254 results about "Pelteobagrus" patented technology

The invention relates to a general-purpose high-efficiency bulking compound feed for a culter alburnus and a pelteobagrus fulvidraco. The compound feed is prepared from the following raw materials in portions by weight: 13-35 portions of imported steam fish meal, 10-25 portions of imported direct-heat fish meal, 3-5 portions of maggot powder, 2-8 portions of corn protein powder, 5-10 portions of soybean meal, 2-8 portions of fermented soybean, 2-4 portions of soybean meal, 3-8 portions of cottonseed protein, 2-5 portions of peanut meal, 2-5 portions of colza meal, 3-8 portions of meat / bone meal, 2-5 portions of sleeve-fish visceral meal, 23-33 portions of high protein flour, 1.0-3.0 portions of soya bean lecithin, 2.0-5.0 portions of sea fish oil, 0.4-1.0 portion of choline chloride (50%), 0.1-0.3 portion of vitamin C-phosphate (35%), 0.5-1.5 portions of calcium biphosphate, 0.2-0.5 portion of compound multi-vitamin, 0.5-1.5 portions of composite mineral salt, 0.04-0.2 portion of lutein (20%), 0.1-0.3 portion of phagostimulant, 0.1-0.3 portion of micro-ecological preparation, 0.1-0.3 portion of beta-1,3 glucan, 0.01-0.04 portion of phytase and 0.04-0.08 portion of non-starch polyase. The feed can meet all the nutritional requirements of culturing the culter alburnus and the pelteobagrus fulvidraco, so that fish bodies can grow quickly, high feed conversion rate can be achieved, and the coefficient of the feed ranges from 1.2 to 1.4. By using the feed, the immunity performance and the anti-stimulation capability of the culter alburnus and the pelteobagrus fulvidraco can be obviously enhanced, the energy of the fish bodies can be ensured, and the survival rate is improved by 5-10%.

The invention discloses a swelling granular mixed feed for adult pelteobagrus fulvidraco, relating to the field of pelteobagrus fulvidraco feed. The feed is suitable for feeding the pelteobagrus fulvidraco in adult stage (namely the pelteobagrus fulvidraco in the adult stage with the weight of more than 20g); the feed is a mixture prepared by all raw materials according to the processing method of swelling granular feeds; according to the weight percentage, the feed comprises the following raw materials of: 36-40% of fish meal, 15.58-19.6% of flour, 3.5-4% of cottonseed meal, 16-20% of soybean meal, 6-7% of maize protamine, 4.5-5.5% of fish oil, 6-7% of beer yeast, 1-1.5% of mineral matters, 0.5-0.6% of choline chloride and 0.2% of multivitamin. Compared with normal mixed feed, the swelling granular mixed feed has the advantages of comprehensive nutrition, high livability, little pollution on culture water, greenness, and no public nuisance.

The invention relates to a method for producing male fish, wherein it comprises that: artificially inducing; artificially inducing the female and male fishes; artificial insemination; inverting hormone; feeding seed with Artemia which contains hormone; converting male into biological female; the female grows. The invention comprises sperm inactivation and female diploid generation, to generate ready male fish and normal female fish; crosses the male fish and female fish to obtain the full male fish; inverts the male fish into biological female fish to cross with male fish, to obtain the full super male fish.

An intensive fry breeding system of Pelteobagrus fulvidraco includes a disease prevention and control region for fish body quarantine and sterilization of personnel, tools and water entering the system; a parent fish sorting region including at least one parent fish temporary culture pond for cleaning and specification sorting of parent fishes entering the system from outside; a parent fish spawning inducement region including at least one spawning inducement pond and at least one injection workbench for performing injection spawning inducement to parent fish; an artificial insemination region for completing artificial spawn collection, artificial insemination, artificial debonding and washing; a spawn hatching region including at least one set of fry hatching device for hatching spawn into fry; and a fry temporary culture region including at least one fry temporary culture pond for temporary culture and first-feeding, wherein the disease prevention and control region, the parent fish sorting region, the parent fish spawning inducement region, the artificial insemination region, the spawn hatching region and the fry temporary culture region are all arranged at the production site. The above functional regions are adjacent in sequence. The inventive system has novel facilities, industrialized and large-scale fry breeding and reduced fry propagation cost, and is small in land occupation and low in water consumption.

