383 results about "Propionibacterium" patented technology

The curative action of drugs, including herbal remedies, allopathic remedies, and periodontal remedies, is enhanced and accelerated by administering such drugs in combination or association with probiotics, especially those of genus Lactococcus, Lactobacillus, Pediococcus, Streptococcus, Propionibacterium, Brevibacterium, Penicillium, and Saccharomyces.

The present invention relates to probiotic Propionibacterium strains and their use in the preparation of probiotic supplements and foods. The invention relates to the provision of Vitamin B12, propionic acid, folacin and bacteriocins by probiotic strains, stimulation of bifidobacteria growth, production of favourable effects on the lipid metabolism and on the immune system of hosts through immunostimulation, immunomodulation or use of a probiotic strain as an adjuvant, reduction of homocysteine and β glucuronidase and the prevention, treatment or amelioration of conditions associated with a need for these activities. The probiotic bacteria of the invention can be used in humans or other animals. In at least some applications, the bacteria can be used dead and parts rather than whole cells may be used. The present invention also relates to the preparation of vaccines for use in protecting patients from infectious diseases, in particular tuberculosis.

The present application relates to a probiotic composition comprising a probiotic bacteria. The composition is particularly useful as a food supplement for normalizing the gastro-intestinal flora. The probiotic bacteria present in the composition contain species of Propionibacterium, Lactobacillus, Bifidobacterium and Streptococcus. Also disclosed herein are methods of producing such composition and uses of the composition.

Methods are provided for improved immunization against coccidiosis and other bacterial, viral, or parasitic diseases in poultry. One method includes administering a solution of Propionibacterium acnes suspended in normal saline to a chick at age day one, following hatching. An anticoccidial vaccine and / or other vaccine is then administered to the chick such as by oral administration. Alternatively the method includes the steps of aseptically injecting Propionibacterium acnes in ovo at about day 18 in development, followed by post-hatching vaccination with an anticoccidial vaccine and / or other vaccine. Alternatively, either method can be utilized without the subsequent vaccination step for stimulating non-specific cell-mediated immune responses in poultry.

The invention discloses anti-deterioration cellar mud and a preparation method thereof. Based on 100 percent of yellow clay, the anti-deterioration cellar mud comprises the following components: 3 to 5 percent of yeast powder, 8 to 10 percent of double bottom dreg, 5 to 8 percent of mixed culture solution of caproic acid bacteria and liquid cellar mud, 2 to 3 percent of M type microbial fermentation liquor, 15 to 20 percent of peat, 5 to 8 percent of cellar mud, 10 to 12 percent of yellow water, 1 to 2 percent of propionibacterium liquid, 4 to 6 percent of impure alcohol, 2 to 3 percent soybean cake powder and 0.05 to 0.1 percent of K2HPO4. The cellar mud realizes the rational regulation of pH value, moisture, humus, nutrient substance, N, P, K and the like by the regulation of a formula and the ratio of the mixed culture solution of the M type microbe to the propionibacterium liquid to the caproic acid bacteria to the liquid cellar mud is well regulated, so that the pH value of the active and excellent cellar mud is always maintained between 5 and 7.

The invention relates to a method of producing propionic acid and propionate by microorganism fermentation, which is characterized by using microorganism fermentation technology to produce propionic acid; with propionibacterium, charging the nitrogen into basic fermentation culture medium which takes glycerin as a carbon source for anaerobic fermentation culture; movably adding the aqueous solution of calcium hydroxide, or the alkaline solution or the saline solution of sodium hydroxide and calcium carbonate with the ongoing of fermentation, adjusting the fermentation liquor and maintaining the pH value between 6.5 to 7.5; movably adding the aqueous solution of glycerin after the fermentation preformed for a period of time; the fermentation is finished when the content of glycerin stops increasing and is more than 35g / L; and then filtering the fermentation liquor through a pressure filter, getting the filtered solution, charging the filtered solution into a spray tower for spray drying, and the dried powder is the propionate product. The invention has the effects: the method can substitute the existing chemical synthesis to produce propionic acid, remarkably improving the safety of the propionic acid product, and is a green food additive; moreover, the problem of consumption of non-renewable resources (such as oil) can be solved without a secondary processing, the negative impact generated by chemical synthesis can be prevented, and the process flow and the cost can be saved; and the invention particularly opens up an industrialization stage of the propionic acid production by fermentation and the simultaneous production of the propionate.

