227 results about "Elicitor" patented technology

The invention provides a method for improving the content and aromas of tobacco secondary metabolite products. During any tobacco plant growth period or after tobacco harvesting, a leaf surface spraying method, a stem top spraying method or a root-irrigation method is adopted to implement one or more of salicylic acid elicitors and / or jasmonic elicitors on the tobacco plants or the tobaccos so as to induce synthesis of the tobacco secondary metabolite products. The method can not only remarkably increase the content of tobacco essential oil, petroleum ether extract and glandular hair secreta and the like and improve tobacco aroma quality, but also improve yield of some important natural products in the tobacco such as solenesol and provide high-content raw materials for extraction of the natural products. Meanwhile, the method can activate tobacco plant immune system and improve insect resistance, disease resistance and stress resistance of the tobacco plants.

A liquid micronutirent elicitor involving a cell to cell signal transduction system within a plant which is applied to propagules (seed and / or plant) causes natural defensive responses to be produced by the seed and / or plant. This substance may exist as a seed coating, irrigation water, and / or foliar spray for a period of time so that the propagules may have enhanced disease control until the propagule develops sufficiently to fend for itself against parasitic nematodes. The benefits of incorporating the solution include increased vigor, blooms, and harvests. Crops include legumes including soybeans, as well as wheat, canola, corn, peanuts, sunflowers, peppers, tomatoes, grapes, and potatoes.

The invention provides a fermentation method for high cordyceps militaris biomass and high cordycepin content, which belongs to the technical field of bio-engineering. A cordyceps militaris strain is activated, inoculated to a first stage seed tank and further expanded to a fermentation tank, when a certain biomass is achieved in the fermentation tank, a biotic elicitor of Xylaria type endophytic fungus from binkgo biloba extract liquid and non-biotic elicitors of sodium acetate and ammonium sulfate are added for promoting the production of cordycepin in bacteria. The fermentation method adopts an optimized culture medium for effectively increasing the mycelial growth of cordyceps militaris; furthermore, under the regulation role of the elicitors, the cordyceps militaris biomass and the cordycepin content in the bacteria are respectively increased by 1.76 times and 7.14 times compared with the contrast culture medium without the addition of the elicitors and respectively increased by 2.35 times and 23.25 times compared with the ordinary improved PDA. The fermentation method greatly improves the production amount of cordyceps militaris mycelia and the content of the active characteristic ingredient, thereby having broad market prospect.

The invention discloses a phellinus igniarius mycelium liquid fermentation method by utilizing a fungal elicitor for improving the yield of flavone of phellinus igniarius, which comprises the steps of: inoculating a fungal elicitor strain for activated culture, and then inoculating and culturing a fungal block to obtain a fungal elicitor mycelium through filtration; then drying, grinding and crushing the fungal elicitor mycelium, adding the crushed fungal elicitor mycelium into ethanol for soaking, filtering the obtained solution, collecting filter residue, orderly adding a mixed solution of chloroform and normal butanol, acetone and water into the filter residue for washing, adding trifluoroacetic acid into a dry substance after the air-drying, performing boiling water bath for 0.5 to 2 hours, then filtering the obtained solution, taking a filtrate for neutralization, standing the filtrate, using a microporous filtering film with the pore diameter between 0.4 and 5mu m to filter the filtrate for sterilization to obtain the fungal elicitor, and cryopreserving the fungal elicitor at the temperature of between 10 and 20 DEG C below zero; inoculating a phellinus igniarius strain for activated culture, and then inoculating a fungal block for fermentation culture; and adding the fungal elicitor into the phellinus igniarius to continue culturing to obtain a phellinus igniarius mycelium within 1 to 5 days of liquid fermentation culture of the phellinus igniarius. The method has simple process, low cost and remarkable effect, can greatly improve the content of the flavone of phellinus igniarius cells, and is a production method with industrialization prospect.

