946 results about "Flow cytometry" patented technology

A sample preparation instrument with an integrated device for estimating a concentration of white blood cells in a specimen is described. The sample preparation instrument receives a specimen for which a sample is to be prepared for analysis. The integrated device can implement an optical method, an electrical resistance method, or a flow cytometry method, for example, to estimate the white blood cell concentration in the specimen. For some types of analysis, a sample volume of the specimen is dependent on the white blood cell concentration. Therefore, the sample preparation instrument can automatically determine and adjust the sample volume of the specimen based on the estimated white blood cell concentration prior to adding additional reagents, such as labeling reagents and / or lytic reagents, to prepare the sample.

The invention belongs to the field of monoclonal antibodies, and particularly relates to a mouse anti-pelteobagrus fulvidraco IgM monoclonal antibody, a hybridoma cell strain secreting the monoclonal antibody and application, a CDR region of a heavy chain variable region of the mouse anti-pelteobagrus fulvidraco IgM monoclonal antibody comprises three sequences shown in SEQ ID NO.1-3, and a CDR region of a light chain variable region of the mouse anti-pelteobagrus fulvidraco IgM monoclonal antibody comprises three sequences shown in SEQ ID NO.4-6; the mouse anti-pelteobagrus fulvidraco IgM monoclonal antibody can be obtained by being secreted by a hybridoma cell strain PFM-1B7C12A1B2 with the preservation number of CCTCC (China Center for Type Culture Collection) NO: C2024234. The mouse anti-pelteobagrus fulvidraco IgM monoclonal antibody provided by the invention can be used for Western blot and ELISA specific detection of IgM in a pelteobagrus fulvidraco secretion form, and can be used for flow cytometry identification of pelteobagrus fulvidraco IgM + B cells. The monoclonal antibody is good in specificity, high in sensitivity and strong in affinity, can be used for structural analysis, immune response level detection and the like of pelteobagrus fulvidraco IgM, and lays a foundation for in-depth study of a pelteobagrus fulvidraco immune system and establishment of a vaccine immune effect evaluation method taking the antibody level as an index.

The invention provides a tumor detection data analysis method based on a peripheral blood immune marker, and belongs to the technical field of immunodetection.The tumor detection data analysis method comprises the steps that firstly, a peripheral blood sample of a patient is collected, and plasma and cell components are separated; analyzing various immune cell phenotypes by using flow cytometry; determining the concentration of key cell factors; analyzing the molecular expression level of the immune checkpoint; establishing an immune cell subset proportion empirical function; analyzing a cell factor expression mode by using an immune lineage transcriptome analysis model; establishing a tumor immune microenvironment score by combining the immune cell subset proportion and a model output result; the accuracy of the scoring system is verified through clinical follow-up visit and iconography examination; a standardized report template is formulated to provide a tumor early detection basis. Wherein the immune lineage transcriptome analytical model adopts a multi-modal attention fusion network architecture, three different immune data streams can be processed and integrated at the same time, and accurate evaluation of tumor-related immune states is realized.

The invention provides a sample pretreatment automation method for flow cytometry, and belongs to the technical field of sample pretreatment for flow cytometry. A biological sample to be detected is collected and placed in a collection tube containing an EDTA-K2 anticoagulant for pretreatment; an automatic pipetting system is used for carrying out incubation reaction on an anticoagulant whole blood sample and an intelligent matching fluorescence labeling antibody mixed solution, and a double-layer game optimization verification system is used for verifying the rationality of an antibody adding proportion and reaction condition parameters; carrying out fluorescence characteristic readability index prediction on the incubated cell suspension by adopting an intelligent dilution parameter regulator model, dynamically adjusting hemolysis parameters and suspension conditions, and adding 1 * hemolysin working solution by using an automatic liquid adding device to carry out hemolysis treatment so as to finish pretreatment; the technical problems that in the pretreatment process of flow cytometry detection samples, parameters are often based on artificial experience, and the reproducibility and standardization degree of detection results are insufficient are solved.

