104 results about "Artificial insemination" patented technology

The invention relates to marine fish breeding, and particularly discloses an indoor full-artificial breeding method for black epinephelus, which specifically comprises the key technical links of nutrient enrichment of parent fish, hormone induced spawning, artificial insemination and fertilized egg incubation, indoor breeding of fry, compound feed domestication and the like. By controlling water temperature, illumination, salinity and nutritional conditions, synchronous maturation and efficient ovulation of parent gonads of the epinephelus nigatosus are achieved, the fertilization rate reaches 95% or above, and the hatching rate exceeds 85%. According to the method, the whole-process artificial controllable breeding of the epinephelus nigatosus from the parent fish to the fry is realized, a new variety is provided for the epinephelus culture industry, and a feasible technical approach is also provided for the artificial breeding and improved variety breeding of the wild epinephelus nigatosus in the island reef of the South China Sea.

The invention relates to an artificial cross breeding method for male epinephelus fuscoguttatus and female humpback perch, which belongs to the technical field of distant cross breeding and comprises the steps of parent selection of the epinephelus fuscoguttatus and the humpback perch, parent ripening cultivation, gamete collection, artificial insemination and incubation and fry cultivation. By means of reproductive regulation and distant hybridization technologies, two species of two genera of grouper family in different geographical distribution and different reproduction time are hybridized, namely cross-generic hybridization of grouper family breaks through the generic reproductive isolation, and therefore good varieties which grow fast and are high in survival rate are cultivated.

The utility model relates to a tool box for sheep artificial insemination, which comprises a box body and a lifting bag for containing the box body, the whole box body is of a square structure, an opening and closing bin for folding and opening is arranged on the box body, a corresponding opening and closing piece is arranged at a position on the lifting bag corresponding to the opening and closing bin, and the opening and closing piece is connected to a main body of the lifting bag through a zipper. The box body type structural design is adopted, the interior of the box body is divided into a plurality of parts, different tools are stored, meanwhile, the positions of the semen storage pipes are independently arranged, and the phenomenon that semen is polluted in the using process is prevented.

The invention discloses a cross breeding method for anti-ichthyophthirius mongholicus schizothorax prenanti. The method comprises the following steps: 1) selecting parents; (2) carrying out intensive cultivation on the parents; 3) synchronous artificial spawning induction; 4) artificial insemination; 5) hatching management; (6) breeding the schizothorax prenanti (female) and percocypris pingi (sex) hybrid fry; and (7) measuring the ichthyophthirius multifiliis resistance and high-temperature tolerance of the schizothorax prenanti (female) * percocypris pingi (sex) hybrid fry. The ichthyophthirius ichthyophthirius infection rate of the bred schizothorax prenanti (female) and percocypris pingi (sex) hybrid offspring is reduced by 31.95% compared with that of the schizothorax prenanti (female) and percocypris pingi (sex) selfing offspring; the death rate is only 0.33% and is reduced by 73% compared with that of the selfing filial generation of the schizothorax prenanti; the high-temperature tolerance range of the schizothorax prenanti selfing offspring is improved by 4-5 DEG C compared with that of the schizothorax Therefore, the method disclosed by the invention can be used for successfully culturing a hybrid variety which is high in ichthyophthirius multifiliis resistance, relatively high in economic value and relatively high in high-temperature tolerance.

The utility model particularly relates to an acrossocheilus fasciatus artificial induction spawning inspection fish nest, and belongs to the technical field of fish culture, the fish nest comprises a box body, a microflow assembly and a gravel layer, the box body is provided with a containing cavity, and the box body comprises at least one inlet and outlet which is formed in the side surface of the box body; the microflow assembly is connected with the box body, the microflow assembly is provided with a microflow path at least partially extending and penetrating through the microflow assembly, and the microflow path is communicated with the external space and a water area of the containing cavity and used for forming micro water flow; the gravel layer is arranged in the containing cavity. The spawning site can be provided for acrossocheilus fasciatus, acrossocheilus fasciatus in the oestrus period is artificially induced to enter the spawning site to prepare spawning, acrossocheilus fasciatus with mature gonad can be caught for inspection, artificial insemination work can be carried out timely, operation is convenient, and the manual labor intensity can be reduced.

The utility model relates to the field of artificial insemination, in particular to a semen collection device, which comprises a semen collection device body which comprises a semen collection device, and is characterized in that the body further comprises a holding part connected with the semen collection device, the holding part comprises a telescopic component with two ends extending out of the body, and the telescopic component is provided with an opening. The grips are arranged at the two ends of the telescopic component; according to the semen collection device, the holding part is additionally arranged on an original semen collection device, the holding part is formed by combining the telescopic component and the holding handle, in the actual operation process, the holding handle provides the corresponding holding effect, instability in the collection process is reduced, and the operation difficulty is lowered; the telescopic component is convenient to store, due to the fact that the grip is used, the transverse occupied space of the whole semen collection device can be increased, under the action of the telescopic component, the grip retracts into the body, and therefore the problem that storage is inconvenient is solved.

