1327 results about "Shake flask" patented technology

Human/human hybrid cells were made via fusion of human embryonic kidney cells (293S) and modified Burkitt's lymphoma cells (2B8). The fusion cells are useful as host cells for the recombinant expression of mammalian genes. The advantages of using these hybrid clones of human kidney- and B-cells, called HKBs, for mammalian gene expression, include (i) the cells are negative for immunoglobulin expression, (ii) the cells grow easily in plasma protein-free medium (with or without the addition of recombinant insulin) as suspension cultures in a shake flask or in a fermenter (iii) the cells are very susceptible for transfection of DNA, and (iv) the cells secrete high levels of heterologous recombinant proteins, such as recombinant monoclonal antibodies, soluble ICAM-1, rIL-4, and rFVIII.

The present invention provides one kind of gamma-polyglutamic acid generating bacterium and the process of preparing gamma-polyglutamic acid (gamma-PGA) therewith. The gamma-polyglutamic acid generating bacterium is Bacillus substilis CCTCC M 206102. The preparation process of gamma-PGA includes using Bacillus substilis CCTCC M 206102 as fermenting strain, and shake flask fermentation of sugar material without adding L-glutamic acid. The present invention has relatively low material cost, gamma-PGA output up to 18g/L and excellent industrial application foreground.

A reaction vessel assembly achieves enhanced aeration or gas exchange in its gas-in-liquid multiphase mixing reaction through the use of its mixing energy and gas-lift venting, and without the traditionally practiced external line gas sparging. It combines the best of both shake flask and stirred tank reactor vessel and possesses some of the key generic features like cost and parallel experiment advantages of the traditional flasks and shaker hybrid but without their shortcomings like limited gas exchange and uncharacteristic mixing.

A marine-derived Bacillus velezensis (Bacillus velezensis) BMF 03 having a deposit number of CCTCC NO: 2017374 is disclosed. BMF 03 strain and its aseptic fermentation broth have inhibitory effect onplant pathogenic fungi, including apple rot fungi, grape white rot fungi, mango anthracnose fungi and so on. The aseptic fermentation broth of BMF 03 strain can be used as an active ingredient to prepare an antibacterial drug, and the bacteria is selected from the group consisting of Keratinobacteria, Bacillus subtilis and Ralstonia solanacearum. The invention also discloses a fermentation methodof aseptic fermentation broth of BMF 03. The invention adopts a plate confrontation method and an Oxford cup method to screen a new marine bacterial strain BMF-03 with strong bacteriostatic effect andwide bacteriostatic spectrum, and adopts strain morphological observation, physiological and biochemical tests and 16S rDNA sequence analysis to determine the species relationship of excellent resistant strains. At that same time, the bacteriostatic substance produce by the strain, the sporulation fermentation medium and the shake flask fermentation conditions were also study, and the promoting effect of the strain fermentation broth and the solid fermentation product on the growth of the cucumber seedling was clarified, which laid a foundation for the application of the strain.

The invention provides a method for improving the quality of expanded cut rolled stem of tobacco by microbial enzyme. The procedures are as follows:1) Preparation of bio-enzyme: (1) Aspergillus niger is activated and cultured by potato cane sugar culture medium and is then transferred into sterilized seed culture medium, and shake cultivation is carried out at the temperature of 28 DEG C for 24 hours with 250r/min<-1>; seed liquid with 10% of inoculation amount is transferred into a sterilized 500ml shake flask (containing 100ml culture liquid for enzyme), and the seed liquid carries out incubation for culturing at the temperature of 25 DEG C for 96 hours and then zymotic liquid is obtained. (2) Extraction of lignin degradation complex enzyme: After being filtered by filter paper, the zymotic liquid is centrifugally separated at the low speed of 1500rpm. The supernatant carries out fractional salting out by (NH4)2SO4 till the saturation is 0.7 to obtain crude enzyme liquid, after the crude enzyme liquid carries out ultrafiltration dialysis, the lignin degradation complex enzyme is obtained. Activities of enzyme components: 350U/L of Lip, 11U/L of MnP and 5.6U/L of Lac; 2) Improvement of the quality of expanded cut rolled stem of tobacco: The expanded cut rolled stem for cigarette is weighed and sprayed evenly by lignin degradation complex enzyme liquid containing 0.2-0.4% of expanded cut rolled stem after being diluted by distilled water at the temperature of 27 DEG C-29 DEG C. The expanded cut rolled stem of tobacco is placed in a sealed container for enzymolysis for 95-97 hours and dried naturally till the moisture content is 12.5%-15%. The result by subjective analysis suggests that the complex enzyme preparation can effectively decrease the lignin content of the expanded cut rolled stem, promote the transformation of aroma matter and improve aroma quality and taste.

