57 results about "Wild strain" patented technology

The invention discloses kluyveromyces marxianus and application of the kluyveromyces marxianus in production of single-cell protein by taking straw as a raw material. The Kluyveromyces marxianus is a ZH-2 bacterial strain which is CGMCC (China General Microbiological Culture Collection Center) No.32295 The strain is obtained from a wild strain in a laboratory adaptive evolution mode, wheat straw and an inorganic nitrogen source can be used as raw materials for efficiently producing single-cell protein, compared with a parent strain, the utilization speed and the utilization rate of biomass, glucose and xylose are remarkably increased, the yield of ZH-2 SCP can reach 0.047 g.g <-1 >, and the yield of ZH-2 SCP can reach 0.045 g.g <-1 >. And the essential amino acid mode basically accords with adult and infant amino acid modes, and can be used as high-quality protein nutrition supply. The Kluyveromyces marxianus ZH-2 strain capable of obtaining single-cell protein by fermenting wheat straw as a raw material is provided from the perspective of utilizing cheap waste resources, so that the production cost of SCP is greatly reduced, and the problem of protein resource shortage is effectively solved.

The invention provides a nucleic acid probe, a primer group, a kit, a detection method and application, and relates to the technical field of biology. The nucleotide sequence of the nucleic acid probe is as shown in SEQ ID NO. 1; wherein the 3'end of the nucleic acid probe is modified with MGB, and locked nucleic acid is modified at a base position corresponding to the difference between the swine fever wild strain and the swine fever vaccine strain in the nucleotide sequence. According to the nucleic acid probe, a specific sequence is utilized to precisely target a swine fever wild strain, and locked nucleic acid modification is introduced at a differential site, so that the recognition capability on single-base mismatch is remarkably enhanced, non-specific binding to a vaccine strain is thoroughly blocked, and false positive interference is eliminated; and 3 '-terminal MGB modification is matched to improve the binding stability and sensitivity, so that the efficient and accurate identification of the swine fever wild strain under the immune background is ensured.

The invention discloses recombinant xanthomonas campestris with xanthan gum hydrolase displayed on the surface and a construction method and application of the recombinant xanthomonas campestris. The recombinant xanthomonas campestris for surface display of xanthan hydrolase is constructed by introducing a recombinant vector obtained by fusion expression of an ice crystal nuclein (INP) gene and a xanthan hydrolase gene into xanthomonas campestris. The gel yield of recombinant xanthomonas campestris fermentation is twice that of a wild strain and reaches 6.7 g / L. The strain can efficiently hydrolyze xanthan gum with high molecular weight (such as more than 100 wDa), the traditional production process which is relatively complicated is simplified, and a new method is provided for large-scale production of the xanthan gum with low molecular weight.

The application belongs to the technical field of biotechnology, and particularly relates to a human metapneumovirus mouse-adapted strain and application thereof. Compared with wild strains, the strain can exhibit stronger susceptibility to animals, and can cause typical clinical symptoms and even death. For example, after the strain infects BALB / c mice, the disease symptoms are obvious, 83% of the mice have a severe decrease in body weight to below 75% after infection, and have reached the humane killing standard. Four days after the strain infects BALB / c mice, the mortality rate is above 50%. The strain can be used to construct a cell model or an animal model of human metapneumovirus infection or a disease caused by the human metapneumovirus, and is further used to screen drugs for preventing and / or treating human metapneumovirus infection or a disease caused by the human metapneumovirus. In addition, the strain can also be used to prepare drugs for preventing and / or treating human metapneumovirus infection or a disease caused by the human metapneumovirus, and to prepare antibodies or antisera against the human metapneumovirus.

The invention relates to the technical field of biological medicines, and discloses a construction method and expression application of an Hcp gene deleted strain of a rahnella aqua KCL-5 strain for fish. The Hcp 0186 gene deletion strain is constructed by using a homologous recombination method, the comparison between the deletion strain and a wild strain is carried out, and the result shows that the deletion of the Hcp 0186 gene has an influence on the growth speed, the pathogenicity of the Hcp 0186 gene to the zebra fish is obviously reduced, the survival rate of the infected zebra fish is increased from 0% to 52%, and the Hcp 0186 gene deletion strain can be used as a fish attenuated live vaccine. A recombinant prokaryotic expression vector pET32a-Hcp and expression and purification of fusion protein of the recombinant prokaryotic expression vector pET32a-Hcp are further constructed, and Hcp 0186 rabbit polyclonal antibody serum is prepared as a primary antibody and can be applied to localization expression analysis of fish tissues infected by the bacterium and detection of WB and IHC. Results prove that the Hcp 0186 gene deletion of the strain provides an attenuated strain vaccine and clinical diagnosis and detection application of infected fish of the attenuated strain vaccine, and a scientific basis can be provided for revealing a molecular pathogenic mechanism of the strain and development and application of a mutant strain of the strain.

