204 results about "Surface marker" patented technology

The invention discloses a method for measuring an attitude angle of a free throwing object in a wind tunnel test through a binocular system, and belongs to the field of aerodynamics tests based on a photogrammetry technology. Comprising the steps that a binocular system is arranged in a wind tunnel, and internal and external parameters of the binocular system are calibrated through a cross scale; reconstructing the surface mark points of the calibration plate by photogrammetry outside the wind tunnel and establishing a local coordinate system; placing a calibration plate in the wind tunnel, wherein each vector of a local coordinate system is parallel or vertical to an incoming vector, and calibrating a wind tunnel coordinate system by adopting a resection method; carrying out photogrammetry and initial pose installation on mark points on the surface of a thrown object; acquiring each state image of the thrown object and reconstructing the three-dimensional coordinates of the surface mark points; taking the attitude angle of the thrown object as 0 degree as a reference state, and comparing the rigid body transformation relationship between the blowing state point cloud and the reference state point cloud to obtain attitude angle data. Alignment of a wind tunnel coordinate system and a binocular system coordinate system can be achieved, limitation of the appearance of the thrown object is avoided, and attitude angle data of the thrown object are calculated in a non-contact and high-precision mode.

The invention relates to the technical field of cell preparation quality evaluation, in particular to a cell preparation whole-process quality intelligent evaluation system and a method thereof, and the system comprises a data acquisition module, a preprocessing module, a mining module, a modeling module, a report automatic generation module and a data storage module. The modeling module is used for constructing a cell product index evaluation system and a product traceability model and comprises a cell culture state evaluation model and an environment monitoring analysis module, and the environment monitoring analysis module is used for carrying out traceability analysis by using a time sequence convolutional neural network; the automatic report generation module intelligently evaluates the quality of the whole process and generates a report, the data storage module manages data and model storage, sharing and calling, the system constructs a cell quality evaluation system based on multi-dimensional data, integrates multiple dimensional indexes such as morphology, surface markers and functionality, realizes comprehensive evaluation, and improves the evaluation efficiency. And the one-sidedness of single index evaluation is avoided.

In various examples, feature identification using language models for autonomous and semi-autonomous systems and applications is described herein. Systems and methods described herein may use a language model(s) to determine information associated with features, such as surface markings, within an environment. For example, sensor data may be used to generate one or more images or other sensor data representations corresponding to an environment. The image(s) may then be processed to generate input data (e.g., input tokens) that is applied to the language model(s). Based at least on processing the input data, the language model(s) may be trained to output data (e.g., output tokens) representing information associated with one or more features. Additionally, the output data may be used to determine the information associated the feature(s) within the environment, where the information may then be used to update a map and / or navigate one or more machines within the environment.

The invention relates to the technical field of stem cell biology, and discloses a stem cell quality evaluation system and method, and the system comprises a data collection module which collects local environment data and image data of stem cells in a preset range; the morphological recognition module is used for obtaining cellular morphological data of all stem cells in a preset range through a cellular morphological recognition model; the graph structure data construction module is used for constructing graph structure data; the score marking module is used for measuring the multiplication capacity, the genetic stability capacity and the surface marker expression capacity of the stem cells after the preset duration, and obtaining the quality score of the stem cells through manual marking; the model training module is used for training a stem cell quality scoring model through an optimization algorithm; according to the method, the cellular morphology data of the stem cells are extracted through the multi-branch network of the cellular morphology recognition model, and the interaction among the stem cells is comprehensively considered through the stem cell quality scoring model, so that the accuracy of stem cell quality evaluation is improved.

The invention discloses a calibration board and wind tunnel feature point-based binocular system wind tunnel coordinate system calibration method, and belongs to the field of aerodynamic tests. Comprising the following steps: reconstructing surface mark points of a calibration plate by photogrammetry outside a hole and establishing a local coordinate system to obtain coordinates under the local coordinate system; a binocular system is arranged in the hole, and binocular internal and external parameters are calibrated through a special cross calibration ruler; arranging a plurality of circular feature mark points on the surface of the hole wall with overlapped visual fields of the binocular system; the calibration plate is correctly placed according to the geometrical relationship, so that each vector of the local coordinate system is parallel or perpendicular to an incoming vector, and the binocular system reconstructs global points of the calibration plate and registers the global points to achieve alignment of a measurement coordinate system of the binocular system and a wind tunnel coordinate system; reconstructing a three-dimensional coordinate of the fixed point in a wind tunnel coordinate system through a binocular system; and in a subsequent test, adopting resection to realize the recalibration of the wind tunnel coordinate system. According to the invention, high-precision and high-efficiency alignment requirements of a measurement coordinate system and a wind tunnel coordinate system of a binocular system can be met.

