41 results about "Elicitor" patented technology

The application provides a polypeptide, a plant immune elicitor and application thereof. The polypeptide has 10 or 11 amino acid residues; the amino acid at the first position of the polypeptide is serine, and the amino acids at the 9th and 10th positions are both cysteine; if the polypeptide has 11 amino acid residues, the amino acid at the 11th position of the polypeptide is asparagine. The polypeptide can solve the problem that there is no small peptide secreted by gramineae plants in the prior art, and is suitable for the field of plant immune elicitors.

The application discloses a plant immunity elicitor protein PpAE4 and application thereof, the protein can induce plant resistance, and can be applied to the field of plant disease prevention and treatment as a plant immunity activator. The amino acid sequence of the protein is shown as SEQ ID NO:2. High-concentration protein can be obtained by using an engineering strain for expression, and the protein can be used for treating plants to improve the disease resistance of the plants to soybean rust fungi, and the required concentration is low, the effect is quick, and the action time is long. The PpAE4 provides a new way for improving the disease resistance of plants and preventing and treating plant diseases.

The invention relates to the technical field of agricultural biology, in particular to cotton bollworm salivary protein Ha22, a recombinant vector, recombinant bacteria and application of the cotton bollworm salivary protein Ha22. The invention provides a cotton bollworm salivary protein Ha22. The amino acid sequence of the cotton bollworm salivary protein Ha22 is as shown in SED ID NO. 1. Transient expression of the Ha22 protein on tobacco can cause tobacco cell necrosis, stable overexpression of the Ha22 protein on cotton plants can induce cotton defensive gene response and positively regulate plant insect resistance, and the Ha22 protein has great application value for cotton insect-resistant breeding. In addition, the protein has the active characteristic of an elicitor, so that the purpose of green prevention and control can be achieved by spraying whole protein to plants through in-vitro expression and inducing plant resistance.

The invention discloses application of pseudopenicillin variotii in preparation of a resistance inducer for preventing and treating plant diseases, and belongs to the technical field of plant protection. The invention provides an application of Panax variotii in prevention and treatment of plant diseases, the application concentration of the Panax variotii is 0.1 microgram / mL-1 microgram / mL, a plant resistance inducer containing the Panax variotii can be sprayed on plant leaves or fruit surfaces and has a remarkable prevention effect on anthrax leaf blight, and the higher the concentration is, the better the prevention effect is; the pseudopenicillin variotii can promote synthesis or activity improvement of catalase, peroxidase, polyphenol oxidase, glutathione S-transferase and superoxide dismutase in apple bodies, and also can remarkably induce up-regulation expression of disease-resistant related genes such as MdPR2 and MdPR5, so that the disease resistance, the antioxidant level and the plant immunity of apples are enhanced; a novel and efficient plant resistance inducer application scheme is provided for apple disease control and physiological regulation.

The application discloses application of an immune elicitor protein FvXynC and a protein combination in plant disease resistance, and belongs to the technical field of plant immune elicitors. A new immune elicitor protein FvXynC is found from F. pseudograminearum, and the immune elicitor protein has the core functions of activating plant immune response and improving plant resistance to F. pseudograminearum, can be used as an efficient plant immune inducer, provides a new technical path for plant disease resistance improvement and green plant disease control, and has important agricultural application value. The FvXynC and the immune elicitor protein FvGH43A are compounded, so that the resistance level of corn to F. pseudograminearum can be synergistically improved, and the problem that the plant disease control effect of a single immune elicitor protein is limited is solved.

This invention relates to the field of plant disease biological control technology, and particularly to a Fusarium oxysporum cell wall extract, its preparation method, and its application. The extract is derived from the polysaccharide fraction of the cell wall of Fusarium oxysporum spores or hyphae, and contains at least one pathogen-associated molecular pattern (PAMP) component. This component is a non-protein, thermally stable, species-nonspecific elicitor capable of forming an aqueous dispersion system. The extract of this invention can be recognized by plant PRRs and activate PTI immunity. Rehmannia glutinosa tubers treated with the extract showed a significantly increased germination rate after inoculation with Fusarium oxysporum. It also induces superoxide anion production, upregulates the activities of superoxide dismutase, peroxidase, and catalase, and enriches differentially expressed genes in the MAPK signaling pathway, jasmonic acid metabolism pathway, and phenylpropane biosynthesis pathway. Furthermore, the extract can directly inhibit Fusarium oxysporum colony expansion, conidia production, and mycelial growth. Extracts derived from spores or hyphae show no significant difference in inducing disease resistance, and the raw materials are flexible and easy to industrialize. This extract is derived from the cell wall of the pathogen itself, making it environmentally friendly and free from the risk of chemical pesticide residues. It is suitable for the green control of root rot disease in Rehmannia glutinosa.