The invention relates to the technical field of virus detection, in particular to a fluorescent RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection probe 1106VP and / or 1106WP and primers 1106VWF and 1106VWR. The invention also discloses a fluorescent RT-PCR detection reagent, a fluorescent RT-PCR detection kit, a fluorescent RT-PCR detection probe, a primer, the fluorescent RT-PCR detection reagent and the application of the fluorescent RT-PCR detection kit in identification of a porcine reproductive and respiratory syndrome NJ-1106R strain and a porcine reproductive and respiratory syndrome non-NJ-1106R strain. The probe and the primer disclosed by the invention are high in specificity and high in sensitivity; the accuracy is high, and the accuracy rate of blind sample detection is 100%; the kit is high in universality, can be used for detecting virus liquid for cell culture in a laboratory, and can also be used for detecting porcine serum and tissue samples in clinical samples. The method is an effective method for identifying the PRRSV NJ-1106R vaccine strain and other PRRSV wild strains.

The invention relates to construction and application of an engineering bacterium for high yield of chitinase ChiD, in particular to a bacillus thuringiensis engineering bacterium deltacdsR (P5014-chiD) strain, and the preservation number of the bacillus thuringiensis engineering bacterium deltacdsR (P5014-chiD) strain is GGMCC No.35124. The HD73 endogenous gene chiD gene is efficiently expressed in the bacillus thuringiensis delta cdsR, and the yield in a fermentation culture medium is far higher than that of a wild strain HD73. Compared with HD73 (P5014-chiD), the compound has a relatively strong inhibition effect on mycelial growth of fusarium graminearum and exserohilum turcicum, and shows a better effect on prevention and treatment of larvae of plutella xylostella compared with the HD73 (P5014-chiD).

The application discloses a portable gradient freeze-drying preservation device for wild strain germplasm resources, and relates to the technical field of wild fungus preservation.The application comprises a preservation box, a box cover is rotationally matched with the preservation box, a trapezoidal wild fungus placing rack is slidably matched in the preservation box, and a plurality of universal wheels are arranged at the bottom of the preservation box;an elevating assembly connected with the trapezoidal wild fungus placing rack is arranged on one side in the preservation box, the elevating assembly is matched with the box cover, and an intelligent preservation system is arranged in the preservation box.A temperature gradient control unit is arranged in the application, and a precise controllable temperature gradient can be formed in different level areas of the trapezoidal wild fungus placing rack according to the optimal preservation temperature requirements of different wild fungi.For example, the upper area of the placing rack can be set as a relatively high temperature interval to adapt to some wild fungi which prefer warm environment.

The application belongs to the field of bioengineering and particularly relates to a Bradyrhizobium elkanii with improved nodulation capacity and a construction method thereof. The pip gene of the Bradyrhizobium elkanii FG-486 is knocked out to improve the nodulation capacity of the Bradyrhizobium elkanii FG-486, and the Bradyrhizobium elkanii FG-486 delta P with improved nodulation capacity is obtained. Compared with the wild strain FG-486, the average nodulation number of soybean plants after seed dressing with the Bradyrhizobium elkanii FG-486 delta P is increased by 31.15%, the root nodule dry weight is increased by 10.26%, and the soybean yield is increased by 7.85%.

The invention discloses a pharmaceutical composition for preventing and treating novel coronavirus infection, the pharmaceutical composition comprises a phthalate compound, and the phthalate compound has a structure as shown in formula I (formula I); in the formula I, R1 and R2 are the same or different and are independently selected from substituted or unsubstituted straight chain or branched chain alkyl with the carbon atom number of 1-20. The pharmaceutical composition takes a phthalate compound as an active ingredient, cell membrane fusion mediated by combination of S protein of the novel coronavirus SARS-CoV-2 and a target cell receptor ACE2 is blocked, the inhibition effect on SARS-CoV-2 virus infection is achieved, in addition, the phthalate compound not only has a remarkable inhibition effect on SARS-CoV-2 wild strains, but also has a remarkable inhibition effect on the SARS-CoV-2 wild strains. And the SARS-CoV-2 mutant strain also shows a broad-spectrum antiviral effect, and is suitable for intervention of early, mild and asymptomatic novel coronavirus infection.

