314 results about "Whole cell" patented technology

The invention discloses a biosynthesis system for high-yield 1, 6-hexamethylenediamine and application of the biosynthesis system, and belongs to the technical field of biology. According to the method, key genes which are excavated and optimized in a 1, 6-hexamethylenediamine biosynthetic pathway are co-expressed in a single cell module or a multi-cell module, and different enzyme elements expressed by the cell module are subjected to enzyme cascade reaction, so that 1, 6-hexamethylenediamine can be efficiently synthesized by taking caprolactam or 6-aminocaproic acid as a substrate; furthermore, after the carboxylic acid reductase MaCAR is mutated, the catalytic activity of the carboxylic acid reductase MaCAR can be remarkably improved, and the yield of the 1, 6-hexamethylenediamine is further improved; in addition, the biosynthesis system disclosed by the invention directly takes the cell module as a whole-cell catalyst, and an enzyme purification step is avoided, so that the biosynthesis system has the advantage of low cost.

The invention discloses an arginine decarboxylase diaA enzyme mutant and application thereof in preparation of butanediamine, and belongs to the field of bioengineering. According to the invention, rational charge overturning transformation is simultaneously carried out on a pentamer meridian oligomeric interface and a dimer latitudinal oligomeric interface, so that stable assembly and efficient catalysis of the decamer under the condition of neutral to alkaline pH (7.0-9.0) are realized. Wherein positive charges are introduced into a meridian interface to weaken electrostatic repulsion, and negative charges are introduced into a latitudinal interface to enhance dimer compactness and substrate transfer efficiency. The specific enzyme activity of the representative double mutant AdiAD110K / H736E at pH 8.0 is about 35 times that of a wild type, and the representative double mutant AdiAD110K / H736E keeps a complete decamer state in a pH range of 7.0-9.0. The yield of butanediamine is up to 145.9 g / L under the whole-cell catalysis of the mutant.

The invention relates to a photovoltaic module, the photovoltaic module comprises three battery string groups which are connected in series, each battery string group comprises four battery strings which are connected in parallel, each battery string comprises a plurality of fragmented battery pieces, and each fragmented battery piece is a quarter piece formed by cutting a whole battery piece; the three battery string groups which are connected in series are distributed along a first direction, and a plurality of fragmented battery pieces in the battery strings are distributed along a second direction; the heads and the tails of the battery strings are connected with the bus bars, the bus bars comprise first bus bars located at the two ends of the photovoltaic module in the second direction and second bus bars located in the middle of the photovoltaic module in the second direction, and the first bus bars are located on the back face of the photovoltaic module. According to the photovoltaic module, on one hand, one half of the fragmented battery piece in the battery string is replaced by one fourth of the fragmented battery piece, and on the other hand, the photoelectric conversion efficiency of the photovoltaic module is relatively high by enabling the first bus bar to be located on the back surface of the photovoltaic module.

Provided are S-adenosylmethionine synthetase mutants and the use thereof, belonging to the technical field of biological enzyme engineering. Site-directed combinatorial mutations are performed on positions 189, 200, 234, 266 and 371 of S-adenosylmethionine synthetase MAT to obtain a series of mutants with improved enzyme activity, wherein the specific enzyme activity of I189V / V266H and V266H / N371R is increased by more than 10 times relative to the parent. The yields of S-adenosylmethionine synthesized by whole cells of recombinant bacteria expressing mutants I189V / V266H / N371R, V266H / Q234N / N371R and I189V / V266H / Q234N / N371R all reach 1550 mg / L or more. The provided enzyme mutants have broad application prospects in the production of S-adenosylmethionine and the construction of genetically engineered microorganisms for S-adenosylmethionine.

