96 results about "Juvenile fish" patented technology

The invention provides an AI-based trachinotus ovatus juvenile fish culture water parasite prediction and prevention method and system, and the method comprises the steps: collecting multi-source data from a culture water body through a sensor array, including a nucleic acid signal, a fish school behavior track and an environment parameter sequence, and obtaining an original data set; unifying the data format and the acquisition frequency according to the original data set by adopting a time sequence alignment method, and obtaining an aligned feature flow; aiming at the aligned feature flow, applying a convolutional neural network to extract a space-time pattern of a nucleic acid signal and an abnormal index of a fish school behavior, and determining an extracted feature set; if the abnormal index in the extracted feature set exceeds a preset threshold value, a causal association between environmental parameters and parasitic risks is classified through a support vector machine, and a preliminary risk level is judged; simulating a water body change scene according to the comprehensive causal model to obtain a predicted trajectory of parasite propagation; and determining a quantitative score of the parasite risk and generating a prevention and control instruction sequence by comparing the predicted trajectory with the real-time monitoring data.

The invention belongs to the technical field of aquatic genetic breeding, and particularly relates to a grass carp disease-resistant character genome breeding method and related application thereof. According to the method, filial generation juvenile fish families produced by non-family grass carp parents from different geographical sources are used for constructing an anti-hemorrhagic disease reference group, the disease-resistant SNP site provided by the invention is used for calculating a genome estimation breeding value of a candidate group, and individuals with high breeding value are relatively high in disease resistance and can be used for breeding disease-resistant improved varieties of grass carp.

This invention discloses a multi-unit composite stress tiered breeding device and method for juvenile large yellow croaker, belonging to the field of aquaculture breeding technology. The device uses a central control unit as its sole core, forming a fully integrated unidirectional closed-loop drive system. Four independent breeding units are connected through a transparent channel with a centrally located aeration head, forming a physical flow-limiting isolation structure. Each unit independently regulates water temperature, salinity, and dissolved oxygen, creating a multi-factor composite stress gradient. The breeding method employs a three-factor linear equal-gradient synergistic progressive setting, utilizing the juvenile fish's autonomous adaptation and avoidance instincts to achieve cross-unit migration and natural selection. Combined with contour recognition and dynamic tracking algorithms, it monitors the fish population status and mortality rate in real time, and identifies superior offspring with comprehensive stress resistance through secondary verification under extreme stress. This invention offers high screening accuracy and automation, increasing the survival rate of superior individuals by over 40%, reducing labor costs by over 60%, and allowing for flexible adjustment of device parameters. It can be extended to the stress resistance breeding of other marine economic fish species.

The application belongs to the field of fish development, and particularly relates to a molecular marker for identifying Nibea albiflora sperm-induced Pseudosciaena heteroclita gynogenesis offspring and hybrid offspring, a primer set thereof, a kit and application. The primer set for identifying the molecular marker of the Nibea albiflora sperm-induced Pseudosciaena heteroclita gynogenesis offspring and hybrid offspring comprises primers F and R, the nucleotide sequence of the primer F is shown as SEQ ID NO. 1, and the nucleotide sequence of the primer R is shown as SEQ ID NO. 2. Based on genomic data analysis, the application develops the molecular marker F / R which can effectively identify the Pseudosciaena heteroclita gynogenesis offspring and hybrid offspring in the juvenile stage. The method comprises extracting genomic DNA of parents and offspring, PCR amplification and agarose gel electrophoresis detection, and finally distinguishing the gynogenesis individuals and hybrid individuals by whether the Nibea albiflora parent marker is contained in the electrophoresis band.

