186 results about "Artificial insemination" patented technology

The invention provides a method for cultivating hybrid fries of Jinhu and tiger. The method comprises the following steps: selecting a breeding environment; breeding and ripening female parents; management and control before oviposition; insemination; hatching fertilized eggs; fry rearing. According to the method, in the female parent breeding and ripening stage, specific biological bait is controlled to be fed in stages, feeding of the parent fish can be promoted, growth can be accelerated, in cooperation with specific compound feed in the management and control stage before oviposition, favorable conditions are created for subsequent oviposition, and the quality and number of ova can be improved. Low-frequency ultrasonic assisted fertilization is introduced in the artificial insemination stage, so that the activity of ovum sperms can be obviously improved, and the fertilization rate is increased; in the fertilized egg hatching stage, intermittent ultrasound is assisted, the hatching rate can be increased, and the seedling yield of the golden-tiger hybrid spots can be increased; after hatching, transferring into a specific culture pond, slowly releasing beneficial bacteria, improving the adaptability of the fry, adsorbing harmful substances, reducing pathogenic bacteria, stabilizing water quality, promoting the growth of the fry, improving the survival rate and reducing the malformation rate.

The invention relates to marine fish breeding, and particularly discloses an indoor full-artificial breeding method for black epinephelus, which specifically comprises the key technical links of nutrient enrichment of parent fish, hormone induced spawning, artificial insemination and fertilized egg incubation, indoor breeding of fry, compound feed domestication and the like. By controlling water temperature, illumination, salinity and nutritional conditions, synchronous maturation and efficient ovulation of parent gonads of the epinephelus nigatosus are achieved, the fertilization rate reaches 95% or above, and the hatching rate exceeds 85%. According to the method, the whole-process artificial controllable breeding of the epinephelus nigatosus from the parent fish to the fry is realized, a new variety is provided for the epinephelus culture industry, and a feasible technical approach is also provided for the artificial breeding and improved variety breeding of the wild epinephelus nigatosus in the island reef of the South China Sea.

The invention relates to an artificial cross breeding method for male epinephelus fuscoguttatus and female humpback perch, which belongs to the technical field of distant cross breeding and comprises the steps of parent selection of the epinephelus fuscoguttatus and the humpback perch, parent ripening cultivation, gamete collection, artificial insemination and incubation and fry cultivation. By means of reproductive regulation and distant hybridization technologies, two species of two genera of grouper family in different geographical distribution and different reproduction time are hybridized, namely cross-generic hybridization of grouper family breaks through the generic reproductive isolation, and therefore good varieties which grow fast and are high in survival rate are cultivated.

The invention provides an excrement weight change and panda oestrus cycle correlation modeling method and system, and relates to the technical field of panda breeding, and the method comprises the steps: obtaining basic monitoring data of female panda individuals; performing fiber metabolism characteristic extraction according to the daily excrement weighing value to obtain a bamboo fiber metabolism efficiency sequence; performing matrix construction according to the metabolic efficiency sequence and the serum progesterone concentration detection value to obtain a progesterone-metabolic coupling matrix; correcting according to the natural season classification label to obtain an inhibition factor; identifying according to the progesterone-metabolism coupling matrix and the inhibition factor to obtain an estrus critical state characteristic value; and performing model construction according to the metabolic efficiency sequence, the progesterone-metabolic coupling matrix, the inhibition factor and the estrus critical state characteristic value to obtain a panda estrus window dynamic prediction model. According to the method, the missing rate of artificial insemination window identification is reduced, and the breeding efficiency of endangered species is greatly improved.

According to the Hainan pig semen preservation diluent as well as the preparation method, the application and the detection method thereof disclosed by the invention, galangin and kaempferide are added into a basic diluent to develop a diluent formula capable of efficiently preserving semen at a low temperature (4 DEG C); the galangin and the kaempferol added into the diluent have a good sperm protection effect and a certain antibacterial ability and can replace antibiotics, and bovine serum albumin (BSA) is further combined as a low-temperature protective agent, so that sperm movement parameters are improved, and the low-temperature preservation time of the boar semen is effectively prolonged; the use amount of antibiotics in pig raising production is reduced, the problem of drug resistance of bacteria can be effectively relieved, and the use of the antibiotics and the generation of drug-resistant bacteria are greatly reduced. According to the method, the semen utilization rate of excellent breeding boars is increased, sow health is guaranteed, the conception rate of deep insemination is increased, and efficient and low-cost artificial insemination is achieved.