This invention discloses a method for efficiently inducing allopolyploid fish fry, belonging to the field of fish breeding technology. The method of this invention includes the following technical steps: (1) Induction of spawning in distant hybridization parents: Selecting female and male parent fish from different subfamilies of Cyprinidae, and collecting mature eggs and semen respectively; (2) Obtaining hybrid fertilized eggs: Mixing the collected eggs with semen, and performing dry artificial insemination to obtain hybrid fertilized eggs; (3) Inducing allopolyploidy: Placing the fertilized eggs in a water bath for heat shock treatment to inhibit the expulsion of the second polar body or inhibit the first cleavage, and then quickly transferring the treated fertilized eggs to aquaculture water to continue hatching fry. The method of this invention combines the method of distant hybridization between subfamilies with the method of heat shock induction. After induction using this method, the multiplication rate of hatched fry can reach 100%, which significantly improves the yield of allopolyploid fish fry from distant hybridization.

The utility model provides an animal sperm injector convenient to use, and relates to the technical field of animal artificial insemination. The animal semen injection device convenient to use comprises a semen injection supporting rod body, a semen injection elastic outer sleeve, an easily-folded leakage-proof piece and a blocking piece. The sperm injection elastic outer sleeve is arranged on the sperm injection supporting rod body in a sleeving mode, the two ends of the sperm injection elastic outer sleeve are connected with the sperm injection supporting rod body in a sealed mode, and a containing cavity used for containing sperm is formed between the sperm injection supporting rod body and the sperm injection elastic outer sleeve; the front end of the sperm injection supporting rod body is provided with a first mounting hole for mounting the easy-to-break leakage-proof piece, and the first mounting hole is communicated with the accommodating cavity; a second mounting hole for mounting the blocking piece is formed in the tail end of the semen injection supporting rod body, and the second mounting hole is communicated with the containing cavity; and temperature control wax is arranged in the easy-to-fold leakage-proof piece. The technical effect that the fertilization device is convenient to use is achieved.

The invention belongs to the technical field of biology, and particularly relates to application of N-Acetyl-D-Glucosamine as an additive in a boar semen diluent preserved at 17 DEG C. The invention further relates to a preparation method of the N-Acetyl-D-Glucosamine. By adding 2.5 mM of N-Acetyl-D-Glucosamine into the boar semen diluent, the activity and the forward movement proportion of boar sperms can be remarkably improved under the preservation condition of 17 DEG C, and meanwhile, no adverse effect is caused on the lipid oxidation state and acrosome integrity of the boar sperms. The method is easy and convenient to operate, low in cost, suitable for the artificial insemination process of a large-scale pig farm and capable of effectively prolonging the preservation time of the boar semen and improving the breeding efficiency.

The utility model relates to the technical field of livestock and poultry artificial insemination, and discloses a distribution structure and a semen deposition gun, the distribution structure adopts a pump structure and is provided with a pump body and a piston rod, the piston rod is matched in a pump cavity of the pump body to realize automatic suction or pumping of semen with a preset volume, manual suction by an operator is not needed, and the labor intensity of the operator is reduced. Automatic semen transfer in the artificial insemination process is achieved, and the semen transfer efficiency is effectively improved.

The invention relates to the technical field of poultry breed conservation biology, and discloses a cryopreservation kit for chicken semen and a use method of the cryopreservation kit. The invention relates to a cryopreservation kit for chicken semen. The cryopreservation kit comprises a cryopreservation diluent, a cryoprotectant, an insemination diluent, a DMEM (dulbecco's modified eagle medium) and a frozen semen tube. The cryopreservation diluent in the kit provided by the invention can provide a better living environment for chicken sperms, and the cryoprotectant in the kit can successfully perform cryopreservation on the chicken sperms; when the kit provided by the invention is used for freezing and storing chicken semen, the unfrozen chicken semen is used for artificial insemination, the fertilization rate can reach 70% or above, and the requirement for breed conservation of chicken genetic resources is met.

