343 results about "Cell extraction" patented technology

A method is provided for comparing multiple samples of cell extract containing a plurality of components. The method comprises the steps of preparing at least two samples of cell extract from at least two groups of cells and of exposing each of said sample of said cell extract to a different one of a set of matched markers, e.g. luminescent markers, to bind the marker to the cell extract to label the cell extract, each marker within said set of markers being capable of binding to the cell extract and can be individually detected from all other markers within said set. The samples are then mixed to form a mixture and said mixture is electrophoresed to separate the components within the cell extract. At least two electronic images of the electrophoresed mixture are obtained (I) by detection of the individual markers, each image being represented by detection of a marker different from the others. One resultant electronic image (I_res) of the obtained at least two electronic images is created (II) and analyzed in order to identify spot analysis areas (III). The identified spot analysis areas are applied on the respective at least two electronic images for evaluating said areas in order to detect spots representing components of said cell extracts (IV).

The invention discloses a three-dimensional porous cartilage extracellular matrix sponge scaffold made of natural cartilage, which can compound cells further to construct tissue engineered cartilage, and can be used for clinically repairing cartilage defects. In the invention, conditions which can fully perform cell extraction and form a porous scaffold matrix are provided by processing cartilage into cartilage microfilaments, and then the cell extraction and solidification and/or strengthening treatment are performed to obtain the three-dimensional porous cartilage extracellular matrix sponge scaffold which is completely decellurized. The antigenicity and cell components are removed in the natural cartilage, and an extracellular matrix component of the cartilage is retained to obtain the scaffold with appropriate pore diameter and porosity, suitable degradation rate, good biocompatibility and certain biomechanical strength. The scaffold has the advantages of broad material sources, low cost, simple and feasible preparation technology, and good repetitiveness; and the scaffold can be widely applied in the field of tissue engineering and has good clinical application prospect.

The invention describes methods and agents for improving cosmetic appearance, for promoting, improving or restoring health of cells and tissues, preferably skin, and more preferably, for restoring aged or damaged skin to a healthy appearance. This invention relates to the use of cells and cellular extracts in rejuvenation and healing technologies thereby improving healing and regeneration of all bodily tissues and organs. The present invention relates to compositions and methods of managing, preventing, and treating scars. The invention also relates to prevention of deterioration, damage and malfunction of cells and tissues, and to promote, improve or exceed cellular function in order to promote, improve and exceed appearance, vitality and health. In some embodiments, the invention relates to compositions of cells, eggs, cell extracts, egg extracts, and extract components such as purified nucleic acids, polypeptides, lipids, carbohydrates or other natural products. Said components can also be synthesized. In other embodiments, the cells are differentiable cells preferably stem cells or eggs. In some embodiments, compositions are used in a method that comprises application of compositions to tissues, including skin and/or wounds after removal of tissue surface layers. In other embodiments, the invention relates to methods of dedifferentiation of cells and/or dedifferentiation followed by differentiation. In other embodiments, the invention relates to managing, preventing, and treating skin diseases.

The invention relates to a blood vessel matrix for removing cell components in vascular tissue and a preparing method thereof. The invention provides a method which can remove the cell components in the vascular tissue and protect the structural integrity of the cell matrix at utmost. The method comprises the steps of cell extraction, detergent removing and enzyme treatment. The blood vessel cell removing matrix prepared by the invention has the advantages of excellent mechanical strength, proper porosity and low cytotoxicity and can successfully implant cells into the cell matrix, thereby providing a favorable tissue stent for the blood vessel tissue engineering.

The invention relates to genomically recoded organisms, platforms for preparing sequence defined biopolymers in vitro comprising a cellular extract from a genomically recorded organism, and methods for preparing sequence defined biopolymers in vitro are described. In particular, the invention relates to genomically recoded organisms comprising a strain deficient in release factor 1 (RF-1) or a genetic homolog thereof and at least one of at least one additional genetic knock-out mutation, at least one additional upregulated gene product, or both at least one additional knock-out mutation and at least one additional upregulated gene product.