The invention discloses a Tibetan ink head fish artificial propagation method, and belongs to the field of aquaculture. The Tibetan ink head fish artificial propagation method comprises the following steps that male fish parents and female fish parents which are sexually mature are selected, and spawning induction substances are injected into the female fish twice; the male fish injects spawning induction substances at one time when the female fish is injected for the second time; carrying out in-vitro artificial insemination 12 hours after injecting the oxytocic substance to obtain fertilized eggs; hatching the fertilized eggs, increasing the concentration of lysozyme in a hatching system after the membranes of the fish fries are broken in the hatching process, and artificially assisting the membranes to obtain the Tibetan black head fish fries; and feeding the Tibetan ink head fish fries in stages. According to the artificial propagation method for the ink head fish in Tibetan, the fry of the ink head fish in Tibetan is successfully obtained, the quality and the survival rate of the fry are guaranteed, and technical support is provided for artificial propagation of the fry.

The utility model relates to a tool box for sheep artificial insemination, which comprises a box body and a lifting bag for containing the box body, the whole box body is of a square structure, an opening and closing bin for folding and opening is arranged on the box body, a corresponding opening and closing piece is arranged at a position on the lifting bag corresponding to the opening and closing bin, and the opening and closing piece is connected to a main body of the lifting bag through a zipper. The box body type structural design is adopted, the interior of the box body is divided into a plurality of parts, different tools are stored, meanwhile, the positions of the semen storage pipes are independently arranged, and the phenomenon that semen is polluted in the using process is prevented.

The invention discloses a cross breeding method for anti-ichthyophthirius mongholicus schizothorax prenanti. The method comprises the following steps: 1) selecting parents; (2) carrying out intensive cultivation on the parents; 3) synchronous artificial spawning induction; 4) artificial insemination; 5) hatching management; (6) breeding the schizothorax prenanti (female) and percocypris pingi (sex) hybrid fry; and (7) measuring the ichthyophthirius multifiliis resistance and high-temperature tolerance of the schizothorax prenanti (female) * percocypris pingi (sex) hybrid fry. The ichthyophthirius ichthyophthirius infection rate of the bred schizothorax prenanti (female) and percocypris pingi (sex) hybrid offspring is reduced by 31.95% compared with that of the schizothorax prenanti (female) and percocypris pingi (sex) selfing offspring; the death rate is only 0.33% and is reduced by 73% compared with that of the selfing filial generation of the schizothorax prenanti; the high-temperature tolerance range of the schizothorax prenanti selfing offspring is improved by 4-5 DEG C compared with that of the schizothorax Therefore, the method disclosed by the invention can be used for successfully culturing a hybrid variety which is high in ichthyophthirius multifiliis resistance, relatively high in economic value and relatively high in high-temperature tolerance.

Artificial cervical fluid is disclosed that contains a mucilaginous extract from the okra plant. The mucilaginous extract can be produced using a hot aqueous extractant or cold extraction process followed by separation of larger particles from the extract. The extract finds many uses, for example as a sperm storage medium, a sperm freezing medium, a sexual lubricant, an artificial insemination medium, an in vitro fertilization medium, and methods for treating and / or preventing infection, replication, and transmission of viruses, such as sexually transmitted viruses.