The invention belongs to the technical field of biological prevention and control, and provides application of N-palmitoyl aspartic acid in improvement of resistance of rice bacterial blight, which is characterized in that N-palmitoyl aspartic acid is prepared into a resistance inducer for prevention and control of bacterial blight pathogen infection in a three-leaf one-core period of rice. The inhibition effect of the N-palmitoyl aspartic acid on the Xanthomonas oryzae pv. Oryzae is evaluated, and the result shows that the N-palmitoyl aspartic acid effectively reduces the gene expression of the Xanthomonas oryzae pv. Oryzae, inhibits the growth and propagation of the Xanthomonas oryzae pv. Oryzae, improves the resistance of rice, can replace or reduce the use of chemical bactericides, and is environment-friendly.

The present invention relates to a method for producing steviol glycosides of Stevia rebaudiana, which method comprises the following steps: preparing the Stevia rebaudiana nodal explants having two internodes; propagating the explants in temporary immersion systems (SETIS) with controlled immersion cycles; adding an elicitor after 21 days of culture; and transferring to a liquid culture medium enriched with growth regulators and nutrients that are essential to plant growth and development.

The invention discloses a plant immune induced resistance protein Ps131903 and application thereof. The amino acid sequence of the immune induced resistance protein Ps131903 is shown as SEQ ID NO.2. The protein Ps131903 can reduce the harm of phytophthora sojae and pythium ultimum to soybeans, reduce the harm of phytophthora capsici to chilies and tomatoes and reduce the harm of pyricularia grisea to rice. The compound has the advantages of low use concentration, quick effect and broad spectrum, and provides a new way for improving the plant resistance, thereby having wide application prospects in agricultural production.

This invention discloses a green control method and application for banana wilt disease based on banana extracellular DNA, belonging to the field of plant disease biological control technology. The method includes: extracting total DNA from healthy banana tissue, obtaining esDNA fragments of 100-500 bp size through physical shearing; preparing the esDNA into a working solution, and applying it to banana plants via root irrigation. This invention utilizes banana-derived esDNA as an immune elicitor, which not only directly inhibits the mycelial growth of *Fusarium oxysporum* var. *cube* (especially Foc TR4), but also systematically activates the immune response of banana plants, enhances the activity of their defensive enzymes, and reduces the accumulation of reactive oxygen species, thereby significantly reducing rhizosphere colonization of the pathogen, alleviating yellowing and wilting symptoms, and improving plant survival rate and biomass. This method is environmentally friendly, highly safe, and easy to operate, providing a new green solution for the sustainable management of banana wilt disease.

This invention relates to the field of inducible hormone dosage regulation, and more particularly to a method and system for regulating plant growth inducible hormone dosage based on sample analysis. The method includes the following steps: collecting rice samples from multiple cultivation environments, monitoring photosynthesis to obtain response sequences over multiple periods; eliminating inefficient samples based on the response sequences to obtain response curves for candidate samples; identifying growth stages from the response curves of the candidate samples to obtain growth stage curves; performing inducible hormone gain analysis based on the growth stage curves to obtain inducible hormone sensitivity values ​​for different samples; and iteratively calculating and risk-screening the inducible hormone combination ratio based on the inducible hormone sensitivity values ​​of different samples to output the optimal dosage ratio. This invention improves the photosynthetic efficiency and enhances growth stability of rice.

The present disclosure relates to elicitors that defend responses in useful plants to establish a natural defense system of a plant against plant pests, where the elicitors are nonanoic acid or derivatives thereof.

The invention discloses a preparation method of hydroxyphenyl peptide and application of the hydroxyphenyl peptide in agriculture, relates to the technical field of plant growth regulators, and innovatively combines the hydroxyphenyl peptide with a conventional plant growth regulator to realize synergistic interaction of multiple physiological functions. The hydroxyphenyl peptide is prepared by a chemical method or a biological method; the hydroxyphenyl peptide and the compound sodium nitrophenolate are compounded for use under a specific concentration, so that the growth and development of crops can be remarkably promoted, and the yield of the crops is increased; the hydroxyphenyl peptide, the 5-aminolevulinic acid and the S-abscisic acid are compounded for use under a specific concentration, so that advanced coloring of solanaceous and berry crops such as tomatoes, strawberries and peppers can be promoted, fruit expansion is promoted, and fruit quality is improved; the growth-promoting and color-changing-promoting composition provided by the invention can obviously promote the growth and development of plants at a proper concentration, improve the yield and quality of crops, realize the dual effects of growth promotion and color changing promotion at a low dosage, is environment-friendly, and has important agricultural production application value.

