103 results about "Wild strain" patented technology

The invention relates to the technical field of carotenoid cleavage dioxygenase, and discloses a carotenoid cleavage dioxygenase mutant and application thereof. In order to improve the efficiency of converting carotenoid in tobacco into aroma molecule beta-ionone, specific mutation of characteristic sites is carried out on carotenoid cleavage dioxygenase (PhCCD1) from petunia, that is, serine (S) at the 428 site of the carotenoid cleavage dioxygenase is mutated into cysteine (C) or tyrosine (Y) to obtain a mutant S428C or S428Y, and the mutant S428C or S428Y is converted into beta-ionone. The yield of the beta-ionone converted from the carotenoid is greatly improved. Experiments prove that the yields of beta-ionone of S428C and S428Y respectively reach 54.2 mg / L and 50.6 mg / L, which are respectively increased by 69.7% and 58.5% compared with those of a wild strain WT.

The invention belongs to the technical field of microorganisms, and particularly relates to stenotrophomonas maltophilia yg3 and application thereof. The invention discloses stenotrophomonas maltophilia yg3, and the preservation number of the stenotrophomonas maltophilia yg3 is CGMCC (China General Microbiological Culture Collection Center) No.34131. The stenotrophomonas maltophilia yg3 disclosed by the invention has the capability of efficiently synthesizing indoleacetic acid (IAA), and the IAA yield of 98.10 mg / L can be realized without optimization in an NB culture medium containing 5mM L-tryptophan, which is obviously higher than that of other wild strains. Moreover, the stenotrophomonas maltophilia yg3 has a remarkable growth promoting effect on plants (such as hot peppers), pot experiments show that the stenotrophomonas maltophilia yg3 can remarkably increase the number of effective leaves, the plant height and the biomass of the hot peppers, and the stenotrophomonas maltophilia yg3 can be applied to the field of microorganisms. Particularly, the method has important application value in the fields of microbial plant growth promoters and green synthesis of IAA (indoleacetic acid).

The invention discloses engineering chlamydomonas reinhardtii for synthesizing high-value pigment and a construction method and application thereof.An engineering chlamydomonas reinhardtii strain Blup-BKT-CHYB-CHLC is preserved in the China General Microbiological Culture Collection Center on September 1, 2025, and the preservation number is CGMCC NO.46634. The content of astaxanthin in the constructed engineering chlamydomonas reinhardtii strain reaches 25.5 mg / L, and the content of astaxanthin in the engineering chlamydomonas reinhardtii strain reaches 9.5 mg / L; in addition, the light energy utilization efficiency is remarkably enhanced, the stress resistance is improved, very strong oxidation resistance is achieved, the accumulation of astaxanthin endows the engineering strain with highlight tolerance, the yield of high-value pigments is increased, and the biomass of the engineering strain is greatly superior to that of a wild strain under the highlight condition. In addition, the chlorophyll c synthase is applied to the reconstruction of a pigment system of the chlamydomonas reinhardtii for the first time, a frontier tool is quickly converted, and a molecular mechanism of'chlorophyll c-astaxanthin-blue pigment 'for synergistically regulating the photosynthetic efficiency of the chlamydomonas reinhardtii and product synthesis is clarified for the first time. The research blank of pigment system coupling regulation and control of photosynthesis and metabolism in the chlamydomonas reinhardtii is filled.

The invention relates to a monoclonal antibody DIDA55 aiming at hog cholera virus, and a preparation method and application thereof, belonging to the field of medical preparations. The monoclonal antibody DIDA55 or an antigen binding fragment thereof comprises a heavy chain variable region and a light chain variable region, amino acid sequences of LCDR1, LCDR2 and LCDR3 in a light chain variable region of the antibody are shown as 24th to 34th, 50th to 56th and 89th to 97th in SEQ ID No: 4; amino acid sequences of HCDR1, HCDR2 and HCDR3 in a heavy chain variable region of the antibody are shown as the 31st to 35th, the 50th to 65th and the 95th to 102th of SEQ ID No: 5. The monoclonal antibody provided by the invention can be used in the fields of swine fever E2 protein labeled subunit vaccine immunity, serological diagnosis of wild strains, swine fever vaccine immune effect evaluation, related experiments and the like, and provides antibody resources for prevention, control and purification of CSF.