The invention discloses a monoclonal antibody BY017 combined with African swine fever virus P30 protein and application of the monoclonal antibody BY017. The invention provides an IgG antibody which is composed of a light chain and a heavy chain. CDR1, CDR2 and CDR3 in the heavy chain variable region are sequentially shown as the 47 to 54 , the 72 to 79 and the 118 to 126 in SEQ ID NO: 1; cDR1, CDR2 and CDR3 in a light chain variable region are sequentially as shown in the 49 to 60 , the 78 to 80 and the 117 to 124 in SEQ ID NO: 3. The invention also protects the application of any one of the IgG antibodies in preparation of products. The product has the functions that the product is used for being combined with an African swine fever virus P30 protein fragment; the antibody is used for binding African swine fever virus P30 protein; the antibody is used for binding African swine fever virus. The IgG antibody provided by the invention can be used for multiple purposes of western blot, immunohistochemistry, flow cytometry, ELISA (Enzyme-Linked Immunosorbent Assay) and the like, and can be used as an important tool for ASFV detection and structural analysis of P30 protein of ASFV detection.

The invention relates to the technical field of antibodies, and provides an anti-CD45 antibody for detecting a white blood cell common antigen based on flow cytometry and application of the anti-CD45 antibody, the antibody comprises at least one of the following CDRs: a heavy chain variable region CDR: an amino acid sequence of SEQ ID NO: 1-3 or a conservatively modified form thereof; and a light chain variable region CDR: SEQ ID NO: 4-6 or an amino acid sequence in a conservative modification form thereof. The antibody or the antigen binding fragment thereof can efficiently and specifically recognize CD45, and can effectively detect the CD45.

The invention discloses a method for improving plant polyploidy induction rate and obtaining polyploidy based on leaf bud primordium cell cycle synchronization. The method comprises the following steps: carrying out pre-differentiation culture on an explant induction material; performing cell cycle synchronization excitation treatment on the leaf bud primordium cells obtained by the pre-differentiation treatment; and performing chromosome doubling treatment on the explant induction material subjected to the cell cycle synchronization excitation treatment. According to the method disclosed by the invention, an EdU staining labeling method and a flow cytometry are used for accurately judging the time for carrying out cell cycle synchronization excitation treatment on leaf bud primordium cells of an induction material, and carrying out synchronization excitation and doubling induction on the induction material in time; the dosage of a cell cycle inhibitor for synchronous excitation treatment is accurate, the treatment opportunity is accurate, the treatment duration is proper, and the polyploidy inductivity of the plant is remarkably improved; the obtained polyploidy plant is good in stability, and the method is high in repeatability.

According to the sorting control system of the flow cytometry sorting instrument, liquid drop images are shot through a camera, and liquid drop states are quantitatively evaluated and monitored on the basis of image content information. In order to determine the liquid drop breaking state, a liquid drop image stroboscopic shooting mode is designed to observe the liquid drop state, and the STM32H7 provides a synchronous control signal. When the droplet generation state is stable, the droplet breaking image is close to a stationary state. The stroboscope lamp is controlled to be turned on at different moments in a period, so that different states of liquid drop generation can be observed. The time difference between the detection time and the charging time is quantitatively determined through liquid drop delay control, and accurate delay control over sorting is achieved. And finally, a full-automatic algorithm is adopted to calibrate the liquid drop time delay, the liquid drop time delay is continuously tried to be changed, and when the shooting brightness of the sorted CCD camera under a certain liquid drop time delay is the highest, the time delay is considered to be the current liquid drop time delay, so that full-automatic calibration of the liquid drop time delay is realized.

