469 results about "Pure culture" patented technology

A biologically pure culture of a yeast of the species Metschnikowia fructicola. The yeast is identified as NRRL Y-30752 and is capable of inhibiting growth of a deleterious micro-organism on a portion of a plant to which a biologically effective amount of a culture of the yeast is applied. Further disclosed is a composition for use in protection of agricultural produce including a biologically effective amount of Metschnikowia fructicola and a carrier. Further disclosed is an article of manufacture including packaging material and the disclosed composition which is identified for use in protection of agricultural produce from a deleterious micro-organism. Further disclosed is a method of inhibiting growth of a deleterious micro-organism on a portion of a plant including applying at least one time an agriculturally effective amount of yeast of the genus Metschnikowia to the portion of a plant.

A process for the heterotrophic or predominantly heterotrophic production of whole-celled or extracted microbial products with a high concentration of omega-3 highly unsaturated fatty acids, producible in an aerobic culture under controlled conditions using biologically pure cultures of heterotrophic single-celled fungi microorganisms of the order Thraustochytriales. The harvested whole-cell microbial product can be added to processed foods as a nutritional supplement, or to fish and animal feeds to enhance the omega-3 highly unsaturated fatty acid content of products produced from these animals. The lipids containing these fatty acids can also be extracted and used in nutritional, pharmaceutical and industrial applications.

The invention relates to one or more systems and methods for creating a mixture of cultures used to replace intestinal flora. Specifically, the invention relates to methods and systems of treating diseases including Clostridium difficile and Crohn's disease by introducing a mixture of pure cultures of viable bacteria into the gastrointestinal tract.

The invention enables efficient, rapid, and sensitive enumeration of living cells by detecting microscopic colonies derived from in situ cell division using large area imaging. Microbial enumeration tests based on the invention address an important problem in clinical and industrial microbiology—the long time needed for detection in traditional tests—while retaining key advantages of the traditional methods based on microbial culture. Embodiments of the invention include non-destructive aseptic methods for detecting cellular microcolonies without labeling reagents. These methods allow for the generation of pure cultures which can be used for microbial identification and determination of antimicrobial resistance.

A process for the heterotrophic or predominantly heterotrophic production of whole-celled or extracted microbial products with a high concentration of omega-3 highly unsaturated fatty acids, producible in an aerobic culture under controlled conditions using biologically pure cultures of heterotrophic single-celled fungi microorganisms of the order Thraustochytriales. The harvested whole-cell microbial product can be added to processed foods as a nutritional supplement, or to fish and animal feeds to enhance the omega-3 highly unsaturated fatty acid content of products produced from these animals. The lipids containing these fatty acids can also be extracted and used in nutritional, pharmaceutical and industrial applications.

The invention discloses preparation process for fermented chilli sauce. The preparation process includes the following steps: placing fresh chilli and ginger into a jar, mixing cultures of lactobacillus planetarium and fermentation lactobacillus, and conducting three times of culture cultivation to obtain fermentation broth; adding the fermentation broth and fermenting; taking the chilli and ginger out of the jar and pulping; stirring and seasoning; packaging the fermented chilli sauce with tins or toothpaste-tube-shaped jars for replacing the jars and vacuum-sealing; and sterilizing through heat to obtain finished products. The chilli sauce is produced through pure-culture fermentation, calcium lactate and calcium chloride are used as a hardener, and color protecting processing is conducted by using 0.1% erythorbic acid combined with 0.1% laurel kojic acid. Simultaneously, the chilli sauce is packaged by using the tins and toothpaste-tube-shaped cans and has the advantages of being short in fermenting time, low in nitrite content, stable in product quality and good in product appearance quality.

The invention describes application of pure culture microbial strains under denitrifying conditions for growth on crude oil thereby modifying crude oil properties to enhance its recovery.