The invention discloses a high-efficiency lysine decarboxylase mutant and an application of the high-efficiency lysine decarboxylase mutant in synthesis of pentamethylene diamine. A series of mutants including a single mutant (such as N218G, T222V, C244P and the like), a double mutant and a three mutant are obtained by carrying out multi-site mutation on wild type lysine decarboxylase (SEQ ID NO.2) from Klebsiella grimontii. The catalytic efficiency of the mutant is remarkably improved, and the activity of the mutant is improved by 5.0-10.2 times compared with that of a wild type enzyme. According to the method disclosed by the invention, the substrate conversion rate of 99% or above can be realized within 2 hours through an optimized process of constructing an expression vector and a genetically engineered bacterium, inducing expression of lysine decarboxylase and synthesizing 1, 5-pentanediamine by utilizing a whole-cell catalysis technology. Compared with a traditional chemical method and a natural enzyme method, the method disclosed by the invention has the advantages of simplified process, high catalytic efficiency, low production cost and the like, and is suitable for producing bio-based 1, 5-pentamethylene diamine and nylon 56 salt.

The invention discloses an ecDNA (ectodeoxyribonucleic acid) identification method based on scATAC-seq (scATAC-Seq) data. The ecDNA recognition method provided by the invention can be used for accurately detecting copy number amplification, breakpoint quantity and ecDNA feature quantity on the whole cell level. According to the method provided by the invention, the specific carried annular oncogene in the tumor can be identified, the distribution characteristics of the annular oncogene in the genome can be analyzed, and the overall composition of all annular DNAs in a single cell and the specific composition of the single annular DNA can be disclosed. In addition, according to the method, the existence of the circular DNA can be verified through a dyeing experiment, and the reliability of a detection result is ensured.

The invention discloses a mutant with improved catalytic activity of S-adenosylmethionine synthetase and application of the mutant, and belongs to the technical field of biological enzyme engineering. According to the present invention, the site 189, the site 234, the site 266 and the site 371 of the S-adenosylmethionine synthetase MAT are subjected to site-specific combinatorial mutation to obtain a series of mutants with improved enzyme activity, the I189V / V266H improvement is the most obvious, and the specific enzyme activity is 3.5833 + / -0.1373 U.mg <-1 >, which is improved by 1353.08% compared to the parent WT; and for a whole-cell catalysis system, the yield of S-ademetionine synthesized by the mutant I189V / V266H / Q234N / N371R reaches 1751.01 mg / L, and the increase is most obvious. The enzyme mutant provided by the invention has a wide application prospect in the production of S-adenosylmethionine and the construction of genetic engineering microorganisms of S-adenosylmethionine.

The invention discloses a method for separating and purifying gamma-aminobutyric acid from whole cells or enzymatic conversion liquid. The method comprises the following steps: removing thalli and large-particle impurities, denaturating, decolorizing and removing impurities, crystallizing for the first time, washing for the first time, crystallizing for the second time, washing for the second time and drying. According to the present invention, the thermal denaturation of the protein, the active carbon decoloration and the flocculation impurity removal are integrated, such that the solid-liquid separation frequency is reduced, the problems of difficult filtration and easy leakage of the powdered active carbon are solved, and the purity and the yield of the gamma-aminobutyric acid are significantly improved. In the whole separation and purification process, the pH value is not adjusted, the cost is saved, impurities are reduced, and the ion removal process is omitted. And collecting the primary crystallization mother liquor and washing liquor for secondary crystallization, so that the yield of the final product is greatly improved. According to the separation and purification method, the purity of the obtained gamma-aminobutyric acid is greater than 99%, the yield is greater than 92%, and the crystal light transmittance is greater than 99%. The method provided by the invention is simple, efficient and low in cost, and has a wide application prospect.

