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65 results about "Rhodosporidium toruloides" patented technology
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Rhodosporidium toruloides is an oleaginous yeast. It is a red basidiomycete isolated from wood pulp from conifers. Rhodosporidium toruloides has been linked to bovine mastitis, but its epidemiology has not yet been reported. The anamorph associated with the species is Mycotorula rubescens (Saito) Cif. & Redaelli.
Orange biological preserving agent based on yeast, sodium bicarbonate and elicitor activity
InactiveCN102224843AInhibitionInhibition of developmentFruit and vegetables preservationSodium bicarbonateGram
The invention discloses an orange biological preserving agent based on yeast, sodium bicarbonate and elicitor activity, composed of cryptococcus laurentii suspension, rhodotorula glutinis suspension, rhodosporidium toruloides suspension, sodium bicarbonate, auxin, salicylic acid and water. The preserving agent has 1*1010-1*1012 cryptococcus laurentii cells, 1*1010-1*1012 rhodotorula glutinis cells, 1*1010-1*1012 rhodosporidium toruloides cells, 5-50 grams of sodium bicarbonate, 10-400mg of auxin, 1-100mg of salicylic acid and the reminder consisting of water per liter of the preserving agent. The accession number of cryptococcus laurentii is CGMCC No.3590, the accession number of rhodosporidium toruloides is IMI 394084, and the accession number of rhodotorula glutinis is CGMCC No.3589. The orange biological preserving agent disclosed in the invention can control orange postharvest diseases without using chemical bactericides.
Owner:ZHEJIANG UNIV
Orange biological preservative based on antagonistic activities of three antagonistic yeasts
InactiveCN102224842AIntegration activityInhibitionFruit and vegetables preservationDiseasePreservative
The invention discloses an orange biological preservative based on antagonistic activities of three antagonistic yeasts, composed of cryptococcus laurentii suspension, rhodotorula glutinis suspension, rhodosporidium toruloides suspension and water. The preserving agent has 1*1010-1*1011 cryptococcus laurentii cells, 1*1010-1*1011 rhodotorula glutinis cells, 1*1010-1*1011 rhodosporidium toruloides cells1 and the reminder consisting of water per liter of the preserving agent. The accession number of cryptococcus laurentii is CGMCC No.3590, the accession number of rhodosporidium toruloides is IMI 394084, and the accession number of rhodotorula glutinis is CGMCC No.3589. The orange biological preserving agent disclosed in the invention can control orange postharvest diseases without using chemical bactericides.
Owner:ZHEJIANG UNIV
Orotate phosphoribosyltransferase promoter and its application and construct and vector
With the present invention, upstream sequence and downstream sequence of orotate phosphoribosyltransferase genomic DNA of rhodosporidium toruloides are amplified, biological information analysis and functional verification are performed for the amplified sequences to obtain the promoter and the terminator, wherein a target gene can be effectively expressed in the rhodosporidium toruloides throughthe promoter and terminator, such that the promoter and terminator can be applicable for the rhodosporidium toruloides genetic engineering operation and the strain improvement. The invention further relates to the DNA construct containing the components and the vector containing the components.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Rhodosporidium toruloides Y0 and application thereof
The invention discloses Rhodosporidium toruloides Y0 and application thereof. The Rhodosporidium toruloides Y0 is preserved in China Center for Type Culture Collection (CCTCC), Wuhan University, Wuhan City, China, on December 29, 2013, with an accession number of CCTCC No. M2013725. The Rhodosporidium toruloides Y0 has abundant nutrients, is applicable to production of functional bait additives for animals and can rapidly grow in mariculture waste water with mariculture waste water as a main nutriment source and 1 to 1.4% of cane sugar added according to a massic volume ratio as a supplementary carbon source; so the purposes of utilization of mariculture waste water, reduction of waste water discharge pollution and obtainment of high-value bait additives for aquatic livestock are achieved.