The utility model discloses a novel young fish fry seed gathers and captures device, including the casing, its characterized in that: the casing upper end is equipped with the feeding opening, and the bait filter drum is arranged in the casing, and the buffer board is arranged in the casing, and the inside rotation is connected with the deflector in the casing, and the left and right ends of deflector all are equipped with the connecting shaft, and one end of connecting shaft is equipped with the gear, and the upper end rotation is connected with the threaded rod in the casing, and the outside screw thread connection of threaded rod has the U type rod, and the left and right ends of U type rod all are equipped with the connecting rod, and the lower end of connecting rod is equipped with the rack, and the upper end of casing is equipped with the first limit slip mouth, and the inside sliding connection of first limit slip mouth has the filter baffle. The utility model can gather and catch the young fish fry of the elastic coating fish, not only can save manpower, can improve the gathering and catching effect, and can also reduce the harm to the young fish fry when gathering and catching.

The invention discloses a lake ecology appropriate water level determination method based on juvenile fish migration and adult fish fattening, and relates to the field of water ecology and water conservancy projects. The method comprises the following steps: acquiring lake basic data; establishing a two-dimensional hydrodynamic model, and simulating a hydrodynamic field in key periods of migration of juvenile fishes and fattening of adult fishes of the target fishes; constructing a habitat model, and calculating a habitat suitability index HSI and a weighted available area WUA; constructing a water level-WUA response curve and performing segmentation fitting; identifying a WUA stable high-value platform through a first-order derivative threshold value, and determining a suitable water level interval; and establishing scheduling mapping between the appropriate water level and the upstream discharged flow. According to the method, water level-habitat quantitative association is realized according to the staging habitat requirements of four major Chinese carps in the Yangtze River, an operable scheduling threshold value is output, the problems that a traditional method is not staging, the index is single and the method is disjointed with engineering are solved, and the method is suitable for ecological scheduling and management of riverway-communicated lakes.

This invention relates to the field of aquatic compound feed technology, specifically to a special feed for mullet, silver carp, and golden carp cultured in saline-alkali land in Northwest China. The feed is characterized by being composed of the following raw materials by weight percentage: 10%–14% alfalfa meal, 16%–22% soybean meal, 16%–22% corn meal, 5%–7% molasses, 1%–3% yeast powder, 2%–5% lactic acid bacteria preparation, 2%–5% premixed feed for aquatic life in saline-alkali land in Northwest China, 1%–4% fish oil, and 1%–5% vegetable oil. This invention solves the problems of low survival rate, high feed conversion ratio, easy antagonistic inactivation of probiotics, fish oil oxidation, poor water resistance of pellets, and insufficient regional adaptability of traditional feeds. Compared with commercially available ordinary marine fish feeds, the survival rate of juvenile fish is increased by 15% to 30%, the feed conversion ratio is reduced by 10% to 20%, the Vibrio inhibition rate is ≥80%, the power consumption per ton of feed is reduced by 25% to 35%, and the raw material cost is reduced by 30% to 45%. It is suitable for large-scale aquaculture in saline-alkali land in Northwest China and has important value for improving the quality and efficiency of fisheries, ecological restoration, and rural revitalization.

The application discloses a method for rapidly culturing trichodinid, and belongs to the field of aquatic biological technology. Trichodinid widely parasitizes on the body surface of many marine and freshwater economic fishes, is one of main parasitic pathogenic agents of the cultured fishes, is easily transmitted by indirect contact between hosts, causes large-scale death of fry, and brings huge economic losses to fish culture industry. However, trichodinid cannot be cultured in vitro, and its natural infection has seasonal regularity, so that the acquisition of experimental materials of trichodinid has great limitations, the research on trichodinid has been less, and the development of trichodinid disease prevention and treatment drugs and vaccines is hindered. The application achieves the effect that the trichodinid parasitizing on the body surface of the young fish with a certain initial trichodinid infection amount is massively reproduced in a short time by injecting an immunosuppressive agent into the young fish. The method is simple in operation, can significantly shorten the experimental period of trichodinid related research, and is beneficial to the development of drugs and vaccines for preventing and treating trichodinid disease.

The subject of the application is the use of allyl ester of 7-hydroxy-2-oxo-2H-chromene-3-carboxylic acid, represented by general formula 1, for staining bone tissue and mineral structures of larvae of fish of the Danio rerio species in order to assess the development of the skeletal system in biomedical research.