The utility model particularly relates to an acrossocheilus fasciatus artificial induction spawning inspection fish nest, and belongs to the technical field of fish culture, the fish nest comprises a box body, a microflow assembly and a gravel layer, the box body is provided with a containing cavity, and the box body comprises at least one inlet and outlet which is formed in the side surface of the box body; the microflow assembly is connected with the box body, the microflow assembly is provided with a microflow path at least partially extending and penetrating through the microflow assembly, and the microflow path is communicated with the external space and a water area of the containing cavity and used for forming micro water flow; the gravel layer is arranged in the containing cavity. The spawning site can be provided for acrossocheilus fasciatus, acrossocheilus fasciatus in the oestrus period is artificially induced to enter the spawning site to prepare spawning, acrossocheilus fasciatus with mature gonad can be caught for inspection, artificial insemination work can be carried out timely, operation is convenient, and the manual labor intensity can be reduced.

The invention relates to the technical field of cock breeding, in particular to a biomarker, a preparation method and application thereof and a method for assisting in breeding cock semen quality identification. The biomarker comprises the following components: phosphatidylcholine, lysophosphatidylcholine, phosphatidyl ethanolamine, lysophosphatidyl ethanolamine, phosphatidylserine and lysophosphatidylserine. According to the invention, key markers can be rapidly detected from the semen so as to determine breeding suitable for liquid-state preservation of the semen and high-activity individuals. The artificial insemination interval time can be greatly prolonged, and long-time high-sperm active breeding cock individuals can be rapidly bred. The breeder cock breeding amount can be reduced, grains are saved, and breeding benefits are greatly improved.

The invention discloses an epigenetics method for improving the cryopreservation efficiency of sheep semen. The method comprises the following steps: extracting seminal fluid of Donflilien and Hu sheep hybrid F1-generation sheep, dividing the seminal fluid into a fresh group and a frozen group, carrying out somatic cell removal and small non-coding RNA extraction, screening out differentially expressed microRNAs by utilizing a Pandorah sequencing technology, analyzing a target gene and a signal channel of the microRNAs, and determining the seminal fluid of the Donflilien and Hu sheep hybrid F1-generation sheep. The small non-coding RNA related to sperm cryopreservation is found to mainly relate to key biological processes such as oxidative stress response and cell surface receptor signal channels. The screened differentially expressed small non-coding RNA is added into the frozen semen through methods such as in vitro chemical synthesis, so that the artificial fertilization conception rate of the frozen semen is remarkably increased.

The invention relates to the technical field of artificial cultivation of oysters, in particular to a method for artificially cultivating oysters. Comprising the following steps: (1) selecting oyster parents, and after ripening, carrying out artificial insemination and hatching to obtain oyster larvae; a ripening agent is used during ripening; (2) cultivating the oyster larvae obtained in the step (1), feeding the oyster larvae with initial feed to obtain young oysters, and feeding the young oysters with artificial feed after the young oysters are attached and metamorphosed; (3) performing intermediate culture on the oyster seedlings obtained in the step (2) to obtain juvenile mollusks; and (4) transferring the juvenile mollusks to a breeding farm to breed for 12-18 months. According to the method, high yield, high efficiency and high quality of oyster culture are achieved through an innovative ripening technology, precise nutrition allocation and scientific environment management, a reliable solution is provided for sustainable development of the oyster industry, and the method has wide application prospects.

The application provides a method for on-site rapid artificial insemination of wild Pseudosciaena crocea of Lutjanidae, and belongs to the technical field of aquatic animal breeding. Wild parent fish of Pseudosciaena crocea of Lutjanidae are captured in the breeding season; sperm is collected from the selected sexually mature male parent fish on the capture site; after artificial sperm collection, sexually mature female parent fish are selected, and fish eggs are collected; high-quality fertilized eggs are obtained through artificial insemination; the artificial insemination comprises the following steps: adding sperm into fish eggs, adding natural seawater for stirring, and finally washing with natural seawater. Through comprehensive regulation of key links in the whole process of artificial insemination, the purpose of obtaining high-quality fertilized eggs by using wild parent fish of Pseudosciaena crocea of Lutjanidae and carrying out artificial fry breeding is achieved.

