135 results about "Surface marker" patented technology

The invention discloses a method for measuring an attitude angle of a free throwing object in a wind tunnel test through a binocular system, and belongs to the field of aerodynamics tests based on a photogrammetry technology. Comprising the steps that a binocular system is arranged in a wind tunnel, and internal and external parameters of the binocular system are calibrated through a cross scale; reconstructing the surface mark points of the calibration plate by photogrammetry outside the wind tunnel and establishing a local coordinate system; placing a calibration plate in the wind tunnel, wherein each vector of a local coordinate system is parallel or vertical to an incoming vector, and calibrating a wind tunnel coordinate system by adopting a resection method; carrying out photogrammetry and initial pose installation on mark points on the surface of a thrown object; acquiring each state image of the thrown object and reconstructing the three-dimensional coordinates of the surface mark points; taking the attitude angle of the thrown object as 0 degree as a reference state, and comparing the rigid body transformation relationship between the blowing state point cloud and the reference state point cloud to obtain attitude angle data. Alignment of a wind tunnel coordinate system and a binocular system coordinate system can be achieved, limitation of the appearance of the thrown object is avoided, and attitude angle data of the thrown object are calculated in a non-contact and high-precision mode.

The invention relates to the technical field of cell preparation quality evaluation, in particular to a cell preparation whole-process quality intelligent evaluation system and a method thereof, and the system comprises a data acquisition module, a preprocessing module, a mining module, a modeling module, a report automatic generation module and a data storage module. The modeling module is used for constructing a cell product index evaluation system and a product traceability model and comprises a cell culture state evaluation model and an environment monitoring analysis module, and the environment monitoring analysis module is used for carrying out traceability analysis by using a time sequence convolutional neural network; the automatic report generation module intelligently evaluates the quality of the whole process and generates a report, the data storage module manages data and model storage, sharing and calling, the system constructs a cell quality evaluation system based on multi-dimensional data, integrates multiple dimensional indexes such as morphology, surface markers and functionality, realizes comprehensive evaluation, and improves the evaluation efficiency. And the one-sidedness of single index evaluation is avoided.

The invention discloses a calibration board and wind tunnel feature point-based binocular system wind tunnel coordinate system calibration method, and belongs to the field of aerodynamic tests. Comprising the following steps: reconstructing surface mark points of a calibration plate by photogrammetry outside a hole and establishing a local coordinate system to obtain coordinates under the local coordinate system; a binocular system is arranged in the hole, and binocular internal and external parameters are calibrated through a special cross calibration ruler; arranging a plurality of circular feature mark points on the surface of the hole wall with overlapped visual fields of the binocular system; the calibration plate is correctly placed according to the geometrical relationship, so that each vector of the local coordinate system is parallel or perpendicular to an incoming vector, and the binocular system reconstructs global points of the calibration plate and registers the global points to achieve alignment of a measurement coordinate system of the binocular system and a wind tunnel coordinate system; reconstructing a three-dimensional coordinate of the fixed point in a wind tunnel coordinate system through a binocular system; and in a subsequent test, adopting resection to realize the recalibration of the wind tunnel coordinate system. According to the invention, high-precision and high-efficiency alignment requirements of a measurement coordinate system and a wind tunnel coordinate system of a binocular system can be met.

The present invention relates to the technical field of stem cell detection, and particularly relates to a determination method for purity and population doubling time of human umbilical cord mesenchymal stem cells (hUC-MSCs). The cultured hUC-MSCs are mixed with the buffer solution to obtain cell suspension; the cell suspension is mixed and incubated with a fluorescein-labeled antibody to obtain an incubated substance; and the incubated substance is centrifuged, and the precipitate obtained is cleaned and then mixed with the buffer solution to obtain a substance to be tested. The present invention analyzes cell surface markers and cell cycles by using a flow cytometer and monoclonal antibodies labeled with different fluoresceins of hUC-MSCs as well as PI staining, and further defines a determination method for purity and population doubling time of hUC-MSCs.

The invention relates to method and kits for highly specific isolation of extracellular vesicles (EVs) by targeting at least two EV surface markers. The invention further relates to methods and kits for analyzing EVs and their contents.