The invention discloses a verification method for regulating and controlling proliferation and differentiation of bovine adipocytes by gene interference, which comprises the following steps: cutting bovine adipocyte tissues into small blocks, directly pasting the small blocks in a culture dish for culture, and promoting cell growth and proliferation by using nutrition and growth factors of the tissues; a ZNF423 gene is targeted by adopting an siRNA technology, siRNA is transfected into bovine fat cells by utilizing a transfection reagent, and the siRNA is an interference fragment containing si-ZNF423; the method comprises the following steps: respectively extracting total RNA (Ribonucleic Acid) and total protein of bovine adipocytes, and detecting by utilizing qPCR (Quantitative Polymerase Chain Reaction) quantitative analysis, a WB (White Batch) technology, EdU dyeing, flow cytometry detection, oil red O, BODIPY (Boron Dipyrromethene), Nile Red dyeing, TG (Triglyceride) content determination and the like. According to the invention, ZNF423 gene expression is interfered by the interference fragment containing si-ZNF423, and the effect of inhibiting proliferation and differentiation of bovine adipocytes is detected by various methods, so that the effect of the interference fragment in the bovine adipocytes is known conveniently, and regulation and control of proliferation and differentiation of the bovine adipocytes are realized by interfering gene expression by the interference fragment. The beef quality can be improved; the beef cattle breeding can be optimized.

The invention discloses a flow-type Raman sorting method and a high-luminous-flux flow-type Raman sorting device, and the method comprises the following steps: carrying out coincident focusing on Raman laser and near-infrared laser captured by a light trap to form a Raman excitation detection site, and arranging the Raman excitation detection site at the tip of the outer end of a tail end electrode in a chip dielectric focusing electrode; the photo-thermal oscillation near-infrared laser is focused on a tail end electrode in a dielectric focusing electrode of the chip and does not coincide with a Raman excitation detection site; through switching of light trap capture near-infrared laser and photo-thermal oscillation near-infrared laser, a single cell sample is captured and fixed at a Raman excitation detection site through light trap capture force generated by the light trap capture near-infrared laser or bubbles are generated through a photo-thermal effect generated by the photo-thermal oscillation near-infrared laser; the cell sample adhered to the tail end electrode in the dielectric focusing electrode is oscillated and separated through the bubbles. Compared with the prior art, the device has the beneficial effects that the organic combination of optical trap capture and dielectric capture sample oscillation functions can be realized, the switching is convenient, flexible, rapid, simple and reliable in use, the instrument cost is greatly reduced, the application range of the instrument is effectively widened, and the device has very strong universality.

The invention relates to the technical field of biology, in particular to an anti-B7-H3 monoclonal antibody, a hybridoma cell strain and application thereof. The invention provides a monoclonal antibody. Amino acid sequences of three CDR regions of a heavy chain of the monoclonal antibody respectively have amino acid sequences shown as SEQ ID NO: 11, 12 and 13; the amino acid sequences of the three CDR regions of the light chain of the gene respectively have the amino acid sequences shown as SEQ ID NO: 6, 7 and 8. The anti-B7-H3 monoclonal antibody provided by the invention can be used for specifically recognizing a B7-H3 recombinant protein expressed by eukaryotic expression and a B7-H3 protein expressed on a cell membrane. A B7-H3 detection kit constructed by the anti-B7-H3 monoclonal antibody is high in sensitivity and good in specificity, and has a better application effect in various detections including enzyme-linked immunosorbent assay, flow cytometry, immunofluorescence and the like.

The invention provides a kit and a method for simultaneously detecting CEA, CA125 and CA153 based on a flow cytometry, which are characterized in that proper microspheres and antibody markers are specially arranged for CEA, CA125 and CA153 antibodies, a 1.5-step incubation method and a biotin system are combined, and proper blocking agents and confining liquid are added into a reaction buffer solution, so that the CA153 in a sample is better combined with the microspheres and the antibody markers, and the detection accuracy is improved. According to the present invention, the cross influence of the CA153 on the CEA and the CA125 is reduced, such that the detection accuracy can be significantly improved when the three tumor markers such as the CEA, the CA125 and the CA153 are simultaneously detected based on the flow cytometry, the detection sensitivity of the CEA can achieve 0.3 ng / mL, the detection sensitivity of the CA125 can achieve 0.5 U / mL, and the detection sensitivity of the CA153 can achieve 0.5 U / mL so as to meet the clinical requirements.