The present invention relates to a probiotic bacterial strain belonging to the genus Lactobacillus having the ability to colonize the human vagina, or a variant thereof. More specifically the probiotic bacterial strain belongs to a species chosen from the group comprising Lactobacillus plantarum, Lactobacillus crispatus, and Lactobacillus gasseri. Further it relates to its use as a medicament, a composition comprising the strain, the composition, e.g., being a food product or a pharmaceutical composition, a hygiene product, a biological pure culture of the strain, and a novel food.

The invention enables efficient, rapid, and sensitive enumeration of living cells by detecting microscopic colonies derived from in situ cell division using large area imaging. Microbial enumeration tests based on the invention address an important problem in clinical and industrial microbiology—the long time needed for detection in traditional tests—while retaining key advantages of the traditional methods based on microbial culture. Embodiments of the invention include non-destructive aseptic methods for detecting cellular microcolonies without labeling reagents. These methods allow for the generation of pure cultures which can be used for microbial identification and determination of antimicrobial resistance.

A process for the heterotrophic or predominantly heterotrophic production of whole-celled or extracted microbial products with a high concentration of omega-3 highly unsaturated fatty acids, producible in an aerobic culture under controlled conditions using biologically pure cultures of heterotrophic single-celled fungi microorganisms of the order Thraustochytriales. The harvested whole-cell microbial product can be added to processed foods as a nutritional supplement, or to fish and animal feeds to enhance the omega-3 highly unsaturated fatty acid content of products produced from these animals. The lipids containing these fatty acids can also be extracted and used in nutritional, pharmaceutical and industrial applications.

The invention discloses an efficient microbial soil remediation agent, belonging to a propagation culture technique of microbes. The efficient microbial soil remediation agent is prepared by mixing the following components in percentage by volume: 10-25% of photosynthetic bacteria zymogen solution, 25-35% of Bacillus subtilis zymogen solution, 20-35% of microzyme zymogen solution and 20-40% of actinomycetes zymogen solution. The four strains are respectively subjected two secondary amplification culture under the conditions of proper temperature, ventilation, culture medium, pH value and culture time; and the culture process is carried out in a fermentation tank without foreign microbes. By using the modern biotechnology, single microbe strains are subjected to high-concentration pure culture and then compounded to produce the efficient microbial soil remediation agent.

The invention provides corn straw nano dietary fiber and a preparation method thereof. The method adopts cellulase-producing bacteria for pure culture or mixed bacteria cofermentation to ferment corn straws, and carries out the ultra-high pressure homogenization treatment on the fermented corn straws to obtain the nano dietary fiber through spray drying or freeze drying. The corn straws are washed by water to remove impurities, dried, crushed and sieved, mixed evenly with skim milk powder or other substances and the water, sterilized and cooled; and a single-bacterium or multi-bacteria seed liquid of the cellulase-producing bacteria is inoculated into a culture medium for fermentation, a product after the fermentation is rinsed until the pH value is neutral, and the corn straw nano dietary fiber is prepared after homogenization treatment and spray drying or freeze drying. The method has simple preparation technology, mild conditions and no pollution to the environment, and is easy for industrialized production; and the dietary fiber has exquisite taste, increases the solubility, greatly improves water retention ability, water absorptivity and expansive power, and improves the content of soluble dietary fiber by more than 50 percent.

The present invention relates a method for isolating four cell types from a single umbilical cord as pure cultures. These cell lines (epithelial cells, fibroblasts, smooth muscle cells and endothelial cells) can be characterised and utilised in experimental models and for therapeutic purposes. Particularly, the umbilical cells isolated herein are used to form a tissue replacement or engineered living composition. Also, the isolated umbilical cells of the invention may have the potential of progenitor cells.

Provided are novel processes for the efficient production of L-epi-2-inosose and epi-inositol which are useful either as various medicines or intermediates for the syntheses of various medicines. In the processes, inexpensive myo-inositol is used as a starting compound which is reacted with a gram-negative bacterium capable of converting myo-inositol into L-epi-2-inosose, and thereby producing L-epi-2-inosose by conversion of myo-inositol into L-epi-2-inosose. A biologically pure culture of Pseudomonas sp. AB 10215 strain is also provided which has a characteristic nature of being capable of converting myo-inositol into L-epi-2-inosose.