Owner:广州诺晶生物技术有限公司
Carbonyl reductase gene, codase, vector, strain and application of gene
The invention discloses a carbonyl reductase derived from Rhodosporidium toruloides ZJB14212, a gene, a vector, a strain and an application of the carbonyl reductase in the preparation of chiral drug intermediates. The above carbonyl reductase can biologically catalyze preparation of highly optically pure (S)-N,N-dimethyl-3-hydroxy-3-(2-thienyl)propionamide, (S)-N-methyl-3-hydroxy-3-(2-thienyl)propionamide, (R)-3-chloro-1-(2-thienyl)1-propanol, ethyl (S)-3-hydroxy-3-(2-thienyl)propanoate, (S)-3-hydroxy-3-(2-thienyl)propionitrile, tert-butyl 6-chloro-(3R,5S)-dihydroxyhexanoate, (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxyvaleryl]-4-phenyl-1,3-oxazolidine-2-one and methyl [S-(E)]-2-[3-[3-[2-(7-chloro-2-quinolyl)vinyl]phenyl]-3-hydroxypropyl]benzoate.
Owner:ZHEJIANG UNIV OF TECH
Fructose-1,6-bisphosphate aldolase promoter and its application and construct, vector
A promoter and a terminator which can be used for effectively expressing a target gene on rhodosporidium toruloides and can be therefore used for genetic engineering operation and strain improvement of the rhodosporidium toruloides are obtained by amplifying an upstream sequence and a downstream sequence of rhodosporidium toruloides fructose-1,6-diphosphate aldolase genome DNA (Deoxyribonucleic Acid) and performing biological information analysis and functional verification. The invention also relates to a DNA construct comprising the elements and a vector.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Method for treatment on 137Cs radioactive waste liquid by microbe adsorption
InactiveCN103755036ARadioactive decontaminationBiological water/sewage treatmentMicroorganismLiquid waste
The invention discloses a method for treatment on a 137Cs radioactive waste liquid by microbe adsorption. The method is characterized in that a pH value of the 137Cs radioactive waste liquid is adjusted to 2.5-9; rhodosporidium toruloides are added into the 137Cs radioactive waste liquid at a temperature of 5-40 DEG C; after 137Cs are fully adsorbed by rhodosporidium toruloides, the rhodosporidium toruloides are separated; the separated rhodosporidium toruloides with 137Cs are subjected to radiation-resistant storage; and the liquid satisfying radioactivity standards is directly discharged by a non-radioactive waste water discharge method. The method for treating the 137Cs radioactive waste liquid has a highest 137Cs adsorption rate of 95% and a wide application range. The method provides an effective means for 137Cs radioactive waste liquid treatment.
Owner:SICHUAN UNIV
Recombinant saccharomyces cerevisiae as well as construction method and application thereof
ActiveCN106929439AReduce pollutionOvercoming low utilization in difficult-to-access cellsFungiMicroorganism based processesBiotechnologyChemical synthesis
Owner:TIANJIN UNIV
Orotate phosphoribosyltransferase promoter, application, construct and vector
InactiveCN102268432BMicroorganism based processesDNA/RNA fragmentationInformation analysisDNA construct
With the present invention, upstream sequence and downstream sequence of orotate phosphoribosyltransferase genomic DNA of rhodosporidium toruloides are amplified, biological information analysis and functional verification are performed for the amplified sequences to obtain the promoter and the terminator, wherein a target gene can be effectively expressed in the rhodosporidium toruloides throughthe promoter and terminator, such that the promoter and terminator can be applicable for the rhodosporidium toruloides genetic engineering operation and the strain improvement. The invention further relates to the DNA construct containing the components and the vector containing the components.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Microbial preparation efficiently converting heavy metal cadmium in contaminated soil and preparation method thereof
The invention discloses a microbial preparation efficiently converting heavy metal cadmium in contaminated soil and a preparation method thereof. The microbial preparation is obtained by mixing and conducting enrichment cultivation on a flora 1 composed of candida, phialemonium, rhodosporidium toruloides and cryptococcus and a flora 2 composed of acidithiobacillu thiooxidans, acidithiobacillus caldus, leptospirum ferrooxidans, sulfobacillus thermosulfidooxidans, acidithiobacillus ferrooxidans and ferroplasma thermophilum. By using the microbial preparation for treating the cadmium contaminated soil, after the metal cadmium is converted to be a soluble state from an insoluble state, the removal rate is 70-82%, and the microbial preparation has the advantages of being high in conversion efficiency, short in cycle, low in cost, free of secondary pollution, easy to popularize and the like.