The invention discloses a kind of pool high product cultivation method for Short head catfish, the main procedure comprise :1 select pool; 2 clean and detoxicate pool ; 3 cultivate natural bait applicator; 4 set out and cultivate oostegopod black shrimp; 5 plant water grass; 6 the setting out and cultivation density; 7 deliver and feed bait applicator; 8 manage water quality;9 prevent and cure disease and harm.

The invention provides a pond eco-culture method for prawns. The method comprises the steps: on the basis of culturing the prawns in a pond, raising at least one kind of aristichthys nobilis, Hypophthalmichthys molitrix, eels, soft-shelled turtles, hemibarbus maculates, pelteobagrus fulvidraco and xenocyprininae; in a culture cycle, adding a composite microbial preparation to a water area of the pond at the interval of 5-30 days according to the ratio of 1-15g / m<3>, wherein the composite microbial preparation contains photosynthetic bacteria, bacillus and nitrobacteria; and meanwhile, arranging a water plowing machine for the culture pond, so as to improve the uniformity and replenishment capacity of dissolved oxygen of water. According to the pond eco-culture method, by an ecological regulating and controlling technology, the reasonable conversion of organic remnant bait and excrement is promoted, and the yield and quality of culture of the prawns are improved.

The invention provides a compound feed of pelteobagrus fulvidraco food fish and a preparation method thereof. The compound feed comprises the following components in percentage by weight: 20-45% of fish meal, 4-10% of meat and bone meal, 15-25% of flour, 4-10% of corn, 10-25% of soy bean meal, 4-10% of rapeseed meal, 1-4% of fish oil, 10-25% of corn gluten meal, 1.0% of multivitamins, 1.0% of multiminerals, 0.1% of immunopotentiator, 0.2% of liver protecting agent, 0.2-0.4% of choline and 1-2% of calcium biphosphate. The compound feed has high quality grinding particle size, mixing uniformity, feed shaping property, water resistance and other characteristics. The pelteobagrus fulvidraco food fish fed with the compound feed has body color close to the natural body color, and the compound feed can improve the anti-virus capability of the pelteobagrus fulvidraco, shorten the breeding cycle, reduce the breeding cost, reduce the sources of the diseases and water body pollution and has obvious economic and environmental benefits.

The invention relates to an artificial propagation method of pelteobagrus fulvdraco, comprising the steps of selecting and rearing parent fish, artificially hastening parturition and hatching fish fry. The artificial hastening parturition comprises the optimized technique of parturition hastening time, setting of fish nest, parturition hastening medicine and dose and secondary injection of medicine; and the fish fry hatching comprises the design of a hatching tank, the optimized hatching technique, and the like. The method has high spawning rate, parturition hastening rate and hatching rate, can save manpower, materials and money, ensures batch production of fish fry in a planed way to meet the requirement of fish fry for different users, ensures that the production and development are unified and further avoids serious ecological damage resulting from killing a large number of male parents.

The invention discloses a method for establishing a full-female pelteobagrus fulvidraco breeding population, which comprises the following steps of: 1) manually hastening parturition and manually inseminating; 2) reversing sex; 3) identifying inheritance sex of the sex reversal pelteobagrus fulvidraco; 4) expanding the population; and 5) establishing the full-female pelteobagrus fulvidraco breeding population, so as to finally establish the full-female pelteobagrus fulvidraco breeding population. According to the invention, an integrating technology of hormone sex reversal combined with pelteobagrus fulvidraco chromosome peculiar molecular markers is adopted, so that XX physiological milter can be confirmed without adopting a test mating method and the breeding time is shortened; compared with the test mating method, one generation can be shortened; the gynogenesis step is omitted, so that the damage to the germ cell genetic material caused by manual induction gynogenesis is reduced; the yield of the XX raun is increased; and the economic and social benefits are obvious.

