379 results about "Propionibacterium" patented technology

The curative action of drugs, including herbal remedies, allopathic remedies, and periodontal remedies, is enhanced and accelerated by administering such drugs in combination or association with probiotics, especially those of genus Lactococcus, Lactobacillus, Pediococcus, Streptococcus, Propionibacterium, Brevibacterium, Penicillium, and Saccharomyces.

The present application relates to a probiotic composition comprising a probiotic bacteria. The composition is particularly useful as a food supplement for normalizing the gastro-intestinal flora. The probiotic bacteria present in the composition contain species of Propionibacterium, Lactobacillus, Bifidobacterium and Streptococcus. Also disclosed herein are methods of producing such composition and uses of the composition.

Methods are provided for improved immunization against coccidiosis and other bacterial, viral, or parasitic diseases in poultry. One method includes administering a solution of Propionibacterium acnes suspended in normal saline to a chick at age day one, following hatching. An anticoccidial vaccine and/or other vaccine is then administered to the chick such as by oral administration. Alternatively the method includes the steps of aseptically injecting Propionibacterium acnes in ovo at about day 18 in development, followed by post-hatching vaccination with an anticoccidial vaccine and/or other vaccine. Alternatively, either method can be utilized without the subsequent vaccination step for stimulating non-specific cell-mediated immune responses in poultry.

The invention discloses anti-deterioration cellar mud and a preparation method thereof. Based on 100 percent of yellow clay, the anti-deterioration cellar mud comprises the following components: 3 to 5 percent of yeast powder, 8 to 10 percent of double bottom dreg, 5 to 8 percent of mixed culture solution of caproic acid bacteria and liquid cellar mud, 2 to 3 percent of M type microbial fermentation liquor, 15 to 20 percent of peat, 5 to 8 percent of cellar mud, 10 to 12 percent of yellow water, 1 to 2 percent of propionibacterium liquid, 4 to 6 percent of impure alcohol, 2 to 3 percent soybean cake powder and 0.05 to 0.1 percent of K2HPO4. The cellar mud realizes the rational regulation of pH value, moisture, humus, nutrient substance, N, P, K and the like by the regulation of a formula and the ratio of the mixed culture solution of the M type microbe to the propionibacterium liquid to the caproic acid bacteria to the liquid cellar mud is well regulated, so that the pH value of the active and excellent cellar mud is always maintained between 5 and 7.

The invention relates to a method of producing propionic acid and propionate by microorganism fermentation, which is characterized by using microorganism fermentation technology to produce propionic acid; with propionibacterium, charging the nitrogen into basic fermentation culture medium which takes glycerin as a carbon source for anaerobic fermentation culture; movably adding the aqueous solution of calcium hydroxide, or the alkaline solution or the saline solution of sodium hydroxide and calcium carbonate with the ongoing of fermentation, adjusting the fermentation liquor and maintaining the pH value between 6.5 to 7.5; movably adding the aqueous solution of glycerin after the fermentation preformed for a period of time; the fermentation is finished when the content of glycerin stops increasing and is more than 35g/L; and then filtering the fermentation liquor through a pressure filter, getting the filtered solution, charging the filtered solution into a spray tower for spray drying, and the dried powder is the propionate product. The invention has the effects: the method can substitute the existing chemical synthesis to produce propionic acid, remarkably improving the safety of the propionic acid product, and is a green food additive; moreover, the problem of consumption of non-renewable resources (such as oil) can be solved without a secondary processing, the negative impact generated by chemical synthesis can be prevented, and the process flow and the cost can be saved; and the invention particularly opens up an industrialization stage of the propionic acid production by fermentation and the simultaneous production of the propionate.

The invention relates to application of a photosensitizer for treating acne. The photosensitizer is amino acid zinc phthalocyanine or a derivative thereof or organic or inorganic salt thereof which is used as the photosensitizer in photodynamic therapy for treating acne. The photosensitizer is of targeting and targeting aggressivity to target germs. Photons with special energy excite target molecules of the photosensitizer, the target molecules of the photosensitizer can transfer energy to oxygen molecules, and high energy oxygen molecules kill adjacent target germs: propionibacterium acnes, escherichia coli and staphylococcus aureus to treat acne. The photosensitizer provided by the invention used to treat acne is good in effect, free from antibiotics and hormone and tolerance, less in side effect, safe and nontoxic and economic and practical; can efficiently and quickly kill propionibacterium acnes, escherichia coli and staphylococcus aureus which induce acne to treat acne and restore skins without damaging normal cells; and is small in use level of effect medicines, and strong in effect of killing acne pathogenic bacteria.