The invention belongs to the field of improvement of acid tolerance and high-density fermentation of probiotics, and particularly relates to a domestication method for improving acid tolerance and fermentation density of lactobacillus plantarum and an obtained strain. The invention provides a domestication method for improving acid tolerance and fermentation density of lactobacillus plantarum, which is characterized in that Lactiplantarum ZJ316 (CCTCC NO: M 208077) is used as a wild strain, the wild strain is domesticated, and the obtained domesticated strain ZJ316PV (Lactiplantarum ZJ316PV) has the preservation number of CGMCC NO.33082. The acclimatization method for improving acid tolerance and fermentation density of lactobacillus plantarum has the advantages that the acclimatization method comprises the following steps: taking Lactiplantarum ZJ316 (CCTCC NO: M 208077) as the wild strain; the acid tolerance of the domesticated strain ZJ316PV is superior to that of the wild strain ZJ316. The invention also provides a method for improving the PV fermentation density of the wild strain ZJ316 and the domesticated strain ZJ316.

The invention belongs to the technical field of gene engineering, and particularly relates to aureobasidium pullulans with high yield of pullulan and application of the aureobasidium pullulans. On the basis of aureobasidium pullulans M233-20, an isopullulanase gene A9187 gene in a genome of the aureobasidium pullulans M233-20 is knocked out based on a homologous recombination technology, and through polysaccharide fermentation test detection, an isopullulanase gene deletion mutant strain aureobasidium pullulans delta A9187-1 with the pullulan polysaccharide molecular weight improved and the yield not reduced is obtained. The weight-average molecular weight of the pullulan produced by the recombinant strain is 6.5 * 10 < 5 > Da, which is 2.6 times that of the original wild strain (2.46 * 10 < 5 >), and the yield of the pullulan is increased from 147.33 g / L to 156 g / L, which is increased by 5.88%.

The invention belongs to the technical field of genetic engineering, and particularly relates to hidden plasmid mediated genetic engineering probiotics as well as a construction method and application thereof. According to the method, stable genetic expression of exogenous genes is mediated through high-copy hidden plasmids, resistance does not need to be added in the culture process, and the safety, stability and effectiveness of synthesis of food-grade nutritional chemicals are remarkably improved. The modified strain can efficiently utilize glucose as a carbon source to synthesize a functional nutritional chemical VB5 product, the titer of VB5 in shake flask fermentation reaches 102 mg / L, and the product is not detected by a wild strain. The invention provides a new strain and technical support for food-grade efficient synthesis of functional nutritional chemicals, and is beneficial to popularization and application in the field of food.

The present invention belongs to the technical field of biopharmaceuticals and specifically relates to use of a pharmaceutical composition in combating mycobacterial infection. The use of zuclopenthixol in combination with bedaquiline exhibits significantly enhanced anti-mycobacterial infection activity. Compared to the use of bedaquiline alone, the use of 2 μg / ml zuclopenthixol in combination with bedaquiline results in varying reductions in colony counts of the MmpL5-MmpS5 strain when the concentration of bedaquiline ranges from 1 / 16 MIC to 2 MIC. As an enhancer of bedaquiline, zuclopenthixol can enhance the anti-mycobacterial infection activity of bedaquiline. The minimum inhibitory concentration of zuclopenthixol against the Rv0678 mutant strain in vitro is 16 μg / ml, and the minimum inhibitory concentration of BDQ against the Rv0678 mutant strain in vitro is 1 μg / mL. The use of 1 μg / ml zuclopenthixol in combination with BDQ reduces the MIC of BDQ against the Rv0678 mutant strain from 1 μg / ml to 0.03 μg / ml (a 32-fold increase in efficacy), which is lower than the MIC against the wild strain H37Rv (0.06 μg / ml), reversing resistance to BDQ and further enhancing the anti-mycobacterial infection activity.

The invention relates to a method for increasing the sporulation of bacteria such as Bacillus velezensis and Paenibacillus polymyxa using a liquid medium comprising glucose, ammonium sulfate, magnesium chloride and non-ionic surfactant polymer compounds. This method for increasing sporulation is used in a method for generating a large quantity of spores and selecting spores more resistant to higher temperatures than the spores of wild strains. The spores with higher heat resistance than those of the wild strains can be used in a method for eliminating contamination in fermentations.