The invention provides a method for breeding juvenile mussels of hyriopsis schlegeli. The method is characterized by comprising the following steps of cultivation of breeding mussels, larva collection, cultivation of juvenile mussels at a parasitic stage, cultivation of juvenile mussels after juvenile mussel escape and cultivation of small mussels. Preferably, the breeding mussels are 4 to 5 yearsold mussels; the breeding mussels are selected in late autumn, and the selected breeding mussels are cultivated in a special pond; the host fish is pelteobagrus fulvidraco; the host fish is fed withthe mussel meat for nutritional fortification cultivation; the density of glochidia attached to the host fish is 30,000 to 40,000 per kilogram of the host fish; and in the steps of the cultivation ofjuvenile mussels at the parasitic stage, the cultivation of juvenile mussels and cultivation of small mussels, the stocking densities are 1 to 1.5kg / m<2>, 20,000 to 30,000 / m<2> and 150 to 250 / m<2>respectively, and the flow rates are controlled to be 1 to 1.5T / h, 0.5 to 2T / h and 2 to 3T / h respectively. Each day, the nutritional mud is quantitatively added once, and the pond is at least cleanedtwice. By the method, the rate of emergence of the juvenile mussels of the hyriopsis schlegeli is 30,000 to 40,000 mussels / m<2> and improved by 30 percent; and the juvenile mussels bred by the methodhave strong physique and order specifications.

The present invention relates to a method for large-scale cultivating Yellow Catfish and belongs to the technical field of aquiculture. The larval fishes which are hatched for three days are put into the cultivation pond. The cultivate density of larval fishes is 150-200 thousand / mu. The larval fishes are cultivated to the Yellow Catfish with lengths of 2.5-3cm after 20-30 days. The cultivation pond is the earth pond with area of 2mu to 3mu. The thickness of slit on the base of pond is 25-30cm. The pond is placed with sustained-release bag which is filled with fertilizer. The sunny surface of pond back part is provided with a temporary cultivation area of which the area is 1-1.5% of the area of cultivation pond. The bottom adopts the hard material which is nontoxic and smooth at the surface and has the ripple shape. The temporary cultivation area is additionally provided with oxygen heads with the density of 1-2 / square meter. The invention adopts a technique of building the temporary cultivation area for integrating the temporary cultivation stage and larvae cultivating. The method of the invention has the advantages of easy assembling, brief operating program, easy management, and high survival rate of larvae for above 80%. The sustained-releasing technique of fertilizer is adopted for keeping the cleanness of pond base and realizing non-pollution cultivation.

The invention relates to the technical field of aquaculture and particularly relates to a novel feed for juvenile fish of pelteobagrus fulvidraco. The feed is composed of, by mass, 20-40 parts of brown fishmeal, 15-20 parts of flour, 5-10 parts of flour, 15-20 parts of soybean meal, 4-8 parts of peanut bran, 5-10 parts of rapeseed meal, 15-20 parts of corn protein powder, 1-2 parts of soybean oil, 1-3 parts of fish oil, 0.5-1.5 parts of monocalcium phosphate, 0.5-1 part of composite vitamins, 0.5-1 part of composite minerals, 2-3 parts of Chinese herbal medicines, 0.2-0.5 parts of an organic acid phagostimulant, 0.3-0.7 parts of choline chloride, 0.1-0.3 parts of a mildew proofing agent, and 0.2-0.4 parts of an antioxidant. In the invention, Chinese herbal medicines are added to a conventional pelteobagrus fulvidraco feed, so that survival rate of the juvenile fish of pelteobagrus fulvidraco is effectively increased, darkened body color is prevented and bright body color under a wild status of the pelteobagrus fulvidraco can be maintained, thereby improving quality of the juvenile fish. Meanwhile, the Chinese herbal medicines has spectral sterilization effects, so that pathogenic bacteria in water body can be killed effectively, thereby improving situation of water body, increasing the survival rate of the pelteobagrus fulvidraco, reducing application of chemical reagents and reducing breeding cost.

The invention relates to a cultivation method for the summer fingerling of the pelteobagrus. The method comprises the following steps of: (1) selecting a cultivation pond; (2) selecting a water source; (3) preparing the cultivation pond; (4) reasonably stocking; (5) managing the feeding; (6) managing the quality of the water; and (7) preventing and curing the disease and the like. When the method is used for cultivating the summer fingerling of the pelteobagrus, the total artificial forage domestication technologies with different types and different modes are adopted at different times by means of combining with the mode such as eel material slurry sprinkling, dried power sprinkling, bean blocking by powder cluster and the like according to the biologic learnability in different periods to provide a most effective practical measure and method for cultivating the summer fingerling of the pelteobagrus so as to perfect the cultivation technology for the summer fingerling of the pelteobagrus, firstly and successfully break through the key point of the total artificial forage cultivation of the summer fingerling of the pelteobagrus in the domestics, have a theoretical significance and a practice guidance significance for cultivating the artificial forage of the pelteobagrus and improving the forage use ratio, and lay a technical foundation for cultivating the fingerling on a large scale.

