177 results about "Cell extraction" patented technology

The invention belongs to the technical field of inspection and extraction, and particularly relates to an automatic T lymphocyte suction device which comprises an automatic suction device body, the automatic suction device body comprises a bearing table, a rotating disc with a circumferential rotating function is arranged on the bearing table, and a plurality of centrifugal assemblies are evenly distributed on the rotating disc. A centrifugal tube feeding assembly facilitating feeding of centrifugal tubes is arranged in front of one side of the bearing table, a tube carrying and conveying assembly is arranged on the portion, located on one side of the centrifugal tube feeding assembly, of the bearing table, a tube feeding mechanism is arranged at the end of the conveying belt mechanism, and a reaction reagent adding assembly is arranged on one side of the tube carrying and conveying assembly. A sample feeding assembly is arranged on one side of the upper portion of the workbench, and a first suction assembly is arranged above the multi-direction adjusting assembly. According to the invention, multiple samples can be processed in batches, the working efficiency and the cell extraction purity are improved, the influence of manual operation on the result is reduced, the accuracy of the detection effect is ensured, and the use requirements are met.

The present disclosure relates to the seminal discovery of a generation and use of genetically engineered Vibrio sp. Provided is the use of the genetically engineered bacteria for the construction, maintenance, manipulation, and / or propagation of DNA constructs; protein expression; protein secretion; vectors and other metabolic tools; metabolic engineering; expression of cellular extracts for cell-free biology; shuttle vectors; cloning vectors; and for synthetic biology applications. The disclosure also relates to the use of the replication machinery of Vibrio sp. as a cloning or expression vector for replication of recombinant DNA constructs. The disclosure also relates to methods of use of the above.

The invention belongs to the technical field of cell extraction, and particularly relates to a method for extracting cells by enzymolysis of vitreous bodies, which comprises the following steps: centrifuging a vitreous body sample at room temperature, discarding supernate, and adding digestive enzyme into precipitate; after adding digestive enzyme, digesting in a water bath for 30 minutes; and after the enzymolysis is finished, centrifuging the enzymatic hydrolysate at room temperature, discarding the supernate, and collecting the precipitate, namely the enzymolysis vitreous cells. According to the technical scheme, a simple and efficient vitreous body enzymolysis method is provided, and a large number of cells can be provided for follow-up research.

The present invention relates to a cartilage tissue single cell extraction kit and an extraction method thereof, the kit comprises: (1) a first enzyme for enzymatic hydrolysis, the first enzyme for enzymatic hydrolysis comprising one or more of trypsin, chondroitinase ABC and fucosidase; (2) a second enzyme for enzymolysis, wherein the second enzyme for enzymolysis comprises collagenase II, neutral protease II, hyaluronidase and Dnase I; and (3) a basic solution, wherein the basic solution comprises a basic culture medium and / or a buffer solution. The invention also provides a method for extracting single cells from cartilage tissues by using the kit. The cartilage tissue single-cell suspension prepared by the method has the advantages of high cell yield, high activity and the like, and has a wide application prospect.

The invention provides a lysosome extraction method. The lysosome extraction method comprises the following steps: (1) extracting cells; (2) tissue extraction; (3) crude separation of lysosome; and (4) separating the lysosome by magnetic beads. Through EDTA-free digestion optimization, lysis solution and buffer solution formula optimization, a filter membrane interception-magnetic bead purification combined technology, a double-antibody synergistic capture strategy and a competitive mild elution system, the four core problems of low purity, large activity damage, tedious operation and strong equipment dependence in a traditional lysosome separation technology are systematically solved. Experimental data show that the lysosome yield and functional integrity of the method are obviously superior to those of a density gradient centrifugation method, and the method is suitable for trace samples and conventional equipment and has high conversion value. In the future, the technical barrier can be further optimized through automation equipment integration and multi-dimensional function verification, and the application of the method in basic research and clinical diagnosis is promoted.