An elastomeric article having reducing microbe affinity and transmission and methods for applying and immobilizing antimicrobial compounds to the elastomeric substrate surface are disclosed. The elastomeric article has a body formed of a natural or synthetic polymer latex having an outer surface and an inner surface. The body has a coating of an antimicrobial agent over at least a portion of said outer surface. The treatment involves applying according to either a spraying or dipping process an antimicrobial polymer or composition to a surface of the elastomeric substrate; binding the antimicrobial composition to the surface in a manner such that said treat antimicrobial coating passes either one or another or both versions of a zone of inhibition test, such test including: a) a dry-leaching or agar-plate-based contact test, according to AATCC 147 protocol, or b) a wet-leaching or dynamic shake flask test according to ASTM E-2149-01 protocol. The substrate is further subject to a rapid germicidal contact-transfer test of relatively short duration. The antimicrobial polymer can include an organosilane quaternary ammonium or a biguanide compound which can disrupt the ionic charges of microbial cellular membranes.

The invention discloses a high-yield strain streptomyces lydicus, as well as breeding and fermentation thereof. The fermentation of the high-wield stain streptomyces lydicus E9 CGMCC No.3075 comprises the following steps: (1) preparation of a shake flask, seeds and a fermentation medium; (2) shake flask culture; (2) seed culture; (4) fermentation pot culture: leading a seed culture liquid in a fermentation pot filled with the fermentation medium and culturing for 24-96 hours to obtain a high-wield stain streptomyces lydicus E9 fermentation liquor under the condition of ventilating, stirring, temperature of 25-35 DEG C, and pH of 4-9; and carrying out ultrasonication for 20-40 min. The strain of the invention can generate large quantity of substances with antimycotic activity in the fermentation liquor, and the fermentation liquor has evident inhibiting effect on rhizoctonia solani; and the bacterial inhibition rate of the fermentation liquor which is diluted by 50 times reaches above 97.8% after 48 hour fermentation.

This invention provides a novel methods and devices for measurement of particle concentration or changes in particle concentration over a wide linear range. The invention comprises one or more radiation sources and one or more detectors contained in a housing which is interfaced to a medium containing particulate matter. The one or more radiation sources are directed into the medium, scattered or transmitted by the particulate matter, and then some portion of the radiation is detected by the one or more detectors. Methods for confining the measurement to a specific volume within the medium are described. Algorithms are provided for combining the signals generated by multiple source-detector pairs in a manner that results in a wide linear range of response to changes in particle concentration. In one embodiment the sensor provides non-invasive measurements of biomass in a bioreactor. In another embodiment an immersible probe design is described, which may be suited for one-time use. In an addition embodiment, a sensor is provided which is well suited to the rapid sequential measurement of particle concentration in multiple vessels, such as assessment of biomass in series of shake flasks.

The present invention discloses a strain capable of producing poly-gamma-glutamic acid and a method for producing poly-gamma-glutamic acid by utilizing said strain. The described strain is X-003, a Bacillussubilis, CCTCC, NO:M202048. It adopts seed liquor preparation and liquid submerged fermentation process to produce and obtain the poly-gamma-glutamic acid product. The hereditary feature of said strain is stable, and its capacity for synthesizing poly-gamma-glutamic acid is strong, and the molecular weight of synthetic poly-gamma-glutamic acid can be up to above 10000 KD. The shake-flask fermentation level of poly-gamma-glutamic acid by utilizing said invented strain and its fermentation method can be up to 10 mg/ml, and the fermentation level of the fermentation tank with 80L is 30 mg/ml.