The invention provides a plasmid, an alpha-ketoglutaric acid engineering strain and a construction method and application of the alpha-ketoglutaric acid engineering strain, the strain is obtained by adopting a directional transformation method, transcriptional levels of katE, katG, sodB and grxA genes are up-regulated, a catR gene and a katA gene are heterologously expressed, a high-copy plasmid pETKG is carried, and the plasmid simultaneously expresses L-glutamate oxidase LGOX genes from Streptomyces sp and Chain A; the strain self-synthesized catalase eliminates H2O2 generated in the process of producing alpha-ketoglutaric acid through enzyme catalysis, engineering bacteria of L-glutamate oxidase LGOX expressed by pETKG plasmids are used for performing whole-cell catalysis on L-sodium glutamate to synthesize alpha-ketoglutaric acid, and the engineering strain is a high-level engineering strain capable of industrially and stably producing alpha-ketoglutaric acid.

The invention provides an application of inositol monophosphate in an enzyme catalysis reaction system for preparing inositol. The invention also provides a preparation method of the inositol, the starch is used as a substrate, glucan phosphorylase, glucose phosphate mutase, inositol-3-phosphate synthase and inositol monophosphate are added as enzyme catalysts to catalyze the substrate to generate the inositol, the inositol monophosphate is derived from Archaeoglobus fulgidus, the amino acid sequence is shown as SEQ ID NO: 12, or the amino acid sequence is shown as SEQ ID NO: 1, or the amino acid sequence is shown as SEQ ID NO: 2, or the amino acid sequence is shown as SEQ ID NO: 3, or the amino acid sequence is shown as SEQ ID NO: 4, or the amino acid sequence is shown as SEQ ID NO: 5, or the amino acid sequence is shown as SEQ ID NO: 6. The inositol monophosphate is derived from Thermococcus kodakarensis, and the amino acid sequence of the inositol monophosphate is as shown in SEQ ID NO: 13; or the inositol monophosphate is derived from Pseudothermotoga lettinae, and the amino acid sequence of the inositol monophosphate is as shown in SEQ ID NO: 14. Inositol-3-phosphate synthetase and inositol monophosphate with good performance are screened, high-concentration starch is efficiently catalyzed to be converted into inositol by a one-pot method in a whole-cell feeding mode, and NAD < + > and other coenzymes do not need to be used. Under a 60L reaction system, 200g / L of corn starch is catalyzed, the unit yield of the prepared inositol reaches 161g / L, and the method has a good industrial application prospect.

The invention relates to the technical field of agricultural biology, in particular to a fusion P450 enzyme mutant, a method for improving VD3 catalytic activity and a method for generating 25 (OH) VD3 through whole cells. The invention constructs a fusion P450 enzyme VK1-CYP116B46-L21 and a self-sufficiency fusion P450 enzyme mutant with improved activity, wherein the self-sufficiency fusion P450 enzyme mutant is used for catalyzing vitamin D3 to synthesize calcifediol; vD3 is taken as a substrate, glucose, a cosolvent and a wet thallus induced by recombinant escherichia coli for simultaneously expressing fusion P450 enzyme and glucose dehydrogenase are added, and the highest yield of 25 (OH) VD3 within 24 hours after catalytic reaction can reach 3.26 g / L; whole cells are directly used as a catalyst, enzyme purification is not needed, glucose dehydrogenase is introduced, and glucose is used as a substrate to regenerate a cofactor NAD (P) H, so that addition of expensive cofactors is avoided, the production cost is reduced, and industrial production is facilitated.

The invention provides a photovoltaic module and a photovoltaic system. The photovoltaic module comprises a first bypass module, and a first battery string group and a second battery string group which are connected in parallel; each of the first battery string group and the second battery string group comprises two battery strings which are connected in parallel, each battery string comprises a plurality of quartered battery pieces which are connected in series, and each quartered battery piece is a quarter battery piece formed by cutting a whole battery piece; the photovoltaic module also comprises a second bypass module, and a first battery unit and a second battery unit which are connected in parallel. The first end of the first battery unit and the first end of the second battery unit are both connected with the output end of the second bypass module, and the second end of the first battery unit and the second end of the second battery unit are both connected with the input end of the second bypass module. According to the photovoltaic module provided by the embodiment of the invention, the battery piece is arranged to be the quarter battery piece formed by cutting the whole battery piece, so that the internal power loss of the battery piece can be reduced, and the output power of the photovoltaic module can be improved.