The system for dismantling the spent lithium-ion battery pack includes: a transfer section including transfer rails and a gantry robot on the transfer rails; a discharge processing section including an index table on which a discharging task of discharging a battery pack is performed; a battery disassembly section including a worktable on which a disassembly task is performed to cut connections of the battery pack, disassembly robots, and first transfer trolleys; and a cell extraction section including an extraction robot, a second transfer trolley configured to carry out battery cells, of which the connections have been cut by the disassembly task, to the outside, and a third transfer trolley configured to carry out a tray on which the battery cells are placed to the outside. The index table, the worktable, the second transfer trolley, and the third transfer trolley are arranged directly below the transfer rail.

The invention discloses a genome sequencing sample processing method based on a microdissection technology. The method comprises the following steps: (1) preparing a paraffin section or a frozen section; (2) carrying out HE staining on the section obtained in the step (1); (3) carrying out laser microdissection on nucleic acid; (4) fragmenting the DNA sample, and treating a product obtained in the step (3) by utilizing an ultrasonication method to obtain a DNA fragment; a non-contact ultrasonic crusher is adopted, the power is 80%, ultrasonic treatment is carried out for 10 s, stopping is carried out for 20 s, and 15 cycles are carried out. And (5) carrying out on-machine pretreatment on the genome sequencing sample, wherein the treatment comprises tail end repair and joint connection treatment. According to the method disclosed by the invention, accurate cutting of the sample can be realized, a DNA sample is extracted from a very small amount of cells and a sample for sequencing is constructed, the sample can achieve the same or higher sequencing data quality as that of a traditional method, the usage amount of the cells is greatly reduced, and the repeatability of an experiment is improved.

The invention relates to an extraction and culture method of urine-derived stem cells, and belongs to the field of cell biology and regenerative medicine. The method comprises the following steps: coating a culture plate with a gelatin solution; collecting clean midstream urine of healthy people and adding antibiotics; centrifugally separating the cell precipitate; washing with a phosphate buffer solution; re-suspending the precipitate by using a urine stem cell complete culture medium for primary culture; and after the cells grow to a certain fusion degree, carrying out passage amplification. The urine stem cell complete medium comprises a basic medium, fetal calf serum, epidermal growth factors, platelet-derived growth factors and basic fibroblast growth factors. By adopting the method disclosed by the invention, the urine-derived stem cells can be efficiently and stably separated from urine, and the obtained cells have high multiplication capacity, good stemness maintaining capacity, multidirectional differentiation potential and immunoregulation function, and can provide an ideal seed cell source for treating diseases such as diabetic erectile dysfunction.

The invention belongs to the technical field of antibody preparation, and particularly relates to an anti-CD38 nano antibody as well as a preparation method and application thereof. The method comprises the following steps: constructing and expressing CD38 antibody protein, immunizing alpaca, obtaining immune alpaca PBMC (peripheral blood mononuclear cells), extracting RNA (ribonucleic acid) of the cells, carrying out reverse transcription to obtain cDNA (complementary deoxyribonucleic acid), carrying out PCR (polymerase chain reaction) to obtain antibody gene segments, constructing the antibody gene segments on a phage expression vector to construct a CD38 immune antibody library, carrying out two rounds of panning, carrying out Clone ELISA (enzyme-linked immuno sorbent assay) detection on clones to obtain an antibody combined with the CD38 protein, carrying out sequencing analysis, and selecting a unique sequence. High-throughput expression is carried out through a mammalian cell high-throughput expression system, antibody protein is obtained, FACS detection is carried out through a CD38 high-expression cell line, and finally the antibody combined with CD38 is obtained. The method is simple and rapid to operate, a mammalian cell expression system induces efficient expression of the antibody, processing modification can be performed after translation, and the activity of the antibody is closer to that of a natural antibody.

