75 results about "Cell extraction" patented technology

The invention discloses a genome sequencing sample processing method based on a microdissection technology. The method comprises the following steps: (1) preparing a paraffin section or a frozen section; (2) carrying out HE staining on the section obtained in the step (1); (3) carrying out laser microdissection on nucleic acid; (4) fragmenting the DNA sample, and treating a product obtained in the step (3) by utilizing an ultrasonication method to obtain a DNA fragment; a non-contact ultrasonic crusher is adopted, the power is 80%, ultrasonic treatment is carried out for 10 s, stopping is carried out for 20 s, and 15 cycles are carried out. And (5) carrying out on-machine pretreatment on the genome sequencing sample, wherein the treatment comprises tail end repair and joint connection treatment. According to the method disclosed by the invention, accurate cutting of the sample can be realized, a DNA sample is extracted from a very small amount of cells and a sample for sequencing is constructed, the sample can achieve the same or higher sequencing data quality as that of a traditional method, the usage amount of the cells is greatly reduced, and the repeatability of an experiment is improved.

The invention relates to an extraction and culture method of urine-derived stem cells, and belongs to the field of cell biology and regenerative medicine. The method comprises the following steps: coating a culture plate with a gelatin solution; collecting clean midstream urine of healthy people and adding antibiotics; centrifugally separating the cell precipitate; washing with a phosphate buffer solution; re-suspending the precipitate by using a urine stem cell complete culture medium for primary culture; and after the cells grow to a certain fusion degree, carrying out passage amplification. The urine stem cell complete medium comprises a basic medium, fetal calf serum, epidermal growth factors, platelet-derived growth factors and basic fibroblast growth factors. By adopting the method disclosed by the invention, the urine-derived stem cells can be efficiently and stably separated from urine, and the obtained cells have high multiplication capacity, good stemness maintaining capacity, multidirectional differentiation potential and immunoregulation function, and can provide an ideal seed cell source for treating diseases such as diabetic erectile dysfunction.

The invention discloses a ship declaration cooperative processing system and method based on a multi-user role, and relates to the technical field of ship declaration, and the method comprises the steps: instantiating a digital twin software object for a new ship declaration request; extracting core semantic assertions, metadata and semantic potential energy parameters, packaging the core semantic assertions, the metadata and the semantic potential energy parameters into a semantic cell data unit, traversing all semantic cells in the digital twin, extracting the core assertions of the semantic cells, carrying out semantic matching on the core assertions and nodes in the domain knowledge graph, finding out a corresponding knowledge graph standard concept node, determining an acting force rule, and carrying out semantic analysis on the acting force rule; all semantic cells are placed in a semantic field, and each cell is endowed with a charge attribute obtained through mapping of the knowledge graph according to the core assertion of the cell; and the system runs a physical simulation cycle until the system reaches a steady state or a quasi-steady state, the total potential energy of the whole cell system is calculated, the total potential energy of the system is compared with a preset threshold interval through a normalization function and a calculated semantic stability score, and a decision branch is automatically triggered.

The present invention is related to the field of tissue regeneration. It concerns more particularly new processes, tubes and devices for thrombin, platelet concentrate and wound healant preparations, alone or in combination with cell extracts, cell compositions and uses thereof.

The application relates to the technical field of biological sample pretreatment, and discloses a high-efficiency separation method of a tissue cell extraction device. The method comprises the following steps: performing parameter calibration and reference loss modeling of a driving motor, and eliminating background interference of an actuator; applying a disturbance signal to a dissociation chamber, locking the characteristic resonance frequency of a tissue sample through frequency domain energy analysis, and performing pulsating crushing to accelerate the dissociation of a matrix structure; extracting the net torque of the motor in real time, deducing the fluid dynamic viscosity, adaptively adjusting the motor speed according to the viscosity drop trend, maintaining the fluid shear force in a preset target interval, and avoiding mechanical damage to cells; calculating the mechanical energy dissipation power in real time, and performing feedforward temperature control compensation to eliminate temperature overshoot caused by thermal conduction lag. The application realizes closed-loop control of the physical phase evolution of a heterogeneous tissue dissociation process, solves the limitation of a fixed parameter in a traditional method, shortens the dissociation time, and significantly improves the single-cell yield and survival rate.