The invention belongs to the field of cosmetics and skin care products and discloses an acne-removing composition, a skin care product and a preparation method thereof. The acne-removing composition comprises mangosteen peel, thyme and fructus aurantii. Bacteriostatic experiments of propionibacterium acnes prove that the traditional Chinese medicine composition is good in inhibitory effect on the propionibacterium acnes, the three medicinal materials of the composition have a synergic anti-inflammation effect, and the composition is fast to become effective and good in effect. The acne-removing skin care product comprises the acne-removing composition, oldenlandia diffusa, salviae miltiorrhizae, honeysuckle stem, Chinese honey locust seed, tea tree essential oil and the like. The acne-removing skin care product does not contain ethanol and is safe and mild. Experiments prove that the skin care product is evident in acne treating effect, high in cure rate, natural and mild and capable of being used for a long time. The preparation method of the skin care product is simple to operate and suitable for large-scale production.

The invention belongs to the technical field of cosmetics, and in particular relates to mild, oil-controlled and acne-removing essence. The multi-effect mild, oil-controlled and acne-removing essencefor men mainly comprises the following components: water, glycerinum, propylene glycol, EDTA disodium, xanthan gum, acryloyl dimethyl taurine / VP copolymer, allantoin, panthenol, a peony foot extract,p-hydroxyacetophenone, phenoxyethanol, ethylhexylglycerin, sodium benzoate, citric acid, o-cymene-5-alcohol, a phellodendron bark extract, butanediol, 10-hydroxydecanoate, a witchhazel extract, a lactic acid bacteria / mungbean seed extract fermented product filtrate, quaternary ammonium salt-73, PET-40 hydrogenated castor oil and essence. The mild, oil-controlled and acne-removing essence can effectively balance oil, reduce skin hyperkeratosis and minimize pores, can inhibit the growth of propionibacterium acnes and reduce skin inflammation such as acne, is mild and non-irritant and achieves the oil-controlling and acne-removing effects.

The invention discloses a polypeptide composition capable of effectively improving acne and repairing skin damage. The polypeptide composition comprises antibacterial polypeptides, polypeptides capable of inhibiting inflammatory response and / or polypeptides capable of promoting collagen and keratin regeneration and repairing acne marks. The polypeptide composition has the advantages that the growth and propagation of propionibacterium acnes and staphylococcus aureus can be inhibited, inflammatory response can be inhibited, edema and couperose skin can be prevented, corium layer collagen synthesis is increased, the regeneration and reinforcing of dermal-epidermis junction (DEJ) area structure tissue can be promoted, epidermis cell differentiation and maturation can be promoted, acne marks can be effectively repaired, and effective acne improving and skin damage repairing can be achieved.

The invention discloses a microbial agent for purifying sanitary sewage and a preparation method of the microbial agent. Active ingredients of the microbial agent is formed by mixing the following raw material bacteria including thiobacillus denitrificans, bacillus subtillis, bacillus licheniformis, bacillus polymyxa, lactic acid bacteria, nocardia amarae, denitrifying bacteria, rhodopseudomonas palustris, thiophilic red pseudomonas, rhodospirillum, saccharomycetes and propionibacterium. By means of the microbial agent, cellulose, saccharides, fat protein, pathogenic bacteria, viruses and parasitic ovum in sanitary sewage and pollutants, such as inorganic salt which is high in the contents of nitrogen, sulphur and phosphorus, the BOD and COD removal effect is guaranteed, meanwhile good bactericidal ability is achieved, high inhibiting ability is achieved especially for pathogenic bacteria, and the effect of treating sanitary sewage is remarkable.

