978 results about "Artificial insemination" patented technology

The present invention provides a method of cryopreserving sperm that have been selected for a specific characteristic. In a preferred embodiment, the method is employed to freeze sex-selected sperm. Although the cryopreservation method of the invention can be used to freeze sperm selected by any number of selection methods, selection using flow cytometry is preferred. The present invention also provides a frozen sperm sample that has been selected for a particular characteristic, such as sex-type. In preferred embodiments, the frozen sperm sample includes mammalian sperm, such as, for example, human, bovine, equine, porcine, ovine, elk, or bison sperm. The frozen selected sperm sample can be used in a variety of applications. In particular, the sample can be thawed and used for fertilization. Accordingly, the invention also includes a method of using the frozen selected sperm sample for artificial insemination or in vitro fertilization.

The invention discloses a frozen bovine semen diluent, and the consumption amounts of all the raw materials in 100ml of the diluent are: 0.8 to 1.2g of fructose, 1.4 to 1.6g of sodium citrate, 2.3 to 2.6g of TRIS, 7.5 to 9.5g of low-density lipoprotein, 5 to 8ml of glycerol, 0.085 to 0.12 million IU of penicillin and the rest is distilled water. The preparation method is that: the fructose, the sodium citrate and the TRIS are dissolved in the distilled water to be prepared into a base liquid; penicillin G sodium and the low-density lipoprotein are added in the base liquid to prepare a I liquid; the glycerol is added in the I liquid to prepare a II liquid; then the pH value is adjusted to 6 to 7.5, then a filtration and a sterilization are carried out, and the liquid is cooled until reaching the room temperature and then arranged in a refrigerator of 2 - 5 DEG C for standby. The frozen bovine semen diluent of the invention has good and reliable effect, which can provide high-quality and excellent straw frozen semen for bovine artificial insemination and have very broad market application prospect.

The invention discloses a simple, convenient and efficient fixed-point gene editing method relating to the fields of transgenic animal preparation, gene functional study and biomedicines. A testis injection method is a sperm-mediated gene transferring method developed by utilizing the sperm capacity of actively combining, transferring and integrating an exogenous DNA. By using the method disclosed by the invention, an animal testis injection method is optimized, a carrier constructed by utilizing a clustered regularly interspaced short palindromic repeat system (CRISPR/Cas9) is integrated with a sperm chromosome through multi-point testis injection or seminiferous tubule microinjection, so that the aims of deleting, replacing and inserting genes are achieved. Then, a transgenic animal descendant is obtained through various approaches such as natural mating and artificial insemination. According to the method, the advantages of a CRISPR/Cas9 gene editing system are sufficiently utilized, and the existing transgenic animal system is combined, so that the complexity of in-vitro cell operation and requirement on expensive precise instruments/equipment are avoided while the gene modification animal obtaining efficiency is greatly increased.

Synchronous induced spawning for parent fish better solves the problem of sexual maturity through synchronous development of female and male parent fish. The invention discloses an artificial propagation method for sturgeon, which comprises the steps of selection of parent fish, parent fish rearing, induced spawning, artificial insemination, zygote hatching, artificial selection of fish fry, and fish fry rearing. In the artificial propagation method, by artificially reducing water temperature and ambient environment temperature, micro water flow is formed to simulate the ecological environment for the reproduction of sturgeon, the simulated ecological environment is similar to the conditions necessary for the reproduction of wild sturgeon in the natural world, and the artificial low temperature factor lasts for a long time, so as to always guarantee the low temperature environment for the maturity and awaiting delivery of parent fish of the sturgeon, and meet other condition requirements, so that the parent fish can rut and spawn all year round to finally achieve the factory production of the sturgeon fry; meanwhile, in the artificial propagation method, female parent fish and male parent fish of the sturgeon are separately put into an artificially simulated ecological environment for inducing oestrus, and then the degree of sexual maturity of the parent fish is identified to select the parent fish with basically developed sexual maturity.

The invention relates to a diluent for preserving porcine semen at normal temperature. A total solute of the diluent comprises 45 to 55 percent by weight of monose combination, 15 to 25 percent by weight of amino acid combination and 25 to 35 percent by weight of electrolyte, buffer, antioxidant, stabilizing agent and antibiotics. Various substances are dissolved in double distilled water and a volume of 1, 000 milliliters is fixed so as to finally make an osmotic pressure of the diluent in the range of 330 to 360mosmo/L and make a pH value of the diluent in the range of 6.3 to 6.5. After theporcine semen is preserved at normal temperature in the environment of the diluent of the invention for 5 to 7 days, the viability can still be kept over 0.6 and the acrosom entire rate can reach 85 percent. When the porcine semen preserved by adopting the diluent of the invention is used for mating on 260 sows, the conception rate and the litter size reach 90.2 percent and 10.7 respectively and reach or exceed the levels of the import diluent. The diluent of the invention replaces the import bovine serum albumin with the monose combination and the amino acid combination, but the cost of the diluent of the invention is only 1/3 of that of the import diluent, so the production cost of artificial insemination of swines is reduced greatly.

