123 results about "Cell extraction" patented technology

The present invention relates to a cartilage tissue single cell extraction kit and an extraction method thereof, the kit comprises: (1) a first enzyme for enzymatic hydrolysis, the first enzyme for enzymatic hydrolysis comprising one or more of trypsin, chondroitinase ABC and fucosidase; (2) a second enzyme for enzymolysis, wherein the second enzyme for enzymolysis comprises collagenase II, neutral protease II, hyaluronidase and Dnase I; and (3) a basic solution, wherein the basic solution comprises a basic culture medium and / or a buffer solution. The invention also provides a method for extracting single cells from cartilage tissues by using the kit. The cartilage tissue single-cell suspension prepared by the method has the advantages of high cell yield, high activity and the like, and has a wide application prospect.

The invention provides a lysosome extraction method. The lysosome extraction method comprises the following steps: (1) extracting cells; (2) tissue extraction; (3) crude separation of lysosome; and (4) separating the lysosome by magnetic beads. Through EDTA-free digestion optimization, lysis solution and buffer solution formula optimization, a filter membrane interception-magnetic bead purification combined technology, a double-antibody synergistic capture strategy and a competitive mild elution system, the four core problems of low purity, large activity damage, tedious operation and strong equipment dependence in a traditional lysosome separation technology are systematically solved. Experimental data show that the lysosome yield and functional integrity of the method are obviously superior to those of a density gradient centrifugation method, and the method is suitable for trace samples and conventional equipment and has high conversion value. In the future, the technical barrier can be further optimized through automation equipment integration and multi-dimensional function verification, and the application of the method in basic research and clinical diagnosis is promoted.

The system for dismantling the spent lithium-ion battery pack includes: a transfer section including transfer rails and a gantry robot on the transfer rails; a discharge processing section including an index table on which a discharging task of discharging a battery pack is performed; a battery disassembly section including a worktable on which a disassembly task is performed to cut connections of the battery pack, disassembly robots, and first transfer trolleys; and a cell extraction section including an extraction robot, a second transfer trolley configured to carry out battery cells, of which the connections have been cut by the disassembly task, to the outside, and a third transfer trolley configured to carry out a tray on which the battery cells are placed to the outside. The index table, the worktable, the second transfer trolley, and the third transfer trolley are arranged directly below the transfer rail.

The invention discloses a genome sequencing sample processing method based on a microdissection technology. The method comprises the following steps: (1) preparing a paraffin section or a frozen section; (2) carrying out HE staining on the section obtained in the step (1); (3) carrying out laser microdissection on nucleic acid; (4) fragmenting the DNA sample, and treating a product obtained in the step (3) by utilizing an ultrasonication method to obtain a DNA fragment; a non-contact ultrasonic crusher is adopted, the power is 80%, ultrasonic treatment is carried out for 10 s, stopping is carried out for 20 s, and 15 cycles are carried out. And (5) carrying out on-machine pretreatment on the genome sequencing sample, wherein the treatment comprises tail end repair and joint connection treatment. According to the method disclosed by the invention, accurate cutting of the sample can be realized, a DNA sample is extracted from a very small amount of cells and a sample for sequencing is constructed, the sample can achieve the same or higher sequencing data quality as that of a traditional method, the usage amount of the cells is greatly reduced, and the repeatability of an experiment is improved.

The invention relates to an extraction and culture method of urine-derived stem cells, and belongs to the field of cell biology and regenerative medicine. The method comprises the following steps: coating a culture plate with a gelatin solution; collecting clean midstream urine of healthy people and adding antibiotics; centrifugally separating the cell precipitate; washing with a phosphate buffer solution; re-suspending the precipitate by using a urine stem cell complete culture medium for primary culture; and after the cells grow to a certain fusion degree, carrying out passage amplification. The urine stem cell complete medium comprises a basic medium, fetal calf serum, epidermal growth factors, platelet-derived growth factors and basic fibroblast growth factors. By adopting the method disclosed by the invention, the urine-derived stem cells can be efficiently and stably separated from urine, and the obtained cells have high multiplication capacity, good stemness maintaining capacity, multidirectional differentiation potential and immunoregulation function, and can provide an ideal seed cell source for treating diseases such as diabetic erectile dysfunction.

