156 results about "Placenta" patented technology

The preparation method comprises the following steps: (1) fish skin pretreatment: (2) gas foaming treatment: weighing sodium borohydride with a certain molar concentration, dissolving the sodium borohydride in a phosphate buffer solution with a certain concentration, stirring until bubbles in the solution are obviously generated and most of solids are dissolved, and then, carrying out gas foaming treatment; putting the cleaned fish skin tissues in the step (2), and performing foaming treatment; (3) cleaning treatment; and (4) protection and storage. The gas foaming technology is innovatively adopted for decellularization treatment, the interface stripping effect between cells and a matrix is remarkably enhanced on the premise that the collagen three-dimensional structure and the scaffold morphology integrity are not damaged, and the method is suitable for the decellularization pretreatment process of soft tissue (such as fish skin and placenta) with a compact structure or cell-intensive type. The development of a biomedical material, especially a tissue engineering scaffold technology, is promoted, and the preparation method has a wide application prospect.

The invention provides a construction method and application of a gene Idh1 conditional knockout mouse model and a preeclampsia heart injury animal model, and relates to the technical field of biology. According to the preeclampsia heart injury animal model, flox mice with conditional gene Idh1 knockout and mice with placental trophoblast specific expression Cre recombinase comate, PE-related phenotypes and typical symptoms of heart injury spontaneously appear in the double-positive pregnant mouse model, and the double-positive pregnant mouse model can be used for research of disease causes, diagnosis methods and drug research and development.

The invention belongs to the technical field of aquatic product breeding, and particularly relates to a KASP molecular marker related to high temperature resistance character of patinopecten yessoensis and application of the KASP molecular marker in genetic breeding. On the basis of genome re-sequencing of 200 patinopecten yessoensis, a PySNP587919 locus obviously associated with the patinopecten yessoensis high-temperature-resistant character is screened by combining a whole genome SNP genotype and whole genome association analysis of the patinopecten yessoensis high-temperature-resistant character, and after the reliability of the locus is verified through Sanger sequencing, the locus is developed into a KASP marker, and the KASP marker can be used for identifying the high-temperature-resistant character of the patinopecten yessoensis and identifying the high-temperature-resistant character of the patinopecten yessoensis. The screening method can be used for screening the high-temperature-resistant comb shells. Furthermore, the invention also establishes a method for screening the high-temperature-resistant patinopecten yessoensis, and the method is efficient and accurate. Therefore, the technical scheme provided by the invention is suitable for molecular marker-assisted breeding work, rapid and effective screening of parents can be realized by typing the PySNP587919 site, and the breeding efficiency is improved.

The invention relates to the technical field of stem cell extraction, in particular to a stem cell extraction process, solves the problems that when stem cells in a placenta are extracted in the prior art, the temperature in the placenta treatment process is inconvenient to keep stable, and the placenta is inconvenient to fully crush and clean, and is used for extracting the placenta stem cells. The stem cell extraction process is realized through stem cell extraction equipment, the stem cell extraction equipment comprises a circulating cooling mechanism, a coarse crushing mechanism, a fine crushing mechanism and a digestion separation mechanism, the coarse crushing mechanism is mounted on the inner side of the upper end of the circulating cooling mechanism, the fine crushing mechanism is mounted at the bottom end of the coarse crushing mechanism, and the digestion separation mechanism is mounted at the bottom end of the fine crushing mechanism. A digestion and separation mechanism is mounted on one side of the fine crushing mechanism, and the circulating cooling mechanism comprises a supporting base. Through continuous cleaning and gas injection in the stem cell extraction process, the temperature stability of the stem cells can be effectively kept, and the placenta cleaning and crushing effects can be conveniently improved.

This invention provides a traditional Chinese medicine composition for the prevention and treatment of knee arthritis. The active ingredients, by weight, include: 15-25 parts of Salvia miltiorrhiza, 8-15 parts of Ligusticum chuanxiong, 12-20 parts of Angelica sinensis, 7-15 parts of Lycopus lucidus, 15-30 parts of Poria cocos, 8-15 parts of Placenta hominis, 18-30 parts of Clematis chinensis, 15-25 parts of Spatholobus suberectus, 25-40 parts of Paeonia lactiflora, 12-20 parts of Rehmannia glutinosa, and 8-15 parts of Glycyrrhiza uralensis. Using Salvia miltiorrhiza, Ligusticum chuanxiong, Angelica sinensis, Lycopus lucidus, Poria cocos, Placenta hominis, Clematis chinensis, Spatholobus suberectus, Paeonia lactiflora, Rehmannia glutinosa, and Glycyrrhiza uralensis as raw materials, this invention follows the principle of "principal, assistant, adjuvant, and guide" in traditional Chinese medicine. The combined use of these herbs can tonify the liver and kidneys, promote blood circulation and remove blood stasis, and has a good therapeutic effect on knee arthritis caused by liver and kidney deficiency and qi stagnation and blood stasis.

