109 results about "Elicitor" patented technology

The invention discloses a plant immune induced resistance protein PpAE4 and application thereof, and the protein can induce plant resistance and can be used as a plant immune activating factor to be applied to the field of prevention and treatment of plant diseases. The amino acid sequence of the protein is as shown in SEQ ID NO: 2. High-concentration protein can be obtained through expression of the engineering strain, the disease resistance of plants to soybean rust germs can be improved when the protein is used for treating the plants, and the protein is low in needed concentration, quick in effect taking and long in acting time. The PpAE4 provides a new way for improving plant disease resistance and preventing and treating plant diseases.

The invention provides application of small peptides in preventing and treating plant diseases and promoting plant growth. The structural formula of the small peptide is shown in the specification. Experimental results show that the small peptide shown in the formula A has the effects of improving the disease resistance of plants and regulating the growth of the plants. The small peptide not only can be used as a plant immune resistance inducer at low concentration to improve the immunocompetence of plants for resisting pathogen invasion, but also can promote plant growth, and has a good application prospect. R1-R2-beta-Ala-Trp-Gly-NH2 Formula A

The present application relates to the technical field of elicitor peptide MsPep and its application, and discloses the use of elicitor peptide MsPep in improving the genetic transformation efficiency of alfalfa, wherein the application takes the leaf of alfalfa as an explant, introduces an exogenous gene through Agrobacterium tumefaciens, and adds the elicitor peptide MsPep in each stage of genetic transformation, so as to significantly improve the dedifferentiation rate, the out-growth rate, the differentiation rate, the out-bud rate, the rooting rate and the transformation efficiency, and shorten the transformation period, and the present application has application prospect in improving the genetic transformation of alfalfa and other plants.

The present invention relates to a composition comprising components (A) and (B) as active ingredients, wherein component (A) comprises a Streptomyces and component (B) is a compound selected from the group consisting of sulphur, phosphonate, copper hydroxide, copper oxychloride, bicarbonate, Bacillus sp, Trichoderma sp, aureobasidin A, jawsamycin, kasugamycin, natamycin, a polysaccharide elicitor, a plant biostimulant (PBS) comprising an algal extract, a plant extract and / or vinasse, orange oil, and / or tea tree oil. The invention further relates to a method of controlling or preventing phytopathogenic diseases, preferably phytopathogenic fungi, on a plant or on plant propagation material and / or on harvested food crops, which comprises applying to the plant, on plant propagation material, the locus thereof, and / or on harvested food crops the composition according to the present invention.

The invention discloses application of an arabidopsis proPep1 mutant gene and an encoded protein thereof in resisting potyvirus viruses, and belongs to the technical field of plant genetic engineering. In order to solve the technical problems that in the prior art, potyvirus resistance resources are few, prevention and control are difficult, and environmental pollution is easily caused by chemical prevention and control of insect propagation mediators, the invention provides an arabidopsis proPep1 mutant gene and an application of an encoding protein thereof in resisting potyvirus viruses, the nucleotide sequence of the arabidopsis proPep1 mutant gene is as shown in SEQ ID NO.2, and the encoding protein of the arabidopsis proPep1 mutant gene has a nucleotide sequence as shown in SEQ ID NO.1. The arabidopsis proPep1 mutant gene has a nucleotide sequence as shown in SEQ ID NO.2. And a coding protein sequence is shown as SEQ ID NO.4, and a plant elicitor small peptide Pep1 expression vector mature by NIa-Pro protease hydrolysis and a transgenic disease-resistant plant are constructed. The disease-resistant system provided by the invention achieves the purpose of enhancing the potyvirus virus resistance of plants, and can be applied to prevention and treatment of diseases caused by potyvirus viruses.

The disclosure relates to methods and compositions for altering the production of one or more secondary plant metabolites comprising applying an effective amount of at least one elicitor, wherein the elicitor is a jasmonate or a salicylate, and combinations thereof. The disclosure further teaches compositions comprising effective amounts of the elicitors disclosed here. The disclosure further relates to methods and compositions for controlling plant pathogens, such as fungal pathogens.

The invention belongs to the field of microbiology and molecular biology, and particularly relates to a method for extracting myxococcus flavus dormant mucospore RNA (Ribonucleic Acid). The method specifically comprises the following steps: taking a certain amount of myxococcus flavus liquid, and inducing sporocarp development by using a common starvation induction method; scraping the formed sporocarp structure, resuspending with sterile water, and destroying the sporocarp structure and mucospore coating by using a certain proportion of organic reagent; centrifuging, taking a water phase, adding an acetate buffer solution with a certain concentration, and precipitating with isopropanol to obtain nucleic acid substances; and removing DNA by using a conventional kit to obtain RNA. Aiming at the characteristic that the mucospore RNA is difficult to extract, the invention develops a simple, convenient and efficient method for extracting the mucospore RNA, and provides an effective way for deeply researching the ecological characteristics of the myxobacteria.