The invention belongs to the technical field of antibody preparation, and particularly relates to an anti-CD38 nano antibody as well as a preparation method and application thereof. The method comprises the following steps: constructing and expressing CD38 antibody protein, immunizing alpaca, obtaining immune alpaca PBMC (peripheral blood mononuclear cells), extracting RNA (ribonucleic acid) of the cells, carrying out reverse transcription to obtain cDNA (complementary deoxyribonucleic acid), carrying out PCR (polymerase chain reaction) to obtain antibody gene segments, constructing the antibody gene segments on a phage expression vector to construct a CD38 immune antibody library, carrying out two rounds of panning, carrying out Clone ELISA (enzyme-linked immuno sorbent assay) detection on clones to obtain an antibody combined with the CD38 protein, carrying out sequencing analysis, and selecting a unique sequence. High-throughput expression is carried out through a mammalian cell high-throughput expression system, antibody protein is obtained, FACS detection is carried out through a CD38 high-expression cell line, and finally the antibody combined with CD38 is obtained. The method is simple and rapid to operate, a mammalian cell expression system induces efficient expression of the antibody, processing modification can be performed after translation, and the activity of the antibody is closer to that of a natural antibody.

The invention discloses a ship declaration cooperative processing system and method based on a multi-user role, and relates to the technical field of ship declaration, and the method comprises the steps: instantiating a digital twin software object for a new ship declaration request; extracting core semantic assertions, metadata and semantic potential energy parameters, packaging the core semantic assertions, the metadata and the semantic potential energy parameters into a semantic cell data unit, traversing all semantic cells in the digital twin, extracting the core assertions of the semantic cells, carrying out semantic matching on the core assertions and nodes in the domain knowledge graph, finding out a corresponding knowledge graph standard concept node, determining an acting force rule, and carrying out semantic analysis on the acting force rule; all semantic cells are placed in a semantic field, and each cell is endowed with a charge attribute obtained through mapping of the knowledge graph according to the core assertion of the cell; and the system runs a physical simulation cycle until the system reaches a steady state or a quasi-steady state, the total potential energy of the whole cell system is calculated, the total potential energy of the system is compared with a preset threshold interval through a normalization function and a calculated semantic stability score, and a decision branch is automatically triggered.

The present invention is related to the field of tissue regeneration. It concerns more particularly new processes, tubes and devices for thrombin, platelet concentrate and wound healant preparations, alone or in combination with cell extracts, cell compositions and uses thereof.

The application relates to the technical field of biological sample pretreatment, and discloses a high-efficiency separation method of a tissue cell extraction device. The method comprises the following steps: performing parameter calibration and reference loss modeling of a driving motor, and eliminating background interference of an actuator; applying a disturbance signal to a dissociation chamber, locking the characteristic resonance frequency of a tissue sample through frequency domain energy analysis, and performing pulsating crushing to accelerate the dissociation of a matrix structure; extracting the net torque of the motor in real time, deducing the fluid dynamic viscosity, adaptively adjusting the motor speed according to the viscosity drop trend, maintaining the fluid shear force in a preset target interval, and avoiding mechanical damage to cells; calculating the mechanical energy dissipation power in real time, and performing feedforward temperature control compensation to eliminate temperature overshoot caused by thermal conduction lag. The application realizes closed-loop control of the physical phase evolution of a heterogeneous tissue dissociation process, solves the limitation of a fixed parameter in a traditional method, shortens the dissociation time, and significantly improves the single-cell yield and survival rate.

This invention relates to the field of battery processing technology, and in particular to a multi-station blade battery cutting and core extraction device, comprising an integral processing frame. A cutting unit, a cross-cutting unit, and a core extraction unit are respectively mounted and connected on the upper surface of the integral processing frame. A material transfer bracket is fixedly mounted on the upper surface of the integral processing frame. Parallel linkage between the units is achieved through a linear slide module. During longitudinal cutting, the material transfer mechanism is synchronously ready and immediately performs the material transfer action after cutting, without any downtime. The material transfer, clamping, cutting, and flipping actions of the cross-cutting unit are continuously connected. The core extraction unit and the cross-cutting unit seamlessly coordinate their posture adjustments. The entire process requires no manual intervention. From battery loading, positioning, and cutting to cell extraction and material diversion, a closed-loop operation is formed. Simultaneously, the timing of each executing component is precisely controllable, effectively shortening the cycle time of a single process, meeting the capacity requirements of large-scale mass production and recycling / dismantling, and significantly reducing labor costs and operational risks.

