627 results about "Juvenile fish" patented technology

The invention relates to a water circulation system and a method for breeding marine fishes inland, and mainly solves the technical problems of complex process, large investment, high operating cost, large limitation of breeding varieties, low survival rate, slow growth and the like in the process of breeding the marine fishes in fresh water or low salinity salt water at present. A technical scheme comprises the following steps of: a, establishing the water circulation system; b, performing domestication on juvenile fishes of the marine fishes in the low salinity salt water in a gradient mode, namely breeding the juvenile fishes which are bred in the seawater in the brackish water temporarily, formulating a domestication strategy of slowing salinity gradually, and performing domesticationon the juvenile fishes of the marine fishes through three gradients, namely the brackish water phase, the low salinity phase and the ultra-low salinity phase; c, adding a physiological salt preparation, namely adding metal ions which play a key role in osmoregulation into the water or a feed; and d, managing the breeding, namely providing a preference temperature, illumination intensity and a daily illumination period. The water circulation system and the method are mainly used for breeding the marine fishes inland.

The invention relates to compound feed suitable for juvenile fish of myxocyprinus asiaticus, which is characterized by comprising the following components in percentage by weight: 40 to 45 percent of crude protein, 10 to 14 percent of crude fat, 20 to 25 percent of total sugar, 0.70 to 0.85 percent of available phosphorus, 6 to 10 percent of moisture, 2.0 to 2.5 percent of composite vitamin, 1.5 to 2.0 percent of composite mineral salt, 0.3 to 0.5 percent of immune potentiator, 0.3 to 0.5 percent of antioxidant and 2 percent of adhesive. The feed comprises the following raw materials in percentage by weight: 60 to 75 percent of protein source raw material, 8 to 10 percent of fat source raw material, 10.0 to 15.0 percent of sugar source raw material, 2.0 to 2.5 percent of composite vitamin, 1.5 to 2 percent of composite mineral salt, 0.3 to 0.5 percent of immune potentiator and 0.3 to 0.5 percent of antioxidant. The compound feed of the invention solves the problem of unbalanced nutrition of the current compound feed for feeding the juvenile fish of the myxocyprinus asiaticus, and reduces the environment pollution during feeding.

Larviculture is performed using Parvocalanus sp as a feed for fish larvae. A system is described using tanks for growing Parvocalanus sp nauplii with a microalgae feed and transferring the grown Parvocalanus sp nauplii to tanks containing the fish larvae, where the functions of the tanks is interchanged. The Parvocalanus sp feed provide for higher numbers of larger juvenile fish and the rearing of larvae heretofore not reared in culture.

The invention provides an alga / fish mixed type three-dimensional ecological fish shelter and a feeding method of the fish shelter, wherein the fish shelter comprises an anchoring system, a fish shelter top consisting of a floating ball and a fixed frame, a fish shelter middle layer consisting of a horizontal grid and a fixed frame suspended with a middle spherical fish shelter, and a fish shelterbottom consisting of a horizontal grid and a fixed frame suspended with a bottom spherical fish shelter; and the fish shelter middle layer is respectively connected with the fish shelter top and the fish shelter bottom by a connecting rope and the lengthways crossed grid. The feeding method comprises the following steps of: shipping to a feeding sea area, firstly throwing a first anchor along with the direction of incident flow, sequentially throwing a second anchor by means of fair current, tying an anchor tail rope and a mark float at the back end of the anchor, dragging the anchor tail rope by means of fair current, tensioning a whole system, and completing feeding. According to the invention, the fish shelter can be used for culturing and proliferating the alga with different water depths, is taken as a perching and refuge occasion of the small size medium-upper layer fish and a large size medium-upper layer juvenile fish, and is simple in structure, and convenient to assemble andrecover, so that the novel artificial fish shelter can be provided for building an ecological marine ranch.

A method for culturing the minnow of roughskin sculpin includes such steps as capturing the parent fish in near sea in early spring, temporarily culturing in indoor pool, using flowing water to stimulate the parents for estrus, natural mating, ovipositing, collecting fertilized ova, natural incubating in natural seawater in pool, regulating illuminance for correcting the strong phototaxis, applying nutritive forage, improving water quality, and post culturing.