The utility model provides an artificial insemination table for breeding cattle and sheep, and relates to the field of artificial insemination. The artificial insemination table for cattle and sheep breeding comprises a fixing base and a supporting table, a connecting frame is fixedly connected to the bottom face of the supporting table, a body limiting mechanism for fixing the body is arranged on the surface of the fixing base and comprises a supporting frame and a binding belt, a fixing rod is hinged to the surface of the supporting frame, and the binding belt is arranged on the supporting frame. Limb fixing mechanisms for fixing four limbs are arranged at the two ends of the connecting frame. According to the artificial insemination table for cattle and sheep breeding, the livestock body is supported through the supporting table, the livestock body is fixed to the surface of the supporting table through the binding belt, the neck of the livestock is supported and fixed through the supporting frame and the fixing rod, the phenomenon that the head of the livestock swings at will during an operation is avoided, and the connecting sleeve is fixed through the connecting frame; and then the connecting blocks drive the fixing belts to move to fix the four limbs of the livestock, the four limbs of the livestock are prevented from swinging, and the artificial insemination efficiency is improved.

Artificial cervical fluid is disclosed that contains a mucilaginous extract from the okra plant. The mucilaginous extract can be produced using a hot aqueous extractant or cold extraction process followed by separation of larger particles from the extract. The extract finds many uses, for example as a sperm storage medium, a sperm freezing medium, a sexual lubricant, an artificial insemination medium, and an in vitro fertilization medium.

The invention provides an artificial propagation method of schizothorax wangchizoides, and belongs to the technical field of fish propagation. The artificial propagation method of the schizothorax wandersoni comprises the following steps that (1) mature parent fish is selected and injected with an oxytocic mixture, and the oxytocic mixture comprises DOM, HCG and LRH-A3; (2) collecting ova and sperms after the parent fish is oestrus, and carrying out artificial insemination; and (3) transferring the fertilized eggs into an incubator for incubation. According to the method, 268345 artificial fertilized eggs are obtained, the fertilization rate reaches 95.6%, 251707 pond fries are obtained after artificial incubation, the hatching rate is 93.8%, and the fertilization rate and the hatching rate of the schizothorax wandersoni are remarkably increased.

Methods, uses and compositions including FSH for use in the treatment of infertility by intrauterine insemination are described.

The invention provides a method for efficiently inducing larimichthys crocea triploid by using hydrostatic pressure, which comprises the following steps of: immediately transferring fertilized eggs into a hydrostatic pressure device 2 minutes and 30 seconds after artificial insemination of larimichthys crocea sperm eggs, applying pressure of 7000-8000 PSI, and performing hydrostatic pressure treatment for 3 minutes. Wherein in the hydrostatic pressure treatment process, it should be ensured that the pressure is stable, and the fluctuation range does not exceed + / -1%. According to the method, the time and pressure of hydrostatic pressure treatment are accurately controlled, the triploid induction rate can be 90% or above, and the induction rate is high. The damage to fertilized eggs is reduced through optimized processing parameters, the hatching rate of the fries can reach 70% or above, and the hatching rate is high. In addition, the hydrostatic pressure treatment time is only 3 minutes, the operation is efficient, the method is more suitable for large-scale production compared with other methods, and the triploid cultivation efficiency is improved.

The application provides a dairy cow mating auxiliary device, which comprises two symmetrically distributed side frames, a front frame capable of clamping a neck of a cow is fixed between one end of the two side frames, the other end of one of the side frames is provided with a clamping ring with a circular ring structure, the ring wall of the clamping ring is a hollow structure, the inner ring wall of the clamping ring is made of elastic rubber material, and the ring wall of the clamping ring is connected with a gas pressure adjusting device capable of adjusting the gas pressure in the ring wall. In the artificial insemination process, the labor intensity of the staff can be reduced without artificial hand pulling of the cow tail, and the stability of the cow tail clamped by the inner ring wall is relatively high, so that the cow tail cannot break away from the clamping ring even if the cow resists and swings the tail.

The invention discloses an out-of-season artificial propagation technical method for crucian carp. The technical method specifically comprises the key steps of water temperature and illumination control of an intelligent culture system, parent fish screening and concentrated intensified culture, parent fish ripening acceleration, artificial spawning induction, artificial insemination, incubation and the like. Aiming at seasonal limitation of natural breeding of the crucian carp, by scientifically regulating and controlling an illumination period and a water temperature environment and accurately simulating ecological conditions suitable for breeding of the crucian carp, it is ensured that parent fishes of the crucian carp can synchronously reach physiological states of periodic normal ovulation and spermiation in an unnatural spawning season from October to January of the next year; the activity and quality of germ cells can be obviously improved; meanwhile, an innovative breeding mode and an exclusive feeding scheme are combined, the industrial pain points that eggs of female crucian carp of ice cream are prone to aging and parent fishes only spawn once a year in a natural state are effectively overcome, and anti-season stable breeding is achieved.

