45 results about "Rana nigromaculata" patented technology

The invention discloses a building method for a frog and rice co-culture pond. The building method comprises the steps of A, the whole pond, wherein according to the size, the frog and rice pond is designed into a standard pond, and digging is conducted by using an excavator; B, a three-stage single ditch, wherein digging is longitudinally conducted in the middle of the frog and rice pond; C, a feeding table area, wherein the width of the feeding table area in the frog and rice pond is set as 1.5 meters, a reflective thin film is laid on all the feeding table area, and the periphery of the reflective thin film is pressed down and covered with soil; D, a rice field area, wherein the other portions, except for the portions in which the three-stage single ditch and the feeding table area arelocated, of the frog and rice pond are provided with the rice field area; E, water inlet and outlet pipes, wherein all the water inlet and outlet facility pipes are U-shaped pipes; F, an escape-proofnet, wherein the periphery of the frog and rice pond is covered with bamboo poles, and rana nigromaculata is prevented from jumping out of the pond; G, an anti-bird net, wherein one end of the escape-proof net is fixed to the outer periphery of the frog and rice pond, the middle of the escape-proof net is supported by using reinforcing steel bars, and birds are prevented from damaging rice and frogs. The method is easy to implement, the operation is simple, the ecological benefits are effectively improved, feeding management is facilitated, and the economic efficiency is improved. The problemsare solved that in an existing pond, water drainage is not smooth, the yield of the rana nigromaculata is low and the benefit is low.

A method for cultivating black-spotted frogs, comprising the steps of: (1) selecting species of frogs; (2) cultivating species of frogs; (3) laying eggs and fertilization; (4) hatching of frog eggs; (5) feeding and management of tadpoles (6) the management of young frogs; (7) the management of becoming frogs; the present invention's egg laying capacity is big, and hatching rate is high, effectively avoids the ovum softening of frog ovum, and ovum collapses flatly, avoids the ovum rupture of frog ovum, hatches The hatching rate is above 90%, and the hatching quality is high; the survival rate of tadpoles is high, and the survival rate of tadpoles is above 95%; the black-spotted frog has strong vitality, large size, good quality, improved color and taste, and increased survival rate. The output is greatly improved, the growth cycle is shortened, the breeding cost is reduced, and the economic benefit is improved.

Provided is a rana nigromaculata spawn hatching method. The method comprises the steps that after rana nigromaculata lays zygotes in a spawning pond for 2 h to 3 h, zygote masses are collected; the zygote masses are put into a net cage to be divided into zygote granules, the zygote granules are quickly transferred into a hatching pond to be hatched, the embryo development condition is observed during hatching, dead zygotes are removed in time, and when the zygotes are about to be hatched into tadpoles, micro-flowing water is kept in the hatching pond or water is replaced. The method is simple, convenient to operate, high in operability, easy to master and high in hatchability which is at 90% or above, hatching quality, tadpole survival rate which is at 95% or above and water quality; the phenomena that spawn membranes of rana nigromaculata spawns are softened, ova are flat, the spawn membranes of the rana nigromaculata spawns are broken, and the spawn masses are omitted are effectively avoided, and torrential rain and sun exposure are avoided; hatching synchronization of the tadpoles can be effectively guaranteed, and the phenomenon that growth of the tadpoles is affected due to the fact that the sizes of the tadpoles are not uniform is avoided.

The invention relates to a rana nigromaculata antimicrobial peptide as well as a gene and application thereof, and belongs to the technical field of biomedicine. The rana nigromaculata antimicrobial peptide is a gene coded single-chain polypeptide separated from an amphibian rana nigromaculata of China, wherein the molecular weight is 1,647.02 Daltons, and the isoelectric point is 11.05; and the complete sequence of the rana nigromaculata antimicrobial peptide is phenylalanine-isoleucine-proline-leucine-valine-serine-glycine-leucine-phenylalanine-serine-arginine-leucine-leucine-glycine-lysine. The coded gene of the rana nigromaculata antimicrobial peptide consists of 316 nucleotides, wherein the mature coded part of the coded gene contains 142nd-186th nucleotides. The artificially synthesized rana nigromaculata antimicrobial peptide has a strong antibacterial effect, has the advantages of high safety, simple sequence and convenience in synthesis, and can be used as a novel anti-infective agent for application.

