes capsule compositions consisting of crosslinked chitosan, beta-glucan or alginate, and encapsulates an environmentally compatible ingestible larvicide, especially larvicidal agents such as essential oils or other ingestible larvicides such as Bacillus thuringiensis which are lethal to the larvae of pest insects, especially mosquito larvae.

The invention provides a beta-glucosidase mutant from Paenibacillus phyllosphaerae, and a method for efficiently preparing rare ginsenoside F2 by using an enzyme method and a whole-cell method, and particularly provides a beta-glucosidase mutant from Paenibacillus phyllosphaerae and a method for efficiently preparing rare ginsenoside F2 by using a beta-glucosidase mutant from Paenibacillus phyllosphaerae. The method comprises the following steps: engineering-modifying beta-glucosidase by utilizing computer-aided design, constructing a mutant library, carrying out primary screening and secondary screening to obtain two high-activity mutants Q7D and G189A, constructing a combined mutant Q7D / G189A, transforming the combined mutant Q7D / G189A into genetically engineered bacterium escherichia coli for expression, and producing F2 by utilizing a whole-cell method. Finally, the highest yield of 18.01 g / L is achieved after the reaction is carried out for 10 h in a 5 L fermentation tank and is 6.82 times that of a shake flask, and the purity of F2 reaches up to 82.20% after the reaction is carried out for 24 h finally. The invention not only provides an efficient solution for large-scale production of rare saponin F2, but also provides a transferable framework for designing and engineering diversified glycoside hydrolase in customized biological catalysis.

The invention relates to construction and application of an engineering bacterium for high yield of chitinase ChiD, in particular to a bacillus thuringiensis engineering bacterium deltacdsR (P5014-chiD) strain, and the preservation number of the bacillus thuringiensis engineering bacterium deltacdsR (P5014-chiD) strain is GGMCC No.35124. The HD73 endogenous gene chiD gene is efficiently expressed in the bacillus thuringiensis delta cdsR, and the yield in a fermentation culture medium is far higher than that of a wild strain HD73. Compared with HD73 (P5014-chiD), the compound has a relatively strong inhibition effect on mycelial growth of fusarium graminearum and exserohilum turcicum, and shows a better effect on prevention and treatment of larvae of plutella xylostella compared with the HD73 (P5014-chiD).

The invention discloses a carbon-based biological liquid organic water-soluble fertilizer as well as a preparation method and application thereof, and belongs to the technical field of biological organic fertilizers. The water-soluble fertilizer comprises the following raw materials in parts by weight: 15-25 parts of a seaweed extract, 10-15 parts of potassium fulvate, 5-10 parts of a compound microbial agent, 3-5 parts of potassium dihydrogen phosphate, 2-4 parts of potassium nitrate, 2-3 parts of urea, 2-3 parts of calcium nitrate, 1-2 parts of magnesium sulfate heptahydrate, 1-1.5 parts of a trace element-containing substance, 1-2 parts of sodium dodecyl benzene sulfonate and 0.5-1 part of potassium sorbate. The total amount of the components is 100 parts. According to the invention, three strains such as pseudomonas sevivaldi, bacillus pumila and bacillus thuringiensis are screened to play roles in three dimensions of promoting growth, improving rhizosphere environment and preventing and treating diseases and insect pests respectively, and are combined with organic components such as seaweed extract and potassium fulvate in the fertilizer and other components, so that the fertilizer has a good application prospect. A comprehensive system of nutrition, growth promotion, disease resistance and quality improvement is formed, and the yield and quality of grapes are remarkably improved.

The invention provides a Cry53-like insecticidal protein and application thereof in prevention and treatment of diptera pests, and belongs to the technical field of biopesticides, the Cry53-like insecticidal protein is separated from bacillus thuringiensis, and the amino acid sequence of the Cry53-like insecticidal protein is shown as SEQ ID NO.2. The invention further provides a preparation method of the Cry53-like insecticidal protein. Compared with the reported Cry insecticidal protein, the Cry53-like insecticidal protein has the advantages that the highest homology is only 38%, the Cry53-like insecticidal protein is determined to be a novel insecticidal protein, the Cry53-like insecticidal protein has advantages in prevention and treatment of diptera pests, the killing effect of the Cry53-like insecticidal protein on bradysia odoriphaga is obviously superior to that of other same-type Cry insecticidal proteins, and the Cry53-like insecticidal protein is a novel insecticidal protein. Therefore, the gene has application prospects in the fields of biopesticide creation, transgenic insect-resistant crop cultivation and the like.