The application provides a polypeptide, a plant immune elicitor and application thereof. The polypeptide has 10 or 11 amino acid residues; the amino acid at the first position of the polypeptide is serine, and the amino acids at the 9th and 10th positions are both cysteine; if the polypeptide has 11 amino acid residues, the amino acid at the 11th position of the polypeptide is asparagine. The polypeptide can solve the problem that there is no small peptide secreted by gramineae plants in the prior art, and is suitable for the field of plant immune elicitors.

The present disclosure relates to the elicitors of defensive responses in useful plants to build up the plants' natural defense system against plant pathogens, wherein the elicitor is pelargonic acid, or a derivative thereof.

The invention discloses a broad-spectrum plant-source disease-resistant excitant for rice blast and a preparation method thereof, and belongs to the technical field of rice blast prevention and control, in the broad-spectrum plant-source disease-resistant excitant, potassium glutamate serves as amino acid salt, not only provides a nitrogen source for plants, but also participates in the metabolic process in the plants and enhances the stress resistance of the plants. S-allyl-L-cysteine is a sulfur-containing amino acid derivative, has antioxidant and disease-resistant activity, and can stimulate a defense mechanism of plants and resist infection of magnaporthe oryzae. 2-piperidinecarboxylic acid has the effect of inhibiting growth of pathogenic bacteria and can directly interfere with cell wall synthesis of magnaporthe oryzae, so that diseases are relieved. Chitosan as a biological polysaccharide can induce plants to generate resistant substances, enhance the strength of plant cell walls, form a physical barrier and prevent invasion of pathogenic bacteria. Salicylic acid is an important signal molecule in a plant body and can activate systematic resistance of the plant and improve the overall disease resistance.

The application discloses a plant immunity elicitor protein PpAE4 and application thereof, the protein can induce plant resistance, and can be applied to the field of plant disease prevention and treatment as a plant immunity activator. The amino acid sequence of the protein is shown as SEQ ID NO:2. High-concentration protein can be obtained by using an engineering strain for expression, and the protein can be used for treating plants to improve the disease resistance of the plants to soybean rust fungi, and the required concentration is low, the effect is quick, and the action time is long. The PpAE4 provides a new way for improving the disease resistance of plants and preventing and treating plant diseases.

The invention relates to the technical field of agricultural biology, in particular to cotton bollworm salivary protein Ha22, a recombinant vector, recombinant bacteria and application of the cotton bollworm salivary protein Ha22. The invention provides a cotton bollworm salivary protein Ha22. The amino acid sequence of the cotton bollworm salivary protein Ha22 is as shown in SED ID NO. 1. Transient expression of the Ha22 protein on tobacco can cause tobacco cell necrosis, stable overexpression of the Ha22 protein on cotton plants can induce cotton defensive gene response and positively regulate plant insect resistance, and the Ha22 protein has great application value for cotton insect-resistant breeding. In addition, the protein has the active characteristic of an elicitor, so that the purpose of green prevention and control can be achieved by spraying whole protein to plants through in-vitro expression and inducing plant resistance.

The invention discloses application of pseudopenicillin variotii in preparation of a resistance inducer for preventing and treating plant diseases, and belongs to the technical field of plant protection. The invention provides an application of Panax variotii in prevention and treatment of plant diseases, the application concentration of the Panax variotii is 0.1 microgram / mL-1 microgram / mL, a plant resistance inducer containing the Panax variotii can be sprayed on plant leaves or fruit surfaces and has a remarkable prevention effect on anthrax leaf blight, and the higher the concentration is, the better the prevention effect is; the pseudopenicillin variotii can promote synthesis or activity improvement of catalase, peroxidase, polyphenol oxidase, glutathione S-transferase and superoxide dismutase in apple bodies, and also can remarkably induce up-regulation expression of disease-resistant related genes such as MdPR2 and MdPR5, so that the disease resistance, the antioxidant level and the plant immunity of apples are enhanced; a novel and efficient plant resistance inducer application scheme is provided for apple disease control and physiological regulation.