The invention provides an automatic sample distribution method for flow cytometry, which belongs to the technical field of flow cytometry and comprises the following steps: acquiring basic information of a sample; the method comprises the following steps: pre-analyzing a sample through a micro-fluidic chip to obtain sample density, sample viscosity and sample ion concentration; measuring the micro-channel diameter and the micro-channel length of the micro-fluidic chip by adopting a laser ranging system, and recording the characteristic parameters of the micro-channel material; a target distribution volume is set, a high-precision injection pump system is started for pre-injection, and an initial distribution rate is obtained through measurement of a flow sensor; calculating the initial distribution rate by using a fluid dynamic compensation equation set to obtain a corrected distribution rate; controlling the high-precision injection pump system to perform sample distribution according to the corrected distribution rate; performing real-time monitoring on a sample liquid level by using an ultrasonic sensor, and synchronously acquiring a real-time distribution rate; and sample shunting is performed through the micro-fluidic chip, so that multi-channel parallel distribution is realized.

The invention discloses a method for improving the synthesis level of L-tryptophan in escherichia coli based on ARTP mutagenesis high-throughput screening, and belongs to the technical field of bioengineering. According to the invention, the L-tryptophan biosensor pSensor-trp3 is constructed, and the L-tryptophan biosensor pSensor-trp3 is good in response under the condition that the exogenous addition concentration range of L-tryptophan is 0-300mg. L <-1 >. High-throughput screening is performed by using a flow cytometry, a dominant mutant strain YB-2 is obtained through iterative mutagenesis, and the shake flask L-tryptophan yield and the sugar-acid conversion rate of the dominant mutant strain YB-2 are 5.68 g.L <-1 > and 0.103 g.g <-1 > respectively and are increased by 11.4% and 10.7%. Through second-generation whole genome re-sequencing comparative analysis, it is speculated that the genes ynfB and waaO possibly have a promoting effect on synthesis of L-tryptophan. The L-tryptophan biosensor used in the invention also provides reference for high-throughput screening and transformation of other related L-tryptophan production strains.

The invention relates to the field of plant breeding, and particularly provides a method for fixing rice heterosis by using an OsZFPP gene. The method comprises the following steps: firstly, constructing an OsZFPP1 gene or OsZFPP2 gene expression cassette driven by an OsECA1 promoter; then, an expression box of rice with three target points of OsPAIR1, OsREC8 and OsOSD1 being knocked out of CRISPR / Cas9 is constructed; integrating the expression cassette into the same vector to transform hybrid rice; screening three-gene homozygous mutation and OsZFPP positive plants, and identifying diploid cloned offspring through flow cytometry and genome sequencing. According to the method, the rice OsZFPP gene is combined with MiMe to provide a novel method for fixing the heterosis of the rice through apomixis, the heterosis of the rice can be successfully fixed through the method, an apomixis system with the high maturing rate can be obtained, and a novel solution is provided for fixing the heterosis of the rice through the apomixis.

The invention discloses a chemical labeling system (HIM-IP) for detecting interaction between cells. According to the system, tyrosinase and phenol are respectively modified on the surfaces of two to-be-detected cells to construct capturing cells and prey cells, and then biotin or single-stranded DNA with nucleophilic groups is used as a molecular tag for labeling. The system not only can perform detection through traditional flow cytometry, but also can be compatible with a commercialized single cell transcriptome sequencing platform to realize association of intercellular interaction information and transcriptome information on a single cell level. The system does not need to carry out complex gene engineering modification on cells, the whole modification and marking process is simple, convenient and efficient, the detection sensitivity of intercellular interaction is high, and the biological mechanism of intercellular interaction regulation and control can be deeply excavated by integrating the single cell sequencing technology.