The invention relates to a preparation method of rhamnolipid biological fermentation liquid. The method comprises the following steps: extracting and purifying Pseudomonas aeruginosa obtained from petroleum-contaminated soil, and obtaining Pseudomonas aeruginosa VTS-1 (Pseudomonas sp. -1) The preservation number is: CGMCC2200, and the specific fermentation and cultivation steps are as follows: A. Strain VTS-1 is cultured in shake flasks; B. Primary fermentation cultivation; C. Secondary fermentation cultivation; D. Fermentation cultivation. The process of the method has the advantages of high yield, high efficiency, high loading coefficient, short fermentation cycle, simple process, reduced overall product cost, and is completely suitable for industrial production, and has played a role in the promotion and large-scale application of rhamnolipid biosurfactants. important role.

The invention discloses an acclimatization and screening method for heterotrophic nitrification aerobic denitrifying bacteria. According to the invention, the heterotrophic nitrification aerobic denitrifying bacteria are obtained through shaking flask enrichment, sludge accumulation, SBR reactor batch acclimation and low-DO (Dissolved Oxygen) process gradual acclimation and isolation and screening, and a method for efficiently acquiring target strains is established. According to the screening method of the invention, the heterotrophic nitrification aerobic denitrifying bacteria can be successfully screened, further, microbial preparation prepared with the screened bacteria as active components can be used for effectively depriving ammonia nitrogen and total nitrogen in the water, meanwhile, and the preparation has tolerance and degradation ability for phenols, amines, heterocyclic materials, cyanogens and polyaromatic hydrocarbons and other toxic materials in waste water, has strong accommodative ability and has great application prospect in actual engineering application.

Screening and application of yeast with high ethanol yield and low fusel oil yield in Chinese Maotai-flavor liquor production belong to the technical field of biological engineering. The strain is any one strain selected from pichia anomala CGMCC4740, schizosaccharomyces pombe CGMCC4744, zygosaccharomyces bailii CGMCC4745, trichosporon asahii CGMCC4746, saccharomyces cerevisiae CGMCC4747, kazachstania exigua CGMCC4748, pichia fermentans CGMCC4750. The strain is separated from various Daqu, fermented grains, brewing raw materials and brewing environment of several Chinese famous distilleries; the screening method comprises the following steps: diluting distiller yeast samples, fermented grains, brewing raw materials or brewing environment substances, coating on a TTC primary screening plate, selecting a colony with an obvious red color, inoculating the colony in a shake flask for culture, performing secondary screening, determining the contents of ethanol and fusel oil of the fermentation broth by a distillation specific gravity method and HS-SPME-GC-MS respectively, screening the strain with high ethanol concentration and low fusel oil concentration. The strain is applicable to food industry and brewed wine industry.

The invention relates to an artificial cultivation method for Taiwan cordyceps fruiting bodies. Hirsutellahuangshanensis is adopted as a strain. The detailed cultivation operation comprises the following steps: A, activating and cultivating the strain in a test tube with an oblique surface; B, cultivating seeds in a shake flask filled with liquid; C, cultivating mycelia; D, and cultivating fruiting bodies to obtain artificially cultivated Taiwan cordyceps fruiting bodies which are rod-shaped and are orange yellow or orange red in color, wherein the length and the dimension of the artificially cultivated Taiwan cordyceps fruiting bodies are similar to the length and the dimension of wild Taiwan cordyceps fruiting bodies. The method is initiative; the Taiwan cordyceps fruiting bodies can be smoothly cultivated by using the method; the problem of the lack of wild Taiwan cordyceps resources is solved; and the method provides a broad prospect for comprehensive development, industrial production, medical use, edible use and further analysis, extraction and separation of active components of Taiwan cordyceps; moreover, the method has the advantages of adopting simple production process, realizing low cost, having wide sources of raw materials, leading to no pollution and being suitable for large-scale production.