This invention discloses a specific DNA fragment, primers, and applications for sex identification of the longfin sclerotium. PCR amplification and agarose gel electrophoresis were performed using PCR primers. The results showed that individuals with a specific 271bp band were male, while those without a band were female. This invention discloses a molecular marker, primers, and identification method for sexing the longfin sclerotium. Sexing can be determined by taking fin rays without killing the fish; it can also identify the sex of juvenile fish whose sex cannot be determined by appearance. It has advantages such as accuracy, simplicity, speed, and minimal harm to the fish, providing important assistance for the conservation of wild longfin sclerotium resources and the development of the aquaculture industry.

The application discloses a method for regulating body color of ornamental fish and belongs to the technical field of aquaculture. The application finds that, by using thyroid hormone to soak ornamental fish in the fry period and the juvenile fish period, excessive thyroid hormone can significantly reduce the number of red pigment cells and the size of pigment granules of ornamental fish in different periods, and reduce the content of carotenoids; when the synthesis of thyroid hormone is inhibited, the expression of melanin cells and related synthesis pathway genes is promoted, and the carotenoid level is increased. The application first clearly defines the inhibitory effect of thyroid hormone on the development of red pigment cells and the accumulation of carotenoids of gourami, fills the knowledge gap in the field, and provides important guiding information for future regulation of body color and genetic improvement of ornamental fish and other aquatic animals.

The invention relates to the field of submersible type intelligent fish nests, in particular to a submersible type intelligent fish nest which comprises an upper-layer frame body, a lower-layer frame body, a middle frame body, a fixing frame, a recognition assembly, a connecting structure and a folding mechanism, the middle frame body comprises multiple sets of folding pipes capable of being folded, and a placing net is arranged between the upper-layer frame body and the lower-layer frame body; the folding mechanism comprises an electric cylinder and a connecting rope, and the connecting rope is connected with the multiple supporting pipes of the upper-layer frame body. Through the arrangement of the folding mechanism, the whole fish nest is shrunk when big fishes approach, roes and newly hatched juvenile fishes are concentrated in the center of the placing net, meanwhile, gaps of the fish nest are shrunk, the big fishes are not prone to entering the gaps, and the roes and the juvenile fishes are not prone to being caught and eaten. Meanwhile, the contraction action of the whole fish nest also has a frightening effect on big fishes. Therefore, the probability that the fish eggs in the fish nest and newly hatched juvenile fishes are caught by big fishes can be reduced, and the maturity rate of the juvenile fishes and the fish eggs is improved.

The invention discloses a cobitidae fish migration channel simulating natural hydraulic conditions and a design method, and relates to the technical field of fish migration channels, the cobitidae fish migration channel simulating the natural hydraulic conditions comprises a river bank, a water channel is arranged on the river bank, and a dam is transversely arranged in the middle of the water channel. A traditional fishway is mainly designed for economic fishes with high swimming capacity, and a substantive ecological barrier is formed for benthic species with weak swimming capacity such as cobidae fishes. According to the invention, through intelligent linkage of the liftable rockfill dam and the self-adaptive inclined plate, a continuous adjustable hydraulic condition from an almost barrier-free gentle slope to a controllable challenging step is created. Especially in a dry season / juvenile fish period mode, the fishway is converted into a continuous smooth slope, so that juvenile fishes and weak individuals can easily move upwards in a creeping mode close to a natural state, and the migration blocking problem is fundamentally solved.

The application provides an artificial breeding method of Onychostoma wujiangense, and belongs to the technical field of aquatic animal breeding. The artificial breeding method of Onychostoma wujiangense comprises the steps of parent fish classification, parent fish cultivation, artificial parturition induction, natural insemination, artificial insemination, fertilized egg hatching, larva cultivation and juvenile fish cultivation. The parturition induction agent is reasonably matched, the parturition induction injection time is accurate, the parturition induction rate, the fertilization rate and the hatching rate are improved, and the parent fish damage is small. The application also clearly defines the feeding methods at different stages of seed cultivation, improves the survival rate of Onychostoma wujiangense seed cultivation stage, and lays a technical support for the artificial breeding of the endangered species Onychostoma wujiangense.