The invention discloses a method for extracting primary hepatocyte lipid of an ICR mouse. The method comprises the following steps: digesting and extracting hepatocytes by using a buffer working solution 2 containing type IV collagenase by using a two-step collagenase perfusion method, then laying rat tail collagen in a pore plate, inoculating the extracted hepatocytes on the pore plate, culturing the cells, and finally extracting lipid from the cultured cells. The method is oriented to the ICR mouse, the problem that a primary hepatocyte extraction and culture system is unstable is solved, the cell extraction time is greatly shortened, the survival rate and the cell activity of the cells are improved, and the ICR mouse primary hepatocyte model is more effectively applied to lipidomics research.

The invention discloses a ship declaration cooperative processing system and method based on a multi-user role, and relates to the technical field of ship declaration, and the method comprises the steps: instantiating a digital twin software object for a new ship declaration request; extracting core semantic assertions, metadata and semantic potential energy parameters, packaging the core semantic assertions, the metadata and the semantic potential energy parameters into a semantic cell data unit, traversing all semantic cells in the digital twin, extracting the core assertions of the semantic cells, carrying out semantic matching on the core assertions and nodes in the domain knowledge graph, finding out a corresponding knowledge graph standard concept node, determining an acting force rule, and carrying out semantic analysis on the acting force rule; all semantic cells are placed in a semantic field, and each cell is endowed with a charge attribute obtained through mapping of the knowledge graph according to the core assertion of the cell; and the system runs a physical simulation cycle until the system reaches a steady state or a quasi-steady state, the total potential energy of the whole cell system is calculated, the total potential energy of the system is compared with a preset threshold interval through a normalization function and a calculated semantic stability score, and a decision branch is automatically triggered.

The present invention is related to the field of tissue regeneration. It concerns more particularly new processes, tubes and devices for thrombin, platelet concentrate and wound healant preparations, alone or in combination with cell extracts, cell compositions and uses thereof.

The application relates to the technical field of biological sample pretreatment, and discloses a high-efficiency separation method of a tissue cell extraction device. The method comprises the following steps: performing parameter calibration and reference loss modeling of a driving motor, and eliminating background interference of an actuator; applying a disturbance signal to a dissociation chamber, locking the characteristic resonance frequency of a tissue sample through frequency domain energy analysis, and performing pulsating crushing to accelerate the dissociation of a matrix structure; extracting the net torque of the motor in real time, deducing the fluid dynamic viscosity, adaptively adjusting the motor speed according to the viscosity drop trend, maintaining the fluid shear force in a preset target interval, and avoiding mechanical damage to cells; calculating the mechanical energy dissipation power in real time, and performing feedforward temperature control compensation to eliminate temperature overshoot caused by thermal conduction lag. The application realizes closed-loop control of the physical phase evolution of a heterogeneous tissue dissociation process, solves the limitation of a fixed parameter in a traditional method, shortens the dissociation time, and significantly improves the single-cell yield and survival rate.

This invention relates to the field of battery processing technology, and in particular to a multi-station blade battery cutting and core extraction device, comprising an integral processing frame. A cutting unit, a cross-cutting unit, and a core extraction unit are respectively mounted and connected on the upper surface of the integral processing frame. A material transfer bracket is fixedly mounted on the upper surface of the integral processing frame. Parallel linkage between the units is achieved through a linear slide module. During longitudinal cutting, the material transfer mechanism is synchronously ready and immediately performs the material transfer action after cutting, without any downtime. The material transfer, clamping, cutting, and flipping actions of the cross-cutting unit are continuously connected. The core extraction unit and the cross-cutting unit seamlessly coordinate their posture adjustments. The entire process requires no manual intervention. From battery loading, positioning, and cutting to cell extraction and material diversion, a closed-loop operation is formed. Simultaneously, the timing of each executing component is precisely controllable, effectively shortening the cycle time of a single process, meeting the capacity requirements of large-scale mass production and recycling / dismantling, and significantly reducing labor costs and operational risks.