Hypersensitive response eliciting peptides exhibiting improved solubility, stability, resistance to chemical degradation, or a combination of these properties are disclosed. Also disclosed are uses of these peptides or fusion polypeptides or DNA constructs encoding the same for modulating plant biochemical signaling, conferring disease resistance to a plant, enhancing plant growth, conferring tolerance to a plant to a biotic stress, conferring tolerance and resistance to a plant to an abiotic stress, conferring desiccation resistance to a cutting taken from an ornamental plant, conferring postharvest disease resistance or postharvest desiccation resistance to a fruit or vegetable, or enhancing the postharvest life of a fruit or vegetable.

The invention belongs to the technical field of plant genetic engineering, and particularly relates to an effect of sweet orange elicitor peptide CsPep in promoting regeneration of citrus explants and / or improving canker resistance. The invention provides a sweet orange elicitor peptide CsPep. The amino acid sequence of the sweet orange elicitor peptide CsPep is as shown in SEQ ID NO: 3. The peptide molecule can simultaneously play dual functions of promoting growth and inducing immunity, and the peptide is conservative in function and broad-spectrum in action in different citrus varieties, and has specificity to the optimal application concentration of different varieties, and the technical scheme disclosed by the invention is beneficial to accurate application of the sweet orange elicitor peptide.

The invention discloses plant disease resistance inducing activity of a thyme extract and application of the thyme extract in preparation of a plant immune resistance inducer. The resistance inducer disclosed by the invention can activate systematic resistance of tobacco, and has a good prevention and control effect on various diseases. The thyme extract accounts for 10-50% of the plant resistance inducer in percentage by mass, and can be prepared into soluble liquid, soluble powder, water dispersible granules, emulsion in water, microemulsion and other preparation types. The bactericidal composition is used for preventing and controlling or relieving various plant fungi, bacteria and virus diseases such as tobacco mosaic virus diseases, strawberry gray mold, cucumber powdery mildew and the like on crops such as tobacco, strawberries and cucumbers. According to the present invention, the change of the thyme extract in vivo physiological indexes and disease resistance related genes caused by the thyme extract is determined by using biological activity determination, physiological and biochemical test and other methods, the plant disease resistance inducing activity of the thyme extract is determined, and the plant immune resistance inducer using the thyme extract as the active component is prepared and is used in the field.

The invention provides a preparation method of peony and ginseng peptide for enhancing immunity and tonifying qi and blood, and belongs to the field of nutritious food. The composition comprises a peony peptide extract and ginseng polypeptide, the peony peptide extract is prepared by the following steps: soaking peony seeds in an elicitor solution, and fermenting with rhizopus oligosporus; and separating and purifying to obtain the peony peptide extract. Fresh peony seeds are soaked in the elicitor, biological stress is simulated to activate the defensive reaction of the peony seeds, and protein accumulation is induced; the rhizopus oligosporus is fermented for biotransformation, protein degradation is promoted, and active substances are possibly generated. And carrying out subsequent extraction to obtain the peony peptide extract. The extract and ginseng polypeptide are mixed in proportion, bioactive components from different sources are integrated, small molecule peptide is easy to absorb, and the immune regulation function is enhanced through the multi-target synergistic effect. The whole process is based on plant physiological response and biotransformation, and the preparation efficiency and quality of the peptide are optimized, so that the final composition has the effects of enhancing immunity and tonifying qi and blood.

The invention relates to the field of prevention and treatment of plant germs, and discloses a plant immune resistance inducer as well as a preparation method and application thereof. The invention provides a novel plant immune resistance inducer which is shown as a formula (1), a preparation method of the novel plant immune resistance inducer comprises the following steps: mixing validated hydroxylamine A, R '-COOH and lipase for catalytic reaction, and R' is selected from at least one of phenyl, substituted phenyl, pyridyl, substituted pyridyl and C1-C21 aliphatic hydrocarbon groups. The plant immune resistance inducer is diluted with water according to a certain proportion and then sprayed on tomato leaf surfaces, the tomato can be induced to generate disease resistance, plant diseases can be effectively reduced, the action mechanism is to induce generation of defensive reaction in plants instead of directly acting on pathogenic bacteria, and the plant immune resistance inducer has the advantages of being safe and reliable. Therefore, variation and drug resistance of pathogenic bacteria are avoided. The problems that in the prior art, chemical pesticides are adopted for preventing and treating tomato gray mold, drug resistance is prone to being generated, and safety is not achieved are solved.