The invention relates to an apparatus and a method useful for non-surgical embryo transfer or artifical insemination of mammals. An apparatus for depositing media into the uterus of a mammal includes a conical chamber that has a plurality of perforations and flaps. An exterior spiral formation is configured for traversing or penetrating cervical passageway. A sheath having a frusto-conical rearward end extends axially from an aft end of the conical chamber. A tubular depositing chamber extends axially from the conical chamber to a position beyond a fore end of the conical chamber. Coupled to the rearward end of the depositing chamber is an embryo or semen packaging unit. The tubular depositing chamber has an end that has an aperture to permit the flow of semen out of the depositing chamber and into the uterus. The invention also involves a method for depositing of embryos or semen into a uterus of a mammal by a) inserting a conical chamber having a fore end and an exterior spiral formation into a cervix of a mammal, b) securing the conical chamber with the walls of the cervix, c) projecting a depositing chamber through an interior portion of the conical chamber, d) moving the depositing chamber to an embryo or semen release position for release of embryos or semen in the uterus, and e) securing an embryo or semen packaging unit to the depositing chamber to deposit embryos or semen in the uterus.

The invention discloses a novel movable retaining frame for cattle. The two ends of a frame body are provided with a front rail door and a rear rail door respectively. The front rail door comprises a border, a neck cangue vertical column vertically arranged in the border and a transverse short column arranged on the side portion of the border, wherein the transverse short column is provided with a rotary shaft, the rotary shaft penetrates through a rotating rod, when the rotating rod rotates based on the rotary shaft which is taken as a rotation point, the positions, corresponding to the tracks of the two ends of the rotating rod are provided with an upper fixing groove and a lower fixing groove respectively, the rotating rod is provided with a positioning clamping groove, the border of the front rail door is connected with one end of a push-pull quick clamp, the push-pull quick clamp is fixedly connected with one end of a transmission connecting rod through a connecting column, and the other end of the transmission connecting rod is clamped in the positioning clamping groove and can move in a reciprocating mode along the positioning clamping groove. The novel movable retaining frame can be used for hoof renovation, artificial insemination, surgical operations, tissue sampling and other operations and meanwhile facilitates moving.

The invention relates to a technical method of artificial propagation of rainbow trout, belonging to the technical field of coldwater fish (salmon and trout) cultivation. The technical method comprises the following steps: step 1, preparing seepage of single salt and the like; step 2, implementing egg washing and artificial insemination of seepage of single salt and the like; step 3, implementing continuous operation of flowing water, absorbing water, expanding and incubation of oosperm in a mesh pot barrel-type incubator; step 4, detecting fertility rate in 4 cell stage; step 5, adopting a mesh cage (or gravel) flat flow type larval fish film-out incubation groove to implement larval fish film-out incubation; step 6, after the larval fish is incubated, removing the mesh cage or the gravel, carrying out larval fish opening training and raising in a primary groove; step 7, after one month of training and raising, removing the larval fish into a hexagonal raising groove, and carrying out offspring seed raising. By adopting continuous operation technology of flowing water, absorbing water, expanding and incubation, the technical method reduces operation procedures and operation tools of stopping water, absorbing water and expanding, and improves the incubation rate by 20%. Compared with the existing embryonic detection technology, the fertility rate detection technology in 4 cell stage is mastered 9 days in advance, thus avoiding the problem that embryonic detection is not accurate.

