257 results about "Surface marker" patented technology

The invention discloses a method for measuring an attitude angle of a free throwing object in a wind tunnel test through a binocular system, and belongs to the field of aerodynamics tests based on a photogrammetry technology. Comprising the steps that a binocular system is arranged in a wind tunnel, and internal and external parameters of the binocular system are calibrated through a cross scale; reconstructing the surface mark points of the calibration plate by photogrammetry outside the wind tunnel and establishing a local coordinate system; placing a calibration plate in the wind tunnel, wherein each vector of a local coordinate system is parallel or vertical to an incoming vector, and calibrating a wind tunnel coordinate system by adopting a resection method; carrying out photogrammetry and initial pose installation on mark points on the surface of a thrown object; acquiring each state image of the thrown object and reconstructing the three-dimensional coordinates of the surface mark points; taking the attitude angle of the thrown object as 0 degree as a reference state, and comparing the rigid body transformation relationship between the blowing state point cloud and the reference state point cloud to obtain attitude angle data. Alignment of a wind tunnel coordinate system and a binocular system coordinate system can be achieved, limitation of the appearance of the thrown object is avoided, and attitude angle data of the thrown object are calculated in a non-contact and high-precision mode.

The invention relates to method and kits for highly specific isolation of extracellular vesicles (EVs) by targeting at least two EV surface markers. The invention further relates to methods and kits for analyzing EVs and their contents.

The invention relates to the technical field of cell preparation quality evaluation, in particular to a cell preparation whole-process quality intelligent evaluation system and a method thereof, and the system comprises a data acquisition module, a preprocessing module, a mining module, a modeling module, a report automatic generation module and a data storage module. The modeling module is used for constructing a cell product index evaluation system and a product traceability model and comprises a cell culture state evaluation model and an environment monitoring analysis module, and the environment monitoring analysis module is used for carrying out traceability analysis by using a time sequence convolutional neural network; the automatic report generation module intelligently evaluates the quality of the whole process and generates a report, the data storage module manages data and model storage, sharing and calling, the system constructs a cell quality evaluation system based on multi-dimensional data, integrates multiple dimensional indexes such as morphology, surface markers and functionality, realizes comprehensive evaluation, and improves the evaluation efficiency. And the one-sidedness of single index evaluation is avoided.

In various examples, feature identification using language models for autonomous and semi-autonomous systems and applications is described herein. Systems and methods described herein may use a language model(s) to determine information associated with features, such as surface markings, within an environment. For example, sensor data may be used to generate one or more images or other sensor data representations corresponding to an environment. The image(s) may then be processed to generate input data (e.g., input tokens) that is applied to the language model(s). Based at least on processing the input data, the language model(s) may be trained to output data (e.g., output tokens) representing information associated with one or more features. Additionally, the output data may be used to determine the information associated the feature(s) within the environment, where the information may then be used to update a map and / or navigate one or more machines within the environment.

Described embodiments generally relate to a method for estimating a surface normal from a plurality of images of a surface. The method comprises obtaining a first image depicting at least a portion of the surface and obtaining a second image depicting at least the portion of the surface. The method further comprises determining a homography matrix based on an initial estimated surface normal of the portion of the surface; selecting a first pixel from the first image and determining, by homography transformation, a second pixel from the second image; determining, by applying a trained neural network to at least the first image and the second image; determining a residual between the features of the first pixel and the features of the second pixel; and adjusting the estimated surface normal based on the residual.

The invention relates to the technical field of stem cell biology, and discloses a stem cell quality evaluation system and method, and the system comprises a data collection module which collects local environment data and image data of stem cells in a preset range; the morphological recognition module is used for obtaining cellular morphological data of all stem cells in a preset range through a cellular morphological recognition model; the graph structure data construction module is used for constructing graph structure data; the score marking module is used for measuring the multiplication capacity, the genetic stability capacity and the surface marker expression capacity of the stem cells after the preset duration, and obtaining the quality score of the stem cells through manual marking; the model training module is used for training a stem cell quality scoring model through an optimization algorithm; according to the method, the cellular morphology data of the stem cells are extracted through the multi-branch network of the cellular morphology recognition model, and the interaction among the stem cells is comprehensively considered through the stem cell quality scoring model, so that the accuracy of stem cell quality evaluation is improved.