This invention relates to the field of battery processing technology, and in particular to a multi-station blade battery cutting and core extraction device, comprising an integral processing frame. A cutting unit, a cross-cutting unit, and a core extraction unit are respectively mounted and connected on the upper surface of the integral processing frame. A material transfer bracket is fixedly mounted on the upper surface of the integral processing frame. Parallel linkage between the units is achieved through a linear slide module. During longitudinal cutting, the material transfer mechanism is synchronously ready and immediately performs the material transfer action after cutting, without any downtime. The material transfer, clamping, cutting, and flipping actions of the cross-cutting unit are continuously connected. The core extraction unit and the cross-cutting unit seamlessly coordinate their posture adjustments. The entire process requires no manual intervention. From battery loading, positioning, and cutting to cell extraction and material diversion, a closed-loop operation is formed. Simultaneously, the timing of each executing component is precisely controllable, effectively shortening the cycle time of a single process, meeting the capacity requirements of large-scale mass production and recycling / dismantling, and significantly reducing labor costs and operational risks.

This invention discloses a multi-enzyme preparation for industrial cell dissociation, its usage method, and applications, belonging to the field of seed cell extraction technology for industrial meat culture. The invention employs a multi-enzyme preparation composed of component 1 and component 2; component 1 includes neutral protease, hyaluronidase, and elastase; component 2 includes matrix metalloproteinase, papain, and plasmin. The invention also discloses the preparation method and applications of the multi-enzyme preparation. The rational enzyme ratio and optimized treatment conditions of this invention help protect cell membrane integrity, maintain high cell activity, and significantly improve dissociation efficiency and cell recovery. The compound enzyme preparation is highly adaptable, applicable to various tissue types and dissociation requirements, and facilitates standardized and large-scale production. Combined with automated dissociation equipment, the compound enzyme preparation enables efficient and stable industrial operation, providing important technical support and broad application prospects for the cultured meat industry, tissue engineering, and cell therapy.

The utility model discloses a primary cell extraction device, which relates to the technical field of cell extraction, and comprises a cell centrifugal machine and a collecting device, the bottom of the cell centrifugal machine is fixedly connected with the top of the collecting device, in the utility model, the device can automatically cut and crush a cell sample through the reverse rotation of a left group of cutters and a right group of cutters; manual intervention is greatly reduced, the cell extraction efficiency is improved, meanwhile, the crushing consistency of a packaged sample can be achieved, the purity and yield of subsequent cell extraction are improved, a cell centrifugal machine is started according to a preset separation program for primary cells, the centrifugal process is automatically completed, layering of the cells and impurities is achieved, and after centrifugation is finished, the cell extraction efficiency is improved. Separated cells and supernate can be accurately collected into corresponding containers through the collection device, tissue smashing, cell separation and collection are integrated in one device, the sample transfer frequency is reduced, the pollution risk is reduced, and the working efficiency is improved.

This invention provides a method for processing live single-cell extraction based on deformation trends, comprising the following steps: Step S1: Controlling a microfluidic chip to immerse itself in a culture medium containing adherent cells while maintaining a preset gap, controlling the injection flow rate of the injection channel and the aspiration flow rate of the aspiration channel to form a microjet acting on the cells; Step S2: Controlling an image processing module to receive and process cell morphology images on the culture substrate acquired by a microscopic imaging module, and calculating deformation parameters based on the comparison between the cell area in the current frame image and the initial cell area; Step S3: Comparing the deformation parameters with critical deformation parameters obtained through experimental calibration, and when the deformation parameters are greater than the critical deformation parameters, controlling a fluid drive module to reduce the injection flow rate and / or the aspiration flow rate; or, calculating a comprehensive damage risk index based on the deformation parameters, and when it is greater than 1, reducing the injection flow rate and / or the aspiration flow rate.