The invention discloses a method for producing MK-7 through whole-cell regulation and control by using different time sequence type promoters, recombinant escherichia coli and application. According to the method, the time sequence type promoters are used for regulating and controlling expression of different key genes related to MK-7 synthesis. According to the invention, the time sequence promoter is used for whole-cell regulation and control to produce the vitamin MK-7 for the first time, dynamic regulation of the MK-7 is realized, and the production efficiency of the MK-7 can be remarkably improved by accurately regulating and controlling expression of key genes in a synthesis route of the MK-7 and optimizing an intracellular metabolic process. The promoter disclosed by the invention can regulate cell metabolism and enhance synthesis of MK-7 in different growth stages or under different environmental conditions by controlling the time and strength of gene expression. The MK-7 is produced by adopting the constructed recombinant escherichia coli obtained based on whole-cell regulation of the sequential promoter, and the yield of 14 g / L can be finally achieved in a 5L fermentation tank.

The invention discloses a method for preparing butanediamine and spermidine from whole cells, and belongs to the field of bioengineering. According to a rational copolymerization modification strategy, a meridian interface and a latitudinal channel interface of arginine decarboxylase are subjected to rational modification respectively or simultaneously, and a series of mutants are obtained. The optimal mutant AdiA H729D / E467K / H736E, which is subjected to double-interface synergistic modification, disclosed by the invention, keeps high activity in the whole neutral-alkaline range of pH (Potential of Hydrogen) of 7.0 to 9.0. The optimal pH of the arginine decarboxylase mutant is increased, the enzyme activity stability in a neutral pH range is improved, the arginine decarboxylase mutant is more suitable for the condition requirements of industrial microbial fermentation, and a foundation is laid for efficient synthesis of butanediamine. The spermidine yield is up to 153.3 mg / L by using the mutant to catalyze the whole cell.

The invention relates to a photovoltaic module and a production method of the photovoltaic module, and a whole battery piece can be cut into four split battery pieces, including two edge pieces with chamfers and two middle pieces without chamfers; the photovoltaic module comprises a plurality of battery strings, each battery string comprises a plurality of first battery sections and a second battery section, each first battery section sequentially comprises an edge piece, a middle piece, a middle piece and an edge piece, the second battery section comprises two edge pieces and three middle pieces, or the second battery section comprises three edge pieces and two middle pieces. In the invention, the battery string is provided with the edge pieces and the middle pieces, so that waste caused by the fact that the middle pieces are not used can be reduced when the battery string is assembled. The number of the edge pieces and the number of the middle pieces in one battery string are only one, so that the using number of the edge pieces and the using number of the middle pieces in one battery string are balanced, and when the battery strings are assembled, the remaining number of the edge pieces and the remaining number of the middle pieces are small.

The invention relates to the technical field of bioengineering and fine chemical engineering, in particular to epoxide hydrolase, a gene, a carrier, engineering bacteria and application, and the amino acid sequence of the epoxide hydrolase is shown as SEQ ID NO.3. The epoxide hydrolase disclosed by the invention has the advantages that the epoxide hydrolase is Sphingopyis macrogoltabia EY-1, and the structural formula of the epoxide hydrolase is as shown in the specification. According to the invention, a recombinant plasmid pET-28a-smh containing an optimized gene and a genetic engineering bacterium E.coli / smh are constructed, the engineering bacterium is induced to over-express the recombinant epoxide hydrolase SmEH, and the recombinant epoxide hydrolase SmEH has catalytic activity. According to the present invention, the E.coli / smh whole cell is adopted to respectively catalyze the hydrolysis reaction of the (R)-styrene oxide (SO) and the (S)-styrene oxide (SO) so as to obtain the (R)-styrene oxide (SO) and the (S)-1, 2-phenyl glycol (1-Phenyl-1, 2-ethanediol, PED) with high optical purity, and the application of the E.coli / smh whole cell to respectively catalyze the hydrolysis reaction of the (R)-styrene oxide (SO) and the (S)-1, 2-phenyl glycol (1-Phenyl-1, 2-ethanediol, PED) is provided;