The invention discloses a typing analysis method for extracellular vesicles secreted by tumor cells and immune cells, which comprises the following steps: designing a PD-L1 protein recognition aptamer PD-L1-L, an EpCAM protein recognition aptamer EpCAM-L and a molecular beacon according to different expression levels of surface markers of the extracellular vesicles (PD-L1 + and EpCAM +) derived from the tumor cells and the extracellular vesicles (PD-L1 + and EpCAM-) derived from the immune cells; when the DNA probe recognizes the extracellular vesicles, fluorescence of the Cy5-PD-L1-L aptamer of the tumor cell source extracellular vesicles is quenched, a fluorescence signal of the Connector is recovered, and the immune cell source extracellular vesicles do not respond, so that the two extracellular vesicles are subjected to typing analysis. The method has the advantages that the extracellular vesicles of different cell sources can be subjected to typing analysis by using a strategy based on double aptamers and double switches, and false positive signals caused by single aptamer recognition are avoided.

The invention discloses a calibration board and wind tunnel feature point-based binocular system wind tunnel coordinate system calibration method, and belongs to the field of aerodynamic tests. Comprising the following steps: reconstructing surface mark points of a calibration plate by photogrammetry outside a hole and establishing a local coordinate system to obtain coordinates under the local coordinate system; a binocular system is arranged in the hole, and binocular internal and external parameters are calibrated through a special cross calibration ruler; arranging a plurality of circular feature mark points on the surface of the hole wall with overlapped visual fields of the binocular system; the calibration plate is correctly placed according to the geometrical relationship, so that each vector of the local coordinate system is parallel or perpendicular to an incoming vector, and the binocular system reconstructs global points of the calibration plate and registers the global points to achieve alignment of a measurement coordinate system of the binocular system and a wind tunnel coordinate system; reconstructing a three-dimensional coordinate of the fixed point in a wind tunnel coordinate system through a binocular system; and in a subsequent test, adopting resection to realize the recalibration of the wind tunnel coordinate system. According to the invention, high-precision and high-efficiency alignment requirements of a measurement coordinate system and a wind tunnel coordinate system of a binocular system can be met.

The invention belongs to the technical field of immunology, and particularly relates to an anti-mandarin fish immune globulin D (IgD) monoclonal antibody as well as a preparation method and application thereof. The invention provides a hybridoma cell strain 5C7 capable of secreting an anti-fish IgD monoclonal antibody or an antigen binding fragment thereof for the first time, the hybridoma cell strain 5C7 is preserved in China Center for Type Culture Collection on January 16, 2025, and the preservation number is CCTCC NO: C202550. The anti-mandarin fish IgD monoclonal antibody or the antigen binding fragment thereof can be subjected to specific reaction with mandarin fish secreting type IgD protein and B cell surface film type IgD, can be applied to detection of IgD in a mandarin fish serum sample, and can also be applied to detection of IgD + B cell surface markers.

The invention relates to the technical field of energy exploration analysis, in particular to a conjoint analysis method for organic matter types and micropore development characteristics in rocks, which comprises the following steps: step 1, drying a blocky or granular rock sample, and performing cold inlaying by using epoxy resin mixed with conductive powder to prepare a cylindrical sample; 2, after the cylindrical sample is demolded, first-time mechanical polishing is carried out; 3, marking the surface of the sample by using an etching objective lens when the sample is observed by a microscope; 4, performing secondary mechanical polishing and argon ion polishing on the sample obtained in the step 3; and 5, carrying out rapid positioning observation by using the scanning electron microscope navigation photo and the marked sample photo. The mark etching method is applied to organic matter microscopic observation marking, after the secondary treatment process is completed, various scanning electron microscope analysis can be carried out on the sample, and multi-scale and multi-characteristic analysis technology combination from the micron level to the nanometer level is achieved.

The invention provides a method for generating platelet-like particles based on MEG-01 cells and evaluating functions of the platelet-like particles, and belongs to the technical field of cell biology. The invention provides a method for generating PLPs by inducing MEG-01 cells. According to the method, the MEG-01 cells are differentiated into PLPs with platelet characteristics by utilizing a PMA inducer. The PLPs disclosed by the invention has characteristic surface marker expression consistent with that of natural platelets, can respond to stimulation of an activator ADP, and shows biological functional characteristics of the platelets. The method for generating PLPs is simple and economical, experimental conditions are highly controllable, and an economical, efficient and repeatable in-vitro model is provided for functional research related to platelets.

The invention relates to a deep learning-based aircraft surface mark point coarse positioning method and a medium, and relates to the technical field of aviation. According to the scheme, the surface image information of the aircraft is collected, the image information is preprocessed and then input into the mark point coarse positioning neural network for mark point recognition, feature extraction and residual processing are carried out through the backbone network to obtain four feature maps, and then the four feature maps serve as input of the neck network; and after feature fusion processing of the feature pyramid network, a mark point identification feature map is obtained through detection of a detection head. And finally, based on the final detection result of the mark point, carrying out coarse positioning on the mark point. Due to the fact that feature extraction is carried out by combining cavity convolution of multiple expansion rates, horizontal edge perception convolution processing and vertical edge perception convolution processing, space information and edge information can be integrated in feature extraction, and the phenomena of false detection and missing detection of mark points in aircraft surface mark point coarse positioning based on deep learning are reduced.