The invention discloses kluyveromyces marxianus and application of the kluyveromyces marxianus in production of single-cell protein by taking straw as a raw material. The Kluyveromyces marxianus is a ZH-2 bacterial strain which is CGMCC (China General Microbiological Culture Collection Center) No.32295 The strain is obtained from a wild strain in a laboratory adaptive evolution mode, wheat straw and an inorganic nitrogen source can be used as raw materials for efficiently producing single-cell protein, compared with a parent strain, the utilization speed and the utilization rate of biomass, glucose and xylose are remarkably increased, the yield of ZH-2 SCP can reach 0.047 g.g <-1 >, and the yield of ZH-2 SCP can reach 0.045 g.g <-1 >. And the essential amino acid mode basically accords with adult and infant amino acid modes, and can be used as high-quality protein nutrition supply. The Kluyveromyces marxianus ZH-2 strain capable of obtaining single-cell protein by fermenting wheat straw as a raw material is provided from the perspective of utilizing cheap waste resources, so that the production cost of SCP is greatly reduced, and the problem of protein resource shortage is effectively solved.

Provided are an Aeromonas dhakensis mutant strain with deletion of a surface polysaccharide synthesis gene, a complemented strain, a construction method therefor, and the use thereof. The mutant strain is obtained by deleting one or more of ugd, ugd2, and cap1J from the Aeromonas dhakensis wild strain A. dhakensis C160501.

The invention relates to the field of microorganisms, in particular to bacillus velezensis and application of the bacillus velezensis in preparation of surfactin, the preservation number is CCTCC NO: M 20251689, the bacillus velezensis is a wild type bacillus velezensis strain, and the surfactin yield of the bacillus velezensis is 2-3 times higher than that of a conventional wild strain. At the shake flask level, the yield of the surfactin reaches 10.4 g / L, when fed-batch fermentation is carried out in a 5L fermentation tank, the yield reaches 22.3 g / L, the yield and production intensity are higher than the currently known wild fungus fermentation level, and the method has huge industrial application potential.

The invention provides a nucleic acid probe, a primer group, a kit, a detection method and application, and relates to the technical field of biology. The nucleotide sequence of the nucleic acid probe is as shown in SEQ ID NO. 1; wherein the 3'end of the nucleic acid probe is modified with MGB, and locked nucleic acid is modified at a base position corresponding to the difference between the swine fever wild strain and the swine fever vaccine strain in the nucleotide sequence. According to the nucleic acid probe, a specific sequence is utilized to precisely target a swine fever wild strain, and locked nucleic acid modification is introduced at a differential site, so that the recognition capability on single-base mismatch is remarkably enhanced, non-specific binding to a vaccine strain is thoroughly blocked, and false positive interference is eliminated; and 3 '-terminal MGB modification is matched to improve the binding stability and sensitivity, so that the efficient and accurate identification of the swine fever wild strain under the immune background is ensured.

The invention relates to the technical field of agricultural biology, in particular to application of a regulatory gene Mt2312847 in improving the cellulase expression quantity of mycelia thermophila. Through interference or knockout of a regulatory gene Mt2312847 of a wild strain of the mycelia thermophila and knockout of the strain (Mt delta 2312847), the cellulase concentration of fermentation liquor of the interference strain is higher than that of the wild strain, and the CMCase enzyme activity of the Mt delta 2312847 and the CMCase enzyme activity of the Mt delta 2312847 are 2.2 times and 1.6 times that of a control strain.

The invention discloses recombinant xanthomonas campestris with xanthan gum hydrolase displayed on the surface and a construction method and application of the recombinant xanthomonas campestris. The recombinant xanthomonas campestris for surface display of xanthan hydrolase is constructed by introducing a recombinant vector obtained by fusion expression of an ice crystal nuclein (INP) gene and a xanthan hydrolase gene into xanthomonas campestris. The gel yield of recombinant xanthomonas campestris fermentation is twice that of a wild strain and reaches 6.7 g / L. The strain can efficiently hydrolyze xanthan gum with high molecular weight (such as more than 100 wDa), the traditional production process which is relatively complicated is simplified, and a new method is provided for large-scale production of the xanthan gum with low molecular weight.

