71 results about "Elicitor" patented technology

The invention discloses a plant immune induced resistance protein PpAE4 and application thereof, and the protein can induce plant resistance and can be used as a plant immune activating factor to be applied to the field of prevention and treatment of plant diseases. The amino acid sequence of the protein is as shown in SEQ ID NO: 2. High-concentration protein can be obtained through expression of the engineering strain, the disease resistance of plants to soybean rust germs can be improved when the protein is used for treating the plants, and the protein is low in needed concentration, quick in effect taking and long in acting time. The PpAE4 provides a new way for improving plant disease resistance and preventing and treating plant diseases.

The present application relates to the technical field of elicitor peptide MsPep and its application, and discloses the use of elicitor peptide MsPep in improving the genetic transformation efficiency of alfalfa, wherein the application takes the leaf of alfalfa as an explant, introduces an exogenous gene through Agrobacterium tumefaciens, and adds the elicitor peptide MsPep in each stage of genetic transformation, so as to significantly improve the dedifferentiation rate, the out-growth rate, the differentiation rate, the out-bud rate, the rooting rate and the transformation efficiency, and shorten the transformation period, and the present application has application prospect in improving the genetic transformation of alfalfa and other plants.

The invention belongs to the field of microbiology and molecular biology, and particularly relates to a method for extracting myxococcus flavus dormant mucospore RNA (Ribonucleic Acid). The method specifically comprises the following steps: taking a certain amount of myxococcus flavus liquid, and inducing sporocarp development by using a common starvation induction method; scraping the formed sporocarp structure, resuspending with sterile water, and destroying the sporocarp structure and mucospore coating by using a certain proportion of organic reagent; centrifuging, taking a water phase, adding an acetate buffer solution with a certain concentration, and precipitating with isopropanol to obtain nucleic acid substances; and removing DNA by using a conventional kit to obtain RNA. Aiming at the characteristic that the mucospore RNA is difficult to extract, the invention develops a simple, convenient and efficient method for extracting the mucospore RNA, and provides an effective way for deeply researching the ecological characteristics of the myxobacteria.

The application provides a polypeptide, a plant immune elicitor and application thereof. The polypeptide has 10 or 11 amino acid residues; the amino acid at the first position of the polypeptide is serine, and the amino acids at the 9th and 10th positions are both cysteine; if the polypeptide has 11 amino acid residues, the amino acid at the 11th position of the polypeptide is asparagine. The polypeptide can solve the problem that there is no small peptide secreted by gramineae plants in the prior art, and is suitable for the field of plant immune elicitors.

The invention discloses a broad-spectrum plant-source disease-resistant excitant for rice blast and a preparation method thereof, and belongs to the technical field of rice blast prevention and control, in the broad-spectrum plant-source disease-resistant excitant, potassium glutamate serves as amino acid salt, not only provides a nitrogen source for plants, but also participates in the metabolic process in the plants and enhances the stress resistance of the plants. S-allyl-L-cysteine is a sulfur-containing amino acid derivative, has antioxidant and disease-resistant activity, and can stimulate a defense mechanism of plants and resist infection of magnaporthe oryzae. 2-piperidinecarboxylic acid has the effect of inhibiting growth of pathogenic bacteria and can directly interfere with cell wall synthesis of magnaporthe oryzae, so that diseases are relieved. Chitosan as a biological polysaccharide can induce plants to generate resistant substances, enhance the strength of plant cell walls, form a physical barrier and prevent invasion of pathogenic bacteria. Salicylic acid is an important signal molecule in a plant body and can activate systematic resistance of the plant and improve the overall disease resistance.

The application discloses a plant immunity elicitor protein PpAE4 and application thereof, the protein can induce plant resistance, and can be applied to the field of plant disease prevention and treatment as a plant immunity activator. The amino acid sequence of the protein is shown as SEQ ID NO:2. High-concentration protein can be obtained by using an engineering strain for expression, and the protein can be used for treating plants to improve the disease resistance of the plants to soybean rust fungi, and the required concentration is low, the effect is quick, and the action time is long. The PpAE4 provides a new way for improving the disease resistance of plants and preventing and treating plant diseases.