The invention discloses a method for processing natto flavoring paste which is suitable for Chinese. The method comprises the following steps of: soaking, cleaning and cooking soybean; performing pure culture of strains; inoculating; performing primary fermentation; performing secondary refrigerating fermentation; and seasoning to obtain the finished product. Due to a temperature shift fermentation mode, the activity of nattokinase is ensured after natto is fermented, lactobacillus acidophilus generates acid to neutralize partial fermentation ammoniacal odor, and the product does not have peculiar smell which is not liked by Chinese, and has special aroma, pleasant flavor and smooth mouthfeel. The method has the advantages of reasonable process, simple operation, short process period and good flavor of the natto, the natto flavoring paste meets the requirements of Chinese on mouthfeel and flavor, industrial production can be realized, the process is controllable, the quality is stable, the benefits of soybean product processing enterprisers can be improved, and lactic acid bacteria are added to further enhance the health-care effect of the natto product; and the invention provides a feasible method for promoting the natto product in China.

The invention discloses a treatment process of antibiotic pharmacy waste water, which comprises the steps of pretreatment of the antibiotic pharmacy waste water, immobilized bio-enzyme treatment, immobilized activated sludge treatment and the like. The method enables the immobilized enzyme technology and the immobilized activated sludge technology to be combined and applied to treatment of the antibiotic waste water and improves the effects by 2-3 times compared with a method singly using an immobilized microorganism technology or a biological reinforcement technology. The method does not usespecial-effect genetic engineering bacteria which antibiotics can tolerate or natural bacteria subjected to pure culture and separation, and the environmental risks of formation and diffusion of superbacteria are reduced.

Polynucleotide primers and probes for the amplification and detection of E. coli O157 in a test sample are provided. The primers and probes can be used in real time diagnostic assays for rapid detection of E. coli O157 in a variety of situations, including clinical samples, microbiological pure cultures, food, and environmental and pharmaceutical quality control processes. Kits comprising the primers and probes are also provided.

The invention provides a lactobacillus paraplantarum capable of reducing cholesterol and nitrite. The lactobacillus paraplantarum is separated from traditional fermented preserved vegetables of minority nationalities in Guizhou, and is named as Lactobacillus paraplantarum GUFHSL-70, and lactobacillus paraplantarum pure culture is collected by CCTCC (China Center For Type Culture Collection) with the collection number of CCTCC M 2016002 in Wuhan University, Wuhan, China on Jan. 1 2016. The lactobacillus paraplantarum GUFHSL-70 is functional lactobacillus which has favorable characteristic in the aspect of reducing cholesterol and nitrite; through developing the lactobacillus paraplantarum GUFHSL-70 into health foods or medicine and taking the health foods or medicine for a long term, intestinal flora can be improved, serum cholesterol is reduced, and the immunity of the organism is improved; or by virtue of adding the lactobacillus paraplantarum GUFHSL-70 into the fermented preserved vegetables, the amount of nitrate residues in the preserved vegetables is reduced, the fermentation period is shortened, and the product flavor is improved.

The invention relates to a multiple PCR (polymerase chain reaction) method for identifying salmonella enteritidis, salmonella typhimurium, salmonella pullorum and salmonella gallinarum. The multi-PCR method comprises the following steps of: with the extracted DNA (deoxyribonucleic acid) of a bacterium to be detected as a template, carrying out mPCR (multiple polymerase chain reaction) amplification on five pairs of primers as shown in SEQ ID NO.1-10; and carrying out agarose gel electrophoresis on mPCR amplification products, and then determining according to electrophoresis results. According to the multiple PCR method, the synthesized primers are used for carrying out PCR amplification, a diseased material can be directly ground and amplified by adopting the established multiple PCR, different salmonella serotypes can be detected in one system, serum glass plate agglutination is completed in only about six hours compared with three days for the traditional serum glass plate agglutination after separation pure culture in clinical detection, the diagnosis time is greatly shortened, and a basis is provided for clinical diagnosis.