The utility model relates to the field of artificial insemination, in particular to a semen collection device, which comprises a semen collection device body which comprises a semen collection device, and is characterized in that the body further comprises a holding part connected with the semen collection device, the holding part comprises a telescopic component with two ends extending out of the body, and the telescopic component is provided with an opening. The grips are arranged at the two ends of the telescopic component; according to the semen collection device, the holding part is additionally arranged on an original semen collection device, the holding part is formed by combining the telescopic component and the holding handle, in the actual operation process, the holding handle provides the corresponding holding effect, instability in the collection process is reduced, and the operation difficulty is lowered; the telescopic component is convenient to store, due to the fact that the grip is used, the transverse occupied space of the whole semen collection device can be increased, under the action of the telescopic component, the grip retracts into the body, and therefore the problem that storage is inconvenient is solved.

This invention discloses a method for efficiently inducing allopolyploid fish fry, belonging to the field of fish breeding technology. The method of this invention includes the following technical steps: (1) Induction of spawning in distant hybridization parents: Selecting female and male parent fish from different subfamilies of Cyprinidae, and collecting mature eggs and semen respectively; (2) Obtaining hybrid fertilized eggs: Mixing the collected eggs with semen, and performing dry artificial insemination to obtain hybrid fertilized eggs; (3) Inducing allopolyploidy: Placing the fertilized eggs in a water bath for heat shock treatment to inhibit the expulsion of the second polar body or inhibit the first cleavage, and then quickly transferring the treated fertilized eggs to aquaculture water to continue hatching fry. The method of this invention combines the method of distant hybridization between subfamilies with the method of heat shock induction. After induction using this method, the multiplication rate of hatched fry can reach 100%, which significantly improves the yield of allopolyploid fish fry from distant hybridization.

The utility model provides an animal sperm injector convenient to use, and relates to the technical field of animal artificial insemination. The animal semen injection device convenient to use comprises a semen injection supporting rod body, a semen injection elastic outer sleeve, an easily-folded leakage-proof piece and a blocking piece. The sperm injection elastic outer sleeve is arranged on the sperm injection supporting rod body in a sleeving mode, the two ends of the sperm injection elastic outer sleeve are connected with the sperm injection supporting rod body in a sealed mode, and a containing cavity used for containing sperm is formed between the sperm injection supporting rod body and the sperm injection elastic outer sleeve; the front end of the sperm injection supporting rod body is provided with a first mounting hole for mounting the easy-to-break leakage-proof piece, and the first mounting hole is communicated with the accommodating cavity; a second mounting hole for mounting the blocking piece is formed in the tail end of the semen injection supporting rod body, and the second mounting hole is communicated with the containing cavity; and temperature control wax is arranged in the easy-to-fold leakage-proof piece. The technical effect that the fertilization device is convenient to use is achieved.

The invention belongs to the technical field of biology, and particularly relates to application of N-Acetyl-D-Glucosamine as an additive in a boar semen diluent preserved at 17 DEG C. The invention further relates to a preparation method of the N-Acetyl-D-Glucosamine. By adding 2.5 mM of N-Acetyl-D-Glucosamine into the boar semen diluent, the activity and the forward movement proportion of boar sperms can be remarkably improved under the preservation condition of 17 DEG C, and meanwhile, no adverse effect is caused on the lipid oxidation state and acrosome integrity of the boar sperms. The method is easy and convenient to operate, low in cost, suitable for the artificial insemination process of a large-scale pig farm and capable of effectively prolonging the preservation time of the boar semen and improving the breeding efficiency.

The utility model relates to the technical field of livestock and poultry artificial insemination, and discloses a distribution structure and a semen deposition gun, the distribution structure adopts a pump structure and is provided with a pump body and a piston rod, the piston rod is matched in a pump cavity of the pump body to realize automatic suction or pumping of semen with a preset volume, manual suction by an operator is not needed, and the labor intensity of the operator is reduced. Automatic semen transfer in the artificial insemination process is achieved, and the semen transfer efficiency is effectively improved.