The invention belongs to the technical field of immunology, and particularly relates to an anti-mandarin fish immune globulin D (IgD) monoclonal antibody as well as a preparation method and application thereof. The invention provides a hybridoma cell strain 5C7 capable of secreting an anti-fish IgD monoclonal antibody or an antigen binding fragment thereof for the first time, the hybridoma cell strain 5C7 is preserved in China Center for Type Culture Collection on January 16, 2025, and the preservation number is CCTCC NO: C202550. The anti-mandarin fish IgD monoclonal antibody or the antigen binding fragment thereof can be subjected to specific reaction with mandarin fish secreting type IgD protein and B cell surface film type IgD, can be applied to detection of IgD in a mandarin fish serum sample, and can also be applied to detection of IgD + B cell surface markers.

The invention relates to a deep learning-based aircraft surface mark point coarse positioning method and a medium, and relates to the technical field of aviation. According to the scheme, the surface image information of the aircraft is collected, the image information is preprocessed and then input into the mark point coarse positioning neural network for mark point recognition, feature extraction and residual processing are carried out through the backbone network to obtain four feature maps, and then the four feature maps serve as input of the neck network; and after feature fusion processing of the feature pyramid network, a mark point identification feature map is obtained through detection of a detection head. And finally, based on the final detection result of the mark point, carrying out coarse positioning on the mark point. Due to the fact that feature extraction is carried out by combining cavity convolution of multiple expansion rates, horizontal edge perception convolution processing and vertical edge perception convolution processing, space information and edge information can be integrated in feature extraction, and the phenomena of false detection and missing detection of mark points in aircraft surface mark point coarse positioning based on deep learning are reduced.

Disclosed herein are methods for isolation and expansion of umbilical cord-derived mesenchymal stem cells (UC-MSCs) having one or more cell surface markers, and compositions comprising such UC-MSCs. Also disclosed herein are further processing methods for use in connection with the UC-MSCs to isolate extracellular vesicles (EVs) therefrom. Also disclosed herein are lyophilized EVs isolated from the UC-MSCs, and adapted for use as a therapeutic product.

The present invention relates to the technical field of stem cell detection, and particularly relates to a determination method for purity and population doubling time of human umbilical cord mesenchymal stem cells (hUC-MSCs). The cultured hUC-MSCs are mixed with the buffer solution to obtain cell suspension; the cell suspension is mixed and incubated with a fluorescein-labeled antibody to obtain an incubated substance; and the incubated substance is centrifuged, and the precipitate obtained is cleaned and then mixed with the buffer solution to obtain a substance to be tested. The present invention analyzes cell surface markers and cell cycles by using a flow cytometer and monoclonal antibodies labeled with different fluoresceins of hUC-MSCs as well as PI staining, and further defines a determination method for purity and population doubling time of hUC-MSCs.

The invention relates to method and kits for highly specific isolation of extracellular vesicles (EVs) by targeting at least two EV surface markers. The invention further relates to methods and kits for analyzing EVs and their contents.

The disclosure relates to method and kits for highly specific detection and quantification of extracellular vesicles (EVs) by targeting at least two EV surface markers using binding reagents. The binding reagents contain hybridisation sequences and primer binding regions, the hybridisation sequences are ligated and amplified using primers. The disclosure further relates to methods and kits for isolating EVs using capture entities, splint entities and staple entities wherein the capture entity comprises a labile linkage.

The invention relates to a classification marking device and marking method for marine T profile welding defects, the classification marking device comprises a translation device, a lifting device, a lineation device and a mounting platform fixedly mounted with welding equipment, and the lineation device is mounted at the lower part of the lifting device; the lifting device is mounted on the translation device in an inverted hanging manner; the translation device is inversely hung on the mounting platform, and the mounting direction is perpendicular to the moving direction of the welding equipment; and the translation device and the lifting device are connected with the motion controller. The marking device is driven by the welding equipment to move, automatic marking can be achieved, the efficiency is high, and the labor requirement is low; defect classification marking: different marks can be marked on the surface of the T-shaped profile through cooperative work of movement of an automatic translation device and welding equipment, so that classification marking of welding defects is realized; the chalk is pressed on the surface of the T-shaped profile through the elastic element, reliable marking can be achieved, it can be guaranteed that the marking device and the T-shaped profile cannot extrude each other, and equipment safety is guaranteed.