The present invention provides a planting method for promoting the early survival and growth of Panax notoginseng, and relates to the technical field of Panax notoginseng planting. The planting method mainly comprises the following steps: disinfecting and hormone-treating the Panax notoginseng seeds in the early stage, fumigating them with a certain concentration of cymene, and then watering them with a sterilized solution of pine needles and corn fermented with Bacillus thuringiensis after sowing. The present invention overcomes the shortcomings of the existing technology, can effectively improve the early emergence efficiency of Panax notoginseng, ensure uniform emergence of seedlings, promote root growth of Panax notoginseng seedlings, increase the vigorous seedling rate of Panax notoginseng seedlings, and comprehensively improve the economic benefits of Panax notoginseng planting.

The invention discloses a hydrogel and probiotic compounded microbial inoculum composition and a vegetable planting matrix, a three-dimensional gel carrier is used for embedding a microbial inoculum, the carrier integrates mechanical properties and thallus protection and can maintain flora activity for a long time, bacillus thuringiensis has a low-oxygen survival characteristic after being subjected to Fnr enhanced expression, and the microbial inoculum composition has the advantages that the microbial inoculum composition has a low-oxygen survival characteristic after being subjected to Fnr enhanced expression; the microbial agent composition can survive for a long time in a low-oxygen environment of a carrier embedding environment, and is cooperated with bacillus cereus, azotobacter chroococcum and bacillus megatherium to promote the growth of plants such as vegetables and the like and directionally kill pathogenic microorganisms such as root-knot nematode. Corresponding pests can be killed in a green and targeted manner, the growth of plants is promoted, and the yield is increased.

The invention discloses an insecticidal composition containing fluchlorantraniliprole and a biogenic insecticide, which comprises an active component A and an active component B. The active component A is selected from fluchlorantraniliprole, the active component B is selected from fluchlorantraniliprole, and the active component C is selected from fluchlorantraniliprole. The active ingredient B is selected from any one of abamectin, spinosad, spinetoram, bacillus thuringiensis, milbemectin, methylamino abamectin, methylamino abamectin benzoate (emamectin benzoate), sucrose octacarboxylate, beauveria bassiana, metarhizium anisopliae, nuclear polyhedrosis virus, cytoplasmic polyhedrosis virus and granulosis virus; the weight ratio of the active component A to the active component B is (1: 80)-(80: 1). The composition disclosed by the invention has a synergistic effect on various pests harmful to agricultural production, reduces the dosage of pesticides, reduces the residual quantity of the pesticides on crops, relieves environmental pollution, is safe to human and livestock, has good environmental compatibility, and delays the generation of pest resistance.

The invention relates to construction and application of RNase III deletion type bacillus thuringiensis (RNase III). The bacterial strain is obtained by taking a bacillus thuringiensis engineering bacterial strain DB delta 189 as an initial bacterial strain and knocking out an rnc gene by adopting a gene editing method. The strain realizes oral delivery of dsRNA and effective silencing of a target gene; meanwhile, the insecticidal effect of the Bt strain HD1 can be obviously enhanced. According to the invention, a new action mechanism is introduced while the traditional insecticidal advantages of Bt are reserved, so that the insecticidal effect of the Bt strain is improved, and the application prospect of Bt in comprehensive pest control is expanded.

This invention belongs to the field of biodegradation promoter technology, specifically relating to a green synthesis method for a biodegradation promoter of cyclic olefin polymers. The invention involves aerobic fermentation of a mixture of *Bacillus thuringiensis*, *Bacillus licheniformis*, and *Candida*. The fermented product contains abundant enzymes and bioactive agents, which are beneficial for promoting the degradation of cyclic olefin polymers. Furthermore, by using modified chitosan to immobilize the fermented product, microorganisms and their metabolites can be fixed on the chitosan surface, promoting the full release of active functions. In addition, the stability of the modified chitosan structure is improved through a cross-linking reaction. Therefore, the biodegradation promoter provided by this invention can not only promote the biodegradation of cyclic olefin polymers but also be used in the preparation of biodegradable products containing cyclic olefin polymers.

The invention relates to a single-chain antibody for recognizing Vip3Aa toxin of bacillus thuringiensis and application of the single-chain antibody. The single-chain antibody is constructed by taking a mouse hybridoma cell strain secreting a monoclonal antibody for resisting the Vip3Aa toxin as a template; the nucleotide sequence and the amino acid sequence are respectively shown as SEQ ID NO. 1 and SEQ ID NO. 2; the single-chain antibody can be used as a detection antibody, is applied to detection of Vip3Aa toxin by a double-sandwich ELISA (enzyme-linked immuno sorbent assay) method, is high in sensitivity and specificity, and can be used for rapid large-scale screening of Vip3Aa toxin in transgenic crops.