The invention relates to the technical field of agricultural biology, in particular to cotton bollworm salivary protein Ha22, a recombinant vector, recombinant bacteria and application of the cotton bollworm salivary protein Ha22. The invention provides a cotton bollworm salivary protein Ha22. The amino acid sequence of the cotton bollworm salivary protein Ha22 is as shown in SED ID NO. 1. Transient expression of the Ha22 protein on tobacco can cause tobacco cell necrosis, stable overexpression of the Ha22 protein on cotton plants can induce cotton defensive gene response and positively regulate plant insect resistance, and the Ha22 protein has great application value for cotton insect-resistant breeding. In addition, the protein has the active characteristic of an elicitor, so that the purpose of green prevention and control can be achieved by spraying whole protein to plants through in-vitro expression and inducing plant resistance.

The invention discloses application of pseudopenicillin variotii in preparation of a resistance inducer for preventing and treating plant diseases, and belongs to the technical field of plant protection. The invention provides an application of Panax variotii in prevention and treatment of plant diseases, the application concentration of the Panax variotii is 0.1 microgram / mL-1 microgram / mL, a plant resistance inducer containing the Panax variotii can be sprayed on plant leaves or fruit surfaces and has a remarkable prevention effect on anthrax leaf blight, and the higher the concentration is, the better the prevention effect is; the pseudopenicillin variotii can promote synthesis or activity improvement of catalase, peroxidase, polyphenol oxidase, glutathione S-transferase and superoxide dismutase in apple bodies, and also can remarkably induce up-regulation expression of disease-resistant related genes such as MdPR2 and MdPR5, so that the disease resistance, the antioxidant level and the plant immunity of apples are enhanced; a novel and efficient plant resistance inducer application scheme is provided for apple disease control and physiological regulation.

The invention discloses a novel vegetable health magnesium resistance inducer as well as a preparation method and application thereof, the main components of the vegetable health magnesium resistance inducer are trimethylglycine (GB) and magnesium oxide nanoparticles (MgONPs), and the components have the characteristics of no toxicity, harmlessness, no pollution and the like. The invention finds that the'vegetable health magnesium 'resistance inducer can activate plant hormone signals, enhance plant immunity and resist virus diseases. The composition can be sprayed to vegetable crops before the high-incidence period of vegetable virus diseases to prevent and treat virus diseases, is simple and convenient, has strong operability, and can effectively improve the broad-spectrum resistance of plants to various vegetable virus diseases. Compared with traditional pesticides, the composition is more green and safer, meets the requirements of sustainable and healthy development of agriculture, and has huge application potential.

The application discloses application of an immune elicitor protein FvXynC and a protein combination in plant disease resistance, and belongs to the technical field of plant immune elicitors. A new immune elicitor protein FvXynC is found from F. pseudograminearum, and the immune elicitor protein has the core functions of activating plant immune response and improving plant resistance to F. pseudograminearum, can be used as an efficient plant immune inducer, provides a new technical path for plant disease resistance improvement and green plant disease control, and has important agricultural application value. The FvXynC and the immune elicitor protein FvGH43A are compounded, so that the resistance level of corn to F. pseudograminearum can be synergistically improved, and the problem that the plant disease control effect of a single immune elicitor protein is limited is solved.