The invention relates to the technical field of poultry breeding, and discloses a disinfection structure and an artificial insemination gun, an ultraviolet light source is adopted to emit ultraviolet light to irradiate the periphery of an insemination pin, so that the surface of the insemination pin is disinfected after each insemination, the insemination pin is prevented from being polluted, cross infection is avoided, and the artificial insemination gun is safe and reliable. Therefore, the health of the poultry during the continuous insemination operation can be guaranteed.

The utility model relates to a sheep artificial insemination cage which comprises a cage body and a clamping assembly arranged in the cage body and used for fixing sheep, the cage body is of a transverse cuboid frame-shaped structure, an opening and closing door is arranged on the rear portion of the cage body, a fence and a feeding trough are arranged on the front portion of the cage body, and partition plates are arranged on the two sides of the cage body respectively. The clamping assembly comprises a sheep neck clamp arranged on a fence on the front portion of the cage body. An independent cage body structure is adopted, a corresponding mechanism for fixing sheep is arranged in the cage, the sheep can be effectively fixed in the actual insemination process, insemination operation is facilitated, and contact infection between the sheep can be effectively avoided through the independent cage body.

This invention discloses an artificial breeding method for the Oriental blackfish, belonging to the field of fish artificial breeding technology. It includes six core steps: broodstock selection and domestication, enhanced maturation cultivation, broodstock selection and anesthesia, spawning induction, artificial insemination, fertilized egg hatching, and fry rearing. One year in advance, suitable-aged wild Oriental blackfish are selected and domesticated in a flowing water pond. Before breeding, tocopherol is mixed into their feed to enhance gonadal development. High-quality broodstock are selected at a female-to-male ratio of 1:2 and anesthetized. A specific ratio of spawning inducing agent is injected using a two-injection method. Artificial insemination is performed in a standardized fiberglass circular fishpond under controlled water flow conditions. Fertilized eggs are incubated at 26±1℃ for 22 hours, and after hatching, they are transferred to a sterilized micro-flow water pond for rearing. This invention achieves a 100% spawning efficiency, a stable fertilization rate of over 95%, and a hatching rate exceeding 82%. It is simple to operate, cost-effective, and allows for large-scale breeding, providing key technical support for the conservation and population expansion of the Oriental blackfish.

The invention relates to a three-way hybrid grouper new germplasm cultivation method, and belongs to the technical field of grouper genetic breeding. According to the method, epinephelus moara and golden tiger hybridized fries (female epinephelus fuscoguttatus * male epinephelus coioides) are used as breeding materials, three-way hybridization is carried out in a matching mode of female epinephelus moara * male epinephelus coioides, and breeding of three epinephelus genetic species and fry with excellent characteristics is achieved. The method comprises the steps of parent selection, parent fish reproduction regulation and control, parent fish spawning induction, gamete collection, artificial insemination, incubation and fry rearing. According to the method, on the basis of reproductive regulation and crossbreeding technologies, time and space reproductive isolation of the groupers is broken through, and the new grouper germplasm which is high in survival rate, low in malformation rate and high in growth speed is cultivated.

The invention relates to a low-temperature preservation method for sheep semen, and belongs to the technical field of animal reproduction. The invention provides a method for low-temperature preservation of sheep semen, which comprises the following steps: (1) collecting breeding male sheep semen by a false vagina method to obtain the collected sheep semen; (2) mixing the sheep semen collected in the step (1) with a semen diluent and fullerene to obtain a sheep semen diluent; and (3) preserving the sheep semen diluent under the condition of 2-8 DEG C. The sheep semen is preserved according to the method, the effective application time of sperm preservation can be remarkably prolonged, the blastocyst forming rate of in-vitro fertilization embryos can be remarkably increased, and the method is applied to reproduction technologies such as artificial insemination (AI) and in-vitro fertilization (IVF) of sheep, the utilization rate of high-quality breeding rams can be increased, and genetic resource protection and efficient breeding can be promoted.

The invention relates to a method for increasing the lifespan of animal sperm comprising contacting said sperm with an inhibitor of Slo3 potassium channel.The invention also relates to a use of an inhibitor of Slo3 potassium channel, for increasing the lifespan of animal sperm or motility of capacitated animal sperm, comprising contacting an inhibitor of Slo3 potassium channel with said sperm. Moreover, the invention relates to an artificial insemination instrument for use in artificial insemination of an animal, comprising animal sperm in contact with an inhibitor of Slo3 potassium channel.The invention also relates to a method for artificially inseminating an animal using said artificial insemination instrument.Eventually, the invention relates to a method for increasing the fertility of an animal, comprising contacting sperm of said animal with an inhibitor of Slo3 potassium channel; then artificially inseminating said animal with said sperm.

