30 results about "Normal female" patented technology

This invention is directed to methods for manipulating sex, reversing sex, changing sex ratio, or moderating crustacean androgenic gland peptide expression in crustaceans for producing a monosex culture. In one embodiment, the present method comprises the step of injecting a dsRNA for the gene of IAG into a male crustacean. In another embodiment, the present method comprises the step of injecting a dsRNA for the gene of IAG into a female crustacean that had acquired a spermatophore (a sperm sac) after mating with a male. In another embodiment, the present invention provides a method of producing a monosex culture of crustacean by introducing a recombinant IAG peptide into the female crustacean, thereby obtains male with altered sexual characteristics for mating with a normal female. In one embodiment, the crustacean is a shrimp or lobster.

Method for producing YY super-male and XY physiological female common carps, with which YY-chromosome super-male common carp can be cultivated and androgenetic YY super-male common carp is produced, where microsatellite markers are used for paternity testing and test crossing confirmation. The YY common carp is crossed with normal female common carp to produce the progenies of only male common carp, and without sex identification, the juvenile male common carp with known sex are subjected to artificial sex reversal to produce XY physiological female common carp.

The invention provides a standard product for polyploid chromosome detection and a preparation method thereof. The standard product comprises a first standard, a second standard and an optional third standard which are respectively positioned inside a separate container, wherein the first standard contains 3.5+/-0.2% of polyploid chromosome gDNA fragments and residual normal female plasma on the basis of total mass (mass) of DNA in the standard; the second standard contains 4-6% of polyploid chromosome gDNA fragments and residual normal female plasma on the basis of total mass (mass) of DNA in the standard; and the first standard contains 8-12% of polyploid chromosome gDNA fragments and normal female plasma on the basis of total mass (mass) of DNA in the standard.

The invention relates to a cultivation method of polyploid pomegranate. The cultivation method comprises following steps: selecting branched flower buds and branches which grow on the top of a diploid plant, inducing the flower to generate polyploid pollen by dropwise adding chemical materials into the flower buds, carrying out hybridizations between induced male flowers and normal female flowers or between induced female flowers and normal male flowers so as to obtain polyploid hybrid fruits, culturing the hybrid seeds in the hybrid fruits so as to obtain polyploid plants, and culturing the polyploid plants in a tissue cultivation mode so as to obtain polyploid pomegranate plants. The cultivation method overcomes the shortage that a large amount of time is consumed by the conventional chromosome reduplication methods. The cultivation method utilizes the fact that the pollen and ovule cells in the reproductive organs are haploids, namely 1n chromosome, during the pomegranate blossom period, uses chemical materials to process the pollen and ovule cells to induce chromosome doubling, thus largely reduces the time of chromosome doubling process, and has the advantages of available materials, simple operation, and high success rate.

The invention discloses an embryo transferring model and a teaching method thereof. According to the proportion of a normal female reproductive system, the lengths, widths, thicknesses, volumes and diameters of all parts (the uterine body cavity, cervix and vagina) of a model are specifically quantitatively designed, combined with the lengths of transferring internal and external catheters, an embryo experimentalist and a clinicist can more intuitively and more accurately understand the relative distance and space of a transferring path, thus the embryo transferring position is better controlled, the clinicist can fully feel certain key difficulties in the transferring path, through repeated practice, the difficulties are overcome in formal transferring operation, and smooth intubation isachieved.

Methods, systems and computer readable media for characterizing quality of CGH arrays, including generating at least one metric for a CGH array that characterizes a property of the CGH array; repeating generation of the at least one metric for at least one additional CGH array; and comparing each metric across all CGH array metric values generated to identify a quality of any one of the CGH arrays characterized, relative to the CGH arrays characterized for that metric. Methods, systems and computer readable media for determining the quality of a CGH array, including calculating a spread of the derivative of log ratio value differences between consecutive probes representing consecutive positions along a chromosome, wherein ratio values are calculated from probe signals from a CGH array, as read by a reader on first and second channels thereof, or from probes signals from two CGH arrays, as read by a single channel reader. Methods, systems and computer readable media for qualifying a CGH array, including quantifying an interarray reproducibility metric characterizing selected sets of probe replicates. Methods, systems and computer readable media for qualifying a CGH array, including generating a Receiver Operating Characteristic (ROC) curve and analyzing false positive and true positive signals from a normal male sample and a normal female sample, as read by a reader on first and second channels thereof, or from one of a normal male sample and a normal female sample on the CGH array and the other of the normal male sample and normal female sample on a second CGH array, as read by a single-channel reader. Methods, systems and computer readable media for determining the quality of a CGH array, including segmenting, by copy number, log ratio signals from probes on the CGH array representing a genome; plotting a probability distribution of the segmented log ratio values; and quantifying separation between copy number peaks resulting from the plotting of the probability distribution.

