188results about "Embryonic cells" patented technology

A sequential cell culture system and methods for the in vitro generation of human spermatozoa from differentiating spermatogonia (dSPGs) and spermatogonial stem cells (SSCs) is provided. The instant disclosure encompasses the discovery and optimization of distinct, stage-specific culture media and conditions that enable, for the first time, the complete progression of human spermatogenesis outside the body. The process includes: (1) inducing entry of dSPGs and SSCs into meiosis; (2) supporting meiotic progression and completion to round spermatids; and (3) promoting spermiogenesis and the formation of elongated, spermatozoa. The invention further provides compositions, systems, and protocols for each stage, as well as markers and methods for identifying successful progression through each developmental transition. This system enables, for the first time, the complete in vitro recapitulation of human spermatogenesis, providing a platform for research, infertility treatment, and assisted reproductive technologies.

The present invention provides a cardiac tissue model comprising cardiac tissue having at least one lumen or central cavity, wherein the cardiac tissue model comprises at least two different cardiac tissues selected from left ventricular tissue, right ventricular tissue, atrial tissue, outflow tract tissue, atrioventricular canal tissue, sinoatrial node tissue, and atrioventricular node tissue, the central cavity can be shared by at least two different cardiac tissues, and / or at least two different cardiac tissues comprise the ability to propagate calcium signaling connections and / or tissue contractions, a method of generating such a tissue model, and the use of the tissue model for screening purposes.

Described are small molecule cocktails that enable de novo derivation and long-term culture of bovine extraembryonic endoderm cells (bXENs). Methods of using the small molecule cocktails to generate bXENs are also described. Methods of using the bXENs to form blastocyst models or to maintain the stemness of bovine ESCs and prevent them from differentiation are also described.

The invention encompasses methods for increasing genetic progress in livestock, and for genetic dissemination, including the use of amniocentesis to obtain fetal amniocytes for use in genomic evaluation and cloning.

This application relates to methods of cryopreserving cells for modeling a human brain, a human blood-brain barrier, or a human microvasculature. Also provided are assays based on an induced blood-brain barrier (iBBB), a multicellular integrated human brain tissue (miBrain), "just add neurons" (JANs), or an induced minimal microvascular cell combo (miVasC).

The present disclosure provides a method for promoting in vitro development of mammalian embryos, comprising the step of adding alpha-ketoglutarate in the embryo development fluid at 90-180h after in vitro fertilization. Compared with the method of adding throughout the whole embryo development, the present disclosure finds that the promotion of embryo development efficiency is more significant when alpha-ketoglutarate is added after 90 hours after fertilization (90 hours to 180 hours after fertilization).

Methods are provided for generating pre-oligodendrocyte precursor cells (pre-OPCs), oligodendrocyte precursor cells (OPCs), and pre-myelinating oligodendrocytes (pre-OLs) from human pluripotent stem cells using chemically defined culture media, which allow for the generation of pre-OPCs in as little as 3 days, SOX10+ OLIG2+ NKX2-2+ OPCs in as little as 12 days, and CD9+ A2B5+ O4+ CNPase+ pre-OLs in as little as 18 days. Two alternative culture protocols for generating OPCs are provided. Culture media, isolated cell populations, and kits are also provided.

The present invention relates to in vitro methods for production of testis cells (e.g., Sertoli and / or Leydig cells) and related organoids. The testis cells and testis like organoids may be used for therapeutic purposes including facilitation of the production of spermatogonia from pro-spermatogonia stem cells.

The present invention provides a new site for transplantation. Specifically, the present invention relates to a method for transplanting cells or tissues, particularly pancreatic islets, into the sub-rectus sheath site. The present invention also relates to a method for treating diabetes by transplanting pancreatic islets, particularly hPSC pancreatic islets, into a sub-rectus sheath position.

To provide a method for culturing avian embryos outside the eggshell that can reliably achieve high embryo survival rates and low embryonic developmental abnormality rates, and that is relatively easy to perform, as well as a method for evaluating the toxicity of test substances using such a culture method. [Solution] The embryo and yolk are removed from the fertilized bird egg and transferred together with avian aqueous oocyte white into a roughly cylindrical well in a culture vessel. The opening of the well, which is filled with avian aqueous oocyte white, is then covered with a porous membrane, and the avian embryo is cultured by turning it over. Preferably, the cultured avian embryo is further cultured in a roughly cylindrical well in which a porous membrane is provided along the inner circumference, and the amount of avian aqueous oocyte white is adjusted so that there is a space between the well and the avian yolk below the opening.