The invention discloses a novel vegetable health magnesium resistance inducer as well as a preparation method and application thereof, the main components of the vegetable health magnesium resistance inducer are trimethylglycine (GB) and magnesium oxide nanoparticles (MgONPs), and the components have the characteristics of no toxicity, harmlessness, no pollution and the like. The invention finds that the'vegetable health magnesium 'resistance inducer can activate plant hormone signals, enhance plant immunity and resist virus diseases. The composition can be sprayed to vegetable crops before the high-incidence period of vegetable virus diseases to prevent and treat virus diseases, is simple and convenient, has strong operability, and can effectively improve the broad-spectrum resistance of plants to various vegetable virus diseases. Compared with traditional pesticides, the composition is more green and safer, meets the requirements of sustainable and healthy development of agriculture, and has huge application potential.

The invention relates to a method for culturing industrial cannabinoid and tanshinone high-yield industrial cannabinoid adventitious roots, and belongs to the technical field of adventitious root cultivation. The method comprises the following steps: performing induction treatment on the industrial cannabis sativa adventitious roots by adopting a yeast extract to obtain the industrial cannabis sativa adventitious roots subjected to induction treatment; the yeast extract is low in cost and abundant in raw material supply, and can stimulate industrial cannabis sativa adventitious roots to synthesize more target metabolites as an elicitor; the industrial cannabis sativa adventitious roots subjected to induction treatment are subjected to scale-up culture by utilizing a bioreactor, so that cell proliferation and growth of the adventitious roots are promoted, and the biomass of the adventitious roots is increased; meanwhile, the adventitious roots are promoted to accumulate more target metabolites, and at the moment, the yields of cannabinoid phenol, cannabidiol, tetrahydrotanshinone I, tanshinone IIA and dihydrotanshinone I in the adventitious roots are up to 80.65 mg / L DW, 53.58 mg / L DW, 103.76 mg / L DW, 21.71 mg / L DW and 5.71 mg / L DW respectively. According to the method, the operation steps and the labor intensity are reduced, the defects of low biomass and low cannabinoid yield in the existing industrial cannabinoid adventitious root culture technology are overcome, and the industrial cannabinoid and tanshinone high-yield industrial cannabinoid adventitious roots can be economically and efficiently cultured all the year round.

The application discloses application of an immune elicitor protein FvXynC and a protein combination in plant disease resistance, and belongs to the technical field of plant immune elicitors. A new immune elicitor protein FvXynC is found from F. pseudograminearum, and the immune elicitor protein has the core functions of activating plant immune response and improving plant resistance to F. pseudograminearum, can be used as an efficient plant immune inducer, provides a new technical path for plant disease resistance improvement and green plant disease control, and has important agricultural application value. The FvXynC and the immune elicitor protein FvGH43A are compounded, so that the resistance level of corn to F. pseudograminearum can be synergistically improved, and the problem that the plant disease control effect of a single immune elicitor protein is limited is solved.

This invention relates to the field of plant disease biological control technology, and particularly to a Fusarium oxysporum cell wall extract, its preparation method, and its application. The extract is derived from the polysaccharide fraction of the cell wall of Fusarium oxysporum spores or hyphae, and contains at least one pathogen-associated molecular pattern (PAMP) component. This component is a non-protein, thermally stable, species-nonspecific elicitor capable of forming an aqueous dispersion system. The extract of this invention can be recognized by plant PRRs and activate PTI immunity. Rehmannia glutinosa tubers treated with the extract showed a significantly increased germination rate after inoculation with Fusarium oxysporum. It also induces superoxide anion production, upregulates the activities of superoxide dismutase, peroxidase, and catalase, and enriches differentially expressed genes in the MAPK signaling pathway, jasmonic acid metabolism pathway, and phenylpropane biosynthesis pathway. Furthermore, the extract can directly inhibit Fusarium oxysporum colony expansion, conidia production, and mycelial growth. Extracts derived from spores or hyphae show no significant difference in inducing disease resistance, and the raw materials are flexible and easy to industrialize. This extract is derived from the cell wall of the pathogen itself, making it environmentally friendly and free from the risk of chemical pesticide residues. It is suitable for the green control of root rot disease in Rehmannia glutinosa.