The invention belongs to the technical field of insect pest control, and particularly discloses a complex microbial inoculant for preventing and treating insect pests and application thereof.The complex microbial inoculant is prepared from, by weight, active ingredients and auxiliary materials, the active ingredients comprise 5-8 parts of bacillus thuringiensis, 4-6 parts of beauveria bassiana, 3-5 parts of metarhizium anisopliae, 2-4 parts of bacillus subtilis, 2-3 parts of bacillus amyloliquefaciens and 1-3 parts of paecilomyces lilacinus, the auxiliary materials comprise the following components in parts by weight: 1-2 parts of a surfactant, 0.5-1 part of a protective agent, 2-3 parts of a nutrient synergist and 30-40 parts of a carrier. According to the complex microbial inoculant, multiple functional microorganisms and synergistic components are reasonably matched, comprehensive and efficient prevention and control of scale insects, red spiders, aphids and fruit borers are achieved, and the complex microbial inoculant is environmentally friendly and safe to durian growth. The defects that durian pest control depends on chemical pesticides, and the control effect of microbial agents is single and unstable in the prior art are overcome.

The invention relates to bacillus thuringiensis KHWY-1 with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No.33400. The strain is used for fermenting feathers for 20 hours under the conditions that the temperature is 25 DEG C and the pH value is 8, the soluble protein content reaches 691.37 mu g / mL, and the feather degradation rate reaches 90% or above. The invention also relates to a new application of the bacillus thuringiensis KHWY-1 in degrading the feather fermentation filtrate of the white feather chicken as a cosmetic raw material, and the additional value of feather degradation products is improved.

The application discloses a saline-alkali soil special rice bio-organic fertilizer, which comprises halomonas, bacillus subtilis, paenibacillus polymyxa, bacillus thuringiensis, lactobacillus plantarum, longovis rubescens and organic fertilizer. In actual preparation, the microorganism is expanded to obtain a microbial agent, then the rotten cow dung and auxiliary materials are uniformly mixed, granulation drying is carried out, and after cooling treatment, the microbial agent and a coating agent mixed solution are added to carry out coating treatment, and then packaging is carried out. After the finished product is packaged, it can be used after being stored in a warehouse for a period of time. The microbial fertilizer has high organic matter content and active bacteria quantity, and in combination with conventional irrigation and fertilization measures, can improve the soil structure of saline-alkali soil, reduce the soil salt content and pH, enhance the stress resistance of crops, promote the growth of rice, and increase the yield, so that the effects of improving soil, fertilizing, resisting stress and promoting growth are achieved.

The invention discloses a compound microbial agent rich in beta-glucan as well as a preparation method and application of the compound microbial agent, and belongs to the technical field of agricultural biology. According to the microbial agent, an aureobasidium pullulans-rhizobium pullulans-bacillus subtilis-bacillus thuringiensis quaternary synergistic system is constructed, specific auxiliary materials, carriers and a coating technology are supplemented, and under the drought stress condition, the microbial agent can remarkably promote the growth (plant height and biomass) of the pakchoi, so that the yield of the pakchoi is increased, and the yield of the pakchoi is increased. The yield of the pakchoi is increased, the quality is improved (the content of soluble sugar and protein is increased), the chlorophyll content and the photosynthetic efficiency are improved, the antioxidant enzyme activity is enhanced, cell membrane damage is reduced, the physical and chemical properties of soil are improved, and a new effective solution is provided for agricultural green drought resistance.

The invention relates to a strain BtJC2602 with high activity on phyllotreta striolata and application of the strain BtJC2602. The bacillus thuringiensis wild strain BtJC2602 is screened out from the environment, and the preservation number of the bacillus thuringiensis wild strain BtJC2602 is CGMCC (China General Microbiological Culture Collection Center) No.33742. The strain BtJC2602 provided by the invention has activity on phyllotreta striolata larvae, and realizes larva control on phyllotreta striolata. The activity of the strain BtJC2602 provided by the invention on larvae is higher than that of the existing strain G033A. The bacterial strain provided by the invention also has very high killing activity on similarly hetidaje.

The invention relates to a strain BtJC2604 with high activity on phyllotreta striolata and application of the strain BtJC2604. The bacillus thuringiensis wild strain BtJC2604 is screened from the environment by the applicant, and the preservation number of the bacillus thuringiensis wild strain BtJC2604 is CGMCC (China General Microbiological Culture Collection Center) No.36685 The strain BtJC2604 provided by the invention has activity on phyllotreta striolata larvae, and realizes larva control on phyllotreta striolata. The activity of the strain BtJC2604 provided by the invention on larvae is higher than that of the existing strain G033A.