The invention discloses a bacillus subtilis chitosanase as well as a preparation method and application thereof. The invention optimizes an encoding gene of bacillus subtilis chitosanase according to the preference of pichia pastoris codon. The optimized nucleotide sequence is shown as SEQ ID NO.2. A pichia pastoris expression system is further utilized to perform efficient secretory expression on the optimized chitosanase encoding gene, so as to obtain the bacillus subtilis chitosanase with the amino acid sequence as SEQ ID NO.1. The bacillus subtilis chitosanase obtained according to the invention has higher hydrolytic activity to chitosan substrates at different degrees of deacetylation; the crude enzyme generated through shake-flask fermentation has the hydrolysis capacity of degrading 5g of chitosan by 1mL crude enzyme (0.3mg of protein), about 150mg of non-specific commercial enzyme is required for degrading the same amount of chitosan, and the efficiency is theoretically increased by 500 times; and the bacillus subtilis chitosanase has excellent industrial application prospects.

The invention discloses a non-invasive biomass online detection device used for shake-flask culture. The device comprises a master control unit, a shake flask capable of containing biological turbid liquid and a fixing sleeve covering the outer side of the shake flask. A detection probe is connected to the fixing sleeve on the outer side of the bottom of the shake flask. The detection probe comprises a substrate capable of being attached to the bottom of the shake flask, a light source, a photoelectric sensor and an acceleration sensor, wherein the light source, the photoelectric sensor and the acceleration sensor are arranged on the substrate. The light source can generate a light path obliquely irradiated into the shake flask. The photoelectric sensor is located below the light path and used for measuring scattering light generated after the light path is irradiated to the biological turbid liquid. The light source, the photoelectric sensor and the acceleration sensor are all connected with the master control unit. According to the non-invasive biomass online detection device, a turbidity measurement method is adopted for performing online detection on the concentration of bacterial liquid cultured in the shake flask through a back scattering light measurement technology, and the concentration of the biological turbid liquid in the shake flask can be detected under the condition that culture equipment is not broken. The non-invasive biomass online detection device is simple in structure, low in cost, and capable of effectively improving detection efficiency and precision and avoiding sample contamination.

The invention discloses a cyclodextrin glucosyl transferase enzyme mutant, and belongs to the field of genetic engineering and enzyme engineering. The cyclodextrin glucosyl transferase enzyme is mutated to obtain a mutant having higher dismutation activity than that of the cyclodextrin glucosyl transferase enzyme. The shake-flask fermentation enzyme dismutation activities of mutants V6D, S90G, T168A, T171A, T383A, G608A and V6D/S90G/T168A/T171A/T383A/G608A are respectively 1.89, 1.21, 1.21, 1.22, 1.32, 2.03 and 3.16 times that of wild enzyme. The mutant has certain magnificence for industrialproduction of cyclodextrin glucosyl transferase enzyme, and the application potential of the enzyme in foods, medicine and chemical engineering industries can be improved.

The invention provides a gene engineer strain of Aspergillus niger with high L-malic acid yield and a construction method thereof. The method utilizes gene knockout technique to disrupt the Aspergillus niger oxaloacetic acid hydrolase gene (oahA) so as to obtain the malic acid-producing strain S2. After 7 days of shake flask fermentation, 100 g/L glucose is converted into 120.4 +/- 2. 8 g/L of L-malic acid, the conversion of malic acid and malic acid to glucose is 1.62 mol/mol, which is 81% of the highest conversion in theory. A good strain for the industrialized production of malic acid is provided.

