66 results about "Propionibacterium" patented technology

The application discloses a bacteriostatic and acne-removing microemulsion containing plant extracts and a preparation method thereof. The bacteriostatic and acne-removing microemulsion contains a microemulsion loaded with quercetin alone, a microemulsion loaded with sasanquasapernin alone, and a microemulsion loaded with quercetin and sasanquasapernin simultaneously. The bacteriostatic and acne-removing microemulsion containing plant extracts prepared by the application not only solves the problems of stickiness of camellia oil and poor solubility of quercetin, but also has a synergistic inhibitory effect on propionibacterium acnes, achieves the same high-efficiency bacteriostatic effect with a lower drug concentration, saves drug cost, reduces drug irritation, and expands the application of quercetin and sasanquasapernin in the cosmetic and pharmaceutical fields due to the rich pharmacological effect of the plant extracts, low side effect and safety. The microemulsion system prepared by the application is clear, transparent, uniform and stable, the preparation process is simple, the required energy is low, the cost is low, and the system is beneficial to commercial production.

The invention relates to composite cosmetic nanoparticles with an acne removing effect and a preparation method of the composite cosmetic nanoparticles, and belongs to the technical field of functional skin care products and nano entrapment. According to the nano-particles, low-molecular chitosan is used as a main carrier, and natural multifunctional acne-removing components including a white willow bark extract, a tea catechin substance, a pomegranate peel extract and a hovenia dulcis thunb fruit extract are jointly embedded, so that a stable nano-particle structure with the particle size of 50-200 nm is formed; and sodium hyaluronate is used for surface modification so as to enhance skin adhesiveness and permeability. The multi-target synergistic active component can effectively inhibit the growth of propionibacterium acnes, relieve inflammatory response, regulate sebum secretion and enhance the antioxidant repair capacity of skin. The nano-particles prepared by the invention are concentrated in particle size distribution, high in entrapment rate and good in biocompatibility, are suitable for essence, spray, mask type and gel type acne-removing skin care products, and show excellent product transformation potential.

The invention belongs to the technical field of cosmetic raw materials, and particularly relates to a plant composition with oil control and acne removal effects and a preparation method and application thereof. The invention provides an oil-controlling and acne-removing plant composition. The oil-controlling and acne-removing plant composition is prepared from a rheum palmatum extract, a scutellaria baicalensis extract, a radix sophorae flavescentis extract, a safflower extract, a campsis grandiflora extract, a chrysanthemum extract, a cockscomb extract and a rose extract in a specific proportion. Through synergistic compatibility of eight plants, the plant composition can effectively inhibit grease synthesis, inflammatory factor expression and propionibacterium acnes growth, so that good oil control and acne removal effects are achieved.

This invention provides ozenafloxacin nanocrystalline particles, its preparation method, and its uses. The preparation method includes the following steps: dissolving ozenafloxacin raw material in an acidic aqueous solution, then adding a stabilizer, stirring, and adjusting the pH to 4-6; wherein the stabilizer is a siloxane and / or a cellulose derivative. The preparation method provided by this invention can obtain ozenafloxacin nanocrystalline particles with uniform and stable particle size distribution. Further research by the inventors has found that formulations containing these ozenafloxacin nanocrystalline particles can increase the amount of drug retained in the skin, thereby increasing the local drug concentration, achieving effective killing of Propionibacterium acnes, and preventing the development of drug resistance.

The application belongs to the technical field of microorganisms and particularly relates to a Bifidobacterium adolescentis beneficial to skin, a derivative of the Bifidobacterium adolescentis and application of the Bifidobacterium adolescentis and the derivative. The derivative of the Bifidobacterium adolescentis has significant antioxidant and anti-inflammatory effects, can inhibit pathogenic bacteria of acne and other common skin infections such as Propionibacterium acnes, Staphylococcus aureus and Malassezia furfur, can eliminate pathogenic bacteria, can inhibit skin lipid accumulation, can avoid follicle blockage, and can improve skin problems in multiple directions, especially acne skin problems.