An industrial breeding method for banded grouper includes such steps as intensified culture of parent grouper, oviposition, incubation, culturing fry and culturing young banded grouper. Its advantages are strong young banded grouper, and high operatability.

The invention publishes a coreius heterodon fry collection, holding culture and long-distance transportation method. The method comprises the following steps of: selectively performing bottoming and downstream fishing in a water area of relatively concentrated coreius heterodon juvenile fishes with water depth of 10 m to 20 m, slow water flow and a back water area by using a small-sized fish bottom trawl during 10-11 months after water temperature is lower than 25 DEG C; screening out coreius heterodon fries with complete body surfaces and better activity from catches, performing body surfacedisinfection and then transferring into a live fish carrier cabin to perform holding culture for 2 days, wherein the flow rate of a holding culture water area is 0.5 m / s to 0.8 m / s, the water level is kept at 50 cm to 70 cm, and the water temperature does not exceed 25 DEG C; and after the holding culture is finished, filling the coreius heterodon fries into a fish fry bag with water, meanwhile, placing ice blocks, filling oxygen according to a water-air volume ratio of 1 to 1 and then sealing the neck of the bag, and filling in a foam box with a cover to transport away from sunshine, whereinthe entire carriage is controlled within 8 hours. The method is suitable for long-distance transportation of coreius heterodon fries; secondary aeration is not required halfway; and the survival rateis high.

The invention discloses an artificial breeding method for siganus oramin. Siganus canaliculatus and Siganus fuscesens are common fish stocks of Siganus, the physiological characters, reproductive habits and the breeding methods of the Siganus canaliculatus and Sigauns fuscesens are more similar, the problems that the source and domestication are difficult, newly hatched individuals are small in body size, the death rate caused by strong irritability of larva is high, a systematic artificial breeding technique is in shortage, the breeding offspring seeds are mainly from natural seedlings captured from ocean, and the like exist in the artificial breeding process. The artificial breeding method adopts the technical measures of parent fish culturing, cultivation, artificial spawning and incubation, larva cultivation pool preparation, cultivation water quality conditioning, staged bait feeding and juvenile fish transportation, so that the industrialization production of parent fish supply and seedling culture is realized, and the technical problems of artificial spawning and incubation of parent fish, selection and enrichment of larvae bait, water quality regulation and control, larva transportation and the like in the artificial breeding process of Siganus canaliculatus and Siganus fuscesens are systemically solved. The spawning rate of the cultivated parent fish can reach more than 65 percent, the average spawning fertility rate can reach more than 85 percent, and the survival rate of artificial breeding is 10.7 percent-27 percent.

The invention discloses a feed proportioning method and a cultivation method of juvenile yellow catfish. The proportioning method is characterized in that a juvenile yellow-head catfish feed mainly comprises 9.8-43.2wt% of vegetable protein, 34-8.8wt% of animal protein and the balance of an auxiliary feed. The cultivation method of the yellow-head catfish meat mainly comprises the step of: alternately feeding the juvenile fish by a juvenile fish feed with high-specific gravity animal protein and a juvenile fish feed with low-specific gravity animal protein at time intervals. The conventional feeding mode only uses a feed with single specification in the cultivation of the juvenile yellowcatfish. The invention makes use of the characteristic of the compensation growth of the yellow catfish, and the juvenile fish feed with the high-specific gravity animal protein and the juvenile fish feed with the low-specific gravity animal protein are alternately fed at time intervals, so that the feed efficiency can be improved, and the feed cost can be saved on the premise that the weight increment of the yellow catfish is not influenced.

The invention discloses a self-submergence frame type sampling method of marine juvenile fish by a sampling net, which relates to a sampling method of marine juvenile fish and needs to provide a net tool for sampling the marine juvenile fish away from the bottom and a method for controlling sampling depth. The sampling net tool provided for the sampling method is characterized in that a net port of the sampling net tool is welded into a rectangular frame by a steel pipe; four non-section net pieces with meshes of 10mm are cut into trapezoidal net pieces which are wound into shapes of a net bag; a wide port of the net bag is engaged with the rectangular frame; a narrow port of the net bag forms a bag net; the tension length of netting of the net bag is 4 meters; the lower edge of the net port is connected with a submergence device which is made into a framework by the steel pipe; a submergence deflector covers the upwind surface of the framework of the submergence device; both ends of the submergence device are hinged with triangular drawing plates which are connected with a cross rope; upper transverse beams of the net port are all uniformly tied with floaters; ship speed is between 3 knots to 3.5 knots during net placement; and the length of the cross rope is controlled and released gently by operating water depth so that the net tool is placed on an operating water layer to draw the net and sample. The sampling method is suitable for sampling the marine juvenile fish.