The invention relates to application of a photosensitizer for treating acne. The photosensitizer is amino acid zinc phthalocyanine or a derivative thereof or organic or inorganic salt thereof which is used as the photosensitizer in photodynamic therapy for treating acne. The photosensitizer is of targeting and targeting aggressivity to target germs. Photons with special energy excite target molecules of the photosensitizer, the target molecules of the photosensitizer can transfer energy to oxygen molecules, and high energy oxygen molecules kill adjacent target germs: propionibacterium acnes, escherichia coli and staphylococcus aureus to treat acne. The photosensitizer provided by the invention used to treat acne is good in effect, free from antibiotics and hormone and tolerance, less in side effect, safe and nontoxic and economic and practical; can efficiently and quickly kill propionibacterium acnes, escherichia coli and staphylococcus aureus which induce acne to treat acne and restore skins without damaging normal cells; and is small in use level of effect medicines, and strong in effect of killing acne pathogenic bacteria.

The invention discloses a composite leavening agent combining xylanase with multiple strains and a method for fermenting a straw feed. The leavening agent consists of the following components in part by weight: 4 parts of xylanase, 2 parts of lactobacillus acidophilus, 2 parts of propionibacterium, 2 parts of saccharomyces cerevisiae, 3 parts of bacillus subtilis, 1 part of lactobacillus plantarum and 1 part of bacillus coagulans, wherein the xylanase can quickly degrade cell walls of straw plants and release nutrient substances; and under the synergistic effects of a plurality of microorganisms, fermentation speed is increased, fermentation period is shortened, fermentation activity is enhanced, protein conversion rate is increased and fermentation cost is lowered. The prepared fermented straw feed has low cellulose content, high protein content, high viable count, long retention period, soft and wet feel, strong acid incense, high palatability and high nutritional value. The problem of low resource utilization ratio of crops such as straws and the like is solved; simultaneously, environmental pollution is reduced; and the leavening agent and the method have high economic benefit and social benefit.

The present invention belongs to the technical field of cosmetics, and particularly relates to an acne removing and skin beautifying essence liquid and a preparation method thereof, wherein the essence liquid comprises an acne removing component, and the acne removing component comprises a phellodendron amurense tree bark extract and a rosa multiflora fruit extract. According to the present invention, the acne removing component is obtained through screening; the new acne removing component can effectively inhibit Propionibacterium acnes, and the test research results show that the product provides a good acne treating effect and a good whitening effect after the acne removing component is added to the essence liquid; and the stability research results show that the essence liquid has good stability.

The invention discloses an acne-removing composition and a preparation method thereof. The acne-removing composition can be absorbed by skins faster compared with an emulsion preparation so as to take the function to the maximum extent. The function of comprehensively removing acnes is achieved by means of improving the moisturizing effect of the skins, adjusting the grease balance of the skins, inhibiting hypersecretion of sebum and inhibiting reproduction of propionibacterium acnes as well as by virtue of interaction of anti-inflammatory action and acne removal. The pH of the acne-removing composition is 4.5-5.5 (10% aqueous solution) which is faintly acid, and the acne-removing composition is mild in property and does not irritate the skins, and meanwhile, the effects of macromolecular salicylic acid and a composite plant acne-removing preparation are more obvious.

The invention discloses an acne-removing cream containing Chinese herbal medicine extracts and a preparation method thereof, and aims to provide the acne-removing cream having stronger inhibitory effect on propionibacterium acnes, having good acne-removing effect, being safe and gentle and having no irritation on human bodies. The technical key point comprises that the acne-removing cream is prepared from the following components by the mass percentage: 6-10% of an emulsifier, 2-3% of a coemulsifier, 10-14% of grease, 0.4-0.6% of an antioxidant, 1.6-2.4% of an absorption enhancer, 5-7.5% of an acne-removing composition, 1.6-2.4% of an emulsifier thickener, 8-12% of a moisturizer, 0.2-1% of a preservative, and the balance water. With the total mass of the acne-removing composition as 100%, the acne-removing composition comprises the components by the mass percentage: 6-10% of camphor, 6-10% of menthol, 12-20% of a peony root extract, 28-40% of a rheum palmatum root extract, 20-30% of a Chinese gall extract and 8-12% of a cortex phellodendri amurensis extract. The acne-removing cream belongs to the technical field of skin care products.

