Orange biological preserving agent based on yeast, sodium bicarbonate and elicitor activity

Abstract

The invention discloses an orange biological preserving agent based on yeast, sodium bicarbonate and elicitor activity, composed of cryptococcus laurentii suspension, rhodotorula glutinis suspension, rhodosporidium toruloides suspension, sodium bicarbonate, auxin, salicylic acid and water. The preserving agent has 1*1010-1*1012 cryptococcus laurentii cells, 1*1010-1*1012 rhodotorula glutinis cells, 1*1010-1*1012 rhodosporidium toruloides cells, 5-50 grams of sodium bicarbonate, 10-400mg of auxin, 1-100mg of salicylic acid and the reminder consisting of water per liter of the preserving agent. The accession number of cryptococcus laurentii is CGMCC No.3590, the accession number of rhodosporidium toruloides is IMI 394084, and the accession number of rhodotorula glutinis is CGMCC No.3589. The orange biological preserving agent disclosed in the invention can control orange postharvest diseases without using chemical bactericides.

Description

technical field

[0001] The invention relates to the technical field of post-harvest disease prevention and control of fruits, in particular to a biological antiseptic and fresh-keeping technology for preventing and curing citrus fruit diseases. Background technique

[0002] Chemical fungicides are currently the main and most effective method to control postharvest rot in citrus fruits. However, in view of the adverse effects of chemical fungicides on food safety and environmental pollution, the development of fresh-keeping technologies without fungicides is very important both at home and abroad. In particular, the technology of using safe and pathogenic yeasts to control fruit diseases is considered to be one of the most promising alternatives to chemical fungicides. However, compared with chemical fungicides, there is still a large gap in the control efficacy of existing biocontrol yeasts to diseases.

[0003] Cryptococcus laurentii is a postharvest biocontrol yeast stra...

Examples

preparation example Construction

[0026] The preparation methods of 3 kinds of yeast suspensions are as follows respectively:

[0027] The NYDA culture medium used below is: beef extract 8g, yeast powder 5g, glucose 10g, agar 20 grams, water is settled to 1000ml, high pressure (1kg / cm ) 121 ℃ of sterilization 30 minutes.

[0028] The NYDB seed culture medium used is: 8 g of beef extract, 5 g of yeast powder, 10 g of glucose, the volume of water is adjusted to 1000 ml, and high pressure (1 kg / cm 2 ) is sterilized at 121° C. for 30 minutes.

[0029] Cryptococcus laurentis, Rhodotorula viscosus, and Rhodosporidium were respectively stored in NYDA medium at low temperature (4°C). The training program is as follows:

[0030] ①. Activation: First culture in NYDA medium at 28°C for 48 hours, then repeat subculture twice (each time in NYDA medium at 28°C for 48 hours); activate the above-mentioned repeated subculture twice. The yeasts obtained from the NYDB seed medium were connected to the NYDB seed medium with an ...

Embodiment 1

[0034] Embodiment 1, the citrus biological preservative based on yeast, sodium bicarbonate and elicitor activity:

[0035] First, separately activate and cultivate Cryptococcus laurentis, Rhodotorula viscosus and Rhodosporidium; adjust the concentrations of the three yeasts to 2×10 9 cells / ml, take 0.5 liter volume of Cryptococcus lorensis suspension, add 0.5 liter volume of Rhodotorula viscosus suspension, stir and mix for 3 minutes; then add 0.5 liter volume of Rhodosporidium suspension, after the completion Continue to stir and mix for 3 minutes, slowly add 1000 ml of SA solution with a concentration of 1000 mg / L, stir and mix for 5 minutes after the SA solution is added; then slowly add 100 ml of IBA solution with a concentration of 1000 mg / L, and stir for 5 minutes Slowly add 2500 milliliters of sodium bicarbonate solution with a concentration of 80 g / L, stir and mix for 5 minutes and add water to dilute to 10 liters for subsequent use after the solution has been added. ...