The monoclonal antibody of the glycoprotein Gn of the severe fever with thrombocytopenia syndrome virus is screened on the basis of immune repertoire sequencing, and the biological characteristics of the monoclonal antibody are identified. The method comprises the following steps: taking purified SFTSV-Gn as bait, taking virus specific B cells from an immunized female BALB / c mouse B cell bank, selecting an antibody sequence with potential binding activity by utilizing a 10 * immune repertoire sequencing method in combination with bioinformatics analysis, constructing the antibody sequence to an eukaryotic expression vector pcDNA3.4, expressing and purifying a monoclonal antibody, and identifying the biological activity of the monoclonal antibody. 14 antibodies are selected from 3544 captured B cells, functional verification shows that 12 antibodies can be combined with SFTSV-Gn glycoprotein, and the Gn-5 antibody and the Gn-7 antibody have high combining capacity; western blot, indirect immunofluorescence test and flow cytometry prove that the antibody can specifically recognize the SFTSV, the effective concentration is as low as 2 mu g / mL, and a foundation is laid for development of a rapid diagnosis reagent for the SFTSV.

The invention discloses a high-throughput antigen-specific T cell detection method based on mass spectrum flow cytometry. The invention belongs to the technical field of biology, and particularly relates to a high-throughput antigen-specific T cell detection method based on mass spectrum flow cytometry. According to the present invention, the activation induction marker (AIM) technology and the intracellular cytokine staining (ICS) are integrated, and the CyTOF platform is combined to achieve gt; 20 parameters are synchronously detected, and multiple activation-induction marker double positive markers, multifunctional cytokine spectrums (IFN-gamma, TNF-alpha, GZMB, IL-2 and the like) and immune memory phenotypes (CD45RA / CCR7) are covered. The standardized detection scheme provides a high-dimensional and quantifiable biomarker combination for vaccine protection efficacy evaluation, and has important clinical application value.

The invention discloses an auxiliary diagnosis system for renal transplantation rejection and typing thereof based on a peripheral blood immune cell map. Comprising a peripheral blood single cell acquisition unit, a mass spectrum flow cytometry unit, an original data preprocessing unit, a data conversion and normalization processing unit, a peripheral blood immune cell grouping acquisition unit, a peripheral blood immune cell map construction unit, a feature vector acquisition unit, a rejection prediction unit and a rejection typing prediction unit. According to the invention, a kidney transplantation two-stage diagnosis architecture is created for the first time; rejection detection and typing identification are realized through classification of an independently trained random forest binary classifier, and different TCMR / ABMR immune mechanisms are accurately matched; the traditional biopsy limitation is broken through, an auxiliary diagnosis scheme with high precision, low damage, moderate cost and typing identification capability is provided for the kidney transplantation rejection, and the method has important significance on timely discovery of the rejection of a kidney transplantation patient and improvement of prognosis.

A method of characterizing particles in flow cytometry includes determining a first pulse width value of a particle using a first technique. The method includes determining a second pulse width value of the particle using a second technique. The method further includes comparing the first and second pulse width values, and characterizing the particle as a concatenated particle when a difference between the first and second pulse width values exceeds a threshold.

The utility model relates to the technical field of cytometers, and discloses an automatic sampling mechanism of a flow cytometer, which comprises a bottom plate, a translation plate capable of horizontally moving is connected onto the bottom plate through a translation component, and a bearing component capable of rotating around the axis of the bearing component is connected onto the translation plate through a rotating component. A material loading part for clamping a test tube is mounted on the bearing part, and a uniform mixing assembly for uniformly mixing a sample in the test tube is also arranged on the bottom plate; the rotating assembly is mounted on the translation plate and can ensure that the bearing part can translate along a specified direction and can rotate around the axis of the bearing part, so that the material loading part can be automatically conveyed to the lower part of a corresponding sampling needle after clamping a test tube, and the interior of the test tube is mixed through the uniform mixing assembly; the operation precision and efficiency of the mechanism are both higher than those of manual operation, the labor intensity can be reduced, the tedious manual operation is liberated, the sample detection efficiency of flow cytometry is improved, and rapid sample loading and detection of the flow cytometry on samples are realized.