A feeder for larval and juvenile fishes is capable of delivering a small (ca. 15 mg) precise dose of microparticulate (ca. 100 μm diameter) feed to selected locations, via pneumatic conveyance and control. A source of low-pressure dry gas is used to blow microparticulate feed through a manifold and into a selected one of a number of tubes. A terminal valve at the end of the tube is selectively activated to send the feed to a selected tank or pond. When not in use, the feeder is sealed, and the feed protected from moisture and ambient oxygen.

The invention discloses a full-artificial breeding method for megalonibea fusca. The method comprises the following steps of: getting parent fish seeds from natural sea areas; carrying out reinforced breeding on parent fish; carrying out artificial spawning on the parent fish; carrying out hatching on fertile eggs; carrying out indoor artificial cultivation on juvenile fish; carrying out indoor nutrition strengthening cultivation on the juvenile fish in an alevin stage; carrying out ecological breeding on the alevin which are moved into an earthen pond after the alevin stage; and breeding thealevin into large-size seedlings, and the like. The full-artificial breeding method provided by the invention is a mode applicable to the large-scale production of megalonibea fusca fries. After carrying out three-year cultivation on first-generation juvenile fish which is successfully artificially bred, sexually-matured first-filial-generation parent fish is cultivated, and then second-generation juvenile fish is bred from the first-filial-generation parent fish, thereby achieving the full-artificial breeding. The full-artificial breeding method breaks through the bottleneck that because themegalonibea fusca belongs to endangered fish species, parent fish sources are extremely difficult, and realizes the large-scale artificial breeding and culturing industrialization of the megalonibea fusca.

The invention relates to an indoor cement pond culture technique of Yangtze River Coilia ectenes, belonging to the field of Coilia ectenes culture. The indoor cement pond culture technique of the Yangtze River Coilia ectenes comprises the steps of fingerling acquisition and transportation, temporary culture and domestication, indoor culture and overwintering culture. The indoor cement pond culture technique of the Yangtze River Coilia ectenes is characterized in that juvenile fish with body length being 8-12cm is acquired in September to October and is put in an indoor cement pond for temporary culture; after the juvenile fish is domesticated for 2-4 weeks, the juvenile fish is transferred into an indoor small cement pond for culture; when the juvenile fish is cultured to November to December of each year and the water temperature is below 15DEG C, the Coilia ectenes is transferred for the overwintering culture, the overwintering culture of the Coilia ectenes is conducted at room temperature and the water temperature is kept above 8DEG C to keep the juvenile fish to intake food; during overwintering, live seawater copepods are used as fish feed, and if the live fish feed is not enough, the live fish feed can be replaced by frozen opossum shrimps or slow-sinking compound feed; and after the spring of the next year begins, the juvenile fish is cultured according to the indoor culture method.

The invention relates to an artificial particle feed for juvenile turbot. The artificial particle feed is characterized by being prepared from whitefish powder, euphausiid powder, scallop powder, beer yeast, starch, cod liver oil, yolk powder, whey powder, L-alanyl-L-glutamine, choline chloride, soybean lecithin, schizochytrium limacinum powder, haematococcus pluvialis powder, compound vitamins, compound mineral substances, Chinese herbal medicines and sodium alginate. As the various raw materials are added into the feed, the comprehensiveness of nutrition is guaranteed. An immunopotentiator is added into the feed, so that the non-specificity immunity of fish bodies is enhanced. The feed is green and pollution-free. The schizochytrium limacinum powder and the haematococcus pluvialis powder are added into the feed, and the content of vitamin E is improved, so that the whitening rate of the juvenile fish is effectively reduced. The Chinese herbal medicine components are added, so that the immunity of the juvenile fish can be improved and parasite diseases in a juvenile fish growing phase are prevented from happening.