The invention belongs to the technical field of medical experimental animal models, and particularly relates to a construction method of a rabbit ear acne model for studying acne pathogenesis and drug curative effect. A healthy New Zealand large-ear white rabbit is selected, a testosterone solution is subcutaneously injected to induce endocrine disorder, oleic acid is smeared at an opening of an external auditory canal on the inner side of an ear, dermatobacterium acnes suspension is subcutaneously injected, and whether acne-like lesions such as erythema, papule and comedo appear on the skin of the ear or not is observed. The method is easy and convenient to operate, low in cost, good in repeatability, capable of simulating the pathological process of human acne and suitable for acne pathogenesis research and drug screening.

This invention discloses an anti-acne Propionibacterium acnes compound essential oil, its preparation method, and its application. The anti-acne Propionibacterium acnes compound essential oil is composed of the following components in the indicated mass percentages: 22%~28% helichrysum essential oil, 22%~28% sweet basil essential oil, 22%~28% eucalyptol rosemary essential oil, and 22%~28% true lavender essential oil. This invention achieves highly effective antibacterial activity against Propionibacterium acnes through a precise selection of four plant essential oils and a scientifically formulated compound. It also possesses multiple benefits including anti-inflammatory properties, promotion of skin cell regeneration, and a natural fragrance. Furthermore, the product is natural, safe, and has no side effects, is suitable for various skin types, and can be directly applied to the preparation of various acne-removing skincare products.

The invention relates to the field of cosmetic raw material development and cosmetics, in particular to a cosmetic composition containing oxidoreductase as well as a preparation method and application of the cosmetic composition. The composition is prepared from the following components in parts by weight: 1.5 to 3.5 parts of oxidoreductase, 0.3 to 2.5 parts of aloe barbadensis leaf extract, 0.5 to 3 parts of pimpinella diversifolia fruit extract, 0.6 to 4 parts of lysozyme, 0.8 to 2.5 parts of papain, 0.5 to 1.8 parts of lysozyme, 0.5 to 3.5 parts of pansy extract and 0.5 to 3 parts of amur corktree bark extract. The composition prepared by the invention has excellent efficiency of inhibiting propionibacterium acnes and elastinase, and has the effects of removing acnes, tightening skin and resisting aging; the composition uses active components for moisturizing, anti-inflammation, sterilization and the like, and also has good effects of sensitive repair and skin micro-ecology regulation.

The invention relates to a composition, a mask and application thereof. The invention particularly relates to a composition which comprises a functional material and a spinning matrix, the functional material comprises a black willow bark extract and a hydrogen storage material, and relative to the total mass of the composition, the mass fraction of the black willow bark extract is 0.130%-0.210%, and the mass fraction of the hydrogen storage material is 0.05%-0.20%; the mass fraction of the spinning matrix is 8%-20%. The composition can control grease secretion, improve facial grease accumulation, reduce the number of acnes of a subject, fade acne marks, inhibit growth of propionibacterium acnes and improve acnes.

The invention discloses a composition of antibacterial peptide and a plant extract. The composition is prepared from epsilon-polylysine, ginseng root water and raspberry fruit water. Compared with a single component, the composition of the antibacterial peptide and the plant extract has the advantages that the antibacterial and bactericidal effects are obviously improved, and the composition has an obvious antibacterial effect on propionibacterium and malassezia, and has an easy-to-comb function when being used in hair washing products. The composition has good effects of resisting bacteria, removing acnes and removing dandruff, is soft and easy to comb, and has storage stability.

This invention discloses a method for enhancing the antibacterial activity of Propionibacterium freudenreichii subsp. shermanii metabolites by adding a surfactant to a culture medium. The method uses Propionibacterium freudenreichii subsp. shermanii NBRC No. 12426 as the experimental strain and Pichia anomala SICC 2.894 as the indicator strain. A surfactant is added to the fermentation medium to enhance the antibacterial activity of the metabolites of Propionibacterium freudenreichii subsp. shermanii against the indicator strain Pichia anomala. Based on the culture conditions and method of Propionibacterium freudenreichii subsp. shermanii in this invention, the optimal concentration of the surfactant Tween-80 is selected as 0.3%, which significantly enhances the antibacterial activity of the metabolites of Propionibacterium freudenreichii subsp. shermanii.