Provided is fragrant pig derived cholesterol-assimilating and oxygen-resistant bifidobacterium BZll. The fragrant pig derived cholesterol-assimilating and oxygen-resistant bifidobacterium BZll is Bifidobacterium animalis subsp.Lactis, the pure culture of the fragrant pig derived cholesterol-assimilating and oxygen-resistant bifidobacterium BZll is preserved at the China General Micro-biological Culture Collection Center (CGMCC), China Microbial Culture Preservation Management Committee, the address is Institute of Microbiology, Institution No.3 China Academy of Science, Western Beichen Road No.1 in Chaoyang District of Beijing in China, the postal code is 100101, the preservation number is CGMCC NO.10224, and the Bifidobacterium animalis subsp.Lactis BZll is called for short. The Bifidobacterium animalis subsp.Lactis BZll is high in cholesterol-assimilating capacity, acid resistance and cholate resistance, good in oxygen resisting performance, has probiotic property and can serve as probiotics to be added into a fermentative milk product to produce a functional food, bifidobacterium culture resources are enriched, and development of bifidobacterium health-care products is facilitated.

The invention discloses a production method for high-density pure arbuscular mycorrhizal fungal spore, which belongs to the technical field of microorganism culturing, and comprises the following technical steps of: 1) culturing carrot root tissues converted from agrobacterium rhizogenes plasmid DNA; 2) culturing the carrot root tissues converted from agrobacterium rhizogenes plasmid DNA and glomus intraradices together in an improved synthetic culture medium; 3) taking a culture containing the root, hypha and spore infected by the arbuscular mycorrhizal fungus and transplanting the culture to a special culture box for culturing; 4) collecting the spore and mycelium; and 5) storing the arbuscular mycorrhizal fungal spore. Compared with the prior art, the production method for high-density pure arbuscular mycorrhizal fungal spore has the advantages that: 1, the culture medium is an asepsis environment with low cost, which not only can meet the growing of a host plant, but also is suitable for the growing of AM epiphyte; 2, the nutrition requirements for the growing of the host plant are low and the host plant can grow and be cultured on the synthetic culture medium; 3, the spore cultured by the method is a non-pollution pure culture; and 4, the produced spore is stored in liquids and the activity can be maintained by above 90 percent.

The present invention relates generally to the field of health promoting agents, in particular antimicrobial agents and provides antimicrobial peptides and bacterial strains that provide the antimicrobial peptides. In one aspect, the invention provides a biologically pure culture of Lactobacillus acidophilus, strain DPC6026, a sample of which has been deposited at the National Collection of Industrial and Marine Bacteria, Aberdeen, Scotland on 18th Nov. 2005 under the accession number NCIMB 41354, or a derivative or mutant thereof capable of producing from milk or a milk product, peptides having antimicrobial activity.

The invention relates to a pure-culture solid-state fermentation apparatus capable of gas-phase inoculation and feeding, and a method thereof, and the apparatus is characterized in that the apparatus comprises a venturi tube, wherein the inlet end of the venturi tube is connected with an air filter through an air circuit valve; the outlet end of the venturi tube is connected with a solid-state fermentation cylinder which is provided with a pressure release valve; the throat segment of the venturi tube is connected with the outlet ends of a seed pot and a feeding pot through the valves of outlet ends of the seed pot and the feeding pot, respectively; the air circuit between the air circuit valve and the venturi tube is connected with a steam generator, the inlet end of the feeding pot, and the inlet end of the liquid seed pot through valves of the air circuit ends of the stream generator, the feeding pot, and the liquid seed pot, respectively; when high speed air flows through the venturi tube, the air-flow speed at the throat segment of the venturi tube increases to form a negative pressure to absorb the liquid seed or material supplement; and the liquid seed or feed liquid is mixed with the gas to form aerosol, which is injected into the solid substrate in the solid fermentation cylinder, and aseptic inoculation and aseptic feeding are finished.