The invention relates to a livestock semen freezing diluent as well as a preparation method and application of the livestock semen freezing diluent. The preparation method of the livestock semen freezing diluent comprises the following steps of: adding 2.71 grams of trihydroxymethyl aminomethane, 1,4 grams of citric acids, 1.0 gram of monosaccharides, 10 myriad IU (International Unit) of penicillium, 10 myriad IU of streptomycin and 0.29-6.97 grams of inositol compounds to a right amount of ultrapure water; optionally adding 5-20 milliliters of penetrability protectants; uniformly stirring; regulating a pH value to 6.8-7.2; adding 5-20 milliliters of fresh yolk subjected to inactivation treatment; uniformly mixing, and then fixing the volume to 100 milliliters; centrifugalizing at low temperature for 1 hour; and filtering supernate to prepare the livestock semen freezing diluent. Because the inositol compounds can effectively inhibit the generation of ice crystals, the livestock semen freezing diluent disclosed by the invention reduces the mechanical injury caused by the ice crystals on semens, achieves the survival rate of the defrozen semens at about 75%, the activity more than 50%, the acrosome integrality at about 60% and the plasmalemma integrality at about 50% and achieves the non-return rate more than 70% after artificial insemination; and the livestock semen freezing diluent has the advantages of no immune response generation, purity guarantee, low price, and the like by using the inositol compounds as the ice crystal inhibitors of the semen freezing diluent.

The invention discloses a construction and stock breeding method for cultured grass carp families. The method comprises the following steps of: selecting grass carps which move actively and have high heritability and of which the weight is 15 to 20 percent more than the average weight, as parent fishes; marking cultured grass carps by using periodic interrupt timer (PIT) electronic labels, after sexual gland is matured, facilitating maturation by using hormone solution, performing artificial insemination on female and male parent fishes to obtain oosperm, performing self-hatching, then growing seedlings, breeding fishseed, breeding one-year fishes, feeding respectively, and establishing the families; from the second year to maturation time, injecting the PIT electronic label into the grass carps with the different families; performing mixed culture in a same pond, and eliminating the inferior grass carps, wherein the rest families are the basic families for next generation breeding; the young grass carps, the one-year grass carps, two-year grass carps, three-year grass carps and four-year grass carps are a critical period of family breeding; preferably choosing, and establishing the families serving as backup parent fishes; and then matching teams, raising the seedlings and then breeding continuously. A new product with high quality, high yield and stable yield is bred by establishing the different families of the cultured grass carps; and the method is used for researching the genetic characteristic and breed differentiation conditions by mating the male and female fishes among the grass carp families and inbreeding the male and female fishes in the grass carp family.

An intensive fry breeding system of Pelteobagrus fulvidraco includes a disease prevention and control region for fish body quarantine and sterilization of personnel, tools and water entering the system; a parent fish sorting region including at least one parent fish temporary culture pond for cleaning and specification sorting of parent fishes entering the system from outside; a parent fish spawning inducement region including at least one spawning inducement pond and at least one injection workbench for performing injection spawning inducement to parent fish; an artificial insemination region for completing artificial spawn collection, artificial insemination, artificial debonding and washing; a spawn hatching region including at least one set of fry hatching device for hatching spawn into fry; and a fry temporary culture region including at least one fry temporary culture pond for temporary culture and first-feeding, wherein the disease prevention and control region, the parent fish sorting region, the parent fish spawning inducement region, the artificial insemination region, the spawn hatching region and the fry temporary culture region are all arranged at the production site. The above functional regions are adjacent in sequence. The inventive system has novel facilities, industrialized and large-scale fry breeding and reduced fry propagation cost, and is small in land occupation and low in water consumption.

The invention relates to a seed producing method for obtaining the hybrid advantage of a crassostrea gigas, which is characterized by comprising the following steps: selecting Chinese and Korean crassostrea gigases as parent oysters to carry out temporary culture; ripening the Chinese and Korean crassostrea gigases synchronously; then hybridizing sperms and ovums of the Chinese and Korean crassostrea gigases by artificial insemination; and finally carrying out the incubation, the seed culture and the growth of the hybridized oyster. The invention cultures a crassostrea gigas hybridized seed with high disease resistance and survival rate and rapid growing speed by hybridizing two crassostrea gigas geographic populations with reproductive isolation; the production performance of the crassostrea gigas hybridized seed is obviously better than that of a Chinese locally inbred comparison population; and the method is simple and has favorable industrialized application prospect.