The invention discloses a preparation method of a boiling antigen for vibrio parahaemolyticus serum agglutination. The preparation method comprises the following steps: selecting common serotype vibrio parahaemolyticus in monitoring for resuscitation; scraping a ring of placenta by using 10 mu L of an inoculating loop, dissolving the placenta in 1 mL of normal saline to prepare a bacterial suspension, and preparing 3 tubes of bacterial suspensions from each strain; boiling the bacterial suspension in boiling water for 1 hour, 45 minutes and 30 minutes respectively; and after boiling, centrifuging at 10000 rpm for 10 minutes, discarding the supernate, and reserving the precipitate as a boiling antigen for serum agglutination. The result shows that the agglutination accuracy after boiling for 1 hour is 100%, and the agglutination degree is 2 + or above; and no significant difference exists between 45 minutes of boiling and 1 hour of boiling, and no significant difference exists between 30 minutes of boiling. Compared with a high-pressure method, the method has the advantages that the operation time is shortened by 2-3 hours, special equipment certificates are not needed, and the method is suitable for daily detection and food poisoning traceability.

The invention provides an application of a pair of immune costimulatory factors TNFSF9 / TNFRSF9 as markers in preparation of a product for early prediction of preeclampsia, and the immune costimulatory factors comprise a tumor necrosis factor superfamily member 9 (TNFSF9) and a tumor necrosis factor receptor superfamily member 9 (TNFRSF9). The product is used for carrying out early warning on the preeclampsia occurrence risk 20 weeks before pregnancy. The product is used for detecting the immune costimulatory factor through at least one of serum, plasma, whole blood and placental tissue. The invention aims to improve the early prediction efficiency of preeclampsia by detecting the abnormal expression level of the pair of markers.

This disclosure generally relates to methods of preparing a graft for wound healing. In some embodiments, the methods comprise obtaining a donor placenta and isolating tissue comprising amnion and / or chorion from the donor placenta. In other embodiments, the methods comprise washing the isolated tissue with a cleansing solution. Other embodiments, still, are directed toward applying a current to the isolated tissue and / or cleansing solution to produce the graft for wound healing.

The utility model relates to the technical field of biology, and particularly discloses a camel placentin effective component extraction device which comprises a homogenate mechanism which comprises a liquid storage tank assembly, a stirring assembly installed on the liquid storage tank assembly, an interception assembly clamped and embedded in the liquid storage tank assembly in a sliding mode and an electric push rod used for driving the interception assembly to ascend and descend; the filtering mechanism comprises a filtering box assembly; the output end of the electric push rod is controlled to push the connecting plate to move upwards, the connecting plate drives the intercepting plate on the guide rod to move upwards, at the moment, the intercepting plate does not block the discharge port any more, camel placenta tissue homogenate in the liquid storage tank body flows into the filter tank body through the discharge port, and after being filtered by the microporous filter membrane, the camel placenta tissue homogenate flows into the filter tank body. Solid particles, impurities and cell debris in homogenate are intercepted above the microporous filter membrane, and liquid flows out of the bottom of the filter box body after passing through the microporous filter membrane for next centrifugal treatment, so that the separation purity is improved.

The present invention discloses a composition for treating hypothyroidism. The formula includes: placenta, antler, Pseudostellaria baicalensis, Salvia miltiorrhiza, and Herba Lycopodii. The prescription of the present invention is demonstrated to have therapeutic effects on hypothyroidism from both traditional Chinese medicine and modern medicine perspectives: from the perspective of traditional Chinese medicine, the efficacy of the present invention in replenishing qi and blood, regulating yin and yang, tonifying kidney essence, filling the marrow sea, and unblocking meridians is unique in treating goiter. From the perspective of modern medicine: Panlushending Jiawei Decoction has the effects of promoting cell reproduction, differentiation, and repairing damaged cells, and is also anti-apoptotic. It also has the effects of promoting the regeneration of vascular endothelial cells, forming new blood vessels, increasing blood flow rate, increasing blood supply, and increasing the secretion and synthesis of thyroid hormones. It can also increase the level of free hormone reabsorption from the intestine and lower the concentration of antibodies.

This invention is directed to a multi-layered tissue graft comprising a collagen layer and at least one separated and washed placental tissue component and / or umbilical cord component, wherein the collagen is human collagen substantially free of non-human antigens.