The invention discloses an escherichia coli recombinant bacterium for whole-cell catalytic synthesis of rebaudioside M as well as a construction method and application of the escherichia coli recombinant bacterium. The Escherichia coli recombinant bacterium for the whole-cell catalytic synthesis of the rebaudioside M, which is constructed by the invention, contains a glycosyl transferase UGT76G1 coding gene mutant, a glycosyl transferase UGT91C1 coding gene mutant, a sucrose synthase atSuS1 gene and a polyphosphoglucokinase cgPPGK gene; a glucose-1-phosphate uridine transferase galU gene, a nucleoside diphosphate kinase ndk gene and a nucleoside diphosphate kinase ndk gene are over-expressed; meanwhile, an encoding gene araA gene of L-arabinose isomerase and an encoding gene ushA gene of 5 '-nucleotidase / UDG hydrolase are knocked out. According to the recombinant escherichia coli constructed by the invention, Reb D is prevented from being directly used as a synthetic substrate, so that the raw materials are easier to obtain, and the conversion rate of the substrate and the synthetic rate and yield of rebaudioside M are remarkably improved.

The invention provides a method, device and system for evaluating the effect of intervening small molecules by tumors and a storage medium, and relates to the technical field of bioinformatics, the method comprises the following steps: obtaining a first transcriptome representation of a tumor cell population before target small molecule intervention and a second transcriptome representation of a normal tissue cell population corresponding to the tumors; inputting the first transcriptome representation and the condition vector into a prediction model to obtain a third transcriptome representation; calculating a first distance and a second distance; an intervention effect is evaluated based on a comparison of the first distance and the second distance. According to the method, the influence of the drug on the transcriptome of the whole cell population is efficiently predicted, and the distance between the transcriptome and the health state is quantitatively compared, so that a set of brand new standard for improving the evaluation target from the traditional'killing cell 'to'reversing disease state' is established, and an objective decision basis is provided for developing more accurate drugs.

The invention discloses recombinant escherichia coli for producing sucrose phosphorylase as well as a construction method and application of the recombinant escherichia coli, and belongs to the technical field of biological engineering. The sucrose phosphorylase is constructed, the amino acid sequence of the sucrose phosphorylase is shown as SEQ ID NO.11, a recombinant escherichia coli BdSP-L341V / L343F / V346P for expressing the sucrose phosphorylase is constructed, the sucrose phosphorylase expressed by recombinant bacteria or whole cells of the recombinant bacteria are used for carrying out catalytic reaction, and efficient production of ascorbyl glucoside can be realized. The invention also provides a method for producing ascorbic acid glucoside, the catalytic reaction is carried out at normal temperature and normal pressure, toxic and harmful organic solvents are not used, and the pollution to the environment is reduced; the whole catalytic process is a one-step reaction, has the advantages of high conversion rate, simple purification step and simple process, can better save the production cost, and has wide industrial application prospects.

The invention discloses a state-of-charge calibration method, electronic equipment, a storage medium and a vehicle. The state-of-charge calibration method comprises the following steps: determining that one part of a plurality of battery cells connected in series meets a target state-of-charge calibration condition and the other part of the plurality of battery cells does not meet the target state-of-charge calibration condition; on the basis of a first charge state calibration mode corresponding to the target charge state calibration condition, calibrating the charge state value of the battery cell meeting the target charge state calibration condition; and calibrating the state-of-charge value of each battery cell which does not meet the target state-of-charge calibration condition based on the second state-of-charge calibration mode. According to the method, the state-of-charge values of all the cells can be calibrated in different calibration modes, when the state-of-charge value of the whole cell is calibrated, the situation that the error of the state-of-charge value of the whole cell is increased due to the fact that part of the cells do not meet the target state-of-charge calibration condition is avoided, and the accuracy of estimating the remaining capacity of the whole cell is improved.