The first objective of the invention is to provide a feed additive for rana nigromaculata. The feed additive for the rana nigromaculata is prepared from the following raw materials: antioxidant Chinese herbal medicine ingredients, astaxanthin and organic rare earth; and the organic rare earth is a compound of lanthanum and organic acid. The feed additive disclosed by the invention is capable of enhancing immune function, anti-stress capacity and antioxidant capacity of the rana nigromaculata, as well as promoting metabolism and improving nutrient utilization capability; moreover, the feed additive is capable of solving the problem of bluing of the skin color of the rana nigromaculata so as to retain natural state of the skin color of the rana nigromaculata, so that the economic values of the rana nigromaculata are increased. In addition, the raw materials of the feed additive disclosed by the invention basically leave no residues in the rana nigromaculata bodies, and cause no influence on meat texture of the rana nigromaculata. The second objective of the invention is to provide a preparation method of the feed additive for the rana nigromaculata. The preparation method comprises the following steps: crushing Chinese herbal medicines, and carrying out mixing; adding a NaCl solution; and then, carrying out grinding, drying, crushing and sieving. The preparation method is simple in steps; and the prepared feed additive is good in effects.

The invention provides a rana nigromaculata static predation feeding domestication and frog-rice coexistence cultivation method. The method comprises the following steps of 1, laying bait and a mesh on the upper layer of a rice field ditch; 2, selecting young rana nigromaculata; 3, conducting young rana nigromaculata cultivation; 4, adopting a purifying, rejuvenating, selecting and breeding methodand 5, training static predation. The rana nigromaculata static predation feeding domestication and frog rice coexistence cultivation method prevents a frog habitat from being directly polluted, is beneficial to the healthy growth of frogs and increases the rice planting area, the rana nigromaculata habitat area and the rana nigromaculata putting quantity; a purifying, rejuvenating, selecting andbreeding method is adopted, and in the propagation period every time, and by selecting good rana nigromaculata and through long-term (3-5 generations) domestication, the purpose of improving physiological habits of rana nigromaculata can be achieved.

The invention relates to a culture method of rana nigromaculata. The method comprises the following steps of 1, rana nigromaculata breeding: adult rana nigromaculata breeds polliwog in a breeding pool, and wheat flour is fed to the polliwog; 2, rana nigromaculata culture: after the polliwog grows into the rana nigromaculata, the rana nigromaculata is transferred into a culture pool to be cultured, and compound feed for batrachia starts to be fed; 3, rana nigromaculata hibernation: during the hibernation, the rana nigromaculata is transferred into a hibernation pool, before the transferring, straws are paved on the bottom surface of the hibernation pool, and then, soil is covered on the straws; 4, daily management: the water is changed once every one to two days, and the water changing quantity in each time is 1/4 to 1/3 of the total water quantity in the culture pool. The rana nigromaculata cultured through the method has high growth rate, high spawning rate, high survival rate and strong vitality.

The invention provides a planting and breeding integrated system for rice, rana nigromaculata and mushroom. The system comprises a main aisle penetrating through the middle of rice field, a fence assembly is arranged at the edge of the rice field, a plurality of breeding units are arranged on the two sides of the main aisle, accessory aisles are reserved between the adjacent breeding units, and the accessory aisles are connected with the main aisle. One end of the rice field is provided with a water storage pond, the two sides of the main aisle are each provided with a water supply tank, a feeding pavilion is arranged in the center of the water storage pond, froglet breeding assemblies are movably arranged in the portions, around the feeding pavilion, of the water storage pond, and frogletfeeding assemblies are arranged at the edges of the feeding pavilion. After the technical scheme is adopted, the economic benefit of the rice field is greatly improved, the quality of agricultural products is improved, the ecological environment of the rice field is improved, and the planting and breeding integrated system is a new modern ecological cycle agricultural development mode which has the functions of stabilizing grain production, promoting fishing, increasing the efficiency, improving the quality and achieving environmental friendliness.