The present invention discloses a cyclic sugar phosphate compound and its application. The compound is shown in Formula I. Experimental studies have confirmed that the compound can provide carbon source nutrition for Xanthomonas bacteria and has the ability to broadly inhibit plant basal defense responses induced by various immune elicitors, including reactive oxygen species bursts and upregulated expression of pathogenesis-related (PR) genes, providing an effective tool for studying plant immune signaling pathways. #imgabs0#

The invention belongs to the technical field of biological prevention and control, and provides application of N-palmitoyl aspartic acid in improvement of resistance of rice bacterial blight, which is characterized in that N-palmitoyl aspartic acid is prepared into a resistance inducer for prevention and control of bacterial blight pathogen infection in a three-leaf one-core period of rice. The inhibition effect of the N-palmitoyl aspartic acid on the Xanthomonas oryzae pv. Oryzae is evaluated, and the result shows that the N-palmitoyl aspartic acid effectively reduces the gene expression of the Xanthomonas oryzae pv. Oryzae, inhibits the growth and propagation of the Xanthomonas oryzae pv. Oryzae, improves the resistance of rice, can replace or reduce the use of chemical bactericides, and is environment-friendly.

The invention relates to a preservative as well as a preparation method and application thereof. The preservative comprises a bacillus velezensis microbial inoculum and an elicitor cooperating with the microbial inoculum, and the addition amount of the elicitor is 0.01-0.1% of the total mass of the preservative. The preservative further comprises at least one of a protective agent, a preservative and an antioxidant; the bacillus velezensis microbial inoculum comprises the following components in parts by mass: 20-80 parts of a bacillus velezensis microbial inoculum, 0.001-0.1 part of an elicitor, 1-25 parts of a protective agent, 0.05-1 part of a preservative and 0.05-1 part of an antioxidant. The effective viable count in the bacillus velezensis microbial inoculum is greater than or equal to 10 < 9 > CFU / mL; the bacillus velezensis microbial inoculum comprises at least one of bacillus velezensis strains LTS-AF1, LTS-AF2, LTS-AF3 and LTS-AF4, and the bacillus velezensis microbial inoculum comprises at least one of bacillus velezensis strains LTS-AF1, LTS-AF2, the strain action effect is improved, the action time is prolonged, and the strain activity is stabilized.

The present invention discloses the application of the OsRbohI gene in regulating rice disease resistance, belonging to the field of biotechnology. The present invention verified multiple OsRbohI mutants with different genetic backgrounds and found that they almost did not produce reactive oxygen species bursts induced by plant immune elicitors, and their resistance to the most destructive rice blast fungus was significantly weakened; Arabidopsis transgenic strains were obtained using the floral dipping transformation method. The OsRbohI protein driven by the Arabidopsis AtRbohD promoter can cross-species complement the reactive oxygen species burst phenotype of the dicotyledonous model plant atrbohd mutant, indicating that OsRbohI can play a cross-species role. The present invention proves the important role of OsRbohI in rice disease resistance, provides new gene resources for rice disease resistance breeding, and lays a foundation for the selection of disease-resistant varieties for other species.

The invention relates to the technical field of rice induced disease resistance, and discloses a chemical elicitor for inducing rice sheath blight resistance and application, the chemical elicitor is prepared by the following steps: adding p-fluorophenoxyacetic acid into acetone, and uniformly stirring to obtain mother liquor; adding the compound, a tert-butylbenzene compound and terpinene into a mother solution, and uniformly stirring to obtain a composite mother solution; taking the composite mother liquor as a core material, and taking a ferric chloride aqueous solution and tannic acid as wall materials to obtain the chemical elicitor compound. The tert-butylbenzene compound and the terpinene assist the chemical elicitor on fluorophenoxyacetic acid, and through multiple inhibition on rhizoctonia solani, the resistance of rice to sheath blight disease can be induced, the disease area of the sheath blight disease is reduced, and infection of the rhizoctonia solani is inhibited; the water hyacinth seed extract contains a large number of phenolic hydroxyl structures and can be combined with the tert-butylbenzene compound and the p-fluorophenoxyacetic acid through hydrogen bonds, so that the stability is improved.

The present invention relates to a method for producing steviol glycosides of Stevia rebaudiana, which method comprises the following steps: preparing the Stevia rebaudiana nodal explants having two internodes; propagating the explants in temporary immersion systems (SETIS) with controlled immersion cycles; adding an elicitor after 21 days of culture; and transferring to a liquid culture medium enriched with growth regulators and nutrients that are essential to plant growth and development.