The invention discloses a composite leavening agent combining xylanase with multiple strains and a method for fermenting a straw feed. The leavening agent consists of the following components in part by weight: 4 parts of xylanase, 2 parts of lactobacillus acidophilus, 2 parts of propionibacterium, 2 parts of saccharomyces cerevisiae, 3 parts of bacillus subtilis, 1 part of lactobacillus plantarum and 1 part of bacillus coagulans, wherein the xylanase can quickly degrade cell walls of straw plants and release nutrient substances; and under the synergistic effects of a plurality of microorganisms, fermentation speed is increased, fermentation period is shortened, fermentation activity is enhanced, protein conversion rate is increased and fermentation cost is lowered. The prepared fermented straw feed has low cellulose content, high protein content, high viable count, long retention period, soft and wet feel, strong acid incense, high palatability and high nutritional value. The problem of low resource utilization ratio of crops such as straws and the like is solved; simultaneously, environmental pollution is reduced; and the leavening agent and the method have high economic benefit and social benefit.

The invention discloses an acne-removing composition and a preparation method thereof. The acne-removing composition can be absorbed by skins faster compared with an emulsion preparation so as to take the function to the maximum extent. The function of comprehensively removing acnes is achieved by means of improving the moisturizing effect of the skins, adjusting the grease balance of the skins, inhibiting hypersecretion of sebum and inhibiting reproduction of propionibacterium acnes as well as by virtue of interaction of anti-inflammatory action and acne removal. The pH of the acne-removing composition is 4.5-5.5 (10% aqueous solution) which is faintly acid, and the acne-removing composition is mild in property and does not irritate the skins, and meanwhile, the effects of macromolecular salicylic acid and a composite plant acne-removing preparation are more obvious.

The invention discloses an acne-removing cream containing Chinese herbal medicine extracts and a preparation method thereof, and aims to provide the acne-removing cream having stronger inhibitory effect on propionibacterium acnes, having good acne-removing effect, being safe and gentle and having no irritation on human bodies. The technical key point comprises that the acne-removing cream is prepared from the following components by the mass percentage: 6-10% of an emulsifier, 2-3% of a coemulsifier, 10-14% of grease, 0.4-0.6% of an antioxidant, 1.6-2.4% of an absorption enhancer, 5-7.5% of an acne-removing composition, 1.6-2.4% of an emulsifier thickener, 8-12% of a moisturizer, 0.2-1% of a preservative, and the balance water. With the total mass of the acne-removing composition as 100%, the acne-removing composition comprises the components by the mass percentage: 6-10% of camphor, 6-10% of menthol, 12-20% of a peony root extract, 28-40% of a rheum palmatum root extract, 20-30% of a Chinese gall extract and 8-12% of a cortex phellodendri amurensis extract. The acne-removing cream belongs to the technical field of skin care products.

The invention discloses an apple enzyme and a preparation method. The preparation method comprises the following steps: juicing apples, uniformly mixing apple juice with pure water, adding glucose which accounts for 1-5% of the total weight of the obtained raw material mixture into the raw material mixture that the volume percentage of the apple juice is 5-20%, adjusting the pH value to 5.0-7.0, sterilizing, preparing mixed bacteria from the obtained raw material liquid and a pichia pastoris bacterial liquid and a lactobacillus plantarum bacterial liquid in a volume ratio of 1:(1-5), controlling the temperature to 25-35 DEG C, performing primary fermentation for 12-48 hours, controlling the temperature of the obtained primary fermentation solution to 25-35 DEG C by using an acetic bacterial liquid, performing secondary fermentation for 12-36 hours, and sterilizing, thereby obtaining a finished product of the apple enzyme. Compared with a finished product of natural fermentation, the finished product of the apple enzyme disclosed by the invention has a remarkable antibacterial effect on propionibacterium acnes, and the removal rate IC50 of the apple enzyme on DPPH is 0.43%.