A method of detecting ovarian cancer in a female test subject comprising determining the amount of plasmenyl-PA or a biomarker having a mass charge ratio of approximately 655.3 in a sample of a bodily fluid taken from the female test subject and comparing the amount of plasmenyl-PA (or the biomarker) in the sample of the bodily fluid taken from the female test subject to a range of amounts of plasmenyl-PA (or the biomarker) found in samples of bodily fluids taken from a group of normal female subjects of the same species as the female test subject and lacking ovarian cancer, whereby a lower amount of the plasmenyl-PA (or the biomarker) in the sample of the bodily fluid taken from the female test subject indicates the presence of ovarian cancer.

The invention discloses an X-chromosome specific molecular marker of Nile tilapia. The nucleotide sequence of the X-chromosome specific molecular marker is as shown in SEQ ID No.3; the X-chromosome specific molecular marker can be commonly used for a plurality of strains of Nile tilapia; the genetic sex determination of the Nile tilapia can be performed economically, rapidly and accurately based on the molecular marker; the molecular marker is advantageous for establishing a method for producing YY supermale fish through mating of supermale fish YY with converted female fish YY; therefore, large-scale production of all male fish fry can be realized through mating of the YY supermale fish and the normal female fish XX; as a result, the yield of culture is improved remarkably, the culture cost is reduced and the economic benefit is enhanced.

The invention belongs to the field of microbial animal cell lines, and in particular relates to a human placental site trophoblastic tumor cell line and a constructing method of the human placental site trophoblastic tumor cell line. Through the in-vitro primary culture on the focus in the uterus of patients suffering from placental site trophoblastic tumor, the human placental site trophoblastic tumor cell line is established; the human placental site trophoblastic tumor cell line is named PSTT-1, and is assigned with the accession number of NO.10882. The human placental site trophoblastic tumor cell line has the biological characteristics that the cells grow in single layer in a wall-attaching manner, the contact inhibition is eliminated, the state is nonuniform, and the cells are in the star-shaped or fusiform-shaped flat structure with multiple bulges; the growth in in-vitro culture is good, and the stable and continuous passage for more than 30 generations can be realized; the karyotype analysis result is the normal female karyotype: 46, XX. The PSTT-1 cell line can be used for the research on the nosetiology, the transfer mechanism, the drug-resistant mechanism and the like of the placental site trophoblastic tumor diseases.

The invention relates to specific molecular markers of sexes of silurus meridionalis and a silurus meridionalis genetic sex identification method and a unisexual fish production method based on the molecular markers. The invention screens specific molecular markers of X and Y chromosomes of silurus meridionalis for the first time. A silurus meridionalis genetic sex PCR identification method established based on the molecular markers has the characteristics of high efficiency, accuracy and stability, can be applied to wild silurus meridionalis in laboratories and different watersheds, and can economically, quickly and accurately distinguish normal female fishes, normal male fishes, transformed female fishes, transformed male fishes and super-male fishes, thereby realizing large-scale production of complete female fish fries, significantly increasing the breeding yield, reducing the breeding cost, and improving economic benefits. Furthermore, a large number of unisexual fish fries can beobtained so as to be applied to fundamental research.

The invention relates to an orthotic device for female legs and knees. The orthotic device is characterized by comprising a body, wherein the body is fixedly connected with a magnet sheet; the upper end of the magnet sheet is fixedly connected with a magnetic interlayer, and the lower end of the magnet sheet is fixedly connected with a demagnetization layer; a long waist-shaped hole is formed in the middle part of the demagnetization layer; the two sides of the body are provided with telescopic sections; the body is provided with four fixed belts which are uniformly and symmetrically distributed on the two sides of the body; each fixed belt is provided with a bonding plate A; the upper end of the demagnetization layer and the lower end of the demagnetization layer are provided with bonding plates B. The orthotic device has the function of correcting leg gestures for the female with handicapped legs, and also enables legs of normal female users to keep line of beauty all the time, so that the body shape is more attractive, and the orthotic device is quick and convenient to use and has higher market value.