This invention relates to the field of antibacterial product technology, specifically to a plant composition with antibacterial effects, its preparation method, and its application. The plant composition, by weight, comprises the following raw material components: 2-6 parts of Lophatherum gracile, 2-5 parts of Rhodomyrtus tomentosa, 1-5 parts of Phellodendron amurense, and 2-8 parts of Podocarpus macrophyllus. The plant composition of this invention has the advantages of good efficacy and no side effects in inhibiting Staphylococcus aureus, Escherichia coli, Candida albicans, Malassezia furfur, and Propionibacterium acnes.

The invention belongs to the technical field of medicines and the field of acne treatment, and particularly relates to application of amorphous salt in preparation of a medicine for treating acne and the medicine. The amorphous salt is prepared by reacting ursodesoxycholic acid and matrine, preferably, the ursodesoxycholic acid and the matrine are dissolved in a proper solvent according to the molar ratio of 1: (0.5-2), then the solvent is rapidly removed, and remaining solids are further dried and crushed to obtain the ursodesoxycholic acid / matrine amorphous salt. The solubility and the percutaneous penetration rate of the propionibacterium acnes are remarkably improved, the propionibacterium acnes can be inhibited, the expression of IL-1beta, IL-6, IL-8 and TNF-alpha can be reduced, P65 protein nucleus translocation can be inhibited, and an efficient and safe new scheme is provided for acne treatment.

The present application relates to the field of agricultural biotechnology and plant disease control technology, and specifically discloses a composition for preventing and controlling rape root rot, a preparation method and application thereof. The composition is prepared by fermentation of the following components in parts by weight: tea seed cake 10-30 parts, oyster shell powder 20-30 parts, waste molasses 10-20 parts, brewer's yeast powder 1-5 parts, xylo-oligosaccharide industrial residue 1-3 parts, and a composite fermentation agent containing lactobacillus plantarum, bacillus coagulans and propionic acid-producing propionibacterium 1-3 parts. The fermentation process adopts a two-stage pH control timing inoculation process: in the first stage, brewer's yeast is inoculated; in the second stage, the composite fermentation agent is inoculated under acidic conditions, and through synergistic metabolism, the sugar source is efficiently converted into a short-chain fatty acid composite system mainly composed of lactic acid and propionic acid. The prevention and control effect on rape root rot is significantly improved, and the composition also has the functions of improving soil and promoting crop growth, and has the advantages of high efficiency, environmental protection and multi-effect combination.

The invention provides a phenolic amide derivative and application thereof, relates to application of a compound and a composition thereof in preparation of cosmetics or medicines for preventing, treating and relieving acne, and provides a medicine or cosmetic composition which comprises a therapeutically effective amount of compound, or at least one of a racemate, a stereoisomer, a geometric isomer, a tautomer, an isotope label, a hydrate, a solvate, a polymorphic substance, a metabolite, a prodrug and pharmaceutically acceptable salt or ester. The present invention provides the prevention, treatment or alleviation of skin problems associated with Propionibacterium acnes, including comedo, acne, folliculitis; according to the phenolic amide derivative with a novel structure, the structural type of the phenolic amide compound is enriched, the activity of inhibiting propionibacterium acnes is increased on the basis of retaining the anti-inflammatory activity of the phenolic amide compound, and the aim of treating both symptoms and root causes of skin problems related to the propionibacterium acnes is achieved.