The invention relates to the technical field of poultry breed conservation biology, and discloses a cryopreservation kit for chicken semen and a use method of the cryopreservation kit. The invention relates to a cryopreservation kit for chicken semen. The cryopreservation kit comprises a cryopreservation diluent, a cryoprotectant, an insemination diluent, a DMEM (dulbecco's modified eagle medium) and a frozen semen tube. The cryopreservation diluent in the kit provided by the invention can provide a better living environment for chicken sperms, and the cryoprotectant in the kit can successfully perform cryopreservation on the chicken sperms; when the kit provided by the invention is used for freezing and storing chicken semen, the unfrozen chicken semen is used for artificial insemination, the fertilization rate can reach 70% or above, and the requirement for breed conservation of chicken genetic resources is met.

The invention provides a seminal fluid freezing and transferring device for livestock insemination, and belongs to the technical field of livestock artificial insemination, the seminal fluid freezing and transferring device comprises an outer box body, a freezing bin and an unfreezing bin, the freezing bin and the unfreezing bin are arranged in the outer box body, and the freezing bin and the unfreezing bin are two independent spaces in the outer box body respectively; the freezing bin is used for storing frozen semen; the unfreezing bin is used for unfreezing the frozen semen; the freezing bin comprises a freezing container capable of being closed and a thin tube support arranged in the freezing container. A plurality of frozen fine tubes can be placed on the fine tube support, and the frozen fine tubes placed on the fine tube support are arranged in the height direction of the outer box body. The unfreezing bin comprises a heating device, a freezing bin and the heating device, and the heating device is used for heating the frozen semen tubule in a frozen state to restore the frozen semen tubule to a normal temperature state in which artificial insemination can be carried out; by means of the semen freezing and transferring device for livestock insemination, frozen semen tubes can be kept in a frozen state and are convenient to transport and store, and the thin tube supports can play a role in protecting the frozen semen tubes.

The utility model provides an artificial insemination table for breeding cattle and sheep, and relates to the field of artificial insemination. The artificial insemination table for cattle and sheep breeding comprises a fixing base and a supporting table, a connecting frame is fixedly connected to the bottom face of the supporting table, a body limiting mechanism for fixing the body is arranged on the surface of the fixing base and comprises a supporting frame and a binding belt, a fixing rod is hinged to the surface of the supporting frame, and the binding belt is arranged on the supporting frame. Limb fixing mechanisms for fixing four limbs are arranged at the two ends of the connecting frame. According to the artificial insemination table for cattle and sheep breeding, the livestock body is supported through the supporting table, the livestock body is fixed to the surface of the supporting table through the binding belt, the neck of the livestock is supported and fixed through the supporting frame and the fixing rod, the phenomenon that the head of the livestock swings at will during an operation is avoided, and the connecting sleeve is fixed through the connecting frame; and then the connecting blocks drive the fixing belts to move to fix the four limbs of the livestock, the four limbs of the livestock are prevented from swinging, and the artificial insemination efficiency is improved.

The invention discloses a perinereis aibuhitensis triploid induction method, which comprises the following steps: placing perinereis aibuhitensis fertilized eggs in ice water for cold shock treatment, then carrying out gradient heating to room temperature, selecting sexually mature perinereis aibuhitensis for artificial insemination, cutting female and male perinereis aibuhitensis into pieces in a stainless steel metal filtering cylindrical net in sequence, and carrying out freeze drying to obtain the perinereis aibuhitensis triploid. According to the method, the stainless steel metal filtering cylindrical net is used for assisting the artificial insemination of the perinereis aibuhitensis, the triploid of the perinereis aibuhitensis is induced through cold shock treatment, the perinereis aibuhitensis induction rate is identified to be 80% or above by means of a flow cytometry and tissue slices, the operation is simple, the cost is low, and the method is suitable for large-scale popularization and application. The cost is saved, and the method is effective and can be used for inducing the marine annelids triploid.

Artificial cervical fluid is disclosed that contains a mucilaginous extract from the okra plant. The mucilaginous extract can be produced using a hot aqueous extractant or cold extraction process followed by separation of larger particles from the extract. The extract finds many uses, for example as a sperm storage medium, a sperm freezing medium, a sexual lubricant, an artificial insemination medium, and an in vitro fertilization medium.