The invention discloses a polypeptide composition used for removing an acne and repairing the skin, the polypeptide composition comprises the following components by weight: 0.5-5 parts of antibacterial peptide, 0.01-3 parts of anti-inflammatory cytokine, 0.01-2 parts of a cell growth factor, 0-60 parts of a stabilizer, 0-40 parts of a humectant, 0-15 parts of an antioxidant, 0-30 parts of a solubilizer, and 800-950 parts of water. A product can effectively inhibit the propionibacterium acnes, the acne is fundamentally treated and prevented, the inflammatory reaction of the skin is effectivelyeliminated, the injured cell is repaired, the acne pit and the acne mark are effectively removed, and the product is non-irritant, is suitable for people suffering from skin allergy, and has the fundamental effects of removing the acnes and repairing the skin.

The invention discloses a fibrous bed reactor and the equipment and process for extracting separation and coupling production of propionic acid. The invention utilizes an immobilized fibrous bed reactor unit, a membrane separation unit, an extraction and anti-extraction unit and an elution and condensation unit to realize the efficient production of propionic acid. The device has strong feasibility, simple operation, easy realization of automation, immobilized materials, long using life of the membrane and the matching equipment, convenient maintenance and good application prospect. Through extracting separation and coupling production technology, the separation of propionic acid is realized. The invention radically eliminates the feedback inhibition of propionic acid, implements the high density fermentation of propionibacterium and greatly improves the production efficiency of propionic acid through the fermentation method. The extracted water phase is returned to the reactor for recycle after elution and condensation by an elution device, which eliminates the harm of extractant for propionibacterium from the root.

The invention discloses a botanical medicine composition with an acne removing effect and application. The botanical medicine composition is prepared from radix salviae miltiorrhizae, pomegranate bark, spreading hedyotis herb, rhizoma curcumae and licorice roots. The botanical materials are smashed, screened and mixed, the mixture is added into an extraction solvent and then soaked, room-temperature extraction is carried out with a high-pressure homogenization extraction machine, extraction liquor is subjected to centrifugal separation and then concentrated with a membrane, concentrated liquor is adsorbed with macroporous resin and then eluted repeatedly with ethanol, elution liquor is collected, butanediol is added for dissolution after membrane concentration, filtering is carried out, and the botanical medicine composition is obtained, wherein water or ethanol or an ethanol aqueous solution serves as the extraction solvent. The botanical medicine composition can inhibit secretion of skin grease, effectively inhibit propionibacterium acnes and achieve a good acne removing effect, and can enhance skin immunity, improve skin nature and make skin permeable, clean, smooth and fine when used for a long term.

The invention provides a preparation method of a probiotic and aloe mask, which is characterized by preparing a fermentation stock solution from aloe vera raw juice, glucose and water according to certain proportions and adding probiotics for performing co-fermentation. By setting different temperature gradients and pH gradients, after viable count, optimal fermentation conditions are determined; an aloe and probiotic fermentation solution is adopted to carry out an antibacterial test so as to determine an antibacterial effect, such as propionibacterium acnes; and meanwhile, by determining an antioxidant index, the antioxidant ability of the aloe and probiotic fermentation solution is determined, such as determination of DPPH radical scavenging ability, determination of iron ion chelating ability and determination of total reducing force. According to the product, the aloe and probiotic fermentation solution is mixed with cosmetic auxiliary materials in proportion so as to be processed into a pasty mask, and after sensory index testing, skin irritation testing and trial effect testing, the safety and functionality of the product are determined, so that the aims of skin protection and health care are achieved.

The invention discloses a method for preparing antibacterial metabolin of Propionibacterium, the Propionibacterium (Propionibacterium.thoenii) is stored in American Type Culture Collection (ATCC) with the storing number of ATCC 4874. In the invention, the Propionibacterium is activated and fermented by microorganism fermentation technology to obtain the antibacterial metabolin containing the Propionibacterium. The antibacterial metabolin of Propionibacterium can be used in food anticorrosion and fresh-keeping, disinfector, anti-infection medicine, animal and plant disease control and the medicine field for inhibiting gram-negative bacteria, mould and microzyme. The method for preparing the antibacterial metabolin is convenient in operation, is low in production cost and has wide application prospect.