The present invention relates to a culture medium composition containing sugars and amino acids derived from human skin, microorganisms of the genera Staphylococcus, Streptococcus, Cutibacterium, Corynebacterium, and Rothia, and a cosmetic composition containing their lysate or culture medium. The mixed culture medium of human skin-derived microorganisms contained in the cosmetic composition is cultured in the culture medium composition and exhibits a significantly superior skin-improving effect compared to each of the individual culture mediums.

The present invention relates to a combination comprising an extract of Myrtus communis and an extract of Tripterygium wilfordii, in particular for the treatment of inflammation caused by Propionibacterium acnes and for the treatment of acneic skin.

The present application provides a kind of epidermis repair gel dressings and its preparation method, it is related to dressing field.The epidermis repair gel dressings includes repair liquid, 100 parts of the repair liquid includes composite sodium hyaluronate 0.1-0.3 parts, carbomer 0.02-0.06 parts, glycerol 1-4 parts, pentanediol 1-3 parts, composite acid 0.05-0.1 parts, composite peptide 0.01-0.03 parts and the balance purified water by weight parts;The composite sodium hyaluronate is composed of sodium hyaluronate, hydrolyzed sodium hyaluronate and sodium hyaluronate crosslinked polymer;The composite acid is prepared from ursolic acid, hydroxyl asiatic acid, lipoic acid;The composite peptide is prepared from thirteen peptides and palmitoyl pentapeptide-3.The epidermis repair gel dressing of the present application has better repair effect on acne lesions, not only inhibits active propionibacterium acnes, but also can reduce the expression of inflammatory factors, can deeply repair damaged basal structure, maximumly reduce the degree of acne lesions, and restore to close normal skin.

The application discloses a targeted acne-removing composition suitable for sensitive skin and a preparation method and application thereof. The targeted acne-removing composition comprises lysozyme, a first extract and a second extract in a mass ratio of 1:(0.1-10):(0.1-5). The first extract comprises guaiacum extract and myrtle fruit extract. The second extract comprises sophora flavescens extract and angelica sinensis extract. The targeted acne-removing composition containing lysozyme, the first extract and the second extract in specific mass ratios can inhibit the activity of propionibacterium acnes, down-regulate the expression of Toll-like receptor 2 (TLR2) and Toll-like receptor 4 (TLR4), and achieve efficient acne removal, and has the mild property and can be suitable for sensitive skin.

The present application belongs to the technical field of skin care products, and particularly relates to a composition for treating acne and a preparation method thereof. The composition comprises the following components: 1-2% of a lactic acid bacillus fermentation product, and 5-8% of a plant extraction component; the plant extraction component is composed of a Pinus Nigra Leaf Extract, a Osmorhiza Aristata Root Extract, and a Echinacea Purpurea Extract. The mass ratio of the Pinus Nigra Leaf Extract, the Osmorhiza Aristata Root Extract, and the Echinacea Purpurea Extract is 1:(1-3):(5-10). The Osmorhiza Aristata Root Extract, the Pinus Nigra Leaf Extract, and the Echinacea Purpurea Extract have a certain synergistic effect in the composition formula, and therefore, the acne treatment composition provided by the present application can effectively inhibit the growth of Propionibacterium acnes, reduce the occurrence of skin inflammation, effectively treat acne, and has a good market application prospect.

The invention discloses torreya grandis seed oil and application thereof, and belongs to the field of plant extracts. The torreya grandis seed oil is prepared by adopting a supercritical CO2 extraction method, the expression level of a macrophage RAW264.7 inflammatory factor IL-6 caused by LPS can be remarkably reduced, and the torreya grandis seed oil has a good anti-inflammatory effect. The traditional Chinese medicine composition disclosed by the invention can effectively inhibit the growth of propionibacterium acnes, also can effectively regulate the skin flora balance of people with acnes, has no obvious influence on the growth of staphylococcus epidermidis while remarkably inhibiting the growth of propionibacterium acnes, is an excellent acne-removing cosmetic raw material, and has a wide application prospect in the development of nursing products for people with acnes.