The invention relates to an application method of an elicitor in the hairy root of American ginseng, belonging to the field of crop biotechnology. The process comprises the following steps: (1) induction of the hairy root of American ginseng; (2) measurement of the growth dynamic and ginsenoside content of the asexual system of the hairy root of American ginseng; (3) optimization of culture conditions for the hairy root of American ginseng; (4) preparation of an elicitor; (5) regulation of enzyme activity in a metabolic pathway. In the method provided by the invention, the combination of the culture conditions for the hairy root of American ginseng is put forward, the influence of two elicitors, namely salicylic acid and methyl jasmonate, on the metabolism of American ginseng is studied in order to increase the saponin output, a basis is provided for large-scale production of American ginseng saponin, and a foundation is laid for realizing industrial production of American ginseng saponin so as to meet the ever-increasing needs of people.

Isolated fragments of an Erwinia hypersensitive response elicitor protein or polypeptide that elicit a hypersensitive response in plants and isolated DNA molecules that encode those fragments are disclosed. Isolated fragments of hypersensitive response elicitor proteins or polypeptides, which elicit a hypersensitive response, and the isolated DNA molecules that encode them can be used to impart disease resistance to plants, to enhance plant growth, and / or to control insects on plants, either by applying the hypersensitive response eliciting fragments to plants or plant seeds or by providing transgenic plants or plant seeds transformed with a DNA molecule encoding a hypersensitive response eliciting fragment.

The invention provides a preparation method of triptolide and ramification and composition of the triptolide. After an elicitor plays a role in tripterygium suspended cell sap, a high yield of triptolide and ramification of the triptolide can be gotten. The extractive gotten by the method has a good effect on inhibiting tumors, a hepatitis b virus and a human immunodeficiency virus (HIV); the extractive can be prepared into soft capsules, or hard capsules, or dropping pills or troches, or dispersible tablets, or granules, or injection, or oral liquid, or suppository which are applied in oral or skin or mucous membrane or rectal way, by that the extractive is used for being solely or being matched with other drugs or adding auxiliary materials which can be acceptable in pharmacy.

The invention relates to a soil conditioner as well as a preparation method and application of the soil conditioner. The soil conditioner comprises the components based on parts by weight: 10-15 parts of biochemical fulvic acid, 10-20 parts of polyacrylamide, 5-10 parts of burdock oligosaccharide, 5-10 parts of chitosan, 1-5 parts of zinc sulfate, 1-2 parts of boric acid and 50-70 parts of trichoderma pseudokoningii fermentation liquor. The invention also provides the preparation method and the application of the soil conditioner. According to the invention, biochemical fulvic acid and the polyacrylamide are combined, and trace elements zinc and boron, the disease-resistant elicitor burdock oligosaccharide, chitosan and the biological control fungi trichoderma pseudokoningii which are necessary to plants and deficient in the soil are loaded, so that the soil conditioner having the functions of improving the physicochemical property of the soil, inhibiting the soil-borne diseases, promoting the growth of the plants and inducing the resistance of botanical system is prepared, and is environment-friendly.

The present invention discloses a method using Agrobacterium rhizogenes (Ar) gene to transform Rhodiola sachalinensis A.Bor to achieve a hairy root system for Salidroside production. By preparation of explant and cultivation in Ar bacteria liquid, Rhodiola sachalinensis A.Bor is induced to grow hairy roots by genetic transformation; the PCR testing proves that hairy roots are generated by transformation; a fermenter is used for massive production of hairy roots, and precursor substances and elicitors are added to improve the Salidroside content. The method is applied to produce Salidroside to substitute the drying-up Rhodiola sachalinensis A.Bor resources.

The invention relates to a Ganoderma lucidum mycelium liquid fermentation method for improving ganoderic acid content by utilizing Fungal Elicitors. The method comprises the steps of the preparation of the Fungal Elicitors, the liquid fermentation culture of the Ganoderma lucidum and the co-culture of the Ganoderma lucidum and the Fungal Elicitors. The method has the advantages of simple process, low cost, obvious effect, high content of the ganoderic acid, and being a manufacturing method with huge industrial prospect.