The application discloses an enhanced red blood cell lysis solution, a red blood cell DNA extraction kit and a method. The application provides an enhanced red blood cell lysis solution, which can improve the yield of red blood cell DNA. The application also provides a red blood cell DNA extraction kit and discloses a method for separating or extracting DNA from red blood cells. In addition, the application also discloses applications of the kit and the method for separating or extracting DNA from red blood cells, and the applications include applications in detecting cancer or predicting the risk of suffering from cancer. The enhanced red blood cell lysis solution and the kit provided by the application have important scientific value and application prospect.

This invention provides a method for determining the stacked cell hierarchy based on contour completion and gradient quantization. The method involves extracting the visible contour point set of each cell from a cell microscopic image; performing ellipse fitting on the visible contour point set of each cell to obtain the fitted ellipse parameters for each cell, including center coordinates, major axis length, minor axis length, and rotation angle; calculating a global gradient magnitude map on the cell microscopic image; filtering candidate cell pairs based on the fitted ellipse parameters, including overlapping cell pairs and adjacent cell pairs; for each cell pair in the candidate cell pairs, extracting the contour points of each cell within the overlapping region, obtaining the gradient values ​​corresponding to the contour points from the global gradient magnitude map, and calculating the average gradient value for each; determining the hierarchy relationship between the two cells by comparing the average gradient values; constructing an attribute graph using each cell as a node and the hierarchy relationship as an edge; performing topological sorting on the attribute graph; and outputting a global stacked hierarchy sequence.

The invention provides a degradable polylactic acid-based human cell sampling brush wire material and a preparation method thereof, and relates to the technical field of preparation of medical supplies. The degradable polylactic acid-based human cell sampling brush wire material is prepared from the following raw materials: a composite modified material, polyethylene glycol, a plasticizer and an antibacterial agent, the composite modified material is obtained by mixing a product A prepared from polylactic acid and maleic anhydride and a product B prepared from polylactic acid and PBAT and then carrying out ultraviolet treatment, the plasticizer is one of glyceryl triacetate and acetyl tri-n-butyl citrate, and the antibacterial agent is chlorhexidine. The mechanical property of the prepared cell extraction brush wire material is improved while the existing characteristics of polylactic acid are not influenced, the elastic recovery rate is particularly improved, the cell extraction brush wire material has better elasticity and structural stability, and the structural stability and operability are kept when the cell extraction brush wire material is used for sampling positions such as cervix uteri; and the application value in cell extraction operation tools is comprehensively improved.

The utility model discloses a test tube placing rack for extracting and storing test cells, and relates to the technical field of cell extraction. The storage rack comprises a box body, a first storage rack body and a second storage rack body are placed on the upper side of the box body, the second storage rack body is located on one side of the first storage rack body, and L-shaped plates are fixedly matched with the two sides of the second storage rack body; the four square-shaped plates are in elastic and sliding fit with the upper side of the box body, and threaded rods are in running fit with the interiors of the square-shaped plates. Through the arrangement of the clamping plate, one end of the threaded rod drives one end of the clamping plate to slide in the square-shaped plate under the action of a worker, and one side of the L-shaped plate is fixed through the other end of the clamping plate, so that the problem that the second placing frame body shakes in the box body is reduced; and the fixing effect of the second placing frame body is improved.

The utility model provides a stem cell extraction constant-temperature culture device which comprises a main body mechanism and a culture mechanism arranged in the main body mechanism, the culture mechanism comprises a motor, a limiting box arranged at the output end of the motor, limiting gauzes arranged in the two sides of the limiting box, and a base arranged in the limiting box, the supporting blocks are arranged at the four corners of the top of the base, and the fixing pieces are arranged at the tops of the supporting blocks; when the stem cell culture box is used, after stem cells enter the culture box, the cover plate is rotated to enable the cover plate and the threaded ring at the top of the culture box to be screwed and fixed, the culture box is placed in the fixing piece and the limiting groove in the base, the base is placed in the limiting box for centralized culture, and meanwhile the motor drives the limiting box to rotate; meanwhile, when the stem cells are taken out, the culture box is independently taken out for taking the stem cells, and waste and pollution caused by excessive taking are prevented.