The invention discloses a special feed additive for conditioning the breeding performance of artificially inseminated breeder cocks and application thereof. The special feed additive for conditioning the breeding performance of artificially inseminated breeder cocks is prepared from a composite traditional Chinese herbal additive and a nutritional feed additive according to a weight ratio of 1:1, wherein the composite traditional Chinese herbal additive comprises 7-10 percent of epimedium, 7-10 percent of actinolite, 7-10 percent of yam, 7-10 percent of prepared rhizome of rehmanniat, 5-8 percent of medlar, 5-8 percent of cistanche salsa, 5-8 percent of dodder, 5-8 percent of raspberry, 5-8 percent of plantain seeds, 5-8 percent of japanese teasel, 5-8 percent of liriope, 3-4 percent of Dangshen, 3-4 percent of morinda officinalis, 3-4 percent of astragalus, 3-4 percent of angelica sinensis, 3-4 percent of atractylodes and 3-4 percent of glycyrrhiza, and the percent sum of the raw materials is 100 percent. The nutritional feed additive comprises 35-45 percent of alfalfa meal, 20-25 percent of folium cortex eucommiae powder, 20-25 percent of mulberry leaves powder, 4-9 percent of bone meal, 5-10 percent of saleratus and 0.5-1 percent of trace elements and composite vitamin additive, and the percent sum of raw materials is 100 percent. After the special feed additive is applied, the average ejaculate volume of breeder cocks is improved by 17.6 percent, the sperm activity is improved by 16.1 percent, and the sperm density is improved by 14.2 percent.

The invention relates to an artificial reproduction method for schizothorax wangchiachii. The method mainly comprises the following seven steps: bait preparation, parent fish sourcing, parent fish domestication, parent fish selection, artificial insemination, fish egg incubation and fish fry feeding. The artificial reproduction method for schizothorax wangchiachii has the beneficial effects that oxytocin is not used, the schizothorax wangchiachii can lay eggs and produce sperms, and relatively high fertility rate and hatchability can be obtained; the problem that female and male wild fish do not develop or develop asynchronously in a pond culture environment is solved fundamentally; the damage on the parent fish of the schizothorax wangchiachii is reduced, and the cultivation cost is also reduced; the method is reasonable and efficient, the hatchability is improved, and the waste of fish egg resources is reduced; and the aberration rate of the fish fry is reduced while the survival rate of the fish fry is improved.

The invention discloses a selective breeding method for improving meat performance of black goats of Guizhou. The selective breeding method includes the steps of: A, utilizing black goats of Guizhou as female parents, and utilizing adult black goats of Lezhi and adult goats of Boer as male parents; B, hybridizing the adult goats of Boer as the male parents with breeding ewes selected and remained in a generation F1 in an artificial insemination manner, and selecting and remaining breeding ewes from obtained lambs in generation F2 according to the same selecting and remaining method used for the generation F1; C, hybridizing other high-quality black goats of Lezhi as the male parents with the breeding ewes selected and remained in the generation F2 in an artificial insemination manner, and selecting and remaining breeding ewes from obtained lambs in generation F3 according to the same selecting and remaining method used for the generation F1; and D, subjecting breeding rams selected and remained in the generations F1-F3 and breeding ewes selected and remained in the generation F3 in an artificial insemination manner to intersect-breeding. By the selective breeding method, weight of six-month and one-year-old black goats of Guizhou is remarkably increased, and original dressing percentage of the one-year-old ewes is increased from 47.4% to 49.2%.

A method for the simultaneous determination of the total concentration of sperm cells and the proportion of live sperm cells in a semen sample is provided. By selective staining of live, dead and/or dying sperm cells, for example using one or more fluorochromes, the detection means can distinguish between live, dead and/or dying sperm cells, for example by detection of the fluorescent response from the sperm cells. The selective staining may be performed at room temperature and in concentrations below standard concentrations. The determination may be performed by selective counting of cells of each fluorescent quality for example by using a flow cytometer having internal concentration standard means. Furthermore, the likelihood of fertilizing a female animal with an insemination dose, taken from a semen sample having a known total concentration of sperm cells and a known proportion of live sperm cells, is predicted on the basis of the thus determined total concentration of sperm cells and the proportion of live sperm cells.

The invention discloses an antifreeze used for freezing and preserving boar semens as well as preparation and application thereof. The antifreeze used for freezing and preserving the boar semens, which is provided by the invention, contains pigeon egg yolk and concretely comprises the following components in parts by volume: 82 to 90 of freezing base liquid and 10 to 18 of pigeon egg yolk. The antifreeze used for freezing and preserving the boar semens, which is provided by the invention, has simple preparation method, easy material acquirement, reliable effect and easy popularization, is suitable for insemination after long-time preservation and long-distance transportation after the artificial insemination of the frozen semens of the tubules of boars and the ultra-low temperature freezing of the semens of the boars, can obviously enhance the survival rate of the semens, the integrity of the acrosomes and the integrity of the plasmalemma after the boar semens are frozen and unfrozen, and keeps the insemination activity of the boar semens.