The invention discloses an orange biological preserving agent based on yeast, sodium bicarbonate and elicitor activity, composed of cryptococcus laurentii suspension, rhodotorula glutinis suspension, rhodosporidium toruloides suspension, sodium bicarbonate, auxin, salicylic acid and water. The preserving agent has 1*1010-1*1012 cryptococcus laurentii cells, 1*1010-1*1012 rhodotorula glutinis cells, 1*1010-1*1012 rhodosporidium toruloides cells, 5-50 grams of sodium bicarbonate, 10-400mg of auxin, 1-100mg of salicylic acid and the reminder consisting of water per liter of the preserving agent. The accession number of cryptococcus laurentii is CGMCC No.3590, the accession number of rhodosporidium toruloides is IMI 394084, and the accession number of rhodotorula glutinis is CGMCC No.3589. The orange biological preserving agent disclosed in the invention can control orange postharvest diseases without using chemical bactericides.

The invention discloses effector protein Avh87 derived from phytophthora cinnamomi, and a coding gene and application thereof. The protein has the amino acid sequence of SEQ ID No. 1 in the sequence table. The nucleotide sequence expressing the protein is represented by SEQ ID No. 2 in the sequence table. Experiments show that the gene can inhibit plant cell hypersensitive reactions induced by apoptotic precursor protein Bax, or inhibit plant cell death induced by pathogenic phytophthora elicitors INF1. The effector protein Avh87 is of great significance in enriching molecular mechanism information of the interaction between the phytopathogenic oomycetes and hosts, and in establishing a comprehensive prevention and control technology strategy for phytopathogenic oomycete diseases.

The invention provides a preparation method for celastrol derivatives, products and application thereof. The preparation method takes celastrol a microbial metabolic drug, and enables microorganisms to metabolize celastrol through a special elicitor, so that a plurality of high yield celastrol derivatives based on celastrol's hydroxyl, reduction or carboxylation and other substitution modification can be obtained at the same time. Being green and environment friendly, the method provided by the invention has strong selectivity and high yield, and can make large-scale use of microorganisms to prepare celastrol derivatives. The prepared celastrol derivatives have the characteristics of high efficiency and low toxicity, etc., and can practically be used for treatment of tumors, viral diseases and nerve injury diseases, thus providing broad application prospects for celastrol derivatives.

Disclosed are peptides that induce an active plant response, but not a hypersensitive response, when applied to plant tissue. These peptides also preferably exhibit improved solubility, stability, resistance to chemical degradation, or a combination of these properties. Use of these peptides or fusion polypeptides, or DNA constructs encoding the same, for modulating plant biochemical signaling, imparting disease resistance to plants, enhancing plant growth, imparting tolerance to biotic stress, imparting tolerance and resistance to abiotic stress, imparting desiccation resistance to cuttings removed from ornamental plants, imparting post-harvest disease or post-harvest desiccation resistance to a fruit or vegetable, or enhancing the longevity of fruit or vegetable ripeness are also disclosed.

The invention discloses a tangerine biological antistaling agent based on the activity of rhodosporidium and a fruit elicitor, which consists of rhodosporidium suspension, gibberellin / kinetin, salicylic acid, auxin and water, wherein each 1 liter of antistaling agent contains 1*1010 to 1*1012 rhodosporidium cells, 50 to 150 milligrams of gibberellin or kinetin, 8 to 100 milligrams of salicylic acid, 10 to 500 milligrams of auxin and the balance of water; and the rhodosporidium is rhodosporidium paludiagenum Fell and Tallman of which the collection number is IMI 394084. The biological antistaling agent can control post-harvest diseases of tangerine fruits effectively on the premise that chemical bactericides are not used.