The disclosure relates to method and kits for highly specific detection and quantification of extracellular vesicles (EVs) by targeting at least two EV surface markers using binding reagents. The binding reagents contain hybridisation sequences and primer binding regions, the hybridisation sequences are ligated and amplified using primers. The disclosure further relates to methods and kits for isolating EVs using capture entities, splint entities and staple entities wherein the capture entity comprises a labile linkage.

The utility model relates to the technical field of warning marks, in particular to a danger warning device for chemical safety, which comprises waterproof cloth, wrapping cloth belts are arranged at two ends of the waterproof cloth, aluminum strips are wrapped inside the wrapping cloth belts, a transparent bag is fixed on the surface of the waterproof cloth, warning mark paper is arranged inside the transparent bag, and a warning mark is arranged on the transparent bag. A chemical safety mark is marked on the surface of the warning mark paper sheet; the transparent bag has the beneficial effects that the warning mark paper sheet is arranged in the transparent bag, and the chemical safety mark on the paper sheet is clear and visible. The warning mark paper sheet can be inserted into or taken out of the transparent bag through simple operation, and rapid replacement of warning content is achieved. And meanwhile, the arrangement of the rubber barrier strip effectively prevents the warning mark paper from sliding off, and the durability of the warning effect is guaranteed.

The invention discloses construction of a Guangdong small-ear spotted pig spermatogonial stem cell in-vitro culture system and screening of surface markers, and belongs to the technical field of cell culture.The technical scheme includes that testis are broken firstly, then enzyme digestion, density gradient centrifugal purification and differential adherent purification are conducted, and purified spermatogonial stem cells are obtained; inoculating the obtained spermatogonial stem cells to feeder layer cells and culturing in a culture medium; the culture medium is a DMEM culture medium added with FBS and growth factors; the mass fraction of the FBS in the DMEM culture medium is 8-12%, an enzyme digestion method, a density gradient centrifugation method and a differential adhesion method are combined for application, the Guangdong small-ear spotted pig spermatogonia stem cells with certain purity are obtained, cells enriched and purified in vitro can proliferate massively, and an in-vitro culture system of the Guangdong small-ear spotted pig spermatogonia stem cells is established.

The present disclosure provides methods of producing midbrain dopaminergic neurons (mDAs) and precursors thereof, mDAs and precursors thereof produced thereby, as well as compositions comprising these cells, and their use for the prevention and / or treatment of neurological diseases. The present disclosure also provides methods of isolating mDA and precursors thereof from cell populations using novel surface markers.

This invention discloses a method, apparatus, device, and medium for modifying the date on printed glass trademarks, relating to the field of surface marking on glass products. The method includes the following steps: acquiring a digital pattern of the glass trademark to be modified, and automatically parsing the original date information and its corresponding encoding rules in the pattern using a pre-set date encoding rule library; receiving a target date input, and calculating the graphic representation difference between the original date information and the target date based on the parsed encoding rules, including graphic element areas that need to be removed or added during printing; and visually marking the calculated graphic element areas to be removed using a specific marker color on an overlay layer independent of the original pattern layer, wherein the specific marker color is distinguishable from the ink color used in printing. This invention offers advantages such as high efficiency and accuracy, non-destructive editing, rule-driven operation, flexible adaptation, integrated workflow, and reduced long-term costs.

The present disclosure concerns genetic modification of fibroblasts or fibroblast-derived materials (including of the fibroblasts from which the fibroblast-derived materials are derived). In specific aspects, they are modified such that they may express one or more surface markers that allow targeting to a tissue and / or organ of interest. In some aspects, they are modified such that they may lose one or more surface markers that then allow targeting to a tissue and / or organ of interest. In certain aspects, one or more surface markers are modified that then allows targeting to a tissue and / or organ of interest.