The invention relates to construction and application of an engineering bacterium for high yield of chitinase ChiA, in particular to a bacillus thuringiensis engineering bacterium HD73 delta cdsR (P5014-SPchiD-chiA) strain, and the preservation number of the bacillus thuringiensis engineering bacterium HD73 delta cdsR (P5014-SPchiD-chiA) strain is CGMCC (China General Microbiological Culture Collection Center) No.35677. The HD73 endogenous gene chiA gene is efficiently expressed in the bacillus thuringiensis delta cdsR, and the yield in a fermentation culture medium is far higher than that of a wild strain HD73. Compared with HD73 (P5014-SPchiD-chiA), the compound has a relatively strong inhibition effect on mycelial growth of fusarium graminearum and exserohilum turcicum, and shows a better effect on prevention and treatment of larvae of plutella xylostella compared with HD73 (P5014-SPchiD-chiA).

The application belongs to the technical field of biotechnology, and particularly relates to a human metapneumovirus mouse-adapted strain and application thereof. Compared with wild strains, the strain can exhibit stronger susceptibility to animals, and can cause typical clinical symptoms and even death. For example, after the strain infects BALB / c mice, the disease symptoms are obvious, 83% of the mice have a severe decrease in body weight to below 75% after infection, and have reached the humane killing standard. Four days after the strain infects BALB / c mice, the mortality rate is above 50%. The strain can be used to construct a cell model or an animal model of human metapneumovirus infection or a disease caused by the human metapneumovirus, and is further used to screen drugs for preventing and / or treating human metapneumovirus infection or a disease caused by the human metapneumovirus. In addition, the strain can also be used to prepare drugs for preventing and / or treating human metapneumovirus infection or a disease caused by the human metapneumovirus, and to prepare antibodies or antisera against the human metapneumovirus.

The present invention discloses the application of miRNA derived from insect vectors in inhibiting rice stripe virus infection, belonging to the field of biotechnology. The miRNA is the following miRNA A1) and A2): A1) a single-stranded RNA molecule having a nucleotide sequence of SEQ ID No.1; A2) a miRNA obtained by replacing and / or deleting and / or adding nucleotides of the nucleotide sequence shown in SEQ ID No.1, which has more than 90% identity with the RNA molecule shown in A1) and is related to plant resistance to rice stripe virus. The present invention has experimentally demonstrated that the NP content in rice overexpressing miR-263a is significantly lower than that in wild strains, indicating that overexpressing miR-263a can inhibit the proliferation of RSV virus in plants.

The invention discloses an amylosucrase mutant and application thereof in synthesis of alpha-arbutin, the mutant is obtained by mutation of an amino acid sequence of amylosucrase derived from coprophilobacterium mediterranei, and the amino acid sequence of the mutant is shown as SEQ ID NO.4. The invention further discloses a preparation method of the amylosucrase mutant. Compared with a wild strain, the catalytic activity, the temperature stability and the substrate tolerance of the amylosucrase mutant disclosed by the invention are remarkably improved; the recombinant bacteria constructed by using the recombinant bacteria can be used as a whole-cell catalyst to efficiently catalyze a substrate to synthesize alpha-arbutin in a reaction system containing hydroquinone. Therefore, the amylosucrase mutant disclosed by the invention has a good application prospect.

The invention relates to the technical field of biological medicines, and discloses a construction method and expression application of an Hcp gene deleted strain of a rahnella aqua KCL-5 strain for fish. The Hcp 0186 gene deletion strain is constructed by using a homologous recombination method, the comparison between the deletion strain and a wild strain is carried out, and the result shows that the deletion of the Hcp 0186 gene has an influence on the growth speed, the pathogenicity of the Hcp 0186 gene to the zebra fish is obviously reduced, the survival rate of the infected zebra fish is increased from 0% to 52%, and the Hcp 0186 gene deletion strain can be used as a fish attenuated live vaccine. A recombinant prokaryotic expression vector pET32a-Hcp and expression and purification of fusion protein of the recombinant prokaryotic expression vector pET32a-Hcp are further constructed, and Hcp 0186 rabbit polyclonal antibody serum is prepared as a primary antibody and can be applied to localization expression analysis of fish tissues infected by the bacterium and detection of WB and IHC. Results prove that the Hcp 0186 gene deletion of the strain provides an attenuated strain vaccine and clinical diagnosis and detection application of infected fish of the attenuated strain vaccine, and a scientific basis can be provided for revealing a molecular pathogenic mechanism of the strain and development and application of a mutant strain of the strain.