The application discloses an ELISA antibody detection kit for identifying Brucella gene deletion live vaccine (M5-90△26 strain) and wild strains and application. The kit comprises enzyme-linked reaction plate 1 and enzyme-linked reaction plate 2. The enzyme-linked reaction plate 1 is coated with LPS extracted from the Brucella gene deletion live vaccine (M5-90△26 strain), and the enzyme-linked reaction plate 2 is coated with a Brucella BP26 antigen epitope polypeptide combination composed of polypeptides with amino acid sequences shown in SEQ ID NO:1-2. The application is suitable for goats and sheep inoculated with the Brucella gene deletion live vaccine (M5-90△26 strain), can effectively distinguish the antibody produced by the Brucella gene deletion live vaccine (M5-90△26 strain) and the antibody produced by wild strain infection, thereby realizing identification and detection, being favorable for prevention and control of brucellosis and optimization of a vaccination strategy, and providing technical support for healthy and sustainable development of animal husbandry.

The invention provides a bacillus thuringiensis engineering strain for efficiently preventing and treating plutella xylostella, the engineering strain carries a nucleic acid fragment for preparing dsRNA, and the prepared dsRNA can interfere with the expression of a plutella xylostella mitogen activated protein kinase (MAP4K4) gene; the bacillus thuringiensis engineering strain is a Bt strain B-T-3 of which the preservation number is CCTCC (China Center for Type Culture Collection) No: M20252327. The engineering bacterium provided by the invention has a strong silencing effect on plutella xylostella MAP4K4 gene expression and shows high insecticidal activity on plutella xylostella larvae, the median lethal concentration on the plutella xylostella larvae is 2.3 * 10 < 6 > cfu / mL, and the activity is 3.2 times that of a wild strain. The result shows that the dsRNA and the Bt strain have synergistic toxicity.

The application discloses a high-temperature inducible promoter and application thereof, and belongs to the field of genetic engineering and fermentation engineering. Acetobacter pasteurianus A high-temperature inducible promoter P icd is screened from a bacillus pabuli (Olsenella ) and has a nucleotide sequence as shown in SEQ ID NO. 1. The application further constructs a recombinant expression vector pBBR1MCS2-icd containing the promoter and a recombinant bacterium expressing an ethanol dehydrogenase gene AdhP. The recombinant strain ApAdhP(icd) using the promoter to regulate the expression of the ethanol dehydrogenase can not only reduce the metabolic burden of the cell and maintain a good growth trend under high-temperature fermentation conditions, but also can reach an acetic acid final yield of 52.65 g / L, which is increased by about 31.5% compared with a wild strain. The application effectively solves the problems of the decrease of acetic acid production rate and yield under high-temperature environment in summer in traditional acetic acid fermentation.

The invention discloses a gene FgPIP1 for regulating and controlling polar growth and pathogenicity of the top end of fusarium graminearum and application of the gene FgPIP1, and belongs to the field of plant disease prevention and control. According to the invention, a novel pathogenic gene FgPIP1 with specific existence of filamentous ascomycetes is found in plant pathogenic fungi fusarium graminearum, and the nucleotide sequence of the pathogenic gene FgPIP1 is shown as SEQ ID NO. 1. Compared with a wild strain PH-1, the FgPIP1 gene knockout mutant has the advantages that the diameter of a vegetative growth colony of the mutant is reduced, the number of apical branches of hyphae is increased, and the pathogenicity to wheat ears is reduced, which indicates that the FgPIP1 gene plays an important role in regulating and controlling the pathogenic process of fusarium graminearum. The invention provides a molecular basis for developing a novel bactericide by utilizing the FgPIP1 gene and taking inhibition of the top growth of filamentous fungus hyphae as a target spot.

The invention provides a breeding application of a cotton GhD05G2577 gene in increasing the plant germination rate, the biomass, the seed size and the thousand seed weight. Through a transgenic technology, an arabidopsis thaliana homozygous line plant over-expressing the GhD05G2577 gene is obtained. Compared with a wild strain, the early germination rate of a transgenic plant in a 1 / 2 MS plate is obviously increased; in the seedling stage, the leaf number is increased, the leaf length and the leaf width are increased, the plant height is increased, the chlorophyll content SPAD is increased, and the fresh weight, the dry matter content and the biomass accumulation are remarkably increased; the fruit shows that the legume length and width are increased, the seed length and width are increased, and the thousand seed weight is increased, and all reach a significant level. When the gene is applied to high-yield and income-increasing breeding of plants, the germination rate of the plants can be increased, biomass accumulation of the plants can be increased, the seed size and thousand seed weight can be increased, and an alternative scheme is effectively provided for high-yield molecular breeding of the plants.