The application discloses a position encoder calibration method and system for printing of a printing substrate, the method comprising: setting a plurality of reference distance intervals divided by a plurality of photoelectric detectors on a conveying path of the printing substrate; in response to a first photoelectric detector being triggered by a front end edge of the printing substrate, clearing the encoder and printing based on the first photoelectric detector; in response to a subsequent photoelectric detector being triggered by the front end edge of the printing substrate, obtaining a cumulative count value of the encoder and calculating a position error of a previous interval; during the printing substrate passing through the current interval, converting the position error into a number of compensation pulses and inserting or skipping a corresponding number of count cells in an expected natural count sequence of the encoder to complete compensation. The application effectively eliminates the accumulated deviation caused by long-distance conveying of a large-size printing substrate by segmenting the measurement error and uniformly distributing the pulses in the next interval for compensation, and realizes high-precision printing without surface marking.

The invention discloses an organoid culture medium, a kit and a method for culturing an organoid, and relates to the technical field of organoid culture. The organoid culture medium provided by the invention can improve the proliferation activity of the organoid, a relatively complete organoid form can be obtained through culture, and the cost of the culture medium is reduced. The tumor cell organoid obtained by adopting the organoid culture medium provided by the invention has a more complete tumor cell surface marker, and the cell damage is smaller.

The invention discloses an application of a natural product WithangatinA in preparation of erythroleukemia induced differentiation treatment drugs, the natural product WithangatinA promotes HEL and K562 cells to be transformed from malignant proliferative cells to normal megakaryocytes by inducing the cell particle size to become larger and significantly increasing multinuclear megakaryocytes and promoting expression of surface labeled molecules differentiated from megakaryocytes at the same time, and the erythroleukemia induced differentiation treatment drugs are obtained. The effect of resisting erythroleukemia is achieved. After the natural product Withangulin A is adopted for treatment, the size and the weight of the spleen of a leukemia mouse can be remarkably reduced, the hematocrit and the platelet number of the mouse are remarkably increased, in addition, treatment of the natural product Withangulin A has no obvious influence on the weight of the mouse, and the safety is high. In-vivo research shows that the medicine can induce megakaryocyte differentiation of erythroleukemia in a mouse body, the effect of resisting erythroleukemia is achieved, and a research basis is provided for development of differentiation treatment medicines of erythroleukemia.

The present application relates to a kind of micro air compressor interface installation and surface marking integrated equipment and method, belong to air compressor auxiliary production equipment field.Structure light detection and transmission module is vertically connected with large rack and fixed on ground, and unqualified product removal module is vertically and closely connected with conveying belt;Micro air compressor transport module is fixed on the large platform of rack shell, and torsion gun movement mechanism is fixed on the large platform, and torsion gun adjustment module is fixedly connected with torsion gun movement mechanism;Interface pickup module is fixed on the frame of shell by pin shaft, surface marking mechanism is fixed on the large platform, and rack shell is fixed on ground;Automatic unloading mechanism is fixed by magnetic attraction device of rack shell and rack shell.The advantages are that the equipment integrates feeding, detection, installation, waste removal and product collection, saves a lot of production time and human resources, reduces the increase rate of misdelivery, miswaste or unqualified products caused by manual detection and installation, and greatly improves production efficiency.

The invention relates to method and kits for highly specific isolation of surface marker displaying agents by targeting at least two surface markers. The invention further relates to methods and kits for analyzing surface marker displaying agents and their contents.

Systems and methods for determining a head pose of a user are described. Images captured by a head-mounted camera worn by the user are received, the images comprising captured markers, wherein each captured marker is one of a plurality of markers on a surface that are in the FOV of the head-mounted camera. Stored markers having a predetermined orientation and corresponding to each captured marker are identified from a database, the stored markers comprising coordinates for its corresponding surface marker. An orientation of the captured marker relative to its corresponding stored marker is calculated and the stored marker is obtained. Based on the stored marker and the orientation, an absolute pose of each marker is determined. Based on the absolute pose of each marker, the head pose of the user relative to the surface is calculated.

This invention discloses a method for the exploration and analysis of karst collapse geological hazards, specifically relating to the field of geological hazard exploration and analysis. The method includes: after collecting current and historical images of a target area on a cloud-based collaborative platform, performing image pairing based on shooting location, shooting orientation, and coverage boundaries; dividing the overlapping areas of each image pair into land tables; and outputting image pairs and land table sequences. For land tables with the same sequence number in each image pair, extracting the boundaries of open water, wet soil, bare soil, vegetation, and shadow, and writing a single land surface marker based on the first digit of the enclosed area. This invention addresses the problem in existing technologies where inconsistent surface conditions between different exploration images are easily misjudged as collapse evolution changes by performing image pairing, land table division, single land surface marker generation, non-comparable grid re-sampling and backfilling, and comparable grid segment change determination.