The invention relates to the technical field of virus detection, in particular to a fluorescent RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection probe 1106VP and / or 1106WP and primers 1106VWF and 1106VWR. The invention also discloses a fluorescent RT-PCR detection reagent, a fluorescent RT-PCR detection kit, a fluorescent RT-PCR detection probe, a primer, the fluorescent RT-PCR detection reagent and the application of the fluorescent RT-PCR detection kit in identification of a porcine reproductive and respiratory syndrome NJ-1106R strain and a porcine reproductive and respiratory syndrome non-NJ-1106R strain. The probe and the primer disclosed by the invention are high in specificity and high in sensitivity; the accuracy is high, and the accuracy rate of blind sample detection is 100%; the kit is high in universality, can be used for detecting virus liquid for cell culture in a laboratory, and can also be used for detecting porcine serum and tissue samples in clinical samples. The method is an effective method for identifying the PRRSV NJ-1106R vaccine strain and other PRRSV wild strains.

The invention relates to construction and application of an engineering bacterium for high yield of chitinase ChiD, in particular to a bacillus thuringiensis engineering bacterium deltacdsR (P5014-chiD) strain, and the preservation number of the bacillus thuringiensis engineering bacterium deltacdsR (P5014-chiD) strain is GGMCC No.35124. The HD73 endogenous gene chiD gene is efficiently expressed in the bacillus thuringiensis delta cdsR, and the yield in a fermentation culture medium is far higher than that of a wild strain HD73. Compared with HD73 (P5014-chiD), the compound has a relatively strong inhibition effect on mycelial growth of fusarium graminearum and exserohilum turcicum, and shows a better effect on prevention and treatment of larvae of plutella xylostella compared with the HD73 (P5014-chiD).

The application discloses a portable gradient freeze-drying preservation device for wild strain germplasm resources, and relates to the technical field of wild fungus preservation.The application comprises a preservation box, a box cover is rotationally matched with the preservation box, a trapezoidal wild fungus placing rack is slidably matched in the preservation box, and a plurality of universal wheels are arranged at the bottom of the preservation box;an elevating assembly connected with the trapezoidal wild fungus placing rack is arranged on one side in the preservation box, the elevating assembly is matched with the box cover, and an intelligent preservation system is arranged in the preservation box.A temperature gradient control unit is arranged in the application, and a precise controllable temperature gradient can be formed in different level areas of the trapezoidal wild fungus placing rack according to the optimal preservation temperature requirements of different wild fungi.For example, the upper area of the placing rack can be set as a relatively high temperature interval to adapt to some wild fungi which prefer warm environment.

The invention relates to the technical field of virus detection, and particularly discloses a fluorescent RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection kit for identifying a classical swine fever virus vaccine strain and a wild strain. The kit comprises a specific primer combination, a specific fluorescent probe, a fluorescent RT-PCR (Reverse Transcription-Polymerase Chain Reaction) reaction solution, a positive control and a negative control, the primer combination comprises a vaccine strain and wild strain specific primer pair targeting a virus 5 '-UTR region and an E2 gene; the fluorescent probes comprise probes corresponding to the targets respectively and are marked with fluorophores; verification is carried out through double gene targets, missing detection caused by single site mutation is effectively avoided, and the method has the advantages of high specificity, high sensitivity and high accuracy; the invention further provides a non-diagnostic purpose detection method of the kit, detection can be completed within 2 h, and the kit is suitable for rapid identification, epidemic situation monitoring and purification effect evaluation of classical swine fever virus vaccine strains and wild strains in clinical samples.

The application belongs to the field of bioengineering and particularly relates to a Bradyrhizobium elkanii with improved nodulation capacity and a construction method thereof. The pip gene of the Bradyrhizobium elkanii FG-486 is knocked out to improve the nodulation capacity of the Bradyrhizobium elkanii FG-486, and the Bradyrhizobium elkanii FG-486 delta P with improved nodulation capacity is obtained. Compared with the wild strain FG-486, the average nodulation number of soybean plants after seed dressing with the Bradyrhizobium elkanii FG-486 delta P is increased by 31.15%, the root nodule dry weight is increased by 10.26%, and the soybean yield is increased by 7.85%.