This invention relates to the field of plant disease biological control technology, and particularly to a Fusarium oxysporum cell wall extract, its preparation method, and its application. The extract is derived from the polysaccharide fraction of the cell wall of Fusarium oxysporum spores or hyphae, and contains at least one pathogen-associated molecular pattern (PAMP) component. This component is a non-protein, thermally stable, species-nonspecific elicitor capable of forming an aqueous dispersion system. The extract of this invention can be recognized by plant PRRs and activate PTI immunity. Rehmannia glutinosa tubers treated with the extract showed a significantly increased germination rate after inoculation with Fusarium oxysporum. It also induces superoxide anion production, upregulates the activities of superoxide dismutase, peroxidase, and catalase, and enriches differentially expressed genes in the MAPK signaling pathway, jasmonic acid metabolism pathway, and phenylpropane biosynthesis pathway. Furthermore, the extract can directly inhibit Fusarium oxysporum colony expansion, conidia production, and mycelial growth. Extracts derived from spores or hyphae show no significant difference in inducing disease resistance, and the raw materials are flexible and easy to industrialize. This extract is derived from the cell wall of the pathogen itself, making it environmentally friendly and free from the risk of chemical pesticide residues. It is suitable for the green control of root rot disease in Rehmannia glutinosa.

The invention belongs to the technical field of biological prevention and control, and provides application of N-palmitoyl aspartic acid in improvement of resistance of rice bacterial blight, which is characterized in that N-palmitoyl aspartic acid is prepared into a resistance inducer for prevention and control of bacterial blight pathogen infection in a three-leaf one-core period of rice. The inhibition effect of the N-palmitoyl aspartic acid on the Xanthomonas oryzae pv. Oryzae is evaluated, and the result shows that the N-palmitoyl aspartic acid effectively reduces the gene expression of the Xanthomonas oryzae pv. Oryzae, inhibits the growth and propagation of the Xanthomonas oryzae pv. Oryzae, improves the resistance of rice, can replace or reduce the use of chemical bactericides, and is environment-friendly.

The invention relates to a preservative as well as a preparation method and application thereof. The preservative comprises a bacillus velezensis microbial inoculum and an elicitor cooperating with the microbial inoculum, and the addition amount of the elicitor is 0.01-0.1% of the total mass of the preservative. The preservative further comprises at least one of a protective agent, a preservative and an antioxidant; the bacillus velezensis microbial inoculum comprises the following components in parts by mass: 20-80 parts of a bacillus velezensis microbial inoculum, 0.001-0.1 part of an elicitor, 1-25 parts of a protective agent, 0.05-1 part of a preservative and 0.05-1 part of an antioxidant. The effective viable count in the bacillus velezensis microbial inoculum is greater than or equal to 10 < 9 > CFU / mL; the bacillus velezensis microbial inoculum comprises at least one of bacillus velezensis strains LTS-AF1, LTS-AF2, LTS-AF3 and LTS-AF4, and the bacillus velezensis microbial inoculum comprises at least one of bacillus velezensis strains LTS-AF1, LTS-AF2, the strain action effect is improved, the action time is prolonged, and the strain activity is stabilized.

The present invention relates to a method for producing steviol glycosides of Stevia rebaudiana, which method comprises the following steps: preparing the Stevia rebaudiana nodal explants having two internodes; propagating the explants in temporary immersion systems (SETIS) with controlled immersion cycles; adding an elicitor after 21 days of culture; and transferring to a liquid culture medium enriched with growth regulators and nutrients that are essential to plant growth and development.

The invention provides an application of sclerotinia sclerotiorum small peptide Xyn25 in improvement of plant disease resistance, and belongs to the technical field of biopesticides. The invention provides a sclerotinia sclerotiorum small peptide Xyn25. The amino acid sequence of the sclerotinia sclerotiorum small peptide Xyn25 is shown as SEQ ID NO: 1. The sclerotinia sclerotiorum small peptide Xyn25 can efficiently activate plant immune response and improve the disease resistance of plants. The embodiment of the invention shows that the sclerotinia sclerotiorum small peptide Xyn25 can improve the resistance of wheat to gibberellic disease and rice to banded sclerotial blight. On the basis, the invention further provides an immune resistance inducer which comprises the sclerotinia sclerotiorum small peptide Xyn25 and auxiliary materials. The immune resistance inducer provided by the invention meets the development requirements of pesticide reduction, quality improvement and efficiency improvement in green agriculture, and has a good application prospect in the aspects of reducing the dependency degree of chemical pesticides and the like.

