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Method of isolating cells from umbilical cord

a technology of umbilical cord and isolating cells, which is applied in the field of isolating cells from the umbilical cord, can solve the problem that there are no equivalent techniques in the art, and achieve the effect of reducing the number of stents

Inactive Publication Date: 2006-09-07
UNIV LAVAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

No equivalent techniques in the art are known to allow a skilled person to isolate four cell types from a single tissue biopsy without significant cell mortality and tissue destruction.

Method used

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  • Method of isolating cells from umbilical cord
  • Method of isolating cells from umbilical cord
  • Method of isolating cells from umbilical cord

Examples

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example ii

Characterisation of Cell Cultures

Cells in Culture

[0055] All four cell types could be established and maintained in culture using the aforementioned combination of extraction techniques. Each cell type has its characteristic phenotype and proliferation profile, as well as its particular requirements insofar as culture conditions. In FIG. 1, the typical aspects of the different cell types in monolayer culture are illustrated; in a) endothelial cells, in b) smooth muscle cells, in c) fibroblasts, and in d) a proliferative colony of epithelial cells surrounded by feeder S3T3 cells. All photographs were taken at 10× magnification under phase contrast.

[0056] Problems encountered with the extractions were not directly related to the extraction technique itself, but resulted from a variation of the properties of tissues and cells between different individuals. These problems included blockages in the vein which did not permit perfusion, weak spots in the vein wall which burst under pre...

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Abstract

The present invention relates a method for isolating four cell types from a single umbilical cord as pure cultures. These cell lines (epithelial cells, fibroblasts, smooth muscle cells and endothelial cells) can be characterised and utilised in experimental models and for therapeutic purposes. Particularly, the umbilical cells isolated herein are used to form a tissue replacement or engineered living composition. Also, the isolated umbilical cells of the invention may have the potential of progenitor cells.

Description

TECHNICAL FIELD [0001] The present invention relates to a method for isolating four cell types from the umbilical cord, and more particularly from the umbilical vein, in good conditions without having to execute separate methods for each type of cell. The method is of particular importance for obtaining a variety of different cells in good condition from only one tissue. BACKGROUND ART [0002] Despite the diversity of the nature, morphology, characteristics and function of isolated animal cells, it is presently generally admitted that these cells are derived from a single cell population, termed “stem cells”. Unlike more mature or differentiated cells, stem cells are capable of self-regeneration but may also divide into progenitor cells that are no longer pluripotent nor capable of self-regeneration. These progenitor cells divide repeatedly to form more mature cells, which eventually become terminally differentiated to form various mature cells. Thus the large number of mature cells ...

Claims

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Application Information

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IPC IPC(8): C12N5/08C12N5/071C12N5/073C12N5/077
CPCC12N5/0605C12N5/0629C12N5/0652C12N5/069C12N5/0691C12N2509/00
Inventor AUGER, FRANCOIS A.GERMAIN, LUCIEREMY-ZOLGHADRI, MURIELLEHAYWARD, CINDY J.
Owner UNIV LAVAL
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