The invention discloses a pharmaceutical composition for preventing and treating novel coronavirus infection, the pharmaceutical composition comprises a phthalate compound, and the phthalate compound has a structure as shown in formula I (formula I); in the formula I, R1 and R2 are the same or different and are independently selected from substituted or unsubstituted straight chain or branched chain alkyl with the carbon atom number of 1-20. The pharmaceutical composition takes a phthalate compound as an active ingredient, cell membrane fusion mediated by combination of S protein of the novel coronavirus SARS-CoV-2 and a target cell receptor ACE2 is blocked, the inhibition effect on SARS-CoV-2 virus infection is achieved, in addition, the phthalate compound not only has a remarkable inhibition effect on SARS-CoV-2 wild strains, but also has a remarkable inhibition effect on the SARS-CoV-2 wild strains. And the SARS-CoV-2 mutant strain also shows a broad-spectrum antiviral effect, and is suitable for intervention of early, mild and asymptomatic novel coronavirus infection.

The application discloses a colloidal gold test strip for distinguishing between wild toxin infection and vaccine immunization of porcine pleuropneumonia actinobacillus and application. The test strip of the application takes ApxIVA truncated protein with an amino acid sequence shown as SEQ ID NO. 3 as an antigen, the truncated protein can be soluble expressed in E. coli, and the structure and function of the truncated protein are close to those of natural apxIVA. The test strip of the application comprises a sample pad, a colloidal gold pad, an NC membrane, a water absorption filter paper and a bottom plate, the colloidal gold pad is coated with gold-labeled apxIVA truncated protein and gold-labeled chicken IgY antibody, and the C line and the T line of the NC membrane are fixed with goat anti-chicken IgY antibody and apxIVA truncated protein. The test strip only needs 25 muL of pig serum to make detection within 5-10 min, and can distinguish between APP wild strain infection and gene deletion vaccine APP-HB-04M immunized serum samples.

The invention belongs to the field of improvement of acid tolerance and high-density fermentation of probiotics, and particularly relates to a domestication method for improving acid tolerance and fermentation density of lactobacillus plantarum and an obtained strain. The invention provides a domestication method for improving acid tolerance and fermentation density of lactobacillus plantarum, which is characterized in that Lactiplantarum ZJ316 (CCTCC NO: M 208077) is used as a wild strain, the wild strain is domesticated, and the obtained domesticated strain ZJ316PV (Lactiplantarum ZJ316PV) has the preservation number of CGMCC NO.33082. The acclimatization method for improving acid tolerance and fermentation density of lactobacillus plantarum has the advantages that the acclimatization method comprises the following steps: taking Lactiplantarum ZJ316 (CCTCC NO: M 208077) as the wild strain; the acid tolerance of the domesticated strain ZJ316PV is superior to that of the wild strain ZJ316. The invention also provides a method for improving the PV fermentation density of the wild strain ZJ316 and the domesticated strain ZJ316.

The invention belongs to the technical field of veterinary vaccines, and particularly relates to an orf virus ORF114 or ORF117 gene deleted strain as well as a preparation method and application thereof. The orf virus ORF114 gene deleted strain is obtained by deleting ORF114 on the basis of an orf virus wild strain, and the orf virus ORF117 gene deleted strain is obtained by deleting ORF117 on the basis of the orf virus wild strain. According to the preparation method, on the basis of an ORFV-CF15 strain (ORFV 2015 strain), ORF114 and ORF117 genes of the ORFV-CF15 strain are deleted by using a gene recombination combined Cre-LoxP system, and two orf gene deleted strains are constructed and screened, and the orf virus ORF114 or ORF117 gene deleted strains have the characteristics of low toxicity, good proliferation and enhancement of Th1 type immune response, and can be used as orf gene engineering vaccine candidate strains.

The present specification discloses a method for producing a peptide such as glutathione, and a microorganism that can be used by the method. One or more embodiments of a first aspect pertain to a method for producing a peptide such as glutathione, the method comprising culturing a prokaryotic microorganism strain in which the expression level of one or more genes selected from the group consisting of gshA genes, gshB genes, and gshF genes has been increased than that of a wild strain in a culture medium in which the total concentration of cysteine and cystine is 0.5 g / L or less. The second aspect relates to a microorganism in which the gamma-glutamyltransferase gene and the glutathione reductase gene are deleted and the expression of the gshA gene and the gshB, or gshF gene is enhanced.