The invention provides an extracellular vesicle marker separation-free detection method based on nanoparticle enhancement, and belongs to the technical field of detection methods, the method comprises the following steps: preparing gold nanoparticles with the surface modified with a first antibody, the first antibody being used for specific recognition of an extracellular vesicle surface marker; determining the particle size distribution of the gold nanoparticles by adopting a dynamic light scattering method, and determining the surface potentials and surface isoelectric points of the gold nanoparticles by adopting a potentiostat; adopting a transmission electron microscope to characterize the morphology of the screened gold nanoparticles; co-modifying polyethylene glycol molecules on the surfaces of the gold nanoparticles to form an anti-interference layer; mixing the gold nanoparticles with uniform morphology with a sample to be detected; adding a second antibody into the mixed system; distinguishing signals of the binding-state second antibody and the free-state second antibody through fluorescence lifetime analysis; preparing an extracellular vesicle standard substance, and obtaining a standard curve and a system response curve; the optimal detection condition is determined by solving the detection system optimization equation set, and separation-free detection is carried out.

The invention relates to a vehicle-mounted refrigerator welding control method, device and equipment and a storage medium, and the method comprises the steps that a welding identification code of a vehicle-mounted refrigerator fixed to a preset welding station is determined, and three-dimensional geometric data and surface mark point information of the vehicle-mounted refrigerator are determined; welding positioning data of a welded junction are determined through a geometric matching algorithm to adjust the pose of a welding gun; the welded junction is welded according to the first welding data, state information of the welded junction is collected in real time, and welding operation is adjusted according to the state information of the welded junction; when the welded junction meets the preset welding quality threshold value, a welding ending instruction is correspondingly generated to adjust the posture of the welding gun, and welding parameters corresponding to the welding operation are bound with the welding identification code to obtain second welding data; and the first welding data are updated according to the second welding data, and the vehicle-mounted refrigerator is moved out of the welding station. High precision, high stability and whole-process traceability of the welding process of the vehicle-mounted refrigerator are achieved, and the welding quality and the production efficiency are remarkably improved.

The invention discloses a lupus nephritis marker and application thereof, and belongs to the technical field of biomarkers. The marker for the lupus nephritis, disclosed by the invention, comprises one or more of CD8, CD38 and PD-1. By detecting specific cell surface markers in patient blood, early diagnosis of LN is assisted in a minimally invasive mode, and based on deep comparative analysis of LN patients and healthy people, the specificity and sensitivity of the biomarkers in LN diagnosis are ensured through screening of the biomarkers. In the aspect of differential diagnosis, a clinician is assisted to distinguish LN from other types of kidney diseases, such as membranous nephropathy (MN) and anti-neutrophil cytoplasm antibody related vasculitis (AAV), by analyzing specific biomarker modes of different nephropathy. The dependence on kidney puncture is reduced, related potential risks and inconvenience are avoided, and meanwhile more accurate and timely diagnosis is provided for an SLE patient.

The utility model relates to the technical field of warning marks, in particular to a danger warning device for chemical safety, which comprises waterproof cloth, wrapping cloth belts are arranged at two ends of the waterproof cloth, aluminum strips are wrapped inside the wrapping cloth belts, a transparent bag is fixed on the surface of the waterproof cloth, warning mark paper is arranged inside the transparent bag, and a warning mark is arranged on the transparent bag. A chemical safety mark is marked on the surface of the warning mark paper sheet; the transparent bag has the beneficial effects that the warning mark paper sheet is arranged in the transparent bag, and the chemical safety mark on the paper sheet is clear and visible. The warning mark paper sheet can be inserted into or taken out of the transparent bag through simple operation, and rapid replacement of warning content is achieved. And meanwhile, the arrangement of the rubber barrier strip effectively prevents the warning mark paper from sliding off, and the durability of the warning effect is guaranteed.

The invention discloses construction of a Guangdong small-ear spotted pig spermatogonial stem cell in-vitro culture system and screening of surface markers, and belongs to the technical field of cell culture.The technical scheme includes that testis are broken firstly, then enzyme digestion, density gradient centrifugal purification and differential adherent purification are conducted, and purified spermatogonial stem cells are obtained; inoculating the obtained spermatogonial stem cells to feeder layer cells and culturing in a culture medium; the culture medium is a DMEM culture medium added with FBS and growth factors; the mass fraction of the FBS in the DMEM culture medium is 8-12%, an enzyme digestion method, a density gradient centrifugation method and a differential adhesion method are combined for application, the Guangdong small-ear spotted pig spermatogonia stem cells with certain purity are obtained, cells enriched and purified in vitro can proliferate massively, and an in-vitro culture system of the Guangdong small-ear spotted pig spermatogonia stem cells is established.

