The invention discloses
vinasse alcohol-soluble
polysaccharide as well as a preparation method and application thereof, and belongs to the technical field of
polysaccharide extraction. In order to improve the
alcohol solubility and
oxidation resistance of
vinasse polysaccharides, the invention provides a
vinasse alcohol-soluble
polysaccharide and a preparation method and application thereof, and the preparation method comprises the following steps: mixing vinasse and water, crushing and centrifuging, taking supernate, firstly adding
amylase for enzymolysis, then adding
neutral protease and
cellulase for enzymolysis, performing
enzyme deactivation on the obtained vinasse enzymatic
hydrolysate, and concentrating to obtain the vinasse alcohol-soluble polysaccharide. The method comprises the following steps: dissolving vinasse in
distilled water, dialyzing and desalting, enabling the total
ethanol concentration of the
system to reach 38-53% Vol, then carrying out alcohol
precipitation and
centrifugation, concentrating the obtained alcohol
precipitation supernatant, intercepting by adopting an
ultrafiltration membrane to obtain 4000-6000 Da trapped fluid, and freeze-
drying to obtain the vinasse alcohol-soluble polysaccharide. By optimizing the enzymolysis technology and controlling the alcohol
precipitation condition and the target molecular weight of the polysaccharide, the activity, purity and alcohol
solubility of the polysaccharide are remarkably improved, and the polysaccharide has good
antioxidant activity and in-vitro anti-
liver injury activity.