The invention discloses application of methyl carboline acid in preparation of a resistance inducer for preventing and treating plant diseases, and belongs to the technical field of plant protection. The methyl carboline acid is used for preventing and treating plant diseases, the methyl carboline acid can efficiently prevent and treat the leaf blight of anthrax and can inhibit the leaf blight of anthrax, and the resistance inducer containing the methyl carboline acid can be directly sprayed to the surfaces of plant leaves or fruits and is easy and convenient to use and easy to popularize. The methyl carboline acid can promote synthesis or activity improvement of catalase, peroxidase, polyphenol oxidase, glutathione S-transferase and superoxide dismutase in plants, and also can remarkably induce up-regulation expression of MdPR2 and MdPR5 disease-resistant genes, so that the disease resistance, the antioxidant level and the plant immunocompetence of the plants are enhanced; the invention provides a novel and efficient application scheme of the plant resistance inducer for plant disease control and physiological regulation, and the plant resistance inducer has a wide market application prospect.

The application discloses application of a strigolactone inhibitor in prevention and treatment of TMV viruses and relates to the technical field of molecular biology. The application inoculates TMV after treating Nicotiana attenuata with a strigolactone (SL) hormone, performs transcriptome sequencing, analyzes gene conditions of difference changes, and performs qPCR verification on differentially expressed genes; research on SL-induced promotion of Nicotiana attenuata to TMV infection is carried out, and it is clear that the SL has the ability to inhibit tobacco resistance to tobacco mosaic virus (TMV); it is determined that the SL is a plant hormone for inhibiting plant defense reaction, and the SL inhibitor Tis108 has the ability to enhance tobacco resistance to TMV, and it is determined that the Tis108 is a plant hormone inhibitor for promoting plant defense reaction. The application lays a foundation for further research and utilization of the SL hormone, development of a new type of plant elicitor, safe and efficient prevention and treatment of plant diseases.

The invention discloses a phytophthora pini effector protein PpinAvh23 as well as a coding gene and application thereof, and belongs to the technical field of molecular biology. The amino acid sequence of the protein is as shown in SEQ ID NO. 1. Experiments show that the protein can specifically inhibit programmed death of plant cells induced by pathogenic bacteria elicitors, and it is proved that the protein can promote pathogen infection as a toxic effector in plants. The invention also provides a gene (SEQ ID NO.2) for coding the protein. The protein and the coding gene thereof can be used as a key molecular tool and a screening target for researching a plant-pathogen interaction mechanism, and a basis is provided for developing a new strategy for preventing and controlling plant diseases.

The invention discloses a tea tree exogenous induced insect-resistant agent based on synergistic interaction of jasmonic acid methyl ester and salicylic acid as well as a preparation method and application of the tea tree exogenous induced insect-resistant agent, and belongs to the technical field of biological control. Based on the synergistic interaction of jasmonic acid methyl ester and salicylic acid, tea geometrid and tea lesser leafhopper are repelled, and parasitic wasps and hoverflies in a tea garden are lured. Synergistic interaction is achieved through the specific proportion of jasmonic acid methyl ester and salicylic acid, a tea tree jasmonic acid and salicylic acid double insect-resistant signal pathway is activated, the total amount and the component fraction of volatile matter generated through induction are remarkably higher than those generated through single elicitor treatment, the population number of pest natural enemy parasitic wasps in a tea garden can be increased by 1.52 times through field tests, and the pest control effect is good. The number of hoverfly populations is increased by 1.33 times, the number of tea lesser leafhopper populations is reduced by 40.5%, the number of tea geometrid populations is reduced by 60%, and the insect-resistant effect can be maintained for 15 days.

The invention provides application of soybean rust fungus elicitor PpE1 protein in inducing plant immune response and / or improving plant resistance, and belongs to the technical field of elicitor protein application. The amino acid sequence of the soybean rust fungus elicitor PpE1 protein is as shown in SEQ ID NO. 2. The invention discovers the application of the soybean rust fungus protein elicitor PpE1 in inducing plant immune response and improving plant resistance for the first time. The PpE1 can cause the hypersensitive reaction of the nicotiana benthamiana and induce active oxygen in the nicotiana benthamiana to burst out; the protein can induce immune response of soybeans, including burst of active oxygen, activation of MAPK and expression of immune genes, and promote resistance of the soybeans to rust fungi; the heterologous expression of the PpE1 can also improve the resistance of the soybean to rust fungi. The invention provides a new way for improving the disease resistance of plants, and has a wide application prospect in agricultural production.