The invention discloses an anti-CD3D antibody and application thereof.The antibody comprises a heavy chain variable region and a light chain variable region, the amino acid sequence of CDR-H1 of the heavy chain variable region is shown as SEQ ID NO: 4, the amino acid sequence of CDR-H2 of the heavy chain variable region is shown as SEQ ID NO: 5, and the amino acid sequence of CDR-H3 of the heavy chain variable region is GDL; the amino acid sequence of the CDR-L1 of the light chain variable region is as shown in SEQ ID NO: 6, the amino acid sequence of the CDR-L2 of the light chain variable region is as shown in SEQ ID NO: 7, and the amino acid sequence of the CDR-L3 of the light chain variable region is as shown in SEQ ID NO: 8. The anti-CD3D antibody is a rabbit monoclonal antibody, the obtained antibody is high in titer, clear in specific positioning in immunohistochemical staining and free of non-specific background staining, the antibody is wide in application, and the antibody can be used in pathological immunohistochemical staining experiments and can also be used in Western blot and flow cytometry experiments.

The invention discloses an anti-soluble transferrin receptor monoclonal antibody as well as a preparation method and application thereof, and belongs to the technical field of antibody detection. According to the antibody, an L101-F760 amino acid sequence of human TfR protein is used as an immunogen, specific screening of B cells, reverse RNA (Ribonucleic Acid) into cDNA (Complementary Deoxyribose Nucleic Acid) through RT-PCR (Reverse Transcription-Polymerase Chain Reaction), then homologous recombination is performed to construct heavy and light chain vectors, and finally multiple rounds of screening are performed to obtain the purified sTfR monoclonal antibody, so that the sTfR protein can be specifically recognized, and the antibody has the advantages of high specificity and high sensitivity and can be widely applied to the field of immunotherapy. The natural sTfR protein in human serum can be recognized. The kit is applied to the detection and screening fields of immunohistochemistry, immunofluorescence chemistry, western blot, co-immunoprecipitation, indirect ELISA, flow cytometry and the like, especially has low background and no interference of non-specific binding impure protein during immunohistochemistry detection, can obtain more accurate detection and evaluation results, avoids interference of false positive and false negative, and has high detection accuracy. The method has great application value in the aspects of basic scientific research and clinical pathology detection.

The present invention relates to novel antibodies that bind to human CB2. Furthermore, the present invention relates to a method of detecting a change in cell surface CB2 expression in response to treatment with a CB2 agonist by flow cytometry. The methods allow for the assessment and monitoring of target conjugation of CB2 agonists, thereby supporting preclinical and clinical development of CB2 agonists.

The invention provides a multi-focus excitation confocal fluorescence microscopic imaging flow cytometry screening method. The method is characterized in that a laser beam generates three laser beams through a spatial light modulator, and the three laser beams are coherently superposed in a focal plane of a microscope objective to generate a two-dimensional optical lattice light field. And after the other laser beam is shaped by the cylindrical lens, linear laser optical tweezers are realized through the microscope objective, cells to be detected in an imaging detection area of the micro-flow chip are captured, and accurate active optical control on the rotation angle of the cells to be detected is realized. In a cell rotation process, a two-dimensional optical lattice light field rapidly scans in a focal plane at different angles, and a fluorophore in the cell is excited to generate a fluorescence signal. The confocal pinhole array installed at the position conjugate with the focal plane eliminates background fluorescence, a high-spatial-resolution fluorescence image of a three-dimensional structure in the cell is obtained, and cell screening is achieved. The method provided by the invention has the characteristics of high detection rate, high accuracy and the like, and has wide application prospects in the research fields of biology, medicine and life science.