The invention provides a hybrid potato seedling hypocotyl single cell extraction method and application thereof. According to the characteristics of potato seedling hypocotyl single cells, the extraction efficiency of the protoplast is remarkably improved by adjusting the dosage of different cellulase in the enzymatic hydrolysate and combining a slight extrusion mode. The method is simple and rapid, does not need complex treatment and expensive reagents, and is convenient to popularize and apply. Besides, experiments prove that the protoplast extracted by the method can be effectively used for detection such as subsequent sequencing and the like, so that the cells extracted by the method are relatively high in activity and have a wide application value.

The invention provides a fluorescent silver nano-cluster molecule taking DNA as a template, and the DNA is DNA with a hairpin structure and comprises a C-rich sequence and a recognition sequence. The invention also provides a composition for detecting circular RNA in a sample, the composition comprises a first silver nanocluster molecular probe and a second DNA probe, and the second DNA probe comprises a nucleic acid molecule containing a G-rich sequence and a second recognition sequence combined with a BSJ region of the target circular RNA. The invention further provides a method and a kit for detecting the circular RNA and / or homologous single-stranded RNA of the circular RNA in a sample based on the fluorescent silver nano-cluster molecule. The detection method and the kit provided by the invention have good sensitivity and specificity, and can be used for detecting target circular RNA and homologous linear RNA in a cell extract.

The application discloses a multifunctional radar wave element extraction method and system based on a dot matrix model, and the method comprises the following steps: for a multifunctional radar (MFR) pulse sequence, a dot matrix (D) based on a pulse repetition interval (PRI) value, a pulse width (PW) value and a radio frequency (RF) value is respectively constructed according to the threshold values of the corresponding PRI value, PW value and RF value of the MFR pulse sequence; PRI ,D RF ,D PW ; the constructed dot matrix (D PRI ,D RF ,D PW is respectively subjected to image preprocessing, image hole filling and image closing operation; an L-shaped corner point on a main diagonal line of the preprocessed dot matrix is detected by using a Harris corner point detection operator, that is, a wave element starting point; and a union set of the detection results is obtained, that is, a wave element starting pulse set. The application does not depend on cluster parameter setting, can fully utilize the overall modulation law of the wave element, reduces the false alarm probability while improving the wave element detection probability, and still has good performance in the case that the pulse is lost or the parameter measurement error is large.

The invention discloses a tissue cell nucleus extraction method and a cell nucleus protective agent applied to flow sorting, and relates to the technical field of cell extraction.The tissue cell nucleus extraction method comprises the steps that operation instruments needed by an experiment are prepared and pre-cooled; cutting a fresh tissue sample into tissue blocks, and immediately transferring the tissue blocks into a precooled lysis buffer solution; manually grinding the lysis buffer solution containing the tissue block by using a homogenizer, and sampling and observing homogenate in the grinding process; filtering the homogenate product through a cell filter screen in sequence; performing low-speed centrifugation on the filtrate by adopting a centrifugal machine, and discarding supernatant to obtain primary cell nucleus precipitate; re-suspending the primary cell nucleus precipitate by using a cell nucleus washing buffer solution to obtain a purified cell nucleus precipitate; and precipitating and resuspending the purified cell nucleuses, counting by trypan blue dyeing, and calculating the percentage of the complete cell nucleuses. According to the method, the problem of more cell nucleus breakage in the tissue cell nucleus extraction process is solved through a mild homogenization strategy monitored under the microscope.

The invention relates to the technical field of single-cell sequencing, and discloses a high-quality single-cell Hi-C library building method, which comprises the following steps: by utilizing a cumulative effect, carrying out cross-linking immobilization, enzyme digestion, biotinylated nucleotide filling-in and connection on population cells, sorting single cells, immediately marking different bar code combinations on each cell, mixing extracts of each cell, and carrying out high-quality single-cell Hi-C library building. After mixing, the cell population is equivalent to a small amount of cell population, enriching biotinylated DNA by using streptavidin magnetic beads, and finally establishing a library. According to the method, the library quality can be remarkably improved while the single cell resolution is maintained.