The invention discloses the application of burdock fructo-oligosaccharide as disease resistance elicitor in agriculture. Burdock fructo-oligosaccharide is natural oligosaccharide with good water solubility and stability extracted form burdock root. The invention comprises applying burdock fructo-oligosaccharide as disease resistance elicitor to promote plant growth and induce plant disease resistance. Burdock fructo-oligosaccharide can promote plant growth in different seedling stages, reinforce procatarxis of the seedlings, and promote the formation of strong seedlings.

The invention relates to penicillium protein elicitor EPTP and application thereof in improving plant disease resistance. The protein sequence of the EPTP protein elicitor is shown as SEQ ID NO.2, and the molecular weight is 19247 Da. EPTP induces significant increase of activity of phenylalanine ammonialyase, peroxidase and polyphenol oxidase, and when the elicitor induces tobacco anaphylactic reaction, an anaphylactic reaction marker gene hin1 is induced to express; it is indicated through semiquantitative RT-PCR results that EPTP can induce tobacco to obtain systemic acquired resistance, cause up-regulation of the tobacco blade resistance gene transcription level, and induce expression of resistance gene related to the salicylic acid path and the jasmonic acid path. After being applied, EPTP can improve resistance to viruses and pathogenic bacteria by plants, takes effects efficiently, does not pollute environment, reduces dosage of chemical pesticide and has wide application prospects as a plant immunity activator.

The liquid elicitor of chitin and chitosan and micronutrient trace elements of the present invention involves induced systemic resistance (ISR) / innate immunity responses within plants. Dilute solutions are applied to propagules (fractions of microgram per seed and / or plant), which causes natural biotic defense responses by seedlings and / or plants. The application may be as seed coating, irrigation water, and / or foliar spray, wherein propagules bioactivate systemic disease defenses against bacteria, fungi, insects and parasitic nematodes. Benefits include enhanced biocontrol of seed viability, seed germination, seedling vigor, plant growth, flowering and harvest yields for legumes, grains, potatoes, vegetables, fruits, trees, sugar beet, and grass. By nature, this homeopathic invention benefits the entire environment by being natural, biodegradable, and by promoting beneficial soil organisms. This new class of agricultural chemistry will ameliorate adverse effects of toxic pesticides through use of the present invention in integrated pest management (IPM) programs.

The invention discloses a rooting-promoting, disease-resistant and plant-strengthening agent for cotton and a water, fertilizer and pesticide integrated application method. The rooting-promoting, disease-resistant and plant-strengthening agent is mainly prepared from a growth regulator, a microelement, a signal molecule donor and an elicitor capable of improving the disease resistance of the cotton, wherein the growth regulator is prepared from indolebutyric acid, sodium nitrophenolate, naphthylacetic acid and diethyl aminoethyl hexanoate through compounding, the microelement is prepared from zinc sulfate, ferrous sulfate, ammonium molybdate, amino acid powder and ammonium hydrogen phosphate through compounding, the signal molecule donor is NO donor sodium nitroprusside, and the elicitor is a penicillium dry mycelium extracting solution. The problems that a drip irrigation technology does no good to root development and severe diseases are caused by successive cropping to the cotton are solved, the growth regulator accelerates growth of root systems, the physiological function time of the root systems is prolonged, the disease-resistant elicitor enhances the resistance of cotton plants, growth of lateral roots is further promoted in combination of the signal molecule donor, and the disease-resistant response of the cotton plant is promoted. The rooting-promoting, disease-resistant and plant-strengthening agent can promote growth of roots and resist diseases in multiple angles; the agent can be injected into irrigation water through a fertilizer pump, integrated application of water, fertilizer and pesticides in the drip irrigation cotton field of the northwest inland is realized, and the agent can promote growth of roots, resist diseases and strengthen seedlings and has remarkable disease resisting and yield increasing effects.