The invention discloses an apple enzyme and a preparation method. The preparation method comprises the following steps: juicing apples, uniformly mixing apple juice with pure water, adding glucose which accounts for 1-5% of the total weight of the obtained raw material mixture into the raw material mixture that the volume percentage of the apple juice is 5-20%, adjusting the pH value to 5.0-7.0, sterilizing, preparing mixed bacteria from the obtained raw material liquid and a pichia pastoris bacterial liquid and a lactobacillus plantarum bacterial liquid in a volume ratio of 1:(1-5), controlling the temperature to 25-35 DEG C, performing primary fermentation for 12-48 hours, controlling the temperature of the obtained primary fermentation solution to 25-35 DEG C by using an acetic bacterial liquid, performing secondary fermentation for 12-36 hours, and sterilizing, thereby obtaining a finished product of the apple enzyme. Compared with a finished product of natural fermentation, the finished product of the apple enzyme disclosed by the invention has a remarkable antibacterial effect on propionibacterium acnes, and the removal rate IC50 of the apple enzyme on DPPH is 0.43%.

The present application relates to a dairy-derived probiotic composition comprising a propionic bacteria. The composition is particularly useful as a food supplement for normalizing the gastro-intestinal flora. The probiotic bacteria present in the composition contain species of Propionibacterium, Lactobacillus, Bifidobacterium and Streptococcus. Also disclosed herein are methods of producing such composition and uses of the composition.

The invention discloses a fibrous bed reactor and the equipment and process for extracting separation and coupling production of propionic acid. The invention utilizes an immobilized fibrous bed reactor unit, a membrane separation unit, an extraction and anti-extraction unit and an elution and condensation unit to realize the efficient production of propionic acid. The device has strong feasibility, simple operation, easy realization of automation, immobilized materials, long using life of the membrane and the matching equipment, convenient maintenance and good application prospect. Through extracting separation and coupling production technology, the separation of propionic acid is realized. The invention radically eliminates the feedback inhibition of propionic acid, implements the high density fermentation of propionibacterium and greatly improves the production efficiency of propionic acid through the fermentation method. The extracted water phase is returned to the reactor for recycle after elution and condensation by an elution device, which eliminates the harm of extractant for propionibacterium from the root.

The invention discloses a botanical medicine composition with an acne removing effect and application. The botanical medicine composition is prepared from radix salviae miltiorrhizae, pomegranate bark, spreading hedyotis herb, rhizoma curcumae and licorice roots. The botanical materials are smashed, screened and mixed, the mixture is added into an extraction solvent and then soaked, room-temperature extraction is carried out with a high-pressure homogenization extraction machine, extraction liquor is subjected to centrifugal separation and then concentrated with a membrane, concentrated liquor is adsorbed with macroporous resin and then eluted repeatedly with ethanol, elution liquor is collected, butanediol is added for dissolution after membrane concentration, filtering is carried out, and the botanical medicine composition is obtained, wherein water or ethanol or an ethanol aqueous solution serves as the extraction solvent. The botanical medicine composition can inhibit secretion of skin grease, effectively inhibit propionibacterium acnes and achieve a good acne removing effect, and can enhance skin immunity, improve skin nature and make skin permeable, clean, smooth and fine when used for a long term.

The invention relates to a strain marker for assisting COVID-19 diagnosis and application thereof and belongs to the technical field of pathogenic microorganism infection detection. The strain markercomprises at least one of haemophilus parainfluenzae, propionibacterium, veillonella atypica, aggregatibacter segnis, alloprevotellatannerae, campylobacter showae, fusobacteria, fusobacterium nucleatum, fusobacterium periodonticum, gemella sanguinis, haemophilus parahaemolyticus, haemophilus sputorum, porphyromonas gingivalis, prevotella intermedia, prevotella multifurmis, prevotella nanceiensis,prevotella nigrescens and prevotella timonensis. A prediction model ROC obtained by the strain marker has an AUC value being 0.910, and the strain marker can be used for assisting diagnosis of SARS-CoV-2 infection, so that the diagnosing performance on COVID-19 pneumonia can be improved, and the defect that viral nucleic acid detection is false negative can be overcome to a certain extent.