Disclosed herein are methods for isolation and expansion of umbilical cord-derived mesenchymal stem cells (UC-MSCs) having one or more cell surface markers, and compositions comprising such UC-MSCs. Also disclosed herein are further processing methods for use in connection with the UC-MSCs to isolate extracellular vesicles (EVs) therefrom. Also disclosed herein are lyophilized EVs isolated from the UC-MSCs, and adapted for use as a therapeutic product.

The invention relates to the technical field of tissue engineering, and particularly discloses preparation of an exosome and electrostatic spinning composite material for promoting bone regeneration and application of the exosome and electrostatic spinning composite material in bone tissue regeneration. Identifying the multiplication capacity, the surface marker and the multidirectional differentiation capacity of the cells after the cells are separated and cultured, and finally extracting and identifying the exosome; pLLA and gelatin are dissolved in a cosolvent trifluoroethanol, composite fibers are prepared through electrostatic spinning, and a composite fiber membrane is obtained through spinning by adopting a receiver; the exosome is loaded on the surface of the PLLA / gelatin composite fiber membrane. According to the invention, the exosome derived from human mesenchymal stem cells is combined with the membrane material to prepare the exosome capable of stably releasing, the good stability and low immunogenicity of the exosome are exerted, and the effect of accurately promoting local bone regeneration is realized. And defect repair and functional reconstruction are realized.

The invention relates to an IL5RA cell surface marker. A cell tag comprising an extracellular region, a transmembrane region, and optionally an intracellular region is provided. The extracellular region comprises an IL5 receptor alpha (IL5Ra) sequence linked to a transmembrane domain, and the recombinant polypeptide cannot function in signal transduction. The cell tag can be operably linked to a transgene. Expression of the cell tag allows for the identification, detection, selection, and ablation of cells expressing the transgene and the cell tag. In some embodiments, the genetically modified host cell comprises a transgene comprising a polynucleotide encoding a chimeric antigen receptor comprising a ligand binding domain and a polynucleotide encoding a cell tag. Pharmaceutical formulations produced by the methods and methods of using the pharmaceutical formulations are also described.

The invention relates to a preparation method of a three-dimensional atom probe sample, which comprises the following steps: S1, imaging a sample under a scanning electron microscope, and marking an interested area on the surface of the sample; s2, cutting the region to expose the section of the sample; s3, performing ion beam irradiation on the cross section so as to generate bright and dark contrast beneficial to observation on two sides of a crystal boundary in the cross section; s4, according to the position of the contrast, cutting the sample to form a slice sample containing a grain boundary; and S5, annularly cutting the sheet sample to form a needle point sample containing the crystal boundary. According to the preparation method, efficient and accurate positioning is provided, the sample preparation time is effectively shortened, and the sample preparation success rate is increased.

The invention discloses an umbilical cord mesenchymal stem cell serum-free culture medium which is composed of a basic culture medium and an additive, and the basic culture medium is DMEM / F12. The additive is prepared from albumin, transferrin, insulin, a fibroblast growth factor (FGF), an epidermal growth factor (EGF), dexamethasone, glutamine, ascorbic acid, recombinant human III type collagen and recombinant human XVII type collagen. According to the UCMSCs obtained by culturing the umbilical cord mesenchymal stem cells by using the serum-free culture medium, the cellular morphology and surface marker expression are maintained; the umbilical cord mesenchymal stem cell serum-free culture medium has the advantages that the cell proliferation capacity, the monoclonal performance capacity and the three-line differentiation capacity are enhanced, the umbilical cord mesenchymal stem cell serum-free culture medium only contains 12 raw materials, the compounding process is simple, the cost is low, the raw materials of the umbilical cord mesenchymal stem cell serum-free culture medium are clear in quality, and the umbilical cord mesenchymal stem cell serum-free culture medium can be used for culturing clinical-grade UCMSCs.

The present invention relates to the technical field of stem cell detection, and particularly relates to a determination method for purity and population doubling time of human umbilical cord mesenchymal stem cells (hUC-MSCs). The cultured hUC-MSCs are mixed with the buffer solution to obtain cell suspension; the cell suspension is mixed and incubated with a fluorescein-labeled antibody to obtain an incubated substance; and the incubated substance is centrifuged, and the precipitate obtained is cleaned and then mixed with the buffer solution to obtain a substance to be tested. The present invention analyzes cell surface markers and cell cycles by using a flow cytometer and monoclonal antibodies labeled with different fluoresceins of hUC-MSCs as well as PI staining, and further defines a determination method for purity and population doubling time of hUC-MSCs.