Disclosed are peptides that induce an active plant response, but not a hypersensitive response, when applied to plant tissue. These peptides also preferably exhibit improved solubility, stability, resistance to chemical degradation, or a combination of these properties. Use of these peptides or fusion polypeptides, or DNA constructs encoding the same, for modulating plant biochemical signaling, imparting disease resistance to plants, enhancing plant growth, imparting tolerance to biotic stress, imparting tolerance and resistance to abiotic stress, imparting desiccation resistance to cuttings removed from ornamental plants, imparting post-harvest disease or post-harvest desiccation resistance to a fruit or vegetable, or enhancing the longevity of fruit or vegetable ripeness are also disclosed.

The invention discloses a callus culture method of snow lotus herb, comprising the steps of selection and asepsis of plants, induction and culture of callus and large-scale culture. The core technology of the method is selection of the following large-scale culture medium: MS+0-50g / L of cane sugar+0-10g / L of agar+0.1-5.0mg / L of alpha-naphthylacetic acid+0.1-5.0mg / L of 6-benzyladenine. During large-scale culture, an abiotic elicitor, 40-60mu mol / L of SNP+40-60mu mol / L of MJ+40-60mu mol / L of SA is added. The method is easy to operate, and the active ingredient is high in content and low in production cost, does not pollute environment and can reach the level of industrialization. Syringin produced by the culture technology in the invention is invariant, high in content, low in cost and short in cycle and is very competitive in the markets.

Disclosed are hypersensitive-response eliciting peptides that exhibit improved solubility, stability, resistance to chemical degradation, or a combination of these properties. Use of these peptides or fusion polypeptides, or DNA constructs encoding the same, for modulating plant biochemical signaling, imparting disease resistance to plants, enhancing plant growth, imparting tolerance to biotic stress, imparting tolerance and resistance to abiotic stress, imparting desiccation resistance to cuttings removed from ornamental plants, imparting post-harvest disease or post-harvest desiccation resistance to a fruit or vegetable, or enhancing the longevity of fruit or vegetable ripeness are also disclosed.

The invention relates to a plant cell culture technology, in particular to a method for biosynthesizing abietane diterpenoid compounds by using cephalotaxus fortune culture cells. The method comprises the following steps of: placing cephalotaxus fortune cells in a common culture medium, and culturing, wherein50 to 150g of the cells are inoculated in each liter of wet weight of the culture medium; and adding an inducer at the final concentration of 10 to 2,000 mu M and 50 to 200g of adsorbent into each liter of culture medium at the initial stage of cell exponential growth, wherein the inducer is an abiological inducer or fungal elicitor. The method is a biosynthesis method and is stable and environment-friendly; and products are completely adsorbed onto the adsorbent, so that the operation process of separation and purification is greatly simplified, the cost is reduced and industrial production is easy to realize. Meanwhile, seven abietane diterpenoid compounds comprising a new compound are synthesized. The abietane diterpenoid compounds have high bioactivity, and are important lead compounds, and the technology has important commercial application prospect.

The invention relates to a bacillus subtilis protein elicitor AMEP412. The amino acid sequence of the bacillus subtilis protein elicitor is shown as SEQ ID NO.1. The invention also relates to the newfunction of the bacillus subtilis protein elicitor AMEP412. The invention discloses protein eliciting functions of a protein AMEP412 with an unknown function, including plant growth promotion, improvement of disease, salt and alkali, drought and low temperature resistance ability of plants, and new materials are accumulated for biological prevention and control work.