The invention discloses a plant immune induced resistance protein Ps131903 and application thereof. The amino acid sequence of the immune induced resistance protein Ps131903 is shown as SEQ ID NO.2. The protein Ps131903 can reduce the harm of phytophthora sojae and pythium ultimum to soybeans, reduce the harm of phytophthora capsici to chilies and tomatoes and reduce the harm of pyricularia grisea to rice. The compound has the advantages of low use concentration, quick effect and broad spectrum, and provides a new way for improving the plant resistance, thereby having wide application prospects in agricultural production.

The present application relates to the field of genetic engineering, in particular to an immune elicitor GH20 gene and the application of the encoded protein thereof in the prevention and treatment of corn large spot disease. The nucleotide sequence of the immune elicitor GH20 gene is shown as SEQ ID NO. 1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO. 2. The present application first discloses the biological function of the immune elicitor GH20 gene and the encoded protein thereof, and further verifies that the immune elicitor GH20 gene and the encoded protein thereof have significant prevention and treatment effects on corn large spot disease through prokaryotic expression, and can be widely applied in the biological prevention and treatment of corn large spot disease. It can be seen that the present application provides a new biological preparation for the prevention and treatment of corn large spot disease, and lays a good foundation for the creation and development of new biological pesticides.

Described are plant defense elicitors or plant elicitor compositions and their use in, for example, agricultural applications, more particularly, to protect plants against pests or pathogens. This includes the corresponding methods of and uses in the protection of plants and crops by application of these plant defense elicitors or plant elicitor compositions. The active ingredient(s) of the plant defense elicitor or plant elicitor comprise lignin-derived phenolic oligomers with a degree of polymerization (DP) of 2 to 8 (preferably 2-4) (such as lignin-derived dimeric (diphenolic), lignin-derived trimeric compounds (triphenolic) and lignin-derived tetrameric compounds) and the engineered or synthesized structurally similar compounds. More particularly, described is a plant defense elicitor wherein an active ingredient of the plant defense elicitor comprises depolymerized lignin containing as active ingredient lignin-derived phenolic oligomers (such as lignin-derived dimeric compounds (diphenolic), lignin-derived trimeric compounds (triphenolic), and lignin-derived tetrameric compounds) and engineered or synthesized structurally similar compounds.

This invention discloses a green control method and application for banana wilt disease based on banana extracellular DNA, belonging to the field of plant disease biological control technology. The method includes: extracting total DNA from healthy banana tissue, obtaining esDNA fragments of 100-500 bp size through physical shearing; preparing the esDNA into a working solution, and applying it to banana plants via root irrigation. This invention utilizes banana-derived esDNA as an immune elicitor, which not only directly inhibits the mycelial growth of *Fusarium oxysporum* var. *cube* (especially Foc TR4), but also systematically activates the immune response of banana plants, enhances the activity of their defensive enzymes, and reduces the accumulation of reactive oxygen species, thereby significantly reducing rhizosphere colonization of the pathogen, alleviating yellowing and wilting symptoms, and improving plant survival rate and biomass. This method is environmentally friendly, highly safe, and easy to operate, providing a new green solution for the sustainable management of banana wilt disease.

This invention relates to the field of inducible hormone dosage regulation, and more particularly to a method and system for regulating plant growth inducible hormone dosage based on sample analysis. The method includes the following steps: collecting rice samples from multiple cultivation environments, monitoring photosynthesis to obtain response sequences over multiple periods; eliminating inefficient samples based on the response sequences to obtain response curves for candidate samples; identifying growth stages from the response curves of the candidate samples to obtain growth stage curves; performing inducible hormone gain analysis based on the growth stage curves to obtain inducible hormone sensitivity values ​​for different samples; and iteratively calculating and risk-screening the inducible hormone combination ratio based on the inducible hormone sensitivity values ​​of different samples to output the optimal dosage ratio. This invention improves the photosynthetic efficiency and enhances growth stability of rice.