The invention relates to the technical field of animal feeds, and more specially relates to a feed for juvenile grass carps. The feed comprises the following components in parts by weight: 10-16 parts of bean pulp, 16-20 parts of wheat middling, 16-38 parts of straw powder, 8-12 parts of rapeseed meal, 12-16 parts of fish meal, 0.1-1.0 part of multi-vitamin, 2-5 parts of a traditional Chinese medicine additive and 0.5-1.5 parts of sesame oil, wherein the traditional Chinese medicine additive comprises the following components: honeysuckle, folium isatidis, common andrographis herb, golden cypress, coptis chinensis, codonopsis pilosula, glossy privet fruits, mung beans, hawthorn, astragalus membranaceus, medicated leaven, poria cocos, pericarpium citri reticulatae, Chinese yams and roasted malt. According to the feed provided by the invention, the survival rate of the juvenile grass carps and the weight of adult fish are improved, the survival rate can reach 99%, and the growth rate of body mass further can reach 174.6%, and the feed coefficient is reduced, the feed conversion rate is improved and the benefit of companies is increased.

The invention provides a method for cultivating scatophagus argus in an indoor cement pool, and relates to scatophagus argus cultivation. The method comprises the steps of yearling cultivation, overwintering cultivation and adult fish cultivation, and is characterized in that juvenile fish is firstly placed in a cement pool net cage in a vinyl house for temporary rearing after transported to the destination, the adapted juvenile fish is placed in a cement pool for domestication cultivation, cultivation water adopts natural seawater in river mouth district, the salinity is 8-15, the cultivation water is filtered by 200-mesh bolting silk after secondary precipitation, the cultivation is performed until November to December, the water temperature is lower than 12 DEG C, scatophagus argus is transferred to the indoor cement pool for overwintering cultivation, seawater buoyancy No. 2 grains are fed by two times every day according to 1%-3% of fish weight during the overwintering cultivation, and the water temperature during the overwintering cultivation is kept higher than 12 DEG C; and the water temperature in the spring of next year is increased to be higher than 15 DEG C, yearlings are transferred to the cement pool in the vinyl house from the indoor cement pool for adult fish cultivation, the body length of adult fish is up to 8-10 cm, pool separation is performed, and fresh enteromorpha or filamentous algae are fed during daily management period.

Disclosed is an artificial seed production and seedling method of tilapia, the method comprises the steps of parent fish breeding and cultivation, induced spawning and ova picking, artificial incubation, seedling cultivation process. The parent fish breeding is at lease implemented twice, when young fish is cultured to reach the weight of 50 to 100 g and before overwintering, the female and male parent fish is chosen by the proportion of 2:1 to 1.5, and respectively put into a cultivation e pond to culture according to different genders. Mature tilapia parent fish is put into an induced spawning pond, and the natural estrus and spawning of the parent fish are promoted by utilizing the ecological simulation method, fertilized eggs are taken out from oral cavity of the female fish and removed to a hatching slot to hatch, then the fertilized eggs are impacted by micro water current to roll and hatch completely in the hatching slot, after newly-hatched larva is flowed into a receiving pond and is screened by genders, the newly-hatched larva is sent into a breeding pond to culture. The method is scientific and reasonable, the technology is easy to be controlled, the investment is low, the facilities are simple, the method is easy to be implemented, the artificial breeding of tilapia is realized. The method has the advantages that the breeding time is short, the fish fry quality is good, the survival rate is high and the like, the method is applicable to large-scale breeding production, and has the good economic benefit.