The invention relates to a voyeur which is convenient for fixing a crowd suction tube, which comprises a voyeur body, and a suction tube fixing device is arranged on one side of the voyeur body, and the suction tube fixing device includes a mounting rod, a connecting sleeve, a fixing clip and a limit rod. One end of the rod is fixed on the peeping body, the connecting sleeve is set on the installation rod, the connecting sleeve is connected with the installation shaft through the connecting rod, the fixed clip is composed of a fixed half clip and a movable half clip, and one end of the movable half clip is set on the installation shaft , the other end of the movable half clip has a positioning through hole, the limit rod is fixed on the fixed half clip, and the positioning through hole of the movable half clip passes through the limit rod and is fixed on the limit rod. The present invention is on the basis of the original ordinary peeping, and adds the artificial flow suction tube fixing device, which is convenient for fixing the artificial flow suction tube. It is not polluted, safe and labor-saving, and has good promotion and application value.

The new method selects first-filial generation and the breed with less contour difference to the female parent as parent of cross breeding, and plants the two lines of hybrid in the space-time with normal female parent fructification to raise the uniformity of first-filial generation and utilize the vigor of second-filial generation.

The invention relates to the technical field of traditional Chinese herbal medicines, in particular to a traditional Chinese medicine for treating external genital coldness, and aims to overcome defects of the prior art and solve the technical problem of providing a traditional Chinese medicine for treating external genital coldness. To solve the technical scheme, the invention adopts the technical scheme that the traditional Chinese medicine for treating external genital coldness contains the following traditional Chinese herbal medicines in parts by mass: 10-15 parts of processed rehmannia, 10-15 parts of pulp of dogwood fruits, 10-15 parts of Chinese yams, 10-15 parts of radix ophiopogonis, 10-15 parts of schisandra chinensis, 10-15 parts of angelica sinensis, 5-10 parts of codonopsis pilosula, 10-15 parts of photiniae folia and 5-8 parts of dried ginger. The traditional Chinese medicine is used for treating external genital coldness, activity and secretion of ovaries can be recovered, normal oestrogen secretion is recovered, a normal female hormone level is recovered, and a normal sexual life is recovered.

The invention discloses a method for testing the acoustical communication capability of rana nigromaculata. The method comprises the following steps: collecting rana nigromaculata from a natural water body; setting treatment groups and control groups, and performing natural matching treatment on rana nigromaculata; putting all rana nigromaculata in a glass aquarium in a dark room to adapt for 5-15 minutes; recording songs of normal female and male rana nigromaculata in the mating season in advance; playing normal songs of rana nigromaculata, observing response of rana nigromaculata of different treatment groups, and recording corresponding songs and data; and acquiring song parameters, and analyzing experimental data. By adopting the method, important indication significance is provided to further understand the relationship between songs and behaviors of animals and efficiently reflect the body situations and living environment situations of the animals.

The invention discloses a multi-interface SFP network communication connector and a production process thereof. The connector includes a fixed disk and a rotation disk which is rotatable with an innerring of the fixed disk, wherein periphery of the fixed disk is equipped with multiple annularly-distributed female terminals, and multiple multi-interface type male terminals which can be rotatably switched with the female terminals are mounted around the rotation disk. The connector is advantaged in that in the use process, when one female terminal or male terminal is damaged, switching to the next normal female terminal and male terminal can be performed, the multi-interface type mode is equivalent to a spare mode, when other terminals are damaged, switching rotation is performed to constantly ensure that signal transmission is always stable.

The invention discloses a device for effectively controlling insect outbreak, and in particular relates to a method that a bionic material and a micro mechanical design principle are utilized, an insect sex pheromone induction and collection principle is fully utilized, an artificial mechanical female insect attracts a male inset, and the two insets are mated. After the male inset feeds aedeagus into the genital cavity of the female insect, partial aedeagus of the male insect is removed by a mechanical cutting-off means, so that the male insect does not have the mating capacity but still has mating behavior; the male insects which cannot normally mate, and normal female insects perform mating behavior, and only parthenogenesis offspring can be generated. The whole design can effectively control the insect outbreak, and also can effectively control the extinction of the kind of living thing (the parthenogenesis offspring are reserved).