The invention provides Saccharomycopsis fibuligera with the collection number of CCTCC M 2019324. Tests prove that the Saccharomycopsis fibuligera CCTCC M2019324 has DPPP free radical scavenging rateof an extracellular supernatant is 81.9% and a tyrosinase inhibition rate of 68.1%. The extracellular supernatant of Saccharomycopsis fibuligera CCTCC M2019324 is fermented with a medicinal plant as asubstrate, and a fermentation liquid of the extracellular supernatant has a better acne propionibacterium inhibition effect by detection. Meanwhile, the medicinal plant fermentation liquid can also be applied to preparation of cosmetics, and a prepared product of the plant fermentation liquid containing the e Saccharomycopsis fibuligera CCTCC M2019324 can effectively scavenge free radicals, promote skin renewal and reduce synthesis of melanin and has very good application prospects.

The invention discloses acne-removing cream. The acne-removing cream is prepared from hexamidine diisethionate, a hydroxypropyl-beta-cyclodextrin inclusion compound of azelaic acid, a hydroxypropyl-beta-cyclodextrin inclusion compound of salicylic acid and oat alkaloid derivatives. Permeation and stripping effects of salicylic acid in the hydroxypropyl-beta-cyclodextrin inclusion compound of salicylic acid is beneficial to permeation of other components into the skin basal layer, and the hexamidine diisethionate, a hydroxypropyl-beta-cyclodextrin inclusion compound of azelaic acid are enabledto more effectively kill propionibacterium acnes to fundamentally treat acnes, and great acne removing effects are achieved. By the hydroxypropyl-beta-cyclodextrin inclusion compound of azelaic acid,oil secretion and chromatosis can be evidently reduced while antibacterial acne-removing effects are achieved, water-oil balance is effectively regulated, and acne reduction and acne mark fading are realized; by the oat alkaloid derivatives, skin irritation symptoms such as allergy and stabbing pain caused by other components can be alleviated, and swelling, itching and pains of acne affected parts can be alleviated.

The invention provides a polypeptide having the molecular weight of 3321.6 Da and having an amino acid sequence as shown in SEQ ID NO.1. The invention also provides six derivatives of the polypeptide. The polypeptide and the derivatives thereof provided by the invention have efficient broad-spectrum bacteriostatic effect on gram positive bacteria and gram negative bacteria (especially having good killing effect on anti-clindamycin anaerobic propionibacterium acnes), fungi and yeasts, and have high activity (the concentration for killing the propionibacterium acnes in vitro can be only 0.125 mg/L). The polypeptide and the derivatives thereof can be applied in preparation of microbial bacterial and fungal infection-controlling and anti-inflammatory products, wherein the products include externally-applied disinfectants, anti-bacterial agents, wound-protection or infection-prevention dressings, acne treating drugs, skin care products, cosmetics and the like. The polypeptide is designed and synthesized based on a tryptophan bromide-containing fragment of a marine biological sea urchin, has stronger bacteria inhibiting or killing and anti-inflammatory effects and better stability, and can be used as an antibiotic substitute.

The invention discloses a traditional Chinese medicine composition for removing acne and an application thereof. The pharmaceutical composition comprises the following components: an extract product of Centella asiatica, an extract product of scutellaria roots, vitamin E, clove oil, an extract product of licorice, an extract product of flavescent sophora, hydrolyzed pearl, and borneol, and the materials and a proper amount of matrix are used for preparing a preparation for external use. The traditional Chinese medicine composition has efficacies for clearing heat and draining dampness, detoxifying, and dispersing the swelling; the composition has multiple efficacies for better inhibiting growth of Propionibacterium acnes without generation of drug resistance, resisting hyperplasia of scar, resisting chromatosis, resisting inflammation, easing pain, preserving moisture, adjusting balance of oil and fat, and the like; the product has obvious anti-acne effects, and can be used for thoroughly eradicating acne without recurrence after healing; the product does not have toxic and side-effects, and acne removal does not leave marks. Total effective rate of the traditional Chinese medicine composition for treating acne in four weeks is 100%, and acne of healed patients does not recur. After acne is treated, total effective rate for resisting chromatosis reaches 85.71%, the curative effects are better than the effects of Tretinoin cream, and obvious adverse reactions are not observed.