The present application relates to the technical field of biological medicine, in particular to a traditional Chinese medicine composition for improving acne and application, which is made of medicinal raw materials including a first component, a second component and a third component, wherein the first component includes 25-35 parts of white yam, 25-35 parts of hawthorn, 25-35 parts of salvia miltiorrhiza, 13-17 parts of gardenia, and 8-12 parts of white peony; the second component includes 25-35 parts of nacre, 13-17 parts of tribulus terrestris, and 8-12 parts of safflower; and the third component includes 13-17 parts of raw rhubarb, 8-12 parts of gallnut, 8-12 parts of turmeric, 3-7 parts of ganoderma lucidum, and 3-7 parts of winter nightshade. The traditional Chinese medicine composition has the dual effects of inhibiting propionibacterium acnes and anti-inflammation, and especially, the whole composition shows a significant synergistic effect in anti-inflammation in vivo, which is better than each component alone or any two components in combination. The composition can be prepared into oral or external medicines for preventing, relieving and treating various types of acne, and can also improve post-acne pigmentation and post-acne scarring, and has a good application prospect with a significant clinical effect and high safety.

The invention belongs to the technical field of aquatic product pathogen gene editing, and particularly relates to a target gene knockout method for photobacterium damsela subsp. Piscicida (PDP), which is characterized in that the target gene knockout method for the photobacterium damsela subsp. Piscicida (PDP) is disclosed. The method comprises the following steps: obtaining a DNA fusion fragment with deletion of a target region fragment of a target gene (MshL) by adopting overlapping PCR (Polymerase Chain Reaction), constructing a recombinant vector by using the obtained DNA fusion fragment, and obtaining the target gene knocked-out photobacterium damnieri subspecies fish by taking escherichia coli S17-1 lambda pir as a donor bacterium of a recombinant plasmid and PDP as a recipient bacterium. Specific knockout of the PDP specific gene is achieved for the first time, the knockout efficiency is high (the single-exchange positive rate is 29.2%, and the double-exchange positive rate is 30.8%), the cost is low, and the method is suitable for construction of a PDP specific gene deleted strain and functional research of the specific gene.

The invention provides a lightyellow sophora root fermentation extract, a preparation method and application in an anti-hair loss product, the preparation method comprises the following steps: step 1, soaking lightyellow sophora root in water, cutting up, then inoculating a paecilomyces cicadae seed solution for fermentation culture, and after paecilomyces cicadae mycelium overgrows the lightyellow sophora root, drying to obtain a paecilomyces cicadae-lightyellow sophora root bidirectional fermentation product; and 2, crushing the paecilomyces cicadae-radix sophorae flavescentis bidirectional fermentation product, extracting by adopting a bifidobacterium liquid fermentation extraction method, or a hot water extraction method or an absolute ethyl alcohol extraction method, and drying to obtain the radix sophorae flavescentis fermentation extract. The sophora flavescens fermentation extract prepared by the method has good II type 5 alpha reductase inhibition capability, and the ethanol extract CH-KS-A of the penicillium-sophora flavescens bidirectional fermentation product particularly has broad-spectrum antibacterial activity on staphylococcus aureus, propionibacterium acnes and malassezia furfur, and has good anti-inflammatory and antioxidant activity; the composition can be used in anti-hair loss products.

The invention belongs to the field of bioengineering and solid waste resource utilization, and relates to a kitchen waste pretreatment method for propionic acid fermentation, a special microbial inoculum and a fermentation assistant.The kitchen waste pretreatment method for propionic acid fermentation comprises the steps that kitchen waste is crushed into particles of 2-5 mm, the particles are mixed with water according to the solid content of 40%-80%, the mixture is heated at the temperature of 50-80 DEG C and then filtered, and a mixture is obtained; filtering to obtain filtrate; performing oil-water separation on the filtrate to obtain a water phase and an oil phase; adding 1%-10% (v / v) of the obtained oil phase back into the water phase, and emulsifying at the rotating speed of 800-1000rpm; sequentially carrying out enzymolysis treatment of lipase hydrolysis, alpha-high-temperature amylase hydrolysis and glucoamylase hydrolysis on the emulsified treatment liquid; after impurity adsorption, enzyme deactivation is performed to obtain the propionibacterium fermentation substrate. The strain can be used for efficient substrate conversion, has convenient microbial inoculum application, optimizes the fermentation process, improves economic and environmental benefits, and has industrial application potential.