The utility model relates to the technical field of cell extraction, and provides a centrifugal tube suitable for cell precipitation extraction, which comprises a first centrifugal tube, a second centrifugal tube and a fixing mechanism, the first centrifugal tube is detachably connected with the second centrifugal tube through the fixing mechanism, and scale marks are carved on the outer side walls of the first centrifugal tube and the second centrifugal tube; the fixing mechanism comprises a first connecting block and a second connecting block, the first connecting block is mounted on the first centrifugal tube, the second connecting block is mounted on the second centrifugal tube, and a plurality of limiting pieces are mounted at the top of the second connecting block. The centrifugal tube has the beneficial effects that the centrifugal tube is divided into two parts, namely the upper tube body and the lower tube body, and the two parts are detachably connected by adopting the fixing mechanism, so that the centrifugal tube can be easily assembled and disassembled, and the upper tube body can be slightly rotated to be separated from the lower tube body in cell precipitation extraction work, so that the extraction depth is reduced, and the extraction efficiency is improved. The bottom sediment can be more easily and completely taken out, and the cell sediment extraction work is facilitated.

Described herein are compositions and methods for in vitro detection of nucleic acids. Nucleic acid sensors are activated for cell-free expression of an encoded reporter protein based on the presence of a target nucleic acid. The system is designed to function in low-cost cell extract without the need for instrumentation or stringent temperature control. These features are advantageous for point-of-use molecular diagnostics applications in consumer health, pet / animal health, food safety, and other areas where cost and portability are key factors.

This invention discloses a bioactive composition with synergistic effects. The composition comprises a highly active stem cell extract prepared by a sequential pretreatment of hypoxia combined with heat shock, and a high-affinity monoclonal antibody P3F6 targeting human PTEN protein. The stem cell extract is rich in heat shock proteins and various growth factors; the monoclonal antibody P3F6 possesses specific light and heavy chain variable region sequences. Experiments have demonstrated that this composition exhibits significant synergistic effects in promoting skin cell proliferation, inhibiting apoptosis, enhancing skin barrier function, and promoting collagen synthesis, with effects far exceeding those of single components. This invention also provides the application of the composition in the preparation of skin repair, anti-aging cosmetics, and medical wound healing dressings.

The invention relates to the field of biological pharmacy, in particular to a composition for cell-free protein synthesis and application thereof. The composition for cell-free protein synthesis comprises multiple of the following components: ribonucleic acid, folic acid, oxalic acid, putrescine, spermidine, potassium salt, magnesium salt, ammonium salt, amino acid, PEG8000 and a cell extract. According to the preparation method of the antibody coupling medicine, the raw materials are simple and easy to obtain, the universality is high, and the problems that in a traditional method, a full-length antibody has four pairs of disulfide bonds which are conveniently used for fixed-point coupling, and a single-domain antibody has no exposed disulfide bonds and is not prone to fixed-point coupling can be solved; therefore, the preparation method of the antibody coupling medicine can easily obtain the single-domain antibody coupling medicine with fixed-point coupling, good uniformity and various DAR values.

The present disclosure relates to a non-natural flavin-dependent oxidase that does not comprise a disulfide bond and that is capable of oxidative cyclization of a prenylated aromatic compound into a cannabinoid. In some embodiments, the non-natural flavin-dependent oxidase comprises: (i) at least 70% sequence identity to SEQ ID NO: 3; and (ii) substitutions at amino acid positions Q275, C285, V323, E370, V372, N400, D404, and T438, wherein the amino acid positions correspond to SEQ ID NO:3. The present disclosure further relates to a polynucleotide, an expression construct, and an engineered cell for making the non-natural flavin-dependent oxidase. Also provided are a composition comprising the non-natural flavin-dependent oxidase; an isolated non-natural flavin-dependent oxidase and methods of making the same; a cell extract or cell culture medium comprising the non-natural flavin-dependent oxidase; and methods of making a cannabinoid.

This invention relates to the field of cell biology, and more particularly to a method for constructing an artificial cell model in vitro based on extracts from Xenopus laevis egg cells. The method includes: mixing cytoplasmic extracts from Xenopus laevis egg cells, demembranous sperm, and tubulin, dispersing the mixture in an oil phase to obtain a reconstructed system; reacting the reconstructed system at 20-24°C for at least 30 minutes, resulting in the formation of a phospholipid bilayer-based cytoplasmic membrane structure around the cell. This invention provides a method for constructing artificial cells by selecting cytoplasmic extracts from Xenopus laevis egg cells, adding specific substances, and reacting under specific conditions to allow the extracts to self-assemble into cell structures in vitro, simultaneously forming a cytoplasmic membrane. The method provided by this invention offers important clues to the origin and evolution of cells, and the resulting artificial cell structures are highly similar to normal cells, possessing significant application value.