Owner:CENT SOUTH UNIV +1
Galactokinase promoter and terminator, and applications thereof
By amplifying the upstream and downstream sequences of genome DNA of galactokinase of Rhodosporidium toruloides, a promoter and a terminator are obtained. The promoter and terminator can be widely applied to gene expression, operation of genetic engineering, and strain improvement of Rhodosoporidium, Sporidiobolus, Sporobolomyces, and Rhodotorula. The nucleotide sequences of the promoter and the terminator are SEQ ID No.1 and SEQ ID No.2 respectively. The invention also relates to a DNA expression cassette or recombinant carrier comprising the elements, a method utilizing related elements to establish gene engineering strains of Rhodosoporidium, Sporidiobolus, and Rhodotorula, and corresponding strains.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Application of microbial preparation for efficiently converting heavy metal cadmium in polluted soil
The invention discloses an application of a microbial preparation for efficiently converting heavy metal cadmium in polluted soil. The microbial preparation is prepared by performing enriched cultivation on the following two mixed floras: (1) candida mycoderma bacteria, aspergillus monicus, rhodosporidium toruloides and cryptococcus which form a flora 1; and (2) acidithiobacillus thiooxidans, leptospirillum ferrooxidans, acidithiobacillus caldus, sulfobacillus thermosulfidooxidans, acidithiobacillus ferrooxidans and thermos ferric bacteria which form a flora 2. By using the microbial preparation to treat the cadmium polluted soil, the metal cadmium is converted from being indissolvable into being soluble and the removal rate is 70-82%. The microbial preparation disclosed by the invention has the advantages of being high in conversion efficiency, short in period, low in cost, free of secondary pollution, easy to popularize and the like.
Owner:HUNAN AGRI BIOTECH RES CENT +1
Strain for co-producing unsaturated fatty acid and carotenoid and application thereof
The invention relates to a strain for co-production of unsaturated fatty acid and carotenoid and an application of the strain. The strain is classified and named as Rhodosporidium toruloides Z11, and the preservation number of the strain is CCTCC NO: M 2021226. According to the invention, through ion beam mutagenesis, the obtained mutant strain takes glucose as a unique carbon source, and the grease content and carotenoid yield in shake flask fermentation respectively reach 66% and 13.5 mg / g, which are respectively 2.12 times and 12.85 times of those of an original strain cultured under the same condition; the strain is high in stability, high in product yield and good in repeatability, and is an excellent strain suitable for being used as animal feed. The strain is rich in a large amount of protein and a certain amount of vitamin carotenoid, as an animal additive, the preparation process is simple, the production raw material source is wide, large-scale production is facilitated, the problem that residual mushroom dregs after microbial oil extraction are not suitable for animals to digest and absorb is solved, meanwhile the production cost of the microbial oil is reduced, and comprehensive utilization of resources is promoted.
Owner:NANJING UNIV OF TECH
Production of microbial oils
InactiveUS20160145660A1Efficiently metabolizeFatty oils/acids recovery from wasteFungiCelluloseMicrobial oil
The present invention relates to the Rhodosporidium toruloides CECT 13085 strain, as well as to uses thereof for obtaining microbial biomass rich in triglycerides and for producing oils of a microbial origin in the presence of lignocellulosic biomass hydrolysates.
Owner:NEOL BIOSOLUTIONS
Orotidine-5'-phosphate decarboxylase gene, and protein and use thereof
ActiveCN101440375AIncrease production intensityImprove utilizationFermentationVector-based foreign material introductionBiotechnologyTotal rna
The invention relates to an orotidine-5'-phosphoric acid decarboxylase gene separated from rhodosporidum toruloides and coding albumen and a recombined vector thereof. The rhodosporidum toruloides AS2.0389 total RNA is taken as a template, the degeneracy PCR and RACE methods are used, Rt-ura3 and cDNA sequences which are as shown in SEQ ID No:1 are obtained by separation, a genome DNA sequence is as shown in SEQ ID No:3, and an amino acid sequence of the coding albumen is as shown in SEQ ID No:2. The obtained vector pYES2 / CT-Rt-ura3 can be used for compensating biosynthesis way of pyrimidine of auxotroph distillers yeast BY4741, and makes constructed engineering strain normally grow in a culture medium without containing uracil. When the Rt-ura3 gene is used as auxotroph marker gene or counter selection marker gene, a new yeast genetic operation system and a new recombined engineering strain can be constructed.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Method for enhancing production of alpha-linolenic acid from rhodosporidium toruloides
ActiveCN105368885AIncrease productionImprove use valueMicroorganism based processesFermentationSucroseBatch fermentation
The invention provides a method for enhancing production of alpha-linolenic acid from rhodosporidium toruloides. The method comprises the following steps: 1) culturing activated rhodosporidium toruloides in a YEPD culture medium; and 2) collecting a rhodosporidium toruloides strain cultured in the step 1), inoculating the strain to a fed-batch fermentation culture medium and conducting fed-batch fermentation, wherein the fed-batch fermentation culture medium consists of a carbon source, a nitrogen source and inorganic salt; preferably, the carbon source is a mixed carbon sucrose containing glucose and xylitol; and during fed-batch fermentation process, a total carbon-to-nitrogen ratio is constantly kept at 100 to (0.7-1.5). The method disclosed by the invention is simple to operate and easy to control; and the yield of alpha-linolenic acid can reach more than 5% of total lipid.