To provide systems and methods for histotripsy and immune therapies.SOLUTION: In some embodiments, histotripsy can be applied to a target tissue volume to lyse and solubilize the target tissue volume to release tumor antigens. In some embodiments, an immune response of the treatment can be evaluated. In other embodiments, an immune therapy can be applied after applying the histotripsy. In one embodiment, the lysed and solubilized cells can be extracted from the tissue. The extracted cells can be used to create immune therapies, including vaccines.SELECTED DRAWING: Figure 1

This invention discloses a multi-enzyme preparation for industrial cell dissociation, its usage method, and applications, belonging to the field of seed cell extraction technology for industrial meat culture. The invention employs a multi-enzyme preparation composed of component 1 and component 2; component 1 includes neutral protease, hyaluronidase, and elastase; component 2 includes matrix metalloproteinase, papain, and plasmin. The invention also discloses the preparation method and applications of the multi-enzyme preparation. The rational enzyme ratio and optimized treatment conditions of this invention help protect cell membrane integrity, maintain high cell activity, and significantly improve dissociation efficiency and cell recovery. The compound enzyme preparation is highly adaptable, applicable to various tissue types and dissociation requirements, and facilitates standardized and large-scale production. Combined with automated dissociation equipment, the compound enzyme preparation enables efficient and stable industrial operation, providing important technical support and broad application prospects for the cultured meat industry, tissue engineering, and cell therapy.

The utility model discloses an immune cell extraction structure, and relates to the related technical field of immune cell extraction auxiliary devices, the immune cell extraction structure comprises a lifting disc and extraction test tubes, a plurality of limiting holes are arranged on the lifting disc, the limiting holes are uniformly arranged on the lifting disc by taking the center of the lifting disc as an axis, and the plurality of extraction test tubes are in one-to-one correspondence with the limiting holes. The extraction test tube penetrates through the limiting hole and then is arranged in the limiting hole, a plurality of through holes are formed in the limiting hole, the through holes are evenly distributed in the side wall of the limiting hole with the extraction test tube as the center, pipe clamping pieces are further arranged on the lower end face of the lifting disc, a plurality of pipe clamping pieces form a group and evenly surround the limiting hole, and the pipe clamping pieces correspond to the through holes in a one-to-one mode. The tube clamping piece is rotationally connected with the lower end surface of the lifting disc; the extraction test tube is clamped with the lifting disc through the tube clamping piece. The device has the beneficial effects that the device can integrally move after centrifugal separation, so that the problems of pollution to immune cells and the like are reduced, and the purpose of improving the quality of the immune cells is achieved.

The utility model discloses a primary cell extraction device, which relates to the technical field of cell extraction, and comprises a cell centrifugal machine and a collecting device, the bottom of the cell centrifugal machine is fixedly connected with the top of the collecting device, in the utility model, the device can automatically cut and crush a cell sample through the reverse rotation of a left group of cutters and a right group of cutters; manual intervention is greatly reduced, the cell extraction efficiency is improved, meanwhile, the crushing consistency of a packaged sample can be achieved, the purity and yield of subsequent cell extraction are improved, a cell centrifugal machine is started according to a preset separation program for primary cells, the centrifugal process is automatically completed, layering of the cells and impurities is achieved, and after centrifugation is finished, the cell extraction efficiency is improved. Separated cells and supernate can be accurately collected into corresponding containers through the collection device, tissue smashing, cell separation and collection are integrated in one device, the sample transfer frequency is reduced, the pollution risk is reduced, and the working efficiency is improved.

1. Name of the design product: cell extraction equipment. 2. Use of the design product: for cell extraction. 3. Design points of the design product: in shape. 4. Picture or photo best indicating the design points: perspective view.

The utility model relates to the technical field of cell extraction devices, and discloses a sterile sampler for cell culture, which comprises a supporting base, a supporting rod is fixedly assembled on the top of the supporting base, a supporting ring is fixedly assembled on the outer wall of the supporting rod, a rotating seat is rotatably connected to the outer wall of the supporting rod, and a rotating shaft is fixedly assembled on the rotating seat. An operating rod is mounted at the top of the rotating seat, a supporting frame is fixedly assembled at the top of the rotating seat, and a pipetting device is mounted in an inner cavity of the supporting frame. According to the cell extraction device, the rotating seat is arranged on the outer wall of the supporting rod, the lifting plate is arranged on the outer wall of the rotating seat, a culture dish is placed on the lifting plate, and the lifting plate and the pipetting device are located on the same vertical line, so that even after the pipetting device obtains cell sap, the cell sap can drop from the bottom of the pipetting device, and the cell sap can be extracted from the culture dish; at the moment, the cell sap can fall into the culture dish again and cannot fall into other places, so that the influence on the operation environment is avoided.

