467 results about "Pure culture" patented technology

The invention relates to one or more systems and methods for creating a mixture of cultures used to replace intestinal flora. Specifically, the invention relates to methods and systems of treating diseases including Clostridium difficile and Crohn's disease by introducing a mixture of pure cultures of viable bacteria into the gastrointestinal tract.

The invention enables efficient, rapid, and sensitive enumeration of living cells by detecting microscopic colonies derived from in situ cell division using large area imaging. Microbial enumeration tests based on the invention address an important problem in clinical and industrial microbiology—the long time needed for detection in traditional tests—while retaining key advantages of the traditional methods based on microbial culture. Embodiments of the invention include non-destructive aseptic methods for detecting cellular microcolonies without labeling reagents. These methods allow for the generation of pure cultures which can be used for microbial identification and determination of antimicrobial resistance.

A process for the heterotrophic or predominantly heterotrophic production of whole-celled or extracted microbial products with a high concentration of omega-3 highly unsaturated fatty acids, producible in an aerobic culture under controlled conditions using biologically pure cultures of heterotrophic single-celled fungi microorganisms of the order Thraustochytriales. The harvested whole-cell microbial product can be added to processed foods as a nutritional supplement, or to fish and animal feeds to enhance the omega-3 highly unsaturated fatty acid content of products produced from these animals. The lipids containing these fatty acids can also be extracted and used in nutritional, pharmaceutical and industrial applications.

The invention describes application of pure culture microbial strains under denitrifying conditions for growth on crude oil thereby modifying crude oil properties to enhance its recovery.

A process for the heterotrophic or predominantly heterotrophic production of whole-celled or extracted microbial products with a high concentration of omega-3 highly unsaturated fatty acids, producible in an aerobic culture under controlled conditions using biologically pure cultures of heterotrophic single-celled fungi microorganisms of the order Thraustochytriales. The harvested whole-cell microbial product can be added to processed foods as a nutritional supplement, or to fish and animal feeds to enhance the omega-3 highly unsaturated fatty acid content of products produced from these animals. The lipids containing these fatty acids can also be extracted and used in nutritional, pharmaceutical and industrial applications.

The present invention relates a method for isolating four cell types from a single umbilical cord as pure cultures. These cell lines (epithelial cells, fibroblasts, smooth muscle cells and endothelial cells) can be characterised and utilised in experimental models and for therapeutic purposes. Particularly, the umbilical cells isolated herein are used to form a tissue replacement or engineered living composition. Also, the isolated umbilical cells of the invention may have the potential of progenitor cells.

The invention discloses a method for processing natto flavoring paste which is suitable for Chinese. The method comprises the following steps of: soaking, cleaning and cooking soybean; performing pure culture of strains; inoculating; performing primary fermentation; performing secondary refrigerating fermentation; and seasoning to obtain the finished product. Due to a temperature shift fermentation mode, the activity of nattokinase is ensured after natto is fermented, lactobacillus acidophilus generates acid to neutralize partial fermentation ammoniacal odor, and the product does not have peculiar smell which is not liked by Chinese, and has special aroma, pleasant flavor and smooth mouthfeel. The method has the advantages of reasonable process, simple operation, short process period and good flavor of the natto, the natto flavoring paste meets the requirements of Chinese on mouthfeel and flavor, industrial production can be realized, the process is controllable, the quality is stable, the benefits of soybean product processing enterprisers can be improved, and lactic acid bacteria are added to further enhance the health-care effect of the natto product; and the invention provides a feasible method for promoting the natto product in China.

The invention discloses a treatment process of antibiotic pharmacy waste water, which comprises the steps of pretreatment of the antibiotic pharmacy waste water, immobilized bio-enzyme treatment, immobilized activated sludge treatment and the like. The method enables the immobilized enzyme technology and the immobilized activated sludge technology to be combined and applied to treatment of the antibiotic waste water and improves the effects by 2-3 times compared with a method singly using an immobilized microorganism technology or a biological reinforcement technology. The method does not usespecial-effect genetic engineering bacteria which antibiotics can tolerate or natural bacteria subjected to pure culture and separation, and the environmental risks of formation and diffusion of superbacteria are reduced.

Polynucleotide primers and probes for the amplification and detection of E. coli O157 in a test sample are provided. The primers and probes can be used in real time diagnostic assays for rapid detection of E. coli O157 in a variety of situations, including clinical samples, microbiological pure cultures, food, and environmental and pharmaceutical quality control processes. Kits comprising the primers and probes are also provided.