Owner:江苏东誉绿塑生物科技有限公司
Tea residue fermentation broth and preparation method and application thereof
The invention discloses a tea residue fermentation broth and preparation method and application thereof. The preparation method comprises the steps that (1) tea residue ishydrolyzedto obtain a tea residue hydrolysate; (2) rhodosporidium toruloides is activated in a YEPD medium to obtain the activated rhodosporidium toruloides; (3) the activated rhodosporidium toruloidesis inoculated and cultured in the tea residue hydrolysate, and bacterial cells are collected after separation; (4) the bacterial cells are inoculated into the tea residue hydrolysate culture medium for expandedfermental cultivation; and the tea residue hydrolysate medium contains the tea residue hydrolysate and inorganic salts. According to the tea residue fermentation broth and the preparation method and application thereof,the tea residue hydrolysateand therhodosporidium toruloideswhich are cultured by the tea residue hydrolysate and capable of being directly used as feed additives for laying henscan be obtained,.
Owner:FUJIAN NORMAL UNIV
Growth-promoting bacterial strain for promoting rooting of cutting tea seedlings and microbial seedling culturing substrate thereof
InactiveCN107099465APromote growthGood growth promoting effectBiocidePlant growth regulatorsMicroorganismRoot growth
The invention belongs to the technical field of tea cultivation and particularly relates to a growth-promoting bacterial strain for promoting the rooting of cutting tea seedlings and a microbial seedling culturing substrate thereof. The growth-promoting bacterial strain is bacterial strain WYS-2 belonging to Rhodosporidium toruloides and preserved in CGMCC (China General Microbiological Culture Collection Center) with the culture preservation number of CGMCC NO.13524 on January 3rd 2017. Above 1*108cfu / g of colony-forming unit of the bacterial strain WYS-2 is contained in the microbial seedling culturing substrate. Alumite and the tea root growth-promoting bacterial strain WYS-2 are firstly obtained and the microbial seedling culturing substrate is prepared later. By means of the microbial seedling culturing substrate, the rooting time of cutting tea seedlings is obviously shortened; the callus rate and the rooting percentage of the cutting tea seedlings are obviously increased; the tea seedling culturing period is shorter; and the seedling culturing efficiency is higher.
Owner:TEA RES INST ANHUI ACAD OF AGRI SCI
Glucose derepression induction promoter and terminator, and applications of promoter and terminator
A promoter and a terminator which can be widely applied to gene expression, genetic engineering operation and strain improvement of Rhodosoporidium, Sporidiobolus, Sporobolomyces and Rhodotorula in red yeast through amplifying the upstream and downstream sequences of Rhodosporidium toruloides glucose derepression induction alcohol dehydrogenase Adh2 genome DNA and carrying out biological information analysis and functional verification, the nucleotide sequence of the promoter is represented by SEQ ID NO:1, and the nucleotide sequence of the terminator is represented by SEQ ID NO:2. The invention also relates to a DNA expression box or a recombinant vector containing above elements, a method using the correlated elements to construct Rhodosoporidium, Sporidiobolus, Sporobolomyces and Rhodotorula gene engineering strains, and corresponding strains.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Rhodosporidium toruloides recombinant expression strain with high yield of ergothioneine and construction method and application of rhodosporidium toruloides recombinant expression strain
PendingCN114277042AShort fermentation cycleLow difficulty in transformationFungiMicroorganism based processesBiotechnologyMicrobiology
The invention discloses a rhodosporidium toruloides recombinant expression strain with high yield of ergothioneine as well as a construction method and application of the rhodosporidium toruloides recombinant expression strain. The method comprises the following steps: introducing and integrating ergothioneine synthesis genes egt1 and egt2 obtained by cloning from Neurospora crassa into a rhodosporidium toruloides genome, and screening to obtain a high-yield ergothioneine strain. Compared with an original strain, the yield of ergothioneine fermented by a 5L fermentation tank of the recombinant strain is increased by 270.2% compared with that of the original strain and reaches about 8.5 g / L, and a good foundation is laid for industrial production of ergothioneine by the Rhodosporidium torulosporidium strain. Meanwhile, the fermentation period is short, fermentation products are free of toxic and side effects, the metabolic pathway transformation difficulty of the rhodotorula glutinis is small, and operability is higher.