The invention belongs to the technical field of frog breeding, and particularly relates to a breeding method for increasing the condition factor of froglets of rana nigromaculata. The method comprisesthe following specific steps that (1) the depth of a breeding pond is established to be 72-78 cm at the beginning of March, potamogeton malaianus is evenly planted at the bottom of the pond, the froglets of rana nigromaculata are put into the breeding pond at the beginning of April, and light-filling illumination treatment is conducted by using green light every day; (2) frog feed is put four times every day, and the feeding amount every time is same, wherein the frog feed is prepared from sorghum rice, small glutinous millet, grape skin residues, yellow mealworm, pig livers, barley leaves, cactus, pine pollen, steviol glycoside, Chinese bushcherry seed extract, mulberry polysaccharide and bacillus natto; an excellent living environment is provided for the growth of the froglets of rana nigromaculata, the capabilities of absorbing and digesting nutrients and resisting harmful bacteria are improved, the robust growth of the frog bodies is effectively promoted, the frogs are fat, big and strong, the muscle content is high, and the conversion synthesis and accumulation of protein, iron and selenium can also be promoted.

The invention discloses a fish-frog-rice symbiotic culture method in a pond. According to the method, rana nigromaculata and fishes are cultured in the pond, and rice is planted. A 'fish-frog-rice symbiosis' method is adopted, the fishes can catch and feed on aquatic animals and plants, injurious insects dropping into water from rice and residual feeds of the frogs; nutrition is provided for the rice by utilizing excrements of the fishes and frogs, an effect of purifying water in the pond is achieved, and the injurious insects on rice stems and leaves are eaten by the frogs, so three-dimensional symbiosis is realized among the fishes, frogs and rice in the pond. The method has the effects of purifying the water and killing insects, and high yield, high efficiency and pollution avoidance can be realized. By virtue of the fish-frog-rice green ecological high-efficiency symbiosis technology, zero release of pond water can be achieved, and green and pollution-free ecological food materialsare provided for the society.

The invention discloses a rana nigromaculata jump force measuring device, which comprises a weight having a certain weight, a transparent box with height marks, a rope and a bottom plate. The bottom plate is provided with a horizontal slide groove; the weight having a certain weight is arranged at the left end of the horizontal slide groove; a rana nigromaculata, the weight of which is measured, is placed in the transparent box with height marks; the rana nigromaculata, the weight of which is measured, is connected with the weight having a certain weight through the rope; and the transparent box with height marks is arranged at the right end of the horizontal slide groove. The device is simple in structure and convenient to operate and can measure the jump force of the rana nigromaculata quickly.

The invention relates to compound feed for rana nigromaculata. The compound feed is prepared from the following raw materials in parts by weight: 15 to 20 parts of white fish meal, 1 to 3 parts of spray-dried animal blood cells, 4 to 8 parts of chicken meal powder, 10 to 15 parts of soycomil, 3 to 5 parts of pupa peptide protein powder, 10 to 20 parts of soybean meal, 10 to 15 parts of hulled barley, 8 to 12 parts of high protein flour, 2 to 3 parts of yeast extract, 5 to 8 parts of corn, 1 to 2 parts of soyabean lecithin oil, 2 to 3 parts of soybean oil, 1 to 2 parts of monocalcium phosphate,and 3 to 4 parts of core additive. The invention also relates to a preparation method of the compound feed for the rana nigromaculata. The compound feed provided by the invention is prepared according to the growth requirement of the rana nigromaculata, and is balanced in nutrition, suitable for the growth of the rana nigromaculata, good in palatability, and capable of maintaining the natural andcolorful body color of the rana nigromaculata to be the same as the appearance of wild rana nigromaculata.

The invention relates to a method for inducing rana nigromaculata to eat artificial formula feed. The method comprises the following technical steps (1) when 70% or above of tadpoles of the rana nigromaculata complete metamorphosis, four limbs of the tadpoles grow up, and the tadpoles start to go ashore, plastic feed tables are placed at four corners of a culture pond, 3-5 feed tables are placed at each corner, and the artificial formula feed is placed on the feed tables; (2) froglets of the rana nigromaculata which complete metamorphosis and go ashore are driven to the feed tables at four corners of the culture pond by using bamboo poles every day, the situation is kept for 15-30 minutes, driving is conducted five times every day, then one time is reduced every three days, driving is conducted twice every day after ten days, and driving is conducted totally for 15-20 days. The particle size of the artificial feed is smaller than or equal to 0.8 millimeter; waste feed needs to be removed and replaced by the fresh artificial formula feed before the froglets are driven each time; after inducing is successfully completed, the feed is timely added to the feed tables according to the growth condition of the rana nigromaculata until the feed is fully distributed on the periphery of the culture pond. According to the method, the problem can be effectively solved that the rana nigromaculata eats the artificial formula feed difficultly, the investment is reduced, the breeding cost is lowered, and the breeding economic benefit is improved.