The invention discloses a fugu rubripes primary hepatocyte efficient separation and culture method suitable for different growth stages, and belongs to the technical field of hepatocyte culture. Through the optimized pre-cleaning and enzyme digestion steps, the difficulty in hepatocyte separation caused by high-fat liver is effectively overcome, stable acquisition of the fugu rubripes primary hepatocytes at different growth stages is realized, dependence on juvenile fish which is not subjected to feed conversion is not needed, and the applicability of experimental materials is greatly widened. According to the method, the cell yield is high, the growth state is stable, and a reliable in-vitro model is provided for fugu rubripes lipid metabolism research and nutritional immune regulation.

The present application belongs to the technical field of compound feed for marine fish, and particularly relates to a feed for improving the antioxidant enzyme activity of juvenile Hexagrammos otakii and a preparation method thereof. The feed for improving the antioxidant enzyme activity of juvenile Hexagrammos otakii is prepared from the following raw materials in mass percentage: 95% to 96.5% of basic ingredients, 1% of compound vitamins, 1% of compound mineral salts, and 1.5% to 3% of an antioxidant enzyme activity composition, and the sum of the mass percentages of the raw materials is 100%; the antioxidant enzyme activity composition is composed of vitamin C and beta-carotene, and the mass ratio of vitamin C to beta-carotene is 1 to 3: 1 to 3. The feed for improving the antioxidant enzyme activity of juvenile Hexagrammos otakii obtained by the present application not only can provide sufficient natural antioxidant components to enhance the antioxidant defense mechanism of the juvenile fish, but also can effectively solve the problems of slow growth, low feed utilization rate, and water pollution of juvenile Hexagrammos otakii caused by insufficient antioxidant activity.

This invention discloses a flow-through culture device for juvenile grouper, in which juvenile grouper are cultured in a rearing pond and multiple biomimetic ponds according to their size. A feeding device is installed on the bank of the rearing pond, including a feeding and sorting section that extends into the rearing pond to collect the fish. A drive unit continuously operates the feeding and sorting section in the rearing pond, continuously collecting fry of different sizes and transporting them to different biomimetic ponds via pipelines. This reduces mechanical damage (abrasions, bruises) and physiological stress on the fish, thereby inhibiting stress-induced diseases and mortality, and improving the survival rate of juvenile grouper during the rearing stage.

The present invention relates to "A process for the production of whitefish from Pátzcuaro (Chirostoma estor) and balanced diets for its growth and fattening." These diets have specific characteristics for this species, with a content of easily digestible protein ingredients that efficiently contribute to larval survival and the size and weight of the juveniles fed in aquaculture farms with the appropriate diet for each stage. These diets can enter the national and international markets due to their unique characteristics and the results obtained with stomachless fish. Additionally, these feeds can be used for the production of species at a lower level of the food chain, which do not require the use of fishmeal and fish oil, making the aquaculture of these species a more sustainable activity.These diets are the first developed specifically for the successful growth and fattening of the species C. estor, "Pátzcuaro whitefish", having the potential to impact its conservation and marketing in the region of geographic distribution.