This invention provides a method for processing live single-cell extraction based on deformation trends, comprising the following steps: Step S1: Controlling a microfluidic chip to immerse itself in a culture medium containing adherent cells while maintaining a preset gap, controlling the injection flow rate of the injection channel and the aspiration flow rate of the aspiration channel to form a microjet acting on the cells; Step S2: Controlling an image processing module to receive and process cell morphology images on the culture substrate acquired by a microscopic imaging module, and calculating deformation parameters based on the comparison between the cell area in the current frame image and the initial cell area; Step S3: Comparing the deformation parameters with critical deformation parameters obtained through experimental calibration, and when the deformation parameters are greater than the critical deformation parameters, controlling a fluid drive module to reduce the injection flow rate and / or the aspiration flow rate; or, calculating a comprehensive damage risk index based on the deformation parameters, and when it is greater than 1, reducing the injection flow rate and / or the aspiration flow rate.

The application discloses an enhanced red blood cell lysis solution, a red blood cell DNA extraction kit and a method. The application provides an enhanced red blood cell lysis solution, which can improve the yield of red blood cell DNA. The application also provides a red blood cell DNA extraction kit and discloses a method for separating or extracting DNA from red blood cells. In addition, the application also discloses applications of the kit and the method for separating or extracting DNA from red blood cells, and the applications include applications in detecting cancer or predicting the risk of suffering from cancer. The enhanced red blood cell lysis solution and the kit provided by the application have important scientific value and application prospect.

This invention provides a method for determining the stacked cell hierarchy based on contour completion and gradient quantization. The method involves extracting the visible contour point set of each cell from a cell microscopic image; performing ellipse fitting on the visible contour point set of each cell to obtain the fitted ellipse parameters for each cell, including center coordinates, major axis length, minor axis length, and rotation angle; calculating a global gradient magnitude map on the cell microscopic image; filtering candidate cell pairs based on the fitted ellipse parameters, including overlapping cell pairs and adjacent cell pairs; for each cell pair in the candidate cell pairs, extracting the contour points of each cell within the overlapping region, obtaining the gradient values ​​corresponding to the contour points from the global gradient magnitude map, and calculating the average gradient value for each; determining the hierarchy relationship between the two cells by comparing the average gradient values; constructing an attribute graph using each cell as a node and the hierarchy relationship as an edge; performing topological sorting on the attribute graph; and outputting a global stacked hierarchy sequence.

The invention discloses a preparation method and application of an algae cell extract doped chitosan active composite membrane. Specifically, chitosan is adopted as a matrix, and two kinds of algal oil are introduced to enhance the functional performance of the membrane: one kind is that chlorella oil is directly doped into a chitosan solution, and the membrane is formed through a solution casting method, so that the mechanical performance and the antioxidant activity of the membrane can be effectively improved; and the other method comprises the following steps: combining schizochytrium limacinum oil with soybean protein isolate, preparing a stable Pickering emulsion by adopting an ultrasonic-assisted method, then mixing the stable Pickering emulsion with a chitosan matrix, and remarkably improving the homogeneity, the mechanical property and the functional stability of the membrane after the membrane is formed. The film material has good film-forming property, biodegradability and wide food fresh-keeping application potential.

The invention relates to the related technical field of cell extraction equipment, in particular to gene detection cell extraction equipment which comprises an extraction box, a box cover, a rotating shaft, a mounting disc and a control panel, a support is fixedly mounted on the bottom surface of the extraction box, an extraction cavity is formed in the extraction box, and the box cover is hinged to the extraction box; the rotating shaft is rotationally mounted at the bottom of the extraction cavity; a butt joint shaft with a positioning shaft mounting groove and a guide groove is arranged, a positioning shaft with a stress shaft and a guide seat is arranged in the positioning shaft mounting groove, and a supporting spring is connected to the guide seat, so that the positioning shaft is subjected to outward centrifugal acting force through operation of a rotating shaft, and the positioning shaft is made to move outwards; and when the rotating shaft stops running, the positioning shaft is automatically separated from the positioning groove under the action of the supporting spring, so that the convenience of replacing the mounting disc is effectively improved.