A substantially pure culture of a microorganism having enzyme activities of pyrG and CCT and carrying a recombinant DNA composed of a DNA fragment containing genes encoding pryG and CCT and a vector.

The present invention describes methods for the detoxification of a mixture of nitrile compounds, or a mixture of nitrile and amide compounds by conversion of the nitrile compound(s) to the corresponding amide or acid compounds using a pure culture of an induced microorganism strain capable of converting a nitrile moiety to an amide or acid moiety. If an amide is formed or is present in the mixture, the amide can be further converted, using the present methods for detoxification, to the corresponding acid. The acid can then, if desired, be further degraded to CO2, H2O and biomass. The induced pure cultures are able to detoxify a mixture of nitriles or a mixture of nitriles and amides which are typically present, in high concentration(s), in nitrile production waste streams. The present invention further discloses methods for removing a nitrile compound from an amide preparation, such as an acetamide or acrylamide preparation containing an unwanted nitrile compound, using an induced pure culture of an induced microorganism strain capable of converting a nitrile moiety to an amide or acid moiety. The pure cultures are able to purify or reduce the toxicity of the amide preparation thus improving purity and amide product yield from the amide preparation. The present invention further discloses methods for the conversion of a mixture of amide compounds to the corresponding acid compounds using a pure culture of an induced microorganism strain capable of converting an amide moiety to an acid moiety. This invention also discloses kits, biofilters and methods for use of the kits and biofilters for detoxification containing the useful microorganism strains.

The present invention provides methods for generating oligodendrocyte progenitor cells from pluripotent cells, as well as methods for sustaining these oligodendrocyte progenitor cells in relatively pure cultures for long periods of time. The present invention also provides methods for further differentiating these oligodendrocyte progenitor cells into various glial cells.

The invention belongs to the technical field of tea leaf processing, and discloses a preparation method of golden flower white tea cake tea. The preparation method comprises the steps of culturing by utilizing a golden flower fungus suspension prepared through leaching tea leaves rich in golden flower fungus, and the golden flower fungus adopting white tea loose tea as a matrix; then mixing the tea leaves obtained through pure culture and the white tea loose tea according to a certain proportion, and steaming and pressing by adopting a certain form to form a white tea cake or tea brick; promoting the white tea cake (brick) to generate golden flowers under a certain environment, wherein the number of eurotium cristatum in the obtained golden flower white tea cake is larger than or equal to 20*10<4>CFU / g, and the number of eurotium cristatum in the golden flower white tea brick is larger than or equal to 50*10<4>CFU / g. According to the preparation method of the golden flower white tea cake tea provided by the invention, Yunnan white tea is adopted as a raw material for the first time, the white tea cake (brick) is promoted to naturally generate the golden flowers through processes such as exogenous inoculation, and steaming and pressing to form the cake, so that an original quality characteristic of the white tea is improved, the white tea not only has an original health-care effect, but also has the health-care effects on reducing blood sugar and blood fat, and has a wide application prospect.

The invention discloses a construction method of a microbial peptide mass fingerprint spectrum database based on a time-of-flight mass spectrometry principle, wherein the method comprises the steps: carrying out pure culture of obtained strains according to physiological habits, and successively obtaining strains at logarithmic phase; identifying the strains through morphology, taxonomy and methodology; then pretreating the confirmed strains, drying, and then extracting peptide fingerprint spectra by an ethanol / formic acid method and an 80% TFA extraction method; collecting and treating the spectra by adopting an MALDI-TOF MS platform, then carrying out parameter determination of the strain spectra, unifying the determined standard spectra and strain names, labeling a database construction method of the strains, and completing the database construction of the strains; and analogizing by the construction methods of the other strains successively, and completing the construction of the microbial peptide mass fingerprint spectrum database. The invention provides a set of complete method from the investigation of the strains to the construction of the database. The database has complete types of microorganisms, and meets identification requirements of microorganism related industries.