Owner:INNER MONGOLIA KINGDOMWAY PHARMA LTD +2
Galactokinase promoter and terminator, and application of promoter and terminator
A promoter and a terminator which can be widely applied to gene expression, genetic engineering operation and strain improvement of Rhodosoporidium, Sporidiobolus, Sporobolomyces and Rhodotorula in red yeast through amplifying the upstream and downstream sequences of Rhodosporidium toruloides galactokinase genome DNA and carrying out biological information analysis and functional verification, the nucleotide sequence of the promoter is represented by SEQ ID NO:1, and the nucleotide sequence of the terminator is represented by SEQ ID NO:2. The invention also relates to DNA expression box or a recombinant vector containing above elements, a method using the correlated elements to construct Rhodosoporidium, Sporidiobolus, Sporobolomyces and Rhodotorula gene engineering strains, and corresponding strains.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Rhodosporidium toruloides strain and application thereof to production of torularhodin
ActiveCN109401988ARaise the ratioIncrease productionFungiMicroorganism based processesRhodotorulaWild type
The invention discloses a rhodosporidium toruloides strain and application thereof to production of torularhodin. According to the rhodosporidium toruloides strain, an agrobacterium-mediated transformation method is applied to leading to mutation of wild rhodosporidium toruloides np11 to obtain the rhodosporidium toruloides strain A1-15-BRQ with a preservation number of CGMCC (China General Microbiological Culture Collection Center) NO. 16638. Application of the rhodosporidium toruloides strain A1-15-BRQ to production of torularhodin comprises S1, activating bacterial seeds to obtain a seed solution; S2, inoculating the seed solution into a liquid culture medium for cultivation at 28-30 DEG C and 180-200 rpm / min for 48-96 h to obtain a fermentation solution; during application, the fermentation solution can improve the production proportion and the yield of torularhodin by 1.5-2.5 times and 4.0-9.6 times respectively compared with the wild rhodosporidium toruloides np11, thereby beingapplicable to industrial production of torularhodin and having a good application prospect and high economic values.
Owner:DALIAN POLYTECHNIC UNIVERSITY
Novel application of delta12-fatty acid desaturase gene
InactiveCN104894146AImprove qualityLow costFermentationVector-based foreign material introductionAlpha-Linolenic acidGene
Owner:KUNMING UNIV OF SCI & TECH
Process for the production of (S)-5-chloro-2-isopropylpent-4-enoic acid esters
A process for preparing a compound of formula (1) in enantiomerically enriched form, which comprises selective hydrolysis of the corresponding racemic compound (2) catalysed by an enzyme derived from a non-mammalian source and having esterase or lipase activity, wherein R is methyl or C2-10 linear or branched alkyl. The non-mammalian source is a preferably yeast from the genus Rhodosporidium, preferably Rhodosporidium toruloides, in particular Rhodosporidium toruloides CMC 103105 or CMC 103107
Owner:TAYLOR IAN N
Rhodosporidium toruloides promoter and application thereof
ActiveCN114540356AAchieving targeted knockoutPeptidesVector-based foreign material introductionNucleotideNucleotide sequencing
The invention discloses a rhodosporidium promoter. The nucleotide sequences of the rhodosporidium promoter are shown as SEQ ID NO: 1 and SEQ ID NO: 2; the two U6 gene promoters obtained from the rhodosporidium are applied to a CRISPR / Cas9 gene editing system of the rhodosporidium, the expression of gRNA in the system is guided, efficient fixed-point gene knockout is achieved, a set of efficient genetic manipulation tool is provided for the rhodosporidium, and important research significance and application value are achieved.