The present invention relates to a method for generating an enriched population of early cortical neural stem cells (NSCs) or a subpopulation thereof, the method comprising: isolating cells that are positive for the cell surface marker protogenin (PRTG) from an initial population of neural progenitor cells, wherein said isolating is conducted at a time point between about day 4 and about day 12, preferably on day 5, after initiation of neural induction, thereby obtaining an enriched population of early cortical neural stem cells (NSCs); and optionally re- culturing said enriched population of early cortical neural stem cells (NSCs) in a neural induction medium or other culture medium, wherein said re-culturing preferably produces progeny of said early cortical neural stem cells (NSCs).

The invention provides an organoid for simulating a dormancy and recurrence process of breast cancer cells as well as a construction method and application of the organoid. The construction method comprises the following steps: culturing breast cancer cells, and carrying out flow sorting on surface markers CD44 and ITGB4 to obtain an ITGB4 low-expression (ITGB4low) cell population; packaging a lentiviral vector, infecting a cell population with low expression of ITGB4, and carrying out flow sorting to obtain a stably transfected cell population; and carrying out induction treatment on the stably transfected cell population and culturing to obtain the organoid. The organoid provided by the invention provides powerful support for prevention and / or treatment of metastatic breast cancer, and shows extraordinary application prospects in accurate and efficient screening of drugs for targeting dormancy-recurrence process.

The present invention relates to a cell population of spiral ganglion neural progenitors (SGNPs) and methods how to isolate these cells from an in-vitro mixed cell population. The cell population expresses one or more markers, wherein the one or more marker is CXCR4, SUSD2, LPAR3, EPHA5, CHRNA3, REEP1, NPFFR2, SORCS3, NFASC, GABRB3, KCNB2, GRM8, DLL3, and / or KCNH8.

The disclosure relates to methods, compositions, and kits for detecting and analyzing surface marker displaying agents (SMDAs). The disclosure further relates to methods for detecting, diagnosing, monitoring response to treatment or recurrence, or predicting overall survival, progression-free survival or duration of response in a subject suspected of having colorectal cancer (CRC) comprising performing one or more assays on at least one biological sample from the subject to detect one or more specific populations of EVs.

The invention discloses an exosome detection method based on double-hairpin nucleic acid probe assembly, and belongs to the technical field of exosome detection. The exosome detection method comprises the following steps: step 1, constructing a multi-element hairpin probe H1; step 2, constructing a multi-element hairpin probe H2; 3, preparing a probe; 4, multi-element signal probes are assembled, in the step 4, three groups of H1 probes and H2 probes are arranged in total, the proportion of the first group of H1 probes and 400 nM H2 probes is 80 nM, the proportion of the second group of H1 probes and 300 nM H2 probes is 80 nM, and the proportion of the third group of H1 probes and 350 nM H2 probes is 80 nM; according to the exosome detection method based on double-hairpin nucleic acid probe assembly, parallel detection of different exosome surface markers can be achieved by constructing multiple hairpin probes H1 and H2, and meanwhile the requirements for high specificity and stability are met.

A toy vehicle is disclosed that integrates customizable surface markings, removable accessories, and functional storage for drawing instruments. The vehicle includes a main body formed of a smooth, erasable substrate configured to receive and release markings from marking instruments. The marking instruments are dual-purpose, allowing users to draw directly on designated surface regions of the vehicle and to be stowed in integrated compartments that visually mimic functional automotive elements such as headlights, taillights, and exhaust ports. The vehicle further includes removable charms and stylized figures attachable to predefined locations. Internal storage compartments retain accessories when not in use. An embedded drive assembly, power system, and remote-control interface enable electronic actuation, while integrated decorative stickers and modular mounting zones allow for extensive personalization.

