65 results about "Rhodosporidium toruloides" patented technology

The invention discloses an orange biological preserving agent based on yeast, sodium bicarbonate and elicitor activity, composed of cryptococcus laurentii suspension, rhodotorula glutinis suspension, rhodosporidium toruloides suspension, sodium bicarbonate, auxin, salicylic acid and water. The preserving agent has 1*1010-1*1012 cryptococcus laurentii cells, 1*1010-1*1012 rhodotorula glutinis cells, 1*1010-1*1012 rhodosporidium toruloides cells, 5-50 grams of sodium bicarbonate, 10-400mg of auxin, 1-100mg of salicylic acid and the reminder consisting of water per liter of the preserving agent. The accession number of cryptococcus laurentii is CGMCC No.3590, the accession number of rhodosporidium toruloides is IMI 394084, and the accession number of rhodotorula glutinis is CGMCC No.3589. The orange biological preserving agent disclosed in the invention can control orange postharvest diseases without using chemical bactericides.

The invention discloses an orange biological preservative based on antagonistic activities of three antagonistic yeasts, composed of cryptococcus laurentii suspension, rhodotorula glutinis suspension, rhodosporidium toruloides suspension and water. The preserving agent has 1*1010-1*1011 cryptococcus laurentii cells, 1*1010-1*1011 rhodotorula glutinis cells, 1*1010-1*1011 rhodosporidium toruloides cells1 and the reminder consisting of water per liter of the preserving agent. The accession number of cryptococcus laurentii is CGMCC No.3590, the accession number of rhodosporidium toruloides is IMI 394084, and the accession number of rhodotorula glutinis is CGMCC No.3589. The orange biological preserving agent disclosed in the invention can control orange postharvest diseases without using chemical bactericides.

With the present invention, upstream sequence and downstream sequence of orotate phosphoribosyltransferase genomic DNA of rhodosporidium toruloides are amplified, biological information analysis and functional verification are performed for the amplified sequences to obtain the promoter and the terminator, wherein a target gene can be effectively expressed in the rhodosporidium toruloides throughthe promoter and terminator, such that the promoter and terminator can be applicable for the rhodosporidium toruloides genetic engineering operation and the strain improvement. The invention further relates to the DNA construct containing the components and the vector containing the components.

The invention discloses Rhodosporidium toruloides Y0 and application thereof. The Rhodosporidium toruloides Y0 is preserved in China Center for Type Culture Collection (CCTCC), Wuhan University, Wuhan City, China, on December 29, 2013, with an accession number of CCTCC No. M2013725. The Rhodosporidium toruloides Y0 has abundant nutrients, is applicable to production of functional bait additives for animals and can rapidly grow in mariculture waste water with mariculture waste water as a main nutriment source and 1 to 1.4% of cane sugar added according to a massic volume ratio as a supplementary carbon source; so the purposes of utilization of mariculture waste water, reduction of waste water discharge pollution and obtainment of high-value bait additives for aquatic livestock are achieved.

A promoter and a terminator which can be used for effectively expressing a target gene on rhodosporidium toruloides and can be therefore used for genetic engineering operation and strain improvement of the rhodosporidium toruloides are obtained by amplifying an upstream sequence and a downstream sequence of rhodosporidium toruloides fructose-1,6-diphosphate aldolase genome DNA (Deoxyribonucleic Acid) and performing biological information analysis and functional verification. The invention also relates to a DNA construct comprising the elements and a vector.

The invention discloses a method for treatment on a 137Cs radioactive waste liquid by microbe adsorption. The method is characterized in that a pH value of the 137Cs radioactive waste liquid is adjusted to 2.5-9; rhodosporidium toruloides are added into the 137Cs radioactive waste liquid at a temperature of 5-40 DEG C; after 137Cs are fully adsorbed by rhodosporidium toruloides, the rhodosporidium toruloides are separated; the separated rhodosporidium toruloides with 137Cs are subjected to radiation-resistant storage; and the liquid satisfying radioactivity standards is directly discharged by a non-radioactive waste water discharge method. The method for treating the 137Cs radioactive waste liquid has a highest 137Cs adsorption rate of 95% and a wide application range. The method provides an effective means for 137Cs radioactive waste liquid treatment.

The invention relates to the technical field of genetic engineering and discloses a recombinant saccharomyces cerevisiae strain as well as a construction method and application thereof. The recombinant saccharomyces cerevisiae realizes production of 8-dimethyl isopentenyl naringenin with tyrosine as a substrate, in the recombinant saccharomyces cerevisiae strain, rhodosporidium toruloides is sourced from a TAL gene, parsley is sourced from the TAL gene, arabidopsis is sourced from a CHS gene, sweet mandarin is sourced from a CHI gene and sophora flavescens is sourced from an N8DT gene combination, thereby having the effect of producing the 8-dimethyl isopentenyl naringenin with high yield, and experiments show that the yield of the 8-dimethyl isopentenyl naringenin can reach 36.7mu g/L. The recombinant saccharomyces cerevisiae strain is utilized for producing the 8-dimethyl isopentenyl naringenin, cost is low, byproducts are fewer and pollution is low compared with plant extraction and chemical synthesis, a feasible method is provided for production of the 8-dimethyl isopentenyl naringenin and related products thereof, and a foundation is laid for biosynthesis of icaritin and icariin.