The invention discloses a method for quickly establishing an improved breeding system of YY super-male pelteobagrus fulvidraco. The method mainly includes the first step of screening pelteobagrus fulvidraco wild male parents, the second step of carrying out wild parent germplasm resource evaluation on the pelteobagrus fulvidraco, the third step of establishing YY female and YY male basic breeding population through mating with existing YY males, the fourth step of carrying out breeding on the YY female and YY male basic breeding population, the fifth step of carrying out test mating verification on the YY females and the YY males, the sixth step of carrying out quick population expansion on the improved breeding system of the YY female super-male pelteobagrus fulvidraco and establishing the improved breeding system of the YY female super-male pelteobagrus fulvidraco, and the last step of repeating the sixth step, so the improved breeding system can be established. According to the method, genetic diversities of the super-male pelteobagrus fulvidraco are increased, the rate of fertilization, and the incubation rate and the survival rate are improved; meanwhile, the breeding time is shortened, batch breeding can be achieved, the YY female super-male pelteobagrus fulvidraco and XY male fishes with improved characters can be produced in a large-scale mode, the yield can be improved greatly, and economic benefits can be increased.

The invention discloses a modern aquatic product feed suitable for a larav stage of a giant salamander. The feed comprises the following raw materials: 65-75wt% of components A and 25-35wt% of components B, wherein the components A comprise water earthworms, diplopods, freshwater shrimps, shrimps, silkworm chrysalis, chironomus larvas, bean worms, maggots, slugs, moina, loaches, pelteobagrus fulvidraco fries and salangid; and the components B comprise fish meal, fermented peanut meal, soyabean protein powder, snail meat, scallop meat, clam meat, cod-liver oil, vitamins, minerals, sodium alginate, endothelium corneum gigeriae galli, Chinese yam, medicated leaven, poria cocos, dried orange peel, licorice roots, Chinese angelica, honeysuckle and Chinese thorowax. The invention further discloses a preparation method of the modern aquatic product feed suitable for the larva stage of the giant salamander.

The invention provides a cross breeding method for a Pelteobagrus fulvidraco variety namely Huangyou No. 1 with improved growth character. The cross breeding method comprises the following steps: 1, selection of rapidly-growing cross parents: 1.1, selection of a female parent, and 1.2, selection of a male parent; 2, strengthening breeding of parents; 3, artificial parturition hastening; 4, artificial cross fertilization; 5, artificial incubation; and 6, fry breeding. The cross breeding method provided by the invention breeds the first-filial generation of Huangyou No. 1 by cross breeding of female Pelteobagrus fulvidraco and pelteobagrus vachelli; and the first-filial generation of Huangyou No. 1 is tawny in body color, and has extremely significant growth vigor and strong stress resistance.

The invention relates to a fish feed and in particular relates to a compound feed for Pelteobagrus fulvidraco and a preparation method of the compound feed. The compound feed for Pelteobagrus fulvidraco is prepared from the following components in parts by weight: 20-30 parts of fish meal, 8-15 parts of barley, 7-14 parts of rapeseed meal, 5-9 parts of soybean meal, 6-10 parts of honeysuckle flower powder, 3-8 parts of Portulaca oleracea powder, 3-7 parts of licorice powder, 3-7 parts of green bean powder, 3-5 parts of Lycium barbarum powder, 2-6 parts of mulberry powder, 2-6 parts of fish oil, 1-5 parts of tangerine powder, 1-4 parts of shell powder, 1-4 parts of honey, 1-4 parts of olive oil and 1-4 parts of compound vitamin. Pelteobagrus fulvidraco fed by the Pelteobagrus fulvidraco compound feed has low feed coefficient, and has high crude protein content, low crude fat content and high flavor amino acid content in Pelteobagrus fulvidraco muscles.