The invention discloses a comprehensive evaluation method for saline-alkali tolerance of carassius auratus gibelio, which comprises the following steps: performing acute saline-alkali stress on the carassius auratus gibelio and carassius auratus in an embryonic stage, a larva stage and a juvenile stage, performing saline-alkali domestication on a fingerling stage at the same time, increasing salinity and alkalinity once every 10 days in the saline-alkali domestication stage, keeping the up-regulation concentration at a stable maximum value according to fingerling physiological indexes, and obtaining the saline-alkali tolerance of the carassius auratus gibelio. Counting the evaluation value of the saline-alkali tolerance of the carassius auratus gibelio in each stage, determining the weight coefficient of the evaluation value in each stage, then calculating the comprehensive evaluation value of the saline-alkali tolerance in each stage, and comprehensively evaluating the saline-alkali tolerance of the carassius auratus gibelio according to the total value after addition. According to the established saline-alkaline tolerance evaluation system for the carassius auratus gibelio, multiple stages of saline-alkaline tolerance index values are selected for comprehensive weight analysis, the evaluation method is superior to a traditional single-index evaluation method, the tolerance level of the carassius auratus gibelio to saline-alkaline stress can be comprehensively reflected, and basic data are provided for comprehensive evaluation of new germplasm characteristics of the carassius auratus gibelio.

The utility model provides a juvenile fish cultivation device and a three-dimensional juvenile fish cultivation system, and solves the problems that in an existing juvenile fish cultivation system, mechanical groove dividing operation influences juvenile fish growth, and a fixed cultivation groove is difficult to meet the juvenile fish growth requirement. According to the technical scheme, the device comprises a first breeding tank, a second breeding tank, a liquid level adjusting assembly and a sewage draining and filtering assembly; a water outlet and an overflow port are formed in the bottom of the second breeding tank, the first breeding tank is detachably mounted in the second breeding tank, and the bottom wall of the first breeding tank is of a permeable structure; the liquid level adjusting assembly comprises an overflow pipe, and the overflow pipe is arranged in an overflow opening in the bottom of the second breeding tank. The sewage draining and filtering assembly comprises a filter screen and a second connector, the second connector is connected with a water outlet in the bottom of the second breeding tank, and the filter screen is arranged in the second connector.

The invention relates to the technical field of cultivation, and discloses a gymnocyprinus carpio cultivation device which comprises a cultivation pool body, a plurality of sets of programmable water power assemblies are arranged in the cultivation pool body and communicate with a circulating water assembly located outside the cultivation pool body, and the circulating water assembly is communicated with the programmable water power assemblies. The outer wall of the water inlet of the cultivation pool body is sleeved with a non-thermal physical disinfection module. Through the programmable water power assembly, free switching of various flow states such as bottom layer washing laminar flow, whole pool suspension micro-circulation flow and pulse stimulation flow can be achieved in one cultivation pool body. In the spawning period, strong jet flow simulates the scouring environment of a natural riverbed, and the parent fish is effectively stimulated to ovulate; in the mouth opening period, special micro-circulation enables mouth opening bait to suspend through fluid lift force, and the problems that in traditional breeding, bait sinks to the bottom, and juvenile fishes are starved to death are solved; in the ripening accelerating period, the pulse water flow provides necessary movement load.

The invention discloses a triploid rainbow trout juvenile fish feed and application thereof. The formula of the feed comprises 300.0 g / kg of fish meal, 120.0 g / kg of krill meal, 100.0 g / kg of soybean meal, 80.0 g / kg of soybean protein concentrate, 80.0 g / kg of corn protein, 20.0 g / kg of wheat bran powder, 165.4 g / kg of wheat flour and a mixture of different proportions of fish oil and dephenolized cottonseed oil, the proportion of the dephenolized cottonseed oil replacing the fish oil in the mixture is 25%-100%, and 10.0 g / kg of premix, 0.2 g / kg of astaxanthin, 3.0 g / kg of choline chloride, 1.0 g / kg of EDTA, 0.3 g / kg of betaine and 0.1 g / kg of calcium propionate are added. The feed disclosed by the invention is applied to culture of juvenile triploid rainbow trout, and can be used for remarkably regulating fatty acid composition of a fish body, improving muscle texture and enhancing oxidation resistance and heat stress resistance of the liver on the premise of not influencing growth performance, so that dependence on fish oil is reduced, and green, efficient and sustainable development of aquaculture is promoted.