Owner:KUNMING UNIV OF SCI & TECH
Na<+>/Pi cotransporter promoter and terminator as well as application thereof
The present invention amplifies Rhodosporidium toruloides Na + / Pi co-transporter genomic DNA upstream and downstream sequences, biological information analysis and functional verification, obtained can be widely used in Rhodosoporidium (Rhodosoporidium), lock throwing yeast (Sporidiobolus), throwing yeast ( Sporobolomyces) and Rhodotorula (Rhodotorula) gene expression, genetic engineering operations and strain improvement promoter and terminator, the nucleotide sequences of which are SEQ ID NO: 1 and SEQ ID NO: 2, respectively. The present invention also relates to a DNA expression cassette or a recombinant vector containing these elements, and a method for constructing Rhodosporidium, Saccharomyces and Rhodotorula genetically engineered strains using related elements and corresponding bacterial strains.
Owner:DALIAN INST OF CHEM PHYSICS CHINESE ACAD OF SCI
Rhodosporidium toruloides for producing linoleic acid and linolenic acid and preparing method thereof
ActiveCN105950494AIncrease productionImprove use valueFungiNucleic acid vectorAlpha-Linolenic acidHigh activity
The invention discloses rhodosporidium toruloides for producing linoleic acid and linolenic acid and a preparing method thereof. High-activity delta 12 desaturase gene obtained through intracellular cloning in Mortierella alpine is connected to an improved plant binary expression vector pCAMBIA1300 and transferred into Agrobacterium tumefaciens LBA4404 cells, the delta 12 desaturase gene is combined into a genome of rhodosporidium toruloides through co-culture to achieve overexpression of the Mortierella alpine delta 12 desaturase in rhodosporidium toruloides cells, and therefore the capacity for producing linoleic acid and alpha-linolenic acid through rhodosporidium toruloides can be enhanced.
Owner:FUJIAN MINDONG REJUVENATION PHARMA
Method for efficient conversion of soil weak-acid-bound cadmium by compoundbacterial agent
The invention relates to a method for efficient conversion of soil weak-acid-bound cadmium by compoundbacterial agent. The compound bacterial agent is obtained by mixing and enrichment culture of two floras as follows: (1) aflora I comprising candida, phialemonium,rhodosporidium toruloides and cryptococcus; (2) a flora II comprising acidithiobacillus thiooxidans, leptospirillum ferrooxidans, acidithiobacillus caldus,sulfobacillus thermosulfidooxidans, acidithiobacillus ferrooxidansand ferroplasma thermophilum. The obtained compoundbacterial agent is guided into cadmium polluted soil after culture, cadmium is fully reacted after stirring for a certain time, and cadmium can be efficiently converted into soil weak-acid-bound cadmium.
Owner:HUNAN AGRI BIOTECH RES CENT +1
Carbonyl reductase gene, encoding enzyme, vector, strain and application
The invention discloses a carbonyl reductase derived from Rhodosporidium toruloides ZJB14212, a gene, a vector, a strain and an application of the carbonyl reductase in the preparation of chiral drug intermediates. The above carbonyl reductase can biologically catalyze preparation of highly optically pure (S)-N,N-dimethyl-3-hydroxy-3-(2-thienyl)propionamide, (S)-N-methyl-3-hydroxy-3-(2-thienyl)propionamide, (R)-3-chloro-1-(2-thienyl)1-propanol, ethyl (S)-3-hydroxy-3-(2-thienyl)propanoate, (S)-3-hydroxy-3-(2-thienyl)propionitrile, tert-butyl 6-chloro-(3R,5S)-dihydroxyhexanoate, (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxyvaleryl]-4-phenyl-1,3-oxazolidine-2-one and methyl [S-(E)]-2-[3-[3-[2-(7-chloro-2-quinolyl)vinyl]phenyl]-3-hydroxypropyl]benzoate.
Owner:ZHEJIANG UNIV OF TECH
Diacylglycerol Acyltransferase (dga1) polynucleotides, and methods of increasing lipid production in yeast cells by overexpression of heterologous dga1
DGA1 catalyzes the final enzymatic step in the conversion of acyl-CoA and 1,2-diacylglycerol to triacylglycerol (TAG) and CoA in yeast. Methods of expressing a polynucleotide sequence encoding DGA1 from Rhodosporidium toruloides, Liposporidium stada, Schizochytrium, Aspergillus terreus, or Ergot in an oleaginous yeast host are disclosed. Also described herein are engineered recombinant host cells of Yarrowia lipolytica comprising a heterologous DGA1 polynucleotide encoding a DGA1 protein or a functionally active portion thereof, having the ability to produce increased lipid production and having increased glucose consumption efficiency feature.
Owner:GINKGO BIOWORKS INC
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