The invention provides a preparation method of a probiotic and aloe mask, which is characterized by preparing a fermentation stock solution from aloe vera raw juice, glucose and water according to certain proportions and adding probiotics for performing co-fermentation. By setting different temperature gradients and pH gradients, after viable count, optimal fermentation conditions are determined; an aloe and probiotic fermentation solution is adopted to carry out an antibacterial test so as to determine an antibacterial effect, such as propionibacterium acnes; and meanwhile, by determining an antioxidant index, the antioxidant ability of the aloe and probiotic fermentation solution is determined, such as determination of DPPH radical scavenging ability, determination of iron ion chelating ability and determination of total reducing force. According to the product, the aloe and probiotic fermentation solution is mixed with cosmetic auxiliary materials in proportion so as to be processed into a pasty mask, and after sensory index testing, skin irritation testing and trial effect testing, the safety and functionality of the product are determined, so that the aims of skin protection and health care are achieved.

The invention discloses a method for preparing antibacterial metabolin of Propionibacterium, the Propionibacterium (Propionibacterium.thoenii) is stored in American Type Culture Collection (ATCC) with the storing number of ATCC 4874. In the invention, the Propionibacterium is activated and fermented by microorganism fermentation technology to obtain the antibacterial metabolin containing the Propionibacterium. The antibacterial metabolin of Propionibacterium can be used in food anticorrosion and fresh-keeping, disinfector, anti-infection medicine, animal and plant disease control and the medicine field for inhibiting gram-negative bacteria, mould and microzyme. The method for preparing the antibacterial metabolin is convenient in operation, is low in production cost and has wide application prospect.

A method for treating an individual suffering from one of bladder cancer and colorectal cancer employs a CRISPR system to selectively kill or reduce the numbers of pathogenic bacteria within the individual and the individual is then administered an immune checkpoint inhibitor. In particular embodiments, the pathogenic bacteria is one of E. coli, Pseudomonas aeruginosa and Klebsiella bacteria, and the checkpoint inhibitor is selected from the group consisting of nivolumab, pembrolizumab, pidilizumab, AMP-224, AMP-514, STI-A1110, TSR-042, RG-7446, BMS-936559, MEDI-4736, MSB-0020718C, AUR-012 and STI-A1010. Further embodiments include enhancing the growth of a second bacteria in the individual, such bacteria including Akkermansia, Bacteroides, Bifidobacterium, Clostridium, Enterococcus, Fusobacterium, Lactobacillus, Propionibacterium, Ruminococcus, Veillonella, Prevotella, Escherichia and Streptococcus. Still other embodiments include increasing the levels of Roseburia and / or Faecalibacterium prausnitzii, in the individual's gut microbiome.

The invention provides Saccharomycopsis fibuligera with the collection number of CCTCC M 2019324. Tests prove that the Saccharomycopsis fibuligera CCTCC M2019324 has DPPP free radical scavenging rateof an extracellular supernatant is 81.9% and a tyrosinase inhibition rate of 68.1%. The extracellular supernatant of Saccharomycopsis fibuligera CCTCC M2019324 is fermented with a medicinal plant as asubstrate, and a fermentation liquid of the extracellular supernatant has a better acne propionibacterium inhibition effect by detection. Meanwhile, the medicinal plant fermentation liquid can also be applied to preparation of cosmetics, and a prepared product of the plant fermentation liquid containing the e Saccharomycopsis fibuligera CCTCC M2019324 can effectively scavenge free radicals, promote skin renewal and reduce synthesis of melanin and has very good application prospects.