The invention provides a cell image registration method and system, electronic equipment and a storage medium. The cell image registration method comprises the following steps: acquiring cell contours in a cell image map and a space transcriptome data map; respectively determining a part of target cells based on the definition of the cell contour; extracting geometric centers of a part of target cells, and respectively forming a cell image two-dimensional point cloud set and a space transcriptome data graph two-dimensional point cloud set; and finally obtaining registration parameters. And registering the cell image map and the spatial transcriptome data map according to registration parameters. According to the method, the cell contours and the definition of the cell contours in the cell image map and the space transcriptome data map are obtained, then the two-dimensional point cloud sets are formed based on the geometric centers of part of target cells, point cloud registration algorithm processing is carried out on the two-dimensional point cloud sets, and finally the registration parameters are obtained to carry out registration on the cell image map and the space transcriptome data map. The defect that in-situ analysis cannot be carried out is overcome, and the accuracy of a space transcriptome sequencing technology in a space-time omics technology is improved.

The utility model relates to the technical field of stem cell filtration, and discloses filtering equipment for stem cell extraction, which comprises a feeding cavity, a filtering cavity and a storage cavity, and further comprises a detection part, a filtering part and a control part, the stirring part is arranged in the center of the top of the feeding cavity, the detection part is located on one side of the stirring part, and the bottom end of the stirring part extends into the feeding cavity; the vibration part is arranged in the filtering cavity in a penetrating mode, and the stirring part makes contact with the top end of the vibration part when rotating to work. By arranging the feeding pipe, the floating ball and other structures, material supplementing can be automatically carried out, the situation that a large amount of solution enters the filtering equipment at the same time is prevented, filtering can be carried out at a stable speed, and by arranging the impacting assembly, the filtering plate can be impacted and vibrated in the stirring process, so that the filtering efficiency is improved. The filtering plate is prevented from being blocked, so that the smooth proceeding of a filtering procedure is ensured, and the stability of the device is improved.

The invention discloses a mouse primary cardiac endothelial cell extraction method, and belongs to the technical field of biological cell extraction. The method comprises the following steps: step 1, obtaining pure myocardial tissues; 2, the treated heart tissue is transferred into an intelligent tissue grinding system to be ground; step 3, transferring the ground heart tissue into a temperature-controlled enzymolysis reactor, automatically monitoring the digestion process, and terminating the reaction when a preset digestion degree is reached; step 4, carrying out primary cell separation, carrying out magnetic separation by using anti-CD31 magnetic beads, and specifically enriching heart endothelial cells; 5, transferring the purified endothelial cells to an intelligent microenvironment culture system, and automatically optimizing a culture medium replacement period to adapt to a cell metabolism state; and step 6, performing multi-dimensional quality evaluation on the endothelial cells, wherein the multi-dimensional quality evaluation comprises marker expression analysis, in-vitro lumen formation experiment, AI-assisted morphological analysis and digital quality scoring.

To provide systems and methods for histotripsy and immune therapies.SOLUTION: In some embodiments, histotripsy can be applied to a target tissue volume to lyse and solubilize the target tissue volume to release tumor antigens. In some embodiments, an immune response of the treatment can be evaluated. In other embodiments, an immune therapy can be applied after applying the histotripsy. In one embodiment, the lysed and solubilized cells can be extracted from the tissue. The extracted cells can be used to create immune therapies, including vaccines.SELECTED DRAWING: Figure 1

This invention discloses a multi-enzyme preparation for industrial cell dissociation, its usage method, and applications, belonging to the field of seed cell extraction technology for industrial meat culture. The invention employs a multi-enzyme preparation composed of component 1 and component 2; component 1 includes neutral protease, hyaluronidase, and elastase; component 2 includes matrix metalloproteinase, papain, and plasmin. The invention also discloses the preparation method and applications of the multi-enzyme preparation. The rational enzyme ratio and optimized treatment conditions of this invention help protect cell membrane integrity, maintain high cell activity, and significantly improve dissociation efficiency and cell recovery. The compound enzyme preparation is highly adaptable, applicable to various tissue types and dissociation requirements, and facilitates standardized and large-scale production. Combined with automated dissociation equipment, the compound enzyme preparation enables efficient and stable industrial operation, providing important technical support and broad application prospects for the cultured meat industry, tissue engineering, and cell therapy.