This invention discloses a method for de novo design of antifungal peptides, the obtained peptides, and their applications. The method includes: S1 peptide library retrieval and amino acid preference analysis; S2 peptide design based on multiple parameters; S3 AI high-throughput screening of the peptide library to obtain candidate antifungal peptides. The amino acid sequences of the mentioned antifungal peptides are also disclosed in this invention as shown in SEQ ID Nos: 1 to 3. The antifungal peptides developed by this invention can specifically target and kill fungi such as Malassezia furfur without inhibiting the growth of other beneficial bacteria such as Propionibacterium acnes, thereby effectively regulating the skin surface microecological balance and maintaining skin health; solving the problem that current antimicrobial peptides, due to their broad-spectrum antibacterial properties, can disrupt the skin microecological balance and cause more serious skin diseases.

This application provides the use of an antimicrobial peptide in the preparation of skin care products. The antimicrobial peptide of this application can effectively inhibit *Propionibacterium acnes* and *Malassezia*, thereby preventing skin problems such as acne, skin inflammation, and dandruff caused by the overgrowth of these two bacteria. Therefore, the antimicrobial peptide of this application has skin care uses in inhibiting acne, relieving skin inflammation such as folliculitis, pityriasis versicolor, and seborrheic dermatitis, and in inhibiting dandruff, and has significant practical value.

The invention discloses an antibacterial peptide for inhibiting staphylococcus aureus and propionibacterium acnes as well as a preparation method and application of the antibacterial peptide. The antibacterial peptide comprises any one or more than two peptides in the following amino acid sequences SEQ ID NO.1-8. The antibacterial peptide shows efficient and balanced inhibitory activity on staphylococcus aureus and propionibacterium acnes, due to the characteristic, a single antibacterial peptide can adapt to multi-scene infection treatment, and multiple antibacterial peptides do not need to be matched for different infection scenes, so that the suitability and practicability of clinical application are remarkably improved.

The invention belongs to the technical field of medicines and cosmetics, and relates to a composition and application thereof in preparation of oil-control and acne-removal products for sensitive skin. The composition is prepared from the following active ingredients in parts by weight: 0.01 to 0.5 part of picea norvegensis leaf extract, 0.01 to 0.5 part of PCA zinc and 0.001 to 0.05 part of bacillus fermentation product. The composition disclosed by the invention is simple in component type, all the components are in mutual synergistic interaction, and propionibacterium acnes are effectively inhibited, so that an excellent acne removing effect is realized.

This invention relates to the field of biomedical technology, specifically to a topical composition for improving acne and its application. The topical composition is made from pharmaceutical raw materials including a first component, a second component, and a third component. The first component comprises 25-35 parts of *Ampelopsis japonica*, 25-35 parts of hawthorn, 25-35 parts of *Salvia miltiorrhiza*, 13-17 parts of gardenia, and 8-12 parts of *Bletilla striata*. The second component comprises 25-35 parts of mother-of-pearl, 13-17 parts of tribulus terrestris, and 8-12 parts of safflower. The third component comprises 13-17 parts of raw rhubarb, 8-12 parts of gallnut, 8-12 parts of turmeric, 3-7 parts of *Ganoderma lucidum*, and 3-7 parts of asparagus. This invention also provides a topical composition consisting of the first, second, or third component alone. The topical composition of this invention has dual effects of inhibiting *Propionibacterium acnes* and anti-inflammatory properties, and can be further prepared into cosmetics or pharmaceuticals for the prevention, relief, and treatment of various types of acne and the improvement of acne scars. Clinical trials have shown significant effects, high safety, and promising application prospects.