The invention discloses stratiomyiid meal-containing pelteobagrus fulvidraco feed and an application thereof. The stratiomyiid meal-containing pelteobagrus fulvidraco feed comprises the following components in parts by weight: 24-40 of fish meal, 6-23 parts of stratiomyiid meal, 27 parts of soybean meal, 22-24 parts of high protein flour, 0.5 parts of fish oil, 0.2-3.4 parts of soybean oil, 0.5 part of choline chloride, 0.4 part of vitamin premix, 0.4 part of mineral premix, 1 part of phagostimulant and 1-2.5 parts of microcrystalline cellulose. The stratiomyiid meal-containing pelteobagrus fulvidraco feed has the advantages that the novel pelteobagrus fulvidraco feed is prepared by replacing part of fish meal with the stratiomyiid meal and can effectively supply all nutrient substances required for pelteobagrus fulvidraco by matching of the stratiomyiid meal and other raw materials, so that the survival rate and weight gain rate of the pelteobagrus fulvidraco can be effectively ensured; the nonspecific immune factor serum lysozyme activity, serum and hepatopancreas SOD (Superoxide Dismutase) active content and leukocyte phagocytic capacity of the pelteobagrus fulvidraco are obviously improved, and the immunity of the pelteobagrus fulvidraco is remarkably improved.

The invention discloses a swelling granular mixed feed for parr of pelteobagrus fulvidraco, relating to the field of pelteobagrus fulvidraco feed. The feed is suitable for feeding the pelteobagrus fulvidraco in parr stage (namely the pelteobagrus fulvidraco in the parr stage with the weight of 2-20g); the feed is a mixture prepared by all raw materials according to the processing method of swelling granular feeds; according to the weight percentage, the feed comprises the following raw materials of: 44-48% of fish meal, 15.05-19.55% of flour, 2-5% of cottonseed meal, 15-17% of soybean meal, 3.5-5% of maize protamine, 3.5-4.5% of fish oil, 5-6% of beer yeast, 1-1.2% of mineral matters, 0.5-0.6% of choline chloride and 0.2% of multivitamin. Compared with normal mixed feed, the swelling granular mixed feed has the advantages of comprehensive nutrition, high livability, little pollution on culture water, greenness, and no public nuisance.

The invention relates to a pelteobagrus fulvdraco feed, which is prepared with the following components by weight percentage: 6-10 percent of shrimp meal, 20-40 percent of bran, 10-15 percent of bone meal, 25-35 percent of soybean meal, 5-10 percent of sweet corn gluten meal, 8-10 percent of rice bran powder, 0.4-1 percent of chicken blood powder, 0.1-0.5 percent of salt, 0.5-2 percent of inorganic salt and 0.5-1 percent of mineral additive. The pelteobagrus fulvdraco feed is prepared through the following steps: mixing all the components according to the proportion, crushing the mixture with a multi-purpose fish feed crusher, heating with an annular die type hard particle machine to produce sediment hard-particle feed, and screening the hard-particle feed with a 60-mesh sieve, thereby obtaining the pelteobagrus fulvdraco feed. The weight of each piece of pelteobagrus fulvdraco feed is 0.2g.

The invention provides a compound feed of pelteobagrus fulvidraco juvenile fish and a preparation method thereof. The compound feed comprises the following components in percentage by weight: 25-45% of fish meal, 4-8% of meat and bone meal, 15-20% of flour, 4-10% of corn, 10-20% of soy bean meal, 4-8% of rapeseed meal, 1-4% of fish oil, 10-20% of corn gluten meal, 1.0% of multivitamins, 1.0% of multiminerals, 0.1% of immunopotentiator, 0.2% of liver protecting agent, 0.2-0.4% of choline and 1-2% of calcium biphosphate. The compound feed has high quality grinding particle size, mixing uniformity, feed shaping property, water resistance and other characteristics. The pelteobagrus fulvidraco juvenile fish fed with the compound feed has body color close to the natural body color, and the compound feed can improve the anti-virus capability of the pelteobagrus fulvidraco juvenile fish, shorten the breeding cycle, reduce the breeding cost, reduce the sources of the diseases and water body pollution and has obvious economic and environmental benefits.

The invention discloses a method achieving one-generation breeding of all-female pelteobagrus fulvidraco. The method comprises the following steps that 1, cold treatment induction is conducted on a fertilized ovum obtained by taking super-male pelteobagrus fulvidraco as a male parent and taking ordinary female pelteobagrus fulvidraco as a female parent to obtain all-male triploid pelteobagrus fulvidraco, and oogonial cells are separated from an ovary of ordinary sex-mature female pelteobagrus fulvidraco; 2, the oogonial cells are microinjected to the belly cavities of all-male triploid pelteobagrus fulvidraco larvae; 3, after sexual maturity of the larvae in the second step is achieved, sperms of a donor X chromosome are generated, insemination is conducted between the sperms of the donor X chromosome and the ovum produced by the ordinary female pelteobagrus fulvidraco, and the all-female pelteobagrus fulvidraco can be obtained. According to the method achieving one-generation breeding of the all-female pelteobagrus fulvidraco, the sperms of the X chromosome are directly generated through germ cell transplantation. Therefore, an all-female pelteobagrus fulvidraco breeding system can be rapidly established only through one-generation breeding, and all-female offspring are generated; compared with a traditional all-female pelteobagrus fulvidraco breeding method, the time can be shortened by one year; due to the fact that germ cells of the pelteobagrus fulvidraco are transplanted to the pelteobagrus fulvidraco, transplantation is conducted between the same species and variety, and a higher success rate can be obtained.