The invention relates to a strain BtJC2604 with high activity on phyllotreta striolata and application of the strain BtJC2604. The bacillus thuringiensis wild strain BtJC2604 is screened from the environment by the applicant, and the preservation number of the bacillus thuringiensis wild strain BtJC2604 is CGMCC (China General Microbiological Culture Collection Center) No.36685 The strain BtJC2604 provided by the invention has activity on phyllotreta striolata larvae, and realizes larva control on phyllotreta striolata. The activity of the strain BtJC2604 provided by the invention on larvae is higher than that of the existing strain G033A.

The present application relates to a kind of monoclonal antibody DIVA55 for swine fever virus in the field of medical preparation and its preparation method and application.The monoclonal antibody DIVA55 or its antigen-binding fragment of the present application includes heavy chain variable region and light chain variable region;The amino acid sequences of LCDR1, LCDR2 and LCDR3 in the light chain variable region of the antibody are as shown in the 24th to 34th, 50th to 56th, 89th to 97th of SEQ ID No:4;The amino acid sequences of HCDR1, HCDR2 and HCDR3 in the heavy chain variable region of the antibody are as shown in the 31st to 35th, 50th to 65th, 95th to 102nd of SEQ ID No:5.The monoclonal antibody of the present application can be used in the field such as swine fever E2 protein marker subunit vaccine immunization and wild strain serological diagnosis, swine fever vaccine immunization effect evaluation and related experiments, provides antibody resource for CSF prevention and control and purification.

The application belongs to the technical field of biology, and particularly relates to a 3D protein mutant O foot-and-mouth disease recombinant virus strain and construction and application thereof. The application mutates methionine at the 403th position of the 3D protein of the O foot-and-mouth disease virus into alanine, and successfully constructs and rescues a 3D protein mutant O foot-and-mouth disease recombinant virus strain rFMDV-M403A by using reverse genetic technology. The amino acid of the recombinant virus strain can be stably mutated in the process of subculture, and the growth speed of the mutant strain is slower than that of the wild strain, and the virus titer is significantly reduced. In individual experiments, the infection of the mutant strain to suckling mice shows that the mutant strain can improve the survival rate of the suckling mice, and significantly reduce the viral load in the liver and the tissue lesions. The application provides an attenuated foot-and-mouth disease recombinant virus strain, which has important significance for in-depth understanding of the pathogenic mechanism and prevention and control strategy of the foot-and-mouth disease virus, and provides an important theoretical basis for further development of the foot-and-mouth disease genetic engineering vaccine.

The invention belongs to the technical field of microorganisms, and particularly relates to recombinant lactobacillus reuteri with helicobacter pylori resistance as well as construction and application of the recombinant lactobacillus reuteri. The recombinant lactobacillus reuteri is engineered to express a gram-negative bacillus outer membrane penetrating peptide and a helicobacter pylori bacteriophage HP30 splitting system protein Artilysin 2. The recombinant lactobacillus reuteri can inhibit the growth of helicobacter pylori and relieve GES-1 cell inflammation and mouse gastric mucosa inflammation induced by helicobacter pylori ATCC43504, and compared with a wild strain, the recombinant lactobacillus reuteri remarkably improves the helicobacter pylori resisting effect. In addition, the recombinant lactobacillus reuteri can tolerate gastric acid of a mouse and is colonized in gastric mucosa of the mouse, the purpose of remarkably resisting helicobacter pylori by single probiotics is achieved, meanwhile, a new treatment thought is provided for treatment of helicobacter pylori, and a new probiotic therapy is created for treatment of helicobacter pylori.