The present disclosure belongs to the field of cell engineering, and particularly relates to a soft scaffold suspension self-assembly system for alveolar organoid culture and application thereof. The present disclosure provides a soft scaffold suspension self-assembly system for alveolar organoid culture, which comprises a low-adsorption culture plate for containing a differentiation culture medium, and a Matrigel added into the differentiation culture medium for providing a polarity signal and a growth factor and maintaining a suspension culture environment, wherein the Matrigel and the differentiation culture medium constitute a differentiation culture system, and the volume ratio of the Matrigel to the differentiation culture system is (1-2):100. The present disclosure also provides a culture method for inducing and differentiating an alveolar organoid from human embryonic stem cells, which is based on the soft scaffold suspension self-assembly system and successfully induces and differentiates human pluripotent stem cells into a plurality of key cell types including proximal airway epithelial cells (such as basal cells and goblet cells) and distal alveolar epithelial cells (including functional type 1 and type 2 alveolar cells) within 5 weeks.

The present invention relates to a cell population of spiral ganglion neural progenitors (SGNPs) and methods how to isolate these cells from an in-vitro mixed cell population. The cell population expresses one or more markers, wherein the one or more marker is CXCR4, SUSD2, LPAR3, EPHA5, CHRNA3, REEP1, NPFFR2, SORCS3, NFASC, GABRB3, KCNB2, GRM8, DLL3, and / or KCNH8.

A method of deriving and maintaining Salmonidae stem cells is provided, the method comprising deriving Salmonidae cells at the eyed or blastula stages of salmonid or salmonoid ova development, so as to produce cells with use in the food industry.

The present invention provides a mixing system and method for mixing stem cells, stem cells and nerve cells, stem cells and immune cells, or stem cells, nerve cells and immune cells with pure water or physiological saline. [Solution] The present invention relates to a mixing system (100) for mixing stem cells, stem cells and nerve cells, stem cells and immune cells, or stem cells, nerve cells and immune cells with pure water or physiological saline, and comprises a pressurizing device (2: first pressurizing device), a pressure drop mechanism (4: nozzle, orifice, etc.), a cooling device (3), and a filter (5) for removing nuclei and mitochondria.

The application belongs to the technical field of cell culture and organoid, and particularly relates to a culture medium and a culture method for inducing primitive pluripotent stem cells into amnion-like cells. The application provides a culture medium for inducing primitive pluripotent stem cells into amnion-like cells, and through accurate regulation of key signal pathways such as TGF-beta, Wnt and FGF / ERK, efficient and specific directional differentiation of primitive pluripotent stem cells into amnion-like cells can be achieved. The application has the advantages of clear composition and good repeatability, avoids the use of uncertain components such as serum, and provides a reliable tool for stably obtaining high-quality amnion-like cells. The cells produced thereby are an ideal starting point for constructing amnion organoids and isolating amnion mesenchymal stem cells, and lay an important technical foundation for embryonic development research, disease modeling and regenerative medicine applications.

The present application relates to the field of animal embryo engineering technology, and provides a method for improving the development ability of bovine IVP embryos. In the production of bovine in vitro embryos, by adding PAPPA, ergothioneine and selenocysteine to the bovine oocyte in vitro maturation culture solution, early embryo culture solution, late embryo culture solution, freezing solution and thawing solution, the in vitro maturation performance of oocytes and the development performance and vitality of the subsequent obtained embryos can be effectively improved. Studies have shown that after adding the above additives, the maturation rate of oocytes, the cleavage rate, the blastocyst rate of embryos, and the freezing survival rate, the hatching rate and the ATP content of embryos after freezing preservation are significantly improved, which has important application value.

The application discloses a method for differentiating stem cells into caudal serotonergic neurons, a complete culture medium and application. Specifically, a new culture medium is formed by adding small molecule compounds, so that human pluripotent stem cells are gradually induced to obtain hindbrain neural stem cells, ventral hindbrain caudal neural stem cells, serotonergic precursor cells and caudal serotonergic neurons of the hindbrain. The culture method is simple, and can efficiently obtain mature serotonergic neurons of specific regions, thereby providing an effective cell model for research on diseases related to the serotonergic system.