The present disclosure relates to elicitors that defend responses in useful plants to establish a natural defense system of a plant against plant pests, where the elicitors are nonanoic acid or derivatives thereof.

The invention provides elicitor peptide LilyPep1 and application of the elicitor peptide LilyPep1 in promotion of lily tissue culture and rapid propagation, and relates to the technical field of plant tissue culture, the amino acid sequence of the LilyPep1 is as shown in SEQ ID NO.2, when the elicitor peptide LilyPep1 is applied to lily tissue culture, the tissue culture efficiency is remarkably improved, and the budding rate, the multiplication coefficient and the rooting rate in primary culture, subculture, multiplication culture and rooting culture are remarkably increased; compared with the lilium brownii without the polypeptide, the lilium brownii without the polypeptide is remarkably improved by 17.59%, 80.56% and 25.33% respectively, the elicitor peptide LilyPep1 is applied to lilium brownii tissue culture for the first time, and a brand-new technical approach for promoting lilium brownii tissue culture and rapid propagation is created.

The invention discloses a preparation method of hydroxyphenyl peptide and application of the hydroxyphenyl peptide in agriculture, relates to the technical field of plant growth regulators, and innovatively combines the hydroxyphenyl peptide with a conventional plant growth regulator to realize synergistic interaction of multiple physiological functions. The hydroxyphenyl peptide is prepared by a chemical method or a biological method; the hydroxyphenyl peptide and the compound sodium nitrophenolate are compounded for use under a specific concentration, so that the growth and development of crops can be remarkably promoted, and the yield of the crops is increased; the hydroxyphenyl peptide, the 5-aminolevulinic acid and the S-abscisic acid are compounded for use under a specific concentration, so that advanced coloring of solanaceous and berry crops such as tomatoes, strawberries and peppers can be promoted, fruit expansion is promoted, and fruit quality is improved; the growth-promoting and color-changing-promoting composition provided by the invention can obviously promote the growth and development of plants at a proper concentration, improve the yield and quality of crops, realize the dual effects of growth promotion and color changing promotion at a low dosage, is environment-friendly, and has important agricultural production application value.

Hypersensitive response eliciting peptides exhibiting improved solubility, stability, resistance to chemical degradation, or a combination of these properties are disclosed. Also disclosed are uses of these peptides or fusion polypeptides or DNA constructs encoding the same for modulating plant biochemical signaling, conferring disease resistance to a plant, enhancing plant growth, conferring tolerance to a plant to a biotic stress, conferring tolerance and resistance to a plant to an abiotic stress, conferring desiccation resistance to a cutting taken from an ornamental plant, conferring postharvest disease resistance or postharvest desiccation resistance to a fruit or vegetable, or enhancing the postharvest life of a fruit or vegetable.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an effect of sweet orange elicitor peptide CsPep in promoting regeneration of citrus explants and / or improving canker resistance. The invention provides a sweet orange elicitor peptide CsPep. The amino acid sequence of the sweet orange elicitor peptide CsPep is as shown in SEQ ID NO: 3. The peptide molecule can simultaneously play dual functions of promoting growth and inducing immunity, and the peptide is conservative in function and broad-spectrum in action in different citrus varieties, and has specificity to the optimal application concentration of different varieties, and the technical scheme disclosed by the invention is beneficial to accurate application of the sweet orange elicitor peptide.