The invention relates to method and kits for highly specific isolation of extracellular vesicles (EVs) by targeting at least two EV surface markers. The invention further relates to methods and kits for analyzing EVs and their contents.

The disclosure relates to an adeno-associated virus (AAV) capsid fusion protein comprising a surface marker binding domain (SMBD), in particular, a single-domain antibody (sdAb) or variable immunoglobulin domain of a heavy chain antibody (VHH), which is inserted in the GH2-GH3 loop of the AAV VP3 capsid protein. Also, an AAV vector particle comprising a SMBD inserted into the VP3 AAV capsid protein is contemplated. Further, nucleotide sequences encoding such fusion proteins and vectors including such nucleotide sequences are contemplated. Further encompassed is a recombinant adeno-associated virus (AAV) which comprises said AAV capsid fusion protein. Moreover the disclosure relates to uses of the fusion proteins, nucleic sequences and corresponding AAVs for use introducing a defined nucleic sequence into a target cell or for improving the transduction efficiency of the AAV targeted cells. The disclosure also relates to methods for producing recombinant AAV, in particular a recombinant AAV that comprises an insert of a surface marker binding domain (SMBD) in one type of the VP capsid protein selected from VP1, VP2 and VP3, but not in the other two types of VP capsid proteins.

The disclosure relates to method and kits for highly specific detection and quantification of extracellular vesicles (EVs) by targeting at least two EV surface markers using binding reagents. The binding reagents contain hybridisation sequences and primer binding regions, the hybridisation sequences are ligated and amplified using primers. The disclosure further relates to methods and kits for isolating EVs using capture entities, splint entities and staple entities wherein the capture entity comprises a labile linkage.

The invention relates to a classification marking device and marking method for marine T profile welding defects, the classification marking device comprises a translation device, a lifting device, a lineation device and a mounting platform fixedly mounted with welding equipment, and the lineation device is mounted at the lower part of the lifting device; the lifting device is mounted on the translation device in an inverted hanging manner; the translation device is inversely hung on the mounting platform, and the mounting direction is perpendicular to the moving direction of the welding equipment; and the translation device and the lifting device are connected with the motion controller. The marking device is driven by the welding equipment to move, automatic marking can be achieved, the efficiency is high, and the labor requirement is low; defect classification marking: different marks can be marked on the surface of the T-shaped profile through cooperative work of movement of an automatic translation device and welding equipment, so that classification marking of welding defects is realized; the chalk is pressed on the surface of the T-shaped profile through the elastic element, reliable marking can be achieved, it can be guaranteed that the marking device and the T-shaped profile cannot extrude each other, and equipment safety is guaranteed.

The invention provides an extracellular vesicle marker separation-free detection method based on nanoparticle enhancement, and belongs to the technical field of detection methods, the method comprises the following steps: preparing gold nanoparticles with the surface modified with a first antibody, the first antibody being used for specific recognition of an extracellular vesicle surface marker; determining the particle size distribution of the gold nanoparticles by adopting a dynamic light scattering method, and determining the surface potentials and surface isoelectric points of the gold nanoparticles by adopting a potentiostat; adopting a transmission electron microscope to characterize the morphology of the screened gold nanoparticles; co-modifying polyethylene glycol molecules on the surfaces of the gold nanoparticles to form an anti-interference layer; mixing the gold nanoparticles with uniform morphology with a sample to be detected; adding a second antibody into the mixed system; distinguishing signals of the binding-state second antibody and the free-state second antibody through fluorescence lifetime analysis; preparing an extracellular vesicle standard substance, and obtaining a standard curve and a system response curve; the optimal detection condition is determined by solving the detection system optimization equation set, and separation-free detection is carried out.

The invention relates to a vehicle-mounted refrigerator welding control method, device and equipment and a storage medium, and the method comprises the steps that a welding identification code of a vehicle-mounted refrigerator fixed to a preset welding station is determined, and three-dimensional geometric data and surface mark point information of the vehicle-mounted refrigerator are determined; welding positioning data of a welded junction are determined through a geometric matching algorithm to adjust the pose of a welding gun; the welded junction is welded according to the first welding data, state information of the welded junction is collected in real time, and welding operation is adjusted according to the state information of the welded junction; when the welded junction meets the preset welding quality threshold value, a welding ending instruction is correspondingly generated to adjust the posture of the welding gun, and welding parameters corresponding to the welding operation are bound with the welding identification code to obtain second welding data; and the first welding data are updated according to the second welding data, and the vehicle-mounted refrigerator is moved out of the welding station. High precision, high stability and whole-process traceability of the welding process of the vehicle-mounted refrigerator are achieved, and the welding quality and the production efficiency are remarkably improved.