An apparatus for observation of biological activity in a sample and a method for quantifying cancer intravasation events in a biological sample includes a microfluidic device having a plurality of fluidic channel, including a center channel adjacent to a media channel defining a perfusable partition therebetween; wherein the center channel is arranged to facilitate mesenchymal transition (EMT) in biological cells housed within the center channel induced by a reagent supplied to the media channels.

This invention provides a human embryonic stem cell expansion culture medium, comprising DMEM / F12 medium, ITS-A, HEPES, magnesium ascorbate phosphate, non-essential amino acids, bFGF, FGF2-G3, GlutaMAX, LY-333531, gentamicin sulfate, Y27632, and CHIR99021. Y-27632 inhibits pluripotent stem cell apoptosis, improves adhesion efficiency, maintains pluripotency and colony formation ability; CHIR99021 enhances the self-renewal of pluripotent stem cells and inhibits their differentiation; LY-333531 inhibits spontaneous differentiation of pluripotent stem cells; and gentamicin sulfate reduces the risk of contamination during culture. The culture medium provided by this invention is serum-free, free of allogeneic substances, has a simple composition, and offers excellent expansion speed, effectively maintaining long-term stable passage of human embryonic stem cells, providing stable technical support for scientific research.

This invention provides a method for promoting the differentiation of induced pluripotent stem cells (iPSCs) into three germ layers and its application in differentiation potential detection, relating to the field of biotechnology. The method includes seeding iPSCs into a culture medium containing 5 μM~20 μM E-cadherin activator 1, shaking culture to form embryoids, adding differentiation medium for differentiation culture, obtaining embryoids with activated three germ layer differentiation potential; seeding the embryoids with activated three germ layer differentiation potential into a container coated with a cell adhesion matrix, adding differentiation medium for further differentiation culture, inducing differentiation into three germ layer cells. This invention solves the technical problems in existing technologies where induction of iPSC differentiation into three germ layers via the embryoid pathway results in poor embryoid formation, poor embryoid state, poor long-term maintenance, and poor adhesion after embryoid transfer, leading to poor endoderm differentiation and migration abilities.

The present invention relates to a method for inducing the differentiation of ventral midbrain NSCs into neurons, which comprises contacting a cell population containing ventral midbrain NSCs with an inhibitor of MEK signaling and an inhibitor of NOTCH signaling, wherein the ventral midbrain NSCs co-express the markers FOXA2, LMX1A, EN1, OTX2, and SOX2.

The present disclosure involves the use of the reprogramming factor PHF7, and optionally further using TBX5, MEF2C, MGATA4, HAND2, MYCD, and / or phosphorylated AKT1 to reprogram adult non-cardiomyocytes, such as cardiac fibroblasts into cardiomyocytes, both in vitro and in vivo in adult human and murine models of myocardial infarction and / or heart failure. Such methods find particular use in the treatment of patients' post-myocardial infarction and / or heart failure to prevent, limit, or reverse scarring and to promote myocardial repair.

The current disclosure provides methods and compositions that provide for the insertion of transgenes in stem or progenitor cells without the deleterious effects to T cell differentiation by in vitro T cell differentiation methods. To accomplish this, the inventors have discovered that expression of the transgenes under the control of promoter regions, such as CD8a or CD8b, allows for the coordinated expression pattern that provides for both: 1) high expression of the transgene in mature T cells and 2) a coordinated level of expression of the transgene throughout the in vitro differentiation method that allows for the production of a population of mature T cells.

The present invention provides a method for producing a small intestine epithelial cell or a small intestine tissue from a midgut cell, the method comprising: (1) a step of culturing a midgut cell in a medium containing a Wnt agonist, a BMP inhibitor, and a growth factor to obtain an intestinal progenitor cell; and (2) a step of culturing the intestinal progenitor cell obtained in step (1) in a medium containing a growth factor, an adenylate cyclase activator, an MEK inhibitor, a DNA methylation inhibitor, a TGF-β inhibitor, a GSK3β inhibitor, and a γ-secretase inhibitor to obtain a small intestine epithelial cell or a small intestine tissue.

The present disclosure relates to deoxyribonucleic acid (DNA) editing agents, and their use in preparing genetically modified cells and birds. The present disclosure further relates to fertile genetically modified avians and genetically modified avian primordial germ cells (PGCs) for producing sterile genetically modified avians (birds) that can serve as surrogate hosts for donor PGCs. The present disclosure further relates to methods for producing fertile avian strains that can produce a population of embryos and offspring, in both sexes, all of which are sterile and viable, and further relates to their subsequent use as sterile surrogate hosts for donor PGCs.