Embodiment 2

[0036] Embodiment 2, adjust the consumption of SA solution, sodium bicarbonate solution and IBA solution among the embodiment 1; Thereby make the every L citrus biological preservative of final gained contain respectively 1 * 10 11 Cryptococcus laurentis, 1×10 11 sticky red yeast, 1 x 10 11 Rhodosporidium yeast cells, 50mg / L SA, 200mg / L IBA, 25g / L sodium bicarbonate, and the rest are water.

technical field

[0001] The invention relates to the technical field of post-harvest disease prevention and control of fruits, in particular to a biological antiseptic and fresh-keeping technology for preventing and curing citrus fruit diseases. Background technique

[0002] Chemical fungicides are currently the main and most effective method to control postharvest rot in citrus fruits. However, in view of the adverse effects of chemical fungicides on food safety and environmental pollution, the development of fresh-keeping technologies without fungicides is very important both at home and abroad. In particular, the technology of using safe and pathogenic yeasts to control fruit diseases is considered to be one of the most promising alternatives to chemical fungicides. However, compared with chemical fungicides, there is still a large gap in the control efficacy of existing biocontrol yeasts to diseases.

[0003] Cryptococcus laurentii is a postharvest biocontrol yeast stra...

Examples

preparation example Construction

[0026] The preparation methods of 3 kinds of yeast suspensions are as follows respectively:

[0027] The NYDA culture medium used below is: beef extract 8g, yeast powder 5g, glucose 10g, agar 20 grams, water is settled to 1000ml, high pressure (1kg / cm ) 121 ℃ of sterilization 30 minutes.

[0028] The NYDB seed culture medium used is: 8 g of beef extract, 5 g of yeast powder, 10 g of glucose, the volume of water is adjusted to 1000 ml, and high pressure (1 kg / cm 2 ) is sterilized at 121° C. for 30 minutes.

[0029] Cryptococcus laurentis, Rhodotorula viscosus, and Rhodosporidium were respectively stored in NYDA medium at low temperature (4°C). The training program is as follows:

[0030] ①. Activation: First culture in NYDA medium at 28°C for 48 hours, then repeat subculture twice (each time in NYDA medium at 28°C for 48 hours); activate the above-mentioned repeated subculture twice. The yeasts obtained from the NYDB seed medium were connected to the NYDB seed medium with an ...

Embodiment 1

[0034] Embodiment 1, the citrus biological preservative based on yeast, sodium bicarbonate and elicitor activity:

[0035] First, separately activate and cultivate Cryptococcus laurentis, Rhodotorula viscosus and Rhodosporidium; adjust the concentrations of the three yeasts to 2×10 9 cells / ml, take 0.5 liter volume of Cryptococcus lorensis suspension, add 0.5 liter volume of Rhodotorula viscosus suspension, stir and mix for 3 minutes; then add 0.5 liter volume of Rhodosporidium suspension, after the completion Continue to stir and mix for 3 minutes, slowly add 1000 ml of SA solution with a concentration of 1000 mg / L, stir and mix for 5 minutes after the SA solution is added; then slowly add 100 ml of IBA solution with a concentration of 1000 mg / L, and stir for 5 minutes Slowly add 2500 milliliters of sodium bicarbonate solution with a concentration of 80 g / L, stir and mix for 5 minutes and add water to dilute to 10 liters for subsequent use after the solution has been added. ...

Embodiment 2

[0036] Embodiment 2, adjust the consumption of SA solution, sodium bicarbonate solution and IBA solution among the embodiment 1; Thereby make the every L citrus biological preservative of final gained contain respectively 1 * 10 11 Cryptococcus laurentis, 1×10 11 sticky red yeast, 1 x 10 11 Rhodosporidium yeast cells, 50mg / L SA, 200mg / L IBA, 25g / L sodium bicarbonate, and the rest are water.

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