The invention provides an application of sclerotinia sclerotiorum small peptide Xyn25 in improvement of plant disease resistance, and belongs to the technical field of biopesticides. The invention provides a sclerotinia sclerotiorum small peptide Xyn25. The amino acid sequence of the sclerotinia sclerotiorum small peptide Xyn25 is shown as SEQ ID NO: 1. The sclerotinia sclerotiorum small peptide Xyn25 can efficiently activate plant immune response and improve the disease resistance of plants. The embodiment of the invention shows that the sclerotinia sclerotiorum small peptide Xyn25 can improve the resistance of wheat to gibberellic disease and rice to banded sclerotial blight. On the basis, the invention further provides an immune resistance inducer which comprises the sclerotinia sclerotiorum small peptide Xyn25 and auxiliary materials. The immune resistance inducer provided by the invention meets the development requirements of pesticide reduction, quality improvement and efficiency improvement in green agriculture, and has a good application prospect in the aspects of reducing the dependency degree of chemical pesticides and the like.

The invention discloses a plant immune induced resistance protein Ps131903 and application thereof. The amino acid sequence of the immune induced resistance protein Ps131903 is shown as SEQ ID NO.2. The protein Ps131903 can reduce the harm of phytophthora sojae and pythium ultimum to soybeans, reduce the harm of phytophthora capsici to chilies and tomatoes and reduce the harm of pyricularia grisea to rice. The compound has the advantages of low use concentration, quick effect and broad spectrum, and provides a new way for improving the plant resistance, thereby having wide application prospects in agricultural production.

The present application relates to the field of genetic engineering, in particular to an immune elicitor GH20 gene and the application of the encoded protein thereof in the prevention and treatment of corn large spot disease. The nucleotide sequence of the immune elicitor GH20 gene is shown as SEQ ID NO. 1, and the amino acid sequence of the encoded protein is shown as SEQ ID NO. 2. The present application first discloses the biological function of the immune elicitor GH20 gene and the encoded protein thereof, and further verifies that the immune elicitor GH20 gene and the encoded protein thereof have significant prevention and treatment effects on corn large spot disease through prokaryotic expression, and can be widely applied in the biological prevention and treatment of corn large spot disease. It can be seen that the present application provides a new biological preparation for the prevention and treatment of corn large spot disease, and lays a good foundation for the creation and development of new biological pesticides.

Described are plant defense elicitors or plant elicitor compositions and their use in, for example, agricultural applications, more particularly, to protect plants against pests or pathogens. This includes the corresponding methods of and uses in the protection of plants and crops by application of these plant defense elicitors or plant elicitor compositions. The active ingredient(s) of the plant defense elicitor or plant elicitor comprise lignin-derived phenolic oligomers with a degree of polymerization (DP) of 2 to 8 (preferably 2-4) (such as lignin-derived dimeric (diphenolic), lignin-derived trimeric compounds (triphenolic) and lignin-derived tetrameric compounds) and the engineered or synthesized structurally similar compounds. More particularly, described is a plant defense elicitor wherein an active ingredient of the plant defense elicitor comprises depolymerized lignin containing as active ingredient lignin-derived phenolic oligomers (such as lignin-derived dimeric compounds (diphenolic), lignin-derived trimeric compounds (triphenolic), and lignin-derived tetrameric compounds) and engineered or synthesized structurally similar compounds.

The present invention discloses a strain of Phylloporia lonecerae, which is classified and named as Phylloporia lonecerae, and has been deposited in the China General Microbiological Culture Collection Center on November 22, 2023, with a deposit number of CGMCC No. 40990. At the same time, the present application discloses a culture method for increasing the mycelium yield of Phylloporia lonecerae, adding an elicitor to the culture medium, providing excellent culture conditions for Phylloporia lonecerae, and providing an experimental basis and technical support for the industrial production of Phylloporia lonecerae and the development and utilization of strain resources.

This invention discloses a green control method and application for banana wilt disease based on banana extracellular DNA, belonging to the field of plant disease biological control technology. The method includes: extracting total DNA from healthy banana tissue, obtaining esDNA fragments of 100-500 bp size through physical shearing; preparing the esDNA into a working solution, and applying it to banana plants via root irrigation. This invention utilizes banana-derived esDNA as an immune elicitor, which not only directly inhibits the mycelial growth of *Fusarium oxysporum* var. *cube* (especially Foc TR4), but also systematically activates the immune response of banana plants, enhances the activity of their defensive enzymes, and reduces the accumulation of reactive oxygen species, thereby significantly reducing rhizosphere colonization of the pathogen, alleviating yellowing and wilting symptoms, and improving plant survival rate and biomass. This method is environmentally friendly, highly safe, and easy to operate, providing a new green solution for the sustainable management of banana wilt disease.

Physiological processes in plants are regulated and reinforced, and crop vitality, yield, quality and post-harvesting storage life are improved. A plant vitalizer comprising an exogenous elicitor and an endogenous elicitor is applied to plants.