The invention relates to an engineering strain for producing (S)-higenamine, a method and application. The engineering strain is a recombinant pichia stipitis engineering strain containing coding genes of exogenous tyrosine hydroxylase mutants TyrHW13L and F309L, a dopa decarboxylase DODC and a higenamine synthase NCS. The recombinant pichia stipitis engineering strain is a recombinant pichia stipitis engineering strain containing coding genes of the exogenous tyrosine hydroxylase mutants TyrHW13L and F309L. According to the engineering strain, (S)-higenamine can be synthesized through a whole-cell catalytic reaction one-step method, exogenous coenzyme and exogenous reducing power are not needed, and the endogenous reducing power of the strain is utilized; the reaction path is short, products can be quickly accumulated in a short time, and the catalytic efficiency is high; the production cost is reduced.

The invention discloses an engineering strain for producing xylitol through one-step whole-cell transformation of corncob xylan. The engineering strain comprises a recombinant escherichia coli strain for catalyzing the corncob xylan to produce the xylitol through a one-step method and a recombinant saccharomyces cerevisiae strain for catalyzing the corncob xylan to produce the xylitol through a one-step method. The invention provides a novel method for producing xylitol through double-enzyme synergistic effect and catalysis of corncob xylan by using double-enzyme co-expression engineering strain whole cells for the first time, the corncob xylan is hydrolyzed by using engineering strain cells, enzyme purification is omitted, the step of purifying xylose is simplified, the loss of xylose is reduced, and the yield of xylitol is increased. Xylose produced by engineering strain cell enzymolysis is directly used for producing xylitol. Wherein the content of xylitol produced by conversion of the engineering strain 2 by taking 10g / L of corncob xylan as a substrate is 7.65 g / L, and the yield of xylitol is 76.50%.

The invention discloses a nitrilase mutant and application thereof in nicotinic acid biosynthesis, and belongs to the technical field of enzyme engineering. Through mutation design based on sequence fitness, the nitrilase mutant with improved nitrilase activity, thermal stability and soluble expression level is constructed, and the half-life period of the mutant D1F4 at 50 DEG C can reach 123.40 + / -5.94 min. The high-performance mutant obtained through construction is used for preparing nicotinic acid through whole-cell catalysis of 3-cyanopyridine, the final concentration of the product can be remarkably increased, and the final concentration of the product after 520 min of reaction can reach 2.21 M.

The present application relates to the technical field of fermentation engineering, and provides an engineered Escherichia coli for producing uroporphyrin as well as a construction method and application thereof.The present application uses Escherichia coli to express porphyrin synthase of thermophilic bacteria, performs whole-cell catalysis of 5-ALA to synthesize uroporphyrin under high-temperature conditions, and completely shields endogenous regulation; further, the Escherichia coli is wrapped by COF material to prevent the loss of enzymes in the cell recovery process; and a protein scaffold based on dockerin-cohesin is constructed to further improve the yield of uroporphyrin III.The present application creates a brand-new uroporphyrin high-temperature whole-cell catalysis process, realizes the efficient production of uroporphyrin, and provides a basis and idea for the industrial production of uroporphyrin.

The invention discloses a diketopiperazine heterodimer as well as a biosynthesis method and application thereof, and belongs to the technical field of bioengineering. The biosynthesis method of the diketopiperazine heterodimer comprises the following steps: S1, culturing a recombinant cell containing P450 dimerization enzyme or a mutant thereof, and performing induced expression to obtain a whole-cell culture; and S2, adding a cyclic dipeptide substrate containing tryptophan into the whole-cell culture, carrying out a catalytic reaction, and separating and purifying the obtained product to obtain the diketopiperazine heterodimer. According to the invention, the P450 dimerization enzyme or the mutant thereof is used for catalyzing the biosynthesis of the tryptophan-containing diketopiperazine into the diketopiperazine heterodimer with different regioselectivity / stereoselectivity, the biosynthesis method is green and environment-friendly, the efficiency is high, and the variety of the diketopiperazine heterodimer is greatly expanded.