The invention provides an artificial propagation method of schizothorax wangchizoides, and belongs to the technical field of fish propagation. The artificial propagation method of the schizothorax wandersoni comprises the following steps that (1) mature parent fish is selected and injected with an oxytocic mixture, and the oxytocic mixture comprises DOM, HCG and LRH-A3; (2) collecting ova and sperms after the parent fish is oestrus, and carrying out artificial insemination; and (3) transferring the fertilized eggs into an incubator for incubation. According to the method, 268345 artificial fertilized eggs are obtained, the fertilization rate reaches 95.6%, 251707 pond fries are obtained after artificial incubation, the hatching rate is 93.8%, and the fertilization rate and the hatching rate of the schizothorax wandersoni are remarkably increased.

Methods, uses and compositions including FSH for use in the treatment of infertility by intrauterine insemination are described.

The invention provides a method for efficiently inducing larimichthys crocea triploid by using hydrostatic pressure, which comprises the following steps of: immediately transferring fertilized eggs into a hydrostatic pressure device 2 minutes and 30 seconds after artificial insemination of larimichthys crocea sperm eggs, applying pressure of 7000-8000 PSI, and performing hydrostatic pressure treatment for 3 minutes. Wherein in the hydrostatic pressure treatment process, it should be ensured that the pressure is stable, and the fluctuation range does not exceed + / -1%. According to the method, the time and pressure of hydrostatic pressure treatment are accurately controlled, the triploid induction rate can be 90% or above, and the induction rate is high. The damage to fertilized eggs is reduced through optimized processing parameters, the hatching rate of the fries can reach 70% or above, and the hatching rate is high. In addition, the hydrostatic pressure treatment time is only 3 minutes, the operation is efficient, the method is more suitable for large-scale production compared with other methods, and the triploid cultivation efficiency is improved.

The application provides a dairy cow mating auxiliary device, which comprises two symmetrically distributed side frames, a front frame capable of clamping a neck of a cow is fixed between one end of the two side frames, the other end of one of the side frames is provided with a clamping ring with a circular ring structure, the ring wall of the clamping ring is a hollow structure, the inner ring wall of the clamping ring is made of elastic rubber material, and the ring wall of the clamping ring is connected with a gas pressure adjusting device capable of adjusting the gas pressure in the ring wall. In the artificial insemination process, the labor intensity of the staff can be reduced without artificial hand pulling of the cow tail, and the stability of the cow tail clamped by the inner ring wall is relatively high, so that the cow tail cannot break away from the clamping ring even if the cow resists and swings the tail.

The invention relates to the use of carbon monoxide for preserving gametes. Means and methods for preserving gametes are provided. The method of the invention comprises the steps of a) providing a gamete or a sample comprising said gamete in a container, and b) contacting said gamete in said container with carbon monoxide. The invention also provides the use of carbon monoxide in the treatment and / or prevention of diseases, such as diseases caused by or associated with elevated DNA cleavage, redox potential of gametes, and / or diseases of gametes caused by or associated with elevated ROS levels. Also provided are uses of carbon monoxide for the prevention of congenital abnormalities and / or aneuploidy, as well as for the treatment of diseases caused by and / or associated with elevated DNA cleavage and / or redox potential of gametes. The invention also relates to a method of treating congenital abnormalities or aneuploidy comprising contacting carbon monoxide gas with gametes of a patient in need thereof. Thus, the means, methods and uses of the present invention ensure that gametes are preserved in a functional / intact state, thereby ensuring maintained and / or improved gamete quality. Thus, such carbon monoxide treated / carbon monoxide exposed gametes are particularly useful in reproductive techniques, such as in vitro reproductive techniques, including in human assisted reproductive techniques and / or in animal (artificial) insemination techniques.

The invention relates to an artificial insemination device (1) for livestock, in particular pigs, comprising a tubular body (2) and a tip (3) secured at one end (6) of the tubular body (2) and configured to be inserted and positioned in the cervix of a female animal, characterized in that the tip (3) is composed of at least agar-agar, water and glycerol. figure for the abstract: 3