The application discloses a large-specification seawater fry factory grading cultivation system and relates to the technical field of aquaculture. The system comprises a first-stage, a second-stage and a third-stage cultivation pool. The first-stage cultivation pool is connected with the second-stage cultivation pool through a conveying pipeline, and the second-stage cultivation pool is arranged in the third-stage pool. The first-stage pool is provided with an independent first circulating water filtration device and a monitoring unit, and the second-stage and third-stage pools share a second set of circulating water filtration and monitoring units. The monitoring units at all stages are integrated with growth detection, environmental regulation and disease early warning functions. The first-stage pool is used for cultivating newly hatched fry, and when the fry develops to the juvenile stage, the fry is automatically transferred to the second-stage pool through the conveying pipeline; the second-stage pool and the third-stage pool jointly form a mixed culture environment, and when the juvenile fish reaches the target specification, the cage door is opened to transfer the juvenile fish into the third-stage pool. Through the grading environmental control and the automatic transfer mechanism, the system realizes efficient cultivation of fry from fry to large-specification fry, and solves the problems of uneven growth and low sorting efficiency in traditional fry cultivation.

The invention relates to the technical field of aquaculture, in particular to a juvenile fish compound feed ingestion domestication device which comprises a mixing assembly arranged on the bank side of a fishpond, the mixing assembly comprises a stirring part used for mixing biological live baits and compound feed, and the stirring part is installed on the bank side of the fishpond through a supporting part; the discharging end of the stirring part is located above the fishpond, a material scattering assembly used for scattering mixed feed into the fishpond is arranged below the stirring part, and the material scattering assembly moves along the fishpond through an adjusting assembly; the material dispersing assembly comprises a material storing part fixedly connected with the adjusting assembly, and a material distributing part is arranged below the material storing part and arranged above the water surface of the fishpond. Through cooperative work of the supporting part, the stirring part, the adjusting assembly, the storage part and the distribution part, artificial feeding can be effectively replaced in the feed domestication process, the feeding amount of feed can be accurately measured, and the uniformity of the feed scattered into a fishpond can be effectively improved.

The application discloses a device for collecting fry and juvenile fish in the main stream of the Yellow River, which comprises a collecting head, a collecting box, a drainage end and a support, the left and right sides of the collecting box are respectively provided with the collecting head and the drainage end, and the collecting box is further provided with the support, the collecting head is a conical cylinder, the large end of the conical cylinder is provided with a multi-hole conical cover which is gathered away from the conical cylinder, the small end of the conical cylinder is communicated with a water inlet pipe of the collecting box, the drainage end is a drainage pipe on the collecting box opposite to the water inlet pipe, a flowmeter and an axial flow pump are arranged on the end of the drainage pipe away from the collecting box, the collecting box is provided with a one-way self-closing structure and a fry and juvenile fish filtering conical cylinder corresponding to the water inlet pipe and the drainage pipe, and the fry and juvenile fish filtering conical cylinder is provided with an induction cylinder on the front and back sides. The device is used for collecting fry and juvenile fish in an active way, and the food induction cylinder is arranged in the collecting box to attract the fry and juvenile fish to stay, so that the fry and juvenile fish can be efficiently collected, and the fish quality resources in the main stream of the Yellow River can be conveniently and quickly monitored.

The application discloses an assemblable chain type artificial fish reef, which comprises a plurality of fish rooms, the fish rooms are connected with each other through connecting rods and simple ball hinges to form a chain type structure, the fish room is in a symmetrical double-fan-shaped column structure, ball heads of the simple ball hinges are respectively installed on two end faces of the fish room, ball seats of the simple ball hinges are respectively installed on two ends of the connecting rod, the ball heads can rotate in the ball seats to stretch and fold the fish rooms, the fish rooms are connected with each other to form a chain type overall structure, the fish rooms can be nested and stacked on the bottom of a sea area with a complex structure to form a stable juvenile fish breeding field and a shelter for cephalopods, the juvenile fish breeding field and the shelter can provide a breeding environment for the juvenile fish to feed, stay and avoid enemies, improve the survival rate of the juvenile fish of reef-loving fish, and provide a habitat for cephalopods such as short octopus to avoid enemies.