The invention discloses a method for extracting bioactive substances from sheep placenta, and relates to the technical field of animal placenta extraction. The method uses immobilized lipase to enzymolyze the crushed fresh sheep placenta, then adds protease to continue enzymolysis, and after the enzyme is inactivated, the purified bioactive substances have a high yield; and the immobilized lipase prepared by the chitosan derivative modified by the thiazole group has good application stability; in addition, the present invention also uses S-benzyl-L-cysteine ​​methyl ester hydrochloride to undergo a substitution reaction with a polyamide nanofiltration membrane, and the obtained nanofiltration membrane is used in the purification process of the bioactive substance, which has a good decolorization effect.

The invention discloses a construction method and application of an enterovirus 71 type susceptible transgenic mouse model, and belongs to the technical field of biology. The method comprises the following steps: up-regulating or enhancing or improving the content or / and activity of placenta specific protein 8 in a receptor mouse to obtain a mouse overexpressing the placenta specific protein 8, hybridizing the mouse overexpressing the placenta specific protein 8 with a wild mouse to obtain an F1-generation heterozygote mouse, a homozygote mouse obtained after the F1-generation heterozygote mice are hybridized with each other is an enterovirus 71 type susceptible transgenic mouse model. The application has the advantages that not only is the susceptibility of mice to EV71 increased, but also a proper animal model is provided for exploring the important function of Plac8 in the EV71 infection process.

The invention relates to the field of biological medicines, relates to a treatment technology for early abortion, and in particular relates to an application of Clostridium prausnitzii (Faecalibacterium prausnitzii) in preparation of a medicine for treating early abortion. An experiment of a Poly (I: C) induced early-stage abortion mouse model proves that the clostridium praeparatum can obviously reduce the embryo loss rate and improve placenta development through oral intragastric administration. The action mechanism is that maternal immune balance is regulated by increasing the proportion of regulatory T cells (Treg) and inhibiting excessive activation of an IL-17 signal channel. As human intestinal symbiotic bacteria, the clostridium praeparatum is high in safety and can be used for intervention of early abortion, especially recurrent abortion, and a new strategy is provided for clinic.

This invention belongs to the field of biotechnology and discloses a nucleic acid aptamer that specifically recognizes placental-specific protein 1 and its uses. The nucleotide sequence of the nucleic acid aptamer includes any one of the sequences shown in SEQ ID No. 1-57. This invention employs X-aptamer screening technology, combined with high-throughput sequencing technology and bioinformatics analysis, to reduce the number of screening rounds and obtain candidate nucleic acid aptamers. Further analysis of their affinity and specificity yields a nucleic acid aptamer that specifically recognizes placental-specific protein 1. The nucleic acid aptamer of this invention has the characteristics of high affinity and specificity, good stability, convenient synthesis, and easy labeling of functional groups. It can be used for the detection or binding of placental-specific protein 1, the diagnosis of placental-specific protein 1-related diseases, and the preparation of products for prognosis.

The present invention relates to the medical and food use of bile acid derivatives. Specifically, the present invention relates to the use of 3-oxoLCA in reducing oxidative stress and inflammation in pregnant mothers and their offspring. Specifically, the present invention relates to the use of 3-oxoLCA to reverse the effects of oxidative stress on pregnant mothers, in particular, to maintain fetal equal weight, significantly reduce placental and liver oxidative stress in pregnant mothers and the resulting inflammation or dysfunction of the placenta and liver, and to improve the intestinal flora imbalance of pregnant mothers. Further studies have found that 3-oxoLCA has long-term effects, in particular, can significantly reduce the effects of oxidative stress during pregnancy on oxidative stress and inflammation in the liver of offspring.

The present application relates to the field of traditional Chinese medicinal materials, in particular to a composition for resisting PRRSv and improving the reproductive performance of sows and application thereof. The present application formulates a composition composed of 10 plant extracts (Angelica sinensis extract, Atractylodes macrocephala Koidz extract, Glycyrrhiza uralensis Fisch extract, Eucommia ulmoides Oliver extract, Eucommia ulmoides leaf extract, Populus davidiana Dode extract, Perilla frutescens leaf extract, Gynostemma pentaphyllum Makino extract, Leonurus japonicus extract and Astragalus membranaceus Bge extract) for sows in late pregnancy, and collects sow blood and placenta samples at the time of delivery, studies the influence of the composition on sow reproduction, and explores the mechanism of action. The experimental results show that the composition can improve the reproductive performance of sows by increasing the total number of piglets and other indicators; meanwhile, the composition can effectively inhibit the replication of PRRSv.

