The invention discloses an extraction optimization method of
cactus exosomes, which comprises the following steps: selecting
cactus pomace, and carrying out vacuum freeze-
drying treatment to obtain a freeze-dried
cactus pomace raw material; performing
reflux extraction twice; combining the two supernates, concentrating under reduced pressure, and
drying under reduced pressure to obtain a water extract; adding an
ethanol solution as an extraction
solvent to obtain an
alcohol extraction sample; performing first-stage centrifugal
impurity removal, second-stage centrifugal
impurity removal, third-stage centrifugal
impurity removal, fourth-stage centrifugal enrichment, refining purification and storage to obtain a cactus
exosome sample; and performing sample injection detection. The extraction concentration and purity of the cactus exosomes are improved through a pretreatment-multistage
centrifugation combined method. The detection result shows that the concentration of the
exosome in the water extract reaches 4.3 * 10 parts / ml, the concentration of the
exosome in the
alcohol extract reaches 9.2 * 10 parts / ml, and the concentration of the exosome in the water extract and the concentration of the exosome in the
alcohol extract are obviously higher than those in puree and
fruit juice. The method is clear in parameter, high in extraction efficiency and suitable for large-scale preparation of the cactus exosome.