The invention discloses a fish-frog-vegetable symbiotic culture method in a pond. According to the method, rana nigromaculata and fishes are cultured in the pond, and vegetables are planted. A 'fish-frog-vegetable symbiosis' method is adopted, the fishes can catch and feed on aquatic animals and plants, injurious insects dropping into water from vegetables and residual feeds of the rana nigromaculata; nutrition is provided for the vegetables by utilizing excrements of the fishes and frogs, an effect of purifying water in the pond is achieved, and the injurious insects on vegetable leaves are eaten by the rana nigromaculata, so three-dimensional symbiosis is realized among the fishes, frogs and vegetables in the pond. The method has the effects of purifying the water and killing insects, and high yield, high efficiency and pollution avoidance can be realized. By virtue of the fish-frog-vegetable green ecological high-efficiency symbiosis technology, zero release of pond water can be achieved, and green and pollution-free ecological food materials are provided for the society.

The invention relates to the technical field of frog breeding, in particular to a method and device for breeding rana nigromaculata in a rice field in a segmented purse seine manner. Small breeding areas are formed by arranging segmented purse seines; when rana nigromaculata froglets are thrown, the rana nigromaculata froglets are thrown into the small breeding areas; the rana nigromaculata froglets have relatively small sizes, and on the basis of the same number of the put rana nigromaculata, the culture space required by the rana nigromaculata froglets is relatively small, so that the swimming space of the rana nigromaculata froglets is reduced, the swimming distance of the rana nigromaculata froglets can ensure the memory of the rana nigromaculata froglets on feeding tables; the froglets can return to the feeding tables in time to find food, so that the mortality rate of froglets of rana nigromaculata died due to hunger is greatly reduced; and the putting survival rate of the rana nigromaculata froglets is increased, the supplementing amount of the froglets is reduced, the breeding cost is reduced, and the economic benefits are improved.

The invention discloses a rana nigromaculata culture method which includes the steps: site selection; rana nigromaculata pond building; rana nigromaculata selecting and mating; tadpole feeding management; young rana nigromaculata feeding management; adult rana nigromaculata feeding management; disease prevention and treatment. According to the method, later generations reproduced by rana nigromaculata are fed by artificial compound feed in the whole process when the movable baits are completely eliminated, the culture yield of the rana nigromaculata is greatly improved, the rana nigromaculatais scientifically cultured from aspects such as culture pond building, rana nigromaculata selecting and mating, tadpole feeding management, young rana nigromaculata feeding management, adult rana nigromaculata feeding management and disease prevention and treatment, the survival rate of the rana nigromaculata from incubating to maturing can be high, high-density culture can be implemented, and heavy, fat, delicious, edible and medicinal rana nigromaculata is produced.

The invention discloses an artificial feeding method of frogs, which comprises the following steps of: (1) constructing a field, (2) putting up a shed frame, (3) arranging a cofferdam, (4) raising baby frogs, (5) culturing tadpoles, (6) putting the tadpoles in a pond, (7) disinfecting aquatic weed, (8) injecting water into the culture pond and controlling a water level, and (9) after tadpole incubation, obliquely immerging the edge of a basin with the tadpoles under a water surface in a shallow area with the aquatic weed to slowly release the tadpoles. According to the method, in an adopted frog-rice mixed culture mode, insecticide is not required to kill pests for planted rice; the produced rice is green and nuisance-free; the pests growing in a rice planting process provides an enough food source for culture of rana nigromaculata; the production cost is lowered; and economical, ecological and social benefits are improved.

A domestication method of a full-feed black-spotted frog species. In the first year, the eggs of the black-spotted frog are cultivated in a simulated wild environment and fed with live bait; in the second year, after the young frogs of the black-spotted frog complete metamorphosis and land , feed with live bait for one month, after that, gradually reduce the amount of live bait, add feed to the live bait for feeding, and gradually increase the proportion of feed until all feed is used for feeding; in the third year, in the black After the young frogs of spotted frogs complete their metamorphosis and come ashore, they are directly fed with feed, and the young frogs will have adverse reactions. After feeding with feed for one month, some young frogs are eliminated, and some young frogs are used to feeding with feed; in the fourth year, Black-spotted frogs are raised in the frog pond and fed with feed. The invention subverts the tradition, reduces the production cost, liberates a large amount of labor force, is suitable for large-scale industrial production, greatly shortens the growth cycle, greatly improves the output, the yield per mu reaches more than 3,000 jin, and the economic benefit is significantly increased.