The utility model discloses an immune cell extraction structure, and relates to the related technical field of immune cell extraction auxiliary devices, the immune cell extraction structure comprises a lifting disc and extraction test tubes, a plurality of limiting holes are arranged on the lifting disc, the limiting holes are uniformly arranged on the lifting disc by taking the center of the lifting disc as an axis, and the plurality of extraction test tubes are in one-to-one correspondence with the limiting holes. The extraction test tube penetrates through the limiting hole and then is arranged in the limiting hole, a plurality of through holes are formed in the limiting hole, the through holes are evenly distributed in the side wall of the limiting hole with the extraction test tube as the center, pipe clamping pieces are further arranged on the lower end face of the lifting disc, a plurality of pipe clamping pieces form a group and evenly surround the limiting hole, and the pipe clamping pieces correspond to the through holes in a one-to-one mode. The tube clamping piece is rotationally connected with the lower end surface of the lifting disc; the extraction test tube is clamped with the lifting disc through the tube clamping piece. The device has the beneficial effects that the device can integrally move after centrifugal separation, so that the problems of pollution to immune cells and the like are reduced, and the purpose of improving the quality of the immune cells is achieved.

The utility model discloses a primary cell extraction device, which relates to the technical field of cell extraction, and comprises a cell centrifugal machine and a collecting device, the bottom of the cell centrifugal machine is fixedly connected with the top of the collecting device, in the utility model, the device can automatically cut and crush a cell sample through the reverse rotation of a left group of cutters and a right group of cutters; manual intervention is greatly reduced, the cell extraction efficiency is improved, meanwhile, the crushing consistency of a packaged sample can be achieved, the purity and yield of subsequent cell extraction are improved, a cell centrifugal machine is started according to a preset separation program for primary cells, the centrifugal process is automatically completed, layering of the cells and impurities is achieved, and after centrifugation is finished, the cell extraction efficiency is improved. Separated cells and supernate can be accurately collected into corresponding containers through the collection device, tissue smashing, cell separation and collection are integrated in one device, the sample transfer frequency is reduced, the pollution risk is reduced, and the working efficiency is improved.

1. Name of the design product: cell extraction equipment. 2. Use of the design product: for cell extraction. 3. Design points of the design product: in shape. 4. Picture or photo best indicating the design points: perspective view.

The utility model relates to the technical field of cell extraction devices, and discloses a sterile sampler for cell culture, which comprises a supporting base, a supporting rod is fixedly assembled on the top of the supporting base, a supporting ring is fixedly assembled on the outer wall of the supporting rod, a rotating seat is rotatably connected to the outer wall of the supporting rod, and a rotating shaft is fixedly assembled on the rotating seat. An operating rod is mounted at the top of the rotating seat, a supporting frame is fixedly assembled at the top of the rotating seat, and a pipetting device is mounted in an inner cavity of the supporting frame. According to the cell extraction device, the rotating seat is arranged on the outer wall of the supporting rod, the lifting plate is arranged on the outer wall of the rotating seat, a culture dish is placed on the lifting plate, and the lifting plate and the pipetting device are located on the same vertical line, so that even after the pipetting device obtains cell sap, the cell sap can drop from the bottom of the pipetting device, and the cell sap can be extracted from the culture dish; at the moment, the cell sap can fall into the culture dish again and cannot fall into other places, so that the influence on the operation environment is avoided.

A bioprocessing system for protein manufacturing from human blood is provided that is compact, integrated and suited for on-demand production and delivery of therapeutic proteins to patients. The patient's own blood can be used as the source of cell extracts for the production of the therapeutic proteins.