The invention discloses a method of utilizing taxus lateral root proliferation to produce paclitaxel, the steps are that: (1) annual or biennial taxus branches are selected as cutting materials; (2) after the cuttings are sequentially injected into KMnO4 solution, sucrose solution, and plant growth hormone solution for soaking, the cuttings are conveyed to cutting substrate; (3) the cuttings are pulled out after the roots grow up, and are continually conveyed to the cutting substrate after being processed with hormone; (4) a certain quantity of the lateral roots are cut for producing the paclitaxel; (5) being dried under temperature control at the temperature that is less than or equal to 55 DEG C, pure products of the paclitaxel are obtained after processes of grinding, concentrated organic solvent extraction, column chromatography, solvent recovery, crystallizing and drying; or the cut taxus lateral roots are cultured in a improved Murashige and amp;Skoog fluid nutrient medium, bacteriostat is added in the nutrient medium, 2 to 9 days after fresh taxus lateral roots are induced and processed by elicitors, the pure products of the paclitaxel are extracted. The invention provides a new approach for medicine sources of the paclitaxel, and the shortcomings in artificial cultivation of the taxus are solved of low rooting rate, low survival rate after the roots are cut, slow roots proliferation speed, etc.

The invention relates to functional oligosaccharide having the growth promoting function of disease-resistant elicitor in cultivation of plant seedlings, in particular to application of chitosan oligosaccharide as the disease-resistant elicitor to cultivation of the plant seedlings. Chitosan oligosaccharide has the function of promoting seed germination, can enhance quality of the seedlings and promotes formation of strong seedlings. By measuring the content of malondialdehyde related to disease resistance, it is proved that chitosan oligosaccharide is an excellent disease-resistant inducing factor and can be prepared into chitosan oligosaccharide biopesticide to be used. Chitosan oligosaccharide serving as the main component can be combined with other components to be prepared into non-polluted pesticide, and further chitosan oligosaccharide derivative products are developed.

The invention discloses a method for hydroponic breeding of huperzine serrate and induced improvement to huperzine a of the huperzine serrate. According to the method, wild naturalized huperzine-serrate branches with stem tips are used as cuttings, and rapid hydroponic breeding of the huperzine serrate is achieved in a nutrient solution containing hormones and precursor substances with a certain ratio; compared with conventional soil cutting, the method realizes that rooting time is short, the breeding coefficient is large, and the rooting percentage is as high as 98%. The precursor substances enable the huperzine a content of the hydroponic huperzine serrate to be increased by 70% compared with wild plants; the leaf surfaces or / and the roots of the plants are sprinkled with elicitors in a certain concentration, so that a secondary metabolism system of the huperzine serrate is regulated, the huperzine a content of the hydroponic huperzine serrate is increased 1.3-1.6 times, the yield of the huperzine a is increased, and the situation of shortage of huperzine-serrate resources is alleviated to a certain degree. Besides, the method achieves low production cost and a short cultivation period, is not restricted by the natural environment and climate, and has broad application prospects and high popularization value.

The invention discloses a verticillium dahlia secreting type protein elicitor VdPEL1 and application thereof. The protein can improve the plant disease resistance and can be used as an inductor for inducing the plant immunity and can be applied to the field of plant disease prevention and treatment. The amino acid sequence of the protein is shown as SEQ ID NO:2. The engineering strain expression is used for obtaining high small-to-big protein, so that the disease resistance of tobaccos and cotton can be improved; the related plant diseases include tobacco mosaic, gray mold and cotton verticillium wilt. The protein elicitor can obviously improve the plant disease resistance; the effect is fast; the action time is long. The VdPEL1 provides a new path for improving the plant disease resistance.

Disclosed are peptides that induce an active plant response, but not a hypersensitive response, when applied to plant tissue. These peptides also preferably exhibit improved solubility, stability, resistance to chemical degradation, or a combination of these properties. Use of these peptides or fusion polypeptides, or DNA constructs encoding the same, for modulating plant biochemical signaling, imparting disease resistance to plants, enhancing plant growth, imparting tolerance to biotic stress, imparting tolerance and resistance to abiotic stress, imparting desiccation resistance to cuttings removed from ornamental plants, imparting post-harvest disease or post-harvest desiccation resistance to a fruit or vegetable, or enhancing the longevity of fruit or vegetable ripeness are also disclosed.