The present invention discloses a new-type environmentally protective, nutritional, and high-efficient pelteobagrus fulvidraco compound feed which consists of the following components in weight percentages: 20%-25% of flour, 6%-15% of rapeseed meal, 15%-18% of soybean meal, 5%-8% of meat meal, 10%-15% of imported fish meal, 20%-25% of domestic fish meal, 3% of corn gluten meal, 3% of beer yeast powder, 1%-2% of soy lecithin oil, 2%-3% of soybean oil, 1%-2% of calcium dihydrogen phosphate, 0.2%-0.5% of multivitamin, 1% of compound mineral, 0.1%-0.3% of choline chloride, and 0.02%-0.04% of high temperature resistant phytase, and the sum of the individual component is 100%. The new-type environmentally protective, nutritional, and high-efficient pelteobagrus fulvidraco compound feed in the application of pelteobagrus fulvidraco farming technology can improve survival and growth rates of fish schools, reduce feeding amount, reduce farming costs, and have significant economic value and environmental effects.

The invention relates to pelteobagrus fulvdraco sperm ultralow temperature freezing storage and recovery method. Parent pelteobagrus fulvdraco which is in good shape and good growth, has no disease and is sexually mature is selected, 30Mug / kg LHRH-A2 intraperitoneal injection is carried out to induce sperm increasing, after 36 hours, dissection is carried out to obtain spermary, and the spermary is cut into pieces in a clean mortar cooled in a refrigerator at 4 DEG C in advance, the pieces are diluted by Ringers solution in the proportion of 1:6, balancing is carried out for 30min at 4 DEG C, 10% methyl alcohol is added, and mixing to be uniform is carried out, packaging is carried out, balancing is carried out for 10min 6cm above liquid nitrogen surface, and balancing is carried out for 5min on the liquid nitrogen surface, and then the product is thrown into liquid nitrogen to be stored. Water bath ate 37 DEG C is carried out, activation solution SM is used for activation, pelteobagrus fulvdraco roe is extruded into a container, frozen sperm is added in drops in the sperm roe quantity ratio of 105:1, stirring to be uniform is carried out, then appropriate activation solution SM is added in drops, and stirring to be uniform is carried out, so that fertilization of roe is carried out. The sperm stored by the invention has high quality, has insemination capability and has important theoretical significance and application value on seed quality saving, inheritance breeding, sustainable culture and disease control of pelteobagrus fulvdraco.

The invention discloses an application of black soldier fly larvae to a feed for improving meat quality of aquatic products. Black soldier fly larva powder is added to a feed for aquatic products, andthe black soldier fly larva powder is rich in various nutrient components and functional components, so that the intestine environment of the aquatic animals can be improved, the absorption of the nutrient components in the feed by the aquatic animals can be strengthened, meat quality texture indexes of the muscle elasticity, the adhesiveness, the chewiness, the hardness, the adhesiveness, the cohesion, the recoverability and the like, of the aquatic animals of finless eels, pelteobagrus fulvidraco, japanese sea perches and the like are notably improved, and the application has favorable application prospect and market value.