The invention relates to methods for extracting, purifying and concentrating mesenchymal stromal cell exosomes enriched with micro-RNA, proteins and lipids, and can be used in cosmetology and pharmacology for rehabilitation and regeneration. The technical result of the invention is a more efficient process for producing exosomes. This technical result is achieved in that the present method for extracting, purifying and concentrating exosomes from mesenchymal stromal cells includes cultivating cells, collecting the conditioned culture medium, depleting high-molecular-weight proteins, extracting, purifying and concentrating exosomes, and freezing and lyophilizing the exosome concentrate; in order to enrich the culture medium with exosomes, multipotent mesenchymal stem cells (MSCs) are grown in a medium having a lowered glucose concentration and containing platelet lysate and 5 mM of L-alanyl-L-glutamine, wherein, in the case of static cell cultivation, the growth medium is placed in a culture vessel and once a level of not less than 80% monolayer confluence is reached, the culture medium is harvested and replaced once every 24 hours, and in the case of flow-through cell cultivation, cells are placed on cell carriers in a bioreactor and 72-96 hours after the start of cultivation, the culture medium is harvested and replaced once every 24 hours, the exosome-enriched culture medium is subjected to concentration, which includes successive stages of centrifugation, tangential flow filtration and isopycnic centrifugation: in the first stage, the culture medium is centrifuged for 10 mins at 2000 g to remove large cell particles and other inclusions; in the tangential flow filtration stage, the supernatant is concentrated using hollow fibre filters having a molecular weight cutoff of 500 kDa, after which the concentrate is subjected to fivefold diafiltration with an equal volume of DPBS phosphate buffer solution using the same hollow fibre cartridge; and in the isopycnic centrifugation stage, the concentrated supernatant is mixed with a saccharose solution and deuterium oxide and subjected to isopycnic centrifugation at 100000 g and 4˚С for 75 mins, after which the concentrate is subjected to further fivefold diafiltration with a buffer solution, then the concentrate is filtered through filters with a pore diameter of 0.22 µm and 0.1 µm, the exosome concentrate is subjected to initial freezing to -75˚C in a freezing chamber, and drying is carried out in a lyophilization chamber, wherein in a first drying stage, the concentrate is cooled to -40˚С and subsequently held for 100 mins at an absolute pressure in the chamber of not more than 10 Pa, then cooled to -30˚С and subsequently held for 610 mins at an absolute pressure in the chamber of not more than 10 Pa, then cooled to -10˚С and subsequently held for 315 mins at an absolute pressure in the chamber of not more than 10 Pa, after which it is cooled to 0˚С and subsequently held for 130 mins at an absolute pressure in the chamber of not more than 10 Pa, then heated to +10˚С and subsequently held for 130 mins at an absolute pressure in the chamber of not more than 10 Pa; in a second drying stage, drying is carried out at +22˚С for not less than 130 minutes at an absolute pressure in the chamber of not more than 10 Pa.

The utility model discloses a feeder cell preparation operating table for a cataract mouse model, which comprises an operating table provided with a sliding hole and a mounting rod locked in the sliding hole through a nut, and further comprises a mounting piece elastically arranged on the mounting rod, the same spring piece is jointly installed at the center positions of the pair of rotating rods, one side of the spring piece is fixedly connected with the installation part, and the end of each rotating rod is sleeved with the same rubber belt so that the spring piece can release elastic potential energy to drive the pair of rotating rods to do circular motion around the rotating shaft to the bottom end to expand and tighten the rubber belt. Through cooperation of the mounting part, the mounting rod, the rotating rod, the spring piece and the rubber belt, the multi-purpose mouse cell extraction operation table is formed, the application range of the operation table is expanded, the operation accuracy is improved, meanwhile, injuries caused by mouse operation are reduced, the structural design is compact and reasonable, operation is convenient and fast, postoperative cleaning and disinfection are easy, and the operation table is worthy of popularization and application. And the practicability is high.

The invention discloses an exosome cell extraction device applied to oral soluble film production, relates to the technical field of exosome extraction, improves the phenomenon that the time for workers to find an extraction head and a test tube is prolonged due to the fact that the extraction head and the test tube which are independently packaged are adopted for extraction, and comprises a box body and an upper cover, a placing mechanism is movably arranged on the inner bottom wall of the box body, an extracting mechanism is arranged on the placing mechanism, the extracting mechanism comprises a test tube, a sealing plug, an extracting assembly, a dismounting and mounting assembly and a stabilizing assembly which are arranged on the placing mechanism, and the sealing plug is installed at the top end of the test tube in a sealed mode; the extraction assembly is arranged on the sealing plug in a penetrating mode and extends into the test tube, the disassembly and assembly assemblies are arranged on the edges of the two sides of the top end of the test tube, the lower portion of the test tube is sleeved with the stabilizing assembly, the situation that the extraction head accidentally moves in the test tube can be avoided, and the extraction precision is improved.