The invention discloses citral-amino-oligosaccharide Schiff base and application of the citral-amino-oligosaccharide Schiff base as a plant resistance inducer. The citral-amino-oligosaccharide Schiff base disclosed by the invention can activate systematic resistance of tobaccos, and has a good prevention and control effect on various diseases. According to the present invention, the plant disease resistance inducing activity, the plant stress resistance inducing activity and the plant growth promoting activity of the citral-amino-oligosaccharide Schiff base are determined by using biological activity determination, physiological and biochemical testing and other methods, and the pesticide preparation, the auxiliary agent and the biostimulant using the citral-amino-oligosaccharide Schiff base as the active component are prepared; the method is applied in fields.

The invention discloses a stability-enhanced plant immune resistance inducer based on mesoporous silica coupled immune protein and application of the stability-enhanced plant immune resistance inducer, and belongs to the technical field of plant disease control. According to the invention, fluorescence visualization and nano confinement stability enhancement technology are creatively integrated in a mesoporous silica protein delivery system, and two core problems of'difficult tracking 'and'poor stability' in research and application of plant immune induced resistance protein are successfully solved. The system not only can be used as an efficient plant vaccine delivery product, but also becomes a powerful basic research platform due to the visual characteristic, provides key technical support for optimizing the delivery efficiency and illuminating the induced resistance mechanism, and has great application value and market prospect in the field of green agriculture.

The invention discloses a plant salt-tolerant resistance inducer as well as a screening method and application thereof. The resistance inducer comprises sodium 2-(4-methoxyphenoxy) propionate, 4-methylene-L-glutamine, 2-octenoic acid, 2E, 7-octadiene aldehyde, threonyl isoleucine, propyl 2, 4-decadienoate, guanosine and indene-1-alcohol, and the resistance inducer is prepared from the raw materials according to a specific ratio. The screening method comprises the following steps: collecting root exudates of hermaphrodite salt-tolerant plants, and carrying out non-targeted metabonomics analysis; screening metabolites in positive correlation with salt stress in combination with growth phenotypes and physiological indexes; the resistance inducer is obtained by compounding according to a determined proportion. The resistance inducer can remarkably improve the salt tolerance of plants such as willows, corns, wheat and oilseed rapes, promote root growth, improve ion balance and increase biomass, and has the advantages of being environmentally friendly, low in cost and wide in application.

The invention discloses application of N-stearoyl glycine in improving rice blast resistance, and relates to the technical field of plant immune resistance inducers and green agricultural invests.It is found for the first time that after plants are treated with low-concentration N-stearoyl glycine, systematic resistance of rice to rice blast can be induced by activating an OsNPR1-OsPR2-OsMAPK6 module, and therefore the application of N-stearoyl glycine in improving rice blast resistance is achieved. The N-stearoyl glycine can be used as an effective component with plant immunity resistance. The plant immune resistance inducer prepared by taking N-stearoylglycine as an effective component can be used for preventing and treating by a spraying method, is high in operability, simple and quick, and can be used for effectively preventing and treating expansion and spreading of diseases. In addition, the N-stearoyl glycine is an animal endogenous metabolite, is easy to degrade and has no residual risk; and the N-stearoyl glycine is low in cost and conforms to the current agricultural sustainable development direction.

The application belongs to the technical field of plant disease prevention and treatment, and discloses a flagellin and application of the flagellin in a plant immune elicitor. The flagellin is separated and extracted from Bacillus amyloliquefaciens Ba168, has good bacteriostatic activity, and has an immune induction effect on tobacco. The application realizes mass expression and purification of the flagellin by using genetic engineering technology. The flagellin can induce a plant immune response, can induce plant cell necrosis, plant active oxygen burst, and an increase of plant defense enzyme activity. The flagellin can induce plant resistance to PVX, and provides a new type of elicitor material for prevention and treatment of PVX.

The present application relates to compositions comprising one or more elicitor proteins, polypeptides, or peptides that induce defense responses in plants, thereby enhancing resistance to biotic and abiotic stress and promoting growth. These compositions can be applied to seeds, plants, or soil to improve crop performance and yield. The invention further includes transgenic plants expressing nucleic acids encoding elicitor proteins. It further discloses methods for controlling plague infestation improving the yield of a plant by applying the composition comprising the elicitor protein.