The invention discloses an artificial compound feed for perca fluviatilis. The artificial compound feed comprises the following components in percentage by weight: 8.0 to 51.5 percent of fish meal, 1.00 percent of fish oil, 1.00 percent of corn oil, 0.24 percent of choline chloride, 1.1 percent of vitamin premix, 1.9 percent of inorganic salt premix, 0.55 percent of calcium dihydrogen phosphate, 0.18 percent of lysine, 0.45 percent of methionine, 8.2 to 15.46 percent of bran, 21.2 to 26.6 percent of wheat middling, 6.01 to 12.82 percent of rapeseed dregs, 0.88 to 2.65 percent of globin powder, 14.87 to 16.79 percent of bean pulp, 10.23 to 12.47 percent of cottonseed pulp and 2.5 to 5.8 percent of yeast; and the components are puffed into a floating granular feed by a conventional method. The invention also discloses a perca fluviatilis feeding method. According to a culture mode of mainly feeding the perca fluviatilis and matching carp, bighead carp, silver carp, tincaeus, grass carp and pelteobagrus fulvidraco Richardson, the perca fluviatilis can be completely domesticated to take the compound feed in 7 to 15 days by adopting the compound feed and a unique intake domesticating method; the feed problem is solved, the water layer and the bait resource of a culture water area are fully utilized, and the growth speed and the yield of the perca fluviatilis are improved; and the compound feed can be used for large-scale culture of the perca fluviatilis.

The invention discloses a compound feed capable of improving stress resistance of pelteobagrus fulvidraco. The compound feed is prepared through mixing the following raw materials and components in parts by weight: 5-10 parts of imported super steam fish meal, 18-35 parts of domestic fish meal, 5-10 parts of domestic meat and bone meal, 5-8 parts of corn protein powder, 20-35 parts of dehulled soybean meal, 18-21 parts of flour, 1.3-1.6 parts of calcium dihydrogen phosphate, 0.13-0.18 part of choline chloride, a stress resistant additive, 1.5-2.5 parts of soybean oil, 1.0-3.0 parts of soybean phospholipid oil, 12.67-18.46 parts of composite vitamins, 10-13.15 parts of composite mineral substances, and 2.5-3.0 parts of zeolite powder. Through the adoption of the compound feed disclosed by the invention, the opportunity that the pelteobagrus fulvidraco resists stress can be reduced, and besides, the stress resistant capacity of the pelteobagrus fulvidraco is improved.

The invention relates to a method for modifying an animal genome by directed mutagenesis, in particular relates to a novel directional transformation method of a specific gene of an animal genome by utilizing zinc finger nuclease. The method comprises the following steps: designing the zinc finger nuclease which specifically identifies and cuts a targeting gene according to a sequence in a target animal target gene region; validating the directional cutting capability and efficiency of the zinc finger nuclease to the target gene by using an independent expression system; and carrying out directional genetic modification on the target gene of the embryo of a target animal to obtain a stable genetic character by utilizing the selected zinc finger nuclease. Furthermore, the invention also relates to a main gene mutation mode which is obtained by using the independent expression system, selecting and utilizing the zinc finger nuclease, and a filial generation obtained through efficient screening and oriented genetic modification by utilizing the selected gene mutation mode. On the other hand, the invention relates to the zinc finger nuclease which is obtained by utilizing the method and is capable of specifically identifying the myostatin gene of pelteobagrus fulvidraco and directionally knocking out the myostatin gene of the pelteobagrus fulvidraco.

The invention discloses a pelteobagrus vachelli growth related SNP molecular marker and application thereof. The transcriptome sequence of a SNP molecular marker source gene is shown as SEQ ID NO:1, from the 5' end, the two SNP loci SNPg.7104G>C and SNPg.7170C>A exist at the 7104th and 7170th sites of the sequence. The SNP loci disclosed by the invention can be used for molecular marker assisted breeding, are not limited by the age, sex and the like of pelteobagrus vachelli, can be used for early breeding of pelteobagrus vachelli, and can significantly promote the breeding process of pelteobagrus vachelli, and the method is accurate and reliable, and is easy to operate.

The invention discloses a method for quickly establishing cross-bred XY holandric pelteobagrus fulvidraco. The method is characterized in that based on mating offspring of pelteobagrus fulvidraco YY physiological female fish and pelteobagrus vachelli XY male fish, cross-bred YY super-male fish is obtained by cultivation through a genetic method so as to mate with common pelteobagrus fulvidraco female fish to produce the XY holandric pelteobagrus fulvidraco. According to the method disclosed by the invention, the breeding period can be obviously shortened; compared with technologies such as Varadaraj and the like, two generations can be shortened; and compared with a method of continuously producing the holandric pelteobagrus fulvidraco by using sex reversal and gynogenesis, one generation can be shortened. Besides, the cross-bred holandric pelteobagrus fulvidraco obtained by the method disclosed by the invention is stable in genetic character, high in growth speed, large, and strong in adaptive capacity to the environment, has cross-breeding advantages and inherits advantages of the holandric fish, so that better breeding and economic benefits can be obtained.