The invention belongs to the technical field of bioengineering, and particularly relates to a protein elicitor SyCD1 and application thereof. According to the invention, a protein elicitor SyCD1 gene is obtained by cloning from Rabdolothrix poplar Hhs.015, the nucleotide sequence of the protein elicitor SyCD1 gene is as shown in SEQ ID No: 1, and the amino acid sequence of the protein coded by the protein elicitor SyCD1 gene is as shown in SEQ ID No: 2. The efficacy of the unknown functional protein-protein elicitor SyCD1 is discussed and studied for the first time, the unknown functional protein-protein elicitor SyCD1 is applied to improvement of plant disease resistance, and tests prove that the SyCD1 protein induces plant active oxygen accumulation and callose bursting, regulates plant defense gene expression and improves plant disease resistance. The invention provides a new biological material for improving the disease resistance of plants.

This disclosure provides a method for alleviating the damaging effects of D. maidis in corn crops, which involves the use of plant defense elicitors such as jasmonates and salicylates.

The invention discloses plant disease resistance inducing activity of a thyme extract and application of the thyme extract in preparation of a plant immune resistance inducer. The resistance inducer disclosed by the invention can activate systematic resistance of tobacco, and has a good prevention and control effect on various diseases. The thyme extract accounts for 10-50% of the plant resistance inducer in percentage by mass, and can be prepared into soluble liquid, soluble powder, water dispersible granules, emulsion in water, microemulsion and other preparation types. The bactericidal composition is used for preventing and controlling or relieving various plant fungi, bacteria and virus diseases such as tobacco mosaic virus diseases, strawberry gray mold, cucumber powdery mildew and the like on crops such as tobacco, strawberries and cucumbers. According to the present invention, the change of the thyme extract in vivo physiological indexes and disease resistance related genes caused by the thyme extract is determined by using biological activity determination, physiological and biochemical test and other methods, the plant disease resistance inducing activity of the thyme extract is determined, and the plant immune resistance inducer using the thyme extract as the active component is prepared and is used in the field.

The invention provides a preparation method of peony and ginseng peptide for enhancing immunity and tonifying qi and blood, and belongs to the field of nutritious food. The composition comprises a peony peptide extract and ginseng polypeptide, the peony peptide extract is prepared by the following steps: soaking peony seeds in an elicitor solution, and fermenting with rhizopus oligosporus; and separating and purifying to obtain the peony peptide extract. Fresh peony seeds are soaked in the elicitor, biological stress is simulated to activate the defensive reaction of the peony seeds, and protein accumulation is induced; the rhizopus oligosporus is fermented for biotransformation, protein degradation is promoted, and active substances are possibly generated. And carrying out subsequent extraction to obtain the peony peptide extract. The extract and ginseng polypeptide are mixed in proportion, bioactive components from different sources are integrated, small molecule peptide is easy to absorb, and the immune regulation function is enhanced through the multi-target synergistic effect. The whole process is based on plant physiological response and biotransformation, and the preparation efficiency and quality of the peptide are optimized, so that the final composition has the effects of enhancing immunity and tonifying qi and blood.

The invention relates to the field of prevention and treatment of plant germs, and discloses a plant immune resistance inducer as well as a preparation method and application thereof. The invention provides a novel plant immune resistance inducer which is shown as a formula (1), a preparation method of the novel plant immune resistance inducer comprises the following steps: mixing validated hydroxylamine A, R '-COOH and lipase for catalytic reaction, and R' is selected from at least one of phenyl, substituted phenyl, pyridyl, substituted pyridyl and C1-C21 aliphatic hydrocarbon groups. The plant immune resistance inducer is diluted with water according to a certain proportion and then sprayed on tomato leaf surfaces, the tomato can be induced to generate disease resistance, plant diseases can be effectively reduced, the action mechanism is to induce generation of defensive reaction in plants instead of directly acting on pathogenic bacteria, and the plant immune resistance inducer has the advantages of being safe and reliable. Therefore, variation and drug resistance of pathogenic bacteria are avoided. The problems that in the prior art, chemical pesticides are adopted for preventing and treating tomato gray mold, drug resistance is prone to being generated, and safety is not achieved are solved.