The invention discloses a method for simultaneously treating multiple kinds of biological tissue. The method includes the steps of pretreating, degreasing, cell extraction, crosslinking and sterilization. The method for simultaneously treating the multiple kinds of biological tissue can simultaneously treat the multiple kinds of biological tissue, the batch-treated biological tissue amount is large, the treatment degree is uniform, the process is simple, industrialization is easy, and efficient production is achieved. According to the method for simultaneously treating the multiple kinds of biological tissue, use of strong acid, strong base and protease is completely avoided, natural three-dimensional collagen support fiber structures of extracellular matrixes of the multiple kinds of biological tissue can be reserved, and cell components, causing immunogenicity, in the tissue are removed. The biological tissue prepared through the method has good bio-compatibility and mechanical performance, compared with natural biological tissue, high degradation resistance is achieved, and the biological tissue can be used for repairing various kinds of soft tissue injuries.

The invention discloses an optimized in vitro cell-free protein synthesis system. The system comprises a cell extract, a carbohydrate material, a phosphate compound, a buffering agent and a DNA molecular template for encoding an exogenous protein, wherein the cell extract is a yeast cell extract inserted into a T7 RNA polymerase gene; the carbohydrate material is a mixture of glucose and maltodextrin; the buffering agent is a trihydroxymethyl aminomethane buffering agent; and the DNA molecule template is prepared by a nucleic acid isothermal amplification method, and a sequence as shown in SEQID NO.1 is inserted into the upstream of the coding sequence of the exogenous protein in the DNA molecular template. By optimizing, the cost of in vitro protein synthesis is reduced and the yield ofthe target protein is increased.

The invention provides an in vitro protein synthesis system, a kit and a preparation method. The in vitro protein synthesis system includes: (a) a cell extract; (b) an adsorbent used for enhancing protein synthesis efficiency; (c) amino acids; (d) nothing or NTPs; and (e) exogenous DNA molecules. A method for enhancing in vitro protein synthesis efficiency is provided. The method adopts the synthesis system and includes the following steps: (1) performing mixing; (2) adding the exogenous DNA molecules; (3) performing incubation synthesis; and (4) performing post-processing; and the method alsoincludes a step of adding the adsorbent after the step (1) or (2). In addition, the kit of the in vitro protein synthesis system is also provided. Therefore, the synthesis efficiency of foreign protein can be significantly enhanced.

The invention discloses a cell culture bottle. A clamping edge of a front panel of the cell culture bottle can be clamped and positioned between a clamping groove and a flanged edge of a back panel of another cell culture bottle; and thus, a plurality of culture bottles can be stored and moved in a piling mode, thereby saving the space of an incubator and facilitating the management. The joint of the cell culture bottle comprises two triangular plates and a trapezoidal plate, and the included angle formed by the trapezoidal plate and the front panel is 130 degrees; the lower side panel, bottom panel and upper side panel tilt towards the inner side of the bottle body, the bottleneck tilts towards the back panel, and a certain inclination angle is set; and when the cell culture bottle is arranged flatly, a pipette or cell scraper can touch all positions of the culture bottom surface, thereby facilitating the collection of cells, and preventing the culture bottle from generating dead area where cells can not be easily extracted. The invention solves the following problems: the plurality of cell culture bottles can not be easily managed and stored, the cell culture bottle generally has difficulty in cell extraction, and dead extraction areas can be easily generated.

A high suction double-cell extractor includes an outer cell defining an outer chamber, and an open-ended inner cell defining an inner chamber. The inner chamber is bottle-shaped and has a neck. The high suction double-cell extractor also includes a vertical loading system for applying axial force on a soil specimen during extraction. The high suction double-cell extractor also includes a port for introduction of pressurized air supply into the outer and inner cells. The introduced pressurized air can apply cell pressure on the soil specimen during extraction. The high suction double-cell extractor also includes a relative humidity control system and a differential pressure detector system. The high suction double-cell extractor can be used to measure stress-dependent soil-water characteristics curve (SDSWCC) under various stress states three-dimensionally and more accurately, and under total suction up to 8,000 kPa.

This invention provides a simple and convenient, single stage, single vessel cell extraction and assay method which is suitable for the extraction and measurement of a range of different types of analyte which occur as cellular components. The invention also provides kits of reagents suitable for performing cellular extraction and measurement as a single stage, single vessel process.

A orbital shaker for cell extraction includes a digestion chamber and structure for translating the digestion chamber. Structure is also provided for rotating the digestion chamber. A method of performing cell extraction is also disclosed.