The invention belongs to the technical field of gene engineering, and particularly relates to aureobasidium pullulans with high yield of pullulan and application of the aureobasidium pullulans. On the basis of aureobasidium pullulans M233-20, an isopullulanase gene A9187 gene in a genome of the aureobasidium pullulans M233-20 is knocked out based on a homologous recombination technology, and through polysaccharide fermentation test detection, an isopullulanase gene deletion mutant strain aureobasidium pullulans delta A9187-1 with the pullulan polysaccharide molecular weight improved and the yield not reduced is obtained. The weight-average molecular weight of the pullulan produced by the recombinant strain is 6.5 * 10 < 5 > Da, which is 2.6 times that of the original wild strain (2.46 * 10 < 5 >), and the yield of the pullulan is increased from 147.33 g / L to 156 g / L, which is increased by 5.88%.

The invention belongs to the technical field of genetic engineering, and particularly relates to hidden plasmid mediated genetic engineering probiotics as well as a construction method and application thereof. According to the method, stable genetic expression of exogenous genes is mediated through high-copy hidden plasmids, resistance does not need to be added in the culture process, and the safety, stability and effectiveness of synthesis of food-grade nutritional chemicals are remarkably improved. The modified strain can efficiently utilize glucose as a carbon source to synthesize a functional nutritional chemical VB5 product, the titer of VB5 in shake flask fermentation reaches 102 mg / L, and the product is not detected by a wild strain. The invention provides a new strain and technical support for food-grade efficient synthesis of functional nutritional chemicals, and is beneficial to popularization and application in the field of food.

The present invention belongs to the technical field of biopharmaceuticals and specifically relates to use of a pharmaceutical composition in combating mycobacterial infection. The use of zuclopenthixol in combination with bedaquiline exhibits significantly enhanced anti-mycobacterial infection activity. Compared to the use of bedaquiline alone, the use of 2 μg / ml zuclopenthixol in combination with bedaquiline results in varying reductions in colony counts of the MmpL5-MmpS5 strain when the concentration of bedaquiline ranges from 1 / 16 MIC to 2 MIC. As an enhancer of bedaquiline, zuclopenthixol can enhance the anti-mycobacterial infection activity of bedaquiline. The minimum inhibitory concentration of zuclopenthixol against the Rv0678 mutant strain in vitro is 16 μg / ml, and the minimum inhibitory concentration of BDQ against the Rv0678 mutant strain in vitro is 1 μg / mL. The use of 1 μg / ml zuclopenthixol in combination with BDQ reduces the MIC of BDQ against the Rv0678 mutant strain from 1 μg / ml to 0.03 μg / ml (a 32-fold increase in efficacy), which is lower than the MIC against the wild strain H37Rv (0.06 μg / ml), reversing resistance to BDQ and further enhancing the anti-mycobacterial infection activity.

The invention discloses a sugar-inducible promoter and application thereof in prevention and treatment of monochamus alternatus, and relates to the technical field of genetic engineering and biological prevention and treatment. Based on the technical background of transgenic symbiotic bacteria in prevention and treatment of monochamus alternatus, the scheme has the advantages that the transgenic symbiotic bacteria and wild strains have a competitive relationship in ecological niche; under high-level expression driven by a constitutive promoter, the transgenic symbiotic bacteria may be too greedy in resource utilization, so that the transgenic symbiotic bacteria conflict with wild strains and even are eliminated by the wild strains; according to the scheme, expression of Cry3Aa-T protein is regulated and controlled by using the sugar-induced promoter so as to reduce the cost of fitness of exogenous genes carried by serratia marcescens, and a hidden and effective method is provided for preventing and controlling propagation of vector insect monochamus alternatus.

The application discloses a humanized anti-SARS-COV-2 virus monoclonal neutralizing antibody, the neutralizing antibody is screened by single B cell flow sorting-antibody gene amplification pairing expression technology, has a unique CDR region, can be specifically combined with SARS-COV-2 and can effectively neutralize SARS-COV-2 wild strains and Omicron BA.5, Omicron XBB.1.5, Omicron EG.5, Omicron JN.1, Omicron KP.2 and other Omicron mutant strains, the IC50 is lower than 0.1 μg / mL, has broad-spectrum virus neutralization activity, and the application also relates to application of the antibody in preparation of a new coronavirus disease treatment or prevention drug and a detection reagent, the antibody has the characteristics of full humanization, high expression and good stability, and is suitable for industrialization.