The invention discloses a Hippocampus kelloggi ecological breeding method. The Hippocampus kelloggi ecological breeding method comprises the steps of previous preparation, ecological breeding model establishment, newborn Hippocampus kelloggi breeding, Hippocampus kelloggi juvenile fish breeding and Hippocampus kelloggi adult fish breeding. The previous preparation includes reconstructing a shrimp seed breeding pond into a Hippocampus kelloggi breeding pond, and filling the pond with water after the pond is completely cleaned; ecological breeding model establishment includes in a microalgae cultivation stage, cultivating microalgae in water 3-5 days before Hippocampus kelloggi breeding, utilizing the microalgae as feedstuff of rotifers, artemia and fairy shrimps and improving water quality through photosynthetic bacteria; the newborn Hippocampus kelloggi breeding includes firstly measuring and controlling water quality, namely, detecting various indicators before stocking, then dividing the breeding stages into a breeding stage less than 15 days and a breeding stage between 15-30 days according to the duration of breeding days, and properly controlling the stocking density and feedstuff components of every breeding stage. The Hippocampus kelloggi ecological breeding method and artificial breeding method provide technical support and theoretical foundations for large-scale commercialized Hippocampus kelloggi breeding. By means of the Hippocampus kelloggi ecological breeding method, the breeding cost is reduced by 50%-60%, the quality of the breeding quality can be easily controlled by detecting various indicators before stocking, and high survival rate and growth rate of Hippocampus kelloggi can be guaranteed.

A composite feed for the young turbot is prepared from 14 raw materials including anabarilius powder, spirulina powder, concentrated soybean protein, squid powder, beer yeast, soybean lecithin, etc. Its advantages are rich nutrients, high conversion rate, high growth speed, and less pollution to water.

The invention discloses a graded culture method for large yellow croaker fry, which comprises the following steps of: performing fertilized egg incubation and membrane broken juvenile fish culture in an indoor nursery pond; when 20 percent of juvenile fish is cultured to reach the body length of 20 millimeters, training the juvenile fish by using a proper amount of eel powder and feed in the morning; when 50 percent of juvenile fish is cultured to reach the body length of 20 millimeters, performing reinforced training for 3 days by using full eel powder; transferring the fry with body length of not less than 20 millimeters to an outdoor secondary net cage for culture, wherein the culture density of the secondary net cage is 90 to 110 thousand pieces; transferring the fry with body length of not less than 30 millimeters to an outdoor tertiary net cage for culture, wherein the culture density of the tertiary net cage is 50 to 70 thousand pieces; transferring the fry with body length of not less than 40 millimeters to an outdoor quaternary net cage for culture, wherein the culture density of the quaternary net cage is 30 to 40 thousand pieces; and when 95 percent of fry is cultured to the body length of not less than 50 millimeters, obtaining the large yellow croaker fry capable of being put to breed. The graded culture method for the large yellow croaker fry has regular fry specification, high fry growth speed, high survival rate and good adaptability of the fry to the culture environment.

The invention relates to a large-scale natural propagation method for artificially culturing siganus guttatus parents, and the method comprises the following steps: after 1.5-3cm juvenile fish grows into parent fish under an artificial culturing condition, boosting the healthy growth of the parent fish by utilizing the natural propagation of an artificial regulating and controlling technology, optimizing the parent fish, optimizing the culturing environment of the parent fish during the culturing process and optimizing baits; in an antenatal stage, suitably adding the natural baits and suitably changing the culturing environment, thereby boosting the gonad maturity of the parent fish; and naturally purifying the culturing environment of the parent fish in the antenatal stage and disinfecting the culturing environment of the parent fish in a postpartum stage, thereby ensuring the survival of the parent fish, increasing the output of the juvenile fish, providing a large number of high-quality fish fries for the industrialized development of the siganus guttatus culturing, and meanwhile, increasing the survival rate of the artificially cultured parent fish and the survival rate of the juvenile fish.

The invention relates to the technical field of aquaculture and particularly relates to a novel feed for juvenile fish of pelteobagrus fulvidraco. The feed is composed of, by mass, 20-40 parts of brown fishmeal, 15-20 parts of flour, 5-10 parts of flour, 15-20 parts of soybean meal, 4-8 parts of peanut bran, 5-10 parts of rapeseed meal, 15-20 parts of corn protein powder, 1-2 parts of soybean oil, 1-3 parts of fish oil, 0.5-1.5 parts of monocalcium phosphate, 0.5-1 part of composite vitamins, 0.5-1 part of composite minerals, 2-3 parts of Chinese herbal medicines, 0.2-0.5 parts of an organic acid phagostimulant, 0.3-0.7 parts of choline chloride, 0.1-0.3 parts of a mildew proofing agent, and 0.2-0.4 parts of an antioxidant. In the invention, Chinese herbal medicines are added to a conventional pelteobagrus fulvidraco feed, so that survival rate of the juvenile fish of pelteobagrus fulvidraco is effectively increased, darkened body color is prevented and bright body color under a wild status of the pelteobagrus fulvidraco can be maintained, thereby improving quality of the juvenile fish. Meanwhile, the Chinese herbal medicines has spectral sterilization effects, so that pathogenic bacteria in water body can be killed effectively, thereby improving situation of water body, increasing the survival rate of the pelteobagrus fulvidraco, reducing application of chemical reagents and reducing breeding cost.