This invention provides a method for processing live single-cell extraction based on deformation trends, comprising the following steps: Step S1: Controlling a microfluidic chip to immerse itself in a culture medium containing adherent cells while maintaining a preset gap, controlling the injection flow rate of the injection channel and the aspiration flow rate of the aspiration channel to form a microjet acting on the cells; Step S2: Controlling an image processing module to receive and process cell morphology images on the culture substrate acquired by a microscopic imaging module, and calculating deformation parameters based on the comparison between the cell area in the current frame image and the initial cell area; Step S3: Comparing the deformation parameters with critical deformation parameters obtained through experimental calibration, and when the deformation parameters are greater than the critical deformation parameters, controlling a fluid drive module to reduce the injection flow rate and / or the aspiration flow rate; or, calculating a comprehensive damage risk index based on the deformation parameters, and when it is greater than 1, reducing the injection flow rate and / or the aspiration flow rate.

The application discloses an enhanced red blood cell lysis solution, a red blood cell DNA extraction kit and a method. The application provides an enhanced red blood cell lysis solution, which can improve the yield of red blood cell DNA. The application also provides a red blood cell DNA extraction kit and discloses a method for separating or extracting DNA from red blood cells. In addition, the application also discloses applications of the kit and the method for separating or extracting DNA from red blood cells, and the applications include applications in detecting cancer or predicting the risk of suffering from cancer. The enhanced red blood cell lysis solution and the kit provided by the application have important scientific value and application prospect.

This invention provides a method for determining the stacked cell hierarchy based on contour completion and gradient quantization. The method involves extracting the visible contour point set of each cell from a cell microscopic image; performing ellipse fitting on the visible contour point set of each cell to obtain the fitted ellipse parameters for each cell, including center coordinates, major axis length, minor axis length, and rotation angle; calculating a global gradient magnitude map on the cell microscopic image; filtering candidate cell pairs based on the fitted ellipse parameters, including overlapping cell pairs and adjacent cell pairs; for each cell pair in the candidate cell pairs, extracting the contour points of each cell within the overlapping region, obtaining the gradient values ​​corresponding to the contour points from the global gradient magnitude map, and calculating the average gradient value for each; determining the hierarchy relationship between the two cells by comparing the average gradient values; constructing an attribute graph using each cell as a node and the hierarchy relationship as an edge; performing topological sorting on the attribute graph; and outputting a global stacked hierarchy sequence.

The utility model relates to a multifunctional thyroid puncture sampling needle which comprises an outer needle and an inner needle, the outer needle and the inner needle are hollow, the inner needle can be driven to slide in the extending direction of the outer needle, a cell extraction part is arranged at the tip end of the inner needle, and a tissue extraction part is arranged on the side wall of the inner needle. An adjusting part for adjusting the size of an extraction notch of the tissue extraction part is arranged on the inner needle, when the inner needle is driven to extend outwards along the outer needle, the cell extraction part extends out of the end part of the outer needle to extract a cell sample, and the tissue extraction part cuts the tissue sample along with the sliding of the inner needle. According to the multifunctional thyroid puncture sampling needle disclosed by the utility model, the tissue sampling part is arranged on the side wall of the inner needle, and meanwhile, the cell extraction part is arranged on the tip end of the inner needle for respectively collecting cells and tissue samples, so that histology and cytology evaluation can be simultaneously carried out; meanwhile, the cavity between the inner needle and the outer needle is used for storing samples, so that the pollution risk and the operation complexity are reduced.

The invention discloses a preparation method and application of a leukocyte-inducing extract. The preparation method comprises the following steps: acquiring mesenchymal stem cells; inducing the mesenchymal stem cells to differentiate to generate dendritic cells; splitting the dendritic cells, and separating to obtain a cell extract; the differentiation comprises the steps of inducing the mesenchymal stem cells to differentiate into immature dendritic cells through the first induction composition and inducing the immature dendritic cells to mature through the second induction composition. The preparation method provided by the embodiment of the invention finds a new way, starts from the mesenchymal stem cells, and utilizes the characteristic of wide distribution of the mesenchymal stem cells to solve the problems of single source and difficult acquisition of leukocytes in the production process of the leukocyte extract; and moreover, the mesenchymal stem cells are differentiated, so that the situation that monocytes obtained by directly separating the albuginea layer are mixed into inflammatory cells is avoided.