The invention belongs to the technical field of adipose-derived stem cell culture, and discloses an adipose-derived stem cell exosome culture device and a culture method.The adipose-derived stem cell exosome culture device comprises a culture box, a water bath box and a power control box which are two independent sealing boxes are arranged in the culture box, and a first sliding frame is slidably installed at the position, close to the upper end, in the water bath box; a plurality of mounting plates which are uniformly distributed at intervals are fixedly mounted on the first sliding frame, a constant temperature device is arranged on the inner wall, located below the mounting plates, of the water bath box, a double-output-shaft motor is fixedly mounted at the position, away from the water bath box, in the power control box, and the double-output-shaft end of the double-output-shaft motor is in transmission connection with a multi-connecting-rod device; the other end of the multi-connecting-rod device is fixedly connected with the first sliding frame; the whole structure is simple, the temperature control precision can be improved in the adipose-derived stem cell exosome culture process, batch culture can be achieved, the problems that adipose tissue blocks make contact with digestive enzymes unevenly, and the cell extraction rate is low are solved, and the use effect is improved.

The invention discloses a lymphatic endothelial cell cyst fluid extraction method for lymphatic deformity, and belongs to the technical field of biological cell extraction. Comprising the following steps: S1, sample collection: collecting 5-10 mL of cystic fluid of a patient with macrocystic lymphangial malformation, and placing the collected cystic fluid in a sterile container; s2, primary centrifugation: centrifuging at a low speed of 1000rpm for 10 minutes, and retaining cell precipitates; s3, red blood cell lysis: adding a red blood cell lysis solution, placing at 4 DEG C for 3-5 minutes, centrifuging, and washing and purifying with PBS (Phosphate Buffer Solution); s4, cell culture: resuspending the cells in a culture medium containing an endothelial growth factor replenishing liquid, culturing at 37 DEG C in 5% CO2, and changing the liquid for 24 hours to remove non-adherent cells; and S5, cell identification: carrying out immunofluorescence staining identification 48-72 hours after cell adherence. The method is simple, convenient and efficient to operate, does not need complex digestion, can enrich high-purity and high-activity lymphatic endothelial cells, provides a stable cell model for related pathogenesis research and drug screening, and has important scientific research and clinical value.

The invention discloses autologous fat transplantation equipment for increasing the survival rate of fat cells, and relates to the technical field of fat cell transplantation. Comprising an adipocyte transplantation processing mechanism, the adipocyte transplantation processing mechanism comprises a cell transplantation box, and a control panel is installed on the surface of the cell transplantation box; the device can conveniently extract transplanted and extracted fat cells, is beneficial to efficiently treating the transplanted fat cells, improves the fat cell transplantation and extraction effect, reduces the probability of errors caused by manual extraction of the fat cells by medical staff, can ensure that the fat cells are transplanted and extracted in a closed and sterile environment, and improves the survival rate of the fat cells. The fat cell transplantation and extraction device is simple in structure and convenient to use, the survival rate of fat cell transplantation and extraction is increased, medical articles for fat cell extraction can be stored, medical staff can conveniently operate in the fat cell transplantation and extraction work, the fat cell transplantation efficiency of the medical staff is improved, and convenience is provided for the staff.

A method for determining at least one structural and / or physicochemical property, or a property thereof, of at least one protein or peptide comprised in a complex mixture of additional proteins and / or other biomolecules in at least one cell, the method comprises the steps of: 1. Delivering a protease into the cells and subjecting the complex mixture to limited proteolysis followed by cell extraction and / or cell lysis to produce a fragment sample; 2. Denaturing the fragment sample to obtain a denatured fragment sample; 3. Optionally, completely fragmenting the denatured fragment sample in a digestion step to obtain a completely fragmented sample; and 4, analytically analyzing the fragmented sample or the completely fragmented sample to determine the at least one protein or peptide or the characteristics thereof.