Systems and methods are provided for producing a protein of interest that is typically not amenable to expression in soluble form in in vitro expression systems. In some aspects, the invention provides methods of synthesizing proteins using in vitro protein synthesis systems that include a scaffold protein such as apolipoprotein or an amphipathic alpha helix containing (“AAHC”) protein, in which higher yields of soluble protein are produced than in the absence of the scaffold protein. The scaffold proteins may be provided in an in vitro protein synthesis system associated with lipid or not associated with lipid. The scaffold protein may be provided as a protein per se or may be encoded by a nucleic acid template and co-expressed with the protein of interest. The invention also provides compositions and kits for synthesis of proteins in soluble form, in which the compositions and kits include cell extracts for protein expression and isolation.

The present invention discloses an in-vitro cell-free protein synthesis system. The system comprises cell extract, carbohydrate and a phosphate compound, wherein the carbohydrate is maltodextrin, lactose, or a combination of the maltodextrin and glucose, or a combination of the lactose and the glucose, or a combination of the maltodextrin, the lactose and the glucose. ATP is provided for an in-vitro reaction by low-cost substances such as the glucose, the maltodextrin and the lactose instead of energy sources such as phosphoenolpyruvic acid, phosphocreatine and acetyl phosphate, so that whilethe cost is reduced, a mode of providing energy by slow release prolongs the reaction time and increases the yield of target protein.

The present invention relates to a microcapsule biological feed, which is characterized in that the microcapsule biological feed comprises, by weight, 40-50 parts of composite high protein powder, 5-10 parts of an algae single cell extract, 20-30 parts of microbial protein yeast, 20-25 parts of detoxification fermentation potato powder, a microecology nutrient solution, and a feeding attraction film coating agent. The microcapsule biological feed of the present invention has advantages of fine product quality, high efficiency, safety, environmental protection, production cost reducing, and the like. In addition, the biological product is adopted to replace the chemical antibiotic additive so as to improve the food safety probability.

Disclosed are a form template definition method and a form template definition apparatus. The form template definition method comprises a cell extraction step of analyzing an image of a form so as to extract one or more cells from the image of the form; a cell classification step of classifying the extracted cells; and a cell attribute definition step of defining attributes of the extracted cells class by class. If an attribute of a first cell in one class is defined, then other cells in the class automatically copy the attribute of the first cell. As a result, the work amount of form template definition may be dramatically reduced by employing the form template definition method and form template definition apparatus.

The invention relates to the technical field of tissue engineering, in particular to a biofilm and a preparation method thereof. This method has simple process, low cost, less damage to the collagen fibers of membrane tissue, and good decellularization effect. The obtained biofilm retains the natural double-layer structure of animal membrane tissue and can guide tissue regeneration. The clinical application of the membrane has created conditions. The embodiment of the present invention provides a method for preparing a biofilm, comprising: step 1) removing the fatty tissue on the surface of the mammalian membrane tissue to obtain the membrane tissue after the preliminary degreasing; step 2) treating the membrane tissue after the preliminary degreasing with alkali solution soaking for decellularization to obtain decellularized membrane tissue; step 3) soaking the decellularized membrane tissue in an organic solvent, and performing degreasing treatment under vibration to obtain degreased membrane tissue; step 4) degreasing The final membrane tissue was flattened, dried, and sterilized to obtain a biofilm.

The invention provides a single-cell mass spectrometric analysis system and a single-cell mass spectrometric analysis implementation method. The system comprises a first micropositioner, a microscope,a second micropositioner, a first image collection component and a mass spectrometer which are sequentially arranged, wherein a cell locating chip is arranged on an objective table of the microscope;a liquid adding needle is arranged on the first micropositioner and is suitable for adding an extract liquor into the cell locating chip; and a liquid taking needle is arranged on the second micropositioner and is suitable for sucking extract obtained through extraction on the extract liquor and conveying the extract into the mass spectrometer. By use of the system, high-precision, high-speed, high-repeatability and high-automation mass spectrometric detection analysis on a single cell is realized, and the analysis quantity can reach a picoliter level. Meanwhile, inherent defects in the manual operation process can be overcome, mass spectrometric single-cell analysis gets rid of dependency on an operator, the process is more normalized, the system is easier and more convenient to use, analysis is more precise, and a result is more reliable.