SNAIL provides cost-efficient detection of specific nucleic acids in single cells, and may be combined with flow cytometry to simultaneously analyze large numbers of cells for a plurality of nucleic acids, e.g. at least one, to up to 5, up to 10, up to 15, up to 20 or more transcripts can be simultaneously analyzed, at a rate of up to about 50, 100, 250, 500 or more cells / second. The methods require only two primers for amplification, and may further include a detection primer.

The invention discloses a screening method for a riboswitch capable of specifically recognizing doxycycline as well as rational transformation and application of the riboswitch, and belongs to the field of gene expression regulation and control. The invention provides a doxycycline specific riboswitch, establishes a high-throughput screening method based on a flow cytometry, and obtains the doxycycline riboswitch with further improved activation multiple by means of computer-aided prediction of binding sites of the riboswitch and calculation of mutation sites. According to a dose reaction curve, the riboswitch shows linear correlation at 40-100 [mu] g / L, and meanwhile, the constructed whole-cell sensor is low in price, high in stability and simple to operate, and can be used for on-site rapid detection.

The invention discloses an African swine fever virus T cell epitope peptide for inducing swine T cell immune response and a composition of the African swine fever virus T cell epitope peptide. The amino acid sequence of the African swine fever virus T cell epitope peptide is shown as any one of SEQ ID NO.1-19. Or an amino acid sequence which is derived from the amino acid sequence shown in SEQ ID NO.1-19 and maintains the protein function shown in SEQ ID NO.1-19 through substitution and / or deletion and / or addition of one or more amino acid residues of the amino acid sequence shown in SEQ ID NO.1-19; the composition comprises amino acids of which the sequences are SEQ ID NO.1-19. A group of ASFV key T cell antigens and epitope peptides are obtained by combining IFN-gamma ELISpot and flow cytometry screening, the ASFV key T cell antigens and epitope peptides can be used as vaccine components to induce strong T cell immune response, and T cells activated by the antigen peptides have the maximum immune protection potential and are essential key antigens for ASFV vaccine research and development.

Provided are a fully human TSH receptor (TSHR) blocking monoclonal antibody for treating hyperthyroidism, and a preparation method therefor and a use thereof. The method comprises the following steps: using flow cytometry to sort plasma cells and memory single B cells specifically recognizing a TSHR in the peripheral blood of patients having high titers of TSHR blocking antibody (TSH-stimulating blocking antibody, TBAb), performing in vitro cloning of antibody light and heavy chains and performing recombinant expression, and using hTSHR-CHO cells for antibody property screening and validation, to obtain a blocking monoclonal antibody specifically targeting human TSHR. The fully human TSHR blocking monoclonal antibody can specifically bind to the TSHR, has good inhibitory activity in vitro and in vivo experiments, and has broad application prospects in the development of treatment of Graves' diseases.

The invention relates to the technical field of ovarian granule cell apoptosis, and discloses a method for regulating oxidative stress induced sheep ovarian granule cell apoptosis by using allicin, and the method comprises the following steps: firstly preparing a culture solution, then preparing an allicin stock solution, and identifying sheep ovarian granule cells; the method comprises the following steps: firstly, measuring the activity of ovarian granular cells after independent treatment of H2O2 and allicin, determining the optimal treatment time and concentration of H2O2 and allicin, then carrying out co-treatment of H2O2 and allicin, detecting the apoptosis of the granular cells by using flow cytometry, detecting the expression of apoptosis-related genes by using real-time fluorescent quantitative PCR, detecting the secretion of steroid hormones by using ELISA, and carrying out data analysis. The invention solves the problems that in the prior art, an effective regulation and control method for ovarian granular cell apoptosis induced by oxidative stress is lacked, and traditional medicines possibly have large side effects, unobvious effects and the like.