A method for treating an individual suffering from a chronic infectious disease and who has cancer employs a CRISPR system to selectively kill or reduce the numbers of pathogenic bacteria within the individual and the individual is then administered an immune checkpoint inhibitor. In particular embodiments, the pathogenic bacteria is one of E. coli, Pseudomonas aeruginosa and Klebsiella bacteria, and the checkpoint inhibitor is selected from the group consisting of nivolumab, pembrolizumab, pidilizumab, AMP-224, AMP-514, STI-A1110, TSR-042, RG-7446, BMS-936559, MEDI-4736, MSB-0020718C, AUR-012 and STI-A1010. Further embodiments include enhancing the growth of a second bacteria in the individual, such bacteria including Akkermansia, Bacteroides, Bifidobacterium, Clostridium, Enterococcus, Fusobacterium, Coprococcus, Lactobacillus, Propionibacterium, Ruminococcus, Veillonella, Prevotella, Escherichia and Streptococcus. The CRISPR system may include Cas9, Cpf1 and Cas3, and may be delivered using a bacteriophage.

The invention discloses acne-removing cream. The acne-removing cream is prepared from hexamidine diisethionate, a hydroxypropyl-beta-cyclodextrin inclusion compound of azelaic acid, a hydroxypropyl-beta-cyclodextrin inclusion compound of salicylic acid and oat alkaloid derivatives. Permeation and stripping effects of salicylic acid in the hydroxypropyl-beta-cyclodextrin inclusion compound of salicylic acid is beneficial to permeation of other components into the skin basal layer, and the hexamidine diisethionate, a hydroxypropyl-beta-cyclodextrin inclusion compound of azelaic acid are enabledto more effectively kill propionibacterium acnes to fundamentally treat acnes, and great acne removing effects are achieved. By the hydroxypropyl-beta-cyclodextrin inclusion compound of azelaic acid,oil secretion and chromatosis can be evidently reduced while antibacterial acne-removing effects are achieved, water-oil balance is effectively regulated, and acne reduction and acne mark fading are realized; by the oat alkaloid derivatives, skin irritation symptoms such as allergy and stabbing pain caused by other components can be alleviated, and swelling, itching and pains of acne affected parts can be alleviated.

The present invention relates to the use of microorganisms Lactobacillus rhamnosus LGG, ATCC 53103, Lactobacillus rhamnosus LC705, DSM 7061 and Propionibacterium freundii subsp. Sherbyi PJS, DSM 7067 for the inhibition of yeast growth, prevention and treatment of yeast in animals or humans Caused diseases and relief of yeast-related symptoms.

The invention discloses a sterilization composition for regulating skin microorganism floras and an application of the sterilization composition. The sterilization composition consists of a lactobacilli fermentation lysis product, quaternary ammonium salt-73, and a mangosteen peel extract. For the composition, the lactobacilli fermentation lysis product and the mangosteen peel extract can reduce killing rate of beneficial bacteria or symbiotic bacteria when other small pox removing components (such as the quaternary ammonium salt-73) are used for killing Propionibacterium acnes, and unbalanceof different degrees of floras caused by individual difference is balanced. In addition, the collocation use of the lactobacilli fermentation lysis product and the mangosteen peel extract can also effectively reduce stimulation or damage of conventional preservatives to faces of acne patients, and can reduce generation of allergic reaction situations that faces are itching, painful and swollen caused by preservatives.

The invention provides a polypeptide having the molecular weight of 3321.6 Da and having an amino acid sequence as shown in SEQ ID NO.1. The invention also provides six derivatives of the polypeptide. The polypeptide and the derivatives thereof provided by the invention have efficient broad-spectrum bacteriostatic effect on gram positive bacteria and gram negative bacteria (especially having good killing effect on anti-clindamycin anaerobic propionibacterium acnes), fungi and yeasts, and have high activity (the concentration for killing the propionibacterium acnes in vitro can be only 0.125 mg / L). The polypeptide and the derivatives thereof can be applied in preparation of microbial bacterial and fungal infection-controlling and anti-inflammatory products, wherein the products include externally-applied disinfectants, anti-bacterial agents, wound-protection or infection-prevention dressings, acne treating drugs, skin care products, cosmetics and the like. The polypeptide is designed and synthesized based on a tryptophan bromide-containing fragment of a marine biological sea urchin, has stronger bacteria inhibiting or killing and anti-inflammatory effects and better stability, and can be used as an antibiotic substitute.