The invention discloses a method for processing dye waste water based on activation of peroxymonosufate by three-dimensional ordered mesoporous CoFe2O4 and aims to solve the problem of low catalysis efficiency of present spinel CoFe2O4 to peroxymonosufate. The method is implemented through the following steps: firstly, three-dimensional ordered mesoporous CoFe2O4 is prepared; secondly, dye waste water with an appointed concentration is prepared, and the prepared solution is placed in a brown shake flask; thirdly, peroxymonosufate is added; fourthly, the three-dimensional ordered mesoporous CoFe2O4 is added; fifthly, the three-dimensional ordered mesoporous CoFe2O4 is separated by utilization of an applied magnetic field, and processing of dye in waste water based on activation of peroxymonosufate by three-dimensional ordered mesoporous CoFe2O4 can be completed. High-efficiency processing of dye waste water can be achieved by utilization of three-dimensional ordered mesoporous CoFe2O4 in cooperation with the peroxymonosufate technology, and the removal rate exceeds 90%. The cobalt ion leaching rate is low during the usage process, and environment pollution is reduced. The catalyst can be separated rapidly through an applied magnetic field and can be recycled, and the operation cost is lowered.

The present invention relates to a preparation method of a microecology fermented feed by utilizing byproducts of corn deep-processing as raw materials. The preparation method includes: 1, selecting three probiotics of bacillus subtilis, aspergillus oryzae and rhizopus, obtaining a second stage seed by slant culture, first stage seed culture in a shake flask and second stage culture in a shake flask, and carrying out complex formulation according to a certain proportion; 2, selecting three probiotics of lactic acid bacteria, candida utilis and flavor yeast, obtaining a second stage seed by slant culture, first stage seed culture in a shake flask and second stage culture in a shake flask, and carrying out complex formulation according to a certain proportion; 3, reasonably proportioning to prepare a fermentation medium by utilizing the by-products of the corn deep-processing, such as corn bran, embryo cake, corn steep liquor and gluten meal, as raw materials; 4, inoculating the formulated probiotics of the step 1 in a solid state fermentation medium to perform aerobic fermentation, inoculating the formulated probiotics of the step 2 to perform anaerobic fermentation after aerobic fermentation for a while, drying after the fermentation, to obtain the microecological feed. The preparation method has high comprehensive utilization efficiency. The microecological feed has high active proteins, lactic acid and functional small peptides, and is a high quality health care feed which is easily absorbed by body.

The invention discloses a streptomyces parvus and application thereof for preparing daptomycin. The strain is reserved in the CCTCC and has the reservation number of CCTCC NO: M2010136, and the reservation data is June 4, 2010. The method for preparing the daptomycin is as follows: a, taking streptomyces parvus CCTCC NO: M2010136 as a fermentation strain; b, strain cultivation: inoculating slope lawn into a shake flask seeding tank to cultivate to obtain shake flask seeding liquid; inoculating the shake flask seeding liquid into the seeding tank to be cultivated; inoculating the seeding tank culture solution into fermentation tank culture medium to be cultivated, and collecting fermentation liquor; carrying out feed supplement in the fermentation and cultivation process; and c, extracting a fermentation product. The fermentation technology provided by the invention has stable production capability, high fermentation unit and few fermentation byproducts by the pilot plant test and the experiment in a 10-ton fermentation tank, greatly lowers post-extracting difficulty and is suitable for industrial production.

The invention provides an Acetobacter xylinum Y05 strain and a method for preparing nanometer cellulose skin tissue repairing material with the same. The preparation method comprises the following steps that: Acetobacter xylinum Y05 (CCTCC M 207163) preserved on an inclined plane is made into a shake-flask seed and cultivated statically to obtain a nanometer cellulose membrane; and the well-cultivated membrane is separated, purified and then compounded with natural polysaccharide (chitosan, etc.) and protein (collagen, silk fibroin, etc.), so as to prepare the repairing material. The nanometer repairing material has good biocompatibility, flexibility and strength which are similar to human skin, as well as good plasticity and elasticity, can be suitable for various irregular wound surfaces, can keep moisture for a long time, and can be used as a skin substitute and medical dressings for burns, chronic skin ulcers and other skin injuries or defects in clinical practice.

A large-scale deep liquid fermenting process for preparing the mycelia of antrodia cinnamonea includes such steps as collecting the fungus of antrodia cinnamonea, liquid slant culture in shake flask, enlarging the culture, class-one seed culture, fermenting culture, and fermenting in (1000-5000)-L fermentor for 42 hr.