The invention relates to a herring smelt juvenile fish feed. The herring smelt juvenile fish feed comprises the following materials in percentage by weight: 35%-39% of fish meal, 1%-3% of dried small shrimps, 3%-7% of yeasts, 6%-10% of expanded soybeans, 3%-7% of phospholipid powder, 5%-9% of flour, 5%-9% of lactalbumin powder, 1%-5% of skim milk powder, 2%-6% of wheat germ meal, 11%-15% of fish oil, 1%-5% of soyabean oil, 1%-3% of palm oil, 1.5%-2.5% of alfalfa meal, 0.05%-0.15% of vitamin C, 0.5%-0.7% of vitamin B complexes, 0.15%-0.45% of vitamin D, 0.02%-0.06% of carotenoid, 0.5%-1% of choline chloride, 0.01%-0.02% of taurine and 0.1%-0.2% of emulsifiers. According to the herring smelt juvenile fish feed disclosed by the invention, the requirement of the herring smelt juvenile fish onnutrition is met, and energy needed by the growth and the development of the herring smelt juvenile fish is provided.

The invention relates to an artificial mixed breeding method for spotted maigre and large yellow croaker in marine aquaculture industry, and belongs to the technical field of marine culture. The breeding method includes the following steps, firstly pond-separated breeding maturation accelerating and parturition hastening of parent fish; secondly, same-pond breeding of spotted maigre and large yellow croaker fry; and thirdly, fry separating that's carried out through siphonage after the fry grows to be 2 to 3 cm in length. The pond-separated breeding maturation accelerating and parturition hastening of parent fish includes the maturation accelerating of the spotted maigre and the maturation accelerating of the large yellow croaker, and both the maturation accelerating of the spotted maigreand the maturation accelerating of the large yellow croaker include parent fish breeding and gonad maturation accelerating and parturition hastening. The same-pond breeding of spotted maigre and large yellow croaker fry includes same-pond hatching of the fertilized eggs of the spotted maigre and the large yellow croaker, prelarva prior period growing management, prelarva later perior growing management, postlarva period growing management and water quality regulation and control. 1.5 kg of fertilized eggs of the spotted maigre and the large yellow croaker are thrown into a mud pond of 32m<2>,and 696, 7000 juvenile fishes of 2 to 3 mm are bred at last, and the breeding rate per unit area is 2300 / m<2>, as a result, the utilization rate of the breeding pond is greatly improved and the breeding cost is reduced.

The invention relates to a device and a method for actively stimulating juvenile fishes to escape. The device can be applied to sac-type fishing gears such as a trawl and a trap net, and comprises a cylindrical cod-end consisting of a front rope loop, a funnel net, a rope loop, a square mesh section, a rear rope loop and meshes, wherein the cylindrical cod-end is connected onto a net body to form the whole trawl. The device is characterized by further comprising a luminous circular ring arranged in the middle of the cod-end, four fixing holes are formed in the inner periphery of the luminous circular ring and connected with the rope loop through four cords, and the horizontal distance between the rope loop and the luminous circular ring ranges from 20cm to 30cm. In order to further drive the juvenile fishes, a conical rope array comprising at least three hollow small bells and a plurality of ropes is arranged on the periphery of the luminous circular ring. Obviously, the juvenile fishes can be actively stimulated to escape the trawl, juvenile fish resources can be protected, and the device is simple in structure, easy to manufacture and low in material cost.