The invention develops a construction method of a novel preeclampsia prediction model based on insulin growth binding factor binding protein 1 (IGFBP1) and placental growth factor (PlGF) and related application of the novel preeclampsia prediction model. Specifically, according to the method, IGFBP1 and / or PLGF of a pregnant woman, clinical information and MAP data serve as input, whether the pregnant woman suffers from preeclampsia or not serves as output, model construction is conducted through a machine learning method, and a preeclampsia prediction model is obtained. As effective detection can be carried out only by adopting one or two protein predictive markers without depending on the uterine artery pulsation index, the cost is low, and on the whole, clinical popularization is more convenient, the medical expenditure is reduced, the mom and infant outcome is effectively improved, and the method has important significance on reducing the mortality rate of pregnant and lying-in women and infants.

This invention discloses a culture medium, preparation method, and culture method for the industrial-scale preparation of mesenchymal stem cells, belonging to the field of cell therapy technology. The culture medium comprises a basal medium, cell growth factors, cell proliferation promoters, a vitamin mixture, a nucleoside mixture, and an amino acid mixture; wherein the cell growth factors are selected from one or more of TGF-β, bFGF, VEGF, PDGF, EGF, and IGF. Through the synergistic effect of the composite nutrient system, this invention significantly improves the proliferation rate and cell viability of mesenchymal stem cells, maintains stem cell stemness, trilineage differentiation ability, and secretion levels of key cytokines (VEGF, HGF, TGF-β, IL-10, etc.); it can be stably and continuously passaged up to the 15th generation without abnormal changes in genetic material. This culture medium contains no animal-derived components, has high batch-to-batch stability, and is cost-controllable, making it suitable for GMP-level large-scale industrial preparation of mesenchymal stem cells from various sources such as umbilical cord, bone marrow, adipose tissue, and placenta.

This invention relates to the field of bioengineering technology, and in particular to a method for simultaneously collecting the inner cell mass and trophectoderm of mammalian blastocysts. The method includes the following steps: (1) after removing the zona pellucida of the blastocyst, the blastocyst is cut into an inner cell mass and a trophectoderm portion, and the cut trophectoderm portion is transferred to a culture medium for collection; (2) the inner cell mass portion obtained in step (1) is sequentially placed in an antibody treatment solution and a complement treatment solution for incubation; (3) the inner cell mass portion treated in step (2) is pipetted to obtain a purified inner cell mass. This method achieves simultaneous and efficient separation of two key cell lines from the blastocyst, fundamentally changing the traditional method's "resource waste" model of only obtaining the inner cell mass while discarding the trophectoderm, and providing more comprehensive experimental materials for embryonic development, placental biology, and stem cell research.

The invention relates to methods for identifying neonates at an increased risk of neonatal sepsis by screening for the presence of Group B Streptococcus (GBS) in a sample of placenta. The invention also relates to nucleic acid-based detection methods for detecting Streptococcus agalactiae (Group B Streptococcus, GBS) and to compositions and kits for use in said methods.

The invention relates to a method for constructing a preeclampsia prediction model by using clinical laboratory data and a related application thereof. Specifically, the preeclampsia risk prediction model is constructed based on a plurality of clinical indexes of pregnant women, a plurality of clinical laboratory data and insulin growth binding factor binding protein 1 and / or placental growth factors. The clinical laboratory data comprises leukocyte count and / or urine protein, and further comprises any one or combination of platelet count, monocyte proportion, glutamic-straw / glutamic-pyruvic transaminase ratio, glutamyltranspeptidase, uric acid and high-density lipoprotein. The constructed preeclampsia risk prediction model can provide a reliable implementation means for early prevention and early treatment of preeclampsia. Meanwhile, the sensitivity of the prediction model is 88.0% when the specificity of early eclampsia is 85.04% and the sensitivity of the prediction model is 62.5% when the specificity of late eclampsia is 85.04% in a complete independent verification set, and the detection performance of the prediction model is higher than that of the current detection performance.

The present application relates to the technical field of biological medicine, and more particularly to the application of isodon japonicus in the preparation of a drug for preventing and treating spontaneous abortion. The present application analyzes the in-vivo migration components of Jianwei Shoutai Pills, excavates the pharmacodynamic material basis for preventing and treating spontaneous abortion, and uses a CBA / J x DBA / 2 peri-implantation period abortion mouse model to conduct pharmacodynamic experiments. The results show that isodon japonicus can significantly reduce the abortion rate of the CBA / J x DBA / 2 peri-implantation period abortion model mice, and can effectively increase the embryo and placenta wet weight of the CBA / J x DBA / 2 peri-implantation period abortion model mice. That is, isodon japonicus can effectively inhibit spontaneous abortion, has a significant curative effect, has considerable treatment potential, provides a potential new drug selection for clinical drugs for preventing and treating spontaneous abortion, and has a broad application prospect.