The invention provides a lactobacillus paraplantarum capable of reducing cholesterol and nitrite. The lactobacillus paraplantarum is separated from traditional fermented preserved vegetables of minority nationalities in Guizhou, and is named as Lactobacillus paraplantarum GUFHSL-70, and lactobacillus paraplantarum pure culture is collected by CCTCC (China Center For Type Culture Collection) with the collection number of CCTCC M 2016002 in Wuhan University, Wuhan, China on Jan. 1 2016. The lactobacillus paraplantarum GUFHSL-70 is functional lactobacillus which has favorable characteristic in the aspect of reducing cholesterol and nitrite; through developing the lactobacillus paraplantarum GUFHSL-70 into health foods or medicine and taking the health foods or medicine for a long term, intestinal flora can be improved, serum cholesterol is reduced, and the immunity of the organism is improved; or by virtue of adding the lactobacillus paraplantarum GUFHSL-70 into the fermented preserved vegetables, the amount of nitrate residues in the preserved vegetables is reduced, the fermentation period is shortened, and the product flavor is improved.

Provided is fragrant pig derived cholesterol-assimilating and oxygen-resistant bifidobacterium BZll. The fragrant pig derived cholesterol-assimilating and oxygen-resistant bifidobacterium BZll is Bifidobacterium animalis subsp.Lactis, the pure culture of the fragrant pig derived cholesterol-assimilating and oxygen-resistant bifidobacterium BZll is preserved at the China General Micro-biological Culture Collection Center (CGMCC), China Microbial Culture Preservation Management Committee, the address is Institute of Microbiology, Institution No.3 China Academy of Science, Western Beichen Road No.1 in Chaoyang District of Beijing in China, the postal code is 100101, the preservation number is CGMCC NO.10224, and the Bifidobacterium animalis subsp.Lactis BZll is called for short. The Bifidobacterium animalis subsp.Lactis BZll is high in cholesterol-assimilating capacity, acid resistance and cholate resistance, good in oxygen resisting performance, has probiotic property and can serve as probiotics to be added into a fermentative milk product to produce a functional food, bifidobacterium culture resources are enriched, and development of bifidobacterium health-care products is facilitated.

The present invention relates generally to the field of health promoting agents, in particular antimicrobial agents and provides antimicrobial peptides and bacterial strains that provide the antimicrobial peptides. In one aspect, the invention provides a biologically pure culture of Lactobacillus acidophilus, strain DPC6026, a sample of which has been deposited at the National Collection of Industrial and Marine Bacteria, Aberdeen, Scotland on 18th Nov. 2005 under the accession number NCIMB 41354, or a derivative or mutant thereof capable of producing from milk or a milk product, peptides having antimicrobial activity.

The invention relates to a pure-culture solid-state fermentation apparatus capable of gas-phase inoculation and feeding, and a method thereof, and the apparatus is characterized in that the apparatus comprises a venturi tube, wherein the inlet end of the venturi tube is connected with an air filter through an air circuit valve; the outlet end of the venturi tube is connected with a solid-state fermentation cylinder which is provided with a pressure release valve; the throat segment of the venturi tube is connected with the outlet ends of a seed pot and a feeding pot through the valves of outlet ends of the seed pot and the feeding pot, respectively; the air circuit between the air circuit valve and the venturi tube is connected with a steam generator, the inlet end of the feeding pot, and the inlet end of the liquid seed pot through valves of the air circuit ends of the stream generator, the feeding pot, and the liquid seed pot, respectively; when high speed air flows through the venturi tube, the air-flow speed at the throat segment of the venturi tube increases to form a negative pressure to absorb the liquid seed or material supplement; and the liquid seed or feed liquid is mixed with the gas to form aerosol, which is injected into the solid substrate in the solid fermentation cylinder, and aseptic inoculation and aseptic feeding are finished.

The invention discloses a construction method of a microbial peptide mass fingerprint spectrum database based on a time-of-flight mass spectrometry principle, wherein the method comprises the steps: carrying out pure culture of obtained strains according to physiological habits, and successively obtaining strains at logarithmic phase; identifying the strains through morphology, taxonomy and methodology; then pretreating the confirmed strains, drying, and then extracting peptide fingerprint spectra by an ethanol/formic acid method and an 80% TFA extraction method; collecting and treating the spectra by adopting an MALDI-TOF MS platform, then carrying out parameter determination of the strain spectra, unifying the determined standard spectra and strain names, labeling a database construction method of the strains, and completing the database construction of the strains; and analogizing by the construction methods of the other strains successively, and completing the construction of the microbial peptide mass fingerprint spectrum database. The invention provides a set of complete method from the investigation of the strains to the construction of the database. The database has complete types of microorganisms, and meets identification requirements of microorganism related industries.