The invention discloses a lupus nephritis marker and application thereof, and belongs to the technical field of biomarkers. The marker for the lupus nephritis, disclosed by the invention, comprises one or more of CD8, CD38 and PD-1. By detecting specific cell surface markers in patient blood, early diagnosis of LN is assisted in a minimally invasive mode, and based on deep comparative analysis of LN patients and healthy people, the specificity and sensitivity of the biomarkers in LN diagnosis are ensured through screening of the biomarkers. In the aspect of differential diagnosis, a clinician is assisted to distinguish LN from other types of kidney diseases, such as membranous nephropathy (MN) and anti-neutrophil cytoplasm antibody related vasculitis (AAV), by analyzing specific biomarker modes of different nephropathy. The dependence on kidney puncture is reduced, related potential risks and inconvenience are avoided, and meanwhile more accurate and timely diagnosis is provided for an SLE patient.

The utility model relates to the technical field of warning marks, in particular to a danger warning device for chemical safety, which comprises waterproof cloth, wrapping cloth belts are arranged at two ends of the waterproof cloth, aluminum strips are wrapped inside the wrapping cloth belts, a transparent bag is fixed on the surface of the waterproof cloth, warning mark paper is arranged inside the transparent bag, and a warning mark is arranged on the transparent bag. A chemical safety mark is marked on the surface of the warning mark paper sheet; the transparent bag has the beneficial effects that the warning mark paper sheet is arranged in the transparent bag, and the chemical safety mark on the paper sheet is clear and visible. The warning mark paper sheet can be inserted into or taken out of the transparent bag through simple operation, and rapid replacement of warning content is achieved. And meanwhile, the arrangement of the rubber barrier strip effectively prevents the warning mark paper from sliding off, and the durability of the warning effect is guaranteed.

The invention discloses construction of a Guangdong small-ear spotted pig spermatogonial stem cell in-vitro culture system and screening of surface markers, and belongs to the technical field of cell culture.The technical scheme includes that testis are broken firstly, then enzyme digestion, density gradient centrifugal purification and differential adherent purification are conducted, and purified spermatogonial stem cells are obtained; inoculating the obtained spermatogonial stem cells to feeder layer cells and culturing in a culture medium; the culture medium is a DMEM culture medium added with FBS and growth factors; the mass fraction of the FBS in the DMEM culture medium is 8-12%, an enzyme digestion method, a density gradient centrifugation method and a differential adhesion method are combined for application, the Guangdong small-ear spotted pig spermatogonia stem cells with certain purity are obtained, cells enriched and purified in vitro can proliferate massively, and an in-vitro culture system of the Guangdong small-ear spotted pig spermatogonia stem cells is established.

The present disclosure provides methods of producing midbrain dopaminergic neurons (mDAs) and precursors thereof, mDAs and precursors thereof produced thereby, as well as compositions comprising these cells, and their use for the prevention and / or treatment of neurological diseases. The present disclosure also provides methods of isolating mDA and precursors thereof from cell populations using novel surface markers.

This invention discloses a method, apparatus, device, and medium for modifying the date on printed glass trademarks, relating to the field of surface marking on glass products. The method includes the following steps: acquiring a digital pattern of the glass trademark to be modified, and automatically parsing the original date information and its corresponding encoding rules in the pattern using a pre-set date encoding rule library; receiving a target date input, and calculating the graphic representation difference between the original date information and the target date based on the parsed encoding rules, including graphic element areas that need to be removed or added during printing; and visually marking the calculated graphic element areas to be removed using a specific marker color on an overlay layer independent of the original pattern layer, wherein the specific marker color is distinguishable from the ink color used in printing. This invention offers advantages such as high efficiency and accuracy, non-destructive editing, rule-driven operation, flexible adaptation, integrated workflow, and reduced long-term costs.

The present disclosure concerns genetic modification of fibroblasts or fibroblast-derived materials (including of the fibroblasts from which the fibroblast-derived materials are derived). In specific aspects, they are modified such that they may express one or more surface markers that allow targeting to a tissue and / or organ of interest. In some aspects, they are modified such that they may lose one or more surface markers that then allow targeting to a tissue and / or organ of interest. In certain aspects, one or more surface markers are modified that then allows targeting to a tissue and / or organ of interest.