The invention discloses application of cell surface markers in quality evaluation of umbilical cord mesenchymal stem cells and application of a kit for detecting the cell surface markers in quality evaluation of the umbilical cord mesenchymal stem cells. The cell surface markers are CD142, CD146, CD106 and CD362. The invention also discloses a quality evaluation method of the umbilical cord mesenchymal stem cells. The method comprises the following steps: detecting the expression condition of the cell surface markers of the cells to be detected, and evaluating the cell quality according to the expression result, the cell surface markers are CD142, CD146, CD106 and CD362, and the cell surface markers are used for detecting the cell surface markers. According to the method, accurate detection and systematic analysis of specific markers of different generations of multiple umbilical cord samples are realized, an influence rule of passage and sample individuals on marker expression is defined, and an established cell characteristic evaluation system can accurately judge the quality and functional potential of the umbilical cord mesenchymal stem cells; the scientific basis is provided for the application of the umbilical cord mesenchymal stem cells.

The invention relates to a miniature air compressor interface mounting and surface marking integrated device and method, and belongs to the field of air compressor auxiliary production equipment. The structured light detecting and conveying module is vertically butted with the large rack and is fixed on the ground; the unqualified product removing module is vertically clung to and butted with the conveying belt; the miniature air compressor conveying module is fixed to a large platform of the machine frame shell, the torsion gun movement mechanism is fixed to the large platform, and the torsion gun adjusting module is fixedly connected with the torsion gun movement mechanism. The interface pickup module is fixed on a frame body of the shell through a pin shaft, the surface marking mechanism is fixed on a large platform, and the frame shell is fixed on the ground; the automatic discharging mechanism is fixed to the machine frame shell through a magnetic attraction device of the machine frame shell. The device has the advantages that the device integrates feeding, detecting, installing, waste removing and finished product collecting, a large amount of manpower resources of production time are saved, the mistaken collection and waste or the increase rate of unqualified products caused by manual detecting and installing are reduced, and the production efficiency is greatly improved.

A vision-enabled fluid flow rate detection system is disclosed. The system includes an image sensing device that images an infusion container supplying a fluid to an infusion pump and a pattern of markings associated with a surface of the container, and identifies a visual difference in the pattern from a default state of the pattern to determine a volume of fluid infused from the infusion container, and used to calculate a volume of fluid in the container and a flow rate of the fluid. When the amount of fluid, as determined by the vision system, differs from the amount reported by the pump, the pump's motor may be adjusted to correct the expected volume or flow rate, as needed. When a severe infusion inaccuracy is detected, an alarm or other indication may be provided, or the infusion terminated.

The invention relates to the technical field of biomedicine, in particular to a screening method for induced pluripotent stem cell cloning. The method comprises the following steps: before subsequent operation of cloning, enabling cells to be in a pretreatment culture condition containing vitamin C; performing surface marker detection on the pre-treated suspected induced pluripotent stem cell clones, and performing spatial positioning according to the surface marker detection; performing chelating agent warm dissociation on the localized clones to separate the target clones; the clones are transferred to a new culture condition and cultured in the presence of an Rho kinase inhibitor. Vitamin C is introduced for pretreatment before a traditional cloning and screening process, so that the induced pluripotent stem cells obtain higher morphological integrity and stress tolerance before being subjected to high stress steps such as dyeing, positioning and dissociation.

The invention relates to the technical field of preparation of composite scaffolds, in particular to a preparation method of a novel photosensitive injectable CMCSMA composite bioactive hydrogel scaffold loaded with urine-derived stem cell subpopulation exosomes, and the preparation method comprises the following steps: S1, preparing reagents and instruments; s2, extracting and culturing urine-derived stem cells; s3, carrying out flow cytometry identification and subgroup sorting on the urine-derived stem cells, wherein the subgroup sorting is based on CD133 and CD271 double-positive cell surface markers; s4, extracting and storing the exosome; s5, synthesizing methacrylated carboxymethyl chitosan; and S6, mixing the extracted exosome with a methacrylated carboxymethyl chitosan solution, so as to prepare the CMCSMA-sExos composite bioactive hydrogel scaffold. According to the invention, the exosome is loaded by the methacrylated carboxymethyl chitosan hydrogel, so that slow release and targeted delivery of the exosome are realized, and the in-vivo action time of the exosome is prolonged.