The utility model relates to the technical field of juvenile fish culture, in particular to a juvenile fish classification screening and feeding integrated device which comprises a screening box and a screening unit, and the screening unit comprises a moving frame, a supporting frame, a bottom plate frame, an air cylinder, a plurality of track plates, a plurality of classification screening plates, a plurality of limiting frames, a feeding assembly and a locking assembly. During actual use, juvenile fishes are placed on the grading screening plate and screened in cooperation with the through holes formed in the grading screening plate from large to small, at the moment, the small juvenile fishes can submerge into the bottom plate frame, and when the juvenile fishes are collected, only an air cylinder needs to be started to make the air cylinder abut against the movable frame to move upwards, and the screened juvenile fishes are driven to be taken out of the screening box as a whole; then the limiting frame is pulled for collection, the locking assembly is used for assisting the limiting frame in resetting, the feeding assembly is used for adding fish feed for feeding, juvenile fishes of different specifications and sizes are temporarily bred in this way, and the growth uniformity of the juvenile fishes is guaranteed.

The invention discloses a microhabitat reconstruction and energy flow regulation and control method for triplophysa orientalis population restoration, and belongs to the technical field of ecological restoration. Aiming at the problems of difficult propagation of natural populations of the triplophysa orientalis and food chain supply interruption caused by limited river habitat bearing capacity and non-uniform seasonal bait supply in an arid region, firstly, an anti-scouring proliferation unit embedded with local plant deadwood is laid in a shallow water slow flow region; constructing a'sheltering-ingestion 'double-effect microhabitat for enabling the oriental triplophysa to avoid natural enemies and to proliferate bait hooked shrimps; secondly, natural artificial proliferation of local wild hooked shrimps is simulated, and directional nutrition cascade regulation and control are implemented according to the energy requirements of three key life history stages, namely recovery after overwintering, gonad development and juvenile fish opening, of the triplophysa orientalis. According to the method, the problem that the survival rate of the triplophysa orientalis fries is low due to insufficient energy in traditional releasing is effectively solved, self-maintenance and propagation expanding of the triplophysa orientalis population are achieved, and then a stable food source guarantee is provided for the geranium wilfordii.

The utility model discloses a kind of accurate feeding devices of juvenile of Pseudogobius lanceolatus, including conveying mechanism, the outer surface of conveying mechanism is provided with limiting piece, the inside of limiting piece is equipped with feeding mechanism, conveying mechanism includes support cylinder, the upper surface of support cylinder is opened with groove, the inner wall of groove is slidably connected with moving block, the inside of groove is inlaid with first bearing;The utility model is rotated by second motor driving rotating lever, rotating lever can drive screw leaf to rotate, make the inside of shell with screw leaf rotation enter the inside of conveying pipe to make its discharging, to be able to ensure the uniformity when feeding, rotate using first motor driving screw rod, moving block will be with the direction of screw rod rotation drive feeding mechanism to move forward and backward, can increase the uniformity of distribution when feeding, can effectively avoid the situation that juvenile is mutually extruded and appears hypoxia when feeding uneven, also can improve the efficiency when feeding feed.

The invention discloses a method for constructing a zebrafish osteoporosis model by using niprednisolone, and aims to solve the problems of high toxicity and poor reversibility of the existing model. The method comprises the following steps: screening 3dpf wild AB strain juvenile fish, culturing to 5dpf, and selecting qualified individuals; in the critical period of vertebra ossification of 5-8 dpf, the niprednisolone is given in a gradient increasing mode; and dyeing with 0.2% calcein at 8dpf, and detecting the vertebra fluorescence area, brightness and quantity to verify the model. Through gradient administration in a critical period, the osteoporosis phenotype is effectively induced, the systemic toxicity is reduced, and the positive drug recovery rate is high. The model is suitable for pathological mechanism research and drug screening, and is standardized in operation, short in experimental period and low in cost.