The present disclosure provides methods of producing midbrain dopaminergic neurons (mDAs) and precursors thereof, mDAs and precursors thereof produced thereby, as well as compositions comprising these cells, and their use for the prevention and / or treatment of neurological diseases. The present disclosure also provides methods of isolating mDA and precursors thereof from cell populations using novel surface markers.

This invention discloses a method, apparatus, device, and medium for modifying the date on printed glass trademarks, relating to the field of surface marking on glass products. The method includes the following steps: acquiring a digital pattern of the glass trademark to be modified, and automatically parsing the original date information and its corresponding encoding rules in the pattern using a pre-set date encoding rule library; receiving a target date input, and calculating the graphic representation difference between the original date information and the target date based on the parsed encoding rules, including graphic element areas that need to be removed or added during printing; and visually marking the calculated graphic element areas to be removed using a specific marker color on an overlay layer independent of the original pattern layer, wherein the specific marker color is distinguishable from the ink color used in printing. This invention offers advantages such as high efficiency and accuracy, non-destructive editing, rule-driven operation, flexible adaptation, integrated workflow, and reduced long-term costs.

The present disclosure concerns genetic modification of fibroblasts or fibroblast-derived materials (including of the fibroblasts from which the fibroblast-derived materials are derived). In specific aspects, they are modified such that they may express one or more surface markers that allow targeting to a tissue and / or organ of interest. In some aspects, they are modified such that they may lose one or more surface markers that then allow targeting to a tissue and / or organ of interest. In certain aspects, one or more surface markers are modified that then allows targeting to a tissue and / or organ of interest.

The application discloses a position encoder calibration method and system for printing of a printing substrate, the method comprising: setting a plurality of reference distance intervals divided by a plurality of photoelectric detectors on a conveying path of the printing substrate; in response to a first photoelectric detector being triggered by a front end edge of the printing substrate, clearing the encoder and printing based on the first photoelectric detector; in response to a subsequent photoelectric detector being triggered by the front end edge of the printing substrate, obtaining a cumulative count value of the encoder and calculating a position error of a previous interval; during the printing substrate passing through the current interval, converting the position error into a number of compensation pulses and inserting or skipping a corresponding number of count cells in an expected natural count sequence of the encoder to complete compensation. The application effectively eliminates the accumulated deviation caused by long-distance conveying of a large-size printing substrate by segmenting the measurement error and uniformly distributing the pulses in the next interval for compensation, and realizes high-precision printing without surface marking.

The invention relates to a human peripheral blood cell immunotyping antibody combination and method based on flow cytometry. The antibody combination comprises an anti-CD3 antibody, an anti-CD4 antibody, an anti-CD8 antibody, an anti-EGFR antibody, an anti-CD25 antibody, an anti-CD127 antibody, an anti-CD45RA antibody, an anti-CCR7 antibody, an anti-CD27 antibody, an anti-PD-1 antibody, an antiTIGIT antibody and an anti-41BB antibody. The method comprises the following steps: mixing the antibody combination with a sample to be detected, incubating, performing flow cytometry detection to obtain detection data, and further judging the proportion and / or the number of target cell populations. According to the present invention, the optimal cell surface marker antibody combination is designed and obtained, the CAR-T cells in the sample are subjected to immune typing by using the 12-color antibody combination through the flow cytometry, the detection result is rapidly obtained, the CAR-T cell proportion of each phenotype is efficiently identified, and the method has high precision and high stability.

The invention discloses an organoid culture medium, a kit and a method for culturing an organoid, and relates to the technical field of organoid culture. The organoid culture medium provided by the invention can improve the proliferation activity of the organoid, a relatively complete organoid form can be obtained through culture, and the cost of the culture medium is reduced. The tumor cell organoid obtained by adopting the organoid culture medium provided by the invention has a more complete tumor cell surface marker, and the cell damage is smaller.

The invention discloses an application of a natural product WithangatinA in preparation of erythroleukemia induced differentiation treatment drugs, the natural product WithangatinA promotes HEL and K562 cells to be transformed from malignant proliferative cells to normal megakaryocytes by inducing the cell particle size to become larger and significantly increasing multinuclear megakaryocytes and promoting expression of surface labeled molecules differentiated from megakaryocytes at the same time, and the erythroleukemia induced differentiation treatment drugs are obtained. The effect of resisting erythroleukemia is achieved. After the natural product Withangulin A is adopted for treatment, the size and the weight of the spleen of a leukemia mouse can be remarkably reduced, the hematocrit and the platelet number of the mouse are remarkably increased, in addition, treatment of the natural product Withangulin A has no obvious influence on the weight of the mouse, and the safety is high. In-vivo research shows that the medicine can induce megakaryocyte differentiation of erythroleukemia in a mouse body, the effect of resisting erythroleukemia is achieved, and a research basis is provided for development of differentiation treatment medicines of erythroleukemia.