The invention provides a method for increasing the hatching rate of fertilized eggs of Epinephelus yunnanensis, and belongs to the technical field of fish culture. The method comprises the following steps: S1, parent cultivation and male transformation: selecting five-year-old Epinephelus lanceolatus without seminal fluid as a male parent to perform indoor circulating water cultivation, and inducing male transformation in a cooperative mode of feeding a feed containing methyltestosterone, injecting follicle-stimulating hormone and periodically increasing and decreasing water temperature in the cultivation process; s2, male fish screening: regularly checking the male conversion rate in the parents in the step S1, screening the male fishes with qualified maturity, and screening the male fishes with qualified sperm maturity from the male fishes with qualified maturity; s3, insemination and incubation: collecting the seminal fluid of the male fishes screened in the step S2 for artificial insemination of the eggs of the epinephelus moara, and incubating the fertilized eggs. According to the method, the problems of small male fish quantity, insufficient seminal fluid and low fertilized egg hatching rate in artificial breeding of groupers are solved.

The invention is suitable for the technical field of artificial insemination equipment, and provides accurate positioning insemination equipment for artificial insemination of mutton sheep. A mounting groove is formed in the flow guide sleeve, the mounting groove extends and penetrates in the length direction of the flow guide sleeve, and an injector for storing semen is arranged in the mounting groove; the injector comprises an injector body and an injection rod, and the injection rod is inserted into the injector body; an extending pipe is arranged at the output end of the injector; the rotating seat is rotationally arranged at the end part of the flow guide sleeve; the first micro camera is arranged at one end, away from the rotating seat, of the guide sleeve; the second micro camera is arranged at the output end of the extending pipe; the driving mechanism is arranged between the rotating seat and the injector and is used for driving the injector to move or inject; and a display device. The first micro camera, the second micro camera and the display device are arranged to be matched so that semen can be accurately injected to a proper position, meanwhile, the grab handle is arranged, operation can be achieved with one hand, operation is easy, and use is convenient.

The invention relates to an artificial insemination device (1) for livestock, particularly pigs, comprising a tubular body (2) and a tip (3) attached at one end (6) of the tubular body (2) and configured to be inserted and positioned in the cervix of a female animal, characterized in that the tip (3) is composed of at least agar-agar, water, and glycerol. Figure for the abstract: 3

The application belongs to the technical field of molecular biology, and particularly relates to a method for improving meat quality of mutton sheep by using a SNP site of a FASN gene. The method for improving meat quality of mutton sheep by using the SNP site of the FASN gene comprises the following steps: detecting whether a g.50277076C>T site mutation exists in a FASN gene in a genome of a mutton sheep to be detected; selecting a mutton sheep individual with a genotype of TT type to perform artificial insemination or intrabreed. The application uses the above-mentioned molecular marker as a reliable marker of meat quality of Mongolian sheep, establishes a technical system for DNA detection of the genotype, improves selection intensity, and accuracy and efficiency of breeding, and improves meat quality of the Mongolian sheep.

The invention relates to a method for applying an AK4 gene molecular marker to duck high sperm curve rate early breeding, and belongs to the technical field of biology, SNP genetic typing is carried out by utilizing a sequencing technology, the AK4 gene molecular marker obviously related to sperm curve rate is obtained through whole genome association analysis and screening, and in breeding, according to a breeding target, the AK4 gene molecular marker is applied to duck high sperm curve rate early breeding. By eliminating G / A genotype and A / A genotype individuals and retaining G / G genotype individuals, the reproductive performance of the breeding ducks is improved, the artificial insemination efficiency is optimized, and the breeding progress is accelerated.

The present application belongs to the field of fish breeding, and discloses a method for simultaneously breeding natural gynogenesis of Procyprinus and hybrid offspring of Cyprinus carpio, comprising the following steps: taking Procyprinus as the female parent and taking red crucian carp as the male parent to perform special pond breeding, after artificial induction of parturition, performing artificial insemination after the parent fish is in estrus, then performing flow water hatching on the fertilized eggs, after the fry is hatched, performing feeding, and then performing detection and screening on the fed fish, thereby obtaining the natural gynogenesis of Procyprinus and the hybrid offspring of Cyprinus carpio. The present application changes the activity of the ovum and sperm of the parent fish by performing distant hybridization of Procyprinus and red crucian carp and combining with specific types and dosages of parturition inducers, and thereby obtains the natural gynogenesis of Procyprinus and the hybrid offspring of Cyprinus carpio which are both sexually fertile.