The utility model relates to the field of culture solution concentration detection, in particular to an on-line protein concentration detection device for expanding a concentration range. The device comprises a first injection pump, a selector valve, a container assembly, a dilution and detection assembly and an air filter, the selector valve is provided with a public port and six selection ports uniformly distributed around the public port, and the public port is communicated with one of the six selection ports; a buffer ring is communicated between the first injection pump and the common port of the selection valve; the container assembly comprises a calibration standard liquid container, a first cleaning liquid container, a second cleaning liquid container and a culture container; the dilution and detection assembly comprises a three-way valve, a dilution assembly and a detection assembly respectively communicated with two ends of the three-way valve, and a sample reserving valve communicated with the output end of the detection assembly. According to the utility model, the detection concentration range is expanded to 6.5 orders of magnitude, the requirement of the protein measurement concentration range from extremely low concentration to extremely high concentration in the whole cell culture process is met, and the measurement accuracy and sensitivity are high.

Disclosed are systems and methods for producing renewable alcohols by fermentative processes using cell lysate or a combination of cell lysate and whole cells, as well as the cell lysate itself. The disclosure also provides processes and systems for converting renewable alcohols to transportation fuels, such as, gasoline, jet fuel, and diesel fuels.

The present application relates to a kind of whole cell catalytic synthesis N-acetyl-trans-4-hydroxyproline method, belong to biological catalysis technical field.The present application is constructed by mutating acyltransferase and heterologous expression gene engineering bacteria, successfully realized using whole cell catalytic synthesis N-acetyl-trans-4-hydroxyproline, avoids the problem of enzyme instability, reduces the separation cost of enzyme and product.On this basis, the present application improves the yield of N-acetyl-trans-4-hydroxyproline by optimizing catalytic time, cell addition amount, the type and addition amount of additive, and the yield reaches 239mg / L.The present application is a kind of N-acetyl-trans-4-hydroxyproline preparation method with lower cost, lower equipment demand, more suitable for industrial production.

The present invention provides an α-glucosidase mutant and its application in the synthesis of α-arbutin, including the following steps: using maltose and hydroquinone as substrates, and using the recombinant bacterium as a catalyst, carrying out a catalytic reaction under heating conditions to obtain the α-arbutin. The synthesis method of the present invention uses maltose and hydroquinone as reaction substrates and uses whole-cell catalysis to generate α-arbutin; at the same time, the sequence of the α-glucosidase mutant is integrated into the pRSEDuet-1 expression vector, transferred into Escherichia coli BL21(DE3) for expression, and the reaction system of the recombinant bacterium is optimized to determine the optimal catalytic conditions. Under the optimal conditions, the yield of α-arbutin is 24.8 g / L, and the yield can reach 95.6%, which is 1.2 times higher than that of the original strain, and has good application prospects.

This application discloses an embedded whole-cell biosensor, its fabrication method, and its application in machine learning-assisted mercury ion detection, belonging to the field of sensor materials and detection technology. This sensor encapsulates *E. coli* expressing green fluorescent protein (GFP) in a polyacrylamide / hyaluronic acid (PAAM / HA) hydrogel matrix, enabling highly selective and sensitive detection of mercury ions. It effectively eliminates interference from competing metal ions and can be completely regenerated through EDTA-2Na treatment, allowing for repeated use without performance loss. It maintains a stable fluorescence response for up to three weeks, possesses excellent biocompatibility and biodegradability, minimizing the risk of secondary contamination, and facilitates rapid on-site analysis.