The invention discloses a rotavirus reassortment attenuated strain LG12 and application of the rotavirus reassortment attenuated strain LG12. The rotavirus reassortment attenuated strain LG12 comprises: i) a coding gene of a structural protein VP7 of a human G12 type rotavirus strain G12P [6]; and ii) coding genes of structural proteins VP1, VP2, VP3, VP4 and VP6 and non-structural proteins NSP1, NSP2, NSP3, NSP4 and NSP5 of the sheep rotavirus strain LLR. The reassortment strain provided by the invention has the safety of animal strains and the immunogenicity of human G12 type RV, can effectively prevent infection of human G12 type wild strains, and can have different degrees of cross protection on infection of other various G type RV wild strains; the method has great significance for developing RV vaccines which cover more G and P genotypes of Chinese epidemic RVA, have stronger prevention pertinence and provide a better protection effect on Chinese infantile diarrhea.

This invention relates to an antibiotic-label-free, induced, salt-tolerant Rhodotorula globulinii engineered strain and its applications, belonging to the field of microbial technology. Wild Rhodotorula globulinii is a dominant species for wastewater biological treatment, but natural strains grow extremely slowly at salt concentrations exceeding 3%, significantly inhibiting their ability to degrade pollutants. The induced, salt-tolerant Rhodotorula globulinii engineered strain constructed in this invention, after lactose induction, significantly improves the treatment capacity of ammonia nitrogen, phosphate, and acetate in 3%–5% high-salt wastewater compared to wild strains and constitutive overexpression engineered strains. This provides technical support for the application of Rhodotorula globulinii in coastal industrial wastewater purification, saline-alkali land ecological restoration, and seawater recycling, significantly enhancing the competitiveness of Rhodotorula globulinii in low-cost seawater cultivation, high-salt wastewater treatment, and biomanufacturing in special environments.

The invention relates to the field of research and development of veterinary biological products, in particular to a porcine rotavirus LNSY5 strain and application thereof. The porcine rotavirus strain LNSY5 provided by the invention is an epidemic porcine rotavirus wild strain, and the vaccine composition containing the porcine rotavirus strain can enable an animal body to quickly generate an antibody after immunizing an animal, and has good prevention and control effects on current epidemic porcine rotavirus infection.

The invention discloses a propionibacterium mutant strain with high propionic acid tolerance as well as a mutation breeding method and application of the propionibacterium mutant strain, and belongs to the technical field of industrial microorganism breeding. The mutant strain is named as propionibacterium CCTCC M20252200D5-6, the propionic acid tolerance upper limit of the mutant strain reaches 85 g / L, and the propionic acid yield of the mutant strain in a 50 L fermentation tank is 76 g / L and is increased by 25.25% compared with that of a wild strain. The breeding method comprises the following steps: 1) carrying out ARTP mutagenesis (treatment is carried out for 80s, and the fatality rate is 92.2%); 2) adaptive evolution of gradient propionic acid pressure (30-80g / L); (3) carrying out primary screening on a 100g / L propionic acid flat plate; 4) carrying out targeted screening on bacterial colonies with high content of trehalose by virtue of a Raman spectrum (532nm excitation, 850cm <-1 > characteristic peak); 5) fermentation verification; the method solves the problems of low screening efficiency and poor positive mutation rate of traditional breeding, the screening flux reaches 1000 strains per day, the period is shortened to 5 days, and the method can be directly applied to industrial production of propionic acid through a microbial fermentation method and has remarkable economic and environmental benefits.

The present application provides a target point, a kit and a method for identifying Brucella vaccine strains and wild strains. The present application obtains a specific nucleotide sequence capable of specifically distinguishing Brucella vaccine strains and wild strains through a large number of research and analysis, which can be used as an effective target point for identifying Brucella vaccine strains and wild strains. The present application also provides a primer composition for multiplex RPA amplification of the target point, and a crRNA composition for detecting the specific nucleotide sequence based on the CRISPR / Cas12a technology. Using the crRNA composition for CRISPR / Cas12a detection of the product of the multiplex RPA amplification, the multiplex amplification and typing detection can be quickly and efficiently (about 35 min) completed, and the minimum detection limit is 5 copies / μL, which has good specificity and sensitivity.

The invention discloses a biosynthetic gene cluster of polyether polydecalinmycin and an application of the biosynthetic gene cluster in construction of a high-yield strain. According to the invention, a biosynthetic gene cluster pdm of the polyether polydecalinmycin is positioned in a genome of streptomyces marincalensis SCSIO 03032, and a high-yield strain of the polydecalinmycin is successfully constructed by overexpressing a positive regulation gene pdmRI or pdmRII coded by the pdm gene cluster in a wild strain, so that the yield of the polydecalinmycin is increased by 48 times, and a material basis is provided for large-scale preparation, development and application of the polydecalinmycin.