The invention belongs to the field of microbial agents and plant growth promotion, and particularly relates to bacillus altitudinis YN18 and application thereof in corn growth promotion. The preservation number of the bacillus altitudinis YN18 is CCTCC (China Center for Type Culture Collection) NO: M 20251694, and the bacillus altitudinis YN18 is preserved in the China Center for Type Culture Collection on July 24, 2025; the invention relates to a bacillus altitudinis YN18, which is classified and named as bacillus altitudinis YN18. According to the invention, a strain of bacillus altitudinis YN18 is obtained through separation for the first time, and the strain YN18 has good siderophore production and IAA secretion capabilities, and can significantly promote corn plant height, root length, fresh root weight and dry root weight; therefore, the method has a good application prospect. The bacillus altitudinis YN18 provided by the invention has a remarkable emission reduction effect on NO emission of corn plants, the actually measured emission reduction rate is up to 106.52%, and the bacillus altitudinis YN18 shows excellent greenhouse gas regulation potential.

This invention relates to a class of compounds containing isopropanolamine substructures, their preparation methods, and applications. These compounds have the structure shown in general formula (I). Based on 4-chlorothiophenol, this invention introduces a nitrogen-containing fragment into this system to synthesize a series of compounds containing isopropanolamine substructures. These compounds exhibit excellent inhibitory effects on plant pathogenic bacteria such as *Bacillus thuringiensis*, *Citrus canker*, *Rhizoctonia solani*, and *Ophiopogon japonicus*.

The application discloses a preparation method of an extractive for preventing and treating citrus anthracnose, and comprises the following steps: drying garlic, astragalus, ligusticum wallichii, banana peel, soybean, silkworm chrysalis and plantago seed, and then crushing the dried garlic, astragalus, ligusticum wallichii, banana peel, soybean, silkworm chrysalis and plantago seed; adding the crushed garlic, astragalus, ligusticum wallichii, banana peel, soybean, silkworm chrysalis and plantago seed into boiling water and stirring until uniform; continuing to cook; freezing; thawing after the freezing is completed; obtaining a freeze-thawed material; adding sodium dihydrogen phosphate into the freeze-thawed material; placing the freeze-thawed material in a fermentation tank; inoculating bacillus subtilis and bacillus mycoides for aerobic fermentation; inoculating bacillus licheniformis, bacillus laterosporus and bacillus thuringiensis for aerobic fermentation; completing the aerobic fermentation; carrying out anaerobic fermentation on the fermented material; stirring the fermented material and pure water until uniform; filtering after standing; taking filter residue and precipitate as extractive A; adjusting the PH value of filtrate to obtain extractive B; adding bagasse powder into the extractive A, compacting, packaging and bagging; and sealing and storing the extractive B. The extractive prepared by the application can efficiently prevent and treat citrus anthracnose, and has the characteristics of low production cost and no pollution to citrus planting environment.

The invention belongs to the technical field of microorganisms, and discloses bacillus thuringiensis BMB4196 with high yield of thurinins and application of the bacillus thuringiensis BMB4196. The preservation number of the strain is CCTCC M 2026098, the strain has a remarkable high-yield characteristic of the thuringiensis, and the yield of the thuringiensis can reach 22.52 g / L under the condition of a 50L fermentation tank, which is the highest yield level of the thuringiensis reported at present. The strain is suitable for large-scale fermentation production of the thuringiensis, can effectively reduce the production cost and improve the production efficiency, provides an important strain resource for industrial application and product development of the thuringiensis, and has a good application prospect.

The present application relates to the technical field of antibacterial material, in particular to a microencapsulated nanometer silver composite material with long-acting antibacterial function and a preparation method thereof.The preparation method of the microencapsulated nanometer silver composite material with long-acting antibacterial function comprises the following steps: fermenting culture medium by using a composite microorganism composed of Bacillus thuringiensis and Leucothrix sp., obtaining a fermentation liquor, and then carrying out microwave reaction on the fermentation liquor and a silver nitrate solution containing an emulsifier and a dispersing agent to obtain a nanometer silver solution, and then reacting the nanometer silver solution with a quaternary ammonium chitosan solution to obtain the microencapsulated nanometer silver composite material.The nanometer silver obtained by using the preparation method has a particle size of only 5.7-9.1nm, and the stability of the nanometer silver can be improved through microencapsulation, so that the silver ions are released slowly, the antibacterial time is prolonged, and a high-efficiency antibacterial effect is achieved.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera and Coleoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.