The invention discloses a microbial preparation efficiently converting heavy metal cadmium in contaminated soil and a preparation method thereof. The microbial preparation is obtained by mixing and conducting enrichment cultivation on a flora 1 composed of candida, phialemonium, rhodosporidium toruloides and cryptococcus and a flora 2 composed of acidithiobacillu thiooxidans, acidithiobacillus caldus, leptospirum ferrooxidans, sulfobacillus thermosulfidooxidans, acidithiobacillus ferrooxidans and ferroplasma thermophilum. By using the microbial preparation for treating the cadmium contaminated soil, after the metal cadmium is converted to be a soluble state from an insoluble state, the removal rate is 70-82%, and the microbial preparation has the advantages of being high in conversion efficiency, short in cycle, low in cost, free of secondary pollution, easy to popularize and the like.

By amplifying the upstream and downstream sequences of genome DNA of galactokinase of Rhodosporidium toruloides, a promoter and a terminator are obtained. The promoter and terminator can be widely applied to gene expression, operation of genetic engineering, and strain improvement of Rhodosoporidium, Sporidiobolus, Sporobolomyces, and Rhodotorula. The nucleotide sequences of the promoter and the terminator are SEQ ID No.1 and SEQ ID No.2 respectively. The invention also relates to a DNA expression cassette or recombinant carrier comprising the elements, a method utilizing related elements to establish gene engineering strains of Rhodosoporidium, Sporidiobolus, and Rhodotorula, and corresponding strains.

The invention discloses an application of a microbial preparation for efficiently converting heavy metal cadmium in polluted soil. The microbial preparation is prepared by performing enriched cultivation on the following two mixed floras: (1) candida mycoderma bacteria, aspergillus monicus, rhodosporidium toruloides and cryptococcus which form a flora 1; and (2) acidithiobacillus thiooxidans, leptospirillum ferrooxidans, acidithiobacillus caldus, sulfobacillus thermosulfidooxidans, acidithiobacillus ferrooxidans and thermos ferric bacteria which form a flora 2. By using the microbial preparation to treat the cadmium polluted soil, the metal cadmium is converted from being indissolvable into being soluble and the removal rate is 70-82%. The microbial preparation disclosed by the invention has the advantages of being high in conversion efficiency, short in period, low in cost, free of secondary pollution, easy to popularize and the like.

The invention relates to a strain for co-production of unsaturated fatty acid and carotenoid and an application of the strain. The strain is classified and named as Rhodosporidium toruloides Z11, and the preservation number of the strain is CCTCC NO: M 2021226. According to the invention, through ion beam mutagenesis, the obtained mutant strain takes glucose as a unique carbon source, and the grease content and carotenoid yield in shake flask fermentation respectively reach 66% and 13.5 mg/g, which are respectively 2.12 times and 12.85 times of those of an original strain cultured under the same condition; the strain is high in stability, high in product yield and good in repeatability, and is an excellent strain suitable for being used as animal feed. The strain is rich in a large amount of protein and a certain amount of vitamin carotenoid, as an animal additive, the preparation process is simple, the production raw material source is wide, large-scale production is facilitated, the problem that residual mushroom dregs after microbial oil extraction are not suitable for animals to digest and absorb is solved, meanwhile the production cost of the microbial oil is reduced, and comprehensive utilization of resources is promoted.

The present invention relates to the Rhodosporidium toruloides CECT 13085 strain, as well as to uses thereof for obtaining microbial biomass rich in triglycerides and for producing oils of a microbial origin in the presence of lignocellulosic biomass hydrolysates.

The invention relates to an orotidine-5'-phosphoric acid decarboxylase gene separated from rhodosporidum toruloides and coding albumen and a recombined vector thereof. The rhodosporidum toruloides AS2.0389 total RNA is taken as a template, the degeneracy PCR and RACE methods are used, Rt-ura3 and cDNA sequences which are as shown in SEQ ID No:1 are obtained by separation, a genome DNA sequence is as shown in SEQ ID No:3, and an amino acid sequence of the coding albumen is as shown in SEQ ID No:2. The obtained vector pYES2/CT-Rt-ura3 can be used for compensating biosynthesis way of pyrimidine of auxotroph distillers yeast BY4741, and makes constructed engineering strain normally grow in a culture medium without containing uracil. When the Rt-ura3 gene is used as auxotroph marker gene or counter selection marker gene, a new yeast genetic operation system and a new recombined engineering strain can be constructed.