The present application relates to a kind of micro air compressor interface installation and surface marking integrated equipment and method, belong to air compressor auxiliary production equipment field.Structure light detection and transmission module is vertically connected with large rack and fixed on ground, and unqualified product removal module is vertically and closely connected with conveying belt;Micro air compressor transport module is fixed on the large platform of rack shell, and torsion gun movement mechanism is fixed on the large platform, and torsion gun adjustment module is fixedly connected with torsion gun movement mechanism;Interface pickup module is fixed on the frame of shell by pin shaft, surface marking mechanism is fixed on the large platform, and rack shell is fixed on ground;Automatic unloading mechanism is fixed by magnetic attraction device of rack shell and rack shell.The advantages are that the equipment integrates feeding, detection, installation, waste removal and product collection, saves a lot of production time and human resources, reduces the increase rate of misdelivery, miswaste or unqualified products caused by manual detection and installation, and greatly improves production efficiency.

The utility model discloses a tool for measuring a mountain photovoltaic point location, which belongs to the technical field of photovoltaic point location measurement and comprises a measuring tool assembly. A telescopic round steel column; the two lifting sleeves are symmetrically arranged, and each lifting sleeve is longitudinally and slidably arranged on the peripheral side of the corresponding extension type stand column in a sleeving mode; the nylon rope is fixedly connected between the outer walls of the two liftable sleeves, the center point of the pile foundation is positioned at a plurality of middle positions marked on the surface of the nylon rope through plumb lines, and other marks are inserted, so that construction and retesting of a pile machine are facilitated; the rope tensioning adjustment comprises tensioning wheels arranged on the outer sides of the two liftable sleeves respectively, one tensioning wheel descends to press one end of the nylon rope, the other tensioning wheel ascends to jack up the other end of the nylon rope, and the nylon rope is in a straightened and droop-free state. According to the utility model, the tension degree of the nylon rope can be accurately controlled, and the nylon rope is always kept in a linear state, thereby improving the measurement accuracy.

The invention relates to method and kits for highly specific isolation of surface marker displaying agents by targeting at least two surface markers. The invention further relates to methods and kits for analyzing surface marker displaying agents and their contents.

Systems and methods for determining a head pose of a user are described. Images captured by a head-mounted camera worn by the user are received, the images comprising captured markers, wherein each captured marker is one of a plurality of markers on a surface that are in the FOV of the head-mounted camera. Stored markers having a predetermined orientation and corresponding to each captured marker are identified from a database, the stored markers comprising coordinates for its corresponding surface marker. An orientation of the captured marker relative to its corresponding stored marker is calculated and the stored marker is obtained. Based on the stored marker and the orientation, an absolute pose of each marker is determined. Based on the absolute pose of each marker, the head pose of the user relative to the surface is calculated.

This invention discloses a method for the exploration and analysis of karst collapse geological hazards, specifically relating to the field of geological hazard exploration and analysis. The method includes: after collecting current and historical images of a target area on a cloud-based collaborative platform, performing image pairing based on shooting location, shooting orientation, and coverage boundaries; dividing the overlapping areas of each image pair into land tables; and outputting image pairs and land table sequences. For land tables with the same sequence number in each image pair, extracting the boundaries of open water, wet soil, bare soil, vegetation, and shadow, and writing a single land surface marker based on the first digit of the enclosed area. This invention addresses the problem in existing technologies where inconsistent surface conditions between different exploration images are easily misjudged as collapse evolution changes by performing image pairing, land table division, single land surface marker generation, non-comparable grid re-sampling and backfilling, and comparable grid segment change determination.

The present invention relates to a method for generating an enriched population of early cortical neural stem cells (NSCs) or a subpopulation thereof, the method comprising: isolating cells that are positive for the cell surface marker protogenin (PRTG) from an initial population of neural progenitor cells, wherein said isolating is conducted at a time point between about day 4 and about day 12, preferably on day 5, after initiation of neural induction, thereby obtaining an enriched population of early cortical neural stem cells (NSCs); and optionally re- culturing said enriched population of early cortical neural stem cells (NSCs) in a neural induction medium or other culture medium, wherein said re-culturing preferably produces progeny of said early cortical neural stem cells (NSCs).