The invention discloses a rana nigromaculata and rice symbiotic planting and breeding method, which comprises the following steps: S1, rice field selection, region division and transformation, S2, parent selection and breeding, and S3, adult frog fishing and rice harvesting, and also discloses a specific management method of a breeding pond, a hatching pond, a nursery pond and a breeding pond, which comprises the steps of regulating and controlling the area proportion of each region, and managing the breeding pond, the hatching pond, the nursery pond and the breeding pond. The feeding proportion and density of parents, egg masses, tadpoles and froglets in the breeding pond, the hatching pond, the nursery pond and the culture pond are strictly regulated and controlled, the water level and water quality are strictly regulated and controlled according to climate characteristics in different periods, micro-running water equipment is erected in the hatching pond to improve the water quality, increase the dissolved oxygen and the like, the rana nigromaculata culture density can be effectively controlled, the rice field space is efficiently utilized, and the yield of rana nigromaculata is increased. According to the method, the rana nigromaculata can be bred in advance to form ordered breeding and controllable production, so that the rana nigromaculata can come into the market in advance, high-quality and large-specification rana nigromaculata can be bred, the specification of 80% of commercial rana nigromaculata can reach 40 g or above, and therefore the method has higher economic benefits.

The invention discloses a breeding method of rana nigromaculata. The breeding method comprises the following steps of 1, parent frog selection; 2, parent frog breeding; 4, frogspawn hatching; 5, tadpole breeding management; 6, young frog management; 7, adult frog management. According to the breeding method of rana nigromaculata, the frogspawn yield is large, the hatching rate is high, egg membrane softening and egg flattening of frogspawn are effectively avoided, egg membranes of the frogspawn are prevented from being broken, the hatching rate is 90% or above, the hatching quality is high, the survival rate is high, and the tadpole survival rate, the young frog survival rate and the adult frog survival rate are all 95% or above. The rana nigromaculata is strong in vitality, large in sizeand good in quality, the color and the taste are improved, the yield of the rana nigromaculata per mu is greatly improved, the growth period is shortened, the breeding cost is reduced, and the economic benefit is improved.

The invention discloses a rana nigromaculata meat processing device. The rana nigromaculata meat processing device comprises a machine base and a rotating roller arranged on the machine base, whereinthe rotating roller is provided with an opening in one side, a feeding pipe which is used for transporting rana nigromaculata meat is arranged inwards in the opening, and an outlet of the feeding pipefaces the downward rotation side of the rotating roller; sliding grooves are uniformly formed in the outer wall of the rotating roller in a spaced manner; a moving plate is arranged in each sliding groove; a plate outlet allowing each moving plate to pass is formed in a position corresponding to the corresponding moving plate, on the inner wall of the rotating roller; and a rolling roller is arranged above the bottom in the rotating roller. According to the rana nigromaculata meat processing device disclosed by the invention, steamed rana nigromaculata meat can be continuously subjected to drying and stir-frying processing; in the overturning and rolling process, the steamed rana nigromaculata meat can be quickly dried and stir-fried; and besides, the moving plates which can be automatically expanded and contracted are used for cooperation for overturning and stirring, the whole process is efficient and intelligent, and the operation is simple, convenient and reliable.

The invention discloses a breeding method for increasing the survival rate of rana nigromaculata. The breeding method comprises the following steps of 1, selection of breeding rana nigromaculata; 2, breeding of the breeding rana nigromaculata; 3, oviposition and fertilization; 4, incubation of rana nigromaculata eggs; 5, breeding management of tadpoles; 6, management of young rana nigromaculata; 7, management of adult rana nigromaculata. The method has the advantages that the oviposition amount is large, the incubation rate is high, egg membrane softening of the rana nigromaculata eggs is effectively avoided, the eggs are prevented from being flat, egg membranes of the rana nigromaculata eggs are prevented from being broken, the incubation rate is 90% or above, the incubation quality is high, the survival rate is high, and the survival rates of the tadpoles, the young rana nigromaculata and the adult rana nigromaculata are all 98% or above. Rana nigromaculata is high in vitality, largein size and good in quality, the color and the taste are improved, the yield of the rana nigromaculata each mu is greatly improved, the growth period is shortened, the breeding cost is reduced, and the economic benefit is improved.