The invention discloses a method for extracting and separating cord blood and placenta stem cells of sika deer, and belongs to the technical field of biomedicine. The problems that an existing stem cell extraction and separation method is low in efficiency, poor in cell activity, low in purity and large in pollution risk are solved. The method comprises the following steps: collecting cord blood and placenta after delivery of sika deer; diluting the cord blood, performing density gradient centrifugation, collecting the albuginea layer enriched with the stem cells, adding an erythrocyte lysate to remove residual erythrocytes, and centrifuging and collecting the stem cells; the method comprises the following steps: cutting placenta tissues into pieces, putting the placenta tissues into trypsin-EDTA digestive juice containing collagenase, carrying out ultrasonic-assisted digestion, and centrifuging to obtain stem cells enriched at the bottom of a centrifugal tube; and finally, resuspending the collected stem cells in a culture medium containing fetal calf serum and growth factors, adjusting the cell concentration, and inoculating into a cell culture bottle for culture. According to the method, the extraction efficiency and quality of the sika deer cord blood and placenta stem cells can be remarkably improved, and the activity and safety of the cells are ensured.

The present invention provides a compound or derivatives thereof capable of lowering circulating glucose in a subject. The compound is a recombinant placenta-specific protein 9 (Plac9) or derivatives thereof that can enhance glucose uptake by liver cells or hepatocytes in an insulin-independent manner, thereby lowering the circulating glucose. The present invention also provides a composition including an effective amount of the compound or derivatives thereof and a method for treating diseases or conditions associated with or arising from high circulating glucose such as diabetes mellitus.

The application provides a placenta perfusion and fetal development prediction method and system based on ultrasound images, and relates to the technical field of medical image analysis and diagnosis, which comprises the following steps: obtaining a placenta ultrasound image and pre-processing and quality control; segmenting the placenta region through a deep neural network, extracting multi-dimensional features of gray scale, texture, shape and blood flow; inputting a machine learning model to predict the perfusion and fetal development state; visualizing the results and key factors, combining clinical verification and generating intervention suggestions. The system comprises image processing, segmentation, prediction, visualization and verification interpretation modules. The application realizes objective quantitative evaluation, solves AI problems, forms full-chain clinical support, improves early warning accuracy, helps precise intervention and reduces perinatal risks.

A cell population including macrophages that are CD11b positive, F4 / 80 positive, CD180 positive, and CD9 positive and that are derived from a tissue selected from the group consisting of placenta, umbilical cord, and amnion with a percentage of 75% or more, the cell population having cell viability of 80% or more.

The invention discloses an mRNA (messenger ribonucleic acid) translation enhancement tool (MiniCURE) and application thereof, and belongs to the technical field of gene editing. The Mini CURE provided by the invention utilizes a natural m6A-YTHDF1 pathway for regulating mRNA stabilization and translation, the off-target effect generally related to a DNA editing tool is avoided, and data confirms that the Mini CURE has an effective effect of increasing protein expression. Transcriptomic and proteomic analysis proves that the minimum off-target activity is achieved, and compared with a control group, only six proteins in the placenta treated by Mini CURE are up-regulated. The high specificity is crucial to clinical translation because maternal or fetal complications may be aggravated by whole body disturbance.

The invention discloses hydrogel based on umbilical cord tissue and chorion tissue, which is prepared by a method comprising the following steps: (1) taking a placenta, shearing the umbilical cord and separating the chorion; (2) respectively carrying out tissue crushing on the umbilical cord and the chorion, and centrifugally collecting precipitates; mixing, washing, centrifuging and collecting precipitates; (3) freezing with liquid nitrogen, and then melting; repeatedly freezing and thawing; adding a buffer solution containing triton-X-100, and carrying out soaking treatment; rinsing, centrifuging and collecting precipitates; (4) adding urea, oscillating, centrifuging and collecting precipitate; freezing and drying to obtain freeze-dried powder; (5) dissolving the freeze-dried powder in hydrochloric acid, adding pepsin, and reacting; and adjusting the pH value to 7-7.5 to obtain the hydrogel. The hydrogel is applied to culture of cells, tissues or organoids, and is applied to preparation of a culture medium with the effect of promoting angiogenesis. The method is of great significance to development in the fields of regenerative medicine, disease modeling, medicine development and the like.