The invention belongs to the technical field of artificial skin preparation, and particularly relates to a system and method for preparing artificial skin through 3D printing, the system integrates key steps of cell extraction, amplification, artificial skin preparation and culture and the like, rapid amplification of skin cells and efficient construction of the artificial skin are achieved, the overall efficiency is remarkably improved, and the cost is reduced. The preparation period is shortened; the integrated square cabin system provided by the invention can move, so that the timeliness of cell extraction is ensured; besides, the prepared artificial skin with the net ridge structure has a certain thickness, the upper surface and the lower surface are flat, and the artificial skin has a wavy staggered three-dimensional structure, so that more epidermal stem cell dryness can be reserved, and the maintenance of epidermal stem cell marker protein is facilitated.

The invention provides a degradable polylactic acid-based human cell sampling brush wire material and a preparation method thereof, and relates to the technical field of preparation of medical supplies. The degradable polylactic acid-based human cell sampling brush wire material is prepared from the following raw materials: a composite modified material, polyethylene glycol, a plasticizer and an antibacterial agent, the composite modified material is obtained by mixing a product A prepared from polylactic acid and maleic anhydride and a product B prepared from polylactic acid and PBAT and then carrying out ultraviolet treatment, the plasticizer is one of glyceryl triacetate and acetyl tri-n-butyl citrate, and the antibacterial agent is chlorhexidine. The mechanical property of the prepared cell extraction brush wire material is improved while the existing characteristics of polylactic acid are not influenced, the elastic recovery rate is particularly improved, the cell extraction brush wire material has better elasticity and structural stability, and the structural stability and operability are kept when the cell extraction brush wire material is used for sampling positions such as cervix uteri; and the application value in cell extraction operation tools is comprehensively improved.

The utility model discloses a test tube placing rack for extracting and storing test cells, and relates to the technical field of cell extraction. The storage rack comprises a box body, a first storage rack body and a second storage rack body are placed on the upper side of the box body, the second storage rack body is located on one side of the first storage rack body, and L-shaped plates are fixedly matched with the two sides of the second storage rack body; the four square-shaped plates are in elastic and sliding fit with the upper side of the box body, and threaded rods are in running fit with the interiors of the square-shaped plates. Through the arrangement of the clamping plate, one end of the threaded rod drives one end of the clamping plate to slide in the square-shaped plate under the action of a worker, and one side of the L-shaped plate is fixed through the other end of the clamping plate, so that the problem that the second placing frame body shakes in the box body is reduced; and the fixing effect of the second placing frame body is improved.

The utility model provides a stem cell extraction constant-temperature culture device which comprises a main body mechanism and a culture mechanism arranged in the main body mechanism, the culture mechanism comprises a motor, a limiting box arranged at the output end of the motor, limiting gauzes arranged in the two sides of the limiting box, and a base arranged in the limiting box, the supporting blocks are arranged at the four corners of the top of the base, and the fixing pieces are arranged at the tops of the supporting blocks; when the stem cell culture box is used, after stem cells enter the culture box, the cover plate is rotated to enable the cover plate and the threaded ring at the top of the culture box to be screwed and fixed, the culture box is placed in the fixing piece and the limiting groove in the base, the base is placed in the limiting box for centralized culture, and meanwhile the motor drives the limiting box to rotate; meanwhile, when the stem cells are taken out, the culture box is independently taken out for taking the stem cells, and waste and pollution caused by excessive taking are prevented.

The utility model relates to a culture device for extracting stem cells, which is characterized by comprising a box body provided with a plurality of mounting shells, and stored samples can be inserted into the mounting shells. The installation shell is of a double-layer structure, and a heating pipe is spirally fixed in an interlayer of the installation shell. Circularly flowing liquid is poured into the heating pipe, and the liquid temperature is ensured to meet the expectation, so that the close-range layout of the heat source and the sample storage space is realized. According to the design, the heat loss is obviously reduced, the energy consumption is reduced, and the workload of the heating device is reduced. In addition, the structure can accelerate the heating process of the sample and ensure that the sample quickly reaches the required storage temperature.