The invention belongs to the technical field of tea cultivation and particularly relates to a growth-promoting bacterial strain for promoting the rooting of cutting tea seedlings and a microbial seedling culturing substrate thereof. The growth-promoting bacterial strain is bacterial strain WYS-2 belonging to Rhodosporidium toruloides and preserved in CGMCC (China General Microbiological Culture Collection Center) with the culture preservation number of CGMCC NO.13524 on January 3rd 2017. Above 1*108cfu/g of colony-forming unit of the bacterial strain WYS-2 is contained in the microbial seedling culturing substrate. Alumite and the tea root growth-promoting bacterial strain WYS-2 are firstly obtained and the microbial seedling culturing substrate is prepared later. By means of the microbial seedling culturing substrate, the rooting time of cutting tea seedlings is obviously shortened; the callus rate and the rooting percentage of the cutting tea seedlings are obviously increased; the tea seedling culturing period is shorter; and the seedling culturing efficiency is higher.

A promoter and a terminator which can be widely applied to gene expression, genetic engineering operation and strain improvement of Rhodosoporidium, Sporidiobolus, Sporobolomyces and Rhodotorula in red yeast through amplifying the upstream and downstream sequences of Rhodosporidium toruloides glucose derepression induction alcohol dehydrogenase Adh2 genome DNA and carrying out biological information analysis and functional verification, the nucleotide sequence of the promoter is represented by SEQ ID NO:1, and the nucleotide sequence of the terminator is represented by SEQ ID NO:2. The invention also relates to a DNA expression box or a recombinant vector containing above elements, a method using the correlated elements to construct Rhodosoporidium, Sporidiobolus, Sporobolomyces and Rhodotorula gene engineering strains, and corresponding strains.

The invention discloses a rhodosporidium toruloides recombinant expression strain with high yield of ergothioneine as well as a construction method and application of the rhodosporidium toruloides recombinant expression strain. The method comprises the following steps: introducing and integrating ergothioneine synthesis genes egt1 and egt2 obtained by cloning from Neurospora crassa into a rhodosporidium toruloides genome, and screening to obtain a high-yield ergothioneine strain. Compared with an original strain, the yield of ergothioneine fermented by a 5L fermentation tank of the recombinant strain is increased by 270.2% compared with that of the original strain and reaches about 8.5 g/L, and a good foundation is laid for industrial production of ergothioneine by the Rhodosporidium torulosporidium strain. Meanwhile, the fermentation period is short, fermentation products are free of toxic and side effects, the metabolic pathway transformation difficulty of the rhodotorula glutinis is small, and operability is higher.

A promoter and a terminator which can be widely applied to gene expression, genetic engineering operation and strain improvement of Rhodosoporidium, Sporidiobolus, Sporobolomyces and Rhodotorula in red yeast through amplifying the upstream and downstream sequences of Rhodosporidium toruloides galactokinase genome DNA and carrying out biological information analysis and functional verification, the nucleotide sequence of the promoter is represented by SEQ ID NO:1, and the nucleotide sequence of the terminator is represented by SEQ ID NO:2. The invention also relates to DNA expression box or a recombinant vector containing above elements, a method using the correlated elements to construct Rhodosoporidium, Sporidiobolus, Sporobolomyces and Rhodotorula gene engineering strains, and corresponding strains.

The invention discloses novel application of a delta12-fatty acid desaturase gene and discloses novel application to encoding of a sequence of a delta12-fatty acid desaturase gene separated from rhodosporidium toruloides YM25235, namely application to production of polyunsaturated fatty acids. A gene encoding product has the activity of delta15-fatty acid desaturase and can catalyze linoleic acid to be converted into alpha-linolenic acid.

The invention discloses a rhodosporidium promoter. The nucleotide sequences of the rhodosporidium promoter are shown as SEQ ID NO: 1 and SEQ ID NO: 2; the two U6 gene promoters obtained from the rhodosporidium are applied to a CRISPR/Cas9 gene editing system of the rhodosporidium, the expression of gRNA in the system is guided, efficient fixed-point gene knockout is achieved, a set of efficient genetic manipulation tool is provided for the rhodosporidium, and important research significance and application value are achieved.

The invention discloses rhodosporidium toruloides for producing linoleic acid and linolenic acid and a preparing method thereof. High-activity delta 12 desaturase gene obtained through intracellular cloning in Mortierella alpine is connected to an improved plant binary expression vector pCAMBIA1300 and transferred into Agrobacterium tumefaciens LBA4404 cells, the delta 12 desaturase gene is combined into a genome of rhodosporidium toruloides through co-culture to achieve overexpression of the Mortierella alpine delta 12 desaturase in rhodosporidium toruloides cells, and therefore the capacity for producing linoleic acid and alpha-linolenic acid through rhodosporidium toruloides can be enhanced.