A formulated, microbound diet product for the culture of larval fish and crustaceans either in a dry or moist form is disclosed. The food product contains protein sources such as fish protein hydrosylate, casein, egg yolk, binding agents such as soy lecithin, wheat gluten, and alginate. Other ingredients such as vitamins and minerals, lipid sources, carbohydrate sources, pigment sources, and attractant compounds are included in the diet for nutritional completeness. A method for preparation of the food product is also disclosed.

The invention discloses a method for hybridizing female verasper variegatus and male verasper moseri for breeding and fry cultivating, comprising the steps of selection of parents, parent fish cultivation and reproduction control, artificial insemination and incubation and cultivation of fish larvae and juvenile fish, wherein female verasper variegatus older than 3 years is selected to be used as a female parent, and male verasper moseri older than 2 years is selected to be used as a male parent; and the sexual maturity synchronization of the female parent fish and the male parent fish is promoted by means of temperature control, light control and nutrition enhancement. With respect to the artificial insemination, the insemination rate reaches 50%; the water temperature for incubation is (10+ / -0.5) DEG C, fish larvae can be incubated from inseminated eggs after 160 hours, and the incubation rate reaches more than 50%; and with respect to a feed series, L-type brachionus plicatilis and chlorella sp., artemia nauplii, and compound feed are respectively applied in different stages. The fish larvae (1-35 days in age) in an early stage are cultivated in a small water body (3m<3>) at the water temperature of (10+ / -0.5) DEG C; after 3 days, the temperature is raised by 0.5 DEG C every 2 days and is kept unchanged after reaching (15+ / -0.5) DEG C; and the fish larvae in a later stage are cultivated in a water body (30m<3>). After cultivating for 80 days, the survival rate of the fish larvae growing into the juvenile fish of 30mm in average is 20%. The method disclosed by the invention can be used for cultivating novel breeds and realizing scale breed reproduction.

The invention relates to a method for realizing high-yield clown fish parent fish culture in running water, which comprises the following steps: classifying clown fishes from a productbility angle and respectively selecting suitable culture containers; selecting wild clown fish parent fishes or juvenile fishes for artificial pairing; strengthening the simulation of natural environments for egg laying of parent fishes, including the simulation of habitat, water flow and illumination; selecting different maturing baits for maturing the clown fishes according to the different types of clown fishes; and strengthening management during incubation period and reaching the effects of high egg laying amount and high hatching rate of the cultured clown fish parent fishes in a running water culture system. The invention solves the existing common problem that the egg laying amount and the hatching rate of the clown fish parent fishes are low, improves the situation that the culture yield of the clown fishes is low and is of great importance to the industrialized development of clown fish culture.

The invention discloses a soft pellet large yellow croaker and juvenile fish compound feed which comprises the following components: fish meal, pre-gelatinized starch, extruded soybeans, fermented soybean meal, precision corn protein, fish oil, beer yeast, mineral substances, monocalcium phosphate, choline chloride and multivitamin. Compared with traditional feed, the large yellow croaker and juvenile fish compound feed has the advantages of comprehensive nutrition, less pollution to culture water bodies and wide raw material source; and compared with the prior expanded pellet feed, the soft pellet compound feed has the advantages of high feed conversion rate, high digestibility and utilization rate, convenience in production, greenness and nuisanceless, and the like.

The invention discloses a primer and a method for quickly detecting gender difference of juvenile fishes of fugu rubripes. A pair of outer PCR (Polymerase Chain Reaction) primers which respectively amplify gender difference gene sequences and an inner PCR primer which contains mispaired basic groups aiming at mutation sites are designed aiming at G-C mononucleotide mutation sites existing on gender differential genes of male and female individuals of fugu rubripes. By means of the primer, different amplification results can be obtained by different gender DNA (Deoxyribonucleic Acid) samples of fugu rubripes, so that the gender difference of fugu rubripes can be judged. The primer disclosed by the invention is low in cost, simple to operate, quick and accurate and suitable for popularization and application, can quickly identify gender of juvenile fishes of fugu rubripes on a basis of extracting genome DNA of fugu rubripes one year old, and has important application value to accelerate the unisexuality breeding process of fugu rubripes and PCR detection method research in the field of molecular biology.