The injector of the present invention (10) comprises a heat-retaining tube (12), an outer pipe (18) for inserting into a uterus which is fixed parallel to a front end of the heat-retaining tube (12), a syringe (22) for injection inserted into the heat-retaining tube (12) from the rear end of the heat-retaining tube (12), an elastic and flexible tube for injection (24) which is connected to the front end of the syringe (22) and is pulled out of the front end of the heat-retaining tube and is inserted slidably into inside of the hollow of the outer pipe (18),and a discharge nozzle (28) which is mounted integrally to the front end of the tube (24) and projects from the front end of the tube (18), and in which a portion between a projecting position from the heat-retaining tube (12) and the outer pipe (18) is regarded as a surplus length portion (24a) and the surplus length portion (24a) is paid out in a state that the outer pipe (18) is inserted to a given depth in a uterus, thereby making the discharge nozzle (28) reach to the deep portion of the uterus along the shape of the uterus.

A low-solinity artificial brooding and fresh-water culturing method for tawny puffer includes such steps as culturing parent fish, artificial insemination, artificial brooding, culturing minnow, and culturing in fresh water. Its advantages include unique method, simple operation, and high quality of tawny puffer.

The invention discloses a liquid for preserving bagged frozen pig semen and a method for preserving the same. The preservation liquid consists of liquid A and liquid B, wherein the liquid A contains 1 to 10 grams of glucose, 1 to 10 grams of lactose, 1 to 8 grams of sodium glutamate, 1 to 8 grams of polyvinylpyrrolidone, 100 milliliters of distilled water, 100 to 200 thousand of international units of antibiotic, 0.02 to 0.1 gram of vitamin B12, 0.02 to 0.1 gram of caffeine sodium benzoate, and 10 to 30 milliliters of egg yolk; and the liquid B contains 50 to 100 milliliters of the liquid A, 2 to 10 milliliters of glycerol, and 2 to 10 milliliters of N-N-dimethylformamide. The method for preserving the semen comprises the following steps: concentrating the semen after sampling the sperm, and diluting and refrigerating the liquid A with the concentrated semen proportionally and isothermally; diluting the mixture by using the liquid B for the second time, and subpackaging the semen into bags; and preserving the bagged semen in liquid nitrogen. The method is effective in maintaining the vitality of the sperms, has simple and convenient use and low cost, and can meet the needs of artificial insemination of the frozen pig semen.

The invention provides a manual schizothorax griseus pellegrin breeding method and belongs to the technical field of wild fish domestication and breeding, freshwater aquaculture and fish genetics-breeding. The manual schizothorax griseus pellegrin breeding method includes the following steps that 1, parent fish are caught and domesticated; 2, cultivation and spawning induction are conducted on the parent fish; 3, artificial insemination and egg hatching are conducted; 4, fry rearing is conducted. The manual schizothorax griseus pellegrin breeding method has the advantages that no report on the manual schizothorax griseus pellegrin breeding method exists in documents disclosed both home and abroad; the breeding method is scientific and reasonable, the rate of fertilization and the hatching rate of fries are higher and reach 98.6% and 91.2% respectively; the problem that no fry and fingerling exist for wild schizothorax griseus pellegrin resources is solved through manual recovery and supplement; the problem that no fry is supplied in the manual schizothorax griseus pellegrin breeding process is solved.

The invention discloses a method for establishing a full-female pelteobagrus fulvidraco breeding population, which comprises the following steps of: 1) manually hastening parturition and manually inseminating; 2) reversing sex; 3) identifying inheritance sex of the sex reversal pelteobagrus fulvidraco; 4) expanding the population; and 5) establishing the full-female pelteobagrus fulvidraco breeding population, so as to finally establish the full-female pelteobagrus fulvidraco breeding population. According to the invention, an integrating technology of hormone sex reversal combined with pelteobagrus fulvidraco chromosome peculiar molecular markers is adopted, so that XX physiological milter can be confirmed without adopting a test mating method and the breeding time is shortened; compared with the test mating method, one generation can be shortened; the gynogenesis step is omitted, so that the damage to the germ cell genetic material caused by manual induction gynogenesis is reduced; the yield of the XX raun is increased; and the economic and social benefits are obvious.

The invention relates to an artificial propagation method of Odontobutis obscura, belonging to the technical filed of artificial fish breeding, and comprising the following steps: parent fish selection; parent fish breeding; artificial insemination; germ cell delamination; artifical incubation; and fry breeding. The technical scheme provided by the invention solves the technical problem that the germ cell of the Odontobutis obscura is easy to mildew and rot, can ensure the fertility rate and rate of hatchability which are respectively over 66% and over 26%. The invention has simple operation, is not rigour, has wide applicability and can meet the requirement of industrialization breed in batch.